Annual Report 2001 University of Pretoria · 2014-09-15 · 1 Jaarverslag / Annual Report 2001 University of Pretoria CONTENTS Message from the head of Department 2 Personnel 3 Extraordinary

1 Jaarverslag / Annual Report 2001

University of Pretoria CONTENTS Message from the head of Department 2

Personnel 3

Extraordinary Professors 4

Exceptional achiever 5

Promotions 6

New Appointments 8

Guest researchers and associates 8

Research awards and achievements 9

Overseas visits by staff & students 9

Plant Pathology Receives ISO 17025 10

Research Projects 11

Publications in science citation index journals 15

Contributions in published refereed conference proceedings 17

Chapters in refereed specialist books 17

National conference contributions 18

International conference contributions 22

MSc and PhD Degrees awarded 24

Post Graduate Students 25

Annual Report 2001


The department of Microbiology and Plant Pathology strives to be a dynamic multicultural institution which provides the highest standard, most relevant research and training in microbiology and plant pathology to satisfy education priorities in these fields, with excellence. These aspirations are reflected in the achievements of our staff and students. Dr Theron received an award from the University as an exceptional young scientist and Prof Korsten was promoted to full professor. Two post-graduate students received the Prestigious NRF

Scholarship and two received the Mellon foundation Award. Two new post-doc fellowship were also awarded. Over the past 5 years our student profile has been changing constantly at both the undergraduate and post graduate level and has become more representative of the South African demographic situation, with more than 30 % of our students hailing from previously disadvantaged communities. Research in the department is focused in the areas of water and environmental microbiology, plant biotechnology and molecular virology. The department has between 70 and 80 postgraduate students at any one time, with 48 % of the students in the department are post-graduate students. During the past 5 years 67 honours degrees were awarded and 44 students completed their MSc and PhD degrees, making the department a leader in the field of education and research in Microbiology and Plant Pathology in South Africa. More than 135 publications were published in peer reviewed scientific journals during the past 5 years and 6 patents registered. In the year 2001, 35 peer reviewed articles were published and the staff continued to make a significant contribution at both national and international scientific meetings, with a number of keynote presentations at international conferences. The strategic alliance between the CSIR and University of Pretoria, continues to impact positively on our department. Two PhD degrees and 20 MSc degrees were awarded in the joint (UP/CSIR) MSc Water Resource Management and PhD Water Resource Management program, since its inception two years ago and currently we have 10 MSc students and 2 PhD students enrolled in this program. Employment equity has been and still is high on the agenda in the department and much success has been achieved in addressing gender and race issues. Of the academic staff, 42 % currently represent designated groups. We are very fortunate to be associated with our extraordinary professors. Both Prof Steyn and Strydom play an important role in the Biological Nitrogen Fixation Center. Prof Jooste has played an important role in fostering the relationship between ourselves and the ARC and is currently involved in a joint NRF project. Prof Ashton is invaluable to the Center for Water Biotechnology as advisor, mentor and lecturer. Prof Atlas was elected as the President of the American Society for Microbiology during 2001. He furthermore co-authored a chapter in an Encyclopedia with Prof L H Nel. Prof Viljoen played an essential role in giving guidance to our students working at Onderstepoort Veterinary Research Institute. I would like to thank the staff and the students for making this an excellent department to lead.

Message from the Head of the Department – Prof T E Cloete


Doserende personeel / Lecturing staff Prof Cloete TE: MSc(UOVS) DSc(Pret) Pri Sci Nat – Professor and Head Prof Brözel VS: BSc (Food Science) (Stell) BSc (Hons)(Micro) MSc PhD(Pret) - Associate Professor Prof Coutinho TA: BSc, BSc Hons, MSc, PhD (Natal) – Associate Professor Prof Korsten L: BSc(Hons)(Stell), MSc PhD(Pret) - Professor Prof Nel LH: MSc(UOVS), PhD(Pret) - Professor Prof Steyn PL: MSc(Agric)(Pret) PhD(Calif) Pri Sci Nat - Adjunct Professor Prof Aveling TAS: MSc PhD(Natal) - Associate Professor Prof Wehner FC: MSc(Agric) DSc(Agric)(Pret) Pri Sci Nat – Professor Prof Wingfield MJ: BSc(Hons)(Natal) MSc(Stell) PhD(Minnesota) – Professor and Director: FABI Dr Labuschagne N: MSc(Agric) DSc(Agric)(Pret) Pri Sci Nat - Sen Lecturer Dr Theron J: BSc BSc(Hons) MSc PhD(Pret) – Sen Lecturer Dr Venter SN: MSc(Pret) – PhD Lecturer Dr Viljoen A: BSc, BSc (Hons) PhD (UOVS) – Sen Lecturer Mr Jaftha JB : MSc(Pret) - Jun Lecturer

Front: Mrs A Lombard, Ms M Engelbrecht, Mrs D Muller, Prof L Korsten, Ms K Dyk and Ms W Markotter. Middle row: Mrs A Bosch, Mrs B van Rooyen and Prof T E Cloete (Head of Department). Back row from left: Dr J Theron, Dr G Swart, Dr N Labuschagne, Dr F Venter, Prof T Aveling, Prof L H Nel, Ms M du Preez and Mr J Jaftha

PERSONNEL


Technical assistants Ms Kerien van Dyk: BSc (Agric Hons) Mrs Amelita Lombard: BSc (Agric Hons) Ms Wanda Markotter: BSc (Hons) UP Mrs Mariette Truter: BSc (Hons) UP Mrs Aida Bosch: Diploma in Mediese Tegnologie

Support Staff Mrs Madelein du Preez Mrs Lilian Motale Mrs Bettie van Rooyen Mr Albert Molefe Industry Funded Staff (Citrus and Subtropical) Dr G Swart: Research officer Ms A Redmond: Research Assistant Ms T Beart: Technical Assistant Ms D Muller: Administrative assistant

Prof P Ashton (CSIR); Prof R M Atlas (USA); Prof Hans -Curt Flemming (Germany); Prof P Jooste (ARC); Prof P L Steyn; Prof B Strydom; Prof G J Viljoen (Onderstepoort)

EXTRAORDINARY PROFESSORS


Doctor J Theron

Dr Theron recently received an award from the UP for exceptional achievement as a young scientist. Jacques Theron was born on 10 July 1968 in Pretoria and attended primary school in Paarl, near Cape Town and secondary school in Pretoria. He then enrolled at the University of Pretoria to study Microbiology. Having a healthy interest in sport, he represented the Northern Transvaal XV rugby team, but his interest in microbiology led to him enrolling in postgraduate studies at the University of Pretoria. He was awarded the M.Sc degree (cum laude) in 1994 and this was followed by a Ph.D in Microbiology in 1996.

