Alcohol has the largest death rate out of all other drugs

Disorders caused by alcohol have increased over the past 6 years

Null Hypothesis: the ethanol exposure will have no effect on the short term memory of

Drosophila melanogaster.

Alternate Hypothesis: The Drosophila exposed to ethanol before training will have impaired memory and the flies exposed to alcohol after training will not have damaged memory. The control group will form short term memory

The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in

drosophila.

AlcoholShort Term Memory

Short-term memory is stored in the α/β mushroom body neurons

Olfactory training

This diagram shows how alcohol effects the brain

This graph shows the results of the two different groups. The chpd flies’ short-term memory didn’t last as long as the control group

Comparison Between Control and chpd Drosophila

Need

Knowledge Base

Literature Review

Purpose

Hypothesis

Moore, 98Moore, 98

LaFerriere, 07LaFerriere, 07

AbstractAbstract

GoesGoes

HereHere

The Heat Box

A model of the heat-box used to train short-term memory

Results

Time interval results

This diagram shows the process of short term memory in the brain

Deaths From Disease

Figure 1

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7Figure 8

The Effects of Alcohol on Short Term Memory in Drosophila

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control Alcohol Before Alcohol After

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Wild Type Drosophila

Control

N=950

Alcohol Before

N=800

Alcohol After

N=850

Exposed to Alcohol for 5 minutes

Trained using olfactory

Trained using olfactory

Trained using olfactory

Exposed to Alcohol for 5 minutes

Tested 5 hours laterTested 5 hours laterTested 5 hours later

Methodology

Results

Performance index used to see if learning occurred

Performance Index: (Correct-Incorrect)-.5/.5 x100

Testing Chamber

Flies are lowered between the familiar and unfamiliar scents and are given 4 minutes to choose

Training Chamber

Flies are shocked while simultaneously being being pumped an odor.

Drosophila Learning Without Middle Flies

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Figure 9 Figure 10

Figure 11 Figure 12

Results are put through the performance index with the flies that moved to the unfamiliar scent are correct and those that stayed in the middle or moved to the familiar scent were incorrect

Results are put through the performance index with the flies that moved to the unfamiliar scent are correct and those that moved to the familiar scent were incorrect and those that stayed in the middle were discounted

Data supported part of the alternate hypothesis which stated that the flies exposed to ethanol before training will have impaired memory.

Effects of alcohol on long term memory.Effects of sleep deprivation on short-term memory.Effects of resveratrol on short-term memory.Effects of alcohol on short-term memory in ADHD flies

Bayard, Max. "Alcohol Withdrawal Syndrome - March 15, 2004 - American Family Physician." American Academy of Family Physicians. 2004. 25 May 2009 <http://www.aafp.org/afp/20040315/1443.html>.

Ericson, Nels. "Substance Abuse:The Nation’s Number One Health Problem." 2001. U.S. Department of Justice. <http://www.ncjrs.gov/pdffiles1/ojjdp/fs200117.pdf>.

Farr, Susan. "Drinking for just eight weeks impairs learning and memory in mice." Science, Industry and Business: the innovations-report. 15 May 2009 <http://www.innovations-report.com/html/reports/life_sciences/report-45402.html>.

Grant B, Harford TC, Dawson DA, et al. Prevalence of DSM-IV alcohol abuse and dependence, United States 1992. Alcohol Health & Research World 18(3). 1994.

Laferriere, Holly, and Douglas J. Guarnieri. "Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster." (2008). Genetics. <http://www.genetics.org/cgi/content/full/178/4/1895/>.

Miller, Loren L., and Michael P. Dolan. "SpringerLink - Journal Article." SpringerLink Home - Main. 1973. <http://www.springerlink.com/content/u0161w1781274g83/>.

Moore, Monica, and Jim DeZazzo. "Ethanol Intoxication in Drosophila:Genetic and Pharmacological Evidence for Regulation by the cAMP Signaling Pathway." (1998). <http://www.northwestern.edu/neurobiology/faculty/allada2/gbbpapers/MooreHeberlein98.pdf>.

The number of trials for drosophila exposed to alcohol could have been higher and possible human errors.

Ethanol beaker

Air pump

Alcohol Exposure

Exposure chamber

The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in drosophila.

Results demonstrated that alcohol exposure before and after had similar results.

When graphed with out the undecided flies results appeared similar with the before and after groups varying by a slight percent

Discussion

Limitations Conclusion

Future Studies

Bibliography

Flies are exposed to 200 Proof ethanol for 5 minutes before or after training

Figure 13