Analysis of Tropicana Orange Juice for Calcium & Vitamin C

Kate Penrod & Ashley Kimble

Objective

• Calculate Calcium content• Calculate Vitamin C content• Compare to Tropicana’s claims

Importance of Experiment

• Calcium– Most abundant mineral– Regulates cellular processes

• Vitamin C– Reducing agent– Antioxidant

Failed Procedures

• Procedure 1– No filtration– No dilution

• Procedure 2– Filtration through glass

wool– Dilution with water

• Procedure 3– Filtration through glass

wool– Dilution with water– Centrifuge

Procedure

• Procedure 4– Filtration through glass

wool– Dilution with HCl– Centrifuge

EDTA Titration of Tropicana Calcium Plus DTrial Grams of Calcium % Error

1 0.009925500 g 40.94210058%2 0.009908944 g 40.70700116%3 0.009805467 g 39.23762981%4 0.009797189 g 39.12008010%Manufacturer’s Claim 0.007042254 g 40.00170291%

EDTA Titration of Tropicana Calcium Plus D (Spiked)Trial Grams of Calcium % Error

1 0.008929833 g 18.00563207%2 0.008958853 g 18.38912298%3 0.008909104 g 17.73170999%Manufacturer’s Claim 0.007567294 g 18.04215501%

Iodimetric Titration of Tropicana Calcium Plus DTrial Grams of Ascorbic Acid % Error

1 0.002199092 g 65.30321983%2 0.002334700 g 63.16361710%3 0.003080548 g 51.39580206%Manufacturer’s Claim 0.006338028 g 59.95421300%

Iodimetric Titration of Tropicana Calcium Plus D (Spiked)

Trial Total Expected Grams of AA

Grams of Ascorbic Acid

% Error

1 0.006869014 g 0.002798167 g 59.26391531%2 0.007069014 g 0.003142279 g 55.54855584%3 0.006669014 g 0.003142279 g 52.88240797%

Average 55.89829304%

Iodimetric Titration of Tropicana RegularTrial Grams of Ascorbic Acid % Error

1 0.004587547 g 27.61870362%2 0.004381080 g 30.87629205%3 0.003899324 g 38.47733171%Manufacturer’s Claim 0.006338028 g 32.32410912%

Iodimetric Titration of Tropicana Regular(Spiked)

Trial Total Expected Grams of AA

Grams of Ascorbic Acid

% Error

1 0.006869014 g 0.004243436 g 38.22351435%2 0.007269014 g 0.004656369 g 35.94221733%3 0.006869014 g 0.004449902 g 35.21774279%

Average 36.46115816%

Discussion & Conclusion

• Must simulate digestion• Particles interfere with endpoint• 6% DV Magnesium• Vitamin C particles potentially large• Vitamin C spike?