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Annex 3.2.4.1 – Found documentation for A143 Investigation into the research of Milena Penkowa
Overview of paper A143. Zinc or copper deficiency-induced impaired inflammatory response to brain trauma may be caused by the concomitant metallothionein changes. Penkowa M, Giralt M, Thomsen PS, Carrasco J, Hidalgo J. J Neurotrauma. 2001 Apr;18(4):447-63. Similarities with A124.
Lab of the live animal exps and type of exps
Hidalgo. Mice fed with normal diets and subjected to cryolesion
No. & Types of animals used in the animal exps
3 or 6? mice of types 129/SvJ and MT I+II KO
MP’s exps and time of these exps
1999 or 2000. Stainings, counts, photos
Documentation and information requested by Panel, and further keywords and comments for
identifying the wanted documentation:
-Immunostained sections to confirm the findings in figure 9. Keywords: Mouse sections stained for/with: Lectin, GFAP, NSE, TUNEL, MT I+II, MDA, NITT, NF-
khi?Beta, iNOS, Cu,Zn-SOD paraffin embedded tissue.
The documentation and information found, including the “animal key”, if found: Keys:
D. Lesions. “MT-1 transgenic”. “MERCÉ” (i.e. Mercedes Giralt). Several D-Boxes with sections and some slide trays. EM. “E.M. experiment”: Lesions in mice, strains 129 and MT-I+II KO. (And, not relevant for A124 or A143): -“Experiment IL6 mice injected with KA”: mice, strains 129 and IL6 KO.) Page 1: Mail from X.Carrasco, 10.12.1998. Identification numbers of brains/animals. “Scannings-EM”. Page 2: handwritten list “For scanning-EM”. No EM-samples found. F. “Exp. Lesion in MT-3KO and 129Sv” “(02/2001)” “Animals no. 101-136”. (Mice). Page 2: F-animals, number 1-32. Several F-Boxes with sections were found.
-Several files found.
Written documentation found:
-Several files found on MP’s Hard-disc – are copied in the following, separated by “-- “ marks.
-(Followed by scanned copies of the found keys)
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File of 03.03.1999:
TUNEL RE-COUNTS. From unfixed ”for-in-situ-prepared” brains:
RE -COUNTINGS OF 3 LESIONED 129 at 2dpl
1. 29 2. 32 3. 36
RE-COUNTINGS OF 4 LESIONED female MT-I+II KO´S at 2dp l
1. 132 2. 129 3. 132 4. 125
RE -COUNTINGS OF 3 male LESIONED MT-III KO´S at 2dpl 1. 8 2. 15 3. 13
RE -COUNTINGS OF 3 UNLESIONED 129 1. 4 2. 5 3. 5
RE -COUNTINGS OF 3 female UNLESIONED MT-I+II KO´S
1. 4 2. 8 3. 11
RE -COUNTINGS OF 2 male UNLESIONED MT-III KO´S
3
1. 6 2. 5 -- File of 26.02.1999:
COUNTINGS OF 129 AND MT-I+II KO´S AND MT-III KO´S per 0.4 mm2 in ipsilateral cortex at 1, 3, 10 and 20 dpl.
