Amino Acid Sequence Comparison between FGL2 and Claudin-1 Proteins in Hepatitis

By: Nicolle A. Rosa School: Levittown Baptist Academy Mentors: Dorielys Valentin and Karoline Rios

Outline

• Introduction

• Methods

• Results

• Conclusions

Introduction: Hepatitis

• Inflammation of the liver

• Infectious viral disease

• May be caused by toxins

• Could be acute or chronic

lookfordiagnosis.com

Introduction: Hepatitis B

• Transmitted mostly by infectious body fluids

• Preventable by vaccination

• Associated with fibroleukin prothrombinase

hephelp.net

Introduction: FGL2

• Causes blood to clot

• Leads to blockages in the vessels

• Causes a death of tissue in the liver

genecards.org

Introduction: Hepatitis C

• Most chronic form of hepatitis

• No vaccine has been developed

• Transmitted by blood to blood contact

• Associated with claudin-1 protein

trialx.com

Introduction: Claudin-1

• Involved in the maintenance of the cell’s structure

• Required for the virus to enter the cell

• Acts when the virus bounds to the cell

ghr.nlm.nih.gov

Objectives

• Determine the percentage of conserved amino acids between species.

• Infer phylogenetic trees to indicate the evolutionary changes between species and their amino acid sequences.

Methodology

Methodology

BLAST

ClustalW2

GeneDoc

MEGA5

Species selected for Comparison for Hepatitis B:

Bos taurus Sus scrofa Mus musculus

biopix.es

Methodology

m.forocoches.com arabrapto.blogspot.com

Rattus norvegicus Salmo salar Nomascus leucogenys

discoverlife.org es.wikipedia.org animaldiversity.ummz.umich.edu

Equus caballus Loxodonta africana Ornithorhynchus ananitus

dbird.us en.wikipedia.org usuarios.multimania.es

Gallus gallus

ncbi.nlm.nih.gov

Species selected for Comparison for Hepatitis C:

Methodology

Macaca mulatta Cricetulus griseus Sus scrofa

cienciamia.net midlandhamsterclub.co.uk globedia.com

Rattus norvegicus Bos taurus Ovis aries

ratremover.com fr.wikipedia.org factspage.blogspot.com judywoods.dial.pipex.com

Oryctolagus cuniculus Xenopus laevis Gallus gallus

guillermobustamante.com.mx combinacionanimal.blogspot.com

Danio reiro

misterpez.com

Genedoc Results for Hepatitis B

Genedoc Results for Hepatitis C

0%

2%

4%

6%

8%

10%

12%

LGNDE

Comparative Graph of Amino Acids for Hepatitis C

Comparative Graph of Amino Acids for Hepatitis C

0%

2%

4%

6%

8%

10%

12%

14%

ILAVM

Maximum Phylogenetic Tree for Hepatitis B

Minimum Phylogenetic Tree for Hepatitis B

Maximum Phylogenetic Tree for Hepatitis C

Minimum Phylogenetic Tree for Hepatitis C

Conclusions for Hepatitis B

• The amino acids are 9% conserved between the compared species.

• The graph shows that the most highly expressed amino acid is leucine.

• The maximum phylogenetic tree shows that Equus caballus and Loxodonta africana are the most similar while Salmo salar the least.

• In the minimum phylogenetic tree, the most similar species are Homo sapiens and Nomascus leucogenys, while Rattus norvegicus the least similar.

Conclusions for Hepatitis C

• The amino acids are 26% conserved between the compared species.

• The graph shows that the most highly expressed amino acid is isoleucine.

• Both the maximum and minimum phylogenetic trees show that Bos taurus and Ovis aries are the most similar while Sus scrofa the least.

References

• http://www.sciencedaily.com/releases/2007/02/070227213105.htm

• http://www.eurekalert.org/pub_releases/2003-07/uot-ric070203.php

• http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0002139/

• http://www.webmd.com/hepatitis/hepb-guide/hepatitis-b-topic-overview

• http://www.medicinenet.com/hepatitis_c/article.htm

•Dorielys Valentin •Karoline Rios • Institute of Mathematics: Saturday Academy •Dr. Juan Arratia •Dr. Sudhir Kumar •Allan Filipski •Wanda Rodriguez, Program Coordinator

Acknowledgements

Any Question?