12
Product Catalog 2016 Order & Inquiry Tel: +1 713 732 2181 Fax: +1 866 747 4781 E-mail: [email protected] Order & Inquiry Tel: +49 89 46148500 Fax: +49 89 461485022 E-mail: [email protected] A Company of Selleck Chemicals

A Company of Selleck Chemicals · can simplify and accelerate your experiments without requiring PCR product cutting with restriction enzymes allowing flexible options for restriction

  • Upload
    others

  • View
    2

  • Download
    0

Embed Size (px)

Citation preview

Product Catalog 2016

Order & InquiryTel: +1 713 732 2181 Fax: +1 866 747 4781E-mail: [email protected]

Order & InquiryTel: +49 89 46148500 Fax: +49 89 461485022E-mail: [email protected]

A Company of Selleck Chemicals

Cell to cDNA Kit.....................................................................................................................1-2

Quick-Fusion Cloning Kit..........................................................................................................3

All-in-One cDNA Synthesis SuperMix......................................................................................4

Bimake SYBR Green qPCR Master Mix..................................................................................5

2x SYBR Green qPCR Master Mix................................................................................. 6

2x SYBR Green qPCR Master Mix (Low ROX)...............................................................7

Protease Inhibitor Cocktail (EDTA-Free, 100x in DMSO)........................................................ 8

Protease Inhibitor Cocktail (EDTA-Free, mini-Tablet)..............................................................9

Phosphatase Inhibitor Cocktail (2 Tubes, 100x)...................................................................... 10

Anti-DYKDDDDK (Flag) Affinity Gel........................................................................................ 11

Protein A/G Magnetic Beads for IP..........................................................................................12

Magnetic Separator..................................................................................................................13

DNA Transfection Reagent.................................................................................................14-15

Annexin V-FITC Detection Kit.................................................................................................. 16

Cell Counting Kit-8 (CCK-8).....................................................................................................17

Mouse Direct PCR Kit (For Genotyping)..................................................................................18

Contents

1 2

Customer Reviews

Fig a: After decades of qPCR research, we were used to the TRIzol method for mRNA isolation. But things changed when Bimake Cell to cDNA kit was brought to us. It is much more efficient, reliable, and very impressive!

Cell to cDNA Kit(Bimake)

(Thermo Fisher Scientific)TRIzol Reagent

Cat #: B25601,B25602

Cell/Tissue to cDNA Kit (Magnetic Beads)

B25601B25602

25 Rxn 100 Rxn

USD 125USD 405

Cat.No. Size Price

DescriptionThe Cell to cDNA Kit is perfect for rapid and efficient purification of mRNA directly from cell cultures. It contains reverse transcription-related reagents to perform the first-strand cDNA synthesis. The kit utilizes macromolecule modified beads which enable high specific binding with mRNA. The resulting high-quality purified mRNA is free of DNA, proteins, and other kinds of RNAs. Thi kit does not contain toxic solvents. The simple Cell to cDNA kit protocol takes approximately one hour to obtain cDNA from cell cultures.

30min 60min

Magnetic Beads (Bimake) TRIzol (Thermo Fisher)

Advantages

Price Comparison

USD/rxn

Bimake Thermo

4.10

5.00

Magnetic Beads + RT TRIzol + RT

vs

2. Without toxic Reagents

Avoid using toxic Reagents, such as TRIzol.

1. Time saving

Only 30min needed for mRNA extraction and 10min for reverse transcription. No centrifugation necessary.

3 4

DescriptionThe Quick-Fusion Cloning Kit is designed for fast, directional cloning of DNA fragment into any vector at any site. It can simplify and accelerate your experiments without requiring PCR product cutting with restriction enzymes allowing flexible options for restriction enzyme sites when linearizing the vector. Additionally, the linearized vector and PCR product can be cyclized in only 30 minutes at 37°C. Compared to the conventional cloning method, the 15 base tract is added at the ends of PCR primers corresponding to the ends of the linearized vector.

