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AA Message from the Organizers Message from the Organizers
August 2003 Dear Participants, Colleagues and Friends, It is a pleasure to welcome you to the 6th Conference on Protein Expression in Animal Cells (6th PEACe). The conference is being held this year in the breathtaking International Ski Resort of Mt Tremblant situated in the magnificent Laurentian mountains of Québec. The 6th PEACe conference follows in a series of International conferences which started more than 10 years ago as the Baculovirus and Insect Cell Conference. This then expanded to include the broad spectrum of gene delivery systems for recombinant protein expression. The present conference, the 6th in the series, reflects the wide reach that the conference now has in terms of including more than one gene expression system and was thus renamed more inclusively as Protein Expression in Animal Cells. The conference will provide an excellent opportunity for experts from industry and academia to meet and exchange ideas in the idyllic surroundings of the Laurentian mountain resort. The meeting promises to be exciting and stimulating with a wide range of topics ranging from gene delivery to the equality of the recombinant protein. We thank the more than 200 participants who have contributed to the conference by presenting their work as oral or poster presentations. The participants represent a global community and emphasize the International flavor of this gathering. The conference has been structured to facilitate the exchange of information including both formal presentations during the Sessions and time for informal discussions and social activities. I take this opportunity to thank the members of the International and Local organizing committees for their support and collaboration to make this conference a success and invite all the participants to enjoy the scientific exchange, the social activities and the beauty of the Laurentian mountains during the conference. On behalf of the organizing committee, I wish you all a pleasant and memorable conference experience.
Dr. Amine A. Kamen.
6th PEACe Organization6th PEACe Organization Scientific Advisory Committee Mike Betenbaugh – Chemical Engineering Department, John Hopkins University, Baltimore,
MD, USA Michael Butler – Department of Microbiology, University of Manitoba, Winnipeg, Canada Reingard Grabherr - Institute of Applied Microbiology, University of Natural Resources and life
Sciences, Vienna, Austria Amine Kamen – Biotechnology Research Institute, National Research Council Canada (BRI-
NRC), Montréal, QC, Canada. Tom Kost – GlaxoSmithKline, Research Triangle Park, NC, USA Kenneth Lundstrom – BioXtal, Lausanne, Switzerland Balwant Patel – Roche Applied Science, Indianapolis, IN, USA Girish Shah – GlaxoSmithKline, Hertfordshire, UK
Local Organizing Committee (BRI-NRC, Montréal, QC, Canada) Marc Aucoin Parminder Chahal Jocelyne Côté Yves Durocher Cynthia Elias Amine Kamen Marie-Odile Martin Robert Voyer- Local Supporting Committee Ani Armenian (BRI-NRC, Montréal, QC, Canada), Lucille Beaudet (PerkinElmer Life Sciences, Montréal, QC, Canada), Martina Bielefeld-Sevigny (DSM Biologics, Montréal, QC, Canada), Alain Garnier (Université Laval, Québec City, QC, Canada), Denis Groleau (BRI-NRC, Montréal, QC, Canada), Mario Jolicoeur ( École Polytechnique de Montréal, Montréal, QC, Canada), Claude Larose (Qbiogene, Montréal, QC, Canada), Bernard Massie (BRI-NRC, Montréal, QC, Canada), Michel Perrier (École Polytechnique de Montréal, QC, Canada), Steve Xanthoudakis (Merck Frosst, Montréal, QC, Canada). Conference Logistics Hospitalité Québec, (Québec City, QC, Canada)
Major Sponsors
National Research Council Canada
Biotechnology Research Institute - National Research Council Canada
Ministère du Développement économique et régional Québec
Natural Sciences and Engineering Research Council of Canada
Canadian Institutes of Health Research
Institute of Genetics - Canadian Institutes of Health Research
Roche Applied Science
Amgen Inc.
Kendro Laboratory Products
Invitrogen Corporation
Merck Frosst Canada Ltd. - Merck & Co., Inc.
DSM Biologics
Supporting Sponsors
GlaxoSmithKline
Abbott Laboratories, Limited
Wisent Inc.
Université Laval
CREFSIP: le centre de recherche sur la fonction, la structure et l'ingénierie des protéines
We thank all our sponsors for their financial support and would also like to acknowledge Protein Science (a Cold Spring Harbor Laboratory Press publication), Qbiogene Inc. and the Wilbio Bioprocessing Journal for their “in-kind” contributions.
6th PEACe 2003- NRC Poster Award6th PEACe 2003- NRC Poster Award
The National Research Council of Canada Poster Award will be given in recognition of the best poster presented at the 6th Protein Expression in Animal Cells meeting. The presentation of this award aims to encourage the greater participation of the entire scientific and especially the academic community. The award will be given to the poster which best meets the criteria of the judges based on originality, relevance and quality of presentation.
