accelerate your technology deployment · Quantification of peptides and proteins by LC/mS/mS 56 ... This course provides a hands-on introduction to the new features and enhancements

b e a s s u r e d . c h o o s e w a t e r s e d u c a t i o n a l s e r v i c e s .

accelerate your technology deployment

[ COURSE CATALOG 2014 ] DENMARK, FINLAND, IRELAND, NORWAY, SWEDEN, UNITED KINGDOM

NEW Self-Paced e-Learning

Anywhere and at your convenience Do you need training at a convenient time or location?

Do you have an employee who missed on-site training?

Do you need a training refresher?

If you answered yes to any of these questions, then a Waters Self-Paced e-Learning session may be the solution for you.

Self-Paced e-Learning training available in:n HPLC, UPLC, LCMS (Theory, Operation, Maintenance)

n Empower 3, NuGenesis 8, MassLynx, and other Application Managers

n Small and large molecule applications

n Recertification exams

And More…

Benefitsn Learn at your own pace and convenience,

right at your computer

n Save time and eliminate travel costs

n Evaluate your progress with skill assessments

n Reduce learning time by focusing only on the areas you need

n Receive proof of training for your records

...And still get the quality training prepared by Waters trainers

To learn more, go to page 92 or visit

www.waters.com/elearning

*laboratory informatics empower software courses are appropriate for empower, empower 2, and empower 3 software users.

LABORATORY INFORMATICS EmpowEr*what’s New in Empower™ 3 Software? 07Empower™ Software Essentials of the QuickStart Interface 08Empower 3 Fundamentals of the Quickstart Interface 09Empower 3 Quickstart Interface with Lab 10Empower 3 Software Fundamentals of the pro interface 11Empower 3 pro Interface with lab 12Empower 3 Software Advanced Topics 13Empower 3 Advanced Topics: using Administrative Features for productivity 14Empower 3 Advanced Topics: Acquisition, processing and reviewing results 15Empower 3 Advanced Topics: Custom Fields and reports 16Using the waters pDA detectors with Empower3 Software 17Empower 3 method Validation manager 18

LABORATORY INFORMATICS mASSLYNXBiopharmaLynx™ 19ChromaLynx™ XS 20markerLynx™ XS 21massLynx® Data School 22metaboLynx™ 23open Access Login Application manager 24openLynx™ Software 25QuanLynx™ and TargetLynx™ Application manager 26

ULTRAPERFORMANCE LC® (UPLC®) TECHNOLOGYACQUITY UpLC® for Laboratory practitioners 30maintaining the ACQUITY UpLC System 31ACQUITY UpLC Advanced Topics 32ACQUITY UpLC H-Class and ACQUITY UpLC H-Class Bio System for Lab practitioners 33maintaining the ACQUITY UpLC H-Class and ACQUITY UpLC H-Class Bio System 34ACQUITY UpLC I-Class for Laboratory practitioners 35maintaining ACQUITY UpLC I-Class 36ACQUITY UpC2™ for Laboratory practitioners 37maintaining ACQUITY UpC2 38Using ACQUITY QDa™ with Empower 39Using ACQUITY QDa with massLynx 40

OPERATION AND MAINTENANCEAlliance® 2695 operation and Diagnostics 44Alliance 2695/2795 operation and maintenance 45

One stop shopping for all your training needs.

Logon to our training site for:n Training courses, descriptions, and fees

n Latest course schedules

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n And much more

Everything you need – from one single source.

Visit www.waters.com/courses and bookmark it today.

[ TABLE OF CONTENTS ]

MASS SPECTROMETRYCharacterization of peptides and proteins by LC/mS/mS 48Fundamentals of LC/mS 49Interpretation of mS and mS/mS Spectra 50mALDI SYNApT™ G2-S HDmS 51mALDI Imaging operation 52metabolite Identification using LC/mS/mS 53nanoACQUITY UpLC® Techniques and operation 55Quantification of peptides and proteins by LC/mS/mS 56SQD 2 operation and maintenance 57SYNApT® G2-S (Biopharmaceutical) 58SYNApT G2-S (NanoFlow™ proteomics) 59SYNApT G2-S (Small molecules) 60Xevo G2-S Tof / Q-Tof (Biopharmaceutical) 61Xevo G2-S Tof / Q-Tof (NanoFlow proteomics) 62Xevo G2-S Tof / Q-Tof (Small molecules) 63Xevo G2-S Tof / Q-Tof UNIFI™ (Biopharmaceutical) 64Xevo G2-S Tof / Q-Tof UNIFI (Small molecules) 65Xevo TQD operation and maintenance 66Xevo TQ-S operation and maintenance (NanoFlow proteomics) 67Xevo TQ-S (Small molecules) 68Xevo TQ-S UNIFI (Bioanalysis) 69

GENERAL CHROMATOGRAPHYFundamentals of HpLC 72Fundamentals of HpLC (Theory only) 73HpLC method Development with Lab 74HpLC method Development (Theory only) 75Troubleshooting Common HpLC problems 76practical LC method Development with Lab 77

EUROPEAN COURSES 80

ON-SITE EDUCATION 84

eLEARNING 86

REGISTER TODAY 88

[ LABORATORY INFORMATICS ]

[ TABLE OF CONTENTS ]

Empower Driven Services

Advancing Your BusinessEmpower Driven Services integrates instrument services and laboratory intelligence analytics, enabling organizations like yours to optimize their resources through continuous improvements.

With Empower Driven Services, you will know if your resources are being deployed effectively to meet your business needs. n Uncover new areas of cost savings

n Maximize the efficiency of existing assets

n Optimize workflow processes

n Justify new technology adoption

n Integrate with Lean 6 Sigma Initiatives

For more information, go to

www.waters.com/empowerdriven

What’s New in Empower 3 Software?

Language: EnglishLocation: United Kingdom

ref.: 750000567 – 1 day

Course DescriptionThis course shows how to use many features of Empower Chromatography Software. The emphasis is on organizing, acquiring, processing, and reporting chromatography data. This instructor led hands-on computer course is for experienced Empower Chromatography Software users.

Topics Covered ❱ on-line registration for License Activation ❱ Empower 3 Software Help and Search Information ❱ eCord™ data in Empower Configuration manager ❱ process only Sample Sets ❱ ApexTrack™ Changes ❱ Third party Instrument Control Additions and Changes ❱ Empower 3 Software Help resources ❱ Configuration manager Changes ❱ User perspective on system policies and privileges ❱ User accounts, user types, user groups, group administrator ❱ mass Spec enhancements ❱ organizing and accessing information in Empower ❱ Developing a processing method manually in review ❱ ApexTrack Integration ❱ System Suitability enhancements ❱ Altering samples and batch/background processing ❱ reviewing results and calibration curves ❱ Creating reports – Individual and Summary report methods ❱ Customizing headers and footers with logos and special information ❱ Using Empower Software to generate reports for methods

Who Should AttendThis course provides a hands-on introduction to the new features and enhancements offered in Empower 3 chromatography software. The topics are for experienced users who want to access the flexibility built into Empower and their use of the software to meet individual lab application needs. If you are an experienced Empower user, this instructor led training course combined with hands-on computer use is the right course for you.

PrerequisitesAttendees must have at least one year experience using Empower Software. Familiarity with the pro interface is helpful.

Course NotesEach attendee receives a course manual for reference and will receive a Certificate of Attendance. This course uses Empower software and the pro interface.

7For further information and registration, visit www.waters.com/courses


Empower Software Essentials of the QuickStart Interface

Language: EnglishLocation: Ireland

ref.: 750000367 – 1 day

Course DescriptionThis 1-day course shows how to efficiently collect, integrate, calibrate, quantitate and report chromatography data using Empower Chromatography Software via the QuickStart interface. The attendee uses the software throughout the entire course. The instructor led segments and the practice exercises reinforce the skills needed for success.

Topics Covered ❱ Utilisation of the QuickStart interface for acquisition, processing and using existing reports. ❱ Create instrument methods, method sets and sample set methods to run samples ❱ Develop processing methods ❱ Alter sample information ❱ Batch/background processing ❱ Find information ❱ review results and calibration curves ❱ modify existing report methods ❱ Summary exercise (optional)

Who Should AttendThis course is for new users of Empower Software who want to rapidly learn to use the QuickStart interface

PrerequisitesNone.

Course NotesEach attendee receives a course manual for reference during and following the course and will receive a Certificate of Attendance. This course uses only Empower software and the QuickStart interface.

Empower 3 Fundamentals of the Quickstart Interface

Language: Danish, English, Finnish, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden and United Kingdom

ref.: 750000634 – 1 day

Course DescriptionThe Empower Software Fundamentals of the QuickStart Interface course demonstrates how to efficiently collect chromatography data and generate results using Empower Software with the QuickStart interface.

Topics Covered: ❱ overview and login •Empower terminology •overview of the QuickStart interface •Creating an instrument method and method set •Collecting data •Creating a simple sample set •running a sample set •Creating a sample set method •Developing a processing method •overview of the review window •Creating a processing method using the wizard •Understanding and optimizing integration parameters •The component table •Adding a processing method to the method set

❱ Finding information •Using view filters •Sorting data in columns •Using the “view as” function

❱ Alter sample, batch processing and reviewing results •Changing sample information •Batch processing a sample set

❱ reviewing results •manually adjusting integration •manually identifying peaks •reviewing the calibration curve

❱ reporting •Using and modifying a default report method •Adding a report method to the method set

❱ managing projects, systems and user accounts •Creating a project •Backup and restore a project •Copy data •Change project/system •manage the user account

Who Should AttendThis course is for new users of Empower software who want to rapidly learn to use the QuickStart interface.

PrerequisitesNone.

Course NotesEach attendee receives a course manual for reference and will receive a Certificate of Attendance.

98 For further information and registration, visit www.waters.com/courses

[ LABORATORY INFORMATICS ] [ LABORATORY INFORMATICS ]

Empower 3 Quickstart Interface with Lab

Language: English,Location: United Kingdom

ref.: 750000580 (Empower 3) – 2 days

Course DescriptionThis 2-day course shows how to efficiently collect, integrate, calibrate, quantitate and report chromatography data using Empower chromatography software via the QuickStart interface. The attendee uses the software throughout the entire course. The instructor led segments and the practice exercises reinforce the skills needed for success.

Topics CoveredUtilisation of the QuickStart interface for acquisition, processing and using existing reports.

