Analette Manual Service Rev. D 2-6-08

2900 Series

Service Manual

1997-2006 Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is restricted for use by factory authorized personnel and is to be used for the sole purpose of providing routine instrument maintenance and repair service. No part of this manual may be copied or redistributed without the express consent of Awareness Technology, Inc. There is not transfer of technology, copyright, trade name, patent, trade secret, or other proprietary right given or implied. Rev. D 08/2006

TABLE OF CONTENTS

1. INTRODUCTION.......................................................................................................1

1.1 What ChemWell does.........................................................................................................................................1

1.2 Warnings and Precautions.................................................................................................................................2 1.2.1 Intended Use .................................................................................................................................................2 1.2.2 General Safety Summary ..............................................................................................................................4 1.2.3 To Avoid Fire or Personal Injury..................................................................................................................4 1.2.4 Safety Terms and Symbols ...........................................................................................................................5

1.3 Layout..................................................................................................................................................................6

1.4 Technical Specifications ...................................................................................................................................21

2. PRINCIPLES OF OPERATION...............................................................................23

2.1 System Control / Electronics Rack..................................................................................................................23

2.2 What Main Controls.........................................................................................................................................24

2.3 What Coprocessor Controls ............................................................................................................................25

2.4 What Daughter Controls..................................................................................................................................26

2.5 All Junction Boards..........................................................................................................................................28

2.6 Main and Coprocessor, How They Communicate .........................................................................................33 2.6.1 Firmware versus Software ..........................................................................................................................33

2.7 Power Supply ....................................................................................................................................................34

2.8 Watchdog Circuit .............................................................................................................................................35

2.9 Motion Control .................................................................................................................................................36

2.10 Plumbing .........................................................................................................................................................37 2.10.1 Pressure and Vacuum................................................................................................................................37 2.10.2 Pumps and Valves.....................................................................................................................................38 2.10.3 Wash System ............................................................................................................................................38 2.10.4 Draining ...................................................................................................................................................39

2.11 Bottle Fill Level Sensing.................................................................................................................................39

2.12 Syringe Pumps ................................................................................................................................................39

2.13 Photometer ......................................................................................................................................................39 2.14 Computer Connections ................................................................................................................................41

2.15 Computer Software Interface........................................................................................................................42 2.15.1 Explanation of terms.................................................................................................................................42 2.15.2 Navigating the software ............................................................................................................................43 2.15.3 ChemWell status window .........................................................................................................................43

2.16 Instrument Functions.....................................................................................................................................43

3. TROUBLESHOOTING............................................................................................44

3.1 Service Tools .....................................................................................................................................................44 3.1.1 Test Mode ...................................................................................................................................................44 3.1.2 Status Indicators..........................................................................................................................................44 3.1.3 Reports........................................................................................................................................................46 3.1.4 Updating the default reports .......................................................................................................................46 3.1.5 System Info Report .....................................................................................................................................46 3.1.6 Using ChemWell Pack................................................................................................................................47 3.1.7 Instrument Report .......................................................................................................................................48 3.1.8 Software Service Commands......................................................................................................................50 3.1.9 Available Tests Commands ........................................................................................................................50 3.1.10 General Commands ..................................................................................................................................51 3.1.11 Plate X/Y Control .....................................................................................................................................51 3.1.12 Mixing ......................................................................................................................................................51 3.1.13 Wash Head Control...................................................................................................................................51 3.1.14 Probe X/Z Control ....................................................................................................................................52 3.1.15 Temperature Control.................................................................................................................................52 3.1.16 Rack Control.............................................................................................................................................52 3.1.17 Pressure/Vacuum and Valve Control........................................................................................................52 3.1.18 Diluter Control..........................................................................................................................................53 3.1.19 Photometer................................................................................................................................................53 3.1.20 Probe Macros ............................................................................................................................................54 3.1.21 Washer Functions .....................................................................................................................................54 3.1.22 Service Functions......................................................................................................................................55

3.2 Error Messages .................................................................................................................................................55

3.3 Valves.................................................................................................................................................................58

3.4 Vacuum and Pressure Systems........................................................................................................................58

3.5 Motor Control...................................................................................................................................................58

3.6 Incomplete Aspiration......................................................................................................................................60

3.7 Incomplete or Inaccurate Dispense.................................................................................................................60

3.8 Photometer ........................................................................................................................................................60 3.8.1 Photometer Test Points ...............................................................................................................................62

3.9 Plate Temperature Control..............................................................................................................................62

3.10 Coil Temperature Control .............................................................................................................................63

3.11 Probe Temperature Control ..........................................................................................................................63

3.12 Carrier Temperature Control .......................................................................................................................63

3.13 External Temperature Probe ........................................................................................................................63

3.14 Serial Port .......................................................................................................................................................64

3.15 Printers ............................................................................................................................................................65 3.15.1 Black & White ..........................................................................................................................................65 3.15.2 Color .........................................................................................................................................................65

4. SERVICE PROCEDURES ......................................................................................66

4.1 Replacing Probe Tip or Probe Assembly........................................................................................................68

4.2 Bottles ................................................................................................................................................................69 4.2.1 Clogged Tubing ..........................................................................................................................................69 4.2.2 Bottle Sensors .............................................................................................................................................69 4.2.3 Hydrophobic Filter Replacement................................................................................................................69 4.2.4 Pressure Leak..............................................................................................................................................70

4.3 Chassis ...............................................................................................................................................................70 4.3.1 Dome Lamp Replacement ..........................................................................................................................70

4.4 Electronics Rack ...............................................................................................................................................71 4.4.1 AC Wiring Layout ......................................................................................................................................71 4.4.2 Board Replacement.....................................................................................................................................71 4.4.3 EPROM Replacement.................................................................................................................................71 4.4.4 Fuse Replacement .......................................................................................................................................72

4.5 Photometer ........................................................................................................................................................73 4.5.1 Photometer Output Check..........................................................................................................................74 4.5.2 Lamp Replacement .....................................................................................................................................74 4.5.3 Adjust Lamp Bracket Level Screws ...........................................................................................................76 4.5.4 Exhaust Fan ................................................................................................................................................76

4.6 Photometer - Assembly Placement..................................................................................................................76 4.6.1 Adjust Photometer ......................................................................................................................................76 4.6.2 Photometer Mount and Adjustment ............................................................................................................76

4.7 Filter Wheel.......................................................................................................................................................77 4.7.1 Filter Label .................................................................................................................................................77 4.7.2 Photometer Filter Replacement...................................................................................................................78

