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BiochemistryProgrammeSchoolofLifeSciences

TheChineseUniversityofHongKong

This document tries to give our students an overview of the BiochemistryProgramme in theSchoolofLifeSciences (SLS)atCUHK. It iscomposedof (A)Background of the Biochemistry Programme, (B) Suggested Study Streams forcareerdevelopmentand(C)CourseListandCourseOutline(2016-2017version).After finishingthecourses in theProgramme,youshouldbeable toappreciatehowlifefunctions.Also,itisimportanttoplanaheadtomapoutyourcareerpath.HopeyouenjoyyourstudiesintheBiochemistryProgrammeattheCUHK.

(A)BackgroundoftheBiochemistryProgramme

Biochemistry is a branch of science that investigates the chemical compoundsand processes occurring in living organisms at molecular level. Theknowledge procured from the study in Biochemistry has found extensiveapplicationsinmedicineandbiotechnologythatdrasticallyrevolutionizeourdailylife.

Ourprogrammeaimsto1) provide concepts andmechanisms of biochemical processes,with emphasisonclinicalandbiomedicalsciences;

2) providetrainingonthelatestbiochemicaltechnology;3) cultivate the abilityof critical thinking, aproactive and responsible attitudeandefficient communication skills forhigh competitiveness in further studyandcareerdevelopment.

History:Biochemistry Department was established in 1971, when the first batch ofM.Phil. students was admitted. Prof. Lin MA, the second Vice-President ofCUHK,wastheDepartmentHead.In1973,thefirstbatchofM.Phil.studentsgraduated and the Department had admitted the first batch of majorundergraduate students via transferring from other departments as UnitedCollege students. This first batch of undergraduates graduated in1976.BiochemistryDepartmentisalsoafoundingDepartmentoftheMedicalSchool at CUHK. In 2010, Biochemistry Department merged with BiologyDepartmenttoformtheSchoolofLifeSciencesundertheFacultyofScience.

Highlight/StudyAreas(Formoredetails,pleaserefertoPart(C):Our curriculum emphasizes on current topics in biochemistry and molecularbiology that have scientific, medical and social significance. Major studyfocusesincludegeneticsandcellbiology,proteinandenzymes,bioenergeticsand metabolism, methods in biochemistry and molecular biology, andbiomedical and health sciences. We also provide a wide range of elective

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courses for students to attain professional knowledge in specializeddisciplines such as clinical biochemistry, immunology, endocrinology,neuroscience,forensicsciencesandsportsciences,etc.Ourcurriculumisalsodesigned to provide experiential learning through self-study modules,laboratory practicals and independent research. Apart from conventionteachinginlecturetheatersandstudentlaboratory,ourprogrammeprovidesa number of eLearning materials/topics and they can be found in thefollowingwebsite‘http://www.bch.cuhk.edu.hk/learnbiochem/’.

CareerProspects(Formoredetails,pleaseseePart(B):• Nearlyhalfofourgraduatespursuepostgraduatestudiesinlocaloroverseasuniversities.

• About one fifth of our graduates have joined the medical and researchlaboratories.

• Some other graduates have entered the education, commercials, industrialandgovernmentsectors

ContactInformationWebsite:www.bch.cuhk.edu.hkEmail:biochemistry@cuhk.edu.hkTel:(852) 3943 6359

ProgrammeDirector:ProfessorSHAW Pang Chuipcshaw@cuhk.edu.hk

ProgrammeSecretary:Ms.JannyLeejanny@cuhk.edu.hk

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(B)SuggestedStudyStreamsforCareerDevelopment

CourseListofBiochemistryProgramme(BCHECoded)CourseCode CourseTitle Unit(s)BCHE2000 FrontiersinBiochemistry 2BCHE2030 FundamentalsofBiochemistry 3BCHE2070 ResearchInternship 2BCHE3030 MethodsinBiochemistry 3BCHE3040 ProteinsandEnzymes 3BCHE3050 MolecularBiology 2BCHE3070 RecombinantDNATechniques 1BCHE3080 BioenergeticsandMetabolism 3BCHE3090 Self-studyModulesinBiochemistry 2BCHE3650 MolecularBiologyandRecombinantDNALaboratory 2BCHE3730 AnalyticalBiochemistryLaboratory 2BCHE4030 ClinicalBiochemistry 3BCHE4040 AspectsofNeuroscience 3BCHE4060 BasicandAppliedImmunology 3BCHE4070 Management and Accreditation of Biochemical

Laboratory3

BCHE4080 BiochemistryforForensicSciences 2BCHE4090 BiochemistryforSportandExercise 2BCHE4130 MolecularEndocrinology 3BCHE4760 ImmunologyandHaematologyLaboratory 2BCHE4830 MedicalBiochemistryLaboratory 2BCHE4901 SeniorExperimentalProjectI 2BCHE4902 SeniorExperimentalProjectII 2BCHE4903 SeniorExperimentalProjectIII 2

B1)SuggestedstudystreamwiththecoursesinSLSfordifferenttypesofcareer/higherdegreestudies

MedicalBiochemistryStreamBCHE4030 ClinicalBiochemistryBCHE4060 BasicandAppliedImmunologyBCHE4070 ManagementandAccreditationofBiochemicalLaboratoryBCHE4130 MolecularEndocrinologyBCHE4760 ImmunologyandHaematologyLaboratoryBCHE4830 MedicalBiochemistryLaboratoryBIOL3410 GeneralMicrobiologyBIOL3630 AnimalPhysiologyBIOL4310 HumanGeneticsFNSC4101&FNSC4102HumanPhysiologyforNutritionStudiesI&II

EnvironmentalScienceStreamBIOL2210 EcologyBIOL4220 EnvironmentalBiotechnologyBIOL4260 ConservationBiology

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ENSC2270 IntroductiontoEnvironmentalScienceENSC3520 EnvironmentalandBiochemicalToxicologyENSC4250 EnvironmentalHealth

FoodandNutritionalScienceStreamBCHE4090 BiochemistryforSportandExerciseFNSC2002 NutritionforHealthFNSC3010 NutritionandHumanDevelopmentFNSC3030 NutritionalBiochemistryFSNC3180 FoodMicrobiologyFNSC4150 IntroductiontoMedicalNutritionTherapyFNSC4101&FNSC4102HumanPhysiologyforNutritionStudiesI&II

B2)SuggestedStreamsthroughOtherCourses(The proposed courses offered by other departments can be used to fulfill their minorprogrammes.Pleaseseetheirprogrammerequirements.).