❱ Create instrument methods, method sets and sample set methods to run samples ❱ Develop processing methods ❱ Alter sample information ❱ Batch/background processing ❱ Find information ❱ review results and calibration curves ❱ modify existing report methods ❱ Summary exercise (optional)

Who Should AttendThis course is for new users of Empower software who want to rapidly learn to use the QuickStart interface.

PrerequisitesNone.

Course NotesEach attendee receives a course manual for reference during and following the course and will receive a Certificate of Attendance. This course uses only Empower software and the QuickStart interface.

Empower 3 Software Fundamentals using the Pro Interface

Language: Danish, English, Finnish, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden

ref.: 750000635 – 1 day

Course DescriptionThis 1-day course shows how to efficiently collect, integrate, calibrate, quantitate and report chromatography data using Empower Chromatography Software via the pro interface. The attendee uses the software throughout the entire course. The instructor led segments and the practice exercises reinforce the skills needed for success.

Topics Covered ❱ Utilisation of the pro interface for acquisition, processing and using existing reports. ❱ Create instrument methods, method sets and sample set methods to run samples ❱ Develop processing methods ❱ Alter sample information ❱ Batch/background processing ❱ Find information ❱ review results and calibration curves ❱ modify existing report methods ❱ Summary exercise (optional)

Who Should AttendThis course is for new users of Empower Software who want to rapidly learn to use the pro interface.

PrerequisitesNone.

Course NotesEach attendee receives a course manual for reference during and following the course and will receive a Certificate of Attendance. This course uses only Empower software and the pro interface.



Empower 3 Pro Interface with Lab


ref.: 750000651 – 3 days

Course DescriptionThe learning objective of this 3-day course is to teach the user how to efficiently acquire, process and report chromatographic data using Empower. This instructor led hands-on computer course guides the attendee from log in to running samples through reporting. The course format includes exercises to reinforce the topics covered. practical aspects are treated in the laboratory: they illustrate the theory and allow the use of hardware and software.

Topics CoveredUtilisation of the pro interface for acquisition, processing, using and modifying reports.

❱ Empower overview and Log in ❱ Creating projects ❱ Creating Sample Set methods, Instrument methods and method Sets to run Samples ❱ Developing a processing method to Integrate, Calibrate and Quantitate ❱ modifying Sample Information after Data Acquisition ❱ Batch/Background processing of multiple Chromatographic Data Files ❱ Viewing and Sorting Information in the project window ❱ reviewing results and Troubleshooting Calibration Curves ❱ Generating Individual report methods ❱ Adding Statistical Calculations for peaks ❱ overlaying Chromatograms ❱ Associating Tables with Chromatograms: making Composite report Groups

Who Should AttendThis course is for anyone who wants to build a strong foundation to use Empower. If your answer is no to the following questions, this course is a good choice for you.•Do you know the difference between a sample set and a sample set method?•Can you develop a processing method then confidently batch process data to generate calibration curves and quantify unknowns? •Do you know how to generate printed reports which include document instrument settings and processing parameters?

PrerequisitesThe attendee must review the Empower Getting Started Guide before attending this class.

Course NotesEach attendee receives a course manual for reference during and following the course and will receive a Certificate of Attendance.

Empower 3 Software Advanced Topics

Language: EnglishLocation: Ireland, United Kingdom

ref.: 750000569 (Empower 3) – 3 days

Course DescriptionThis intensive 3-day course shows how to use many advanced features of Empower Chromatography Software. The emphasis is on organising, acquiring, processing, and reporting chromatographic data. Additional topics include adding custom sample descriptions and creating custom calculations for chromatographic data. This instructor led hands-on computer course is for experienced Empower and millennium®

32 Chromatography Software users.

Topics CoveredThe pro interface for organising information, acquiring and processing data, and creating custom fields and reports

❱ Daily Events ❱ Login window properties ❱ The user perspective on system policies and privileges ❱ Understanding the effects of system policies ❱ Default strings ❱ User accounts, user types, user groups, group

administrator ❱ Acquisition servers and chromatographic systems ❱ Cloning projects and multi-project searching ❱ organising and accessing information in Empower ❱ Creating view filters ❱ Using just-in-time view filters ❱ Comparing method versions ❱ Lock/unlock channels ❱ Creating a sample set method template ❱ Developing a processing method manually in review ❱ Traditional vs. ApexTrack detection and integration theory ❱ Global peak detection parameters ❱ Using integration events ❱ Noise and drift calculations in Empower ❱ System Suitability ❱ Altering sample information and batch/background

processing ❱ reviewing results and calibration curves ❱ Adding points, ignoring points ❱ Saving calibration curves, using an earlier calibration

curve, recalculating results ❱ Troubleshooting calibration curves

❱ Creating Custom Fields ❱ Adding sample identifiers using the keyboard or

selection from a list you create for data entry ❱ Adding component specific numerical information ❱ Calculations with peaks ❱ Boolean calculations with peaks ❱ Using text strings as the output for peak calculations ❱ Inter-sample calculations ❱ Creating custom reports- Individual and Summary report

methods ❱ Customizing headers and footers with logos and special

information ❱ Using Empower to generate reports for methods ❱ Using data filters ❱ Creating composite groups ❱ Summarizing statistical information for replicate injects

of one standard ❱ Using overlay chromatograms and ordering tables ❱ Summarizing statistical information for replicate

injections of multiple standards ❱ Using composite groups to associate and order

chromatograms and tables ❱ Summarizing component information for all results in

a result set ❱ Arranging chromatograms and tables next to each other

on the report page

Who Should AttendThis course is for users who know Empower Software software fundamentals and seek fast-paced, intensive training on advanced features using the pro interface. The topics are for experienced users who want to access the flexibility built into Empower and customise their use of the software to meet individual lab application needs. If you are an experienced Empower user, this instructor led training course combined with hands-on computer use is the right course for you.

PrerequisitesAttendees must have at least four months experience using Empower Software. Attending the Empower Software Essentials of the QuickStart Interface course is strongly recommended. Familiarity with the pro Interface is helpful.

Course NotesEach attendee receives a course manual for reference and will receive a Certificate of Attendance. This course uses Empower software and the pro interface.



Empower 3 Software Advanced Topics: using Administrative Features for Productivity

Language: Danish, EnglishLocation: Denmark, Finland, Ireland, Norway, Sweden

ref.: 750000570 (Empower 3) – 1 day

Course DescriptionThis course shows some advanced features of Empower Software. Emphasis is placed on features that can increase productivity for the advanced user such as creating projects, creating and modifying chromatographic systems, user privileges, creating view filters. This instructor led hands-on computer course is for experienced Empower Software users.

Course Topics ❱ Log-in window properties ❱ System policies and privileges ❱ Default strings ❱ User accounts, user types, user groups, group administrator ❱ Acquisition servers and chromatographic systems ❱ Cloning projects and multi-project searching ❱ organizing and accessing information in Empower ❱ Creating view filters ❱ Using just-in-time view filters ❱ Comparing method versions ❱ Lock/unlock channels

Who Should AttendIndividuals who know Empower Software fundamentals and seek fast-paced, intensive training on advanced features using the pro interface. The topics are for experienced users who want to access the flexibility built into Empower and customize their use of the software to meet individual lab application needs. This course uses Empower software and the pro interface.

PrerequisitesAttendees must have at least 3 months experience using the Empower Software pro interface.

Course NotesEach attendee will receive a course manual to keep for future reference and a Certificate of Attendance.

Empower Software Advanced Topics: Acquisition, Processing and Reviewing Results

Language: Danish, English, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden

ref.: 750000571 (Empower 3) – 1 day

Course DescriptionThis course shows how to use advanced acquisition and processing features of Empower software. The class uses both traditional and ApexTrack integration algorithms. This instructor led hands-on computer course is for experienced Empower software users.

Course Topics ❱ Creating a sample set method template ❱ Developing a processing method manually in review ❱ Traditional versus ApexTrack integration ❱ Using Integration Events ❱ Named and timed groups ❱ Baseline subtractions ❱ Calibration and quantitation ❱ Altering samples and batch/background processing ❱ reviewing results and calibration curves

Who Should AttendIndividuals who know Empower Software fundamentals and seek fast-paced, intensive training on the acquisition, processing and reviewing data features using the pro interface.

PrerequisitesAttendees must have at least 3 months experience using Empower Software.




Empower 3 Software Advanced Topics: Custom Fields and Reports

Language: Danish, English Location: Denmark, Finland, Ireland, Norway, Sweden

ref.: 750000573 (Empower 3) – 1 day

Course DescriptionThis course shows you how to use the custom fields and report publisher features of Empower software. Emphasis is placed on adding custom sample descriptions, creating custom calculations and reporting chromatography data and instrument parameters.

Course Topics ❱ Creating Custom Fields ❱ Adding Sample Identifiers Using the Keyboard ❱ Using enumerated data type ❱ Calculations with peaks ❱ Boolean Calculations with peaks ❱ Using Text Strings as the output for peak Calculations ❱ Inter-Sample Calculations ❱ Creating Custom reports—Individual and Summary report methods ❱ Customizing Headers and Footers with Logos and Special Information ❱ Using Empower to Generate reports for methods ❱ Creating Composite Groups ❱ Summarizing Statistical Information for replicate Injections ❱ Summarizing Component Information for All results in a result Set

Who Should AttendIndividuals who know Empower Software Software fundamentals and seek fast-paced, intensive training on the custom fields and report publisher features using the pro interface.

PrerequisitesAttendees must have at least 3 months experience using Empower Software.


Using the Photodiode Array (PDA) Detector with Empower Software

Language: Danish, English, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden, United Kingdom

ref.: 750000577 (Empower 3) – 1 day

Course Descriptionpractical Use of the waters 2996 pDA Detector and Empower Software provides the fundamental information that you need to acquire and utilize pDA data with confidence using Empower Chromatography Software. You will use Empower software with pDA data sets throughout the entire course. The instructor led segments and the practice exercises reinforce the skills needed for utilizing pDA detection for problem-solving.

Topics Covered ❱ pDA overview ❱ Guidelines for collecting 3-dimensional data with the pDA detector ❱ reviewing pDA data ❱ Building a processing method ❱ peak purity ❱ Creating a UV Library and Library matching ❱ reporting pDA Data

Who Should AttendEmpower Software users who want to rapidly learn to utilize the capabilities of the pDA detector for 3D analysis.

PrerequisitesThe class attendee must be familiar with either the Empower QuickStart or pro Interface. Knowing how to create a processing method with 2-dimensional chromatography data is helpful.