4.8 Plate Carrier .....................................................................................................................................................81

4.9 Hydraulic System..............................................................................................................................................82 4.9.1 Syringe Pump..............................................................................................................................................82 4.9.2 Syringe Replacement ..................................................................................................................................83 4.9.3 Fittings: Tubing, fittings, etc.......................................................................................................................84 4.9.4 Repair Tubing .............................................................................................................................................84

4.10 Valve and Pressure Pump..............................................................................................................................85 4.10.1 Valve Tubing Replacement ......................................................................................................................85 4.10.2 Wash Dispense and Rinse Dispense Valve Replacement .........................................................................87 4.10.3 Bleed or Control Valve Replacement .......................................................................................................87

4.11 Washer.............................................................................................................................................................88

5. CALIBRATION........................................................................................................90

5.1 Setup ..................................................................................................................................................................90

5.2 Photometer ........................................................................................................................................................90

5.3 External Sensor.................................................................................................................................................90

5.4 Plate Carrier .....................................................................................................................................................90

5.5 Coil/Block ..........................................................................................................................................................90

5.6 Vacuum..............................................................................................................................................................91

5.7 Pressure .............................................................................................................................................................92

5.8 Trimpots on Daughter PCA.............................................................................................................................92

6. ALIGNMENT ...........................................................................................................93

6.1 Photometer/Plate Carrier ................................................................................................................................93

6.2 Rack 1/Rack2 ....................................................................................................................................................94

6.3 Dispense.............................................................................................................................................................94

6.4 Wash Cup..........................................................................................................................................................95

6.5 Washer...............................................................................................................................................................95

7. CONTACT INFORMATION.....................................................................................97

APPENDIX A: PARTS ................................................................................................98

ChemWell Replacement Parts and Accessories...................................................................................................98 Selected Part Illustrations ..................................................................................................................................102

Parts by Sub-Assembly ........................................................................................................................................106

APPENDIX B: SYSTEM DIAGRAM .........................................................................107

APPENDIX C: BLOCK DIAGRAM ...........................................................................108

APPENDIX D: PCB LAYOUTS REVISION N ........................................................109

APPENDIX E: INTERNAL PLUMBING ....................................................................111

APPENDIX F: SCHEMATICS 100 REVISION N ...................................................113

APPENDIX G: SCHEMATICS 200 REVISION J ...................................................123

APPENDIX H: SCHEMATICS 300 REVISION H ...................................................130

APPENDIX I: SCHEMATICS ON CD (ENCLOSED) ................................................141

2900 Service Manual Rev. D Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument maintenance and repair services.

1

1. INTRODUCTION

1.1 What ChemWell does ChemWell is a computer controlled instrument system, capable of automating any or all stages of assay processing that involve these functions:

fluid handling .........aspirating and dispensing from 2 uL to 2.5 mL incubating..............heating to 25C or 37C: probe/coil to 37C or ambient, and reaction plate to 25C, 37C, or ambient. Mixing....................reaction plate only strip washing .........8-wells simultaneously timing.....................from 1 sec to 24 hours optical reading.......UV/Visible range calculating .............using numerous preprogrammed equations data storage ..........unlimited capacity reporting ................with many options and customization to choose from.

The system allows you to define and program an unlimited number of customized protocols by selecting displayed menu options from a Microsoft Windows1 software program. This open system can be programmed to perform any of the colorimetric biochemistry assays or EIA assays that can be handled using the volumes, temperatures, and wavelengths provided. It has many possible applications in clinical and veterinarian testing, environmental testing, food and water analysis, life science research, and may also be used in production processes involving micro volume dispensing, diluting, incubating, reading, washing, and so on.

ChemWell is a unique combination of an automatic chemistry system and an automated EIA system, two instruments in one.

Reactions occur in standard plastic microwells instead of sample cups or a carousel. Microwell strips and plates are commercially available from many sources. Place your reagent bottles and sample tubes into the removable instrument racks. Then program the instrument to pick up from one place, dispense to another, wash the probe, wash the plate, read the wells, incubate, mix, or whatever you want it to do. When doing chemistry reactions, groups of four wells are timed simultaneously. Rows of 8 are timed together to coordinate EIA processes.

ChemWell is not dedicated for use with any particular chemical reaction, method, or manufacturer. This gives you many advantages including great flexibility in how you use it. Your lab decides how to set up your racks and plates, which reagents you will use, how many controls you will run, how many different applications you want on it, and so on. This also means that the instrument must first be programmed before each new application.

To assure the quality of clinical information, each new setup must be validated before reporting specimen results. In some cases the programming, optimization, and validation may have already been done. Before applying any new clinical reagent system, check first with the diagnostic reagent manufacturer for specific instructions, validation information, and application tips. You are also advised to run specimens having known concentrations to verify your instrument setup parameters. After that, programs can easily be recalled for review, use, change, or deletion by you. You decide everything including how manual and how automatic you want your system to be.

Awareness Technology, Inc. developed ChemWell instruments and software as tools for the modern global laboratory. The power and freedom to apply ChemWell, is completely yours.


2

1.2 Warnings and Precautions

WARNING For in-vitro diagnostics use.

1.2.1 Intended Use The ChemWell is designed for use in processing general chemistry and enzyme-linked immunosorbent assays (ELISA or EIA), including clinical diagnostic assays, requiring multi-step washing, rinsing, and soaking. This general purpose instrument is intended to be used by laboratory professionals who are capable of selecting the appropriate features and options for each specific clinical application.

Some diagnostic assays utilize materials which are potentially biohazardous.

Always wear protective apparel and eye protection while using this instrument.

Always operate the instrument with the aerosol shield lowered.

Do not use the instrument in a manner not specified by the manual, or the protection provided by the instrument may be impaired.

Probe tips are sharp and may cause bodily injury. Do not place hands or fingers under the probe or wash head probes while instrument is in operation. Always set the power switch to OFF (0) before working on the probe or wash head. Never touch the probe or wash head while the instrument is operating.

WARNING

The probe performs a self-clean periodically while the probe is idle. Keep hands away from the probe at all times when the instrument is ON (1).

If the waste bottle is overturned during operation, immediately set the power switch to OFF (0). If the hydrophobic filter becomes wet due to an overturned waste bottle, it will be blocked. Continued use of a blocked filter will impair washer effectiveness and/or result in damage to the instrument.