Award An award of 1000 $CDN and a certificate will be given to the Best Poster. The award will be presented to the first author of the winning poster, at the Gala Dinner, by Dr Peter Hackett, Vice President, NRC, Canada. Eligibility The Best Poster Award is open to all registered conference attendees. The award recipient must have presented the poster at the conference poster session. Evaluation Posters will be judged by either the poster session chairs or others to be selected from conference attendees, as necessary. Criteria Posters will be judged based on originality, experimental approach/ progress to-date, significance to the advancement of the field of the conference topic, and overall quality. Factors to consider in judging include the following:
• Originality: Does the work represent a novel approach to the problem presented, either theoretically or experimentally?
• Relevance: Does the work represent a substantial contribution to the conference topic? Can the conclusions be made relevant to a variety of applications?
• Overall quality: Is the poster well-presented both visually and orally? Is the organization clear and easy to follow?
Travel to Mt. Tremblant
The nearest international airport is Dorval Airport, Montréal, Québec.
From the airport any one of the following can be used to travel to Fairmont Tremblant, Mt. Tremblant.
Limousine
• Please contact Concierge for arrangements and prices.
• Rates: One way approximately $250.00, taxes and service not included
• Taxi Rates: Approximately $225.00, taxes and service not included
Rental Car
Hertz: 1 (800) 263-0600
• Available through the Concierge
• AVIS: 1 (800) 879-2847; Tilden: 1 (800) 387-4747 ; Budget: 1 (800) 268-8900 Thrifty: 1 (800) 367-2277
• All the above numbers can be reached from Canada and the U.S.
Driving Directions from Dorval and Area Map
From Dorval Airport (140 km about 1 hour 45 minutes):
1. Take Hwy 20 East (Montreal downtown) for about 5 km.
2. Then take Hwy 13 North toward St-Jérôme.
3. From there, take Hwy 640 East toward St-Jérôme.
4. Then take Hwy 15 North for about one hour.
5. Hwy 15 will change into Hwy 117 at Ste-Agathe.
6. Continue on Hwy 117 North until St-Jovite.
7. Continue on Hwy 117 North and take the exit on your right for Tremblant.
8. Turn on your right hand side on Montée Ryan.
9. Follow Montée Ryan until the end to Lake Tremblant.
10. At Lake Tremblant, turn on your right hand side on Chemin de la Chapelle and until you reach the hotel.
Tourist Attractions in Montréal
Tourist centres The main tourist information centre in Montreal is located near the corner of Peel and Ste-Catherine at 1001, rue du square Dorchester (corner Metcalfe), metro Peel, 514-873-2015 or 1-877-266-5687. It is open 7 a.m.-8 p.m. from June to early September; 7:30 a.m.-6 p.m. in May and September-October, and 9 a.m.-6 p.m. from November till April. The Old Montreal Tourist Centre at 174 Notre-Dame East is open 9 a.m.-5 p.m. except from late June to early October when it's open till 7 p.m. Montréal’s main Attractions : Bateau-Mouche (cruises) at the Old Port of Montréal: Experience Montréal as never before, aboard Le Bateau-Mouche! Charting a course inaccessible to conventional watercraft, this elegant Parisian riverboat offers you breathtaking views of Montréal and its skyline from a unique vantage point. Sign up for a guided tour, or make reservations for dinner on the river. Casino de Montréal: Formerly the French and Québec pavilions of Expo ’67, the Casino de Montréal offers over 3,060 slot machines, 120 gaming tables and a dazzling atmosphere. The Casino is also home to the Cabaret, a spectacular 500-seat performance hall dedicated to world-class variety shows, and four restaurants sure to suit every taste, from a quick snack to an exquisite gourmet experience. Environment Canada's Biosphère: This Expo '67 American pavilion is Canada’s first Ecowatch Centre and remains a Montréal architectural masterpiece. The only museum of water in North America, the Biosphère is principally dedicated to the St. Lawrence River and the Great Lakes, along with an emphasis on better understanding and protecting these invaluable resources. Thematic and interactive exhibition halls, multimedia presentations, youth activities, modern museography and an experienced team of interpretive guides make the Biosphère an educative and entertaining place for the entire family. Group tours and room rentals available. Jet Boating and Rafting the Lachine Rapids: Unparalleled Jet Boating ride on the Lachine Rapids. Wet, wild and wonderful. Lunch and dinner packages are available for groups of 10 persons and over. Also available, Speed Boating, Rodeo style jet boating, enough action to resemble a chase in a James Bond movie. Rafting: initiation. Montréal Biodôme: Experience ecology in the heart of Montréal! Four natural ecosystems create an environmental museum inside the Biodôme. Rocky landscapes, tumbling waterfalls and majestic trees are home to birds, mammals and fish that fly, climb and swim through their natural habitats: the tropical forest, the Laurentian forest, the Saint-Laurent marine environment and the polar world. Montréal Botanical Garden: The Montréal Botanical Garden, established in 1931, is considered to be one of the world’s major botanical showcases. Its 10 exhibition greenhouses and over 30 gardens abound with the colours and fragrances of the world and boasts more than 21,000 species and varieties of plants. The Chinese and Japanese Gardens offer exotic landscapes, whereas the Tree House displays
