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imagination at work
GE Healthcare
Data File 18-1124-09 AG Chromatography systems
ÄKTAexplorer chromatography systemsÄKTAexplorer™ chromotography systems (Fig 1) are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples. The method wizard and optimized system configurations, allow rapid media screening, method scouting, method optimization, and scale-up experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required. Systems can be used at flow rates up to 100 ml/min (pressure 10 MPa) to ensure minimized run times at maximum flow rates or at 10 ml/min (pressure 25 MPa) for applications routinely using columns that generate higher backpressures. Preparation times are minimized while accuracy and reproducibility of results are assured.
Key advantages of using ÄKTAexplorer include:
Fast, simple start to every purification
• method wizard for easy programming
• automatic evaluation and reporting
Simple column selection suited to each purification
• comprehensive column library
Fast, systematic method optimization
• automatic media screening
• variable run parameters in automated scouting schemes
No manual titration of buffers
• accurate, automatic BufferPrep covers a broad pH range
Flexibility from laboratory to production
• easy system modification
• fast, trouble-free transfer of methods to production scale
Fig 1. ÄKTAexplorer 100, from the ÄKTAexplorer system series.
ÄKTAexplorer system is a member of ÄKTAdesign™, a range of liquid chromatography systems for academic and industrial research laboratories. ÄKTAdesign systems are controlled by UNICORN™, a control, evaluation, and reporting platform that also controls production systems at kg scale. UNICORN ensures quick, simple communication between systems and users and meets the stringent control and data handling procedures of modern laboratories. Optimized methods are transferred easily to production scale.
Figure 2 shows how ÄKTAexplorer 100 can be used to minimize separation time without losing resolution by using high flow rates on modern high-performance media.
2 Data File 18-1124-09 AG
Fast and easy start to purificationÄKTAexplorer systems are entirely controlled by UNICORN software, enabling a fast and easy start to purification. Users are able to create complete purification schemes for a range of biomolecules by using the method Wizard. This makes ÄKTAexplorer extremely easy to use and speeds up routine purifications. It reduces the need for expertise in programming, in chromatography, and in time-consuming literature searches, as all this knowledge is built into the system.
The UNICORN method Wizard provides a unique combination of flow configuration and method support for all chromatographic techniques including gel filtration, anion and cation exchange, hydrophobic interaction, affinity, and reversed phase chromatography. Using input parameters such as components in use, flow rate, sample volume, or gradient, the method Wizard builds up the method to suit your needs (Figs 3-5). The method Wizard eliminates time-consuming and repetitive method programming and uses column volume as a programming base for easy use with any size column.
Routine runs quickly optimizedTo quickly establish routine runs, the method Wizard supports programming of method scouting (Fig 6). These scouting methods make it easy to systematically vary run parameters in repeated, automated procedures, reducing optimization times.
Time (min)
10
20
3040
60 ml/min
0 4 6 10
Abs
orba
nce
Fig 2. Automated scouting for maximum flow rate using ÄKTAexplorer 100.
Run
Selected method
Select technique Select
column
Fig 3. Starting a new method is easy.
Fig 4. Create a new method in less than three minutes, using the method wizard.
Fig 5. Simple input of chromatographic variables.
Column: RESOURCE™ S 6 mlSample: protein standards
Fig 6. Automatic scouting during purification development and optimization. Example shows scouting for flow rate, sample volume, and gradient length.
Data File 18-1124-09 AG 3
Fig 7. Automatic, user-defined evaluation and report procedures.
Methods are automaticallyadapted to your new column
All column specifications
Create your own library
Choose any column
Fig 8. Comprehensive column library ensures optimum running conditions.
Accurate result comparisonsAll data, including running conditions, are stored permanently by UNICORN within the run log (e.g., flow, pressure, UV, conductivity, temperature). This makes it easy to compare runs (e.g., Fig 7) and determine a cause for any deviations that may be seen.
Column knowledge built-inÄKTAexplorer ensures every column is run optimally and is protected from damaging events such as overpressure.