The wash and rinse bottles are pressurized during normal operation. Do not remove bottle caps or tubing connections while the bottles are pressurized. Turn off the instrument before changing bottles, adding more solution, or tubing connections.

Solvents such as acetone or thinner will damage the instrument. Do not use solvents to clean the unit. Avoid abrasive cleaners; the aerosol shield is liquid-resistant, but is easily scratched.

The exterior of the instrument may be cleaned with a soft cloth using plain water. If needed, a mild all-purpose or non-abrasive cleaner may be used. A 10% solution of chlorine bleach (chlorine bleach= 5.25% Sodium Hypochlorite) or 70% isopropyl alcohol may be safely used as a disinfectant. Take special care not to spill liquid inside the instrument.

!

!


3

Particulate matter in wash solutions can clog washer probe head easily. See the section on cleaning the probe head for special instructions on removing particulate matter from clogged washer heads.

Please take time to read this manual carefully before using the instrument. For best results, familiarize yourself with the instrument and its capabilities before attempting any clinical diagnostic tests. Refer any questions to your instrument dealer.

Retain the original packing material for future use in the event that the instrument is placed in storage, shipped to another location, or returned for service. Two people or more should lift the instrument by placing hands under the side panels and lifting.

ChemWell should be installed on a sturdy, level, surface capable of supporting the instruments weight (45 kg) safely. The instrument should be surrounded by the following clearances: 46cm on each side, 117cm on top, 15cm front, and 18cm back. ChemWell requires no fastening to the bench top.

The quality of washing often affects the validity of test results. To assure adequate washing:

Perform periodic dispense volume repeatability checks as described in this manual.

Rinse the wash head and probe after use.

Handle and store the wash head carefully to prevent damage.

Use the prime cycle before each wash.

Watch the instrument to see that the probe and wash head dispense is functioning properly.

Be sure to run a sufficient number of controls in each assay. If controls are not within their acceptable limits, or if you suspect incomplete or non-uniform washing, disregard test results.

Do not operate the instrument if the pressure is unstable or if the probe or wash head probes are damaged.

IMPORTANT OPERATING PRECAUTIONS!


4

1.2.2 General Safety Summary Review the following safety precautions to avoid injury and prevent damage to this instrument or any products connected to it. To avoid potential hazards, use this instrument only as specified.

WARNING

Only qualified personnel should perform service procedures. Contact your dealer to arrange factory training.

1.2.3 To Avoid Fire or Personal Injury Use Proper Power Cord. Use only the power cord specified for this product and

certified for the country of use.

Ground the Product. This product is grounded through the grounding conductor of the power cord. To avoid electric shock, the grounding conductor must be connected to earth ground. An optional method is to attach a ground strap from the external grounding terminal on the rear panel of the instrument to a suitable ground such as to a grounded pipe or some metal surface to earth ground. See Figure 1.3.4.

Observe All Terminal Ratings. To avoid fire or shock hazard, observe all ratings and markings on the instrument. Consult this manual for further ratings information before making connections to the instrument.

Do Not Operate Without Covers. Do not operate this instrument with covers and panels removed.

Use Proper Fuse. Use only the fuse type and rating specified for this instrument.

Avoid Exposed Circuitry. Do not touch exposed connections and components when power is present.

Do Not Operate With Suspected Failures. If you suspect there is damage to this instrument, have it inspected by a qualified service person.

Provide Proper Ventilation. Refer to the installation instructions for details on installing the product so it has proper ventilation.

Do Not Operate in Wet/Damp Conditions.

Do Not Operate In An Explosive Atmosphere.

Keep Instrument Surfaces Clean and Dry.

WARNING

The operation of ChemWell may involve the use of biohazardous material. Refer to your owners manual for biohazard warnings.

!

!


5

1.2.4 Safety Terms and Symbols The following terms appear in this manual:

DANGER indicates an injury immediately accessible as you read the marking. WARNING indicates an injury hazard not immediately accessible as you read the marking.

CAUTION indicates a hazard to property including the product. BIOHAZARD indicates biological agents that can cause disease in humans. Lab workers handling potentially infectious materials must use universal precautions to reduce the risk of exposure to these agents.

These symbols may appear on the product:

WARNING Risk of Shock

Protective Ground (Earth) Terminal

CAUTION Refer To Manual

BIOHAZARD Risk of Infection

FUSE: For continued protection against risk of fire, replace only with fuse of the specified type and current ratings. Disconnect equipment from supply before replacing fuse.

DANGER: Pinch points, sharp points, and moving parts - mechanisms may operate without warning.

!

DANGER

!


6

1.3 Layout Refer to the figures on the following pages for illustrations of the ChemWell and its components.

ChemWell Component Figure Number

ChemWell Exterior View A

NOTE: For clarity of illustration, the aerosol shield is not shown.

Figure 1.3.1

ChemWell Exterior View B Figure 1.3.2

Right Side Panel Figure 1.3.3

Rear Panel layout Figure 1.3.4

Sub-assemblies:

Plate X Mover Figure 1.3.5

Plate Y Mover

NOTE: Plate X and Y Movers are combined and are responsible for the plate carrier movement.

Figure 1.3.6

Rack Y Mover

NOTE: Responsible for the sample and reagent racks movement

Figure 1.3.7

Probe X Mover Figure 1.3.8

Probe Z Mover

NOTE: Probe X and Z Movers are combined and are responsible for the probe movement.

Figure 1.3.9

WARNING

Hazardous line voltages are present behind the AC cover and on the power supplies. Always disconnect the external AC power cable before servicing the instrument.

WARNING

The operation of ChemWell may involve the use of biohazardous material. Refer to the Owners Manual for biohazard warnings.

!

!


7

ChemWell Component (Continued) Figure Number

Washer

NOTE: Responsible for the aspiration and dispensing of liquids using the wash head.

Figure 1.3.10

Electronics Rack

NOTE: Holds the main boards (PCAs) and the power circuitry.

Figure 1.3.11

Pressure and Valve Bracket

NOTE: Controls the fluid flow of the washer.

Figure 1.3.12

Syringe Pump

NOTE: Controls the fluid flow of the probe.

Figure 1.3.13

Photometer

NOTE: Houses the lamps, filters, and is responsible for photometric readings.

Figure 1.3.14

Bottles

NOTE: Holds liquids for the washer system.