Québec’s abundant forest wealth. In the First Nations Garden, you can discover the relationship the 11 Amerindian and Inuit nations of Québec have always maintained with the world of plants. Montréal Insectarium(together with botanical garden): The Insectarium invites you to discover the fascinating world of insects. This museum houses a prestigious collection of several thousand insects from every corner of the earth. In summer, come discover our splendid butterfly garden that will illustrate, in all its beauty, how to form new ties with nature. Mosaiculture International Montréal 2003 – Myths and Legends of the World: These superb floral sculptures, some of them 10 metres high, have dazzled over 1.3 million visitors in the past two years. Come see the new generation! The last international mosaiculture competition in Montréal. Old Montréal: Located between the river and the city-centre, Old Montréal and the Old Port will take you on a historic tour of a great North American metropolis. Something quite unique is that so much of the past is still visible today – from the place where the city was founded in 1642, to physical evidence of all periods, such as the French colony’s winding paths, remarkable 18th and 19th century architecture and the first Canadian skyscrapers. More romantic and charming than ever, since architectural lighting has helped highlight the Old Port and Old Montréal’s rich past, its museums, boutiques, sidewalk cafés and public places are alive with activities all year long. Saint Joseph's Oratory of Mount Royal: Saint Joseph’s Oratory atop Mount Royal is one of the world’s most visited shrines. The basilica’s huge dome reaches 97 metres and is second only in height to Saint Peter’s basilica in Rome. The sheer size of it contrasts sharply with its frail founder, Brother André, the humble doorkeeper who had initiated its construction in 1904. The shrine devoted to Saint Joseph consists of a primitive chapel, a votive chapel, a crypt and a basilica large enough to accommodate 10,000 worshippers. Relics of Brother André and many ex-voto of miraculously cured pilgrims are exhibited. The basilica’s great organs and 52 bell chimes will occasionally rekindle solemn works of great composers. (This information has been provided Courtesy of Tourisme Québec. Details on these and other attractions are available on their website- http://www.tourisme-montreal.org)
General Notes
Audiotaping, vidoetaping and photographing during the presentations is prohibited. Please turn cellular phones off or set to vibrate during the sessions. Speakers: Please note that a 5 min question period is included in the time allowed for the presentation. Poster Session: Posters should be displayed before the commencement of the first session on Monday 8th September 2003. The number of the poster can be found in the Abstract book provided at the time of registration.
Sumit Chanda (10:40 - 11:10)Genomics Institute of the Novartis Research Foundation, San
Diego, California, U.S.A.Genomic-scale functional annotation through modulation of gene
expression
Inte
gra
l Tra
nsm
emb
ran
e P
rote
ins
and
GP
CR
s
Jeff Clare (10:50 - 11:20)Department of Gene Expression and Protein Biochemistry,
GlaxoSmithKlineSolving the challenges of ion channel expression for drug
discovery
An
tib
od
ies
and
oth
er S
ecre
ted
Pro
tein
s
Jim Michaels (10:50 - 11:20)Bayer Biological Products, Berkeley, California, U.S.A.
Perfusion cell culture process development: Platform technologies and automation
Amine Kamen (11:10 - 11:30)Biotechnology Research Institute, National Research Council Canada,
Montreal, Quebec, CanadaClosing Remarks Conference EndsChristopher G. Tate (11:20 - 11:50)
MRC Laboratory of Molecular Biology, Cambridge, U.K.Comparison of seven different heterologous protein
expression systems for the production of the serotonin transporter
Gene Lee (11:20 -11:50)Wyeth BioPharma, Andover, Massachusetts, U.S.A.
High-level expression of a difficult to express soluble receptor:Fc fusion protein in CHO cells
RETURN TO MONTREAL AND
SITE VISITS TO THE BIOTECHNOLOGY RESEARCH INSTITUTE OF
CANADA AND DSM BIOLOGICS
(16:30 - 18:30)
Hig
h-T
hro
ug
hp
ut S
trat
egie
s
Danial Wayner (9:00-9:40)Steacie Institute for Molecular Sciences, National Research
Council of Canada, Ottawa, Ontario, CanadaKeynote Lecture
Robert Kotin (9:30- 10:00)National Institute of Health, Bethesda, Maryland, U.S.A.Production of Adeno-Associated Virus Vectors in Insect
Cells
Alex van der Eb (9:30- 10:00)Sylvius Laboratory, Leiden University Medical Center,
Leiden, The NetherlandsIsolation of Adenovirus E1-transformed human cell lines: PER.C6TM as a platform for the production of proteins
Douglas Conklin (9:30 - 10:00)Cancer Genome Center, Cold Spring Harbor, New York, U.S.A.
Applications of RNAi in MammalsJennings Worley (9:40 - 10:10)
Amphora Discovery Corp., Durham, North Carolina, U.S.A.Cell-based assays in the microfluidic environment
BREAK (10:00 - 10:30)
Noelle-Ann Sunstrom (10:00 - 10:20)University of New South Wales, Australia
A novel expression system for recombinant protein production in CHO cells.
Adiba Ishaque (10:00 - 10:20)Bayer Corporation, Berkeley, California, U.S.A.
Over-expression of a chaperone protein in recombinant BHK-21 cells increases cell viability and FVIII productivity by conferring
resistance to apoptosis.