Lectincells 129 mice 1 dpl: 1. 175 2. 177 3. 170 129 mice 3 dpl: 1. 140 2. 139 3. 144 129 mice 10 dpl: 1. 76 2. 77 3. 69 129 mice 20 dpl: 1. 11 2. 14 3. 9 MTKO´s 1 dpl: 1. 195 2. 190 3. 202 MTKO´s 3 dpl: 1. 160 (area with large cells), 180 (area with small cells) 2. 151, 178 3. 168, 185 MTKO´s 10 dpl:
4
1. 189 2. 177 3. 181 MTKO´s 20 dpl: 1. 173 2. 181 3. 199 GFAP+ cells 129 1 dpl: 1. 42 2. 54 3. 35 129 3 dpl: 1. 97 2. 91 3. 88 129 10 dpl: 1. 70 2. 77 3. 69 129 20 dpl: 1. 19 2. 12 3. 15 MTKO 1 dpl: 1. 36 2. 33 3. 29 MTKO 3 dpl: 1. 52 2. 47
5
3. 61 MTKO 10 dpl: 1. 98 2. 85 3. 93 MTKO 20 dpl: 1. 99 2. 92 3. 81 NSE+ cells: 129 1 dpl: 1. 23 2. 21 3. 18 129 3 dpl: 1. 25 2. 17 3. 19 129 10 dpl: 1. 16 2. 12 3. 17 129 20 dpl: 1. 15 2. 17 3. 15 MTKO 1 dpl: 1. 4 2. 2 3. 5 MTKO 3 dpl:
6
1. 3 2. 6 3. 1 MTKO 10 dpl: 1. 3 2. 4 3. 5 MTKO 20 dpl: 1. 5 2. 4 3. 5 MT-I+II: 129 mice 1 dpl: 1. 33 2. 28 3. 30 129 mice 3 dpl: 1. 77 2. 82 3. 80 129 mice 10 dpl: 1. 36 2. 40 3. 53 129 mice 20 dpl: 1. 4 2. 7 3. 3 MT-III KO 2 dpl: 1. 81 2. 79
7
-- File of 03.03.1999: SOD+ cells Unlesioned mice: 129 mice: 1. 11 2. 8 3. 14 MTKO mice: 1. 12 2. 7 3. 15 129 mice 1 dpl: 1. 35 2. 29 3. 28 129 mice 3 dpl: 1. 52 2. 59 3. 44 129 mice 10 dpl: 1. 36 2. 41 3. 32 129 mice 20 dpl: 1. 11 2. 5 3. 9 MTKO´s 1 dpl: 1. 35 2. 49
8
3. 42 MTKO´s 3 dpl: 1. 71 2. 60 3. 67 MTKO´s 10 dpl: 1. 62 2. 57 3. 69 MTKO´s 20 dpl: 1. 69 2. 56 3. 57 -- File of 03.03.1999:
”PREPARED FOR IN SITU-BRAINS”
NEW COUNTINGS OF THE 3 UNLESIONED MT-I+IIKO´S AND THE 3 UNLESIONED 129 MICE.
RE-COUNTINGS OF THE 2 UNLESIONED MT-III KO´S.
(thus all these data in this file have been counted the same day)
per 0.4 mm2 cortex
Lectin (ramified cells): 129 1. 61 2. 68 3. 74 MT-I+IIKO´S 1. 69 2. 67
9
3. 79 MT-IIIKO´S 1. 70 2. 68 ------------------------------------------------------------------------
GFAP: 129 1. 72 2. 71 3. 65 MT-I+IIKO´S 1. 73 2. 69 3. 66 MT-IIIKO´S 1. 79 2. 87 --------------------------------------------------------------------------------------------
NSE: 129 1. 29 2. 35 3. 32 MT-I+IIKO´S 1. 23 2. 34
10
3. 37 MT-IIIKO´s: 1 42 3. 45 ------------------------------------------------------------------------------
MT-I+II immunostainings: 129: 1. 7 2. 9 3. 12 MT-I+IIKO´s 1. 0 2. 0 3. 0 MT-IIIKO´s: 1. 10 2. 9 --------------------------------------------------------------------------------------------
MT-III immunostainings: 129: 1. 8 2. 9 3. 12 MT-I+IIKO´s 1. 10 2. 10 3. 9
11
MT-IIIKO´s: 1. 0 2. 0 ----------------------------------------------------------------------
GAP43+ cells 129: 1. 2 2. 3 3. 4 MT-I+IIKO´s: 1. 1 2. 2 3. 1 MT-IIIKO´s: 1. 4 2. 5 ---------------------------------------------------------------------