BiochemicalEngineeringBMEG2001 IntroductiontoBiomedicalEngineeringBMEG2011 BiomedicalEngineeringLaboratoryBMEG3102 BioinformaticsBMEG3430 BiomaterialsandTissueEngineeringBMEG4450 BionanotechnologyBMEG4510 BiomolecularEngineeringBMEG4520 CardiovascularEngineeringBMEG4530 MusculoskeletalTissueEngineeringBMEG4540 Electrophysiology

ChemistryStreamBCHE4080 BiochemistryforForensicSciencesCHEM2120 MainGroupChemistryCHEM2200 OrganicFunctionalGroups:StructureandReactivityCHEM2300 ThermodynamicsandChemicalEquilibriumCHEM2400 AnalyticalChemistryCHEM2408 AnalyticalChemistryLaboratoryICHEM2820 OrganicChemistryLaboratoryICHEM2830 PhysicalChemistryLaboratoryICHEM3410 InstrumentalAnalysisCHEM4430 AccreditationofLaboratoryTestsCHEM4788 ChemicalApplicationsinForensicScience

BusinessAdministrationBCHE2070 ResearchInternshipBCHE4070 ManagementandAccreditationofBiochemicalLaboratoryMBTE3000 BusinessandSocialAspectsofBiotechnologyMGNT1010 IntroductiontoBusinessMGNT1020 PrinciplesofManagementDSME1035 FundamentalsofBusinessEconomics

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MGNT2510 IntroductiontoInternationalBusinessFINA3010 FinancialMarketsMKTG3030 IntegratedMarketingCommunication

EducationStreamEDUC2120 PrinciplesandImplementationofCurriculumandInstructionalDesignEDUC2210 EducationandSocietyinHongKongEDUC2240 UnderstandingSchoolingandEducationPolicyinHongKongEDUC2312 ChildandAdolescentDevelopmentEDUC3260 TeacherDevelopmentandLeadershipEDUC3311 PsychologyAppliedtoLearningandTeachingEDUC4130 InformationTechnologyinEducation

GeographyStreamGRMD1402 GlobalChangeandEnvironmentalSustainabilityGRMD1404 GeographicalLandscapeoftheWorldGRMD2209 PhysicalGeologyGRMD2221 WeatherandClimateGRMD3203 UrbanEnvironmentalProblemsGRMD3404 NaturalHazardsandHumanResponses

ManagementMGNT1010 IntroductiontoBusinessMGNT1020 PrinciplesofManagementMGNT2040 HumanResourceManagementMGNT3010 OrganizationalBehaviourMGNT4080 ManagerialSkillsforModernManagersMGNT4090 TechnologyandInnovationManagement

PsychologyStreamPSYC1000 GeneralPsychologyPSYC2010 IntroductiontoStatisticsPSYC2190 PhysiologicalPsychologyPSYC2780 NeuropsychologyPSYC3004 SpecialTopicsinClinicalandHealthPsychologyPSYC3720 HealthPsychology

SportScienceStreamBCHE4090 BiochemistryforSportandExercisePHPC2007 NutritionandHealthPHPC2016 TheoriesandConceptsofHealthBehavioursSPED2520 FunctionalHumanAnatomyandSportsInjuriesSPED2540 IntroductiontoExercisePhysiologySPED3550 PhysiologyofHumanPerformanceSPED3820 IntroductiontoExerciseandSportsPsychologySPED4560 PhysicalFitnessAppraisalandExercisePrescription

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(C)CourseListandCourseOutline

BCHE2000FrontiersinBiochemistry(2016-17,Term2)

DescriptionThiscoursepresentsthelatestdevelopmentsandadvancementsinbiochemistryandmolecularbiology.Itaimstoalertstudentstothetrendsandrecentbreakthroughsinbiochemicalandbiomedicalresearch.Contentswillvaryfromyeartoyear.

LearningOutcomeStudentsareexpectedtounderstandrecentdevelopmentsinresearchfieldsrelatedtoBiochemistryandMolecularBiology.Basicandnovelconceptsofvarioustopicsandtheirfuturedevelopmentswillbeintroducedandelaboratedbyteachers.Studentsareexpectedtoreadtherecommendedreadingsprovidedforeachlecturetopic,andtosearchforadditionalinformationinthelibraryandonlinetostudyontheirown.Towardstheendofthecourse,studentsarerequiredtopresentanideaoftheirinterestinaposterformat.

CourseSyllabusLecturetopicsaredeterminedbyindividualteachingstaffsofthecourse.Theyvaryeveryyeartoreflectthelatestdevelopmentinthefieldofbiochemicalresearch.Thelecturetopicscanbebrieflyclassifiedintothreemajorareas:NewResearchAreas(e.g.syntheticbiology),CellularBiochemistry(e.g.stemcells),andBiochemistryofDiseases(e.g.cancer,Alzheimer).

RequiredReadingsLecture handout for each of the topics will be uploaded to the accompaniedBlackboardsiteaweekbeforethelecture.Requiredreadingsforeachtopicwillbeprovided by the teaching staff concerned. The readingmaterials are expected tochangeeveryyeartoreflectthelatestdevelopmentintheresearchfield.

RecommendedReadingsAdditionalreadingmaterialswillbeprovidedbyindividualteachers.StudentsarealsoencouragedtousetheonlinePubMedsearchengine(http://pubmed.gov)tofindrelatedreferencematerialsforself-study.

EnrollmentRequirementsPre-requisite(s):BCHE2030

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BCHE2000FrontiersinBiochemistry(2016-17,2ndTerm)Wed9:30am–11:15am(W2-3);L1ScienceCentre

CLASSSCHEDULEWk.

Date Topicno.

Topic Teacher

1 Jan.11 1 Biochemistry:Past,PresentandFuture

PCS

2 Jan.18 2 SyntheticLife KMC3 Jan.25 3 RNABiology CKN4 Feb.1 - LunarNewYearVacation -5 Feb.8 4 Non-codingRNAinDevelopment HLH6 Feb.15 5 StemCellResearch SYT7 Feb.22 - GeneralDiscussion(Topics1-5) PCS/KMC/CKN/HLH/SYT8 Mar.1 Mid-termExamination(Topics1,3-

5)-

9 Mar.8 6 EtiologyofCancer SKK10 Mar.15 7 CancerTreatment WPF11 Mar.22 8 ProteinFoldingDisease KBW12 Mar.29 9 MetabolicDisease HKN13 Apr.5 10 BiochemistryofInfections FHL14 Apr.12 11 StructuralBiologyandDrug

DiscoveryWNA

15 Apr.19 - GeneralDiscussion(Topics6-11) SKK/WPF/KBW/HKN/FHL/WNA

TEACHERS’INFORMATIONTeacherName Office Tel. Email

Prof.ShannonAU(Coursecoordinator)

WNA SC178 39434170 shannon-au@cuhk.edu.hk

Prof.KMCHAN KMC SC184 39434420 kingchan@cuhk.edu.hkProf.WPFONG WPF MMW608 39436868 wpfong@cuhk.edu.hkProf.JeromeHLHUI HLH MMW503 39436316 jeromehui@cuhk.edu.hkProf.SKKONG SKK MMW609 39436799 skkong@cuhk.edu.hkDr.FHLO FHL MMW507B 39435019 lofaihang@cuhk.edu.hkDr.PatrickHKNgai HKN MMW507B 39434359 hkngai@cuhk.edu.hkProf.JackyCKNGO CKN SCEE403 39436346 jackyngo@cuhk.edu.hkProf.PCSHAW PCS SC180 39431363 pcshaw@cuhk.edu.hkProf.FayeSYTSANG SYT MMW607 39431020 fayetsang@cuhk.edu.hkProf.KBWONG KBW SC289 39438024 kbwong@cuhk.edu.hk

ASSESSMENTSCHEME4.55%(5marks) On-lineexercise:Topic19.1%(10marks) Groupposterpresentation:Topic231.85%(35marks) Mid-termexam(MCQs+shortQs):coveringTopics:1,3-554.5%(60marks) Finalexam(MCQs+shortQs):coveringTopics6-11

REFERENCESReadingmaterialswillbegivenbyindividualteachers.