BREAK (10:20 - 10:50) BREAK (10:20 - 10:50)
Thursday September 11, 2003BREAKFAST (7:00-8:30) BREAKFAST (7:00-8:30) BREAKFAST (7:00-8:30)
BREAKFAST (7:00-9:00)
Bac
ulo
viru
s an
d In
sect
Cel
l Tec
hn
olo
gy
Amine Kamen (8:30 - 9:00)Biotechnology Research Institute, National Research
Council Canada, Montreal, Quebec, CanadaInsect Cell Technology: a robust and versatile gene
expression platform
Cel
l En
gin
eeri
ng
I
Michael Kallos (8:30 - 9:00)Pharmaceutical Production Research Facility, University of
Calgary, Calgary, Alberta, CanadaLarge-scale expansion of clinically relevant quantities of mammalian neural stem cells in suspension bioreators
Raymond Stevens (10:10 - 10:40)The Scripps Research Institute, La Jolla, California, U.S.A.Design of high-throughput protein production methods for
structural biology
Sunday September 7, 2003 Monday September 8, 2003 Tuesday September 9, 2003 Wednesday September 10, 2003
LUNCH (11:50 - 13:20)
Kenneth Lundstrom (11:50 - 12:20)BioXtal, Lausanne, Switzerland
Expression and structural genomics on G Protein-Coupled Receptors
James Piret (11:50 - 12:20)Biotechnology Laboratory, University of British Cloumbia,
Vancouver, British Columbia,CanadaThe rush to high expression: Rock of Sisyphus or targeted
development?
Alexandra Spenger (11:30 - 11:50)Institute of Applied Microbiology, University of Natural Resouces and Applied Life Sciences, Vienna, Austria
Comparing promoter specific "gene silencing" effects of baculovirus delivered genes in mammalian cells
Kostas Iatrou (12:20 - 12:40)Institute of Biology, NCSR "Demokritos", Greece
Lepidopteran cell-based high throughput screens for the identfication of ligand mimetics for insect and mammalian
receptors
Dirk-Jan Opstelten (12:20 - 12:40)Crucell NV, Leiden, The Netherlands
The human cell line PER.C6TM as an expression platform for the production of recombinant antibodies
LUNCH (12:40 -13:30)
LUNCH (12:40-14:20)POSTER SESSION (13:20 - 17:00)
ACTIVITIES IN MONT-TREMBLANT OR MONTREAL
(13:30 -22:30)
PROGRAM AT A GLANCE
Cel
l En
gin
eeri
ng
II
Hansjorg Hauser (8:30 - 9:00)GBF, German Research Centre for Biotechnology, Germany
One-step selection of high-level recombinant protein expressing cells using a stop codon suppression strategy
Patrick Farrell (9:00 - 9:30)SYN-X Pharma, Inc., Mississauga, Ontario, Canada
An expression cassette for recombinant protein expression, baculovirus, manipulation, and high-
throughput screening for bioactive compounds in insect cells
Garry Nolan (9:00 - 9:30)Baxter Laboratory for Genetic Pharmacology, Stanford,
U.S.A.Single cell proteomic analysis of multiple intracellular
processes by high dimensional flow cytometry
Martin Fussnegger (9:00 - 9:30)ETH Institute of Biotechnology, ETH Hoenggerberg, Zurich,
SwitzerlandTranscription control engineering in mammalian cells and animals
Gen
e D
eliv
ery
by
Vir
al a
nd
No
n-V
iral
Vec
tors
I
Bernard Massie (10:30 - 11:00)Biotechnology Research Institute, National Research
Council Canada, Montreal, Quebec, CanadaGeneration of Adenoviral libraries for high throughput
functional studies and protein production
Tom Kost (11:00 - 11:30)GlaxoSmithKline, TRP, North Carolina, U.S.A.
Recombinant baculoviruses for mammalian cell gene delivery
Amine Kamen (19:40 - 20:00)Biotechnology Research Institute, National
Research Council Canada, Montreal, Quebec, Canada
(Welcome/Introduction to the Conference and Keynote Lecture)
Ronald Cobb (17:50 - 18:10)Berlex Biosciences, Richmond
High levels of protein expression using different mammalian CMV promoters in several cell lines
SOCIAL TIME (21:00 -22:30) SOCIAL TIME (21:00 -22:30) SOCIAL TIME (21:00 -22:30)
COCKTAILS (18:00 - 19:00)BREAK (18:10 - 19:00)
BREAK (18:30 - 19:00)
DINNER (19:00 - 21:00)
DINNER (19:00 - 21:00) GALA DINNER (19:00 - 21:00)
John Bergeron (20:00 - 20:40)Caprion Proteomics, Montreal, Quebec, Canada
Keynote LectureProteomics applications for biological paradigms
Stefanie Weikert (14:50 - 15:20)Genentech, San Francisco, California, U.S.A.
Modulation of effector functions by deleting the disulfide bonds in the hinge region of monoclonal antibodies
Mike Butler (15:20 - 15:50)University of Manitoba, Winnipeg, Manitoba, Canada
Culture parameters that cause perturbations in the glycosylation of proteins secreted by mammalian cells.
