7/29/2019 Actin (C-2)

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SANTA CRUZ BIOTECHNOLOGY, INC.

The Power to Question

Actin (C-2): sc-8432

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

STORAGE

Store at 4 C, **DO NOT FREEZE**. Stable for one year from the date of

shipment. Non-hazardous. No MSDS required.

DATA

SELECT PRODUCT CITATIONS

1. Xiao, G., et al. 2001. Retroviral oncoprotein Tax induces processing of

NF-kappaB2/p100 in T cells: evidence for the involvement of IKKalpha.

EMBO J. 20: 6805-6815.

2. Perskvist, N., et al. 2002. Mycobacterium tuberculosis promotes apopto-

sis in human neutrophils by activating caspase-3 and altering expression

of Bax/Bcl-xL via an oxygen-dependent pathway. J. Immunol. 168:

6358-6365.

3. Castro-Alcaraz, S., et al. 2002. NF-kappa B regulation in human neu-

trophils by nuclear I kappa B alpha: correlation to apoptosis. J. Immunol.

169: 3947-3953.

4. Crone, S.A., et al. 2003. Colonic epithelial expression of ErbB2 isrequired for postnatal maintenance of the enteric nervous system.

Neuron 37: 29-40.

5. Romanelli , RG., et al. 2004. Expression and function of gonadotropin-

releasing hormone (GnRH) receptor in human olfactory GnRH-secreting

neurons: an autocrine GnRH loop underlies neuronal migration. J. Biol.

Chem. 279: 117-126.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

Actin (C-2): sc-8432. Western blot analysis of Actinexpression in C32 whole cell lysate.

Actin (C-2) PE: sc-8432 PE. Intracellular FCM analysisof fixed and permeabilized NIH/3T3 cells. Black linehistogram represents the isotype control, normalmouse IgG1: sc-2866..

< Actin

132 K-

90 K-

55 K-

43 K-

34 K-

BACKGROUND

All eukaryotic cells express actin, which often constitutes as much as 50%

of total cellular protein. Actin filaments can form both stable and labile

structures and are crucial components of microvilli and the contractile appa-

ratus of muscle cells. While lower eukaryotes, such as yeast, have only one

actin gene, higher eukaryotes have several isoforms encoded by a family of

genes. At least six types of actin are present in mammalian tissues and fall

into three classes. Alpha actin expression is limited to various types of mus-

cle, whereas beta and gamma are the principle constituents of filaments in

other tissues. Members of the small GTPase family regulate the organization

of the actin cytoskeleton. Rho controls the assembly of actin stress fibers

and focal adhesion, Rac regulates actin filament accumulation at the plasma

membrane and Cdc42 stimulates formation of filopodia.

REFERENCES

1. Doolittle, R.F. 1995. The origins and evolution of eukaryotic proteins. Phil.

Trans. Royal Soc. London Biol. Sci. 349: 235-240.

2. Maccioni, R.B., et al. 1995. Role of microtubule-associated proteins in the

control of microtubule assembly. Physiol. Rev. 75: 835-864.

SOURCE

Actin (C-2) is a mouse monoclonal antibody epitope mapping at the

C-terminus of human origin.

PRODUCT

Each vial contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide

and 0.1% gelatin.Blocking peptide available for competition studies, sc-8432 P, (100 g

peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).

Available as PE conjugate for flow cytometry, sc-8432 PE, 100 tests.

Available as agarose conjugate for immunoprecipitation, sc-8432 AC,

500 g/0.25 ml agarose in 1 ml.

Available as HRP conjugate for Western blotting, sc-8432 HRP, 200 g/1 ml.

Available as fluorescein (sc-8432 FITC) or rhodamine (sc-8432 TRITC) conju-

gates for immunofluorescence, 200 g/1 ml.

APPLICATIONS

Actin (C-2) is recommended for detection of a broad range of Actin isoforms

of mouse, rat, human and Xenopusorigin by Western Blotting (starting dilu-

tion 1:200, dilution range 1:100-1:1000), immunoprecipitation [12 g per

100500 g of total protein (1 ml of cell lysate)], immunofluorescence and

immunohistochemistry (including paraffin-embedded sections) (starting dilu-

tion 1:50, dilution range 1:50-1:500) and flow cytometry (1 g per 1 x 106

cells).

Suitable for use as control antibody for Actin siRNA (h): sc-29191 and Actin

siRNA (m): sc-29192.

Positive Controls: C32 whole cell lysate: sc-2205, BC3H1 cell lysate:

sc-2299 or Sol8 cell lysate: sc-2249.

Actin (C-2): sc-8432. Immunofluorescence staining ofmethanol-fixed HeLa cells showing cytoskeletallocalization.

Actin (C-2): sc-8432. Immunoperoxidase staining offormalin-fixed, paraffin-embedded human prostatecarcinoma tissue showing cytoskeletal localization.