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BCHE2030FundamentalsofBiochemistry(2016-17,Term1)

1. DescriptionThecourseintroducesthefundamentalprinciplesofbiochemistrythatareneededforalllifesciencedisciplines.Itwillcovertheimportanceofwater,structure-functionrelationshipsofbiomolecules(includingaminoacids,proteins,carbohydrates,lipidsandnucleicacids),thebiochemicallogicofthemetabolicpathways,andanoverviewofmetabolismwithemphasisonhowbiomoleculesareinterconvertingwitheachother.

2. Contents/FundamentalConceptsTopic Contents/FundamentalConcepts

Water – the mediumoflife

MolecularstructureofwaterWeakinteractionsinaqueoussystemAcid/baseequilibriumandbuffers

Carbohydrates

MonosaccharidesDisaccharidesandoligosaccharidesPolysaccharidesGlycoconjugatesBiologicalinformationandthesugarcode

LipidsStoragelipidsMembranelipidsOtherlipids

Nucleotide andnucleicacids

NucleotideNucleicacidsOtherfunctionsofnucleotides

Proteins

Aminoacid–basicbuildingblockofproteinsPeptidebondPrimary,secondary,tertiaryandquaternaryproteinstructuresHemoglobinasanexampletoillustratehowproteinsfunction

Thermodynamics – topredict if a reactionwillproceed

Freeenergyasameasureofareaction'stendencytoproceedspontaneouslyConceptsof'high-energy'compoundsandcoupledreactions

EnergyandMatters

Flowofenergyandcyclingofcarbon/nitrogeninthebiosphereEnergyrelationshipbetweencatabolicandanabolicpathwayOxidationofcarbonfuelsasamajorsourceofcellularenergyNAD(P)H/FADH2asuniversalelectroncarriersATPissynthesizedbyprotongradientsacrossmembranegeneratedbytransferringelectronstooxygenThelightreactionofphotosynthesisgeneratesbothprotongradientsandreducingequivalentsThedarkreactionofphotosynthesisusesthereducingpowergeneratedforbiosynthesisofsugarfromCO2

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BCHE2030FundamentalsofBiochemistry(2016-17,Term1)

Chemicallogicofbiochemicalreactions–topredicthowareactionshouldproceed

ElectronegativityandpolarityofcovalentbondSixdifferenttypesofbiochemicalreactions

Enzymecatalysis–topredicthowfastareactioncouldproceed

EnzymesaffectreactionratesbutnotequilibriaEnzymesincreasereactionratesbyloweringtheactivationfreeenergyConceptsoftransition-statecomplementarityWhybiochemicalreactionisstereospecific?BasicenzymekineticsEnzymesaregoodtargetfordrugdesign

Glycolysis Reactionsintheglycolyticpathwayareusedasexamplestoillustratetherecurringmotifsinmetabolicpathway

Vitamins Vitaminsareoftenco-factorsinenzymecatalysis

Overviewsofmetabolism

ConvergentcatabolismDivergentanabolismCyclicpathwayRegulatorystrategyCompartmentationofmetabolicpathways

3. LearningOutcomesToappreciatetheimportanceofwaterinlivingorganism.Tounderstandthestructure-functionrelationshipsofdifferenttypesofbiomolecules.Tounderstandthechemicallogic(thermodynamics,mechanism,andcatalysis)behindbiochemicalreactions.Tohaveanoverviewofmetabolicpathways,andappreciatehowbiomoleculesareinterconvertingwitheachother.

4. AssessmentSchemeMid-termexam. 30%Finalexam. 60%Assignment 10%

5. TextbooksNelson&Cox,LehningerPrinciplesofBiochemistry,W.H.FreemanBerg,Tymoczko&Stryer,Biochemistry,W.H.Freeman

6. LecturersProf.KBWong(courseco-ordinator),SC289tel:39438024email:kbwong@cuhk.edu.hkProf.KFLau,SC291tel:39431106email:kflau@cuhk.edu.hk

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BCHE2030FundamentalsofBiochemistry(2016-17,Term1)

7. CourseScheduleFriday8:30am–11:15am(F1-3);LeeShauKeeBuilding(LSKLT6)

Wk. Date Topic Teacher1 Sept.9 Introduction Prof.KFLau2 Sept.16 DayafterMid-AutumnFestival

3 Sept.23 Water Prof.KFLau4 Sept.30 Carbohydrates Prof.KFLau5 Oct.7 Lipids Prof.KFLau6 Oct.14(F1) NucleotideandNucleicAcids Prof.KFLau6 Oct.14(F2-

F3)Proteins Prof.KB

Wong7 Oct.21 Proteins Prof.KB

Wong8 Oct.28(F1-

F2)Mid-termExamination Prof.KFLau

8 Oct.28(F3) ProteinFunctions Prof.KBWong

9 Nov.4 Thermodynamics,EnergyandMatters

Prof.KBWong

10 Nov.11 EnzymeCatalysis Prof.KBWong

11 Nov.18 ChemicalLogicofBiochemicalReaction

Prof.KBWong

12 Nov.25 Glycolysis,Vitamins Prof.KBWong

13 Dec.2 OverviewofMetabolism Prof.KBWong

8. PostingCourseAnnouncementsPayattentiontoyourCUHKemailaccountforannouncementsaboutthiscourse,teacherswillsendmessagestostudentsviatheCUeLearningSystem(http://www.cuhk.edu.hk/eLearning/)andstudentscanviewthemessageautomaticallyintheirCUemailaccounts.Theclickersystemwillbeusedandmultiplechoicequestionswillbediscussedtoreinforcesomebasicconceptscoveredinthelectures.

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BCHE2070ResearchInternship(2016-17,1st Term / 2nd Term)

DescriptionThis course is designed to allow students to gain practical experience in scientificresearch in a laboratory or biotechnology firm during the summer period or termtime.All researchor internshipprojects, locallyoroverseas,mustbeapprovedbytheSchool.

LearningOutcomeAftercompletingthecourse,studentsshouldbeableto:1. experiencetocarryoutanindependentresearchundersupervisionbyafaculty

staff;2. design simpleexperimentsand followprotocols to carryout somebiochemical

analyses;3. obtaindataandcarryoutdataanalysiswithsimplestatistics,and4. learnhowtowriteabriefscientificreport.

Objectivesforstudentsdevelopment:1. Learn how to integrate basic biochemistry techniques to perform some

experimentsordevelopanassay.2. Learnhowtocollectandreadliteratureinaspecificareaofresearch.3. Learnhowtodesignsimpleexperiments,carryouttheexperimentalprocedure

independently,andworkasamemberoftheteam.4. Learnhowtocompileorcollectdatawithaccuracyandprecision.5. Learnhowtoanalyze thedataobtainedandwritea shortpaper to summarize

theexperimentsorworkdone.6. Better prepare students to learn timemanagement skills and do independent

researchfortheirfinalyearproject(SupervisedResearch)

CourseSyllabusThereisnospecificcontentforthiscoursewhichisguidedbyasupervisorandtheprojectistobeassignedbythesupervisor.SummerInterns(includingDREAM)maytakethiscoursebutalocalsupervisororsupervisorfromBiochemistryProgramhasto be assigned in addition to the internship supervisor for marking. Title of theprojectreportmustbedifferentfromothercourses.