1. Generate a linearized vector 2. PCR amplification of the insert

3. Set up the fusion cloning reaction

4. Transformation

RE digestion, PCR, ... Gene-specific primers with 15bpextension homologous to vector ends

Clone screening

Quick-Fusion Cloning KitIncubation at 37°C for 30 minutes

Flow Chart

Cat #: B22611, B22612

Quick-Fusion Cloning Kit

B22611B22612

40 rxns100 rxns

USD 123USD 249

Cat.No. Size Price

Description

All-in-One cDNA Synthesis SuperMix Cat #: B24403, B24408

B24403B24408

200 rxns1000 rxns

USD 300USD 1000

Cat.No. Size Price

All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR).

Price Comparison

Procuct FeatureRT-PCR

Qrt-PCR

Enzyme activity temperature

RT extension time

Maximal cDNA length

RNase H deficiency

Application

×

42℃

15-30 mins

≤ 12kb

cDNA Synthesis for real time-PCR assay

ALL components in ONE tubeJUST RNA template and START!

Reaction Buffer

1. Reverse Transcriptase2. RNase Inhibitor3. dNTPs4. Oligo dT/Random primer Mix

RNA template

Price Comparison

0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0

Clontech

GeneCopoeia

NEB

SBI

Bimake

Price:USD

12.0

13.0

14.0

2.5

USD/rxn

19.0

0 1.00 2.00 3.00 4.00 5.00 6.00

Bio-Rad

Thermo

Bimake

Price : USD USD/rxn

4.65

4.78

1.00

Promega 4.80

5 6

Cat #: B21202, B21203

2x SYBR Green qPCR Master Mix

Customer Reviews

B21202B21203

5 mL25 mL

USD 158USD 580

Cat.No. Size Price

Fig: Real-time quantification using SYBR green qPCR master mix from Bimake, Roche, Takara and Abi Biosystems, respectively. This data shows higher detection sensitivity of Bimake SYBR green qPCR master mix than that of others.

BimakeRoche

BimakeAbi

Sample 3Sample 2

Bimake

Takara

Bimake vs Roche Bimake vs Takara Bimake vs Abi

Sample 1

Bimake SYBR Green qPCR Master Mix

Fast Procedure Option Has Arrived for B2120x, B2170x

Upgrade to Procedure CompatibleFast

Buying Guides

Note: The previous B2180X have been replaced by upgraded B2120X

Bimake SYBR Green qPCR Master Mix Product Family:

Type

Basic Mix

Premix with ROX

Cat.No.

B2120X

B2170X

Advantage

With two kinds of ROX separately included

Premix low concentration ROX

0 200.00 400.00 600.00 800.00 1,000.00 1,200.00 1,400.00 1,600.00 1,800.00 2,000.00

Bimake

Price : USD Package Scale: 25 mL5 mL

Roche 1,050.00220.00

Bio-Rad 1,225.00275.00

580.00158.00

Life Tech (Abi) 1,534.00342.00

DOES NOT USEROX Reference Dye

Bio-Rad CFX96™, CFX384™, iCycleriQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Illumina Eco qPCR;Eppendorf Mastercycler® eprealplex, realplex 2s; Thermo Scientific PikoReal Cycler;QIAGEN / Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;Roche Applied Science LightCycler™ 480;

Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;StepOne™, StepOne Plus™.

Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™,MX3005P™, MX3000P™, QuantiStudio™ 6K/7K/12K Flex.

USE ROXReference Dye 1USE ROXReference Dye 2

Applied Real-time PCR Instrument

Price Comparison

Equipment ROX CatProcedure

Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT

Applied Biosystems, 7900HT Fast; StepOne™, StepOne Plus™.

Applied Biosystems 7500,

Applied Biosystems 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™, QuantiStudio™ 12K Flex.

Opticon®, Opticon 2, Chromo4™; MiniOpticon™, Cepheid SmartCycler®

Bio-Rad CFX96™, CFX384™, iCycleriQ™, iQ™ 5, MyiQ™,; Eppendorf Mastercycler® eprealplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCycler™ 480; Thermo Scientific PikoReal Cycler.

