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Figure 1.2 The hierarchy of biological organization At each level of increasing complexity, new properties emerge. At what level do we have “life”?
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Life!
And how we try to figure it out
Figure 1.2 The hierarchy of biological organization
Biology
• Define life (good luck!).• How do we know a living thing
when we see one?– list characteristics common to
ALL living things.
Characteristics of all living things:
1. Made Up of Cells - Organization2. Reproduction3. Based on DNA4. Growth and Development5. Need for Energy6. Response to Environmental Stimuli7. Homeostasis8. Evolution
What makes Biology a science?
• How do you know science when you see it?– list some characteristics of
science and describe how scientists work.
Science is…• Objective• Data driven• Experimental• Attempt to find the best possible
naturally occurring explanation for a phenomenon.
Observations are objective• Observe the familiar actions and
describe them. No talking!• Compare notes with others – how
objective were your observations?• What happens when new observations
don’t fit with the old?• How did observations lead to
hypotheses on life?
Where does life come from?• At least by Aristotle’s time, people were
concerned with this.• Prevailing theory: Spontaneous
generation or Abiogenesis– Life comes from non-living things.– Based on everyday observations– Aristotle described an “active principle”
that was responsible.
Abiogenesis examples• Salamanders and frogs come from river
mud.• Mice come from wheat that has been
exposed to sweat.• Humans???
– pregnancy and birth were understood, sexual intercourse was important, but sperm and eggs didn’t play a role!
Really small life?• Microscopes allowed observation of
micro-organisms, but these were thought to come from plants or mud mixed with water (van Leeuwenhoek, mid 1600’s).
• Bacteria in meat broth were formed from the meat (Needham, 1745).
An alternative view: Biogenesis
• By the mid 17th century some individuals questioned abiogenesis.
• Their observations suggested all living things were produced by other living things– Biogenesis
• A test of biogenesis/abiogenesis was needed!
Francisco Redi• 1668: An Italian physician decided to
test the “common knowledge” that maggots came from rotting meat.– Without refrigeration or concern about
covering food, maggots tended to appear on meat within a few days. Therefore, the meat produced the maggots.
• OBSERVATION: Flies land on meat that is left uncovered. Later, maggots appear on the meat.
• HYPOTHESIS: Flies produce maggots.
Redi’s Alternative:
Redi’s Experimental Design• If flies are kept away from meat by a
covering, no maggots should appear.• Compare to meat stored in the usual
way (uncovered) which should develop maggots.
• Keep every other factor the same!
The variables:• Independent variable:
–covering over jar or not• Controls fly access to meat
• Controlled Variables:–jars, type of meat, location,
temperature, time• Dependent variable:
–maggots?
Uncovered jars Covered jars
Several days pass
?
Procedure
?
Results
Uncovered jars Covered jars
Several days pass
Maggots appear No maggots appear
CONCLUSION:
• Maggots form only when flies come in contact with meat. – Spontaneous generation of
maggots did not occur.
Spallanzani’s Experiment: 1765
Gravy is boiled. Flask isopen.
Gravy is teeming with microorganisms.
Figure 1-10 Spallanzani’s Experiment
Gravy is boiled. Flask issealed.
Gravy is free of microorganisms.
Pasteur’s Experiment: 1860
Broth is boiled.
Pasteur
Broth is boiled. Broth is free ofmicroorganismsfor a year.
Pasteur
Broth is boiled. Broth is free ofmicroorganismsfor a year.
Curved neckis removed.
Pasteur
Broth is boiled. Broth is free ofmicroorganismsfor a year.
Curved neckis removed.
Broth is teeming with microorganisms.
Biogenesis wins!• Pasteur’s experiments finally won
general support for the biological origins of living things.
• Pasteur also introduced methods of food preservation (Pasteurization!) that dramatically increased the shelf life of foods.
But wait! There’s more!
• Where did the FIRST living thing come from???
• Where did the universe come from???
• Let’s start with nonliving things and how they affect living things.
• Abiotic vs Biotic factors!