Duration(studyperiod):Studentstakingthiscourseusuallystarttheirexperimentsinthesummerorearlyattermstartandcompletetheprojectwithareportwrittenformarkingbytheendoffirstterm.Deadlineofsubmissionofreport isattermend.Twomarkers(includingthesupervisorandanextramarkerisneededfordualsupervisors)willevaluatethereport(assessmentformattached).

Jobnature:

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Studentsmayholdaprojectontestingandpurificationofabiomolecule,cloningofagene, detection of a gene mutation, performing PCR assay or ELISA assay,purificationofarecombinantprotein,developinganassayfrommodifiedprotocols,doingliteraturesearchofpatentsandhelpfilingapatentapplicationinapatentlawfirm,doingdatamining (InSilicoanalyses)orsequenceanalyses, testingofadrug,etc.

AssessmentSchemeResearchreport 100%

RequiredReadingsTobeassignedbysupervisor.

RecommendedReadingsWebThiswebsiteexplainhowtowriteascientificreport:http://geog.arizona.edu/~comrie/geog230/report.htm

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BCHE3030MethodsinBiochemistry(2016-17,2ndterm)

DescriptionThis course aims at introducing quantitative analyses of biochemical reactionsandsubcellularcomponentstostudents.Methodsforpurificationandstudiesonbiomolecules will be introduced. Techniques including fluorescencespectrophotometry and microscopy, X-ray crystallography, centrifugation,chromatographyandelectrophoresiswillbecovered.Content/FundamentalConceptsFluorescenceTechnology:Thisblockisintendedtointroducetostudentsanin-depth knowledge of the principles of fluorescence and its applications forbiochemistryandcellbiologyresearch.Topicsincludeprinciplesoffluorescence,design and application of fluorescence probes, fluorescence measurements,specialtechniquesandrecentadvancesinfluorescencetechnology.X-rayCrystallography:Thissessionwillintroducethebasicprinciplesofproteincrystallization.Differentcrystallizationmethodsandoptimizationstrategieswillbe discussed. Theuse of X-raydiffraction to determine the three-dimensionalstructureofproteinswillbeintroduced.Centrifugation:Thissessionwillintroducethebasictheoryofcentrifugationandthe mathematical expression of sedimentation rate. We will also discuss thedifferent types of centrifuge and rotor commonly used in biochemical studies.Theprinciplesofdifferentialcentrifugationanddensitygradientcentrifugation,andtheirapplicationswillbecovered.Chromatography: This session will introduce the basic principles ofchromatography,apartitionprocessinwhichmoleculesdistributebetweentwodifferentphases.Thevariouschromatographictechniquesparticularlyusefulinprotein purification, including ion-exchange chromatography, gel filtration andaffinitychromatography,willbediscussed.Electrophoresis: In thisblockof lectures,particularemphasiswillbegiventothe electrophoretic methods used in studying proteins, namely SDS-polyacrylamide gel electrophoresis and isoelectric focusing. The use ofelectrophoresis in thestudyofnucleicacidswillbediscussed. Inaddition, theapplicationsoftheinstrumentaltechniquecapillaryelectrophoresiswillalsobecovered.LearningOutcomeAfter completing the course, students should be able to understand both thebasic principles and the practical aspects of different methods in biochemicalanalyses, including fluorescence techniques, X-ray crystallography,

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centrifugation,chromatographyandelectrophoresis.Theknowledgegainedwillbeusefulintheirfinalyearexperimentalresearchproject.AssessmentScheme ClassTest(I) 30% FluorescencetechnologyClassTest(II) 30% X-raycrystallography ClassTest(III) 40% Centrifugation,chromatographyandelectrophoresis LearningResourcesTextbook:Principles and Techniques of Biochemistry andMolecular Biology 7th edition,2010EditedbyKeithWilsonandJohnWalker,CambridgeUniversityPress(QP519.7P75)Reference:Other readingmaterials (including reference books, journals articles and websites)willbegivenbyindividualteachers.CourseScheduleT3-4 10:30am–12:15pm MMWLT2H4 11:30am–12:15pm MMWLT2Weekno. Date Hour Topic

1 10Jan(Tue)

2 IntroductiontoFluorescence SKKong

12Jan(Thu)

1 DesignandApplicationofFluorescentProbes

SKKong

2 17Jan(Tue)

2 Fura-2,aGoodExampleforFluorescentProbeDesign

SKKong

19Jan(Thu)

1 FluorescenceMeasurementI:FluorescenceFluorometer,Epi-FluorescenceMicroscope&ConfocalMicroscopy

SKKong

3 24Jan(Tue)

2 FluorescenceMeasurementII:Flow Cytometry, Real-time PCRMachine

SKKong

26Jan(Thu)

1 SpecialTechniquesandRecentAdvancesinFluorescenceTechnologyI:FluorescenceResonanceEnergyTransfer

SKKong

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4 31Jan(Tue)

0 Holiday:LunarNewYear

2Feb(Thu)

0 Holiday:LunarNewYear

5 7Feb(Tue) 2 SpecialTechniquesandRecentAdvancesinFluorescenceTechnologyII:GreenFluorescenceProtein&itsApplication

SKKong

9Feb(Thu)

1 IntroductiontoProteinX-rayCrystallography

ShannonAu

6 14Feb(Tue)

2 BasicPrinciplesinProteinCrystallizationandCrystallizationMethods

ShannonAu

16Feb(Thu)

1 CLASSTEST(I) SKKong

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7 21Feb(Tue)

2 CrystallizationOptimization ShannonAu

23Feb(Thu)

1 BasicConceptsinX-rayDiffraction(I) ShannonAu

8 28Feb(Tue)

2 BasicConceptsinX-rayDiffraction(II) ShannonAu

2Mar(Thu)

1 StructureDetermination ShannonAu

9 7Mar(Tue)

2 ModelingBuildingandRefinement,RecentadvancesinProteinCrystallography

ShannonAu

9Mar(Thu)

1 BasicPrinciplesinCentrifugation WPFong

10 14Mar(Tue)

2 DifferentCentrifugationTechniques WPFong

16Mar(Thu)

1 CLASSTEST(II) ShannonAu

11 21Mar(Tue)

2 BasicPrinciplesinChromatography WPFong

23Mar(Thu)

1 ResolutioninChromatographicSeparation

WPFong

12 28Mar(Tue)

2 DifferentModesofChromatographicSeparation

WPFong

30Mar(Thu)

1 ChromatographyinProteinPurification

WPFong

13 4Apr(Tue) 0 Holiday:ChingMingFestival

6Apr(Thu)

1 Electrophoresis:SDS-PAGE WPFong

14 11Apr(Tue)

2 FactorsAffectingElectrophoreticMobility

WPFong

13Apr(Thu)

1 DetectionofProteinafterElectrophoresis

WPFong

15 18Apr(Tue)

2 IsoelectricFocusing,2-DElectrophoresis,ElectrophoresisofNucleicAcids

WPFong

20Apr(Thu)

1 CapillaryElectrophoresis WPFong

Teacher’sContactDetails

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ProfessorFONG,WingPing(coordinator)MMW608 39436868wpfong@cuhk.edu.hkProfessorKONG,SiuKai MMW609 39436799skkong@cuhk.edu.hkProfessorAU,ShannonW.N. SC178 39434170shannon-au@cuhk.edu.hkAFacilityforPostingCourseAnnouncementsCourseannouncementsandmaterialswillbepostedontheBlackboardwebsiteattheCUE-Learningsystem.Thepowerpointslidesusedwillbepostedonthewebsitebeforethelecture.Nohardcopieswillbedistributedintheclass.

