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AGILENT SMALL MOLECULE LC COLUMNS OVERVIEW: A FAMILY OF PHASE CHOICES TO PERFECT EVERY SEPARATION Learn more at www.agilent.com/chem/discoverporoshell Choose the right LC column for your analysis with the LC Column and Sample Prep Navigator at navigator.chem.agilent.com ZORBAX Eclipse Plus ZORBAX StableBond InfinityLab Poroshell 120 SB-C18 InfinityLab Poroshell 120 SB-C8 ZORBAX Eclipse XDB InfinityLab Poroshell 120 EC-CN ZORBAX Extend-C18 InfinityLab Poroshell 120 HPH-C18 InfinityLab Poroshell 120 HPH-C8 ZORBAX Bonus-RP InfinityLab Poroshell 120 Bonus-RP InfinityLab Poroshell 120 PFP ZORBAX SB-AQ ZORBAX HILIC InfinityLab Poroshell 120 SB-Aq InfinityLab Poroshell 120 HILIC Polar Compounds High pH Best all around–exceptional peak shape, efficiency, resolution, and lifetime InfinityLab Poroshell 120 columns deliver exceptional HPLC efficiency, boosting performance for all instruments including older 400 bar and newer 1300 bar UHPLC systems You can trust the ZORBAX name for superior reproducibility and long-term stability Best for low pH mobile phases–great for method development High performance over a wide pH range Best lifetime and peak shape at high pH Alternative selectivity to alkyl, phenyl, cyano phases High sensitivity for LC/MS applications and recommended for EPA 1694 RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar Great for Method Development C18 (USP L1) C8 (USP L7) Phenyl-Hexyl (USP L11) PAH (USP L1) High performance and excellent peak shape with acids, bases, and neutrals. 1.9 μm, 1300 bar 2.7 μm and 4 μm, 600 bar 2.7 µm, 600 bar 2.7 µm, 600 bar 1.9 μm (HPH-C18), 1300 bar 2.7 μm and 4 μm, 600 bar 1.9 μm (PFP), 1300 bar 2.7 μm and 4 μm (PFP), 600 bar 1.9 μm (HILIC), 1300 bar 2.7 μm and 4 μm (HILIC), 600 bar RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar C18 (USP L1) High efficiency and long life at high pH–up to pH 11.5. Improve retention, resolution and peak shape of basic compounds. High sensitivity for LC/MS separations of peptides. Unique bidendate bonding and double endcapping provides high pH stability. RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar SB-C18 (USP L1) SB-C8 (USP L7) SB-C3 (USP L56) SB-Phenyl (USP L11) SB-CN (USP L10) High performance with acids, bases, and neutrals with superior lifetime at low pH. RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar Bonus-RP (USP L60) Polar-embedded to improve peak shapes; for basic compounds at low and mid pH. RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar C18 (USP L1) C8 (USP L7) Phenyl (USP L11) CN (USP L10) Good peak shape for basic, acidic, and neutral compounds with high performance over a wide pH range (pH 2-9). eXtra Dense Bonding and double endcapping help give this column a long lifetime. RRHD: 1.8 µm, stable to 1200 bar RRHT: 1.8 µm, 600 bar Proprietary phase ideal for polar compounds and high aqueous conditions. Exceptional lifetime at low pH– no endcapping. RRHD: 1.8 µm, stable to 1200 bar HILIC Plus is a HILIC column based on Eclipse Plus silica for excellent peak shapes. CH 3 CH 3 O O O O O CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si R R R R R R R 1 Si O OH OH R 1 Si O R 1 Si O CH 3 CH 3 O O O O O CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si CH 3 CH 3 Si O Si C18 C18 Silica Support Si O O O O PG R CH 3 CH 3 CH 3 Si CH 3 CH 3 CH 3 Si R 1 Si R 1 PG R R 1 Si R 1 PG R R 1 Si R 1 For Research Use Only. Not for use in diagnostic procedures. Information is subject to change without notice. © Agilent Technologies, Inc. 2017 Published in USA, June 30, 2017 5991-6240EN Unique-chemically modified for high pH stability InfinityLab Poroshell 120 EC-C18 InfinityLab Poroshell 120 EC-C8 InfinityLab Poroshell 120 Phenyl-Hexyl Start here for method development

AGILENT SMALL MOLECULE LC COLUMNS …...boosting performance for all instruments including older 400 bar and newer 1300 bar UHPLC systems You can trust the ZORBAX name for superior

