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ALyS series Achieves much number of NK cells and a high NK cell ratio Specially designed for human NK cells ALyS 505NK - AC & EX for human NK cells +81-22-399-6608 1-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan Protect from light NK Cell Culture Medium Cat.No. 01400P10 F or R&D use only 00XX00 0000.00 8 1000 mL ALyS505NK-EX IL-2 () ALyS505NK -AC without IL-2 ALyS505NK -AC1000 with IL-2 1000 IU/mL For activation ALyS505NK -EX without IL-2 ALyS505NK -EX1000 with IL-2 1000 IU/mL For expansion +81-22-399-6608 1-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan Protect from light NK Cell Culture Medium Cat.No. 01600P02 F or R&D use only 00XX00 0000.00 8 200 mL ALyS505NK-AC IL-2 () culture bag product available

ALyS series ALyS 505NK-AC EX · ALyS 505NK-AC &EX for human NK cells +81-22-399-6608 1-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan Protect from light NK Cell Culture Medium Cat.No

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Page 1: ALyS series ALyS 505NK-AC EX · ALyS 505NK-AC &EX for human NK cells +81-22-399-6608 1-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan Protect from light NK Cell Culture Medium Cat.No

ALyS series

Achieves much number of NK cells anda high NK cell ratio

Specially designed for human NK cells

ALyS 505NK-AC & EXfor human NK cells

+81-22-399-66081-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan

Protect from light

NK Cell Culture Medium

Cat.No. 01400P10

For R&D use only

00XX000000.008

1000 mL

ALyS505NK-EXIL-2 ( –)

ALyS505NK-AC

without IL-2

ALyS505NK-AC1000

with IL-2 1000IU/mL

For activation

ALyS505NK-EX

without IL-2

ALyS505NK-EX1000

with IL-2 1000IU/mL

For expansion

+81-22-399-66081-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan

Protect from light

NK Cell Culture Medium

Cat.No. 01600P02

For R&D use only

00XX000000.008

200 mL

ALyS505NK-ACIL-2 ( –)

culture bag productavailable

Page 2: ALyS series ALyS 505NK-AC EX · ALyS 505NK-AC &EX for human NK cells +81-22-399-6608 1-16-16 Seikan, Aoba-ku Sendai, 982-0262 Japan Protect from light NK Cell Culture Medium Cat.No

NIPRO CORPORATION3-9-3, Honjo-Nishi, Kita-ku, Osaka531-8510, JAPAN

URL: http://www.cstimedia.comE-mail: [email protected]

1-16-16 Seikaen, Aoba-ku, Sendai, 982-0262, JAPAN

(P)PET bottle (CB)Culture Bag

Product Name Description VolumeCode(CSTI / NIPRO)

200mL(P)

200mL(P)

1,000mL(P)1,000mL(CB)

1,000mL(CB)1,000mL(P)

01600P02 / 87-554

01610P02 / 87-555

01400P10 / 87-55601400C10 / 87-558

01410C10 / 87-55901410P10 / 87-557

ALyS505NK-AC

ALyS505NK-AC1000

ALyS505NK-EX

ALyS505NK-EX1000

NK cell culture medium

NK cell culture medium [for activation](without IL2)

[for activation](with IL2 1000 IU/mL)NK cell culture medium

[for expansion](without IL2)

NK cell culture medium[ for expansion](with IL2 1000IU/mL)

ALyS 505NK-AC&EX

Achieve a highly-pure NK cell rate.It contains human derived proteins.Any other animal derived component free.

* ALyS505NK-AC1000 / ALyS505NK-EX1000

5

10

15

0 5 10 14Culture days

Num

ber o

f cel

ls(×

107 ce

lls)

ALyS505NK- AC&EX

competitor G

Fig.1 Robust proliferation

5

0

10

20

15

A B C

Num

ber o

f NK

cells

(×

107 ce

lls)

competitor G

Fig.2 Number of NK cells at the day 14th

ALyS505NK- AC&EX

20

0

40

100

60

80

cell A cell B cell C

Phen

otyp

e Ra

tio(%)

ALyS505NK- AC&EX

competitor G

Fig.3 Ratio of NK cells and T cells

T cellsNK cells

T cellsNK cells 20

0

40

100

60

80

0 2 4 6 8 10E/T ratio

Cyt

otox

ity(%)

Fig.4 Cytotoxic effect of NK cells

ALyS505NK- AC&EX

Performance data of ALyS505NK-AC&EX*

Serum-free medium for NK cells

ALyS505NK-AC and -EX are serum-free mediumoptimal for NK cell therapy.

※ For Research use only.