USE High ROX

USE Low ROX

Do NOT USE ROX

B2120X

B2120XB2170X

B2120X

Standard 2-step

3-step

Fast 2-step

X

X

X

0 100.00 200.00 300.00 400.00 500.00 600.00 700.00

483.00

618.00

450.00

270.00

79.00

68.00

63.00

37.00

Merck-Calbiochem

Pierce

Sigma

Bimake

Price : USD Package Scale: 10 mL (100x)1 mL (100x)

456.0084.00Roche

7 8

Bimake SYBR Green Mix Bimake SYBR Green Mix with ROX

Mix Manually

+

ROX Master Mix ROX Premixed Master Mix

More Convenient

VS

Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™,MX3005P™, MX3000P™, QuantiStudio™ 6K/7K/12K Flex.Low ROX (Cat # : B21702, B21703)

● Low ROX are provided for the following qPCR instruments:

2x SYBR Green qPCR Master Mix (Low ROX) Cat #: B21702, B21703

Price Comparison

PropertiesIngredient

AEBSF

Aprotinin

Bestatin

E-64

Leupeptin

Pepstatin A

Targets

Serine proteases

Serine proteases

Aminopeptidases

Cysteine proteases

Serine and cysteine proteases

Aspartic proteases

Protease Inhibitor Cocktail - EDTA-Free, 100x in DMSO Cat #: B14001, B14002

Customer Reviews

Cell Culture: D-MEM with 10% FBS.Lysis buffer: RIPA. Protease inhibitor cocktail: Bimake PI. Bimake Protease Inhibitor Cocktail provides protein stabilization for your protein of interest. Low-expression of protein in HCT-116 cells can also be detected clearly.

Cell LineProtein

of interest

α-Tubulin

HCT-116

Cell Line: U87MG.Lane1: PTBP2 shRNA-control.Lane2: PTBP2 shRNA-1#.Lane3: PTBP2 shRNA-2#.Bimake PI: Bimake PI cocktail in liquid form.Roche PI: Roche PI cocktail in tablet.We switched to Bimake protease inhibitors cocktail.Because it has a popular price and same effect.

--: M-PER lysis buffer without protease inhibitors.

Cell Line: MCF7.Condition: 2 hours at 37℃.

Bimake PI: Bimake PI cocktail in liquid form, with M-PER. Roche: PI cocktail in tablet, Sigma: PI cocktail in liquid, PMSF: 1 mM, all with M-PER.Bimake cocktail provides the best protection for PARP, leaving competitors behind.

PARPPI

Time

p27

-- -- Bimake Roche Sigma PMSF 0 h 37℃, 2 h

B14001B14002

1 mL1 mL x 10

USD 37USD 270

Cat.No. Size Price

Roche PI

PTBP1β-actin

PTBP1β-actin

Bimake PI

B21702B21703

5 mL25 mL

USD 158USD 580

Cat.No. Size Price

0 200.00 400.00 600.00 800.00 1,000.00 1,200.00 1,400.00 1,600.00 1,800.00 2,000.00

Bimake

Price : USD Package Scale: 25 mL5 mL

Roche 1,050.00220.00

Bio-Rad 1,585.00366.00

580.00158.00

Life Tech (Abi) 1,798.00423.00

Price Comparison

100.00 200.00 300.00 400.00 500.00 600.00 700.00 800.00 900.00 1,000.000

730.00107.00Pierce

444.0065.00Sigma

900.00150.00Roche

Price : USD Package Scale: 10 mL (100x)1 mL (100x)

120.00Merck-Calbiochem 936.00

Bimake 37.00 270.00

9 10

Cat #: B15001, B15002

Phosphatase Inhibitor Cocktail - 2 Tubes, 100x

Customer Reviews

Ingredient

Sodium Fluoride

Sodium Orthovanadate

Sodium Tartrate

Sodium Molybdate

Imidazole

(-)-p-Bromotetramisole oxalate

Cantharidin

Microcystin LR, Microcystis aeruginosa

Tube #

A (Aqueous)

B (DMSO)

Targets

Acid phosphatases

Alkaline phosphatases, PTPs, ATPases

Acid phosphatases

Acid and phosphoprotein phosphatases

Alkaline phosphatases

Alkaline phosphatases

Ser/Thr phosphatases

PP1 and PP2A

--0 1 3 5 7

Roche

0 1 3 5 7Bimake

0 1 3 5 7p-Akt (S473)

Tubulin

Time (h)

Cell Line: 293TCondition: 0, 1, 3, 5, 7 hours at 25℃.--: EBC lysis buffer only.Roche: EBC with PI (Roche) and PPI (Roche).Bimake: EBC with PI (Roche) and PPI (Bimake).