During this time, his research focussed on the molecular biology of orbiviruses. After completion of his PhD, he was appointed as Lecturer in the Department of Microbiology and Plant Pathology at the University of Pretoria. At the beginning of 2000 he was promoted to the position of Senior Lecturer in this Department. His research interests straddle the fields of molecular bacteriology, virology and diagnostics. During 2000, he was the recipient of the Water Institute of southern Africa (WISA) biannual award for most promising young researcher and has recently received an award as an exceptional young researcher from the University of Pretoria (2002). He currently holds a Y1 peer rating which he received from the NRF in 2001. His current research interests include investigations regarding the molecular mechanisms underlying biofilm fomation by Gram positive and Gram negative bacteria (in collaboration with Prof V.S. Brözel), and the role of specific foot-and-mouth disease virus and orbivirus proteins in virus replication (in collaboration with Prof H. Huismans and Dr H.G. van Rensburg).

EXCEPTIONAL ACHIEVER


Professor Lise Korsten

Prof Korsten has recently been promoted to full professor in the department of Microbiology and Plant Pathology. Lise Korsten neé Myburgh was born in Stellenbosch, 10 February 1959. Her family later moved to Johannesburg where she attended Florida Primary and Florida High School on the Witwatersrand. She returned to Stellenbosch to study a BSc and BSc Hons degree which she completed in 1979/1980 majoring in Plant Pathology. Her primary focus was in the field of phytobacteriology studying under Prof Martin Hattingh. She left the University of Stellenbosch with a keen interest in mountaineering and wine appreciation and continued her studies at University of Pretoria. In 1984, she completed her Masters degree Cum Laude under the

guidance of Prof J M Kotzé (previous head of the department), Prof F C Wehner and Prof P L Steyn. During the same year she was appointed Research Officer in the department. Her studies focused on a new bacterial disease of avocado. She continued working on avocados but shifted focus to avocado sunblotch disease where she developed the first sunblotch viroid diagnostic center in South Africa in 1985. Thousands of nursery mother trees were subsequently tested for the South African Avocado industry laying a sound foundation for viroid-free propagative material. The dot blot hybridizations technique using radio-actively labeled RNA probes was optimised and commercially used in the newly fitted laboratory for radio active work which was equipped and funded by the SA avocado industry. In 1988, she was appointed Senior Lecturer and taught Microbial Ecology, Phytobacteriology and subsequently Introductory Plant Pathology. From there her interest in microbial ecosystems on plant surfaces started which laid the foundation for her PhD studies on biological control of avocado fruit diseases. In 1989 her firstborn son, Laurie arrived, putting an abrupt end to wild water kayaking and long distance running. In 1993 she obtained her PhD under Proff J M Kotzé and F C Wehner. She subsequently established a monoclonal unit on the ground floor which was later converted to a PCR laboratory. In 1997 she was promoted to Associate Professor and registered South Africa’s first biocontrol agent for preharvest applications to control fruit diseases. The product Avogreen, was subsequently commercialised by Stimuplant. In the same year her second son Louis-Neil, was born. Her research focus now included mango and citrus fruit diseases and an extended group of postgraduate students, researchers and assistants. Citrus greening, one of South Africa’s most devastating diseases, have been studied by her group since 1986 and resulted in the development of monoclonal antibodies and subsequently a PCR protocol for rapid field diagnosis of the pathogen Liberibacter africanis. A national field survey in 1994 revealed the presence of citrus greening (huanglongbing

PROMOTIONS


disease) in the Cape, a region previously regarded as greening free. In 1997, citrus black spot was included in the group’s research portfolio and soon a diagnostic PCR protocol was developed to distinguish between the pathogenic and non-pathogenic forms of Guignardia. Currently, South African citrus exporters face the possibility of losing an important export market in Europe due to the presence of this pathogen. More effective, accurate and rapid diagnosis of the pathogen can prevent major rejections of export consignments. Due to the recent global awareness of food safety in primary agriculture, detection and control of foodborne pathogens on farms and in packhouses was included in the postharvest research portfolio. Pos tgraduate training in Postharvest Technology was introduced by Prof Korsten and now includes Risk Assessment, Eurepgap, HACCP, and other pre -requisite programs for food safety. In addition, all six ground floor fruit laboratories equipped by the citrus and subtropical industries have been ISO 17025 accredited by SANAS. Prof Korsten’s team spent 18 months to develop and implement this system. All new students are currently trained in ISO and food safety systems empowering them to pursue a variety of career opportunities. Several techniques have been accredited which include bacterial identification, screening biocontrol agents, detection of foodborne pathogens in packhouse water, on fruit and in packhouses and PCR diagnostics. Prof Korsten entered the new millennium with her first daughter Carli and now fully understands the difficulty of balancing motherhood with an academic career. Prof Korsten is currently rated a B3 by the NRF and has trained one PhD and 17 MSc students. Currently she acts as promoter for 7 PhD and 11 MSc students.


Mariette Truter

Mariette matriculated at Oos-Moot high school and completed her BSc and BSc(Hons) degrees at the University of Pretoria. She is currently registered for a MSc degree in the Department of Microbiology and Plant Pathology. Her research mainly focuses on soilborne plant pathogens and has a keen interest in fungal taxonomy. She is responsible for presenting the practicals in the courses Fungal Taxonomy, Plant Disease Control, Introductory Crop Protection and Growth, Activity and Control of Fungi.

?? Dr P Ashton, Postgraduate Supervisor, CSIR, Environmentek, South Africa ?? Dr B Dungu-Kimbenga, Exotic Disease Division, Onderstepoort Vet Inst, Onderstepoort,

South Africa ?? Dr W Hammond, Biological Control Center for Africa, International Institute of Tropical

Agriculture, Benin ?? Dr R M Injairu, National Veterinary Research Centre, Kenya ?? Prof P Jooste, Postgraduate Supervisor, ARC, Meat Science, South Africa ?? Prof J van Buuren, Wageningen Agricultural University, The Netherlands ?? Prof G Viljoen, Onderstepoort Institute for Exotic Diseases, South Africa

GUEST RESEARCHERS AND ASSOCIATES

NEW APPOINTMENTS


Prof T A S Aveling was elected as Vice-chairperson: Seed Storage. Committee of the International Seed Testing Association.

Prof T E Cloete was elected to the International Water Association Strategic Council.

?? Prof V S Brözel visited the Group of Dr Thierry Jouenne, University of Rouen, France,

September 2001. ?? Prof T E Cloete attended the IWA Management and Policy Council Meeting, Windsor,

UK, May 2001. Prof Cloete was also a keynote speaker at the International Conference on Biofouling and Materials, Frankfurt am Main/DECHEMA House, June 2001. At the IWA Conference on Activated Sludge Microbial Population Dynamics he was an invited lecturer that was held in Rome, June 2001. He was also an Invited Speaker at the IWA World Congress, Berlin, October 2001 and Second International Symposium on Disinfection and Hygiene, Wageningen, October 2001.

?? Me Marleen Kock visited Dr Noëlle Amarger at the Laboratory de Microbiology des Sols, Institute National de la Recherche Agronomique in Dijon (France) as part of a research collaboration agreement.

?? Prof L Korsten visited Noordwijk, Netherland for the Noordwijk Food Safety and HACCP Forum from 15-17 March. Prof Korsten also visited Aachen, Germany in October 2001 for the EurepGAP training meeting and Bologne, Italy for the EurepGAP Congress also in October 2001.