ssDNA: 129: 1. 3 2. 4 3. 2 MT-I+IIKO´s: 1. 5 2. 5 3. 7
12
MT-IIIKO´s: 1. 3 2. 2 -----------------------------------------------------------------------------------
TUNEL RE-COUNTS are mailed separately. -------------------------------------------------------------------------
ICE+ cells 129: 1. 2 2. 3 3. 3 MT-I+IIKO´s: 1. 4 2. 6 3. 9 MT-IIIKO´s: 1. 2 2. 4 --------------------------------------------------------------------------------
Caspase-3+ cells: 129: 1. 2 2. 3 3. 6
13
MT-I+IIKO´s: 1. 5 2. 7 3. 8 MT-IIIKO´s: 3. 2 4. 4 -- File of 04.05.2000 MERCE-EXPERIMENT WITH LESIONING, AND CONTROL OR MT OR ZN TREATMENT. As counted from 0.5 mm2 at the lesion borderline. LECTIN+ CELLS 129 mice treatment control round control ram MT+ round MT+ ram Zn+ ro Zn+ ram No lesion 0,2 74,69 0,1,1 72,70,71 2,2,4 76,68,75 3 dpl 162,157,159 46,44,42 84,78,88 25,28,29 169,
163,160 47,51,48
7 dpl 101,98,96 43,48,42 56,58,52 29,23,27 112,116, 123
50,46,45
MT-KO mice treatment control round control ram MT+ round MT+ ram Zn+ ro Zn+ r am No lesion 2,1 73,72 0,1 70,74 4,5 78,75 3 dpl 231,233,224 53,57,58 175,146,151 44,47,46 264,258,
261 66,53,59
7 dpl 197,182,187 55,52,48 101,101,116 36,35,42 211,217, 223
52,55,62
GFAP+ CELLS 129 mice
14
treatment control MT+ Zn+ No lesion 17,24 22,20,19 23,19,21 3 dpl 104,105,94 157,161,169 98,107,99 7 dpl 62,57,68 115,119,111 59,62,60
MT-KO mice treatment control MT+ Zn+ No lesion 15,25 23,24 19,23 3 dpl 62,56,59 93,104,102 63,65,60 7 dpl 41,34,36 82,81,76 44,31,35 MT-I+II+ CELLS 129 mice treatment control MT+ Zn+ controls 7,10 13,12,5 13,16,17 3 dpl 82,79,86 79,85,83 95,92,98 7 dpl 62,57,62 56,67,52 74,71,75
MT-KO mice treatment control MT+ Zn+ No lesion none none none 3 dpl none none none 7 dpl none none none Cu/Zn-SOD 129 mice treatment control MT+ Zn+ controls 14,12 11,14,13 17,18,14 3 dpl 58,60,61 37,42,39 68,73,67 7 dpl 45,40,42 30,34,32 51,52,49
MT-KO mice
15
treatment control MT+ Zn+ No lesion 12,15 10,12 15,18 3 dpl 69,73,75 59,60,58 86,80,77 7 dpl 59,57,58 51,48,44 65,68,70 MDA 129 mice treatment control MT+ Zn+ controls 2,1 1,2,1 1,3,4 3 dpl 53,59,55 33,29,34 57,62,59 7 dpl 37,38,42 17,20,21 39,37,43
MT-KO mice treatment control MT+ Zn+ No lesion 4,3 2,2 3,5 3 dpl 71,78,83 59,56,60 76,79,84 7 dpl 56,55,62 44,36,39 63,60,68 NITT 129 mice treatment control MT+ Zn+ controls 3,2 1,1,2 2,1,4 3 dpl 64,67,69 28,27,21 69,58,64 7 dpl 51,50,47 20,21,18 48,53,52
MT-KO mice treatment control MT+ Zn+ No lesion 2,2 3,1 2,3 3 dpl 95,109,103 71,62,70 98,106,107 7 dpl 80,84,78 56,65,53 81,93,87 TUNEL
16
129 mice treatment control MT+ Zn+ controls 2,0 0,0,2 1,2,2 3 dpl 38,38,36 14,8,15 39,43,37 7 dpl 36,35,36 10,12,13 37,30,39
