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Supplementary Data (S 1.). Small intestine epithelial cells. Lung cancer cells. Breast cancer cells. Colon cancer cells. Primary cells. COLO205. H157. MCF7. HUVEC. KM1214. SW403. CaCO2. RIE-1. H358. SMC. anti-UCH L1. anti-α- tubulin. Supplementary Data (S 2.). a. immediately. - PowerPoint PPT Presentation
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anti-UCH L1
anti-α-tubulin
H157
H358
SW40
3
COLO205
KM12
14
CaCO2
RIE-1
SMC
HUVEC
MCF7
Primary cells
Lung cancer cells
Small intestine epithelial cells
Colon cancer cells
Breast cancer cells
Supplementary Data (S 1.)
5h
71h
immediately
7h
b
a
control UCH-L1 RNAiuninfected
control UCH-L1 RNAiuninfected
Supplementary Data (S 2.)
anti UCH-L1
anti -tubulin
control UCH-L1 RNAi
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
control UCH-L1-myc
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
anti UCH-L1
anti -tubulin
UCH-L1-mycEndo UCH-L1
3 g 6 g
wc sol. insol. wc sol. insol. wc sol. insol.
Empty vector
anti UCH-L1
anti -tubulin
Flag-UCH-L1
Endo UCH-L1
Flag-UCH-L1
Supplementary Data (S 3.)
b
a
c
Supplementary Data (S 4.)
M1M2
M3
M1M2
M3
M1M2
M3
M1M2
M3
control UCH-L1 RNAicontrol UCH-L1 RNAi
control UCH-L1 RNAi
phase control UCH-L1 RNAi
G1 (M1) 46.59 47.69
S (M2) 22.18 20.60
G2/M (M3) 30.58 31.48
phase control UCH-L1 RNAi
G1 (M1) 54.73 56.30
S (M2) 17.04 16.15
G2/M (M3) 27.41 26.89
b
a