?? Prof L H Nel visited the Center for disease control in Atlanta, April 2001. ?? Me Noelani van den Berg visited Scotland where she learned techniques on resistance gene

isolation and identification. ?? Dr A Viljoen and Edzard Grimbeek visited the National Agricultural Research

Organisation in Uganda for research related purposes, and Dr A Viljoen visited several laboratories and research institutes in Europe (Belgium, Netherlands, Canary Island).

RESEARCH AWARDS AND ACHIEVEMENTS

OVERSEAS VISITS BY STAFF & STUDENTS


The Division Plant Pathology at the University of Pretoria was recently audited by the South African National Accreditation System (SANAS) and subsequently qualified for ISO 17025 accreditation. This is a first for an agricultural research laboratory at a tertiary institution. SANAS is internationally recognised and is the national accreditation body of South Africa, which accredits laboratories to the relevant national and international standards. ISO 17025 is an international standard for testing and calibration laboratories where they have to demonstrate that they operate a quality system, are technically competent and are able to generate true and accurate test results.

Laboratories are assessed every six months to ensure that compliance to the standard is upheld. The Plant Pathology Laboratories are situated within UP’s Faculty of Natural and Agricultural Sciences. The scope of this accreditation includes testing a variety of samples for the presence of microbes, monitoring of coliforms in packhouse water, detection of food borne pathogens in packhouses and on fruit surfaces, testing of biocontrol products and PCR-based detection and fingerprinting of a wide range of plant pathogens. A case in point to illustrate the enormous value of this accreditation to the industry is the use of PCR to test export consignments of citrus for a disease called black spot. During the 2001 season, citrus growers in the Letsitele Valley lost millions of Rands due to rejections. This rapid, accurate technique can be used to test fruit prior to transportation to the harbours. This accreditation now allows the Plant Pathology Laboratories to do diagnostic work for the fruit industries, which can be upheld in an international court of law should a dispute arise.

PLANT PATHOLOGY RECEIVES ISO 17025

Dr Mike Peet (left) Prof Lise Korsten and Prof Callie Pistorius (Rector)


Water Biotechnology: PROF T E CLOETE The Water Biotechnology Group focused their research on the passive treatment of acid mine drainage, microbial community diversity studies in activated sludge, and on solar pasteurisation of water supplies in rural areas. A new dimension was added with the three PhD students in Water Resource Management focusing on a multi-criteria decision making model for integrated catchment management in the one instance, a national strategy for monitoring the microbial quality of water supplies in the other and the third project revolving around the development of a national strategy for water supply in rural areas. Significant advances have been made in the development of a passive treatment system for acid mine drainage. The group working on activated sludge population dynamics, indicated for the first time, that the exopolymers produced by bacteria in activated sludge contained phosphorus and could play a major role in biological phosphorus removal from waste water. Research on solar pasteurisation indicated that this technique could be used successfully for treating groundwater to meet potable standards, hence providing an ideal cost effective method for treating contaminated water in rural areas. Forest/tree pathology: COUTINHO TA Host-pathogen interactions, ecology and biology of important tree pathogens.

White rust of Sunflower: AVELING TAS, VILJOEN A White rust is considered the most damaging sunflower disease in South Africa, and is caused by the obligate parasite Albugo tragopogonis. Research on the disease is difficult, since no greenhouse inoculation techniques are available. The development of such a technique will assist in the study of control methods which will include chemical control.

Panama disease of bananas: VILJOEN A Panama disease is considered to be one of the most damaging plant diseases in the world. In South Africa, the disease is particularly severe in the Kiepersol and Southern KwaZulu -Natal area. Since no effective method of control is available, it is important to develop an integrated disease management program to limit the effect of the disease in the field. Such a programme integrates plant disease resistance, biological control, general plant health and disease management. It is also necessary to analyse the pathogen population, and to determine the spread of the fungus. Plantation tree pathology COUTINHO TA Monitoring of the health status of trees. Identification of tree pathogens and disease diagnosis (through monitoring and submission of samples).

RESEARCH PROJECTS


Postharvest Pathology KORSTEN L, SWART G Pre and postharvest factory that influence postharvest quality of fruits and vegetables are studied to ensure sustained market access through quality assurance. Postharvest pathogens that infect fruits and vegetables pre or postharvestly are studied in terms of ecology, biology and control. Integrated disease management: KOTZÉ JM, KORSTEN L, WEHNER FC, LABUSCHAGNE N, Alternative disease control strategies as part of integrated disease management systems focus on the use of biological and other natural control options. The reduced use of agrochemicals coupled with more environmentally friendly ways to control diseases and enhance plant growth, forms an integral part of IDM systems. Hydroponic pathology: LABUSCHAGNE N The main focus is the etiology and control of Pythium wilt and root rot in hydroponic systems. A disease control strategy is being developed for the different types of systems. A range of Pythium isolates have been isolated from the main hydroponic crops. These isolates are now being identified and screened for pathogenicity. Production of disease tolerant forest trees: COUTINHO TA Identification of new pathogens; studies on the biology and population genetics of important pathogens; development and

implementation techniques for rapidly screening species and clones for resistance to disease; disease surveys; investigation into management strategies including cultural, chemical and biological control. Nitrogen Fixation: JAFTHA JB, STEYN PL, STRYDOM B Leguminnous plants and their rhizobial symbionts are investigated to increase the application of Biological Nitrogen Fixation in South African agriculture. Rhizobia from a wide range of plant hosts are characterised in an effort to determine the diversity of the bacterial symbionts and possibly identify inoculant species for agricultural application. Biofilm physiology: BRÖZEL VS, THERON J, VENTER SN The bulk of bacteria occuring in the environment grow at surfaces, forming biofilms. Bacteria growing in biofilms display a unique phenotype distinct from broth or agar cultured ce lls. The behaviour of Pseudomonas aeruginosa and Bacillus cereus in biofilms is under investigation by proteomic (2D electrophoretorgrams of proteins), as well as genetic analysis. The regulation and function of a number of regulatory elements of P. aeruginosa affected during growth as a biofilm are being studied. The growth, activity and survival of pathogenic bacteria in drinking water biofilms is also under investigation. Molecular biology and virology: NEL LH, THERON J, VILJOEN G, INJAIRU RM, DUNGU-KIMBENGA B Gene manipulation technology is used in the study of aspects of gene expression and


protein function in a variety of micro-organisms, including a number of important viruses. Molecular genetic analysis is also implemented in the diagnostics and taxonomy of various important groups of viruses, bacteria and fungi. Protozoan parasites: VENTER SN The methods for the concentration, detection and typing of Cryptosporidium and microsporidia in various types of water samples are being developed. Bacterial pathogens in water: BRÖZEL VS, THERON J, VENTER SN The research focus entails the development of detection and identification methods, as well as a study of the fate (survival) of water-borne pathogens. Detection, typing and identification methods for Campylobacter and Vibrio cholerae in biofilms as well as surface and drinking water sources are developed. The prevalence of bacterial pathogens in biofilms associated with drinking water is investigated, as is the survival of pathogens in fresh water sediments. Biochemical aspects of the quality and health properties of tea: APOSTOLIDES Z, LOUW AI, GASPAR ARM, LABUSCHAGNE N DNA primers are used to identify tea cultivars of different qualities, and the role of polyphenols in conferring quality and health aspects on tea is investigated.