MT-KO mice treatment control MT+ Zn+ No lesion 1,2 1,0 2,1 3 dpl 184,187,191 71,57,52 188,193,199 7 dpl 158,160,155 47,52,48 165,163,171 -- File of 06.10.2000 As counted from 0.5 mm2 at the lesion borderline. LECTIN+ CELLS 129 mice treatment round ram No lesion 0,1,2 64,71,67 3 dpl 157,151,149 46,44,42 7 dpl 114,109,94 43,48,42 14 dpl
MT-KO mice treatment round ram No lesion 2,1 73,72 3 dpl 231,233,224 53,57,58 7 dpl 197,182,187 55,52,48 14 dpl GFAP+ CELLS 129 mice treatment control MT+ Zn+ No lesion 17,24 22,20,19 23,19,21
17
3 dpl 104,105,94 157,161,169 98,107,99 7 dpl 62,57,68 115,119,111 59,62,60
MT-KO mice treatment control MT+ Zn+ No lesion 15,25 23,24 19,23 3 dpl 62,56,59 93,104,102 63,65,60 7 dpl 41,34,36 82,81,76 44,31,35 MT-I+II+ CELLS 129 mice treatment control MT+ Zn+ controls 7,10 13,12,5 13,16,17 3 dpl 82,79,86 79,85,83 95,92,98 7 dpl 62,57,62 56,67,52 74,71,75
MT-KO mice treatment control MT+ Zn+ No lesion none none none 3 dpl none none none 7 dpl none none none Cu/Zn-SOD 129 mice treatment control MT+ Zn+ controls 14,12 11,14,13 17,18,14 3 dpl 58,60,61 37,42,39 68,73,67 7 dpl 45,40,42 30,34,32 51,52,49
MT-KO mice treatment control MT+ Zn+
18
No lesion 12,15 10,12 15,18 3 dpl 69,73,75 59,60,58 86,80,77 7 dpl 59,57,58 51,48,44 65,68,70 MDA 129 mice treatment control MT+ Zn+ controls 2,1 1,2,1 1,3,4 3 dpl 53,59,55 33,29,34 57,62,59 7 dpl 37,38,42 17,20,21 39,37,43
MT-KO mice treatment control MT+ Zn+ No lesion 4,3 2,2 3,5 3 dpl 71,78,83 59,56,60 76,79,84 7 dpl 56,55,62 44,36,39 63,60,68 NITT 129 mice treatment control MT+ Zn+ controls 3,2 1,1,2 2,1,4 3 dpl 64,67,69 28,27,21 69,58,64 7 dpl 51,50,47 20,21,18 48,53,52
MT-KO mice treatment control MT+ Zn+ No lesion 2,2 3,1 2,3 3 dpl 95,109,103 71,62,70 98,106,107 7 dpl 80,84,78 56,65,53 81,93,87 TUNEL
19
129 mice treatment control MT+ Zn+ controls 2,0 0,0,2 1,2,2 3 dpl 38,38,36 14,8,15 39,43,37 7 dpl 36,35,36 10,12,13 37,30,39
MT-KO mice treatment control MT+ Zn+ No lesion 1,2 1,0 2,1 3 dpl 184,187,191 71,57,52 188,193,199 7 dpl 158,160,155 47,52,48 165,163,171 --
Copies of 9 files, (all 9 files are of Feb and March 1999 except one from
Oct 1999): --
TUNEL RE-COUNTS. From unfixed ”for-in-situ-prepared” brains:
RE -COUNTINGS OF 3 LESIONED 129 at 2dpl
4. 29 5. 32 6. 36
RE-COUNTINGS OF 4 LESIONED female MT-I+II KO´S at 2dp l
5. 132 6. 129 7. 132 8. 125
RE -COUNTINGS OF 3 male LESIONED MT-III KO´S at 2dpl
20
4. 8 5. 15 6. 13
RE -COUNTINGS OF 3 UNLESIONED 129 4. 4 5. 5 6. 5
RE -COUNTINGS OF 3 female UNLESIONED MT-I+II KO´S
4. 4 5. 8 6. 11
RE -COUNTINGS OF 2 male UNLESIONED MT-III KO´S
3. 6 4. 5 --
COUNTINGS OF 129 AND MT-I+II KO´S AND MT-III KO´S per 1 mm2 in ipsilateral cortex at 1, 3, 10 and 20 dpl.