The user selects a prepacked column from the extensive UNICORN controlled column library (Fig 8) and ÄKTAexplorer automatically sets the optimal running parameters for that column (e.g., column volume, flow rate, and pressure limit) into the method. The user adjusts running conditions (if required) by simple method editing. New columns can be added to the library and will be run in the same secure way.
Fast, systematic method optimizationMethod optimization and process development on chromatographic systems often involves systematic variation of key run parameters, such as media selection, binding and elution buffers, or sample loading flow rates. UNICORN control system includes scouting schemes within each general method that eliminate the need for creating new methods for each set of parameters. These scouting schemes make it easy to systematically vary any parameter in repeated automated runs, without creating a large number of individual methods (Fig 6).
The method wizard supports the most common scouting procedures:
• Automatic media screening (Fig 9)
• Automatic eluent pH scouting for ion exchange chromatography (Fig 14)
mAU mS/cm
Column:RESOURCE RPC6.4/100
Column:µRPC C2/C18ST 4.6/100
Column:Sephasil PeptideC4 5 µm ST 4.6/100
Column:Sephasil PeptideC8 5 µm ST 4.6/100
0 10.0 20.0 30.0 min
Column:Sephasil PeptideC18 5 µm ST 4.6/100
Fig 9. Media screening with ÄKTAexplorer 10. Selection of reversed phase media for peptide purification. Sample: angiotensin mixture. Eluent system: 100 mM phosphate pH 8.0, CH
3CN 75%. All columns run at same linear
flow rate.
4 Data File 18-1124-09 AG
No manual titration of buffersThe BufferPrep function increases speed and the reliability of results by eliminating the time-consuming manual buffer preparation and titration usually needed for every pH change in chromatography.
The user selects the buffers from the buffer library and defines the pH required (Fig 10). BufferPrep prepares correct buffer pH on-line from four stock solutions (e.g., water, salt, buffer, acid/base) and controls the pH throughout the run, automatically compensating for changes in temperature and salt concentration. New buffer substances can be added to the buffer library and used by BufferPrep to create the required pH elution conditions.
The accuracy of pH is crucial in many separations (Fig 11). The accuracy of BufferPrep greatly increases the reproducibility and reliability of results from day to day by eliminating and correcting errors that occur when producing buffers manually.
BufferPrep is used for pH scouting in rapid method optimization. The method wizard makes programming of automatic pH scouting for ion exchange chromatography easy and safe. Figure 12 shows BufferPrep used on ÄKTAexplorer 100 at 60 ml/min to test seven pH conditions to find the optimal pH for binding and elution of a pure Fab fragment.
Flexibility and safety from laboratory to productionEasy system modificationsNew functions to enhance system capabilities or create new system configurations are easily added. Functions, consisting of hardware components and software instructions, are selected, installed, and implemented by the user. UNICORN controls and monitors the new functions in the same way as all other ÄKTAdesign components.
Select from buffer library
Fig 10. Simple programming for automatic buffer preparation.
pH 5.5
pH 4.8
pH 4.65
pH 4.5
pH 3.9
cytochrome c
Fig 11. Small changes in pH significantly change chromatographic results. Here a change of 0.15 units alters an elution profile.
250
200
150
100
50
0
2.0
mAU 280 nm mS/cm
80.0
60.0
40.0
20.0
4.0 6.0 8.0 Time (min)
START CONDITIONS
pH 7
Run 2Run 1 Run 3 Run 4
6.5 6 4.5
Run 4
5.5
Run 5
5
Run 6
4.5
Run 7
4
pH 4.5
pH 4.0
pH 5.0
pH 5.5
pH 6.0
pH 6.5
pH 7.0
RESOURCE S6 ml columnFab fragment
Fig 12. Automatic pH scouting on ÄKTAexplorer 100.
Data File 18-1124-09 AG 5
Fast, trouble-free transfer of optimized methods to production scaleUNICORN control system is used at operational scales from the laboratory bench to full production. UNICORN combines the flexibility needed for method and process development with the stringent requirements for commercial manufacture of biopharmaceutical products on production chromatography systems.