Pro
tein
Qu
alit
y
Rebecca McCoy (14:20 -14:50)Amgen, Seattle, Washington, U.S.A.
Heterologous protein expression in mammalian cells can lead to protein misfolding that is unrelated to cell specific productivity
BREAK (15:50 - 16:20)
WELCOME / REGISTRATION (16:00-19:00)
INDUSTRIAL WORKSHOPS(16:20 - 18:30)
Gen
e D
eliv
ery
by
Vir
al a
nd
No
n-V
iral
V
ecto
rs II
Jude Samulski (17:00 - 17:30)University of North Carolina, Chapel Hill, North Carolina,
U.S.A.Development of novel serotype specific AAV vectors for
gene therapy
Yves Durocher (17:30 - 17:50)Biotechnology Research Institute, National Research
Council Canada, Montreal, Quebec, CanadaLarge-scale transfection of HEK293 cells for the production of recombinant proteins and adeno-
associated viruses
RETURN TO MONTREAL AND
SITE VISITS TO THE BIOTECHNOLOGY RESEARCH INSTITUTE OF
CANADA AND DSM BIOLOGICS
(16:30 - 18:30)
ACTIVITIES IN MONT-TREMBLANT OR MONTREAL
(13:30 -22:30)
POSTER SESSION (13:20 - 17:00)
Conference Schedule
Sunday, September 7, 2003 4 : 00 –7 : 00 PM Registration/Cocktails 7 : 00 PM Dinner 7 : 40 PM Opening Remarks/Introduction to Keynote Speaker
Amine Kamen, Biotechnology Research Institute, National Research Council Canada, Montréal, Québec, Canada.
8 : 00 PM Keynote Lecture: : John Bergeron, Caprion Proteomics and Faculty of Medicine, McGill University, Montréal, Québec, Canada Proteomics applications for biological paradigms
9 : 00 – 10 : 00 PM Social Hour
Monday, September 8, 2003 7 : 00 – 8: 30 AM Breakfast 8 : 30 – 10: 00 AM Session 1: Baculovirus and Insect Cell Technology
Chair: Tom Kost, GlaxoSmithKline, Research Triangle Park, North Carolina, USA
8 : 30 – 9: 00 AM Amine Kamen, Biotechnology Research Institute, National Research Council Canada, Montréal, Québec, Canada.
Insect cell technology: A rapid and versatile gene expression platform. 9 : 00 – 9: 30 AM Patrick Farrell, Syn-X Pharma, Inc, Mississauga, ON, Canada.
An expression cassette for recombinant protein expression, baculovirus manipulation and high throughput screening for bioactive compounds in insect cells.
9 :30 – 10: 00 AM Robert Kotin, National Institute of Health, Bethesda, Maryland, USA. Production of adeno-associated virus vectors in insect cells. 10 :00 – 10: 30 AM Coffee Break 10 :30 – 11: 50 AM Session 2: Gene Delivery by Viral and Non-Viral Vectors I Chair: Kenneth Lundstrom, BioXtal, Lausanne, Switzerland.
10 :30 – 11: 00 AM Bernard Massie, Biotechnology Research Institute, National Research
Council Canada, Montréal, Québec, Canada. Generation of adenoviral libraries for high throughput functional studies and protein production
11 :00 – 11 :30 AM Tom Kost, GlaxoSmithKline, Research Triangle Park, North Carolina,
USA. Recombinant baculoviruses for mammalian cell gene delivery. 11 :30 – 11 :50 AM Alexandra Spenger, Institute of Applied Microbiology, University of
Natural Resouces and Applied Life Sciences, Vienna, Austria. Comparing promoter specific "gene silencing" effects of baculovirus
delivered genes in mammalian cells. 12 :00 – 1: 20 PM Lunch
1: 20 – 5: 00 PM POSTER SESSION 5: 00 – 6:10 PM Session 3: Gene Delivery by Viral and Non-Viral Vectors II Chair:Reingard Grabherr, Institute of Applied Microbiology,
University of Agricultural Sciences, Vienna, Austria. 5: 00 – 5 :30 PM Jude Samulski, University of North Carolina, Chapel Hill, North
Carolina, U.S.A. Development of novel serotype specific AAV vectors for gene therapy. 5: 30 – 5 :50 PM Yves Durocher, Biotechnology Research Institute, National Research
Council Canada, Montréal, Québec, Canada. Large-scale transfection of HEK293 cells for the production of
recombinant proteins and adeno-associated viruses. 5: 50 – 6: 10 PM Ronald Cobb, Berlex Biosciences, Richmond, CA, USA. High levels of protein expression using different mammalian CMV
promoters in several cell lines. 7: 00 – 9: 00 PM Dinner 9: 00 – 10: 30 PM Social Hour
Tuesday, September 9, 2003 7 : 00 – 8: 30 AM Breakfast 8 : 30 – 10: 20 AM Session 4: Cell Engineering I
Chair: Michael Butler University of Manitoba, Winnipeg, Manitoba, Canada.