Environmental biotechnology: JOOSTE A, CLOETE TE, ASHTON P, JOOSTE P, VAN BUUREN J The use of micro-organisms to inhibit pollution is researched. Emphasis is placed on water pollution with special reference to waste water, industrial waste water, industrial effluent cooling water and water supply. Molecular systematics of micro-organisms: WINGFIELD BD, COUTINHO TA, VAN DER WALT JP Description and identification of micro-organisms using molecular techniques. Description of populations at the molecular level. Food safety and quality assurance for hydroponic vegetable production systems and minimal processing plants: KORSTEN L, DU PLOOY W, HALL A The development and implementation of a quality assurance system for the production and minimal processing of Primary agriculture and fruits and vegetables. Biocontrol of plant diseases: KORSTEN L, WEHNER FC, LABUSCHAGNE N Development of natural disease control products such as natural plant extracts and microorganisms that can control the most important plant diseases on a commercial scale.


Soilborne diseases: LABUSCHAGNE N, REGNIER T, APOSTOLIDES Z, HUGUES B Biochemical resistance mechanisms against soilborne pathogens are being investigated in citrus rootstocks. The yam nematode is being studied pertaining to aspects such as pathogenicity, genetic variability and rapid resistance screening.

Citrus and Subtropical research KORSTEN L, WEHNER FC, LABUSCHAGNE N, SWART G The most important diseases of citrus and mango are studied and include citrus greening, blackspot and root rot. Mango research focus mainly on treeding back, black spot anthracnose and soft brown rot. Forest biotechnology: COUTINHO TA, PREISIG O Development of the technology to mass propagate clonal material and to produce disease free and pest resistant transgenic trees.

Sustainable insect management: CHOWN SL, COUTINHO TA, CREWE RM, FLOYD R, GOVENDER P, KORSTEN L, LIEUTIER F, MCGEOCH MA, MUTITU E, OBERHOLSTER A-M, ROBINSON D, SCHOEMAN AS, SCHOLTZ CH, VILJOEN A, WINGFIELD The scientific integration of agriculture and conservation goals using principles of integrated pest management. A survey and study of the plant pathogens reducing yields of indigenous food crops, and the transmission and control of these pathogens: AVELING TAS, LABUSCHAGNE N Light and electron microscopy of host/ pathogen interactions; seed pathology; seed health testing; control of seed-borne diseases; disease survey.


1. ADANDONON A, AVELING TAS, LABUSCHAGNE N: 2001. Pythium Rhizoctonia complex associated with damping-off of Cowpea in South Africa. African Plant Protection, 7, pp 17-25

2. BARTIE C, VENTER SN, NEL LH: 2001. Evaluation of detection methods for Legionella species using seeded water samples. Water SA, 27, pp 523-527

3. BASTOS AD, HAYDON D, FORSBERG R, KNOWLES NJ, ANDERSON EC, BENGIS RG, NEL LH, THOMSON GR: 2001. Genetic heterogeneity of SAT-1 type foot-and-mouth disease viruses in southern Africa. Archives of Virology, 146, pp 1-15

4. BINGHAM J, JAVANGE S, SABETA C, WANDELER AI, NEL LH: 2001 Report of isolations of unusual lyssaviruses (rabies and Mokola virus) identified retrospectively from Zimbabwe. Journal of the South African Veterinary Association 72 (2): 92-94

5. VAN DER WALT JM, NEL LH, HOELZEL, AR: 2001 Characterization of major histocompatibility complex DRB diversity in the endemic South African antelope Damaliscus pygargus: a comparison in two subspecies with different demographic histories. Molecular Ecology 10: 1679-1688

6. BELAY TA, CLAASSENS AS, WEHNER FC, DE BEER JM: 2001. Influence of residual manure on selected nutrient elements and microbial composition of soil under long-term crop rotation. Plant and Soil, 18, pp 1-6

7. BOTHA FS, BOTHA SJ, KROON J, STEYN PL: 2001. Caries prediction factors in children with primary dentition. South African Dental Journal, 56, pp 348-352

8. BRITZ H, COUTINHO TA, GORDON TJ, WINGFIELD MJ: 2001. Characterisation of the pitch canker fungus, Fusarium circinatum from Mexico. South African Journal of Botany, 67, pp 609-614

9. BURGESS TI, MASEKO B, COUTINHO TA, WINGFIELD MJ: 2001. First report of Phytophthora nicotianae associated with Eucalyptus die-back in South Africa. Plant Pathology, 50, p 413

10. CLOETE TE, DE BRUYN EE: 2001. The effect of culture media on antigenic expression in sulphate reducing bacteria. Current Microbiology, 42, pp 305-309

11. CLOETE TE, JACOBS L: 2001. Surfactants and the attachment of Pseudomonas aeruginosa to 3CR12 stainless steel and glass. Water SA, 27, pp 21-26

12. CLOETE TE, OOSTHUIZEN DJ: 2001. SEM-EDA for determining the phosphorus content in activated sludge EPS. Water Science and Technology, 43, pp 105-112

13. CLOETE TE, OOSTHUIZEN DJ: 2001. The role of extracellular exopolymers in the removal of phosphorus from activated sludge. Water Research, 35, pp 3595-3598

14. DE JAGER ES, WEHNER FC, KORSTEN L: 2001. Microbial ecology of the mango phylloplane. Microbial Ecology, 42, pp 201-207

15. DE WET J, WINGFIELD MJ, COUTINHO TA, WINGFIELD BD: 2001. Characterisation of the 'C' morphotype of the pine pathogen Sphaeropsis sapinea. Forest Ecology and Management, 5543, pp 1-8

16. FLETT BC, MCLAREN NW, WEHNER FC: 2001. Incidence of Stenocarpella and Fusarium

PUBLICATIONS IN SCIENCE CITATION INDEX JOURNALS


ear rots in corn crop rotation systems. Plant Disease, 85, pp 92-94 17. GRIMBEEK E, BENTLEY S, VILJOEN A: 2001. First occurrence of Panama disease in two

banana growing areas of South Africa. Plant Disease, 85, p 1211 18. HEATH L, VAN RENSBURG HG, VOSLOO W, NEL LH: 2001. The 3A non-structural-

protein coding region of the southern African SAT type isolates differs from that of other foot-and-mouth disease viruses. Onderstepoort Journal of Veterinary Research, 68, pp 253-262

19. HENNING BJ, SNYMAN HG, AVELING TAS: 2001. Plant-soil interactions of sludge-borne heavy metals and the effect on maize (Zea mays L.) seedling growth. Water SA, 27, pp 71-77

20. JACOBS K, WINGFIELD MJ, JACOBS A, WINGFIELD BD: 2001. A taxonomic re-evaluation of Phialocephala phycomyces. Canadian Journal of Botany, 79, pp 110-117

21. JANSEN VAN RENSBURG C, JANSEN VAN RENSBURG JC, LABUSCHAGNE N, NEMEC S: 2001. Occurrence of Fusarium-produced naphthazarins in citrus trees and sensitivity to isomarticin in relation to citrus blight. Plant Pathology, 50, pp 258-265