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BCHE3040ProteinsandEnzymes(2016-17,1stTerm)

DescriptionEnzyme catalysis will be introduced with respect to coenzyme requirements,kinetics, catalytic mechanism and regulation of enzymatic activities. Three-dimensional conformations of proteins and protein foldingwill be discussedwithemphasisonstructure-functionrelationships.Contents/FundamentalConceptsIntroduction toEnzymes: Thebasiccharacteristicsofenzymesasbiocatalysts, forexample the specificity, ability to be regulated and the catalytic power will beintroduced. Ribonucleic acids having catalytic activities will be mentioned. Theenzyme nomenclature system and the requirement of coenzymes andmetals ionsforvariousenzymaticreactionswillalsobediscussed.Enzyme Kinetics: Different methods for measuring enzyme activities and theprecautionsneededwhenperformingenzymeassayswillbediscussed. Michaelis-Mentenenzyme steady statekineticswill be covered,withparticular emphasisonthe significance of the Km and kcat values. Different types of enzyme inhibitors,includingallostericones,willbe introduced. Thevariousmeansbywhichenzymeactivities canbe regulatedwillbementioned. Exampleswillbegiven to illustratehowthedifferentaminoacidresiduesintheactivesiteoftheenzymecontributetotheactivityoftheenzyme.ClinicalandIndustrialEnzymology:Thissessionwillintroducetheuseofenzymesindifferentclinicalapplications, forexample, theserumenzymeactivityassayasadiagnostic tool, the use of enzyme as a reagent to measure the concentration ofmetabolites, the use of enzyme inhibitor as drug and the use of enzyme itself astherapeutic agent. Examples will also be given to illustrate the application ofenzymesindifferentindustries,particularly,inpharmaceuticalandfoodindustries.Protein Structure and Folding: This session will discuss the basic principlesgoverning the structure of protein. As protein molecules are organized in astructural hierarchy, topology diagrams will be used to illustrate the motifs ofprotein structure. In particular, the alpha-domain structures, beta structures andthealpha/betastructureswillbediscussed. Thekeyconceptsof thermodynamicsand kinetics in the process of protein folding and the stabilization of proteinstructurewillbeillustrated.Structure-FunctionRelationshipinSelectedProteins:Specificexampleswillbeusedtoillustratethegeneralstructureofmembraneproteins.Chaperoneisproteinthathelpscorrectproteinfoldingwhileubiquitin,togetherwiththeproteasomesystem,isresponsibleforproteindegradation.Theywillbediscussedwithrespecttotheirstructure-function relationship. Transcription factors are DNA-binding proteinsthat regulate gene activity. Different DNA binding motifs will be introduced.

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Proteinscanbeengineeredtoimprovetheirproperties.MethodslikeDNAshufflingwillbediscussed.Protein-Protein Interaction: The different methods for studying protein-proteininteraction will be introduced. Among them are qualitative ones like co-immunoprecipitation, chemical cross linking, yeast twohybrid system, fluorescentresonance energy transfer, and also quantitative ones like surface plasmonresonanceandisothermaltitrationcalorimetry.Theuseofmassspectrometryintheidentificationofproteinwillalsobediscussed.LearningOutcomesAftercompletingthecourse,studentsshouldbeableto:(1) knowthebasicprinciplesinenzymology;(2) understandthekinetics,mechanismandregulationofenzymeactivity;(3) realizetheclinicalandindustrialapplicationsofenzymes;(4) explaintheproteinstructureandthefoldingprocess;(5) learn the structure and function of some selected proteins, for example,

membraneproteins;and(6) describethedifferentmethodsforstudyingprotein-proteininteractions.AssessmentSchemeAssignment: Enzymes: 10%Mid-termExamination: Enzymes: 45%FinalExamination: Proteins: 45%LearningResourcesReferences: (1) Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic

Proteins.N.C.PriceandL.Stevens,OxfordUniversityPress,1999.(QU135P83)

(2) Proteins:StructureandFunction.D.Whitford,JohnWiley&SonsLtd.,2005.(QU55W535)

(3) IntroductiontoProteins:Structure,FunctionandMotion.A.KesselandN.Ben-Tal,CRCPress,2011.(QU55K37)

(4) IntroductiontoProteinStructure.C.BrandenandJ.Tooze,GarlandPub.,1999.(QU55B76)

(5) Biochemistry.J.M.Berg,J.L.TymoczkoandL.Stryer,W.H.Freeman,2012.(QU4S77)

(6) LehningerPrinciplesofBiochemistry.D.L.NelsonandM.M.Cox,W.H.Freeman,2008.(QD415L44)

(7) PrinciplesandTechniquesofPracticalBiochemistry.K.WilsonandJ.Walker,CambridgeUniversityPress,2010.(QP519.7P75)

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BCHE3040ProteinsandEnzymes2016-17,1stTerm

CourseScheduleM1 8:30am-9:15am MMWLT2W1-2 8:30am-10:15am MMWLT2a. CourseScheduleWeek Date Hour(s) Topic