8 : 30 – 9: 00 AM Michael Kallos, Pharmaceutical Production Research Facility, University of Calgary, Calgary, Alberta, Canada.
Large-scale expansion of clinically relevant quantities of mammalian neural stem cells in suspension bioreactors.
9 : 00 – 9: 30 AM Garry Nolan, Baxter Laboratory for Genetic Pharmacology, Stanford,
CA, USA. Single cell proteomic analysis of multiple intracellular processes by
high dimensional flow cytometry.
9 :30 – 10: 00 AM Alex van der Eb, Sylvius Laboratory, Leiden University Medical Center, Leiden, The Netherlands.
Isolation of Adenovirus E1-transformed human cell lines: PER.C6TM as a platform for the production of proteins.
10 :00 – 10: 20 AM Noelle-Ann Sunstrom, University of New South Wales, Australia A novel expression system for recombinant protein production in CHO
cells. 10 :20 – 10: 50 AM Coffee Break 10 :50 – 12: 40 PM Session 5: Integral Transmembrane Proteins and GPCRs
Chair: Michel Bouvier, University of Montréal, Québec, Canada.
10 :50 – 11: 20 AM Jeff Clare, Department of Gene Expression and Protein Biochemistry,
GlaxoSmithKline, Stevenage, Hertfordshire, UK Solving the challenges of ion channel expression for drug discovery 11 :20 – 11 :50 AM Christopher G. Tate, MRC Laboratory of Molecular Biology,
Cambridge, U.K. Comparison of seven different heterologous protein expression systems
for the production of the serotonin transporter 11 :50 – 12 :20 AM Kenneth Lundstrom, BioXtal, Lausanne, Switzerland. Expression and structural genomics on G Protein-Coupled Receptors 12 :20 – 12: 40 PM Kostas Iatrou, Institute of Biology, NCSR "Demokritos", Athens,
Greece. Lepidopteran cell-based high throughput screens for the identfication of ligand mimetics for insect and mammalian receptors.
12: 40 PM Lunch 1:30 PM Departure for Social Activities in Montreal/Mt Tremblant.
Wednesday, September 10, 2003 7 : 00 – 8: 30 AM Breakfast 8 : 30 – 10: 20 AM Session 6: Cell Engineering II
Chair: Mike Betenbaugh, John Hopkins University, Baltimore, Maryland, USA.
8 : 30 – 9: 00 AM Hansjorg Hauser, GBF, German Research Centre for Biotechnology, Braunschweig, Germany.
One-step selection of high-level recombinant protein expressing cells using a stop codon suppression strategy.
9 : 00 – 9: 30 AM Martin Fussenegger, ETH Institute of Biotechnology, ETH
Hoenggerberg, Zurich, Switzerland. Transcription control engineering in mammalian cells and animals.
9 :30 – 10: 00 AM Douglas Conklin, Cancer Genome Center, Cold Spring Harbor, NY, USA.
Applications of RNAi in mammals 10 :00 – 10: 20 AM Adiba Ishaque, Bayer Corporation, Berkeley, California, U.S.A. Over-expression of a chaperone protein in recombinant BHK-21 cells
increases cell viability and FVIII productivity by conferring resistance to apoptosis.
10 :20 – 10: 50 AM Coffee Break 10 :50 – 12: 40 PM Session 7: Antibodies and other Secreted Proteins
Chair: David Chang, IDEC Pharmaceuticals Corporation, San Diego, CA, USA.
10 :50 – 11: 20 AM Jim Michaels, Bayer Biological Products, Berkeley, California, USA.
Perfusion cell culture process development: Platform technologies and automation
11 :20 – 11 :50 AM Gene Lee, Wyeth BioPharma, Andover, Massachusetts, USA.
High-level expression of a difficult to express soluble receptor: Fc fusion protein in CHO cells.
11 :50 – 12 :20 AM James Piret, Biotechnology Laboratory, University of British Columbia,
Vancouver, British Columbia, The rush to high expression: Rock of Sisyphus or targeted
development? 12 :20 – 12: 40 PM Dirk-Jan Opstelten, Crucell NV, Leiden, Netherlands.
The human cell line PER.C6TM as an expression platform for the production of recombinant antibodies.
12 :40 – 2: 20 PM Lunch 2: 20 – 3: 50 PM Session 8: Protein Quality Chair: Martin Sinacore, Wyeth BioPharma, Andover, Massachussetts,
USA. 2: 20 – 2 :50 PM Rebecca McCoy, Amgen, Seattle, Washington, USA
Heterologous protein expression in mammalian cells can lead to protein misfolding that is unrelated to cell specific productivity.
2: 50 – 3 :20 PM Stefanie Weikert, Genentech, San Francisco, California, USA.
Modulation of effector functions by deleting the disulfide bonds in the hinge region of monoclonal antibodies.