Empower Software: Method Validation Manager (MVM)

Language: Danish, EnglishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000578 (Empower 3) – 3 days

Course DescriptionThis 3-day course is designed to walk you through all aspects of the method Validation manager option in Empower. It is designed to logically follow the steps in the method Validation workflow process from creating a validation method protocol to reporting and signing off validation results. This instructor led hands-on computer course guides the attendee through the topics.

Topics Covered ❱ overview of method Validation ❱ Administering method Validation Activities ❱ manager and Validation review ❱ Validation protocol methods ❱ Configuring Validation Tests (i.e., linearity, repeatability, accuracy, etc) ❱ workflow of a Validation Study ❱ Creating and Viewing Validation results ❱ reporting Data and Signing off method Validation results ❱ method Validation Troubleshooting

Who Should AttendEmpower software users or managers who want to use, set-up or administer the method Validation manager to automate the entire chromatographic method validation process.

PrerequisitesmVm only works with the Empower pro Interface. Therefore, attendees must be familiar with Empower pro Interface and know how to acquire process and print chromatographic data. A basic understanding of method validation theory is assumed.


BiopharmaLynx

Language: EnglishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000474 – 1 day

Course Description A 1-day training course designed for BiopharmaLynx users to cover optimised data acquisition, processing and interpretation. During this training course there will be opportunity to work with other attendees and waters application experts on this massLynx application manager.

Topics Covered ❱ A review of experiment design ❱ peptide mapping and intact analysis ❱ BiopharmaLynx processing and method creation ❱ result evaluation and interpretation ❱ Validation of software assignments using mSE

Daily EventsThe major focus will be hands on data processing with defined exercises to reinforce the skills taught.

Who Should AttendmS and massLynx users who would like to utilise functionality offered by BiopharmaLynx application manager.

PrerequisitesAttendance of a basic operator training course for ToF mass Spectrometer. Usage of the current software revision of massLynx with BiopharmaLynx application manager.




ChromaLynx and ChromaLynx XS

Language: English, SwedishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000536 – 2 days

Course Description A 2-day training course designed for ChromaLynx users to cover optimised data acquisition, processing and interpretation. During this training course there will be opportunity to work with other attendees and waters application experts on this massLynx application manager. The ChromaLynx software package reliably and automatically detects chromatographic peaks. It is essential to perform deconvolution in complex mixture analysis in order to fully profile the extract. It is able to accurately produce pure spectra from unresolved closely eluting peaks. Exact mass measurement aids in the confirmation of identity of components in a complex matrix. ChromaLynx software works with both high and low resolution LC/mS, GC/mS, LC/mS/mS (Q-Tof) and GC/mS/mS (Q-Tof) data.

Topics CoveredThis training course is designed to teach the operator:

❱ The ChromaLynx XS work-flow ❱ ChromaLynx Identify – profiling of complete sample. ❱ ChromaLynx Targeted – targeted screening and confirmation. ❱ ChromaLynx Compare – comparison of complex samples for chromatographic differences and similarities. ❱ poSI±IVE – Automated identification and quantification of targeted analytes with spectral confirmation. ❱ Generating reports.

Default data sets and exercises will be provided on this largely practical training course. Customers are encouraged to bring data sets and problems to the workshop.

Who Should AttendmS and massLynx users who would like to utilise functionality offered by ChromaLynx XS application manager.

PrerequisitesAttendance of a basic operator training course for mass Spectrometer. Usage of the current software revision of massLynx with ChromaLynx application manager.


MarkerLynx XS

Language: EnglishLocation: Finland, Germany, Norway, United Kingdom

ref.: 750000473 – 2 days

Course Description A 2-day training course designed for markerLynx users to cover optimised data acquisition, data processing and interpretation. That also offers the opportunity to work with other attendees and waters application experts on the massLynx application manager.

Topics Covered ❱ A review of experiment design ❱ peak detection and data filtering ❱ Advanced data analysis using eXtended Statistics ❱ Database searching ❱ Daily Events ❱ Defined Data exercises to reinforce the skills taught.

Who Should AttendmS and massLynx users who would like to utilise functionality offered by markerLynx XS application manager.

PrerequisitesAttendance of appropriate mS system operator training course. Usage of the current software revision of massLynx with markerLynx application manager.




MassLynx Data School


ref.: 7560000356 – 1 day

Course Description This course covers the basic operation of the massLynx software package as used on LC/mS and LC/mS/mS system. Hands-on massLynx software training enhances and reinforces lecture material. Upon completion of this course, the attendee should be able to confidently use the massLynx software.

Topics Covered ❱ Introduction to massLynx version 4.1 ❱ massLynx Architecture ❱ Tuning and Calibrating ❱ Data Acquisition ❱ Data processing, reporting Data ❱ Quantitation and reporting ❱ massLynx other tools ❱ Introduction to openLynx

Who Should AttendThis course is for users who have no experience or limited skills with massLynx version 4.1 software. This course is also for infrequent users of massLynx version 3.5 who will now use massLynx version 4.1. Due to their presence in massLynx version 4.1, an introduction to QuanLynx and openLynx application managers will also be covered.

PrerequisitesNone.


MetaboLynx XS


ref.: 750000358 – 2 days

Course Description A 2-day training course designed for metaboLynx users to cover optimised data acquisition, processing and interpretation. During this training course there will be opportunity to discover or improve knowledge in metaboLynx features and work with other attendees and waters application experts on this massLynx application manager.


❱ Data acquisition ❱ The dealkylation tool ❱ mass defect filtering ❱ metaboLynx XS browser ❱ mSE data processing ❱ massFragment™ ❱ Automating mSmS run lists


Who Should AttendmS and massLynx users who would like to utilise functionality offered by metaboLynx XS application manager.

PrerequisitesAttendance of appropriate mS system operator training course. Usage of the current software revision of massLynx with metaboLynx application manager.




Open Access login Application Manager

Language: EnglishLocation: Denmark, Finland, Norway, United Kingdom

ref.: 750000359 – 2 days

Course Description This course covers the operation of the open Access Login software package as used on LC/mS and LC/mS/mS system. Hands-on open Access Login Software training enhances and reinforces lecture material. Upon completion of this course, the attendee should be able to confidently use the open access Login software.


❱ open Access Login Software Installation ❱ open Access Software overview ❱ wizard configuration ❱ Instrument management ❱ Samples management ❱ Computer management ❱ Computer maintenance ❱ Network protocol ❱ Troubleshooting ❱ oA Toolkit software overview ❱ remote status monitoring

Who Should AttendThis course is dedicated to LC/mS or LC/mS/mS System administrators; people who are responsible for the equipment and who have limited skills or low experience with open Access login Sofware. A network part is added to improve computer configuration, software installation and understanding. If you are an inexperienced massLynx user, this instructor led training course combined with hands-on computer use is the right course for you.

PrerequisitesAttendees must have at least few weeks experience using open Access Login application manager Software. A good working knowledge of the massLynx software is required.


OpenLynx Software

Language: EnglishLocation: Denmark, Finland, Norway, United Kingdom

ref.: 750000153 – 1 day

Course Description This course covers the operation of the openLynx Software package as used on LC/mS and LC/mS/mS sytem. Hands-on openLynx software training enhances and reinforces lecture material. Upon completion of this course, the attendee should be able to confidently use the openLynx software.

Topics Covered ❱ mS reminder ❱ massLynx reminder ❱ openLynx software overview ❱ openLynx set-up ❱ openLynx method building ❱ Data processing ❱ openLynx browser ❱ report method building ❱ Data review ❱ openLynx manager ❱ Data sending configuration ❱ Data printing


Who Should AttendThis course id dedicated to LC/mS or LC/mS/mS system administrators; people who are responsible for the equipment and who have limited skill level or low experience with openLynx software. If you are an experienced massLynx user, this instructor led training course combined with hands-on computer use is the right course for you.

PrerequisitesAttendees must have at least few weeks experience using openLynx application manager software. A good working knowledge of the massLynx software is also required.




QuanLynx and TargetLynx Application Manager

Language: English, SwedishLocation: Finland, Norway, Sweden, United Kingdom

ref.: 750000357 – 1 day

Course Description This course covers the basic operation of the QuanLynx and TargetLynx software package as used on LC/mS and LC/mS/mS system.


❱ Quantification process overview ❱ Using external, internal Standard ❱ QuanLynx Software overview ❱ Sotware architecture, processing method building ❱ Data processing, data review ❱ Quanoptimize™ ❱ Software overview and method building ❱ Introduction to TargetLynx ❱ TargetLynx software overview ❱ Software Architecture, processing method building ❱ Data processing


Who Should AttendThis course is for users who have no experience or limited skills with QuanLynx and TargetLynx software. If you are an experienced massLynx user, this instructor led training course combined with hands-on computer use is the right course for you.

PrerequisitesAttendees must have 6-8 weeks experience using QuanLynx and TargetLynx application manager Software. A good working knowledge of the massLynx software is required.


26


[ ULTRAPERFORMANCE LC (UPLC) TECHNOLOGY ]

Waters Educational Services: Fast Facts

n 6,000 users trained each year

n 6 corporate training centers across the globe

n More than110 training locations in 30 countries

n Specialized and certified instructors

n More than 95 courses covering theory, practice, maintenance, and qualification:n LC, MS, UPLC small and large applicationsn Informaticsn Waters Instruments and Softwaren General Chromatography and LC/MS fundamentals n Compliancen SFC

n Flexible delivery options: class room, on-site, and online

n Most courses available at your location

Special Discounts for Warranty and Support Plan Customers

If your HPLC, UPLC , and mass spectrometry systems are under a support warranty or plan, you can receive up to 20% discount on any instrument, software, or application course. Please contact your local Waters training coordinators for more information.

ACQUITY UPLC for Laboratory Practitioners

Language: Danish, English, SwedishLocation: Denmark, Ireland, Finland,Norway, Sweden, United Kingdom

ref.: 750000327 – 2 days

Course Description This course explains how the ACQUITY UpLC system combines new chemistry with advances in instrumentation to access increased speed, resolution and sensitivity in chromatographic analysis. Included in the course are exercises such as becoming familiar with the ACQUITY Console software, priming the instrument, running test and sample solutions, and method transfer.

Topics Covered ❱ Understanding the unique differences between HpLC and UpLC with•An introduction and global overview•An in-depth look at UpLC theory and chemistry•An overview of the ACQUITY UpLC System’s hardware and configuration

❱ Using the ACQUITY Console software for:•priming the Binary Solvent manager and Sample manager•Calibrating the Sample manager•Setting default parameters•General troubleshooting techniques, including tips and tricks

❱ method transfer:•Theory behind transfer of method from HpLC to UpLC.•Using the ACQUITY UpLC column calculator

Who Should AttendThis course is for individuals who want to push the limits of conventional HpLC and understand the chemistry, operating principles of the ACQUITY Ultraperformance LC® system. Ultraperformance LC presents the possibility to extend and expand the utility of this widely used separation science.