The same user interface and methods used to optimize a purification are applied in commercial manufacture with larger systems. This common control platform means quick and simple transfer from one stage of a project to the next. Figure 13 shows a 10-fold scale-up of a Phenyl Sepharose™ 6 Fast Flow column during process development of a Fab fraction purification, using an ÄKTAexplorer 100 and the same method template.
ÄKTAdesign supportUNICORN networksUNICORN control system has full networking capability, with high security and maximum compliance with regulatory needs. Network support allows control of any ÄKTAdesign system from any connected UNICORN workstation. System control and data access via a PC network is extremely easy
Column: Phenyl Sepharose 6 Fast Flow (high sub), XK 16/10, 20 mlSample: Fab fraction Samplevolume: 80 mlBufferA/BufferB: Buffer B + 1 M ammonium sulfate/ 50 mM sodium acetate pH 5.0System: ÄKTAexplorer 100
AU280 nm
2.00
1.00
0
100
50
Conductivity(mS/cm)
0 50 100 150 200 250Volume (ml)
AU280 nm
2.00
1.00
0
100
50
Conductivity(mS/cm)
0 500 1000 1500 2000 2500Volume (ml)
A B
Column: Phenyl Sepharose 6 Fast Flow (high sub), XK 50/20, 200 mlSample: Fab fractionSamplevolume: 800 ml BufferA/BufferB: Buffer B + 1 M ammonium sulfate/ 50 mM sodium acetate pH 5.0System: ÄKTAexplorer 100
AU280 nm
2.00
1.00
0
100
50
Conductivity(mS/cm)
0 50 100 150 200 250Volume (ml)
AU280 nm
2.00
1.00
0
100
50
Conductivity(mS/cm)
0 500 1000 1500 2000 2500Volume (ml)
A B
Fig 13. Direct scale-up on ÄKTAexplorer 100 from 20-ml to 200-ml column.
to use and gives a complete overview of operations. With defined access rights, operators connect to and control systems from any remote control or local PC. The user interface is exactly the same. Results can be automatically saved on a server and evaluation and generation of reports can be made locally or at remote PCs.
ÄKTAdesign serviceRegular, planned maintenance increases productivity by keeping a system in top working condition and minimizing downtime. A service agreement lets users budget for all service and maintenance costs, giving full cost control and eliminating unexpected expenses. Service agreements greatly simplify compliance with regulatory and quality demands.
Scheduled preventive maintenance carried out by qualified engineers and the built-in service diagnostics of ÄKTAdesign systems provides users with support to meet the requirements of Good Laboratory Practice (GLP). ÄKTAdesign systems are supported with a comprehensive range of after-sales service agreements that can be customized to individual needs. All ÄKTAdesign instruments are supported in the laboratory by our equipment specialists to minimize operational disruption and ensure functionality of ÄKTAdesign equipment.
6 Data File 18-1124-09 AG
ÄKTAexplorer Systems: Description and componentsFigure 14 shows some standard configurations of ÄKTAexplorer chromatography systems.
The standard functions available on all systems:
• BufferPrep on-line
• Automatic buffer selection (maximum eight inlets per pump)
• Automatic column switching/media scouting (maximum eight columns)
• Sample application
– fixed volume (100 µl to 2 ml) or partial loop fill (>100 µl)
– Superloop 10 ml, Superloop 50 ml, and Superloop 150 ml for samples 1 to 10 ml, 1 to 50 ml, and 1 to 150 ml, respectively
– via sample pump P-950 for larger volumes
ÄKTAexplorer 10 S10 ml/min, 25 MPa
B B
A2
A1
Pump
BufferPrep
Fractionation
Buffer Selection
Column Scouting
pH-C UV-Vis
Multiple Sample Injection
ÄKTAexplorer 100100 ml/min, 10 MPa
BufferPrep
Buffer Selection
B B
A2
A1
Pump
Fractionation
Flow Direction
Column Scouting
pH-C UV-Vis
Multiple Sample Injection
ÄKTAexplorer 1010 ml/min, 25 MPa
B B
A2 A1
Pump
BufferPrep
Fractionation
Buffer Selection
Column Scouting
UV-VispH-C
ÄKTAexplorer 100 Air 100 ml/min, 10 MPa
B B
A2
A1
Pump
BufferPrep
Fractionation
Buffer SelectionFlow Direction
Column Scouting
pH-C UV-Vis
Multiple Sample Injection
Air Sensor
Air Sensor
• Multiple wavelength detection (maximum three wavelengths simultaneously)
• pH/conductivity recorded on-line
• fraction collection options ranging from microplates to large vessels
• Automatic pump wash with column protection
All systems are supplied assembled and tested. Requiring minimal bench space, each system is mounted on a swivel platform for easy access to every side. Columns are snapped in place on the outside of the system, either individually or as groups held in column holders.
