ABO BLOOD GROUPING (REVERSE)
ABO BLOOD GROUPING (REVERSE)
REVERSE GROUPINGPrinciple:Detection of antibodies in the
patients serum with known commercial antisera.Summary of
Results:
Blood GroupAgglutination with A cellsAgglutination with B
cellsA-+B+-AB--O++
REVERSE GROUPING
Guide Questions:1. What is the importance of reverse grouping?
Confirms the reaction obtained by the forward grouping test
2. What are the limitations of the reverse groupings?Cannot
detect Rhesus antibodiesFalse negative reactions in the reverse
type may be due to weak or missing antibodiesFalse positive
reactions may be due to those antibodies other than anti-A or
anti-B.
Post-Lab Conference Questions:1. List some precautions in
performing the reverse type. Explain each. Always drop clear
solutions first, RBCs second.Red blood cell reagents be allowed to
warm at room temperature prior to use.Use the recommended strength
of cell suspension
Post-Lab Conference Questions:2. Discuss the difference between
antiserum and patient ABO antibodies. ABO antibodiesAntibodies have
been described as naturally occurring because they are produced
without any exposure to RBC
AntiserumAntiserum is blood serum containing polyclonal
antibodies. These polyclonal antibodies are antibodies that are
secreted by different B cell lineages within the body.