1 5Sep(Mon) 0 InaugurationCeremonyforUndergraduates–NOCLASS

7Sep(Wed) 2 IntroductiontoEnzymes

2 12Sep(Mon) 1 RNAasBiocatalyst

14Sep(Wed) 2 CofactorRequirement

3 19Sep(Mon) 1 DeterminationofEnzymeActivity

21Sep(Wed) 2 Michaelis–MentenKinetics

4 26Sep(Mon) 1 EnzymeInhibitors

28Sep(Wed) 2 Bi-substrateReactionsandPre-steadyStateKinetics

5

3Oct(Mon) 1 AllostericEnzymeandMultimericEnzyme

5Oct(Wed) 2 RegulationofEnzymeActivity

6 10Oct(Mon) 0 PUBLICHOLIDAY

12Oct(Wed) 2 MechanismofEnzymeCatalysis

7

17Oct(Mon) 1 ExamplesofEnzymeMechanism

19Oct(Wed) 2 ClinicalEnzymology

8

24Oct(Mon) 1 IndustrialEnzymology

26Oct(Wed) 2 IntroductiontoProteinStructure

9

31Oct(Mon) 1 Super-secondaryStructure

2Nov(Wed) 2 MID-TERMEXAMINATION

10 7Nov(Mon) 1 ProteinFolding

9Nov(Wed) 2 ProteinsAssociatedwithProteinFoldingandDegradation

11 14Nov(Mon) 1 MembraneProteins

16Nov(Wed) 2 Bacteriorhodopsin,PotassiumChannelandGProtein

21

BCHE3040PROTEINSANDENZYMES2016-17,1stTerm

12 21Nov(Mon) 1 DNA-bindingProtein

23Nov(Wed) 2 ProteinEngineering

13 28Nov(Mon) 1 Protein-ProteinInteraction

30Nov(Wed) 2 ProteinIdentificationandMassSpectrometry

Total: 37

b.TutorialScheduleQuestionandAnswerSession(s)willbearranged.Teacher’sContactDetailsProfessorFONGWING-PINGMMW60839436868wpfong@cuhk.edu.hkAFacilityforPostingCourseAnnouncementsCourse announcements and materials will be posted on the Blackboard coursewebsite. The powerpoint slides used will be posted on the Blackboard coursewebsitebeforethelecture.NOhardcopieswillbedistributedintheclass.AcademicHonestyandPlagiarismAttention is drawn toUniversity policy and regulations on honesty in academicwork,andtothedisciplinaryguidelinesandproceduresapplicabletobreachesofsuchpolicyand regulations. Details may be found athttp://www.cuhk.edu.hk/policy/academichonesty/.

22

BCHE3050MolecularBiology(2016-17,2ndTerm)

DescriptionThiscoursediscussesbasicareasofmolecularbiology,includingcharacteristicsofDNAandRNA,genomicelements,DNAreplication,transcription,translation,mutation,repair,generegulationandepigenetics.LearningOutcome1.Tounderstandthebasicphenomenaoflifeinthemolecularbiologylevel,whichincludethecharacteristicsofDNAandRNA,genomeandelements,DNAreplication,DNAmutationandrepair,transcription,translation,generegulationandepigenetics.2.Toknowthemajordifferencesoftheabovephenomenabetweenprokaryoticandeukaryoticorganisms.CourseSyllabus1.CharacteristicsofDNAandRNA,2.Eukaryoticgenomeandelements,3.DNAreplication,4.DNAmutation,5.DNArepair,6.TranscriptionofDNA,7.RNAprocessing,8.Transcriptionalregulation,9.Proteintranslation,10.Translationalcontrol,11.Epigenetics.EnrollmentRequirementsNotforstudentswhohavetakenBCHE4010orBIOL2310.

23

BCHE3050MolecularBiology(2016-17,Term2)

TimeandVenue:

Fri9:30am–11:15am,LSKLT3

Convenor: Prof.DianeGUODianjing(convenor) RmG81,ScienceCenter Tel:3943-6298 Email:djguo@cuhk.edu.hk

Topic Lecture MeetingDate

• Introduction 1,2 13/1• StructureofDNA,RNA,andchromosome 3,4 20/1

• DNAreplication 5,6 3/2

• Transcription 7,8 10/2

• Transcription 9,10 17/2

• Translation 11,12 24/2

• Midtermexam 3/3• Regulationofgeneexpression 13,14 10/3• Regulationofgeneexpression 15,16 17/3

• GeneMutationandDNArepair 17,18 24/3

• RecombinationandTransposition 19,20 31/3

• RecombinantTechnology 21,22 7/4

• Tutorials 23,24

21/4

Assessment:Mid-term:45%,Final:55%Mid-termExamination(Lectures1-12) (1hr.)FinalExamination(Lectures13-26) (2hrs.)MajorReference: 1.Brooker,RobertJ.2015.Genetics:analysisandprinciples.5rded.McGraw.Hill2.Klug,WS&Cummings,MR.2006.ConceptsofGenetics.8thed.MacMillan3.Snustad,DP&Simmons,MJ.2006.PrinciplesofGenetics.4thed.J.Wiley.

24

BCHE3070/BCHE4020RecombinantDNATechniques(2016-17,2ndTerm)

Week Date Topic Teacher1 12Jan Restrictionandmodificationenzymes PCS2 29Jan Restrictionandmodificationenzymes PCS3 26Jan SeparationandjoiningofDNA,purificationofDNA,

shortexercise1PCS

4 2Feb ChineseNewYearHoliday -5 9Feb Submitexercise,Prokaryoticvectorsandtheir

introductiontocellsPCS

6 16Feb Discussiononexercise PCS7 23Feb Prokaryoticvectorsandtheirintroductiontocells PCS8 2Mar Polymerasechainreaction,DNAsequencingand

applicationsPCS

9 9Mar Polymerasechainreaction,DNAsequencingandapplications,shortexercise2

PCS

10 16Mar Eukaryoticvectorsandtheirintroductiontocells PCS11 23Mar Submitexercise,toobtainclonesofaspecificgene

ProductionofproteinfromclonedgenePCS

12 30Mar Discussiononexercise Tutor13 6Apr Productionofproteinfromclonedgene PCS14 13Apr Studyinggeneexpressionandfunction PCS15 20Apr Test(45min–opennote) PCS/Tutor

ClassPeriod:10:30AM-11:15AM(Thursday),MMWLT1CoursedescriptionThiscoursecoversthebasicconceptsofcommonrecombinantDNAtechniques,includingrestrictionandmodificationenzymesandtheirapplicationsinrecombinantDNAtechniques,analysesofnucleicacids,insertionofDNAfragmentintovectorsandtransformationofmodelorganisms,expressionofrecombinantproteins,polymerasechainreaction,DNAandgenomesequencingandstudyofgeneexpressionandfunction.Courseobjectives(1)knowthebasiccommontechniquesforDNAmanipulationandanalyses,(2)applytheknowledgeacquiredongivencases.ReferenceBrown,T.A.(2010)GenecloningandDNAanalysis.Anintroduction.6thEdition.AssessmentschemeExercise:20%,Opennotetest:80%

25

BCHE3080BioenergeticsandMetabolism(2016-17,1stterm)

Teacher Office Tel. E-mailDr.NgaiHung-Kui,Patrick MMW507B 39434359 hkngai@cuhk.edu.hk

MeetingInformationMonday 09:30-10:15 MongManWaiBuildingLT1Friday 08:30-10:15 MongManWaiBuildingLT2CourseDescription

This course covers basic concepts of how biological organisms obtain energy and performmetabolism to build different biomolecules. The following biochemical processes underlyingenergytransformationinlivingorganismswillbediscussed:photosynthesis,glycolysis,citricacidcycle, electron transport, oxidative phosphorylation, fatty acid oxidation. Biosynthesis ofcarbohydrates, lipids,nucleicacids,andproteinswillbeprovided.The integrationofmetabolicpathways will also be discussed. Students are advised to take BCHE2030 before taking thiscourse.AssessmentSchemeMid-TermExamination 30%FinalExamination 60%TermPaper(~600words) 10% RecommendedTextbooks1. David L. Nelson, Michael M. Cox. (2013). Lehninger Principles of

Biochemistry(6thed.).NewYork:W.H.Freeman.[UL:QU4.N452013]2. JeremyM. Berg, John L. Tymoczko, Lubert Stryer. (2012).Biochemistry (7th

ed.).NewYork:W.H.Freeman.[UL:QU4.S772012]3. Thomas M. Devlin. (2011). Textbook of biochemistry with clinical

correlations (7th ed.). Hoboken, NJ: JohnWiley & Sons. [UL: Oversize - QU4.T492010]

4. DonaldVoet,JudithG.Voet.(2011).Biochemistry(4thed.).Hoboken,NJ:JohnWiley&Sons.[UL:Oversize-QP514.2.V642011]

ElectronicResources(E-Books)1. PhilipW.Kuchel, SimonEasterbrook-Smith,VanessaGysbers, J.Mitchell

Guss.(2012).Biochemistry(3rded.).NewYork:McGraw-Hill.PermanentURLforthisrecord:http://library.cuhk.edu.hk/record=b4968891~S8

2. JohnW.Pelley.(2012).Elsevier'sintegratedreview.Biochemistry(2nded.).

Philadelphia:Elsevier/Saunders.PermanentURLforthisrecord:http://library.cuhk.edu.hk/record=b5073806~S8

3. GerhardMeisenberg,William H. Simmons (2012). Principles ofmedical

biochemistry(3rded.)Philadelphia:Elsevier/Saunders.

