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Supplementary figure 1. A. B. Primary hippocampal neurons. Primary hippocampal neurons. D. E. Primary hippocampal neurons. Primary hippocampal neurons. Mouse ES cells. Mouse ES cells. NIH3T3 cells. NIH3T3 cells. Mouse ES cells. Mouse ES cells. NIH3T3 cells. NIH3T3 cells. C. - PowerPoint PPT Presentation
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Anti-Importin 3
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
Anti-Importin 1 Anti-Importin 1
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
Anti-E47
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
A B
C
D
Supplementary figure 1
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
E
Anti-NeuroD1
* *
*
**
Anti-GAPDH
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
F
Flag-NeuroD1
Flag-134-161
Anti-flag DAPI
Supplementary figure 2
GFP Injection Marker
NeuroD1-GFP
NeuroD1-GFP + RanQ69L-GTP
HeLa
A B
C D
Supplementary figure 3
NIH3T3
E F
HG
GFP Injection Marker
Supplementary figure 4
Anti-RanBP5 Anti-RanBP7
Neu
roD
1-G
ST
+cyt
osol
Neu
roD
1-G
ST
+cyt
osol
+R
anQ
69L
-
GTPHel
a ce
ll ly
sate
GS
T be
ads+
Cyt
osol
Neu
roD
1-G
ST
+cyt
osol
Neu
roD
1-G
ST
+cyt
osol
+R
anQ
69L
-
GTPHel
a ce
ll ly
sate
GS
T be
ads+
Cyt
osol
75100
150
A B
Supplementary figure 5
Importin 1 Importin + 1
(NeuroD1)
(A)
Database : NCBInr 20100904 (11744690 sequences; 4010973687 residues)Taxonomy : Homo sapiens (human) (232987 sequences)Timestamp : 8 Sep 2010 at 09:45:05 GMTTop Score : 182 for gi|19923142, importin subunit beta-1 [Homo sapiens]
Mascot Score Histogram
Protein score is -10*Log(P), where P is the probability that the observed match is a random event.Protein scores greater than 66 are significant (p<0.05)
Search Parameters
Enzyme : TrypsinFixed modifications : Carbamidomethyl (C)Variable modifications : Oxidation (M)Mass values : MonoisotopicProtein Mass : 95 kDaPeptide Mass Tolerance : ± 0.2 DaPeptide Charge State : 1+Max Missed Cleavages : 1Number of queries : 36
(B)
Supplementary figure 6