Figure 1.3.15

ChemWell Coordinate System

NOTE: Explains the coordinate system for ChemWell

Figure 1.3.16


8


9


10


11


12


13

Figure #1.3.8

Serial #s 2900- 1782

Serial #s 2900- 1783 to current


14

Probe Z Mover

Figure #1.3.9


15


16


17


18


19


20


21

1.4 Technical Specifications

Technical Specifications for ChemWell Overall Typical throughput up to 200 endpoint reactions per hour or 170 kinetic reactions

per hour Typical reaction volume 200 uL or less Dimensions 86cm W x 51cm L x 40cm H, approx. weight = 45kg Reagent and Sample Dispensing Capabilities dilutions, predilutions, dispensing single or multiple reagents Pumps two syringe pumps, sized: 50 uL and 2.5 mL Probe 316 stainless for maximum reagent compatibility, level sensing Minimum and maximum volume

2 uL - 1.95 mL

Precision


22

Technical Specifications for ChemWell Minimum system Pentium/133 MHz, 32 MB RAM, 20 MB free HD, VGA

monitor, serial port, Windows 95/98 or Windows NT 4.0 Recommended system Pentium/333 MHz, 32 MB RAM, 20 MB free HD, SVGA

graphics and monitor, serial port, CD drive, Windows 95/98, Windows NT 4.0

Main menu options Patients, New Job, Job, Setup Secondary menu options create/edit protocols, import/export assays, data, etc., Control,

Run, Setup Calculation modes absorbance, single standard, factor, fixed time kinetics,

kinetics by standard or factor, multi-calibrator point-to-point, linear regressions, log-logit, cutoff by absorbance or standard, and more

Self monitoring modes lamp, bottle volumes, filters, pressure, vacuum, mechanical function, and more

QC options store control data, print Levey-Jennings or QC range plots, calculate SDs

Serial port RS232 output only, 9600 Baud, 1 start bit, 8 data, 1 stop, no parity, no handshake, serial cable provided

Power Voltage range 100-250VAC Frequency range 50-60Hz Power maximum 160W Installation category CAT II Environmental Conditions for Safe Operation Indoor use

Altitude up to 2000 m

Temperature 5C to 40C 1

Humidity 80% for temperatures up to 31C decreasing linearly to 50% humidity at 40C.

Mains supply voltage fluctuations not to exceed 10% of the nominal voltage

Recommended Environmental Conditions Recommended operating temperature

18-35C

Recommended operating humidity

less than 85%

Certifications NRTL listed

CE marked

Design and instrument specifications are subject to change without notice. 1 Although it may be safe to operate in these conditions, it may not be suitable for the performance of your tests. Check with your supplier.


23

2. PRINCIPLES OF OPERATION

2.1 System Control / Electronics Rack ChemWell has three large printed circuit boards which regulate and control the system. All three boards are mounted to the top of the electronics rack, easily accessed by lifting the top cover of the instrument. On the underside of the electronics rack (visible only after the back cover of the instrument has been removed) are the two switching power supplies and associated junction boards. See Figures #1.3.11 Electronics Rack, 2.2 Main PCB Connections, 2.3 Coprocessor PCB Connections, 2.4 Daughter PCB Connections, 2.5.8 DC Junction PCB Connections, and 2.7 Back of Electronics Rack.

The board on the far left as you face the front of the instrument is Main (2900-100). This board contains one of the two Z180 microprocessors. The other microprocessor can be found on the Coprocessor (2900-200) board, which is the middle of the three boards. The Coprocessor board has a direct bus interface to the board on the right, known as the Daughter (2900-300).

The functions and communications between these boards are described in the next sections on the following pages.


24

2.2 What Main Controls

External interface to computer (RS-232 Serial Interface)

Interface to Syringe Pumps (RS-232 Serial Interface)

Position Detection and control of:

o Probe Z

o Probe X

o Rack 1

o Rack 2

Probe / Coil Heating

Probe Level Sensing

CSI/O Interface to Co-processor


25

2.3 What Coprocessor Controls Photometer

Filter wheel

Lamps (via control of power supply 2)

Bus interface to daughter board

Power Supply #2

CSI/O to Co-processor

Daughter

Photometer


26

2.4 What Daughter Controls Position Detection and control of:

Wash head Plate X Plate Y

Plate Mixer

Plate Heater, Temperature Sensor, External Temperature Sensor

Pressure / Vacuum control

Pump Pressure and Vacuum Sensors

Valves

Rinse

Wash

Bleed

Pressure Ctrl

Bottle Liquid Level Sensors


27


28

2.5 All Junction Boards This section describes these additional boards, their dedicated roles, and their locations.

The Probe Sensor and Heater Control PCB control the fluid sensing and heat control for the coil block and probe. This board mounts to the top of the coil block.

The probe X board controls the sideways movement of the probe. This board is mounted on the left side of the probe X bracket under the electronics rack.


29


30

The Probe Z board controls the up-down motion of the probe. This board interfaces with the Probe Sense and Heater Control board located inside the probe housing.

The Rack Y Mover coordinates the motion of Rack 1 (reagent rack) and Rack 2 (sample rack). It is located on the rear of the Rack Mover Assembly.

The Photometer Junction board is the junction point for the Photometer mechanism. The board is located on the Photometer Module.


31

The Photometer PCB optically reads the light emitted by the lamp after passing through the plate. It is located inside the Photometer housing.

The LED Display shows the status of various states of the mechanisms. See troubleshooting for a full outline of what each light means. The board is located behind the Probe Shield.

The DC Junction board is the distribution center for DC power for the instrument. It is located under the electronics rack.


32

The Plate Mover coordinates the Plate X-axis, Plate Y axis movements, the Plate Carrier incubation, mixing, fan, and wash movements. It is located to the right and under the Plate Cover. Note: Plate Carrier connection is currently not used. The extra two-position connector with two orange wires should not be plugged into the board.

The Relay board turns the second power supply off. It is mounted inside the electronics rack.


33

2.6 Main and Coprocessor, How They Communicate

The cable from J8 of the Main board (2900-100) to J12 of the Coprocessor board (2900-200) interconnects the two processors and allows them to communicate. This connection uses a version of the Z180s CSI/O (Clocked Serial I/O). When a command is received from the PC by Main, the firmware on the Main board will determine if the command must be executed by the Coprocessor. This is the case, for example, when sending a command such as !WPRI, Wash Prime. Since both the wash head and the pressure system are controlled by the Coprocessor, it is responsible for executing this command. Using the CSI/O connection, Main will forward this command to the Coprocessor and wait for it to be completed. When the command is complete, the message will be sent from the Coprocessor to the Main board via the CSI/O connection and then echoed by Main back to the PC using the serial port.