Time

C4 Roche BimakeRoche Roche Roche Roche

-- ---- Roche

C3

4℃, 16 h

PIPPI

p-Akt

Tubulin-1

Cell Line: 293Condition: 16 hours at 4℃.Bimake: Bimake PPI in 2 tubes.Roche: Roche PPI tablet.C3 & C4: PPI from competitor #3 and #4. Bimake allows for detectable phosphorylated-Akt for 16-hour incubation at 4℃ in 293 cells.

Bimake C3 C4 PIERCE RocheRoche-- -- --PIERCE BimakeRoche Roche-- -- Roche

0 h

Roche Roche

RT, 2 hTime

PIPPI

p-Akt

Tubulin-1

Cell Line: HeLa Condition: 0, 2 hours at 25℃.Bimake: Bimake PPI in 2 tubes.Roche: Roche PPI tablet.C3 & C4: PI & PPI from competitor #3 and #4.Bimake inhibitor cocktails give the best performance!

Cat.No. Size PriceB15001B15002

1 mL A + 1 mL B(1 mL A + 1 mL B) x 10

USD 37USD 270

Price Comparison

Price Comparison

Properties

Cat #: B14011, B14012

Protease Inhibitor Cocktail - EDTA-Free, mini-Tablet

Customer Reviews

B14011B14012

10 pcs100 pcs

USD 42USD 297

Cat.No. Size Price

CHO cell lysate was treated in 37℃ for 2h, different ratios of cocktail are added.

With the help of Bimake mini-Tablet Protease Inhibitor Cocktail, we demonstrated clear RNAi activity in our results.

Bimake mini-Tablet Protease Inhibitor Cocktail is compatible with DAT co-IP experiments.

AdvantageAccurate IngredientsClearance of components and concentrations help you to avoid uncertainty.

Comprehensive ProtectionProvides protection for all kind of proteins, especially for large ones.

Lower costsPrice is less than half of competitors’.

Accurate IngredientsComprehensive

Protection

Lower costs

297.00

0 50.00 100.00 150.00 200.00 250.00 300.00 350.00 400.00 450.00 500.00

Roche

Bimake

Price : USD Package Scale: 100 Tablets10 Tablets

42.00

84.00 456.00

1,360.00

0 200.00 400.00 600.00 800.00 1,000.00 1,200.00 1,400.00 1,600.00

Sigma

Price : USD Package Scale: 5 mL1 mL

480.50

799.00Bimake 249.00

11 12

Cat #: B23201, B23202

Protein A/G Magnetic Beads for IP

Customer Reviews

Immunoprecipitation for HSA: 100 μg of Bimake Protein A/G Magnetic beads & 10 μg Anti-HSA Antibody was used

Heavy Chain

1 2

50

35

20

HSA

Light Chain

50

35

20

Immunoprecipitation for GST-Tagged Fusion Protein: 100 μg of Bimake Protein A/G Magnetic beads & 10 μg Anti- GST-Tag Antibody was used)

GST-Tagged Fusion Protein

1 2

Heavy Chain

Light Chain

Immunoprecipitation for GST-Tagged Fusion Protein: 1. Antigen & Anti-GST-Tag Antibody (Mouse IgG1)2 & 3. Elution after IP4. Antigen & Anti-GST-Tag Antibody (Mouse IgG1)5 & 6. Elution after IP (Control group without GST-Tag Fusion Protein)

50

35

20

M 1 2 3 4 5 6

GST-Tagged Fusion ProteinHeavy Chain

Light Chain

Much Faster

IP Finished

Start Experiment

Bimake Protein A/G Magnetic Beads

30 minProtein A/G Agarose Gel

120 min

1

2

1 2

Immunoprecipitation for HSA:1. 5 μg of rabbit polyclonal antibody was used2. 5 μg of mouse IgG1 monoclonal antibody was used

Heavy ChainHSA

Light Chain

50

35

20

85

B23201B23202

1 mL5 mL

USD 73USD 306

Cat.No. Size Price

Price Comparison

Cat #: B23101, B23102

Anti-DYKDDDDK (Flag) Affinity Gel

DescriptionAnti-Flag Affinity Gel is a Protein A purified murine IgG2b Monoclonal antibody covalently attached to sepharose 4B gel.