26

Permanent URL for this record:http://library.cuhk.edu.hk/record=b5329956~S8ElectronicResources(E-Journals)1. Biologicalchemistry(Berlin;NewYork:W.deGruyter)

PermanentURLforthisrecord:http://library.cuhk.edu.hk/record=b2813130~S3

2. Biochemistry(NewYork:MAIKNauka/Interperiodica)PermanentURLforthisrecord:http://library.cuhk.edu.hk/record=b2813087~S3

3. Cellmetabolism(Cambridge,MA:CellPress] Permanent URL for this record:http://library.cuhk.edu.hk/record=b4058128~S34. The journal of clinical endocrinology & metabolism (Baltimore, MD:EndocrineSociety)

PermanentURLforthisrecord:http://library.cuhk.edu.hk/record=b2821335~S3

CUeLearningSystem(Blackboard)Visit the website of the CU eLearning System (https://elearn.cuhk.edu.hk/) andloginusingyourStudentIDandCWEMpassword.1. CourseWebsite 2016R1-BCHE3080:BioenergeticsandMetabolism2. CompanionWebsite

CU-BCHE3080-EL:BioenergeticsandMetabolism(ExtendedLearning)HonestyinAcademicWorkEveryassignmenthanded inshouldbeaccompaniedbyasigneddeclaration.Theformcanbedownloadedviathefollowingwebsite.Assignmentswithouttheproperlysigneddeclarationwillnotbegraded.http://www.cuhk.edu.hk/policy/academichonesty/Eng_htm_files_(2013-14)/declaration_en.doc

27

BCHE3080BioenergeticsandMetabolism

(2016-17,1stterm)

CourseScheduleDate Hr. Modules

Sept.5(M)

- Inaugurationceremonyforundergraduates,classessuspendedinthemorninguntil1:30pm

Sept.9(F) 2 Module1OverviewofBioenergeticsandMetabolismSept.12(M)

1

Sept.16(F)

- PublicHoliday–ThedayfollowingtheChineseMid-AutumnFestival

Sept.19(M)

1 Module2GlycolysisandtheCatabolismofHexoses

Sept.23(F)

2 ! Glycolysis:acentralpathwayofglucosemetabolism! Utilizationofothersugarsasmetabolicfuels! Pentosephosphatepathway:productionofreducingpowderand

formationofpentosesugarsSept.26(M)

1 Module3TheCitricAcidCycle

Sept.30(F)

2 ! Commonpathwayforoxidationofacetylgroup! RegulationandamphibolicnatureofTCAcycle

Oct.3(M) 1 Module4OxidativePhosphorylationOct.7(F) 2 ! Structureandfunctionofelectrontransportchain

! MechanismofATPsynthesisOct.10(M)

- PublicHoliday–ThedayfollowingtheChungYeungFestival

Oct.14(F)

2 Mid-termExamination(Modules1-4)

Oct.17(M)

1 Module5Photophosphorylation! Structureofphotosynthesisapparatus! ThegenerationofATPandtheCalvincycleOct.21

(F)2

Oct.24(M)

1 Module6OxidationofFattyAcids

Oct.28(F)

2 ! Utilizationoffattyacidsasmetabolicfuels! Degradativepathwayforfattyacidofevenoroddnumberofcarbons! Formationandutilizationofketonebodies

Oct.31(M)

1 Module7OxidationofAminoAcidsandtheUreaCycle

Nov.4(F) 2 ! Pathwaysofaminoaciddegradation! Metabolicfatesofaminogroups! Nitrogenexcretionandtheureacycle

Nov.7(M)

1 Module8BiosynthesisofCarbohydrates

Nov.11 2 ! Gluconeogenesis

28

(F) ! BiosynthesisofglycogenandothercarbohydratesNov.14(M)

1 Module9BiosynthesisofLipids

Nov.18(F)

2 ! Biosynthesisoffattyacidsandtriacylglycerol! Biosynthesisofmembranephospholipids! Biosynthesisofcholesterol,steroidsandisoprenoids

Nov.21(M)

1 Module10BiosynthesisofAminoAcids

Nov.25(F)

2 ! Biosynthesisofaminoacids! Moleculesderivedfromaminoacids

Nov.28(M)

1 Module11IntegrationofMetabolism

Dec.2(F) 2 Module12NucleotideMetabolism ! Biosynthesisanddegradationofnucleotides

DiscussionFirstTermPeriod:5September2016(Mon)–3December2016(Sat)

[END]

29

BCHE3090Self-studyModulesinBiochemistry(2016-17,1stTerm)

CourseCoordinator:Prof.WNAuTeachers:Prof.KMChan,Prof.TFChan,Dr.FHLo,Dr.HKNgai,Prof.CKNgoCOURSEOBJECTIVESThis course aims at training students to critically read and present scientificpapers. Life-long self-learning abilities in information search, critical analysisand communications are our emphasis. Through different course activities,students should also be able to develop their generic skills in collaborativeteamwork,problemsolving,andpeer-assessment.

COURSEOPERATIONThis course will include lectures and presentation sessions. Students arerequiredtogivetwogrouporalpresentations(I&II)andsubmitoneindividualwrittenreport.GrouppresentationI1. Each presentation group (two students per group) will give an oralpresentationonanassignedresearchpaper.Matchingoftheresearchpaperswillbemadebyrandomdrawon6Sep2016.

2. Each oral presentation will be in 20-min, followed by a 10-min opendiscussion.Onemarkwillbedeductedforeachminuteofoverrun.

3. All students are required to read the papers and actively participate in thediscussion.

4. Each presentation group is required to hand in a draft Powerpoint file andmeettheirteacheron4Oct2016.(Note:Failure tomeet the teacheron4Oct2016willreceivezeromarkforthecorrespondingassessment.Itisthe responsibility of the students to take the initiative to makeappointmentwiththeirteacher.)

5. Presentation file needs to be uploaded to Blackboard 24-hr before theassigneddateoforalpresentation.Foranylatesubmission,twomarkswillbedeductedfromthefinalscore.

6. Guidelinesfortheoralpresentationareattached.

GrouppresentationII1. Each group is required to give an oral presentation on a selected researcharticlepublishedwithin3yearsinoneofthejournalsinAppendixI.StudentsshouldsubmitthearticletoBlackboardonorbefore28Oct2016.Foranylatesubmission,twomarkswillbedeductedfromthefinalscore.