PrerequisitesAttendees should be familiar with the basic principles of HpLC.


Maintaining the ACQUITY UPLC System

Language: Danish, English, Finnish and, SwedishLocation: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000328 – 1 day

Course Description This course explains how to perform routine maintenance for optimal performance. Included in this course are exercises that will enable you become familiar with routine and more advanced user maintenance procedures.

Topics Covered ❱ maintaining your ACQUITY UpLC System for optimal performance and reliability by:•replacing components during a routine maintenance such as the pump head seals, plunger, check valves, injection needle and syringes•Having information to change the detector lamp and clean the flow cell•General troubleshooting techniques, including tips and tricks•Utilizing Connections INSIGHT® with its Intelligent Device management (IDm) technology

Who Should AttendThis course is for individuals who are responsible for the day-to-day user maintenance of their ACQUITY Ultraperformance LC system.

PrerequisitesAttendees should be familiar with the basic principles of HpLC, and preferably have followed the “ACQUITY UpLC for Laboratory practitioners” course.



[ UltraPerformance lc ] [ UltraPerformance lc ]

ACQUITY UPLC Advanced Topics


ref.: 750000451 – 1 day

Course Description The aim of this training course is to update the knowledge of the ACQUITY UpLC user with the latest developments in ACQUITY UpLC Technology and working practices. Also an opportunity to evaluate your own knowlege of this important technology will be offered.

Topics Covered ❱ review of Latest Feature Developments •Hardware•Software•Chemistry

❱ Introduction to UpLC method Development•mobile phase selection•Column selection

❱ HpLC to UpLC method Transfer review •Considerations•main rules to follow

❱ Tips and Tricks•major contaminants and their sources•Controlling and Troubleshooting contamination•Cleaning to eliminate contamination

Who Should AttendThis course is for individuals who are responsible for the operation of their laboratory’s ACQUITY Ultraperformance LC system and would like to update themselves with the latest features and practices.

PrerequisitesA working knowledge of ACQUITY UpLC. Attendance of a waters UpLC training course, e.g. ACQUITY UpLC for Lab practitioners would be advantageous.


ACQUITY UPLC H-Class for Laboratory Practitioners and ACQUITY UPLC H-Class Bio

Language: Danish, English, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden, United Kingdom

ref.: 750000523 – 2 days (H-Class)ref.: 750000551 – 2 days (H-Class Bio)

Course Description Instruction for the ACQUITY UpLC H-Class is a two day course designed to offer a solid understanding of ACQUITY UpLC H-Class Chemistry, Instrumentation and Software. Instrument overview, chromatographic concepts as well as method Transfer and Development will be taught.

Topics Covered ❱ ACQUITY UpLC H-Class and H-Class Bio Introduction and overview ❱ HpLC/UpLC Chemistry ❱ Using the Console•Starting the System•priming the pump and Fluidic pathway•Diagnostics

❱ Quaternary Solvent manager ❱ Isocratic and Gradient Solvents ❱ Sample manager•Injections mechanism using Flow Through Needle (FTN) design

❱ System Configuration ❱ Detection mechanisms ❱ method Transfer and Development ❱ Connections INSIGHT ❱ practical Lab Exercises•Determine System Dwell Volume•System Setup running Test Solutions•Using the ACQUITY UpLC Calculator•Using the Column Selectivity Chart

Who Should AttendThe course is designed for attendees who currently use the ACQUITY UpLC H-Class System. For the user who is involved in Instrument operation and for those whose responsibilities include method Transfer and/or method Development.

PrerequisitesAttendees should have the ACQUITY UpLC H-Class Systems installed in their lab. A working knowledge of Empower 2 or massLynx is strongly recommended.




Maintaining the ACQUITY UPLC H-Class Practitioners and ACQUITY UPLC H-Class Bio

Language: Danish, English, Finnish, SwedishLocation: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000524 – 1 day (H-Class)ref.: 750000552 – 1 day (H-Class Bio)

Course Description This course covers basic troubleshooting and routine maintenance on the ACQUITY UpLC H-Class and H-Class Bio System. participants will learn how to carry out performance maintenance (pm) on an ACQUITY UpLC H-Class System and perform the built in diagnostics to verify the repair/maintenance to ensure the instrument is at its highest performance level.

Topics Coveredmaintaining your ACQUITY UpLC H-Class System for optimal performance and reliability by•ACQUITY System overview •ACQUITY console software •Changing pump seals, pistons & check valves•Changing injector needle & syringes •running the static leak test •X, Y, Z axis calibration •ACQUITY diagnostics

Who Should AttendThe course is designed for users who currently use the ACQUITY UpLC H-Class Systems.

PrerequisitesAttendees should be familiar with the basic principles of HpLC, and preferably have followed the ‘‘ACQUITY UpLC for Laboratory practitioners’’ course.


Who Should AttendAll ACQUITY UpLC I-Class system users.



ACQUITY UPLC I-Class for the Lab Practitioner

Language: Danish, English, SwedishLocation: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000607 – 2 days

Course DescriptionThis course explains how the ACQUITY UpLC I-Class system combines new chemistry with advances in instrumentation to access increased speed, resolution and sensitivity in chromatographic analysis.

Topics Covered ❱ UpLC theory and chemistry•UpLC principle and theory •resolution and peak capacity •UpLC column chemistries •Column design •System bandspreading

❱ ACQUITY hardware •Solvent manager •Degasser •Sample manager •Column manager •eCord technology •Detectors

❱ ACQUITY console software •overview of console software •module functions and diagnostics •Interactive displays •plots and logs •preparing for analysis •Characterizing needle and loop volume •Instrument method parameters

❱ method transfer, HpLC to UpLC •method transfer considerations •Transfer of isocratic methods •Transfer of gradient methods •System delay volume •Using the ACQUITY column calculator

❱ Troubleshooting tips and tricks •mobile phase preparation •Controlling bacterial growth •Column maintenance •Tips and tricks for the solvent and sample manager •optimal detector settings •Troubleshooting peak shape problems and carry over

❱ Hands-on exercises •Column efficiency test •System precision test •Gradient performance test •method transfer exercise



Maintaining ACQUITY UPLC I-Class

Language: Danish, English, Finnish, SwedishLocation: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000608 – 1 day

Course DescriptionThe course covers how to do performance maintenance on the ACQUITY UpLC I-Class system, including changing seals, pistons and injector needle.

Topics Covered ❱ ACQUITY system overview ❱ ACQUITY console software ❱ Changing pump seals, pistons and check valves ❱ Changing injector needle and syringes ❱ running the static leak test ❱ X, Y, Z axis calibration ❱ ACQUITY diagnostics

Who Should AttendIndividuals who needs to perform maintenance on the ACQUITY UpLC system.

PrerequisitesAttendees should be familiar with the basic principles of UpLC.


ACQUITY UPC2 for Lab Practitioners

Language: Danish, English, SwedishLocation: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000623 – 2 days

Course DescriptionThis course explains how the ACQUITY UpC2 System combines new chemistry with advances in instrumentation to access increased speed, resolution and sensitivity in chromatographic analysis. Included in the course are exercises such as basic login for Empower and massLynx, becoming familiar with the ACQUITY Console software, priming the instrument, running test and sample solutions, and method transfer.

Topics Covered ❱ Understanding the unique differences between SFC and UpC2 with an introduction and global overview ❱ A brief refresher of SFC and an in-depth look at UpC2 chemistry ❱ An overview of the ACQUITY UpC2 System’s hardware and configuration ❱ Using the ACQUITY Console software for: •priming the Binary Solvent manager and Sample manager •Calibrating the Sample manager •Setting default parameters

❱ maintaining your ACQUITY UpC2 System for optimal performance and reliability: ❱ method Transfer and optimisation from SFC to UpC2

❱ General troubleshooting techniques, including tips and tricks ❱ Utilizing Connections INSIGHT with its Intelligent Device management (IDm) technology

Who Should AttendThis course is for individuals who want to push the limits of conventional SFC and understand the chemistry, operating principles and user maintenance of the ACQUITY UpC2 System. Ultraperformance SFC™ presents the possibility to extend and expand the utility of this widely used separation science.

PrerequisitesAttendees should be familiar with the basic principles of SFC.




Maintaining ACQUITY UPC2

Language: Danish, English, Finnish, Swedish Location: Denmark, Ireland, Finland, Norway, Sweden, United Kingdom

ref.: 750000624 – 1 day

Course DescriptionThis course explains how to operate and perform routine maintenance for optimal performance. Included in this course are exercises that will enable you to become familiar with routine and more advanced user maintenance procedures.

Topics Coveredmaintaining your ACQUITY UpLC System for optimal performance and reliability by:

❱ replacing components during a routine maintenance such as the pump head seals, plunger, and needle assembly ❱ Having information to change the detector lamp and clean the flow cell ❱ General troubleshooting techniques, including tips and tricks ❱ Utilizing Connections INSIGHT with its Intelligent Device management (IDm) Technology

Who Should AttendThis course is for individuals who are responsible for the day to day maintenance of their Ultraperformance Convergence Chromatography™ (UpC2) System.

PrerequisitesAttendees should be familiar with the basic principles of HpLC, and have followed the “ACQUITY UpC2 for Laboratory practitioners” course.


ACQUITY QDa with Empower

Language: English, French, GermanLocation: To be confirmed

ref.: 750000833 – 2 days

Course DescriptionThis course introduces you to the ACQUITY QDa detector hardware and operating principles in an Empower softwareenvironment. Students are shown ACQUITY QDa detector specific topics within the Empower software, such as instrumentmethod settings and working with mass spectral data in the review window. Lab exercises are performed to practice andreinforce Empower mS data processing concepts needed to become successful with the QDa detector and Empower software.

Topics Covered ❱ ACQUITY QDa Detector principles and hardware ❱ preparing to run•ACQUITYQDaDetectorConsole•ACQUITYQDaDetectorinstrumentmethodsettings

❱ Empower review 3D window overview•Toolbarfunctions•Tableproperties•Extractingchromatograms•Extractingspectra•Spectrumplotproperties•3Dview•Developingaprocessingmethodfor3Ddata

❱ mass analysis window (for Empower 3 Fr2)•Extractingachannel•MSspectraextraction•Massanalysiswindowtoolbar•Massanalysiswindowviewproperties

❱ User maintenance•Sourcepartreplacement/cleaning•Capillaryreplacement

Who Should AttendEmpower software users who want to rapidly learn the capabilities of the ACQUITY QDa detector.