3: 20 – 3: 50 PM Mike Butler University of Manitoba, Winnipeg, Manitoba, Canada. Culture parameters that cause perturbations in the glycosylation of
proteins secreted by mammalian cells. 3: 50 – 4: 15 PM Coffee Break 4: 15 – 6: 30 PM Industrial Workshops 7: 00 – 9: 00 PM GALA DINNER 9: 00 – 10: 30 PM Social Hour
Thursday, September 11, 2003 7 : 00 – 9: 00 AM Breakfast 9 : 00 – 9: 40 AM KEYNOTE LECTURE
Danial Wayner Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Ontario, Canada
9 : 40 – 11: 00 AM Session 9: High-Throughput Strategies Chair: Balwant Patel, Roche Applied Science, Indianapolis, IN, USA.
9 : 40 – 10: 10 AM Jennings Worley, Amphora Discovery Corp., Durham, NC, USA. Cell-based assays in the microfluidic environment.
10 :10 – 10: 40 AM Raymond Stevens, The Scripps Research Institute, La Jolla, CA, USA.
Design of high-throughput protein production methods for structural biology
10 :40 – 11: 10 AM Sumit Chanda, Genomics Institute of the Novartis Research
Foundation, San Diego, California, USA. Genomic-scale functional annotation through modulation of gene
expression. 11 :10 – 11: 30 AM CLOSING REMARKS
Amine Kamen, (BRI-NRC, Montréal, Canada). 4 :30 PM Site Visits (BRI and DSM Biologicals Montréal)
Poster Sessions P1 Baculovirus And Insect Cell Technology (9 posters) P1.1 Production of adeno-associated virus by baculovirus/insect cells
system in suspension cultures. Jamal Meghrous, Danielle Jacob, Parminder Chahal, Normand Arcand, Marc G. Aucoin, and Amine A. Kamen.
P1.2 A new method for the rapid and automated generation of baculovirus expression vectors.
R.B. Hitchman, R.D. Possee H. Irving and L.A. King. P1.3 The development of a new inducible plasmid-based expression cassette
for transient and stable recombinant protein production in insect cell lines.
K. S. Richards, C. M. Griffiths, S. Dales, L. A. King. P1.4 A novel method for the generation of influenza B virus particles using a
baculovirus gene delivery system. Sabine Nakowitsch, Lars Toellner, Christian Kittel, Andrej Egorov and Reingard Grabherr.
P1.5 293 F – A versatile suspension cell line which can be used for transfection or BacMam transduction. Janet H. Parham, Patrick Condreay, Tony Jurewicz, Lynette McMillan, Marie Iannone, Mark Bickett, and Tom Kost.
P1.6 Enhancement of baculoviral display via fusion to the VSV G membrane anchor. Kirsi Ojala, Johanna Koski, Wolfgang Ernst, Reingard Grabherr, Ian Jones and Christian Oker-Blom.
P1.7 High-throughput protein expression in insect cells. Johanna Wahlberg, Anette Danielsson, Ana Elorza, Eileen McCall, Ryan Hicks, Ian Hardern, Christine Dartsch, and Mark Abbott.
P1.8 Development of a high cell density culture process for stable non-viral expression of recombinant secreted alkaline phosphatase (SEAP) by Trichoplusia ni High FiveTM insect cells.
Cynthia Elias, Marie-Christine Locas, Jamal Meghrous and Amine Kamen P1.9 A Novel Bacmid for Tracking Baculoviral Infections. James R. Miller and D. Bruce Baldwin.
P2 Viral vectors (5 posters) P2.1 Large-scale production of retroviral vectors with suspension adapted
293GPG cells. Karim Ghani, Alain Garnier, Helene Coelho, Julia Transfiguracion, Pierre Trudel, Amine Kamen.
P2.2 Large-scale adeno-associated virus (AAV) production by triple-transfection in bioreactor.
St-Laurent G, Jacob D, Arcand N, Chahal P, Kamen A, and Durocher Y. P2.3 Preparation of murine anti-adenovirus fiber monoclonal antibodies
suitable for internalization of adenovirus in non permissive cells. D. Jung, M.-C. Chevrier, I. Châteauneuf, M. Drouin and M. Guérin. P2.4 Optimization of adenovirus production in perfusion cultures using an
ultrasonic retention device. O. Henry, M. Perrier, A. Kamen. P2.5 Improved sindbis viral expression systems for recombinant protein
production. T Nivitchanyong, YC Tsai, PS Fishman, MJ Betenbaugh, GA Oyler.
P2.6 Highly-sensitive method for detecting replication competent adenoviruses (RCAs) and development of a RCA free cell line. Luc Gagnon, Pierre-Alain Moisset, Claude Larose, Luc Péloquin, Maude Simoneau, Claire Guilbault, Bernard Massie2 and Pierre Jolicoeur.
P3 Transient Transfection (9 posters) P3.1 A simple and scalable gene transfer method: Calfection
Natalie Muller, Jeanette Lindell, Philippe Girard, Elisabeth Derow, Raymond Jacquet, Martin Jordan, Florian M. Wurm.
P3.2 Large-scale transient transfection of serum-free suspension-growing HEK293-EBNA1 cells: peptone additives improve cell growth and transfection efficiency.