22. KORF HJG, SCHUTTE GC, KOTZE JM: 2001. Effect of packhouse procedures on the viability of Phyllosticta citricarpa, anamorph of the citrus black spot pathogen. African Plant Protection 7(2)

23. MEYER L, SLIPPERS B, KORSTEN L, KOTZE JM: 2001. Two distinct Guignardia species associated with citrus in South Africa. South African Journal of Science, 97, pp 191-194

24. OOSTHUIZEN MC, STEYN B, LINDSAY DL, BRÖZEL VS, AND VON HOLY A: 2001 Novel method for the proteomic investigation of a dairy-associated Bacillus cereus biofilm FEMS Microbiol. Letts 194, 47 – 51

25. ROUX J, COUTINHO TA, BYABASHAIJA DM, WINGFIELD MJ: 2001. Diseases of plantation Eucaluptus in Uganda. South African Journal of Science, 97, pp 16-18

26. ROUX J, STEENKAMP ET, MARASAS WFO, WINGFIELD MJ, WINGFIELD BD: 2001. Characterisation of Fusarium graminearum from Acacia and Eucalyptus using ß-tubulin and histone gene sequences. Mycologia, 93, pp 704-711

27. SLIPPERS B, WINGFIELD MJ, COUTINHO TA, WINGFIELD BD: 2001. Population structure and possible origin of Amylostereum areolatum in South Africa. Plant Pathology, 50, pp 206-210

28. SMITH H, CROUS PW, WINGFIELD MJ, COUTINHO TA, WINGFIELD BD: 2001. Botryosphaeria eucalyptorum sp. Nov., a new species in the B. dothidea-complex on Eucalyptus in South Africa. Mycologia, 93, pp 277-285

29. STEYN B, OOSTHUIZEN, MC, MACDONALD RM, THERON J AND BRÖZEL VS: 2001 The use of glass wool as an attachment surface for studying phenotypic changes in Pseudomonas aeruginosa biofilms by two dimensional gel electrophoresis. Proteomics 1, 871 – 879

30. THERON J, MORAR D, DU PREEZ M, BRÖZEL VS, VENTER SN: 2001. A sensitive seminested PCR method for the detection of Shigella in spiked environmental water samples. Water Research, 35, pp 869-874

31. VAN HEERDEN J, EHLERS MM, CLOETE TE: 2001. Biolog for the determination of microbial diversity in activated sludge systems. Water Science and Technology, 43, pp 83-90

32. VAN HEERDEN S, WINGFIELD MJ: 2001. Genetic diversity of isolates in South Africa. Mycological Research, 105(1), pp 94-99

33. VENTER M, WINGFIELD MJ, COUTINHO TA, WINGFIELD BD: 2001. Molecular characterisation of Endothia gyrosa isolates from Eucalyptus in South African and Australia. Plant Pathology, 50, pp 211-217


1. BRÖZEL VS: 2001. Viability evaluation of a dairy-associated Bacillus cereus following sanitizer treatment. In:

Proceedings of the 40th Annual Conference of the Microscopy Society of Southern Africa: Microscopy Society of Southern Africa, Johannesburg, South Africa, p 45.

2. LE ROUX JJ, HALL AN, JAFTHA JB, STEYN PL: 2001. A Novel Bacillus species isolated from Leguminous root-nodules. In: Proceedings of the 40th

Annual Conference of the Microscopy Society of Southern Africa: Microscopy Society of Southern Africa, pp 43-43.

3. MACDONALD R, BOTHA AJ, THERON J, BRÖZEL VS: 2001. Use of low vacuum scanning electron microscopy in determining biofilm structure. In: Proceedings of the 40th Annual Conference of the Microscopy Society of Southern Africa: Microscopy Society of Southern Africa, p 51.

4. MACDONALD R, THERON J, BRÖZEL VS: 2001. The role of alternative sigma factor rpoS in determining the biofilms structure of Pseudomonas aeruginosa. In: Proceedings of the 40th Annual Conference of the Microscopy Society of Southern Africa: Microscopy Society of Southern Africa, p 50.

1. BARTIE C, VENTER SN, NEL LH: 2001. Legionella detection from South African cooling

water systems. In: Legionella, ASM Press, p 56. 2. CLOETE TE, ALLISON PJ, POULTON WIJ: 2001. The detection and Mitigation of MIC. In:

Microbiologically Influenced Corrosion, Volume 2 pp 11.15 - 11.20. 3. LABUSCHAGNE N: 2001. Guide to Hydroponic Vegetable Production. In: Agricultural

Research Council. 4. NEL LH: 2001. “Animal viruses pathogenic for humans.” In: Encyclopedia of Life

Sciences/www.els.net. Atlas, R. (Editor-in-chief). Nature Publishing Group ? 2001 Macmillan Publishers Ltd. Reg.No. 785998.

5. ATLAS RM, MICHEL A, NEL LH: 2001. “Tuberculosis in African wildlife and other infectious diseases of African lions.” A3371 In: Encyclopedia of Life Sciences/www.els.net. Atlas, R.M. (Editor-in-chief). Nature Publishing Group ? 2001 Macmillan Publishers Ltd. Reg.No.785998 England.

CONTRIBUTIONS IN PUBLISHED REFEREED CONFERENCE PROCEEDINGS

CHAPTERS IN REFEREED SPECIALIST BOOKS


Keynote addresses 1. CLOETE TE: The 6th Annual Water Africa Conference. Keynote address. The Cholera

pandemic in South Africa. Johannesburg, South Africa, September 2001 2. CLOETE TE: Sewage and effluent summit. Keynote address. Solar disinfection of potable water.

Johannesburg, South Africa, September 2001. 3. KORSTEN L: Challenges and opportunities for subtropical farmers. Sustainable Agriculture

Study Group meeting, Malelane, South Africa, 19 April 2001 4. KORSTEN L: Avogreen, the road to commercialisation. Avocado growers study group meetings,

Levubu, 18 January 2001, Nelspruit, 16 January 2001, Hazyview and 17 January 2001, Wartburg 5. NEL LH: Emerging pathogens. 2nd Molecular and cell biology symposium, Johannesburg, South

Africa May 2001 Invited Paper 1. AVELING TAS: Susceptibility of cowpea seeds with different seed coat colours to damping-off.

39th South African Society for Plant Pathology, Nelspruit, South Africa, 2001 2. CLOETE TE: Conference of Technology Teachers. Guest Speaker. Creativity. Johannesburg,

South Africa, September 2001 3. BELAY TA, CLAASSENS AS, WEHNER FC: Influence of residual manure on maize (Zea mays

l.) yield, nutrient dynamics and microbial properties of soil under long-term crop rotation. Joint Congress 2001: Soil Science Society of South Africa, South African Society of Crop Production and Southern African Weed Science Society, Pretoria, South Africa, 16-18 January 2001

4. LABUSCHAGNE N: Disease management of greenhouse crops: the role of technology. International Plant Propagators Society 4th Annual Conference, Pretoria, South Africa, 8-9 May 2001

5. LE ROUX JJ, JAFTHA JB, STEYN B: A novel Bacillus species isolated from legumincus root nodules. Microscopy Society of Southern Africa, Johannesburg, South Africa, 5-7 December 2001

6. KORSTEN L: Role of agricultural regulatory services in ensuring food safety. Minister Indaba 2001: Ensuring food security and food safety in South Africa through an effective regulatory environment, Kyalami, South Africa, 5 October 2001

7. KORSTEN, L: 2001 Bioproducts and control – Fact or Fiction? “From the Seed to the Plate – Global influences on the fresh produce industry” Eskom Conference Centre, Midrand 22 – 23 August 2001.