Fig 14. Representative flow diagrams for different ÄKTAexplorer Systems.
Data File 18-1124-09 AG 7
System componentsÄKTAexplorer systems have three main instrument components, a number of ancillary items, the option of a fraction collector and an autosampler (Fig 15). All instrument settings and functions are under direct control of UNICORN.
At every start-up, each instrument automatically performs a self-diagnostic test and calibrates appropriate settings. This ensures consistent performance over the long-term.
All wetted materials are inert, fully biocompatible, and resistant to all eluents commonly used in liquid chromatography, ensuring high recovery and no loss of biological activity.
Pumps provide reliable resultsThe main challenges of a chromatography system pump are to achieve accurate gradient formation over a broad flow rate range and avoid introducing air into the system. ÄKTAexplorer systems are equipped with either Pump P-901, which enables a wide flow rate range for both laboratory- and pilot-scale applications without changing pump modules or pump heads, or with Pump P-903, which operates at the lower flow rates and higher pressure required for running high-resolution analyses at laboratory scale. Figure 16 shows the flow rates and pressure ranges of the two pumps.
Monitor ph/C-900
Monitor ph/C-900
Monitor UV-900
Fig 15. The main components in every ÄKTAexplorer chromatography system.
Fraction collectorFrac-950
Autosampler A-900
Fig 16. Flow rate ranges and pressure ranges of P-900 pumps.
10
P-903
P-901
0.001 0.01Flow rate range (ml/min)
Pres
sure
ran
ge (M
Pa)
20
0.1 1 10 100
Monitor pH/C-900Monitor pH/C-900 is a combined monitor for accurate, online measurement of pH, conductivity, and temperature. The monitor records real-time information about chromatographic conditions. Monitor pH/C-900 provides an accurate response, coupled with high precision over a wide measuring range. This makes Monitor pH/C-900 an excellent choice for use in all chromatography techniques, from reversed phase chromatography with very low conductivity eluents to hydrophobic interaction chromatography in high salt solutions.
Monitor pH/C-900 consists of a control unit, an optional flow cell for conductivity and temperature, an optional flow cell with a holder for the pH electrode, and the pH electrode.
8 Data File 18-1124-09 AG
Monitor UV-900Monitor UV-900 is a high sensitivity, multi-wavelength UV-Vis monitor, recording up to three wavelengths simultaneously in the range 190 to 700 nm, which is the optimal detection range. The use of advanced fiber optics together with the unique flow cell design ensures a high signal-to-noise ratio for maximum performance. Maintenance costs are minimized by use of a unique Xenon flash lamp, giving a significant increase in lamp lifetime.
ÄKTAexplorer 100 chromatography systems include a UV-900 monitor with a 2-mm pathlength flow cell (internal volume 2 µl). The flow cell in ÄKTAexplorer 10 chromatography systems has a pathlength of 10 mm (internal volume 9 µl).
Ancillary equipmentStandardSample Pump P-960
Pump P-960, together with an IV-908 valve, is used for automatic multiple sample injection. The flow rate range (up to 50 ml/min), low pulsation, and pressure specification (up to 1 MPa) allow rapid sample application directly onto a column or via a Superloop. Large sample volumes can be applied smoothly and safely under the control of UNICORN. This function is standard in ÄKTAexplorer 100, ÄKTAexplorer 100 Air, and ÄKTAexplorer 10 S and optional in other systems.
Valves
Seven-port injection valves, INV-907, are used for sample application and reverse flow elution.
Eight-port fractionation valves are used for buffer selection (IV-908), automatic sample application, column switching and flow diversion to fraction collector, large fraction collection, or waste (PV-908).