MT-III stainings: 129 1 dpl: 1. 5 2. 5 3. 4 129 3 dpl: 1. 37 2. 42 3. 51 129 10 dpl:
21
1. 46 2. 43 3. 54 129 20 dpl: 1. 13 2. 11 3. 9 MTKO 1 dpl: 1. 145 2. 149 3. 139 MTKO 3 dpl: 1. 156 2. 157 3. 169 MTKO 10 dpl: 1. 157 2. 160 3. 163 MTKO 20 dpl: 1. 155 2. 157 3. 151 GAP43+ cells 129 mice 1 dpl: 4. 41 5. 39 6. 42 129 mice 3 dpl: 4. 72 5. 74 6. 79
22
129 mice 10 dpl: 4. 76 5. 81 6. 82 129 mice 20 dpl: 4. 81 5. 84 6. 79 MTKO´s 1 dpl: 4. 2 5. 3 6. 5 MTKO´s 3 dpl: 1. 8 2. 11 3. 15 MTKO´s 10 dpl: 4. 10 5. 17 6. 16 MTKO´s 20 dpl: 4. 13 5. 11 6. 19 ssDNA 129 1 dpl: 1. 43 2. 59 3. 52 129 3 dpl: 1. 56 2. 63
23
3. 57 129 10 dpl: 1. 50 2. 53 3. 57 129 20 dpl: 1. 5 2. 8 3. 7 MTKO 1 dpl: 1. 137 2. 144 3. 139 MTKO 3 dpl: 1. 181 2. 179 3. 186 MTKO 10 dpl: 1. 149 2. 161 3. 154 MTKO 20 dpl: 1. 150 2. 147 3. 159 ICE+ cells 129 1 dpl: 4. 62 5. 54 6. 57 129 3 dpl:
24
4. 66 5. 70 6. 73 129 10 dpl: 4. 31 5. 43 6. 36 129 20 dpl: 4. 6 5. 11 6. 4 MTKO 1 dpl: 4. 155 5. 153 6. 146 MTKO 3 dpl: 4. 169 5. 178 6. 180 MTKO 10 dpl: 4. 176 5. 171 6. 163 MTKO 20 dpl: 4. 172 5. 177 6. 159 Caspase-3+ cells: 129 1 dpl: 7. 51 8. 49 9. 50
25
129 3 dpl: 7. 52 8. 59 9. 61 129 10 dpl: 7. 32 8. 39 9. 28 129 20 dpl: 7. 2 8. 5 9. 1 MTKO 1 dpl: 7. 142 8. 139 9. 137 MTKO 3 dpl: 7. 169 8. 167 9. 158 MTKO 10 dpl: 7. 161 8. 159 9. 152 MTKO 20 dpl: 7. 159 8. 156 9. 143 --
COUNTINGS OF LESIONED MT-I+II KO´S AT 2 DPL AND LESIONED MT-III KO´S AT 2 DPL
per 0.4 mm2
26
At 2 dpl:
Lectincells MT-IIIKO (3 females): 7. 132 8. 127 9. 140 MT-I+IIKO´s (4 females) 1. 140 (area with large cells), 171 (area with small cells) 2. 143, 168 3. 152, 175 4. 155 164
GFAP+ cells MT-IIIKO´s: 10. 98 11. 88 12. 91 MT-I+IIKO: 10. 42 11. 44 12. 51 13. 45 NSE+ cells:
MT-IIIKO´s: 1. 23 2. 21 3. 26
MT-I+IIKO
27
4. 1 5. 4 6. 1 7. 3 MT-I+II: MT-IIIKO´s: 4. 69 5. 88 6. 81 MT-I+IIKO´s: 1. 0 2. 0 3. 0 4. 0 --
COUNTINGS OF 129 fixed per 1 mm2 in ipsilateral cortex at 2 dpl.
Lectin: 1. 132 2. 128 3. 116 GFAP: 1. 85 2. 90 3. 89 NSE: 1. 20 2. 18 3. 19 MT-III stainings: 4. 25
28
5. 28 6. 14 GAP43+ cells 10. 58 11. 44 12. 47 ssDNA 4. 47 5. 60 6. 53 ICE+ cells 10. 63 11. 59 12. 67 Caspase-3+ cells: 1. 61 2. 60 3. 58 --
COUNTINGS OF UNLESIONED 129 AND MT-I+II KO´S CORTEX , AND LESIONED MT-III KO´S at 2 dpl.