8. KORSTEN, L: Commercialisation aspects of biocontrol products. Series of presentations for avocado growers at study group meetings in Hazyview, Tzaneen and Levubu 29 – 31 Augustus 2001.

9. KORSTEN, L: Food safety and quality. SAMGA Marketing Day, Greenway Woods Golf Resort, White River. 5 September 2001.

10. KORSTEN, L: Food Safety, GAP and HACCP. Fresh Produce Management Workshop. Fresh

NATIONAL CONFERENCE CONTRIBUTIONS


Produce Management Training Initiative. Overberg Converence Centre, Grabouw. 17 August 2001.

11. KORSTEN, L: Food Safety: A practical approach to ensure good quality and safe product. African Women Agribusiness Network Development (AWAND) Workshop. Table Bay Hotel, Cape Town. 16 August 2001.

Papers 1. CLOETE TE: Sewage and effluent summit, Solar disinfection of potable water. Johannesburg,

September 2001. 2. CLOETE TE: The 6th Annual Water Africa Conference. The Cholera pandemic in South Africa.

Johannesburg, South Africa, September 2001. 3. SLIPPERS B, COUTINHO TA, WINGFIELD BD, CROUS PW, WINGFIELD MJ: Taxonomy

and phylogeny of Botryosphaeria spp. Southern African Society for Plant Pathology, 21-24 January 2001

4. HUNTER GC, ROUX J, COUTINHO TA, WINGFIELD MJ, CROUS PW: Mycosphaerella species causing Mycosphaerella leaf blotch (MLB) on Eucalyptus in South Africa. Southern African Society for Plant Pahtology, 21-24 January 2001.

5. MASEKO BOZ, BURGESS T, COUTINHO TA, WINGFIELD MJ: First report of Phytophthora nicotianae associated with Eucalyptus die-back in South Africa. Southern African Society for Plant Pathology, 21-24 January 2001

6. JACOBS R, SLIPPERS B, KORSTEN L: Botryosphaeria species from mango. 39th Annual Congress of the South African Society for Plant Pathologty, Witrivier, South Africa, 21-24 January 2001

7. GROBLER L, JACOBS R, KORSTEN L: Die etiologie van terugsterwing op mangobome. South African Mango Research Symposium, Tzaneen, South Africa, 21 June 2001

8. KORSTEN L: An overview of mango pathological research at University of Pretoria. South African Mango Research Symposium, Tzaneen, South Africa, 21 June 2001

9. KORSTEN L, DU PLOOY GW: Total quality management systems for food production in South Africa. 39th Annual Congress of the South African Society for Plant Pathology, Witriver, South Africa, 21-24 January 2001

10. SWART GM, KORSTEN L: Detection of citrus greening disease from different sources and distinguishing between leaf symptoms using PCR. 39th Annual Congress of the South African Society for Plant Pathology, Witrivier, South Africa, 21-24 January 2001

11. VISMER HF, MARASAS WFO, COUTINHO TA: The effects of fenpropathrin and other compounds on the growth and presence of mites in fungal cultures. Southern African Society for Plant Pathology, 21-24 January 2001

12. SITHOLE H, GOVENDER P, VILJOEN A: Integrated pest management of the banana weevil, Cosmopolites sordidus. Proceedings of the 13th Entomological Congress, Pietermaritzburg, South Africa, 2-5 Jul 2001

13. VILJOEN A: Integrated pest management of the banana weevil, Cosmopolitus sordidus. African Entomological Society Meeting, Pietermaritzburg, South Africa, July 2001

14. GOOSEN C, THERON J, NTSALA M, MAREE FF, OLCKERS A, BOTHA SJ, FIERENS C, VAN DER MERWE SW: Evaluation of a novel hemi-nested PCR assay based on the phosphoglucosamine mutase gene for detection of Helicobacter pylori. 35th Scientific Meeting of the International Association for Dental Research (SA Div), Broederstroom, South Africa, 29-30 August 2001

15. GOOSEN C, THERON J, NTSALA M, MAREE FF, OLCKERS A, BOTHA SVJ, VAN DER


MERWE SW: Evaluation of a novel hemi-nested PCR assay based on the phosphoglucosamine mutase gene for detection of Helicobacter pylori in saliva and dental plaque. The South African Gastroenterology Society Congress, Durban, August 2001

16. MEYER L, KORSTEN L, SLIPPERS B, WINGFIELD MJ: Phyllosticta species on citrus. 39th Annual Congress of the South African Society for Plant Pathology, Witrivier, South Africa, 21-24 January 2001

17. OBAGWU J, KORSTEN L, WEHNER FC, PAUL I: Inhibitory action of natural epiphytes isolated from citrus fruit surface against Penicillium digitatum Sacc. The cause of citrus green mould. 39th Annual Congress of the South African Society for Plant Pathology, Witrivier, South Africa, 21-24 January 2001

18. PAUL I, OBAGWU J, KORSTEN L: Alternative control of green mould caused by Penicillium digitatum Sacc. on valencia oranges. 39th Annual Congress of the South African Society for Plant Pathology, Witrivier, South Africa, 21-24 January 2001

19. SLIPPERS B, WINGFIELD MJ, KORSTEN L: Soft brown rot, tree die -back and other diseases caused by the same pathogen. South African Mango Research Symposium, Tzaneen, South Africa, 21 June 2001

20. SWART GM, KORSTEN L: Anthracnose on mango- an overview. South African Mango Research Symposium, Tzaneen, South Africa, 21 June 2001

21. SWART GM, KORSTEN L: An investigation into chemical resistance on mango - is it a problem? South African Mango Research Symposium, Tzaneen, South Africa, 21 June 2001

22. JACOBS R, SLIPPERS B, WINGFIELD M, KORSTEN L: 2001. Soft brown rot, tree die-back and other diseases caused by the same pathogen. South African Mango Research Symposium, 21 June 2001, Tzaneen. Paper.

23. SILIMELA M, KORSTEN L: 2001. Evaluating plastic caps and biocontrol in the pre-harvest environment. South African Mango Research Symposium, 21 June 2001, Tzaneen. Paper.

24. GOVENDER V, KORSTEN L: 2001. Evaluating biological control for mango postharvest diseases control. South African Mango Research Symposium, 21 June 2001, Tzaneen. Paper.

25. DE WET J, KORSTEN L: 2001. Evaluering van chemikalieë in die na -oes omgewing vir die beheer van na-oes siektes. South African Mango Research Symposium, 21 June 2001, Tzaneen. Paper.