Two switch valves work together with the P-900 pump in BufferPrep mode.
Mixer M-925
Mixer M-925 is a dynamic, single chamber mixer powered and controlled from Pump P-900. For optimal mixing in the entire flow rate range of each ÄKTAexplorer system, interchangeable mixing chambers are supplied. ÄKTAexplorer 100 includes 2, 5, and 12-ml chambers while ÄKTAexplorer 10 has 0.6 and 2-ml chambers.
OptionalFraction collector
Frac-950 (Fig 15) prevents sample loss across the entire flow rate range of ÄKTAdesign systems, from 0.001 ml/min to 100 ml/min, and the highest flexibility in the choice of collection mode. Fractions are collected in microplates (over 384 fractions, up to 2.5 ml per fraction), a variety of test tube sizes, or in 20 large vessels (up to 250 ml per fraction) or even funnels for unlimited fraction size. Collection is in volume or time mode and different fraction sizes can be collected during different stages of a separation.
Autosamplers
Autosamplers give you more time to work on other projects during the day, and increase throughput by enabling overnight purification runs. Depending on your needs for sample injection, Autosampler A-900 and A-905 for ÄKTAdesign systems offer a variety of alternatives.These autosamplers offer different balances between reproducibility and accuracy and allow flexible use of vials and microplates. Almost every type of vial with an o.d. of 12 mm or 7 mm can be used on Autosampler A-900. Autosampler A-905 handles standard and deep-well 96-well microplates, as well as 384 microvials. Sample loss is kept to a minimum or eliminated by the novel method of sample withdrawal. The Peltier cooling is incorporated in both autosamplers and effectively protects heat–sensitive biomolecules during sampling. Autosampler A-900 and Autosampler A-905 are directly controlled by UNICORN software.
Air sensors
Controlled through UNICORN, air sensors are used together with the UNICORN WATCH programming function to increase control over programmed events. For example, UNICORN is programmed to ensure that chromatographic elution begins only when completion of sample application has been detected by the air sensor. Large, variable sample volumes are applied with no risk of air entering the system.
Flow control
Motorized valves INV-907, IV-908, or PV-908 can be added to allow additional programmable functions such as automated reverse flow through a column or flow diversion.
Data File 18-1124-09 AG 9
AD-900 Analog/Digital Converter
AD-900 Converter is an analog/digital converter for connecting external instruments to UNICORN control software. The AD-900 Converter allows easier monitoring of signals received as voltage inputs from external sources such as fluorescence detectors, radioactivity monitors, refractive index monitors, or diode arrays.
ÄKTA 3D plus Kit
• Automatic multi-step purification of tagged proteins
• Save time and effort by purifing multiple samples in a single run
• Protect valuable target protein as proteases are removed
• Purify up to 50-mg proteins with greater than 90% purity
ÄKTA 3D plus Kit is aimed specifically at providing automated preparative purification of soluble His
6 (polyhistidine) - and
GST (Glutathione S-transferase) -tagged target proteins for structural genomics and proteomics applications. The ÄKTA 3D plus Kit is used together with ÄKTAexplorer 100 and includes a combination of hardware and software with a choice of different automated purification protocols that contain up to three chromatographic steps. It increases throughput by avoiding the need for manual steps after sample loading, purifying up to six samples in one automated run.
ÄKTA High Flow Kit
• Higher flow rate: 200 ml/min
• Lower back pressure: < 0.6 MPa
• Higher capacity
ÄKTA High Flow Kit contains the necessary components and software for reducing system backpressure and thereby allowing higher flow rates. This makes it a suitable setup for running larger columns and increasing the production capacity.