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Page 1: AGILENT SMALL MOLECULE LC COLUMNS …...boosting performance for all instruments including older 400 bar and newer 1300 bar UHPLC systems You can trust the ZORBAX name for superior

AGILENT SMALL MOLECULE LC COLUMNS OVERVIEW:

A FAMILY OF PHASE CHOICES TO PERFECT EVERY SEPARATION

Learn more at www.agilent.com/chem/discoverporoshellChoose the right LC column for your analysis with the LC Column and Sample Prep Navigator at navigator.chem.agilent.com

ZORBAX Eclipse Plus ZORBAX StableBond

InfinityLab Poroshell 120 SB-C18InfinityLab Poroshell 120 SB-C8

ZORBAX Eclipse XDB

InfinityLab Poroshell 120 EC-CN

ZORBAX Extend-C18

InfinityLab Poroshell 120 HPH-C18InfinityLab Poroshell 120 HPH-C8

ZORBAX Bonus-RP

InfinityLab Poroshell 120 Bonus-RPInfinityLab Poroshell 120 PFP

ZORBAX SB-AQ

ZORBAX HILIC

InfinityLab Poroshell 120 SB-AqInfinityLab Poroshell 120 HILIC

Polar CompoundsHigh pH

Best all around–exceptional peak shape, efficiency, resolution, and lifetime

InfinityLab Poroshell 120 columns deliver exceptional HPLC efficiency,

boosting performance for all instruments including older 400 bar and newer 1300 bar UHPLC systems

You can trust the ZORBAX name for superior reproducibility and long-term stability

Best for low pH mobile phases–great for method development

High performance over a wide pH range

Best lifetime and peak shape at high pH

Alternative selectivity to alkyl, phenyl, cyano phases

High sensitivity for LC/MS applications and recommended for EPA 1694

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 bar

Great for Method DevelopmentC18 (USP L1)

C8 (USP L7)Phenyl-Hexyl (USP L11)PAH (USP L1)

High performance and excellent peak shape with acids, bases, and neutrals.

1.9 μm, 1300 bar 2.7 μm and 4 μm, 600 bar

2.7 µm, 600 bar 2.7 µm, 600 bar 1.9 μm (HPH-C18), 1300 bar2.7 μm and 4 μm, 600 bar

1.9 μm (PFP), 1300 bar2.7 μm and 4 μm (PFP), 600 bar

1.9 μm (HILIC), 1300 bar2.7 μm and 4 μm (HILIC), 600 bar

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 bar

C18 (USP L1)

High efficiency and long life at high pH–up to pH 11.5. Improve retention, resolution and peak shape of basic compounds. High sensitivity for LC/MS separations of peptides. Unique bidendate bonding and double endcapping provides high pH stability.

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 bar

SB-C18 (USP L1)SB-C8 (USP L7)SB-C3 (USP L56)SB-Phenyl (USP L11)SB-CN (USP L10)

High performance with acids, bases, and neutrals with superior lifetime at low pH.

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 bar

Bonus-RP (USP L60)

Polar-embedded to improve peak shapes; for basic compounds at low and mid pH.

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 bar

C18 (USP L1)C8 (USP L7)Phenyl (USP L11)CN (USP L10)

Good peak shape for basic, acidic, and neutral compounds with high performance over a wide pH range (pH 2-9). eXtra Dense Bonding and double endcapping help give this column a long lifetime.

RRHD: 1.8 µm, stable to 1200 barRRHT: 1.8 µm, 600 barProprietary phase ideal for polar compounds and high aqueous conditions.

Exceptional lifetime at low pH– no endcapping.

RRHD: 1.8 µm, stable to 1200 bar

HILIC Plus is a HILIC column based on Eclipse Plus silica for excellent peak shapes.

CH3

CH3

O

O

O

O

O

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

R

R

R

R

R

R

R1SiO

OH

OH

R1SiO

R1SiO

CH3

CH3

O

O

O

O

O

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

CH3

CH3

Si

OSi

C18

C18

Silica Support

SiO

O

O

O

PG R

CH3

CH3

CH3

Si

CH3

CH3

CH3

Si

R1

Si

R1

PG R

R1

Si

R1

PG R

R1

Si

R1

For Research Use Only. Not for use in diagnostic procedures.Information is subject to change without notice.

© Agilent Technologies, Inc. 2017Published in USA, June 30, 20175991-6240EN

Unique-chemically modified for high pH stability

InfinityLab Poroshell 120 EC-C18InfinityLab Poroshell 120 EC-C8InfinityLab Poroshell 120 Phenyl-Hexyl

Start here for method development