PrerequisitesAttendee must be familiar with Empower Software using the pro Interface. Knowing how to work with 2-dimensionalchromatography data in Empower Software as well as an understanding of foundational LC/mS principles is helpful.




ACQUITY QDa with MassLynx

Language: English, French, GermanLocation: To be confirmed

ref.: 750000834 – 2 days

Course DescriptionThis course introduces you to the ACQUITY QDa detector hardware and operating principles in a massLynx softwareenvironment. Students are shown ACQUITY QDa detector specific topics within the massLynx software, such as instrumentmethod settings and working with mass spectral data in the chromatogram and spectrum windows. Lab exercises areperformed to practice and reinforce data processing concepts needed to become successful with the QDa detector andmassLynx software.

Topics Covered ❱ ACQUITY QDa Detector principles and hardware ❱ preparing to run•ACQUITYQDaDetectorConsole•ACQUITYQDaDetectorinstrumentmethodsettings

❱ massLynx chromatogram window•Displayingchromatograms•Changingthechromatogramdisplay•Processingchromatograms

❱ massLynx spectrum window•Displayingspectra•Processingspectra

❱ openLynx application manager ❱ User maintenance•Sourcepartreplacement/cleaning•Capillaryreplacement

Who Should AttendmassLynx software users who want to rapidly learn the capabilities of the ACQUITY QDa detector.

PrerequisitesAttendee must be familiar with data acquisition using massLynx software. An understanding of foundational LC/mSprinciples is helpful.




Waters Software Deployment Services

Reduce the time and cost of your software deploymentW hether you are upgrading your software system or installing software for the first time, Waters consultants have the skills and expertise to make your implementation a success.

Available deployment services include:n Project Management

n Architecture and Infrastructure Consultation

n Installation/Configuration Assistance

n Application Assessment/Audit Services

n Software Validation

n Instrument/Software Qualification

n Training Services

n Integration Services

To learn more, go to

www.waters.com/deployment

[ OPERATION AND MAINTENANCE ]

http://www.waters.com/deployment

Alliance 2695 Operation and Diagnostics


ref.: 750000427 – 1 day

Course Description The aim of the course is to give the participants a thorough understanding of the operating principles in the 2695 separations module. After completion of the course the participants should be able to identify and diagnose instrumentation issues to keep their Alliance HpLC system running at the highest level of performance.

Topics Covered ❱ System overview/ Design of the 2695 ❱ Solvent and sample management technologies ❱ Direct functions and diagnostics ❱ Instrument Setup and troubleshooting procedures ❱ Dry/wet prime ❱ Seal wash and Needle wash ❱ Injector compression test ❱ Screw/Ferrule replacement ❱ Changing check valves, in-line filters and seals

Who Should AttendUsers of the 2690/2695 separations module who want to understand the operation of the 2695 separations module and perform basic troubleshooting on their system.

PrerequisitesBasic knowledge of HpLC.


Alliance 2695/2795 Operation and Maintenance

Language: Danish, English, Finnish, SwedishLocation: Denmark, Finland, Ireland, Norway, Sweden

ref.: wAT038306 – 2 days

Course Description This course instructs users in the operation, maintenance and trouble shooting of waters 2695/2795 Separations module. Emphasis is on solvent and sample management technology. This course includes lectures followed by practical sessions that reinforce the lecture material. After completion of this course, attendees should be able to identify and diagnose instrumentation issues, to keep their Alliance HpLC System running at the highest level of performance.

Topics Covered ❱ Sample and Solvent management Technology ❱ Instrument Configuration ❱ Direct Functions ❱ Creating separation methods ❱ Creating sample sets and templates ❱ Diagnostics ❱ Hands on: Day-to-day maintenance and troubleshooting

Who Should AttendThis course is designed for individuals who are responsible for the routine performance maintenance of waters 2695/2795 Separations module.

PrerequisitesNone.



[ OperatiOn and Maintenance ] [ OperatiOn and Maintenance ]

Waters Educational Services is Just Around the Corner

Waters Educational Services courses are available around the world. Our major training centers are:n Waters World Wide Training Center –

Milford, MA, U.S.A.

n Waters European Headquarters and Training Center – St. Quentin-en-Yvelines, France

n Waters Asian Training Center – Singapore

n Waters World Wide Mass Spectrometry Center of Excellence – Manchester, United Kingdom

n Waters North American Mass Spectrometry Center – Beverly, MA, U.S.A.

n Waters HPLC/Mass Spectrometry Center West Coast – Pleasanton, CA, U.S.A.

We also offer courses at each of our offices, with additional training centers in China, India, Japan, and Puerto Rico. Each year, more than 6,000 students visit our training sites in over 30 countries.

To find out all the locations near your area, go to

www.waters.com/courses and select a country and the courses of your choice. It’s that easy.

[ MASS SPECTROMETRY ]

Characterization of Peptides and Proteins by LC/MS (/MS)

Language: EnglishLocation: Denmark, Sweden and United Kingdom

ref.: 750000467 – 2 days

Course DescriptionThis course is designed for people that need to know how LC/mS (/mS) can be used for characterization of peptides andproteins. The course consists of lectures and exercises.

Topics Covered ❱ Introduction to mS analysis of peptides and proteins ❱ practical aspects of ESI-mS and LC/mS analysis of peptides and proteins ❱ Data processing and information from mass spectra ❱ Instrumentation and strategies for peptide sequencing ❱ Fragmentation of peptides in mS-mS, fundamentals and examples ❱ Interpretation of mS-mS spectra of peptides ❱ Software tools for the interpretation of mS-mS spectra ❱ Strategies and perspectives in peptides and protein characterization and proteomics

Who Should AttendEveryone that wants an introduction to the field of characterization of peptides and proteins by LC/mS.

PrerequisitesBasic knowledge of HpLC and LC/mS.


Fundamentals of LC/MS

Language: Danish, English, SwedishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000167 – 1 day

Course Description “Fundamentals of LC/mS” course provides information to make an effective transition from traditional HpLC detectors to mS detectors. The course covers relevant current ionization techniques and mass analyzers. Consideration is also given to converting existing HpLC methods for use with mS detectors. practical examples guide the user on how to modify the HpLC separation to make them suitable for mS detection.

Topics Covered ❱ mass Spectrometry overview ❱ Atmospheric pressure Ionization Fundamentals ❱ Selecting an appropriate mS ionization technique ❱ overview of mass analyzers ❱ Liquid Chromatography Considerations ❱ Conversion of established HpLC methods for use with an mS detector

Who Should AttendIndividuals with no or little LC/mS knowledge that needs to learn the fundamental principles of LC/mS, or persons responsible for developing new, or transferring existing, HpLC methods for use with an mS detector. Current practitioners of LC/mS may also benefit if their understanding of the technique is limited and they have had no formal training in LC/mS techniques.




[ MASS SPECTROMETRY ] [ MASS SPECTROMETRY ]

Interpretation of MS and MS/MS Spectra

Language: EnglishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000466 – 2 days

Course Description The aim of this course is to study the fragmentation of positively and negatively charged even-electron ions of small molecules during in-source CID or mS/mS in triple-quadrupole, ion-trap, and quadrupole time-of-flight hybrid systems. The emphasis is on spectrum interpretation rather than on fragmentation mechanisms. In addition to lectures and ample time for questions and discussion, the course provides exercises in order to practice spectral interpretation.

Topics Covered ❱ Introduction to mS/mS ❱ Theoretical aspects of collision induced dissociation, CID ❱ Fragmentation techniques ❱ In-source CID ❱ Triple quadrupole ❱ Ion-trap ❱ Quadropole-ToF ❱ General aspects of fragmentation of even-electron positive ions ❱ Fragmentation of different compound classes, illustrated with typical examples ❱ Fragmentation of even-electron negative ions ❱ Strategies in structure elucidation.

Who Should AttendThis course is designed for individuals, who are new users to LC/mS or mS/mS want to learn how to interpret mass spectra, or existing users who want to supplement their skills. This course is for all LC/mS and mS/mS and is not specific for users of waters/micromass mS instruments.

Prerequisites6 months experience with LC/mS or mS/mS.


MALDI SYNAPT G2-S HDMS Operation

Language: English Location: waters European Training Center in manchester (United Kingdom)

ref.: 750000656 – 3 days

Course DescriptionA comprehensive mALDI SYNApT G2-S HDmS operation training course for biopharmaceutical applications. Hands-on massLynx training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters mALDI SYNApT G2-S.

Topics Covered ❱ overview of mALDI ❱ ToF Accurate mass overview•ToF theory•Use of accurate mass data

❱ Instrument optimization, data acquisition•SYNApT G2-S instrument Schematics•Tuning and calibration•mS and mS/mS data acquisition

❱ routine maintenance ❱ massLynx software overview ❱ mALDI sample preparation ❱ HDmS™ – acquisition of data using ion mobility•DriftScope™

Daily EventsDefined lab exercises to reinforce the skills taught.

Who Should Attendpersonnel responsible for operation of mALDI SYNApT G2-S.

PrerequisitesAttendees should have a minimum of 6 weeks experience operating the instrument.

Course Notes Each attendee will receive a course manual to keep for future reference and a Certificate of Attendance.



MALDI Imaging Operation


ref.: : 750000757– 3 days

Course DescriptionA comprehensive waters mALDI SYNApT G2-S operation training course for Imaging applications. Hands-on massLynx, and High Definition Imaging (HDI) imaging software training, including laboratory sessions enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on the mALDI SYNApT G2-S.

Topics Covered ❱ Introduction•overview of mALDI imaging workflows using the mALDI SYNApT G2-S and HDI imaging software

❱ Sample preparation•manual spray coating•SunCollect mALDI-spotter (Sunchrom) spray coating

❱ Instrument tuning for mALDI imaging•Quad profile•Calibration•Ion mobility

❱ Data acquisition•Experimental set-up•Untargeted imaging workflows (mS, ion mobility-mS and mSE acquisitions)•Targeted imaging workflows (mS/mS and ion mobility mS/mS acquisitions)•Lockmass set-up

❱ HDI imaging software •pattern creation•processing•Image analysis


Who Should Attendpersonnel responsible for operation of mALDI SYNApT G2-S.



Metabolite Identification using LC/MS/MS Technology

Language: EnglishLocation: Denmark

ref.: 750000582 – 2 days

Course DescriptionThe two day course in metabolite identification using LC/mS technologies is directed at researchers in the field of metaboliteidentification, mostly involved in early-ADmE and DmpK within early stages of drug discovery and development withinpharmaceutical industries.

