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You think it up. We knock it out.
IN THE FIELD OF MICEONE COMPANY STANDS OUT
Your source for C57BL/6 mouse knockout / knockin models
inGenious Targeting Laboratory, Inc.Phone: (631) 444-6848 • Fax: (631) 444-8839 • www.genetargeting.com
FOR MORE INFORMATION VISIT WWW.GENETARGETING.COMor
CONTACT KRISTEN TWIDY at [email protected]
Now you can see results of the finest methods in action with new online movies inCSH Protocols.
The November issue of CSH Protocols features methods for tracking RNA and protein molecules in both cells and organisms, and introduces new multimediacontent made possible by online presentation: movies showing examples of thesetechniques in action.
Read—and see—these newly featured protocols online at www.cshprotocols.org:
• Photoactivation-Based Labeling and In Vivo Tracking of RNAMolecules in the Nucleus
• Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
CSH Protocols offers cutting edge and classic protocols in molecular and cell biologyfrom laboratories worldwide, along with methods from Cold Spring HarborLaboratory’s renowned manuals and on-site courses. Free trials and discounted subscriptions are available for a limited time.
CSH Protocols is created by Cold Spring Harbor Laboratory Press in associationwith HighWire Press of Stanford University.
For pricing information or to request a free trial visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations)FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924
Subject Coverage
Antibodies
Bioinformatics/Genomics
Cell Biology
Chromatography
Computational Biology
DNA Delivery/GeneTransfer
Electrophoresis
Genetics
High ThroughputAnalysis
Imaging/Microscopy
Immunology
Laboratory Organisms
Molecular Biology
Neuroscience
Plant Biology
Polymerase ChainReaction (PCR)
Proteins and Proteomics
RNA Interference(RNAi)/siRNA
Stem Cells
Transgenic Technology
Cold Spring Harbor Protocols • Executive Editor: Dr. Michael RonemusISSN 1559-6095 / online, monthly • Available exclusively via institutional site license
A B
81 K –48 K –
35 K –
28 K –
19 K –
HA fusion proteins
A B C132 K
90 K
55 K
43 K
34 K
< OctA (FLAG®)- tagged fusion protein
Immunofl uorescence staining of methanol-fi xed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining. Antibodies tested include c-Myc (A-14): sc-789 (A) and c-Myc (9E10): sc-40 (B).
HA-probe (F-7): sc-7392. Western blot analysis of HA-tagged fusion proteins showing N-terminal HA-tagged JNK2 (A) and JNK1 (C) and C-terminal HA-tagged Daxx (B).
OctA-Probe (D-8)-G: sc-807-G. Western blot analysis of OctA (FLAG®)-tagged fusion protein from Sigma.
fusion protein tag antibodiesPRODUCT CAT# ISOTYPE APPLICATIONS
GFP (FL) sc-8334 rabbit IgG WB, IP, IF
GFP (T-19) sc-5384 goat IgG WB, IF
GFP (B-2) sc-9996 mouse IgG2a WB, IP, IF
His-probe (H-15) sc-803 rabbit IgG WB, IP
His-probe (H-3) sc-8036 mouse IgG1 WB, IP
c-Myc (9E10) sc-40 mouse IgG1 WB, IP, IF, IHC(P), FCM
c-Myc (9E11) sc-47694 mouse IgG2a WB, IP, IF, IHC(P)
c-Myc (A-14) sc-789 rabbit IgG WB, IP, IF, FCM
GST (Z-5) sc-459 rabbit IgG WB, IP
GST (B-14) sc-138 mouse IgG1 WB, IP
HA-probe (F-7) sc-7392 mouse IgG2a WB, IP, IF
HA-probe (Y-11) sc-805 rabbit IgG WB, IP, IF
OctA-Probe (D-8) sc-807 rabbit IgG WB, IP, IF, IHC(P)
OctA-Probe (H-6) sc-7787 mouse IgG1 WB, IP, IF, IHC(P)
GAL4 (DBD) (N-19) sc-729 rabbit IgG WB, IP
GAL4-TA (768) sc-429 rabbit IgG WB, IP
The Power to Question
© 2006-2007 Santa Cruz Biotechnology, Inc., all rights reserved. "Santa Cruz Biotechnology" and the Santa Cruz Biotechnology, Inc. logo are registered trademarks of Santa Cruz Biotechnology, Inc.
Santa Cruz Biotechnology, Inc. offers a wide range of antibodies to fusion protein tags.
Select HA, GST, His, GFP, GST, c-Myc and OctA tag antibodies are featured here.
See our web site www.scbt.com for our complete fusion protein tag antibody product
line. OctA-Probe fusion protein tag antibodies are raised against an eight amino acid
epitope tag identical to the FLAG® peptide sequence.
santa cruz biotechnology, inc.
fusion protein tag antibodies
SEE OUR WEB SITE AND NEW 2007 CATALOG FEATURING: 5512 new monoclonal antibodies
2472 new polyclonal antibodies
5082 new siRNAs
HEADQUARTERS
TEL: 831.457.3800
TOLL FREE: 1.800.457.3801
FAX: 831.457.3801
EMAIL: [email protected]
EUROPEAN SUPPORT
TEL: +49.6221.4503.0
TOLL FREE: +00800.4573.8000
FAX: +49.6221.4503.45
EMAIL: [email protected]
ASIAN SUPPORT
S. KOREA TOLL FREE: 00798.1.1.002.0297
JAPAN TOLL FREE: (010) 800.40402026
CHINA TOLL FREE: 10.800.711.0752 or 10.800.110.0694
EMAIL: [email protected]
Top View – Shown To Scale
Slide-sized PCR platesSlidetiterTM describes a novel PCR plate which is the footprint of a mi-croscope slide. Not only is the size condensed, but the wall thickness is reduced to half that of conven-tional thin-wall plates for ultra-quick PCR protocols. Four Slideti-ter plates can insert into a single frame producing the equivalent of a standard microplate. This ad-vance allows the use of existing lab equipment to prepare and analyze PCR samples.
Super compact PCR machineThe PikoTM thermal cycler relies upon our Slidetiter plate to achieve a tiny footprint – less than half the size of any cycler. Our unique de-sign delivers unparalleled thermal performance completing a PCR protocol in less than 10 minutes. And with an automated lid, CD drive-like loading mechanism, and multiple block formats (24-, 96- or 384-well) the Piko is a natural fi t for any lab.
Slidetiter frame (blue) with three 96-well Slidetiter plates assembled and one plate removed.
Slidetiter frame (blue) with three 96-
Finnzymes offers new gear for high performance PCR
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* Offer valid for units shipped by December 31st, 2007. See website for more details. Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser’s own internal research and development and applied fi elds other than human in vitro diagnostics under non-real-time thermal cycler patents of Applera Corporation.