In addition to the commands originating from the PC, Main and Coprocessor communicate to coordinate a variety of functions. This communication is transparent and the ChemWell can be treated as a unified instrument. The only time when it may become necessary for the service technician to examine the CSI/O connection is if the processors are unable to communicate at startup. How to diagnose and solve this problem is covered in the troubleshooting section.

The Coprocessor board and the Daughter board should be considered one unit. The Z180 microprocessor on the Coprocessor board directly controls the peripherals on the Daughter board by means of the bus interconnect. The 26-pin right-angle connectors directly tie together the data and address buses of the two boards.

2.6.1 Firmware versus Software The internal software, usually referred to as the firmware, is responsible for the direct control of the mechanisms and other systems. The firmware is actually divided into two parts; one for each of the two Z180 processors inside ChemWell. The communication between the processors is described above. The firmware is programmed into two EPROMs (UV-Erasable Programmable Read Only Memory) and installed on the two microprocessor boards. The firmware Main is for the Main board (2900-100) and Cop for the Coprocessor (2900-200). The firmware revision will be printed on the EPROM labels.


34

The software refers to the Microsoft Windows program running on the external PC. This is the program that interacts with the user and controls the ChemWell.

Fundamentally this software breaks down all operations into a set of commands which the instrument can execute. For an explanation of these commands refer to the Section on Troubleshooting.

The version of the software can be identified by selecting Help/About... from the ChemWell pull-down menu. In this dialog you will see a message in the format Version x.xx / Build yyy. The Build Number is the primary means of identifying the software version. Also, see section 3.1.3 Reports.

2.7 Power Supply

WARNING

The power supplies contain high voltage and can cause injury. Do not operate the instrument with the back cover removed

See also: System Overview Diagram in Appendix B

ChemWell uses two switching power supplies, both of which are mounted in the lower portion of the electronics rack. The power supplies should require no maintenance or adjustment in the course of normal operation. To access the power supplies, unplug the instrument and remove the four screws which attach the back cover of the instrument.

!


35

Facing the back of the instrument, you will see a small box at the right side containing the power receptacle and the main power switch. (Note that ChemWell has no voltage select switch as the power supplies are auto switching.) Inside this box are the main fuses for the instrument. See Fuse Replacement for more information. Two sets of AC lines originate from inside this box. One set goes directly to the first power supply and the other goes to the relay board. The first power supply converts AC power to provide DC +12V, +5V, and +24V to the instrument. It is always operating when the instrument is turned on.

The DC output from the first power supply connects to the adjacent DC Junction Board using a 13-wire header. This board distributes the DC voltage and has connections for the Main, Coprocessor, Daughter boards as well as the syringe pumps and internal lamps. See Appendices for the layout. Only one of J56 or J54 will be connected to the pumps and J10 (+24VDC) powers the internal lamps. The three connectors J50, J51, and J52 provide +12VDC, +5VDC, and two grounds to each of the three main boards. The +5V supply should be between +4.9V and +5.25V and the +12V supply between +11.5V and +12.25V depending on the current load.

The second power supply is turned on and off via the relay junction board, which controls the AC input power using a 12VDC relay. The relay is controlled by a ULN2003A (U9C) driver on the Coprocessor board. This second supply provides the lamp and photometer operating voltage. The lamps are wired directly to this supply to minimize voltage variation. Lamp supply voltage is approximately +6VDC. This supply also produces +/- 14-15VDC which is routed to the Coprocessor board at J6. Components VR2, VR3, C37, and C39 regulate these voltages to +/- 12VDC which are used to operate the photometer circuits. See Principles of Operation of the Photometer for more information.

2.8 Watchdog Circuit All valves, motors and pumps are powered through the major boards. The Main board provides +12 volts through an IRF9530A MOSFET Q1A, which is in turn driven by the re-triggerable one-shot comprised of C14, R5, and U12A. The microprocessor re-triggers U16A at intervals smaller than the one-shot period. If the microprocessor system crashes or otherwise malfunctions, U12A will time out and shut down the motors (rack and probe) and coil heating power by turning off Q1A. If this occurs, LED D3 will be turned off to provide a visual indication.

The Daughter board provides +12V to its systems through a Relay LR2, which is in turn driven by the re-triggerable one-shot comprised of C27, R17, and U13A. The microprocessor re-triggers U13A at intervals smaller than the one-shot period. If the microprocessor system crashes or otherwise malfunctions, U13A will time out and shut down the valves and pumps by turning off Relay LR2. If this occurs, the middle bar of a seven segment LED on the Daughter board will be turned off to provide a visual indication on the Daughter board.


36

2.9 Motion Control Refer also to section 3.5 Motor Control for general information on motion sensing.

Racks Two bidirectional DC motors, under microprocessor control, turn 3/8-16 machined stainless steel lead screws via neoprene belts and nylon pulleys. One motor is dedicated to Y motion of the rack 1 (reagent), the other to the Y motion of the rack 2 (sample). Attached to the lead screw is an encoder wheel which has ten holes that create pulses when passing between an IR LED and a phototransistor creating tachometer pulses provided to microprocessor. These tachometer pulses are provided to the microprocessor at the CLK1 input and CLK2 input at 8254 U5.

Micro switches positioned at extremes of rack movements disable the motor drivers U22 via NAND gates U18 when the travel limits are reached. Two switches additionally indicate the home positions to the microprocessor. LEDs D1 and D2 provide visual feedback to the service technician of the tach pulses and home positions. The Rack Junction PCB holds the motor drivers. The index pulse buffers, and limit logic, are on the Main PCB. The rack junction connects to Main via 10 pin ribbon cable and two wire power cable.

Plate Two bidirectional DC motors, under microprocessor control, turn 3/8-16 machined stainless steel lead screws via a 55 tooth 40 DP timing belt and two 27 tooth 40 DP timing pulleys. One motor is dedicated to X motion of the plate carrier, the other to the Y motion of the plate carrier. Each drive pulley attached to the motor has an encoder wheel with ten holes which create pulses when passing between an IR LED and a phototransistor, providing tachometer pulses to the microprocessor at the CLK1 input and CLK2 input at 8254 U16. Phototransistors, positioned at extremes of the plate carrier movements, disable the motor drivers U7 and U11 via NAND gates U10 and U18 when the travel limits are reached. Two of these phototransistors additionally indicate the home positions to the microprocessor. LED D1 on the Daughter board provides visual feedback to the service technician of the tach pulses and home positions. The plate mover junction PCB holds the motor drivers. The Daughter PCB holds the index pulse buffers, limit logic, and connects to the main PCB via a 26-pin ribbon cable and power. Additionally, phototransistors generate X and Y axis index pulses to position the plate carrier with regard to the photometer.