Posters 1. LABUSCHAGNE N, VERWEY F: Efficacy of phosphorate fungicides for control of Pythium root

rot and wilting disease in hydroponically grown lettuce. 39th Congress, South Africa Society of Plant Pathology, Witrivier, South Africa, January 2001

2. GULL C, LABUSCHAGNE N, BOTHA W: Pathogenicity of different Pythium isolates on butter lettuce in hydroponic systems under greenhouse conditions. 39th Congress, SA Society of Plant Pathology, Witrivier, South Africa, January 2001

3. BRÖZEL VS: Growth of Bacillus cereus and Pseudomonas fluorescens binary biofilms and response to a chlorine dioxide-containing sanitizer in a model flow system. 16th Biennial Congress of the South African Association for Food Science and Technology. Durban, South Africa, 10-12 September 2001.

4. PETA ME, LINDSAY D, BRÖZEL VS, VON HOLY, A: Response of food spoilage Bacillus spp. to three acid based sanitizers. 16th Biennial Congress of the South African Association for Food Science and Technology. Durban, South Africa, 10-12 September 2001.

5. PETA ME, LINDSAY D, BRÖZEL VS, VON HOLY, A: Differential effect of organic matter on the efficacies of four sanitizer types. 16th Biennial Congress of the South African Association for


Food Science and Technology. Durban, South Africa, 10-12 September 2001. 6. MACDONALD R, BOTHA A, THERON J, BRÖZEL VS: Use of low-vacume scanning electron

microscopy in determining biofilm structure. Conference of the Microscopy Society of South Africa. Johannesburg, South Africa, 5-7 December.

7. MACDONALD R, THERON J, BRÖZEL VS: The role of the alterna tive sigma factor rpoS in determining the biofilm structure of Pseudomonas aeruginosa. Conference of the Microscopy Society of South Africa, Johannesburg, South Africa, 5-7 December.

8. PETA ME, LINDSAY D, BRÖZEL VS, VON HOLY, A: Viability evaluation of a dairy assocation Bacillus cereus following sanitizer treatment. Conference of the Microscopy Society of South Africa, Johannesburg, South Africa, 5-7 December.

9. BANDOUNAS T, VILJOEN A, AVELING TAS: Ethograph of Albugo tragopogonis on sunflower. 39th South African Society for Plant Pathology, Nelspruit, South Africa.

10. VILJOEN A, SURRIDGE K, WEHNER FC: Distribution of banana leaf pathogens in South Africa in 2000. 39th South African Society for Plant Pathology, Nelspruit, South Africa.

11. VILJOEN A, SURRIDGE K, WEHNER FC: Fungi associated with banana leaves in South Africa. 39th South African Society for Plant Pathology, Nelspruit, South Africa.

12. KRITZINGER Q, AVELING TAS, MARASAS WF, SHEPARD GS, LEGGOTT NL: Mycotoxins associated with cowpea seeds. 39th South African Society for Plant Pathology, Nelspruit, South Africa.

13. VAN DEN BERG N, AVELING TAS: Evaluation of six fungicides for controlling Altemaria cassiae on cowpea seeds. 39th South African Society for Plant Pathology, Nelspruit, South Africa.

14. LINDSAY, D., BRÖZEL, V.S., MOSTERT, J.F. AND VON HOLY, A. Growth of Bacillus cereus and Pseudomonas fluorescens binary biofilms and response to a chlorine dioxide-containing sanitizer in a model flow system. 16th Biennial Congress of the South African Association for Food Science and Technology, 10 – 12 September, 2001, Durban, South Africa.

15. GRIMBEEK E, VILJOEN A, COUTINHO TA: The distribution and spread of Fusarium wilt of bananas in South Africa. 39th South African Society for Plant Pathology, Nelspruit, South Africa , 2001

16. COUTINHO TE, PREISIG O, MERGAERT J, CNOCKAERT MC, RIEDEL K-H, SWINGS J, WINGFIELD MJ: A new bacterial disease of Eucalyptus species, hybrids and clones in South Africa. Southern African Society for Plant Pathology, 21-24 January 2001


Session chairperson 1. AVELING TAS: Recent Developments in Crop Pre-and Post harvest Pest Management

Practices in Africa. Second International Workshop of African Network of Research on Bruchids, Cotonou, Benin, 2001

Invited Paper 1. AVELING TAS, POWELL AA: Effect of storage potential and seed coat pigmentation on

susceptibility of cowpeas to pre-emergence damping-off. 26th Congress of the International Seed Testing Association, Angers, France, 14-22 June 2001

2. AVELING TAS, ADANDONON A, KRITZINGER Q, PAKELA YP, SMITH JE, VAN DEN BERG N: Research on cowpea diseases in South Africa. Second International Workshop of African Network of Research on Bruchids: Recent Developments in Crop Pre-and Post-harvest Pest Management Practices in Africa, Cotonou, Benin, 12-17 November 2001

3. AVELING TAS: Participation in discussions. The International Society of Plant Pathology-Sponsored Instructional Technology Symposium 2001, 2001

4. AVELING TAS: International Workshop. International Workshop on Seed Health Testing, Taipei, Taiwan, 3-8 December 2001

5. OOSTHUIZEN M, STEYN B, BRÖZEL VS, LINDSAY D, VON HOLY A: Dairy-association Bacillus cereus growing as a biofilm has a distinct proteome. 88th Annual Meeting of the International Association for Food Protction, Minnesota, USA, 5-8 August 2001

6. CLOETE TE: The chemical control of biofouling in industrial water systems. International Conference on Biofouling and Materials, Frankfurt am Main/DECHEMA House, June 2001

7. CLOETE TE: Resistance mechanisms of microorganisms to antimicrobial compounds. Second International Symposium on Disinfection and Hygiene, Wageningen, October 2001

Papers 1. CLOETE TE, OOSTHUIZEN DJ: SEM-EDS for determining the phosphorus content in

activated sludge EPS. IWA, Specialist Conference on Microbial Population Dynamics, Rome, Italy, June 2001

2. CLOETE TE, OOSTHUIZEN DJ: Phosphorus removal capacity of MLSS and MLVSS fractions of five activated sludge plants. IWA, Specialist Conference on Mirobial Population Dynamics, Rome, Italy, June 2001

3. NEL LH: Mokola virus: A brief review of the status quo. 6th Conference of the southern and eastern African rabies group. Lilongwe, Malawi, June 2001.