System specificationsÄKTAexplorer 10 systems
Flow rate range 0.001–10 ml/min
Pressure range 0–25 MPa
Pressure limits programmable upper and lower limit
ÄKTAexplorer 100 systems
Flow rate range 0.01–100ml/min
Pressure range 0–10 MPa
Pressure limits programmable upper and lower limit
Wavelength range 190–700 nm, 3 wavelengths simultaneously
Conductivity range 1 µS/cm–999.9 mS/cm (RPC – IEX – HIC gradients)
pH range 2–12
Solvent compatibility all commonly used chromatographic solvents
Size 500 mm × 460 mm × 620 mm (W × D × H)
Weight 75 kg
Operating dataPump P-903 series
P-903
Flow rate range 0.001–10 ml/min
Increment 0.001 ml/min
Internal volume < 600 µl/pump module
Pressure range 0–25 MPa (250 bar, 3625 psi)
P-901
Flow rate range 0.01–100 ml/min
Increment 0.01 ml/min
Internal volume < 1800 µl/pump module
Pressure range 0–10 MPa (100 bar, 1450 psi)
Pressure limits programmable upper and lower limit
Viscosity maximum 5cP
Monitor UV-900
Wavelength range 190–700 nm in steps of 1 nm, 3 wavelengths simultaneously
Bandwidth 4 nm
Wavelength accuracy ± 2 nm
Wavelength reproducibility ± 0.01 nm
Linearity < 2% deviation up to 2 AU at
260 nm with Uracil at pH 2
Noise* (at 230 nm) < 6 × 10–5 AU
Drift (at 254 nm) < 2 × 10–4 AU/h
Max. pressure 2 MPa (20 bar, 290 psi)
* Measured with water at 1 ml/min, time constant 1 s, 10 mm flow cell.
10 Data File 18-1124-09 AG
Operating data
Monitor pH/C-900
Conductivity range 1 µS/cm–999.9 mS/cm
Noise ± 0.5% of full-scale calibrated range
pH range 0–14 (specifications valid between 2–12)
Accuracy ± 0.1 pH unit, temperature compensated
Stability max 0.1 pH units deviation/10 h
Fraction collection
WithFrac-950
Flow rate range 0.001 ml/min–100 ml/min
Fraction capacity
Rack A 120 × 18 mm tubes 8 × 30 mm tubes
Rack B 240 × 12 mm tubes
Rack C 4 microplates (4 × 96 wells per plate) 8 × 30 mm tubes
Rack D 45 × 30 mm tubes
Rack E 80 × 30 mm tubes
Rack F 20 × 250 ml vessels
Rack G 30 funnels
Prep Mode Conversion Kit
WithFrac-901
Tube capacity 175 in Tube Rack 12 mm
95 in Tube Rack 18 mm (optional)
40 in Tube Rack 30 mm (optional)
With outlet valve for 7 large fractions (8 with Frac-901 disconnected)
Sample Pump P-950
Flow rate range 0.1–50 ml/min
Operating pressure 0–1.0 MPa
Viscosity 5 cP
10 cP at reduced flow rate
Autosampler A-900
Sample capacity 96 standard vials (1.5ml)
160 micro vials (0.5 ml)
Injection volume 5–1000 µl, flushed loop
1–500 µl, partial loop fill
1–475 µl, pick up
Injection valve switch time < 100 msec
Precision
Full loop injections rsd ≤ 0.3%
Partial loopfill injections rsd ≤ 0.5% for injection
volumes > 5 µl
µl pick-up injections rsd ≤ 1.0% for injection
volume > 5 µl
Sample viscosity 0–10 cP
A-900 with cooling
Cooling capacity > 11.5°C below ambient temperature (T) for 16 °C < T < 40°C
Typical cooling times 34 min from 23°C to 4°C
(at 45% relative humidity)
60 min from 32°C to 4°C
(at 35% relative humidity)
Programming through UNICORN control only
Autosampler A-905 for ÄKTA
Sample capacity
96-well microplate Direct filling or 96 microvials (low/high)
384-well microplate Direct filling or 384 microvials
48-vial adapter 48 vials
Injection volume 5–1000 µl, filled loop
1–500 µl, partial loopfill
1–475 µl, µl pick-up
Dispenser syringe 100 µl, 250 µl, 500 µl, and 1000 µl
(standard)
Injection precision ≤ 0.3% RSD filled loop
> 5 µl ≤ 0.5% RSD partial loopfill
> 5 µl ≤ 1.0% RSD µl pick-up
Programming Through UNICORN only
Cooling capacity Max. 12°C below ambient
temperature for working
temperatures between 16°C and 40°C
Dimensions (W × H × D) 280 × 440 × 400 mm
Analog/digital Converter AD-900
Voltage range ± 2V DC
Resolution 1 µV, 20 bit
Input impedance > 1 MV
Accuracy > ± 0.5 % or ± 0.05 mV
Max. input voltage ± 5 V
Power requirement 18–36 DC through the UniNet 2 connection
Dimensions incl. attachment (W x D x H) 45 x 175 x 85 mm
Weight 0.4 kg
Computer requirements
Pentium 4, 1.5 GHz
256 Mb RAM
500 Mb available hard disk (150 Mb to run UNICORN),
NTFS file system
CD-ROM drive
Windows™ 2000 or XP
Data File 18-1124-09 AG 11
Ordering informationProduct Code No.