Topics Covered ❱ General Introduction. •(Early-)ADmE studies. •General workflow in metabolite profiling and identification. •Considerations on LC and sample pretreatment. •radioactivity detection.

❱ phase I metabolism from biological and chemical perspective. •Consequences in mS analysis: mass shifts. •In vitro and in vivo studies. •reactive metabolites.

❱ Strategies for detection and structure elucidation of metabolites using LC/mS/mS.

1. Phase I metabolism. •mass shifts in complementary fragments and profile groups to identify metabolites. •Exercises.

2. Phase I metabolism. •Data-dependent LC/mS analysis: potentials and limitations. •Scan modes in mS/mS: precursor-ion, neutral-loss analysis modes. •Isotope-cluster techniques. •Fraction collection and off-line mS analysis.•H/D-exchange experiments. •metabolic profiling. •Electrochemistry-mS in metabolite studies. •Exercises.

3. Accurate-mass determination. •potentials and limitations. •practical issues: external and internal calibration. •predicting elemental compositions. •Accurate mass and isotope patterns to derive elemental composition. •Interpretation of mS/mS spectra and elucidating ambiguities. •mSE analysis modes. •mass-defect filtering. •Data-processing strategies. •Exercises.

>>> Course Description continues on next page.



Metabolite Identification using LC/MS/MS Technology

4. Reactive metabolites. •Idiosyncratic toxicity. •reactive metabolites from biological and chemical perspective. •Strategies for detection and structure elucidation of reactive metabolites using LC/mS/mS. •Glutathione conjugates and cyano-trapping. •precursor-ion and neutral-loss analysis modes. •Srm-triggered data-dependent analysis. •Isotope-cluster analysis. •Consequences in drug design. •Exercises.

❱ phase II metabolism from biological and chemical perspective. •Strategies for detection and structure elucidation of phase II metabolites using LC/mS/mS. •mass shifts and typical fragmentation of conjugates. •precursor-ion and neutral-loss analysis modes. •reactive metabolites. •Exercises.

Who Should AttendAnyone working in the field of metabolite identification using LC/mS.

PrerequisitesIt will be an advantage to have participated in the “Interpretation of mS and mS-mS spectra” course.


nanoACQUITY UPLC Techniques and Operation


ref.: 750000470 – 2 days

Course Description This two-day training course is designed for the nanoACQUITY UpLC system users to cover the chemistry, operating principles and maintenance.

Topics Covered ❱ NanoFlow chromatography and benefits of 1.7 µm particles ❱ Introduction to nanoACQUITY UpLC hardware and design features ❱ Full description of nanoACQUITY UpLC flowpath and possible valve configurations ❱ Using the nanoACQUITY UpLC Console software for system startup, including priming the Binary Solvent manager

and Sample manager and calibrating the Sample manager ❱ Column plumbing, leak testing and column equilibration ❱ Setup, acquisition and evaluation of over night test runs ❱ General troubleshooting techniques, including tips and tricks ❱ General Discussion forum and Q&A’s

Who Should AttendThis course is for individuals who wish to understand the chemistry, operating principles and user maintenance of the nanoACQUITY UpLC system.

PrerequisitesAttendees should be familiar with the basic principles of HpLC and mS.




Quantification of Peptides and Proteins by LC/MS/MS

Language: English, SwedishLocation: Sweden

ref.: 750000649 – 2 days

Course DescriptionA two day practical training course covering development of methods for quantification of peptides and proteins with LC/mS/mS.

Topics Covered ❱ proteins and peptides theory:•Characteristics of peptides•Differences from small molecules•Differences between peptides

❱ Strategies for method Development: mS•Triple quad theory•Setting up mrm transitions for quantification•Enzymatic digest for proteins•Selecting peptide to quantify LC•mobile phase selection•pH•Temperatures

❱ Sample prep:•SpE, protein precipitation or LL extraction?•Choice of sample prep conditions

❱ Lab sessions:•mS method development for peptides and proteins•LC method development for peptides and proteins

For the practical part we will use UpLC and Tandem Quadrupole mS

Daily eventsTheory lessons and practice in the Lab.

Who Should AttendAnalysts who need knowledge of quantification of peptides and proteins with LC/mS/mS.

Prerequisites Attendees need previous experience of LC/mS.


SQD 2 Operation and Maintenance


ref.: 750000620 – 3 days

Course DescriptionComprehensive SQD 2 operation training course for small molecule applications. Hands-on massLynx software training, in addition to laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees on SQD 2 systems. Coverage of advanced topics, software options and hardware options dependent on participant interest and available time.

Topics Covered ❱ SQD 2 instrument overview ❱ Atmospheric pressure Ionization (ApI) Fundamentals ❱ Hardware ❱ Using the instrument console ❱ ACQUITY monitoring and control. ❱ IntelliStart™ ❱ Sample Tune ❱ Develop methods ❱ Calibration ❱ massLynx overview ❱ Creating ACQUITY methods ❱ Creating mS methods ❱ Creating Sample Lists ❱ Data Acquisition ❱ Data processing ❱ SQD 2 maintenance ❱ Laboratory sessions ❱ Tuning and Calibration ❱ Data Acquisition ❱ Quantification ❱ maintenance





SYNAPT G2-S (Biopharmaceutical)


ref.: 750000609 – 5 days

Course DescriptionA comprehensive ACQUITY UpLC / SYNApT G2-S operation training course for biopharmaceutical applications. Hands-on massLynx and BiopharmaLynx training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters SYNApT G2.

Topics Covered ❱ ToF Accurate mass overview•ToF theory•Use of accurate mass data

❱ Instrument optimization, data acquisition•SYNApT G2 instrument Schematics•Electrospray ionization•LockSpray™ ion source•IntelliStart•Tuning and calibration•mS and mS/mS data acquisition•mSE data acquisition

❱ routine maintenance ❱ massLynx software overview ❱ UpLC/mS Interfacing ❱ BiopharmaLynx•Intact protein spectrum denconvolution with maxEnt™

❱ Sample preparation (de-salting etc.). ❱ HDmS – acquisition of data using ion mobility•DriftScope


Who Should Attendpersonnel responsible for biopharmaceutical applications using SYNApT G2.



SYNAPT G2-S (NanoFlow Proteomics)


ref.: 750000627 – 5 days

Course DescriptionA comprehensive nanoACQUITY UpLC / SYNApT G2-S operation training course for proteomics applications. Hands-on proteomics workflow training, including laboratory sessions and IdentityE, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters SYNApT G2.


❱ Instrument optimization, data acquisition•SYNApT G2 instrument schematics•Electrospray Ionization•NanoLockSpray™ ion source•Tuning and calibration•mS and mS/mS data acquisition (including DDA)•mSE data acquisition

❱ routine maintenance ❱ massLynx software overview ❱ nanoACQUITY UpLC/mS Interfacing•NanoFlow electrospray

❱ Sample preparation ❱ Database Searching•Introduction to databanks•proteinLynx Global SErVEr™•IdentityE (basic introduction)* * This course gives a basic introduction to IdentityE. A dedicated course is available for full training on IdentityE and ExpressionE


Who Should Attendpersonnel responsible for proteomics applications using nanoACQUITY UpLC coupled to SYNApT G2.





SYNAPT G2-S (Small Molecules)


ref.: 750000610 – 5 days

Course DescriptionA comprehensive ACQUITY UpLC / SYNApT G2-S operation training course for small molecule applications. Hands-on massLynx training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters SYNApT G2.


❱ Instrument optimization, data acquisition•SYNApT G2 instrument Schematics•Electrospray Ionization•LockSpray Ion source•IntelliStart•Tuning and calibration•mS and mS/mS data acquisition•mSE data acquisition

❱ routine maintenance ❱ massLynx Software overview ❱ Basic training in:•metaboLynx – metabolite ID software•markerLynx – metabonomics software•ChromaLynx – screening software•massFragment™ *course content depends on attendees requirements

❱ UpLC/mS Interfacing ❱ HDmS – acquisition of data using ion mobility•DriftScope


Who Should Attendpersonnel responsible for small molecule applications using SYNApT G2.



Xevo G2-S Tof / Q-Tof (Biopharmaceutical)


ref.: 750000755 – 4 days

Course DescriptionA comprehensive ACQUITY / Xevo G2-S Q-Tof and Xevo G2-S Q-Tof operation training course for small molecule applications. Hands-on massLynx training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters Xevo G2-S Tof / Q-Tof.

Topics Covered ❱ ToF Accurate mass overview•ToF theory•Using accurate mass data

❱ Instrument optimization, data acquisition•Xevo G2-S Tof / Q-Tof hardware overview•Electrospray ionization•LockSpray ion source•IntelliStart•Tuning and calibration•mS and mS/mS data acquisition •mSE data acquisition

❱ routine maintenance ❱ massLynx software overview ❱ UpLC/mS Interfacing ❱ BiopharmaLynx•Intact protein spectrum denconvolution with maxEnt

❱ Sample preparation (de-salting etc.).


Who Should Attendpersonnel responsible for biopharmaceutical applications using Xevo G2-S Tof or G2-S Q-Tof.





Xevo G2-S Tof / Q-Tof (NanoFlow Proteomics)


ref.: 750000756 – 4 days

Course DescriptionA comprehensive nanoACQUITY UpLC / Xevo G2-S Q-Tof and Xevo G2-S Q-Tof operation training course for proteomics applications. Hands-on proteomics workflow training, including laboratory sessions and IdentityE, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters Xevo G2-S Tof / Q-Tof.



❱ routine maintenance ❱ massLynx software overview ❱ nanoACQUITY UpLC/mS Interfacing•NanoFlow electrospray

❱ Sample preparation ❱ Database Searching•Introduction to databanks.•proteinLynx Global SErVEr •IdentityE (basic introduction)* * This course gives a basic introduction to IdentityE. A dedicated course is available for full training on IdentityE and ExpressionE


Who Should Attendpersonnel responsible for proteomics applications using nanoACQUITY coupled to Xevo G2-S Tof or Xevo G2-S Q-Tof.



Xevo G2-S Tof / Q-Tof (Small Molecules)


ref.: 750000754 – 4 days

Course DescriptionA comprehensive ACQUITY / Xevo G2-S Q-Tof and Xevo G2-S Q-Tof operation training course for small molecule applications. Hands-on massLynx training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters Xevo G2-S Tof / Q-Tof.