per 1 mm2 cortex
MT-III stainings: Unles. 129: 1. 13 2. 9 3. 16 Unles. MT-I+IIKO: 1. 17 2. 14 3. 10
29
MT-IIIKO at 3 dpl: 3. 0 4. 0 5. 0 GAP43+ cells Unles. 129 mice: 1. 1 2. 0 3. 0 Unles. MT-I+IIKO: 1. 0 2. 0 3. 0 MT-IIIKO mice at 3 dpl: 1. 108 2. 89 3. 99 ssDNA Unles. 129: 3. 3 4. 7 5. 6 Unles. MT-I+IIKO: 1. 6 2. 10 3. 6 MT-IIIKO at 3 dpl: 1. 53 2. 51 3. 56
30
ICE+ cells Unles. 129: 1. 2 2. 5 3. 7 Unles. MT-I+IIKO: 1. 4 2. 7 3. 3 MT-IIIKO at 3 dpl: 1. 71 2. 63 3. 66 Caspase-3+ cells: Unles. 129: 1. 2 2. 5 3. 1 Unles. MT-I+IIKO: 1. 3 2. 5 3. 4 MT-IIIKO at 3 dpl: 1. 57 2. 62 3. 74 --
COUNTINGS OF MT-I+II KO´S AT 2 DPL per 1 mm2
31
At 2 dpl:
Lectincells 1. 140 (area with large cells), 171 (area with small cells) 2. 143, 168 3. 152, 175 4. 155 164
GFAP+ cells 14. 59 15. 55 16. 61 17. 67 NSE+ cells: 8. 9 9. 8 10. 7 11. 12 MT-I+II: 5. 0 6. 0 7. 0 8. 0 MT-III:
1. 62 2. 51 3. 54 4. 79
GAP-43:
1. 31 2. 29
32
3. 32 4. 20
ssDNA:
1. 143 2. 139 3. 146 4. 151
ICE:
1. 150 2. 142 3. 146 4. 149
Caspase-3:
1. 148 2. 153 3. 155 4. 138
COUNTINGS OF 129 at 2 dpl per 1 mm2 in ipsilateral cortex.
Lectin: 1. 132 2. 128 3. 116 GFAP: 1. 85 2. 90 3. 89 NSE: 1. 20 2. 18 3. 19
33
MT-I+II:
1. 129 2. 136 3. 131 4. 144
MT-III stainings: 1. 25 2. 28 3. 14 GAP43+ cells 1. 58 2. 44 3. 47 ssDNA 1. 47 2. 60 3. 53 ICE+ cells 1. 63 2. 59 3. 67 Caspase-3+ cells: 1. 61 2. 60 3. 58
TUNEL RE-COUNTS. From unfixed ”for-in-situ-prepared” brains:
34
RE -COUNTINGS OF 3 LESIONED 129 at 2dpl
1. 29 2. 32 3. 36
RE-COUNTINGS OF 4 LESIONED female MT-I+II KO´S at 2dp l
1. 132 2. 129 3. 132 4. 125
--
COUNTINGS OF UNLESIONED 129 AND MT-I+II KO´S CORTEX , AND LESIONED MT-III KO´S at 2 dpl.
per mm2 cortex
MT-III stainings: Unles. 129: 4. 13 5. 9 6. 16 Unles. MT-I+IIKO: 4. 17 5. 14 6. 10 MT-IIIKO at 3 dpl: 4. 0 5. 0 6. 0 GAP43+ cells Unles. 129 mice:
35
4. 1 5. 0 6. 0 Unles. MT-I+IIKO: 7. 0 8. 0 9. 0 MT-IIIKO mice at 3 dpl: 7. 108 8. 89 9. 99 ssDNA Unles. 129: 4. 3 5. 7 6. 6 Unles. MT-I+IIKO: 4. 6 5. 10 6. 6 MT-IIIKO at 3 dpl: 4. 53 5. 51 6. 56 ICE+ cells Unles. 129: 4. 2 5. 5 6. 7 Unles. MT-I+IIKO:
36
13. 4 14. 7 15. 3 MT-IIIKO at 3 dpl: 10. 71 11. 63 12. 66 Caspase-3+ cells: Unles. 129: 7. 2 8. 5 9. 1 Unles. MT-I+IIKO: 16. 3 17. 5 18. 4 MT-IIIKO at 3 dpl: 13. 57 14. 62 15. 74 --
”PREPARED FOR IN SITU-BRAINS”
NEW COUNTINGS OF THE 3 UNLESIONED MT-I+IIKO´S AND THE 3 UNLESIONED 129 MICE.
RE-COUNTINGS OF THE 2 UNLESIONED MT-III KO´S.
