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Page 1 of 2 Application Key: WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation Species Reactivity Key: All-All Species A-Avian Amp-Amphibian Ar-Arabidopsis B-Bovine C-Canine Ch-Chicken D-Drosophila GP-Guinea Pig H-Human Ha-Hamster M-Mouse NHP- Non-human primate P-Pig R-Rat S-Sheep X-Xenopus Z-Zebrafish *Species assumed based on 100% homology with sequence used as antigen For Research Use Only
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Cite this Antibody: PhosphoSolutions Cat# 2020-TUB, RRID:AB_2492275 Host Applications Species Tested Species Assumed* Molecular Reference Mouse WB 1:10,000 M, R ~55 kDa IHC 1:1000 Product Description: Protein G purified mouse monoclonal antibody. Biological Significance: Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003). Antigen: Synthetic peptide corresponding to rat beta III tubulin. Antibody Specificity: Specific for endogenous levels of the ~55 kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1. Purification Method: Protein G purified culture supernatant. Quality Control Tests: Western blots performed on each lot. Packaging: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Storage and Stability: Shipped on blue ice. Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Anti-Beta III Tubulin Antibody Catalog #: 2020-TUB Size: 100 µl Isotype: IgG Clone: AA10
Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
Immunostaining of mouse dentate gyrus showing beta III tubulin in red and nuclei in blue. Photo courtesy of Robert Wine.
Page 2 of 2 Application Key: WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation Species Reactivity Key: All-All Species A-Avian Amp-Amphibian Ar-Arabidopsis B-Bovine C-Canine Ch-Chicken D-Drosophila GP-Guinea Pig H-Human Ha-Hamster M-Mouse NHP- Non-human primate P-Pig R-Rat S-Sheep X-Xenopus Z-Zebrafish *Species assumed based on 100% homology with sequence used as antigen For Research Use Only
Product Specific References: Cooper, R. J., & Spitzer, N. (2015). Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238. Ka, M., & Kim, W. Y. (2015). Microtubule-Actin Crosslinking Factor 1 Is Required for Dendritic Arborization and Axon Outgrowth in the Developing Brain. Molecular neurobiology, November, 1-15. General References: Lee MK, Rebhun LI, Frankfurter A (1990) Posttranslational modification of class III beta tubulin. Proc Natl Acad Sci USA 87(18): 7195-7199. Katsetos CD, Herman MM, Mork SJ (2003) Class III beta tubulin in human development and cancer. Cell Motil Cytoskeleton 55(2):77-96.