❱ routine maintenance ❱ massLynx software overview ❱ Basic training in*:•metaboLynx – metabolite ID software•markerLynx – metabonomics software•ChromaLynx – Screening software•massFragment *course content depends on attendees requirements

❱ UpLC/mS Interfacing


Who Should Attendpersonnel responsible for small molecule applications using Xevo G2-S Tof or G2-S Q-Tof.





Xevo G2-S Tof / Q-Tof UNIFI (Biopharmaceutical)


ref.: 750000654 – 4 days

Course DescriptionA comprehensive ACQUITY / Xevo G2-S Q-Tof and Xevo G2-S Q-Tof operation training course for small molecule applications. Hands-on UNIFI training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters Xevo G2-S Tof / Q-Tof.


❱ Instrument optimization, data acquisition•Xevo G2-S Tof / Q-Tof hardware overview•Electrospray ionization•LockSpray ion source•Tuning and calibration•mS and mS/mS data acquisition •mSE data acquisition

❱ routine maintenance ❱ UNIFI software overview•focusing on biopharmaceutical applications

❱ UpLC/mS Interfacing ❱ Sample preparation (de-salting etc.).


Who Should Attendpersonnel responsible for biopharmaceutical applications using Xevo G2-S Tof or G2-S Q-Tof.



Xevo G2-S Tof / Q-Tof UNIFI (Small molecules)


ref.: 750000653 – 4 days

Course DescriptionA comprehensive ACQUITY / Xevo G2-S Q-Tof and Xevo G2-S Q-Tof operation training course for small molecule applications. Hands-on UNIFI training, including laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees and waters application experts on waters Xevo G2-S Tof / Q-Tof.


❱ Instrument optimization, data acquisition•Xevo G2-S Tof / Q-Tof hardware overview•Electrospray ionization•LockSpray ion source•Tuning and calibration•mS and mS/mS data acquisition •mSE data acquisition

❱ routine maintenance ❱ UNIFI software overview•focusing on small molecule applications

❱ UpLC/mS Interfacing


Who Should Attendpersonnel responsible for small molecule applications using Xevo G2-S Tof or G2-S Q-Tof.





Xevo TQD Operation and Maintenance

Language: EnglishLocation: waters European Training Center in manchester, United Kingdom

ref.: 750000611 – 4 days

Course Description Comprehensive Xevo TQD operation training course for small molecule applications. Hands-on massLynx software training, in addition to laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees on Xevo TQD systems. Coverage of advanced topics, software options and hardware options dependent on participant interest and available time.

Topics Covered ❱ Xevo TQD instrument overview ❱ Atmospheric pressure Ionization (ApI) Fundamentals ❱ Hardware ❱ Using the instrument console ❱ ACQUITY monitoring and control. ❱ IntelliStart ❱ Sample Tune ❱ Develop methods ❱ Calibration ❱ massLynx overview ❱ Creating ACQUITY methods ❱ Creating mS and mS/mS methods ❱ Creating Sample Lists ❱ Data Acquisition ❱ Data processing ❱ Quantification with TargetLynx and QUANpEDIA ❱ Xevo TQD maintenance ❱ Laboratory sessions ❱ Tuning and Calibration ❱ Data Acquisition ❱ Quantification ❱ maintenance



Xevo TQ-S (NanoFlow Proteomics)


ref.: 750000550 – 4 days

Course Description Comprehensive nanoACQUITY UpLC / Xevo TQ-S operation training course for quantification of protein/peptides using nanoESI on a tandem quadrupole. Hands-on massLynx software training, in addition to laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees on Xevo TQ-S systems. Coverage of advanced topics, software options and hardware options dependent on participant interest and available time.

Topics Covered ❱ Xevo TQ-S instrument overview ❱ Atmospheric pressure Ionization (ApI) Fundamentals ❱ Hardware ❱ Using the instrument console•nanoACQUITY UpLC monitoring and control•Tuning and Calibration•method development

❱ massLynx overview•Creating nanoACQUITY UpLC methods•Creating mS and mS/mS methods •Creating Sample Lists•Use of VerifyE

•Data Acquisition•Data processing•Quantification with TargetLynx and QUANpEDIA

❱ Xevo TQ mS maintenance ❱ Laboratory sessions•Tuning and Calibration•Data Acquisition•Quantification•maintenance

Who Should Attendpersonnel responsible for proteomic applications using nanoACQUITY UpLC coupled to Xevo TQ-S.





Xevo TQ-S (Small Molecules)


ref.: 750000549 – 4 days

Course Description Comprehensive Xevo TQ-S operation training course for small molecule applications. Hands-on massLynx software training, in addition to laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees on Xevo TQ-S systems. Coverage of advanced topics, software options and hardware options dependent on participant interest and available time.

Topics Covered ❱ Xevo TQ-S instrument overview ❱ Atmospheric pressure Ionization (ApI) Fundamentals ❱ Hardware ❱ Using the instrument console•ACQUITY monitoring and control•IntelliStart•Sample Tune•Develop methods•Calibration

❱ massLynx overview•Creating ACQUITY methods•Creating mS and mS/mS methods•Creating Sample Lists•Data Acquisition•Data processing•Quantification with TargetLynx and QUANpEDIA

❱ Xevo TQ mS maintenance ❱ Laboratory sessions•Tuning and Calibration•Data Acquisition•Quantification•maintenance

Who Should Attendpersonnel responsible for small molecules applications using Xevo TQ-S.



Xevo TQ-S UNIFI (Bioanalysis)


ref.: 750000655 – 4 days

Course DescriptionComprehensive Xevo TQ-S operation training course for small molecule applications. Hands-on UNIFI software training, in addition to laboratory sessions, enhances and reinforces lecture material. Laboratory sessions offer the opportunity to work with other attendees on Xevo TQ-S systems. Coverage of advanced topics, software options and hardware options dependent on participant interest and available time.

Topics Covered ❱ Xevo TQ-S instrument overview ❱ Atmospheric pressure Ionization (ApI) Fundamentals ❱ Hardware ❱ Using the instrument console ❱ ACQUITY monitoring and Control. ❱ Automated Compound optimisation ❱ Sample Tune ❱ Develop Analysis methods/ method Development ❱ Calibration ❱ UNIFI overview ❱ Creating an Analysis method ❱ Creating an Analysis ❱ Data Acquisition ❱ Data processing ❱ Quantification with UNIFI ❱ Xevo TQ-S maintenance ❱ Laboratory sessions ❱ Tuning and Calibration ❱ Data Acquisition ❱ Quantification ❱ maintenance





[ GENERAL CHROMATOGRAPHY ]

Fundamentals of HPLC

Language: Danish, EnglishLocation: Denmark, Sweden

ref.: 750000276 – 3 days

Course DescriptionDuring the course the different LC principles, techniques and separation modes will be covered. The course structure with lectures in the morning, and lab exercises in the afternoon.

Topics Covered ❱ HpLC theory ❱ HpLC instruments ❱ Straight and reversed phase chromatography ❱ Ion exchange chromatography ❱ Size exclusion chromatography ❱ Gradient elution ❱ method development ❱ Quantitation ❱ Sample preparation ❱ Troubleshooting and maintenance

Who Should AttendAll who want a basic understanding of HpLC.

PrerequisitesNone.


Fundamentals of HPLC (Theory)

Language: Danish, English, FinnishLocation: Denmark, Finland, Norway, Sweden, United Kingdom

ref.: 750000463 – 2 days

Course Description During the course the different LC principles, techniques and separation modes will be covered. This course is designed to help individuals enhance and expand upon their fundamental knowledge and understanding of liquid chromatography (HpLC). Investigation of various liquid chromatographic principles, techniques and separation modes will be discussed. Ultimately, an improved understanding of this technique will allow for informed decisions to be made in order to further enhance laboratory efficiency.

Course Topics ❱ HpLC theory ❱ HpLC instruments ❱ Normal and reversed phase chromatography ❱ Ion exchange chromatography ❱ Size exclusion chromatography ❱ Gradient elution ❱ method development ❱ Quantitation ❱ Sample preparation ❱ Troubleshooting and maintenance

Who Should AttendThis course is designed for laboratory personnel who are responsible for operating HpLC systems and require an enhancement/refreshment of their current HpLC skills.

PrerequisitesNone.



[ GENERAL CHROMATOGRAPHY ] [ GENERAL CHROMATOGRAPHY ]

HPLC Method Development with Lab

Language: Danish, EnglishLocation: Denmark, Finland, Sweden, United Kingdom

ref.: 750000164 – 3 days

Course DescriptionThe course will allow the participants to develop HpLC methods in a systematic way. participants will learn how to choosecolumns and mobile phases, and optimize the separations. Focus is on reversed phase separations, but the principles can beapplied to other separation modes. The course is structured with lectures in the morning, and lab. exercises in the afternoon.

Topics Covered ❱ reversed phase HpLC theory ❱ General guidelines for method development ❱ Column parameters and selectivity ❱ Systematic optimization of isocratic reversed-phase methods ❱ optimization of gradient methods ❱ method development software ❱ Laboratory exercises

Who Should AttendThis course is designed for people who want to expand their knowledge on HpLC method development.

PrerequisitesBasic HpLC knowledge.


HPLC Method Development (Theory)


ref.: 750000461 – 2 days

Course Description The course will allow the participants to develop HpLC methods in a systematic way. participants will learn how to choose columns and mobile phases, and optimize the separations. Focus is on reversed phase separations, but the principles can be applied to other separation modes.

Topics Covered ❱ General guidelines for method development ❱ reversed-phase column parameters ❱ Systematic method Development 1 ❱ Systematic method Development 2 ❱ optimising Gradient Separations ❱ reversed-phase Column Selectivity ❱ method optimisation Software techniques

Who Should AttendThis course is designed for people who want to expand their knowledge on HpLC method development.

PrerequisitesBasic knowledge of HpLC.




Troubleshooting Common HPLC Problems


ref.: 750000168 – 1 day

Course Description This course covers many commonly encountered HpLC problems. It will assist the chromatographer in logically identifying problems during the operation of his HpLC instrument, as well as identifying and preventing potential problems, thus reducing future downtime. Chromatographic examples are shown to make problem identification more clear.

Topics Covered ❱ problem prevention•potential sources of Chromatographic problems•Troubleshooting strategy

❱ performance monitoring•monitoring pressure•Determining system band spreading•Column efficiency testing•Tubing and connections

❱ Troubleshooting common problems•peak shape problems•retention time problems•Column problems•Chemistry problems•Baseline problems•Instrumentation problems•Vial and sample problems

❱ Column protection

Who Should AttendThe course is intended both for those developing new HpLC methods, and those who use HpLC instrumentation on a daily basis.