Mixer (Shaker) Mixing in wells is accomplished by shaking the Plate Carrier Y-axis platform. The platform is supported by 4 flexible natural latex rubber mountings and is attached to the underside of the platform (a motor driven counterweight). Spinning the counterweight causes the platform to shake on its mountings with proportional intensity. Microprocessor control of the shaker motor via U7 on the coprocessor PCB allows for adjusting the single mix/shake intensity setting. Adjust the mixing using Telix command, !MSPDxxxx. Adequate mixing should shake 250uL of reagent and sample vigorously, but without splashing up. Speeds 0030 to 0050 should cover the range.


37

Probe X

A bidirectional DC motor, under microprocessor control, pulls a slide via a nylon belt and nylon pulleys. The motor is dedicated to X motion of the probe. Probe X has a two-phased encoder wheel which creates pulses when passing between a reader, providing tachometer pulses to the microprocessor at the NMI input and PB4 input at 8255 U9. The one phase of the index pulley is used by the software to determine direction and accurately track the probes position. The probe-X junction board contains some jumpers that should not be adjusted. Another phototransistor, positioned toward the right side of the probe X movement, is used to verify and if necessary, reset the probe count additionally. Another phototransistor indicates the home position to the microprocessor. LED D1 on the Main board provides visual feedback to the service technician of the tach pulses and home positions. The Probe X Junction PCB holds the motor driver. The main PCB holds the limit logic and connects to the Probe X Junction PCB via a 10-pin ribbon cable and power.

Probe Z A bidirectional DC motor, under microprocessor control, turns a 1/4-20 machined brass lead screw via neoprene belt and nylon pulley. The motor is dedicated to the vertical Z motion of the probe. Probe Z uses a shuttle with an attached aluminum code wheel that has index holes which create pulses when passing between an IR LED and a phototransistor, providing tachometer pulses to the microprocessor at the CLK0 input at 8254 U5. There is a phototransistor positioned at the extreme of the probe Z movements. It will disable the motor drivers U27 via NAND gate U18 when the travel limits are reached.

Additionally other phototransistors indicates the home positions to the microprocessor. LED D2 provide visual feedback to the service technician of the tach pulses and home positions. The Probe Z Junction PCB holds the motor driver and interfaces to Probe Sense board. The main PCB holds the index pulse buffers, limit logic, and connects to the Probe Z Junction PCB via a 10-pin ribbon cable.

Washer One bidirectional DC motor, under microprocessor control, turns a 3/8-16 machined stainless steel lead screw via neoprene belt and nylon pulley. The motor is dedicated to vertical Z motion of the wash head. The pulley has several index holes which create pulses when passing between an IR LED and a phototransistor, providing tachometer pulses to the microprocessor at the CLK0 input at 8254 U16. Phototransistors, disables the motor driver U11 via NAND gate U10 when the travel limits are reached. Travel limits are indicated by encoder flag slot attached to the washer shuttle. The 7-segment LED on the daughter board provides visual feedback to the service technician of the tach pulses and home positions. The plate mover junction PCB contains the motor drivers and connects to the daughter board via a 26-pin ribbon cable. The daughter PCB holds the index pulse buffer, and limit logic.

2.10 Plumbing

2.10.1 Pressure and Vacuum Solid-state sensors P1 and P2 on the Daughter board are amplified at U6 and U8 and time division multiplexed via U9 into V/F converter U14, then measured at 8254 timer U2. This provides feedback to the microprocessor for coordinating pump activity and displaying measured pressure and vacuum levels. The vacuum pump is driven by U3 and MOSFET Q2A. The valves are driven by U3 and U15.


38

2.10.2 Pumps and Valves Refer to Figure # 1.3.12 for pictorial and Appendix E for Fluid System Schematic diagram. A diaphragm pump and valves, under microprocessor control via the daughter PCB, are switched on and off to generate pressure or vacuum as necessary. Two electromechanical valves, the pressure control valve and the bleed valve, are used to control and maintain the pressure in the systems wash and rinse bottles. The pressure control valve, controlled by U3, is an intermittent duty valve which regulates the pressure in the wash and rinse bottle.

When energized, the valve closes, causing pump pressure flow to be diverted from ambient into the check valve and into the bottles and sensor. The bleed valve allows the instrument to relieve pressure during standby and when power is removed.

The bleed valve is a continuous duty valve, also controlled via U3, and is continuously closed during any pressure cycle. It also serves as an emergency pressure relief valve in the event of power failure or microprocessor crash and opens when pressure is no longer required such as in standby or when timed out. A check valve, oriented to allow flow to the bottle side only, assures that the pressure is maintained on the bottle side of the system. Pressure is sensed by the circuit of P2 and U8.

Pressure build up occurs in the wash and rinse bottles simultaneously, producing positive wash and rinse fluid pressure in the wash and rinse lines of the system (see Appendix E). Two intermittent duty valves called the Rinse Valve and Wash Valve, which are normally closed, are pulsed open via U3 on the Daughter Board. The valves are used to regulate the flow of rinse and wash fluid to the washer head by precisely timed openings.

When the control valve is open to ambient and the pump is running, the pressure will not change and only vacuum will be produced by the pump. The vacuum side of the pump is connected to the waste bottle. Vacuum is monitored by microprocessor control by monitoring the output of solid state pressure sensor circuit P1 and U6 on the Daughter PCB and switching the pump on and off accordingly. Vacuum is not regulated but is monitored. Refer to Error Messages, Status Indicators, and Plumbing- Bottle Level Sensing.

2.10.3 Wash System Dispense The pump produces 34.5 kPa air pressure at the top of the wash and rinse bottles. The fluid is forced up from the bottle but is stopped by a solenoid pinch valve. The valve is opened for a precise interval under microprocessor control to allow fluid to flow into the dispense cavity of the wash head, where it is distributed to stainless steel capillary tubes and then into eight microwells.

