Alcohol has the largest death rate out of all other drugs

disorders caused by alcohol have increased over the past 6 years

Null Hypothesis: the ethanol exposure will have no effect on the short term memory of

Drosophila melanogaster.

Alternate Hypothesis: the Drosophila exposed to ethanol will have impaired

short term memory, and the Drosophila who are not, will not

The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in

drosophila.

Alcohol

Short Term Memory

http://www.bcm.edu/labs/davis/researchprojects.htm

Olfactory training

http://www.jpp.krakow.pl/journal/archive/1203_s4/gfx/rys16_2.jpg

Holly LaFerriere 2007

http://www.northwestern.edu/neurobiology/faculty/allada2/gbbpapers/MooreHeberlein98.pdf

Comparison between Control and chpd Drosophila

Control and chpd

Moore, 98

Need

Knowledge Base

Literature Review

Purpose Hypothesis

The Effects of The Effects of Alcohol on Alcohol on Short-Term Short-Term Memory in Memory in Drosophila Drosophila MelanogasterMelanogaster

Moore, 98

LaFerriere, 07

Testing Chamber

The Effects of Alcohol on Short Term Memory in Drosophila

50.5

7.810.8

-30

-20

-10

0

10

20

30

40

50

60

70

80

control Alcohol Before Alcohol After

Groups

Perf

orm

an

ce I

nd

ex

Wild Type Drosophila

ControlAlcohol Before

Alcohol After

Exposed to Alcohol for 5 minutes

Trained in olfactory method with one

minute shock increments every

10 minutes

Trained in olfactory method with one

minute shock increments every

10 minutes

Trained in olfactory method with one

minute shock increments every

10 minutesExposed to Alcohol for

5 minutes

Training Chamber

Tested 5 hours laterTested 5 hours later

Tested 5 hours later

Methodology

Results

Performance index used to see if learning occurred

Data Supported the Alternate Hypothesis

Effects of alcohol on long term memory.

Bayard, Max. "Alcohol Withdrawal Syndrome - March 15, 2004 - American Family Physician." American Academy of Family Physicians. 2004. 25 May 2009 <http://www.aafp.org/afp/20040315/1443.html>.

Ericson, Nels. "Substance Abuse:The Nation’s Number One Health Problem." 2001. U.S. Department of Justice. <http://www.ncjrs.gov/pdffiles1/ojjdp/fs200117.pdf>.

Farr, Susan. "Drinking for just eight weeks impairs learning and memory in mice." Science, Industry and Business: the innovations-report. 15 May 2009 <http://www.innovations-report.com/html/reports/life_sciences/report-45402.html>.

Grant B, Harford TC, Dawson DA, et al. Prevalence of DSM-IV alcohol abuse and dependence, United States 1992. Alcohol Health & Research World 18(3). 1994.

Laferriere, Holly, and Douglas J. Guarnieri. "Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster." (2008). Genetics. <http://www.genetics.org/cgi/content/full/178/4/1895/>.

Miller, Loren L., and Michael P. Dolan. "SpringerLink - Journal Article." SpringerLink Home - Main. 1973. <http://www.springerlink.com/content/u0161w1781274g83/>.

Moore, Monica, and Jim DeZazzo. "Ethanol Intoxication in Drosophila:Genetic and Pharmacological Evidence for Regulation by the cAMP Signaling Pathway." (1998). <http://www.northwestern.edu/neurobiology/faculty/allada2/gbbpapers/MooreHeberlein98.pdf>.

The number of trials for drosophila exposed to alcohol could have been higher and possible human errors.

Performance Index: (Correct-Incorrect)-.5/.5 x100

Ethanol beaker

Air pump

Alcohol Exposure

Exposure chamber

The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in drosophila.

Results demonstrated that exposure to alcohol impaired short term memory

Discussion

Limitations Conclusion

Future Studies

Bibliography