Pham PL, Perret S, Doan HC, Cass B, St-Laurent G, Kamen A, Durocher Y. P3.3 Recombinant protein synthesis limitations following transient
transfection of HEK293-EBNA1 cells. Carpentier E, Cass B, Kamen A, Durocher Y. P3.4 Purification of recombinant proteins from mammalian cell culture using
a generic double affinity chromatography method. Cass B, Pham PL, Kamen A, Durocher Y. P3.5 Cell culture protein expression studies using the X-tremeGENE Ro1539
reagent from Roche. Mick Shaw, Ian Hampton, April Greene and Elke Lullau. P3.6 Improved performance of mammalian expression vectors in transient
transfections in 293E cells. Ying Zhu, Soujuan Wang, Wei Xia, John McClary, Patrick Jones, Susan Harvey, Jimmy Gu, Peter Bringmann, and Ronald Cobb.
P3.7 The secrets of transfection in serum-free suspension culture. Mario Henke, Thomas Cremer, Nicola Di Maiuta, Klaus Memmert, Sabine Geisse. P3.8 A novel high-level transient protein expression and purification system for
Spodoptera insect cells that facilitates automated screening. Kathryn Loomis, Keith Yaeger, Mark Mehler, and Robert Novy. P3.9 Recombinant Expression and Purification of G Protein-Coupled Receptors.
Youn-Ok Shin, John Hanson, Mirek Cygler, Amine Kamen, Michele Loewen and Yves Durocher.
P4 Cellular And Molecular Engineering (8 posters) P4.1 Evaluation of the Flp-In TRex system with the GPR41 and Histamine
H3 receptors. Elaine Murrison.
P4.2 The Significance of Intracellular [ATP] to Reovirus Production. A. Burgener, K. Coombs, and M. Butler.
P4.3 Long™R3IGF-I as a more potent alternative to insulin in serum-free culture of CHO cells: cell survival, receptor activation and second messenger pathways.
Yandell, C.A., Butler, I.P., Sheehan, A.J., Wade, B., Simula, A.P. and Goddard, C. P4.4 The 3’ Segment of the cDNA for a Ser/Thr Kinase Dictates its
Biosynthesis in Mammalian Cells. Wee Guan Lim, Bee Jen Tan, Wenyu Bu and Wei Duan. P4.5 Comparison of protein production from two mammalian cells, CHO and
HKB11. M.-S. Cho, H. Yee, C. Brown, M. Ray, and S. Chan. P4.6 Identification of expressions of ¦Â-casein promoter controlling human
t-PA mutant in fibroblasts transfected by deleting prolactin receptor genes.
Ji –Xian Deng, Zheng-Tian Yang , Wei Shen , Xiao-Jie Wu. P4.7 Functional genomics of hyperoxaluria by profiling activated transcription
factors. Grewal, Jasjit S. P4.8 Advances in media optimization: two automated approaches that
increase expression while reducing development time. Stacy Holdread, , Cindy Hunt, Perry Haaland, Bryce Chaney, Warren Porter, Mohammad Heidaran, Jon Wannlund (Presented by James Brooks)
P5 Antibodies and Other Secreted Proteins (7 posters) P5.1 A new approach to characterize antibody expression in CHO cells using
real time quantitative PCR. Wittische C, de Wit C., Shawley R., Cajal B., Zhan D., Shimoni Y., Taticek R., and Xu Y.
P5.2 Monoclonal antibody production: determination of appropriate system and media for production enhancement and decrease of overall production time.
K. Fritchman, C Tilsaghani, Tish Sumnall. P5.3 Therapeutic mAbs: expression strategies during development. Greg Dean, Diane Hatton and Ray Field. P5.4 Production of recombinant proteins in the seminal fluid of transgenic
pigs. Dan Lacroix. P5.5 VP22 fused to GFP or to the herpes simplex virus thymidine kinase
does not translocate between cells. Vincent Roy, Jian Qiao and Manuel Caruso. P5.6 Expression of recombinant antibodies using a tri-cistronic
expression system. M. Schuster, G. Waxenecker, G. Himmler and H. Loibner. P5.7 Medium Optimization of Hybridoma Culture. D. De Alwis, R.L. Dutton, J. Scharer, M. Moo-Young.
P6 Protein Quality (5 posters) P6.1 Addition of bisecting N-acetylglucosamine residues to monoclonal
antibodies in vitro by recombinant N-Acetylglycosaminyltransferase III for enhanced biological activity.
Yuan Zhi Zheng, Jason Hodoniczky, David C. James. P6.2 Comparative N-glycan profile of r-SeAP produced in baculovirus-
infected insect cells at various harvest times. Adrián Delgado-Bustos, Octavio T. Ramírez and Sandino Estrada-Mondaca. P6.3 Modification of N-terminal sequence enhances overexpression of
recombinant human acetyl-CoA carboxylase 2. Thomas S. Suhar, Rongqi Wang, Xiaojun Wang, Rebecca J. Gum, Ernst U. Frevert, Christine A. Collins, and Regina M. Reilly.
P6.4 Characterization of folding and secretion of a recombinant protein expressed in mammalian cells using fusion protein strategies.
L Huang and R Kelly. P6.5 Process control using the ProteinChip® technology: expression
optimization and product quality monitoring. V. Brenac. and P. Santambien.