Aspirate A vacuum is applied to the waste bottle any time the pump runs. The pump is connected to a fine hydrophobic aerosol filter then connected to the waste bottle. The filter prevents liquid from entering the pump. The waste bottle also connects to the aspirate cavity of the wash head. The vacuum is distributed across stainless steel capillary tubes thereby aspirating the contents of eight microwells simultaneously from the plate.


39

2.10.4 Draining The probe empties and flushes in the wash cup. Liquid exits the wash cup through tubing that drains into a waste trough underneath the rack mover. From there liquid drains to the drain bottle by gravity. The washer aspirates liquid from the plate into the waste bottle. The wash trough drains, by gravity, into the Waste Trough.

2.11 Bottle Fill Level Sensing The signal BOTLV is fed through a voltage divider to stainless steel wire probes mounted in the caps of the wash, rinse, and waste bottles. The signals are time division multiplexed via U9 into V/F converter U14 and the resulting frequency appears at the CLK2 input of 8254 timer U2. The microprocessor reads a change in frequency which corresponds to a change in conductivity of the material between the probes. Refer to 1.3.3 and 1.3.15.

2.12 Syringe Pumps The syringe pump is an OEM component of the ChemWell consisting of two syringe drives and one valve drive which are capable of independent operation through its own microcontroller board. The pump module has its own internal command set, and communications between the syringe pump and the ChemWell Main PCB occur serially via a 3 wire cable connected to J2 on the Main Board.

The valve has four possible positions that connect any two ports at right angles. The valve is used in only two of the four positions allowing the 2.5mL syringe to connect to either the priming tube or the interconnect tube.

Priming fluid from the prime bottle is introduced into the system by rotating the shear type valve to connect between the 2.5mL syringe and the priming tube, then drawing down on the syringe to aspirate prime fluid from the bottle. A third port, on the top of the valve, is unused.

Volume pipetting is produced by displacements generated in two syringes: 2.5 mL and 50 uL. The 2.5 mL syringe is used for large reagent volumes and the 50 uL syringe is for volumes less than 30 uL. The 50 uL syringe is attached to a T fitting, and does not require a valve. The syringes are connected by an interconnect tube between the valve (2.5mL) and T-fitting (50mL), and this is coupled to the pipetting system by a feeder line between the T-fitting (50mL) and a coupled line into the heat block/probe assembly.

See Figure #1.3.13.

2.13 Photometer The photometer consists of a mechanism with its own electronics and additional calibration and analog to digital conversion circuitry on the Coprocessor PCB. The mechanism contains the light sources, filters, detectors and electronics. Light from four tungsten-xenon lamps passes downward though four wells in the sample plate and the samples they contain. Inside the sealed box, under the read wells, is a rotating filter wheel and four photodiode detectors. The filter wheel contains eight interference filters of various wavelengths, and is speed controlled to approximately 3 rotations per second under software control.


40

While in motion, as each filter passes in front of the photodetector, an infrared optical switch triggers a sampling of the peak voltage by the Coprocessor board. The four voltages are then fed to four comparators which each compare the sampled voltage to the output of an exponential capacitor decay circuit. The pulse at the output of the four comparators enables individual 16 bit counters in two 8254 programmable timers. The counts are read by the microprocessor and this completes the analog to digital conversion.

The photo detector output is proportional to the intensity of the light, whereas the width of the positive phase of the comparator output is proportional to the absorbance. The resistance across the log cap (RC decay) determines the base of the log (10 for absorbance) and is used to adjust the low-end absorbance calibration (gain). Another four potentiometers are used to adjust the high-end absorbance (offset) of each channel.


41

2.14 Computer Connections The PC communicates with ChemWell via the serial port on the back of the instrument. The connector is a standard 9-pin serial port and the cable from the instrument to the PC is a standard Null Modem Cable. (Null Modem Cable is a cable made with a female 9 pin connect to another female 9 pin connector.) In this type of cable the RX/TX (receive and transmit) and the CTS/RTS lines are crossed. The serial communication is at 19,200bps, 8 data bits, no parity, and 1 stop bit.

Internally, the serial port is connected to the Main board (2900-100). The microprocessor on this board is responsible for communication with the PC and the Coprocessor and for routing commands within the ChemWell.


42

2.15 Computer Software Interface

2.15.1 Explanation of terms Each of these terms is explained in more detail in the Owners Manual.

Patient Database....Stores complete patient records including name, address, and doctor information.

Sample Protocol .....Procedure for aspirating and dispensing reagents or samples.

Wash Protocol ........Procedure for washing wells.

Method ...................Calculation modes and parameters for an assay.

Assay......................A test, made up of protocols, parameters, and options necessary to run a test.

Panel ......................A group of assays that are frequently run together.

Job Also known as a worklist, it contains the list of patients (or patient IDs) and assays to run.


43

2.15.2 Navigating the software When you first start ChemWell you will be asked to log in with a username. At present this is used only for record keeping as this username will be printed in reports. It is not necessary to enter a password.

ChemWell uses the standard Windows 95/98 Windows NT 4.0 controls, windows, and dialogs. If you are unfamiliar with these controls and how to use them, please refer to your Windows documentation. All of the ChemWell functions are available from the pull-down menus at the top of the program. Some of the more common functions are also available from the main menu.

2.15.3 ChemWell status window This window displays the current status of the instrument including the temperature and the status of the waste, wash, and rinse bottles. Also shown are the currently loaded racks and plate. The Functions button will start the Instrument Functions dialog described in the Owners Manual. The Wash Wells will wash all twelve rows of the plate currently loaded in the instrument. Use the New Wells button to tell the software that you have inserted a new plate or set of wells. All wells will be marked as available. The software automatically keeps track of which wells in the plate have been used. To manually edit this setup, or to mark certain wells as not available, click the Edit Plate button.

Note: The Wash Wells and New Wells functions are also available from the Run menu.

2.16 Instrument Functions This dialog gives you easy access to some of the more common instrument functions. To open it, go to the View menu and select ChemWell functions. The following options are available:

Note: The Start of Day and End of Day functions are also available from the Run menu.

Start of day .............Prepares the instrument to be run by turning on the photometer lamps, washing the probe, and priming the wash system.

End of day ..............Prepares the instrument to be shut down at the end of the day by turning off the photometer lamps, washing the probe, and rinsing the wash system.

Reset ......................Homes all of the mechanisms and reinitializes the syringes.

Standby ..................Parks the probe and turns off the pressure system.

Park probe..............Moves the probe to the center wash station.

Wash probe ............Washes the probe.