4. KRITZINGER Q, AVELING TAS, MARASAS WF: Effect of essential plant oils on the growth of storage fungi associated with cowpea seeds. 26th Congress of the International Seed Testing Association, Angers, France, 14-22 June 2001

5. ADANDONON A, AVELING TAS, LABUSCHAGNE N, AHOHUENDO B: Epidemiology and biological control of the causal agent of damping-off and stem rot of cowpea in the QUEME va lley, Benin. Second International workshop of African Network of Research on

INTERNATIONAL CONFERENCE CONTRIBUTIONS


Bruchids, Cotonou, Benin, 12-17 Nov 2001 6. AVELING TAS, ADANDONON A, KRITZINGER Q: Research developments on cowpea

diseases in South Africa. 13th Biennial Conference of the Australasian Plant Pathology Society, Cairns, Australia, 24-27 September 2001

7. KRITZINGER Q, AVELING TAS, MARASAS WFO: Contamination of cowpea (Vigna unguiculata (L.) Walp) seed by storage fungi and mycotoxins. British Mycological Society International Symposium on Bioactive Fungal Metabolites - Impact and Exploitation, Swansea Wales, 22-27 April 2001

8. HAWUMBA JF, THERON J, BRÖZEL VS: Thermophilic protease-producing Bacillus from Buranga hot spring in Western Uganda. Ninth symposium of the Natural Products Research Network for Eastern and Central Africa, Nairobi, Kenya, 27-31 August 2001

9. PETA ME, LINDSAY D, BRÖZEL VS, VON HOLY A: Response of Food spoilage Bacillus spp. to three acid-based sanitizers. 88th Annual Meeting of the International Association for Food Protection, Minnesota, USA, 5-8 August 2001

10. VAN DER WAALS (SMITH) JE, KORSTEN L, DENNER FDN: Accuracy of spore dispersal module in the potato early blight forecaster, PLANT-Plus, in South Africa. Eighth International Workshop on Plant Disease Epidemiology, Ouro Preto, Brazil, 6 - 11 May 2001

Posters 1. BRÖZEL VS: 101st General Meeting of the American Society for Microbiology, Orlando, FL,

USA, 2. BRÖZEL VS: 88th Annual Meeting of the International Association for Food Protection,

Minnesota, USA, 5-8 August 2001 3. BRÖZEL VS: Pseudomonas 2001, Brussels, Belgium, 16-21 September 2001 4. BROZEL VS: Ninth symposium of the Natural Products Research Network for Eastern and

Central Africa, 27-31 August, Nairobi, Kenya 5. VENTER SN: ASM 101st General Meeting, Orlando, USA 6. CLOETE TE: International Water Association (IWA) Specialist Conference on Microbial

Population Dynamics, Rome, Italy 7. CLOETE TE: IWA World Congress, Berlin, Germany 8. COUTINHO TA: Annual Meeting of the Mycological Society of America, Vermont, USA 9. COUTINHO TA: Annual Meeting of the Phytopathological Society of America, Salt Lake City,

Utah, USA


MSc – Microbiology ERASMUS J: Cloning and expresion of Clostridium type C and D neurotoxim genes. (Prof LH Nel) ESPACH A: Towards DNA vaccines for Rift Valley Fever. (Prof LH Nel) HEATH L: Characterisation of structural protein - encoding genes of FMD virus SAT3. (Prof LH Nel) MALHERBE S: Biological pretreatment of lignocellulosic carbon sources for use in the passive treatment on mine water. (Prof TE Cloete) NTSUDISANE B: Biomass and its role in biological phosphorus removal. (Prof TE Cloete) OOSTHUIZEN DJ: Bioaugmentation of activated sludge for Enhanced Biological phosphorus removal. (Prof TE Cloete) STIPINOVICH C: Genomic sequencing and characterization of Lumpy Skin Disease virus. (Prof LH Nel) VAN HEERDEN J: The determination of microbial species diversity and evenness inactivated sludge systems. (Prof TE Cloete) BENGURIC D: Anti-idiotype antibodies and phage displayed peptides as antigenic mimics of a Mycoplasma capricolum subsp. Capripneumoniae epitope. (Prof L H Nel) ORTIZ V G: The molecular evaluation of transovarial transmission of the Uruguayan vaccine of Babesia bovis and Babesia bigeni. (Prof L H Nel) MSc or M Inst Agrar – Plant Pathology MATLALA AK: Epidemiology and detection of erwinias on potatoes.. (Prof L Korsten) GANTSHO N: Comparison of Xanthomonas campestris pv mangiferaeindicae from mango producing areas in South Africa. (Prof L Korsten) VENTER M: Studies on Endothia gyrosa. (Prof TA Coutinho, Prof MJ Wingfield) AURET EE: Control strategies for citrus postharvest disease. (Prof L Korsten) MSc – Water Resource Management (Course Work) BEUKES H NEMATO H GUMBO J SCHEEPERS N MASOABI D POSTHIMUS C PhD – Microbiology SLAGER-BASTOS A: Molecular epidemiology of foot and mouth disease virus in Southern Africa. (Prof LH Nel) JACOBS A: Re-evaluation of the genus Phialocephala. (Prof MJ Wingfield) STEENKAMP ET: Molecular taxonomy studies of species in the Gibberella fujikuroi complex. (Prof BD Wingfield) VAN RENSBURG HG: Development of new generation Foot and Mouth Disease Virus vaccines. (Prof L H Nel) PhD – Plant Pathology KWEREPE BC: Integrated pest management of Meloidogyne incognita on bambara groundnut Vigna subterranea. (Dr N Labuschagne)

MSc AND PhD DEGREES AWARDED


MSc-Students Beukes, H Espach, A Gantsho, N Goosen, C Gumbo, J Begashaw, LM Nuthelezi, B Chale-Matsau, B Hechter, W Hlupheka, P Hunter, GC Juckers, E Verwey, F

Auret, EE Havenga, W Heath, LE Cronje, C Scheepers, N Stipinovich, C Van Heerden, J Wagner, A Benguric, D De Jager, ES Bandounas, T Dilima, N Grimbeek, E

Louw, K Mashau, F Meiring, MS Mienie, NJJ Masoabi, T D Matlala, AK Mnisi, S Mphahlele, M Mphalele, MP Nakabonge, G Nyoni, T Olajubo, R Opperman, M

Nemato, H Posthimus, C Keytel, JD Ntshudisane, BM Oosthuizen, DJ Van Dyk, K Silimela, M Truter, M Tshabalala, B Uaciquete, A Govender, V Gull, C Khalata, M

Jacobs, R Wolmarans, B Pretorius, M Schoeman, MV B. Buthelezi Millard, CP Domola, MJ Du Plooy, GW Du Preez, M Fourie A Gouws, R Phaswana, Y I Barnes

MSc – Students Microbiology: Front: Karen Surridge, Jaco le Roux, Francois du Plessis, Antoinette van Scalkwyk, Maree Pretorius and Chris Cooper. Back: Mapitsi Thantsha, Carin Joubert, Wanda Markotter, Sonya September,

Jacque Smit

POST GRADUATE STUDENTS


MSc – Students Plant Pahtology: Wilma van Broekhuizen, Fhumalani Mashau, Andries Fourie, Julia Domola, Veloshinie Govender, Kerien van Dyk and Mariëtte Truter

PhD-Students Jacobs, JA Coetser, SE Koster, E Kwerepe, BC Wentzel, JM Bartie, C Britz, H

Sabeta, CT Smith, JE Le Grange, C Slager-Bastos, A Adandonon, A Dube, R Jacobs, A

Nevondo, TS Obagwu, J Slippers, B Venter, SN Visser, M Thompson, AH Boshoff, M

Hugues, B Van den Berg, N Maseko, B Dube, R Van der Waals, JE Van der Linde, EJ Kritzinger, Q

PhD students: Raynard MacDonald, Joseph Hawumba, Boet Weyers, Brigetta Steyn, Tendani Nevondo, Marleen Kock and Julian Jaftha

Documents

Annual Report 2001 University of Pretoria · 2014-09-15 · 1 Jaarverslag / Annual Report 2001 University of Pretoria CONTENTS Message from the head of Department 2 Personnel 3 Extraordinary