(thus all these data in this file have been counted the same day)
per 0.4 mm2 cortex
37
Lectin (ramified cells): 129 4. 61 5. 68 6. 74 MT-I+IIKO´S 4. 69 5. 67 6. 79 MT-IIIKO´S 3. 70 4. 68 ------------------------------------------------------------------------
GFAP: 129 4. 72 5. 71 6. 65 MT-I+IIKO´S 4. 73 5. 69 6. 66 MT-IIIKO´S 4. 79 5. 87 --------------------------------------------------------------------------------------------
38
NSE: 129 4. 29 5. 35 6. 32 MT-I+IIKO´S 4. 23 5. 34 6. 37 MT-IIIKO´s: 2 42 6. 45 ------------------------------------------------------------------------------
MT-I+II immunostainings: 129: 4. 7 5. 9 6. 12 MT-I+IIKO´s 4. 0 5. 0 6. 0 MT-IIIKO´s: 1. 10 2. 9 --------------------------------------------------------------------------------------------
39
MT-III immunostainings: 129: 4. 8 5. 9 6. 12 MT-I+IIKO´s 4. 10 5. 10 6. 9 MT-IIIKO´s: 6. 0 7. 0 ----------------------------------------------------------------------
GAP43+ cells 129: 4. 2 5. 3 6. 4 MT-I+IIKO´s: 4. 1 5. 2 6. 1
40
MT-IIIKO´s: 3. 4 4. 5 ---------------------------------------------------------------------
ssDNA: 129: 4. 3 5. 4 6. 2 MT-I+IIKO´s: 4. 5 5. 5 6. 7 MT-IIIKO´s: 6. 3 7. 2 -----------------------------------------------------------------------------------
TUNEL RE-COUNTS are mailed separately. -------------------------------------------------------------------------
ICE+ cells 129:
41
4. 2 5. 3 6. 3 MT-I+IIKO´s: 4. 4 5. 6 6. 9 MT-IIIKO´s: 5. 2 6. 4 --------------------------------------------------------------------------------
Caspase-3+ cells: 129: 4. 2 5. 3 6. 6 MT-I+IIKO´s: 4. 5 5. 7 6. 8 MT-IIIKO´s: 7. 2 8. 4 --
COUNTINGS OF THE 2 UNLESIONED MT-III KO´S. per 0.4 mm2 cortex
Lectin (ramified cells): 1. 72 2. 78
42
GFAP: 1. 82 2. 91 NSE: 1 49 1. 51 MT-I+II: 1. 8 2. 9 MT-III stainings: 1. 0 2. 0 GAP43+ cells 1. 3 2. 2 ssDNA 1. 3 2. 2 Tunel: 1. 4 2. 5 ICE+ cells 1. 1 2. 4 Caspase-3+ cells: 1. 4 2. 3 --
(3 files, of Sep and Oct 2000):
LESIONED 129 + MT TRANSGENIC MICE
As counted from 0.5 mm2 at the lesion borderline or from spleen white and red pulp. Juan, I counted from 4 mice in each group, however, in 129 mice at 1 dpl and in controls, I´ve counted from 3 mice.
43
CNS CD3+ CELLS animal 129 MT-TR controls 0,0,1, 0,0,0 1 dpl 29,34,32 12,15,9,11 3 48,51,50,53 16,12,11,17 6 33,30,35,31 10,13,10,9 10 24,25,22,19 8,10,7,5 CD4+ CELLS animal 129 MT-TR controls 1,1,0 1,0,0 1 dpl 27,33,30 10,14,9,10 3 47,49,50,51 15,17,11,10 6 30,29,34,30 10,12,10,8 10 22,23,21,19 7,9,7,5 CD8+ CELLS animal 129 MT-TR controls 0,0,0 0,0,0 1 dpl 3,2,2 2,1,1,2 3 4,4,2,2 3,2,2,2 6 3,2,1,1 2,3,2,0 10 3,2,1,0 2,0,1,1
SPLEEN CD3+ CELLS animal 129 MT-TR controls 199,202,205 203,200,200 1 dpl 361,378,375 365,382,381,384 3 401,404,400,403 406,403,401,400 6 311,308,312,307 306,307,314,310 10 254,257,251,248 246,255,258,252 --
LESIONED 129 + MT TRANSGENIC MICE
44