Customer Reviews1 2Bimake

1 2

200200

11697

66

45

31

21

14

11697

66

45

31

21

14

Sigma M2

1. 1% Input2. Bimake Gel3. Bimake Gel + IP4. Sigma M2 Gel + IP

Protein-FLAG (85 kD)

1 2 3 4

Cell line:HEK293T

1. 2% Input 2. Bimake Gel 3. Bimake Gel + IP 4. Sigma M2 Gel + IP Protein-FLAG (40 kD)

1 2 3 4

Cell line: HEK293T

1. Sigma M2 Gel 2. Sigma M2 + IP 3. Bimake Gel4. Bimake Gel + IP

1 2 3 4

Protein-FLAG (90 kD)

Cell line: HEK293T

Cell line: Protein-FLAG (90 kD) overexpressed HEK293T1. ANTI-FLAG Affinity Gel + IP (Protein-FLAG (90 kD) overexpressed HEK293T)2. ANTI-FLAG Affinity Gel + IP (HEK293T)

B23101B23102

1 mL5 mL

USD 249USD 799

Cat.No. Size Price

0 100.00 200.00 300.00 400.00 500.00 600.00 700.00

Price : USD Package Scale: 5 mL1 mL

306.0073.00Bimake (Magnetic Beads)

300.0060.00Santa Cruz (Agarose)

170.00Life Tech (Magnetic Beads) 670.00

Price Comparison

PropertiesConcentration

Particle size

Binding capacity of human IgG

Ligand

Application

Recommended volume

pH stability

Shipped in

10 mg/mL

2 μm

0.4-0.5 mg/mL

Recombinant protein A/G

rProtein Purification, Immunoprecipitation

IP: 5 μL gel for 500 μL crude protein solution

6-8 (Long term)

Blue ice

PropertiesAntibody clone

Antibody isotype

Antibody purification

Antibody bound

Application

Recommended volume

Binding capacity

Shipped in

1E6

Mouse IgG2b

Protein A purified

NLT 7.5 grams antibody / Liter gel

Protein Purification, Immunoprecipitation

IP: 5 μL gel for 500 μL crude protein solution

Minimum 1.1 mg of a Flag fusion protein eluted per mL of packed resin

Blue ice

Met-N-terminal FLAG fusion protein: Met-FLAG–ProteinN-terminal FLAG fusion protein: FLAG–ProteinC-terminal FLAG fusion protein: Protein-FLAG

Binding characteristics

13 14

Cat #: B35101, B35102

DNA Transfection Reagent

Customer Reviews

Description

B35101B35102

1.5 mL1.5 mL × 10

USD 149USD 980

Cat.No. Size Price

The Bimake DNA Transfection Reagent is a novel nano-material with low cytotoxic formulations for efficient transfection of a broad range of eukaryotic cells. Experimental uses include cellular analysis and protein expres-sion.

Cat #: B23803

Magnetic Separator

DescriptionThis Magnetic Separator is a proprietary machine, supporting the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time. This product is suitable for antibody purification, immunoprecipitation, co-immunoprecipitation, cell sorting and nucleic acid separation.

ApplicationMagnetic Separator (2 mL/200 μL /15mLseparator)is suitable for magnetic separation test experiments, which use general 1.5mL EP tube, 2mL EP tube, 200ul PCR tube and 15mL centrifuge tube.

B23803 2 mL/200 uL USD 150Cat.No. Size Price

0 1,000 2,000 3,000 4,000 5,000 6,000

6,117.00748.50FuGene® HD

4,450.00528.00Lipofectamine® 2000

980.00149.00Bimake

Package Scale: 1.5 mL × 101.5 mLPrice : USD

7,000

Advantages

CostEffective

LowCytotoxicity

HighEfficiency

High Efficiency High transfection efficiencies in a broad range of eukaryotic cells.

Low Cytotoxicity Non-liposomal transfection reagent with low cytotoxicity.