ÄKTAexplorer 100 18-1112-41
ÄKTAexplorer 10 18-1300-00
ÄKTAexplorer 10 S 18-1402-00
ÄKTAexplorer 100 Air 18-1403-00
Frac-950 fraction collector complete with Rack A 18-6083-00
OR
Frac-920 fraction collector complete with 18 mm tube rack 18-1177-40
Autosampler A-900 18-1116-61
OR
Autosampler A-900, cooled version 18-1144-61 (both include 200 x 1.5 ml sample vials and caps)
ÄKTAdesign XT kit 18-6083-19 Includes UNICORN analysis module and tubings for connection of ÄKTAdesign systems to autosampler A-900
Air sensors
Air-900 control box, includes one UniNet 18-1121-22 2 cable, (controls up to 3 air sensors)
Purge valve (required for buffer inlet position only) 18-1126-33
Air sensor Air-925 (2.5 mm i.d.) 18-1121-24
Air sensor Air-912 (1.2 mm i.d.) 18-1121-23
Optional function valves (e.g., for reverse flow, flow diversions) (up to 3 valves of any type)
INV-907, including one UniNet cable 18-1108-40
IV-908, including one UniNet cable 18-1108-42
PV-908, including one UniNet cable 18-1108-41
Sample application
Superloop 10 ml (ÄKTAdesign), load 1–10 ml 18-1113-81
Superloop 50 ml (ÄKTAdesign), load 1–50 ml 18-1113-82
Superloop 150 ml, load 1–150 ml 18-1023-85
(requires union 1/16″ female – M6 male fitting, code no. 18-1112-57)
Additional items
UNICORN analysis module 18-1134-74
AD-900, including a UniNet cable 0.7 m, 18-1148-62 a Mini-DIN cable 1.5 m
ÄKTA 3D plus Kit 11-0014-53
ÄKTA High Flow Kit* 18-1154-85
UNICORN High Flow strategy and CD
(requires UNICORN v. 3.2 or higher)
Valve INV-907H
Valve kit PV-908-H for valve 2, 3, and 4
Instructions
* The kit is installed by a representative of GE Healthcare.
Related product literature
Product Code No.
Data Files
ÄKTApurifier Chromatography Systems 18-1119-48
Monitor UV-900 18-1111-17
Pump P-900 series 18-1119-49
ÄKTAprime plus 11-0034-88
UNICORN Control System 18-1111-20
Fraction Collector Frac-950 18-1153-57
Autosampler A-900/A-905 18-1177-97
imagination at work
GE, imagination at work and GE Monogram are trademarks of General Electric Company.
ÄKTAdesign, ÄKTAexplorer, ÄKTAprime, ÄKTApurifier, Drop Design, RESOURCE, Sepharose, and UNICORN are trademarks of GE Healthcare companies.
Any use of UNICORN software is subject to GE Healthcare Standard Software End-User License Agreement for Life Sciences Software Products. A copy of this Standard Software End-User License Agreement is available on request.
All third party trademarks are the property of their respective owners.
© 1999–2007 General Electric Company – All rights reserved. First published 1999.
All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.
GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Ltd Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Bio-Sciences KK Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan
www.gelifesciences.com/akta
GE Healthcare Bio-Sciences AB Björkgatan 30751 84 UppsalaSweden
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18-1124-09 AG 04/2007