As counted from 0.5 mm2 at the lesion borderline. Juan, I counted from 4 mice in each group, however, in 129 mice at 1 dpl, there´re only 3 mice. LECTIN+ CELLS animal 129 round 129 ram MT-TR round MT-TR ram controls 0,1,1 69,72,68 0,0,0 73,66,70 1 dpl 110,98,107 43,41,48,36 35,38,34,36 26,22,20,29 3 151,155,157,149 40,38,44,41 77,80,82,79 15,19,12,13 6 92,96,91,84 41,45,43,49 6,9,11,4 47,38,37,43 10 69,65,60,53 52,55,48,51 8,8,3,4 45,48,51,47
GFAP+ CELLS animal 129 MT-TR controls 19,17,22,23 21,25,22,18 1 dpl 65,61,58 93,96,87,90 3 101,104,109,94 174,169,158,165 6 82,85,77,79 133,129,134,136 10 63,61,56,64 96,88,92,97 MT-I+II+ CELLS animal 129 MT-TR controls 7,8,8,11 38,42,35,39 1 dpl 32,36,29 61,66,73,64 3 99,85,92,98 138,156,153,160 6 87,91,75,69 108,105,114,120 10 30,47,29,38 99,95,92,111 Cu/Zn-SOD animal 129 MT-TR controls 9,12,18,11 11,12,10,17 1 dpl 30,35,39 41,26,23,34 3 68,56,63,69 58,46,54,47 6 72,61,67,55 52,44,41,55 10 69,65,74,57 59,54,42,38 MDA
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animal 129 MT-TR controls 2,3,3,7 0,2,1,1 1 dpl 25,29,33 12,15,15,14 3 52,55,59,64 22,29,31,30 6 39,42,45,40 17,25,18,21 10 17,27,29,16 8,11,7,5 NITT animal 129 MT-TR controls 1,1,4,2 0,1,1,0 1 dpl 34,28,35 10,13,12,15 3 61,64,59,68 21,23,17,20 6 53,57,59,43 16,12,15,9 10 27,32,36,37 9,5,10,11 TUNEL animal 129 MT-TR controls 0,1,3,1 0,0,1,0 1 dpl 27,22,23,19 11,8,10,7 3 38,46,35,37 12,10,14,9 6 32,39,41,36 19,7,5,8 10 24,20,18, 32 6,11,10,4 CYTOKINES IL-1 ββββ animal 129 MT-TR controls 0,0,1,0 0,1,0,1 1 dpl 24,29,32 10,14,13,9 3 81,75,77,89 26,40,30,37 6 62,66,57,59 15,31,16,13 10 33,45,42,38 7,9,4,8 IL-6 animal 129 MT-TR controls 1,1,0,0 0,0,1,2 1 dpl 35,38,39 11,12,9,11
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3 88,85,89,92 24,41,34,30 6 86,87,91,89 22,21,32,31 10 81,86,90,76 19,16,13,15 TNF-αααα animal 129 MT-TR controls 1,1,1,0 1,1,0,0 1 dpl 28,35,33 12,15,14,11 3 84,78,79,85 25,37,32,39 6 64,67,59,58 14,33,19,11 10 35,46,45,39 9,12,5,6 GF´s bFGF animal 129 MT-TR controls 10,9,11,8 10,10,9,9 1 dpl 102,101,100 139,135,130,131 3 144,138,147,150 235,227,212,229 6 101,102,99,102 154,161,159,162 10 92,88,85,89 136,133,129,138 TGF-ββββ1 animal 129 MT-TR controls 12,9,14,9 11,12,12,7 1 dpl 105,104,101 147,143,151,150 3 148,145,156,151 247,248,246,252 6 110,109,107,106 159,164,166,161 10 93,89,86,93 148,151,144,142 NT-3 animal 129 MT-TR controls 7,9,10,6 5,9,9,8 1 dpl 100,99,95 122,125,120,131 3 133,136,137,142 225,217,219,215 6 96,92,97,103 146,151,149,153 10 87,86,88,90 125,123,120,124
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MT TRANSGENICS AND 129 MICE SPLEEN LECTIN+ CELLS (counted from 0.5 mm2 of both white and red pulp) animal 129 MT-TR controls 303,307,299,291 296,302,297,199 1 dpl 368,389,371,377 118,135,105,139 3 448,449,454,471 213,202,211,190 6 379,387,380,381 109,112,107,101 10 313,320,291,309 73,75,88,85 -- --End of files found Scanned copies of found keys on the following pages:
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