Cost Effective Offered at one-fourth the price as compared to Lipofectamine® 2000.

Price Comparison

Price Comparison

0 100.00 200.00 300.00 400.00 500.00 600.00 700.00 800.00 900.00

Life Technologies

Bimake

Package Scale: 2 mL/15 mLPrice : USD

546.00

60.00

15 16

Low Cytotoxicity Phase contrast microscopy of HeLa cells 48 h after transfection.

Bimake Lipofectamine® 2000

Customer Reviews

DescriptionAnnexin V-FITC kit provides a rapid and convenient assay for apoptosis. Annexin V-FITC kit detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green- fluorescent fluorescein isothiocyante (FITC) dye and necrotic cells using propidium iodide (PI). These can be detected using flow cytometry or fluorescence microscopy.

Fig b. Fluorescence microscope analysis of Jurkat cells treated with 8 ng/mL TNF for 24 hours, detected by Annexin V-FITC kit.

Fig a. Cell apoptosis of tumors inoculated in Hyperbaric oxygen (HBO)and control groups, analyzed using flow cytometry.

DAPI PI DAPI&PI

Annexin V PI&Annexin V DAPI&Annexin V

Cat #: B32115, B32117

Annexin V-FITC Detection Kit

B32115B32117

50 rxns100 rxns

USD 149USD 249

Cat.No. Size Price

0 2.00 4.00 6.00 8.00 10.00 12.00 14.00

Abcam

Millipore

Bimake

Price : USD USD/rxn

11.57

5.78

2.49

BD 6.25

Price Comparison

17 18

Customer Reviews

55℃, 30 min

Tissue Digestion Protease Inactivation

95℃, 5 min

2 x M-PCR Mix

PCR

Genotyping of Gene X knockout mice Genotyping of Gene Y knockout mice Direct PCR results with different primers

Mouse Direct PCR Kit - For Genotyping Cat #: B40013, B40015

Description The Mouse Direct PCR Kit provides a fast preparation and PCR amplification that is specifically designed for mouse genotyping. 2 x M-PCR Mix (which includes an optimized Taq Polymerase and loading dye) ensures high amplifica-tion efficiency of target DNA.

Genotyping R

esult Obtained

Much Faster

Proteinase K (DNA Purification)

>15h

NaOH-HCl (DNA Purification)

>4h

Bimake

Mouse Direct PCR Kit

2.5h1

2

3

B40013B40015

200 rxns500 rxns

USD 127USD 267

Cat.No. Size Price

Add 10% (v/v) Cell Counting Kit-8 (CCK-8) Culture for 0.5-4 hours Record OD450nm

Customer Reviews

Description

Brief Summary of the Protocol

The Bimake Cell Counting Kit-8 (CCK-8) is a ready-to-use one-bottle solution which offers a simple, rapid, reliable and sensitive measurement of cell viability and cytotoxicity of various chemicals.. The detection sensitivity of Cell Counting Kit-8 (CCK-8) is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Cat #: B34302, B34304

Cell Counting Kit-8 (CCK-8)

B34302B34304

5 mL (500 rxns)25 mL (2500 rxns)

USD 63USD 180

Cat.No. Size Price

Fig. Different amount of cells were seeded into 96-well plate at different concentrations in 100 μl culture medium in triplicate. After adherence, 10 μl/well Bimake Cell Counting Kit-8 (CCK-8) or Dojindo CCK-8 was added, allowing cells to continuously culture for indicated duration in cell culture incubator. Absorbance at 450 nm was then measured in a microplate reader. This data demonstrates comparable performance of Bimake Cell Counting Kit-8 (CCK-8) and Dojindo CCK-8.

Price Comparison

Price Comparison

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

Bimake

Price : USD

0.1380

Sigma 0.64570.5368

Dojindo 0.20200.1240

0.0499

Package Scale: USD/rxn (500 rxns)USD/rxn (Max Size)

0 0.20 0.40 0.60 0.80 1.00 1.20 1.40

Price : USD USD/rxn

Lamda Biotech 1.25

Kapa Biosystems 0.76

Clontech 0.64

Thermo Scienti�c 0.63

Bimake 0.39