Practical LC Method Development with Lab


ref.: 750000650 – 3 days

Course descriptionThis three-day course will allow the participants to develop HpLC or UpLC methods in a systematic way. participants will learn how to choose columns and mobile phases, and optimize the separations. Focus is on reversed phase separations, but the principles can be applied to other separation modes. The course is structured with lectures in the morning and laboratory exercises in the afternoon using HpLC and/or UpLC systems.

Topics covered ❱ reversed phase HpLC theory ❱ General guidelines for method development ❱ Column parameters and selectivity ❱ Systematic optimization of isocratic reversed phase methods ❱ optimization of gradient methods ❱ method development software ❱ Laboratory exercises

Who Should AttendThis course is designed for people who want to expand their knowledge on HpLC or UpLC method development.


Course NotesEach attendee will receive a course manual to keep for future reference and a Certificate of Attendance



Waters Service Plans

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n Get preferred service and support

n Minimize costly downtime

n Control repair and maintenance costs

n Receive discounts on training courses

The right choice to increase laboratory productivity maintain peak performance and lower cost of ownership.

Learn more at

www.waters.com/serviceplans

[ EUROPEAN COURSES]

80

[ EUROPEAN COURSES ]

we are offering a lot of specialised mS, Data courses and SFC courses in our European Training Centers: manchester (UK), Frechen (Germany) or Saint-Quentin-en-Yvelines (France).

Visit www.waters.com/courses for more information on these trainings.

PART NUMBER DURATION COURSE TITLE

LABORATORY INFORMATICS > Saint-Quentin-en-Yvelines (France)

750000846 3 days Empower Software Enterprise System Administration and maintenance

750000575 1 day Empower Software Database System Administration and maintenance (optional)

> Frechen (Germany)

750000377 3 days NuGenesis SDmS System Administration

750000315 2 days NuGenesis SDmS and SDmS Vision publisher Software Development Kit

750000741 3 days NuGenesis 8 Lab Execution method Editor

750000739 3 days NuGenesis 8 ELN Administrator

750000740 3 days NuGenesis 8 Advance System Administration and Infrastructure

750000738 3 days NuGenesis 8 SDmS Administration

750000346 3 days SDmS Vision publisher / eLab Notebook System Administration

MASS SPECTROMETRY > Saint-Quentin-en-Yvelines (France)

750000307 4 days purification of Compounds using FractionLynx (mass Detector)

750000433 3 days purification of Compounds using FractionLynx (UV Detector)

SUPERCRITICAL FLUID CHROMATROGRAPHY (SFC) > Saint-Quentin-en-Yvelines (France)

750000553 1 day Introduction to Supercritical Fluid Chromatography (SFC)

750000556 2 days Supercritical Fluid Chromatography (SFC) purification with ChromScope

750000557 3 days Supercritical Fluid Chromatography (SFC) purification with FractionLynx

Waters Application Training and Support

Waters application specialists are available on-site or remotely to provide in-depth assistance with data interpretation and method development. T hey will familiarize your staff with instrument or software and provide guidance on application development.

Most popular application training offerings:n Pesticide Residue Analysis

n BioPharmaceutical – Intact Proteins, Oligos

n Clinical Applications – TDM and Pain Management

n Pharmaceutical – Structural Elucidation, Metabolite ID, QA/QC testing, Quantitation

n Proteomics


www.waters.com/applicationtraining

[ ON-SITE EDUCATION ]

Waters On-site training

Maximize learning at your location. Our existing training programs can be delivered “as is” or tailored to your specific laboratory challenges. By bringing a Waters Training Course to your site, not only will you select convenient dates to train your group, but you will also reduce or eliminate travel costs.

Benefits of on-site training:n Perfect choice for laboratories that need to train

groups of employees – from 5 to 500 or more.

n Get a maximum number of employees trained in new skills or technology in a minimal amount of time for one flat fee.

n Train your entire group of users in a familiar, confidential environment.

n Create synergy to the learning experience as lectures, hand-on sessions stay relevant to your laboratory needs.

n Eliminate your staff’s travel costs and minimize time away from the laboratory.

n Leverage serious Waters brainpower – our certified instructors are not only experts; they’re talented teachers delivering maximum skills in the allotted time.


www.waters.com/onsite

Start planning a course at your site today!

[ eLEARNING ]

PART NUMBER COURSE TITLE

General General LC eLearning Track750000657 module 1 Solvent Considerations750000658 module 2 Binary vs. Quaternary Gradient Systems750000659 module 3 LC Contamination Issues

LC/MS Principles and Concepts eLearning Track750000660 module 1 LC/mS Introduction750000661 module 2 Ionization modes750000662 module 3 mass Analyzers750000663 module 4 LC method Development750000664 module 5 Quantification using mS

Empower Empower 3 Workflow eLearning Track750000665 module 1 Empower 3 - Login750000666 module 2 Empower 3 - method Setup750000667 module 3 Empower 3 - Sample Acquisition750000668 module 4 Empower 3 - Data review750000669 module 5 Empower 3 - Edit Features750000670 module 6 Empower 3 - reports

Empower 3 in a Regulated Lab eLearning Track750000671 module 1 Empower 3 - Empower Access750000672 module 2 Empower 3 - System Audit Trail750000673 module 3 Empower 3 - project Audit Trail750000674 module 4 Empower 3 - Data management750000675 module 5 Empower 3 - Electronic Sign off750000676 module 6 Empower 3 - Data Security750000677 module 7 Empower 3 - Backup and restore

HPLC HPLC eLearning Track750000678 module 1 Theory of HpLC/TUV750000679 module 2 operation of HpLC/TUV750000680 module 3 maintenance of HpLC/TUV750000681 module 1 Theory of HpLC/pDA750000682 module 2 operation of HpLC/pDA750000683 module 3 maintenance of HpLC/pDA

UPLC UPLC eLearning Track750000685 module 1 Theory of UpLC/TUV750000686 module 2 operation of UpLC/TUV750000687 module 3 maintenance of UpLC/TUV750000688 module 1 Theory of UpLC/pDA750000684 module 3 maintenance of UpLC/pDA750000689 module 2 operation of UpLC/pDA

Fundamentals Fundamentals eLearning Track750000690 module 1 Fundamentals of LC/mS750000691 module 2 Basic massLynx Functions

Instrumentation MS Instrumentation eLearning Track750000692 module 1 IntelliStart: Quadrupole-Based mS Systems750000693 module 2 IntelliStart: Q-Tof-Based mS Systems750000694 module 3 Quanoptimize750000695 module 4 Elemental Composition

PART NUMBER COURSE TITLE

TargetLynx TargetLynx eLearning Track750000696 module 1 TargetLynx - overview750000697 module 2 TargetLynx - method Setup750000698 module 3 TargetLynx - Data processing and review750000699 module 4 TargetLynx - Advanced Topics

MarkerLynx MarkerLynx eLearning Track750000700 module 1 markerLynx - overview & method Setup750000701 module 2 markerLynx - Data processing and review

ChromaLynx ChromaLynx eLearning Track750000702 module 1 ChromaLynx - Non-Targeted Analysis750000703 module 2 ChromaLynx - Targeted Analysis750000704 module 3 ChromaLynx - Libraries

MetaboLynx MetaboLynx eLearning Track750000705 module 1 Drug metabolism overview750000706 module 2 metaboLynx - method Setup750000707 module 3 metaboLynx - Data processing and review750000708 module 4 metaboLynx - Advanced Topics

MassLynx MassLynx eLearning Track750000709 module 1 massLynx Architecture750000710 module 2 massLynx Data Acquisition750000711 module 3 massLynx Data processing and review - Chromatogram750000712 module 4 massLynx Data processing and review - Spectrum750000713 module 5 massLynx Data processing - Strip Functions

Intact Protein Analysis Intact protein Analysis750000714 module 1 Intact protein - Instrument Setup and Data Acquisition750000715 module 2 maxEnt 1 and Transform750000716 module 3 BiopharmaLynx (Intact protein) - method Setup & processing750000717 module 4 BiopharmaLynx (Intact protein) - Data Analysis

Peptide Mapping Analysis Peptide Mapping Analysis750000718 module 1 peptide map - Instrument Setup and Data Acquisition750000719 module 2 maxEnt 3 and De Novo Sequencing750000720 module 3 BiopharmaLynx (peptide map) - method Setup & processing750000721 module 4 BiopharmaLynx (peptide map) - Data Analysis

eLearning Track UNIFI Core Topics eLearning Track750000722 module 1 UNIFI overview750000723 module 2 UNIFI Administration750000724 module 3 UNIFI Instrument Systems and Configuration750000725 module 4 UNIFI Data Acquisition750000726 module 5 UNIFI Data review750000727 module 6 UNIFI reporting750000728 module 7 UNIFI Signature methods750000729 module 8 UNIFI Scientific Library

These courses will be available in the course of 2013.


[ eLEARNING ] [ eLEARNING ]

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iRequest Phone Support

Get technical phone support on Waters LC, MS and SFC instruments and software.(Serial # or Support ID required.)

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Ask a Field Service engineer to be dispatched for Performance Maintenance, Repair Visit, Qualification, or Relocation.(Serial # or Support ID required.)

iRequest On-site Training

Request information on training based on your group size, schedule, and budget.(Login to Waters account required.)

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[ REGISTRATION ]

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phone: 0208 238 6100 [email protected]

For reGistration, location, Further details, Please visit our webiste

W W W.WATERS.COM/COURSES

Waters Corporation European Headquarters and Training Center Bp 60878056 Saint-Quentin-en-Yvelines, France

phone 33 1 30 48 72 00Fax 33 1 30 48 72 01

Waters MS Technology Centre Waters GmbHmicromass Atlas park, Simons way Europaallee 27–29manchester, m22 5pp, United Kingdom 50226 Frechen, Germany

phone 44 161 435 4100 phone 49 2234 9207 0Fax 44 161 435 4444 Fax 49 2234 9207 99

Ireland and Northern Ireland

waters Ireland Block 3.1 woodford Business park Santry Dublin 9

phone: +353 (0) 1 448 1500 Fax: +353 (0) 1 448 1510

Denmark and Norway

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phone: +45 46 59 80 80 [email protected]

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phone:+358-9-56596288 [email protected]

Sweden

po Box 485 191 24 Sollentuna, Sweden

phone: +46 8 555 115 00 [email protected]

Documents

accelerate your technology deployment · Quantification of peptides and proteins by LC/mS/mS 56 ... This course provides a hands-on introduction to the new features and enhancements