• Almost all plants are photosynthetic autotrophs, as are some bacteria and protists– Autotrophs generate their own organic matter through

photosynthesis– Sunlight energy is transformed to energy stored in the

form of chemical bonds– Why are plants green?

(a) Mosses, ferns, andflowering plants

(b) Kelp

(c) Euglena (d) Cyanobacteria

THE BASICS OF PHOTOSYNTHESIS

Light Energy Harvested by Plants & Other Photosynthetic Autotrophs

6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2

• Photosynthesis is the process by which ______________organisms use light energy to make sugar and oxygen gas from carbon dioxide and water

AN OVERVIEW OF PHOTOSYNTHESIS

Carbondioxide

Water Glucose Oxygengas

PHOTOSYNTHESIS

• The Calvin cycle makes:

LightChloroplast

Lightreactions

Calvincycle

NADP

ADP+ P

• The light reactions:

AN OVERVIEW OF PHOTOSYNTHESIS

Chloroplasts: Sites of Photosynthesis

• Photosynthesis– Occurs in chloroplasts, organelles in certain

plants– All green plant parts have chloroplasts and carry

out photosynthesis• The leaves have the most chloroplasts• The green color comes from chlorophyll in the

chloroplasts• The pigments absorb light energy

• In most plants, photosynthesis occurs primarily in the leaves, in the chloroplasts

• A chloroplast contains: – stroma, a fluid – grana, stacks of thylakoids

• The thylakoids contain chlorophyll– Chlorophyll is the green pigment that captures

light for photosynthesis

Photosynthesis occurs in chloroplastsPhotosynthesis occurs in chloroplasts

• The location and structure of chloroplasts

LEAF CROSS SECTION MESOPHYLL CELL

LEAF

Chloroplast

Mesophyll

CHLOROPLAST Intermembrane space

Outermembrane

Innermembrane

ThylakoidcompartmentThylakoidStroma

Granum

StromaGrana

• Chloroplasts contain several pigments

Chloroplast Pigments

– Chlorophyll a – Chlorophyll b – Carotenoids

Figure 7.7

Different pigments absorb light differently

Excitedstate

e

Heat

Light

Photon

Light(fluorescence)

Chlorophyllmolecule

Groundstate

2

(a) Absorption of a photon

(b) fluorescence of isolated chlorophyll in solution

Excitation of chlorophyll in a chloroplast

Loss of energy due to heat causes the photons of light to be less energetic.

Less energy translates into longer wavelength.

Energy = (Planck’s constant) x (velocity of light)/(wavelength of light)

Transition toward the red end of the visible spectrum.

e

Cyclic Photophosphorylation • Process for ATP generation associated with

some Photosynthetic Bacteria• Reaction Center => 700 nm

Ph

oto

n

Ph

oto

n

Water-splittingphotosystem

NADPH-producingphotosystem

ATPmill

• Two types of photosystems cooperate in the light reactions

Primaryelectron acceptor

Primaryelectron acceptor

Electron transport chain

Electron transport

Photons

PHOTOSYSTEM I

PHOTOSYSTEM II

Energy forsynthesis of

by chemiosmosis

Noncyclic Photophosphorylation • Photosystem II regains electrons by splitting

water, leaving O2 gas as a by-product

2 H + 1/2

Water-splittingphotosystem

Reaction-center

chlorophyll

Light

Primaryelectronacceptor

Energyto make

Electron transport chain

Primaryelectronacceptor

Primaryelectronacceptor

NADPH-producingphotosystem

Light

NADP

1

23

How the Light Reactions Generate ATP and NADPH

• Two connected photosystems collect photons of light and transfer the energy to chlorophyll electrons

• The excited electrons are passed from the primary electron acceptor to electron transport chains– Their energy ends up in ___________________

In the light reactions, electron transport In the light reactions, electron transport chains generate ATP, NADPH, & Ochains generate ATP, NADPH, & O22

• The electron transport chains are arranged with the photosystems in the thylakoid membranes and pump H+ through that membrane– The flow of H+ back through the membrane is

harnessed by _____________________– In the stroma, the H+ ions combine with NADP+

to form ________________

Chemiosmosis powers ATP synthesis in the light reactions

• The production of ATP by chemiosmosis in photosynthesis

Thylakoidcompartment(high H+)

Thylakoidmembrane

Stroma(low H+)

Light

Antennamolecules

Light

ELECTRON TRANSPORT CHAIN

PHOTOSYSTEM II PHOTOSYSTEM I ATP SYNTHASE

• A Photosynthesis Road Map

Chloroplast

Light

Stack ofthylakoids ADP

+ P

NADP

Stroma

Lightreactions

Calvincycle

Sugar used for

Cellular respiration Cellulose

Starch

Other organic compounds

Review: Photosynthesis uses light energy to make food molecules

Light

Chloroplast

Photosystem IIElectron transport

chains Photosystem I

CALVIN CYCLE Stroma

Electrons

LIGHT REACTIONS CALVIN CYCLE

Cellular respiration

Cellulose

Starch

Other organic compounds

• A summary of the chemical processes of photosynthesis