Prime wash.............Primes the wash system with the solution in the wash bottle.

Prime Syringes .......Primes the syringes with fluid from the prime bottle.

Prime rinse .............Primes the wash system with the solution in the rinse bottle.

Wash Wells ............Washes all twelve rows of the plate.

Heat to 37C...........Raises the coil and plate temperature to 37C.

Temp Off ................Turns the coil and plate temperature off.


44

3. TROUBLESHOOTING In this section, each subassembly or component group is discussed, and possible problems and solutions are outlined.

3.1 Service Tools

3.1.1 Test Mode When servicing the instrument it is often necessary to be able to send commands directly to the ChemWell. This can be done by selecting Telix Mode from the View menu. This mode displays the communication with the instrument and allows the commands to be typed in and executed. For an explanation of the ChemWell commands see Service Tools- Software Service Commands.

Caution: It is possible to damage the instrument by sending incorrect commands.

3.1.2 Status Indicators Main Board There are two 7 Segmented LEDs:


45

Probe Sense Board

LED status indicators on the Probe Sense PCB show whether or not the heat is on. LED D11 shows the heat for the heater coil, LED D8 shows the probe heat.

Coprocessor Board

LED D10 shows the status of power supply, and should always be on.

Daughter Board

LED D12: Plate Y slot indexing. LED D13: Plate X slot indexing.

LED 7 Segment

Plate Mover Junction LED D14: status indicator of the thermistor on the Plate heater.

LED D15: currently not in use.

LED Display Board:


46

3.1.3 Reports The ChemWell software includes a number of functions intended to make it easier to diagnose problems in the field. For example, there are several files and reports which the end-user can send back to the service center. At the top of every report printed by ChemWell is a message in the form: ssss/v.vv bbb/xxxx/yyyy/ppp-ddd, where

ssss indicates............................................ Instrument serial number v.vv indicates............................................. Software version number bbb indicates ............................................. Software build number * See note below xxxx indicates............................................ Main firmware revision yyyy indicates............................................ Coprocessor firmware revision ppp indicates ............................................. Primary filter (if the report is for a single

assay) ddd indicates ............................................. Differential filter (if the report is for a single

assay) *Note: If a set of parenthesis with another v.vv and bbb appear, you are viewing a data file with a different version than the data file was created under.

3.1.4 Updating the default reports The ChemWell software includes a set of default report templates including a template for each mode and for various special reports. The user can edit these default reports, but the software contains a way to restore them to their original form at any time. This may become necessary if the user-edited report templates contain errors which prevent them from being used. From the Setup menu of the software, select Preferences and then Default Reports. The Default Reports Setup dialog will be shown. Select the button Restore/Update all default report templates to restore all of the standard templates. It is recommended that custom reports be created by first copying one of the default reports to a new name. For more information on report templates please see the Report Template Specification in the Owners Manual.

3.1.5 System Info Report The system info report provides information on the computer that is running the ChemWell software. To access this report, select System Info from the Help menu of ChemWell.

The Windows version section will report information regarding the Windows installation. In Windows 95 the SP section will report the system revision.

CAUTION: There are several known problems with the versions of Windows 95 prior to B which do affect ChemWell. If the user has one of the earlier versions of Windows 95 the system should be upgraded.

ChemWell has been tested with Microsoft Windows 95B, 95C, 98, Windows NT 4 Workstation, Windows NT 4 Server, and Microsoft Windows 2000 Professional, Beta 3.


47

The Processor section will show information regarding the computer itself. The MHz speed of the computer is not available under Windows 95/98. The Memory section will report the total amount of physical and virtual memory. There should be at least 8MB (8096K) of physical memory. The total virtual (Page) memory should be at least twice the physical memory. If it is not, the user should change the virtual memory settings under the Windows Control Panel.

3.1.6 Using ChemWell Pack ChemWell Pack (CWPack) is a supplemental stand-alone program designed to ease the transfer of various ChemWell data files (assays, jobs, racks, data, etc.). Because the functionality of ChemWell Pack will eventually be integrated into the main ChemWell program, CWPack has been kept intentionally simple and inflexible. Contact your dealer if you do not have CWPack on your computer. There are two basic operations that CWPack can perform, packing and unpacking.

Packing files To pack a group of files, perform the following steps:

Using the File Management functions within ChemWell, export all files to be transferred to an empty directory.

Start CWPack by selecting on the Windows Start Menu -> Programs -> ChemWell -> ChemWell Pack. There is a checkbox option: Unpack files, and two directory/file name entry boxes: ChemWell Export Directory and Package File Name.

Make sure the Unpack files option is not checked.

ChemWell Export Directory always defaults to a:\chemwell. If you exported your files to a different directory, enter the name of that directory here.

Package File Name always defaults to a:\chemwell\CWPack.pck. If you would like the pack file to go into a different directory, or have a different name, enter that information here.

Press the button labeled Start.

CWPack will now process the ChemWell export files into a pack file. When it is done, the status window will report Pack Successful!.

You may now transfer and/or email the .PCK file to it destination.

Unpacking files To unpack a packed file, perform the following steps.

Start CWPack by selecting on the Windows Start Menu -> Programs -> ChemWell -> ChemWell Pack. There is a checkbox option: Unpack files, and two directory/file name entry boxes: ChemWell Export Directory and Package File Name

Make sure the Unpack files option is checked.


48

ChemWell Export Directory always defaults to a:\chemwell. Enter the name of a new or empty directory here. Do not enter the name of your ChemWell data directory here.

Package File Name always defaults to a:\chemwell\CWPack.pck . Enter the name of the file to be unpacked here.

Press the button labeled Start.

CWPack will now process the packed file and put the files into the directory you specified. When it is done, the status window will report Unpack Successful!.

You may use the File Management functions within ChemWell to import the data files.

Additionally, once CWPack has been run at least once, you can double-click on a .PCK file from within Windows Explorer, and CWPack will start automatically with the Unpack files option already checked, and the name of the .PCK file already in the Package File Name entry. In the ChemWell Export Directory box, enter the directory name of your choice.

3.1.7 Instrument Report With the computer connected to the instrument and the instrument turned on, select Instrument Setup from the Setup menu of ChemWell. Once the software has gathered the necessary information from the instrument the Print Report button will be enabled. This report displays several vital statistics regarding the instrument and the configuration stored in the computer.

For an explanation of these functions see the following.

Communications ComPort .................................................... the name of the por

Documents

Analette Manual Service Rev. D 2-6-08