2. Presentation file needs to be uploaded to Blackboard 24-hr before thepresentation.Forany latesubmission, twomarkswillbededucted fromthefinalscore.

30

3. Each oral presentation will be in 20-min, followed by a 10-min opendiscussion.Onemarkwillbedeductedforeachminuteofoverrun.

4. All students are required to read the papers and actively participate in thediscussion.

AttendanceoftheoralpresentationTheoralpresentationwillstartat10.30am.Forstudentswhoarelatefortheirpresentations,noextratimewillbecompensated(e.g.ifastudentislatefor10minutes,themaximumtimeallowedforhis/herpresentationwillbe10minutes).Onesub-grade(e.g.A-toB+)willbedeductedwhenastudentfailstoattendtwogrouporalpresentations.Individualwrittenreport1. ThewrittenreportshouldbeonanarticleselectedinaspecificVirtualIssueofJournalofBiologicalChemistry.StudentswillbenotifiedwhichVirtualIssuecanbeusedforthewrittenreport.

2. Deadline for report submission is 5 p.m., 9 Dec 2016. For each day of latesubmission,twomarkswillbedeductedfromthefinalscore.

3. Formatofthewrittenreportcanbefoundintheattachedguidelines.

ASSESSMENT(100%)1. Draftpowerpointfileandmeetyourteacher:10%2.GrouppresentationI:30%• Presentation:20%• Peerassessment:5%• Participation(Q&Asession):5%

3.GrouppresentationII:30%• Presentation:20%• Peerassessment:5%• Participation(Q&Asession):5%

4.Individualwrittenreport:30%Remarks:1. Oralpresentationwillbeevaluatedbyteacherandalsopeerassessedbyparticipantsinthesamegroup.

2. Individualwrittenreportswillbeevaluatedbytwoteachers.3. Assessmentcriteriaarelistedintheassessmentforms.

31

BCHE3090Self-studyModulesinBiochemistry(2016-17,1stTerm)

Teachers Office Tel. E-mailProf.ShannonW.N.Au(Coordinator)

WNA SC178 39434170 shannon-au@cuhk.edu.hk

Prof.T.F.Chan TFC SC177 39436876 tf.chan@cuhk.edu.hkProf.K.M.Chan KMC SC184 39434420 kingchan@cuhk.edu.hkDr.F.H.Lo FHL MMW507B 39435019 lofaihang@cuhk.edu.hkDr.PatrickH.K.Ngai HKN MMW507B 39434359 hkngai@cuhk.edu.hkProf.JackyC.K.Ngo CKN SCE403 39436346 jackyngo@cuhk.edu.hk

ScheduleforGroupA-F TUESDAY(10:30am-12:15pm)Wk. Date Topic Teacher/Venue Remarks1 6Sep Introduction WNA/MMWLT2 ReviewModule62 13Sep Howtoreadand

presentascientificpaper

WNA/MMWLT2 ReviewModule6

3 20Sep Self-study - ReviewModule1-5&7

4 27Sep Self-study - ReviewModule1-5&7

5 4Oct Meetyourteacher WNA/KMC/TFC/FHL/HKN/CKN

Prepareadraftpowerpointfileandmeetwithyourgroupteacher

6 11Oct GrouppresentationI–AssignedPaperI

WNA(GroupA):LSK203KMC(GroupB):ERB406TFC(GroupC):MMW706FHL(GroupD):MMW715CKN(GroupE):MMW622HKN(GroupF):LHCG01

7 18Oct GrouppresentationI–AssignedPaperII

WNA(GroupA):LSK203KMC(GroupB):ERB406TFC(GroupC):MMW706FHL(GroupD):MMW715CKN(GroupE):MMW622HKN(GroupF):LHCG01

8 25Oct Self-study Submityourselectedarticleby28Oct2016,5pm

9 1Nov Writingascientificpaper

CKN:ICSL1

10 8Nov Self-study - 11 15Nov GrouppresentationII

–Selectedpapers

HKN(GroupA):LSK203WNA(GroupB):ERB406KMC(GroupC):MMW706TFC(GroupD):MMW715

32

FHL(GroupE):MMW622CKN(GroupF):LHCG01

12 22NovGrouppresentationII–Selectedpapers

HKN(GroupA):LSK203WNA(GroupB):ERB406KMC(GroupC):MMW706TFC(GroupD):MMW715FHL(GroupE):MMW622CKN(GroupF):LHCG01

13 29Nov Self-study Submityourwrittenreportby9Dec2016,5pm

33

BCHE3650MolecularBiologyandRecombinantDNALaboratory(2016-17,2ndTerm)

DescriptionA set of experiments to illustrate various molecular biology and recombinantDNAtechniqueswillbeperformed.TheseincluderecombinantDNAconstructionand characterization, DNA sequencing and analyses, DNA authentication, geneexpressionstudy.StudentsarepreferredtoattendBCHE3050andBCHE3070.LearningOutcome1. Students should be able to perform simple experiments using molecular

biology and recombinant DNA techniques such as DNA preparation,construction and analyses of recombinant DNA, generation of cDNA, DNAsequencingandpolymerasechainreaction.

2. StudentsshouldbeabletograspthebasicconceptofmolecularbiologyandrecombinantDNAtechniques.

3. Knowledge gained from this course is useful for the final year courseSupervisedResearchinBiochemistryandotherresearchwork.

CourseSyllabusRecombinantDNAconstruction,DNAtransformation,plasmidDNApreparation,paternitytest,DNAsequencing,bioinformaticanalyses,geneexpressionstudy.CourseScheduleDate Experiment ContentJan.12 CheckinJan.19 1 DNAExtractionandPolymerasechainreactionFeb.9 2 RecombinantDNAconstructionFeb.16 3 DNAtransformationintoE.coli.Feb.23 4 Plasmidpreparation(PartI)Mar.2 4and

6Plasmidpreparation(partII)Paternitytest(partI)

Mar.9 5and6

DNASequencingDemonstrationandBlastSearchPaternitytest(partII)

Mar.16 7 GeneExpressionstudy(partI)Mar.23 5and

7DNASequencingDemonstrationandBlastSearchGeneExpressionStudy(partII)

Mar.30 LabDiscussionApr.13 LabExamination

34

BCHE3650MolecularBiologyandRecombinantDNALaboratory(2016-17,2ndTerm)

AssessmentSchemeLabperformance: 10%Lab.reports: 25%Pre-classexercise: 10%Pre-labquiz: 10%Examination: 45%

35

BCHE3730AnalyticalBiochemistryLaboratory(2016-17,2ndTerm)

DescriptionThis laboratory course provides basic training in biochemical techniques. Studentswill learn the application of different biochemical methods in the qualitative andquantitativeanalysesofbiologicallyactivemolecules.ThiscourseisidenticaltotheBCHE3630MethodsinBiochemistryLaboratoryofferedin2014orbefore.LearningOutcomeStudentsareexpectedtolearndifferentmethodsforbiochemicalanalyses,includingchromatography, electrophoresis, protein crystallization, centrifugation andfluorescence techniques. Knowledgegained from this course is useful for the finalyearcourseSeniorExperimentalProjectandotherresearchwork.CourseSyllabusFluorescence Techniques: Because of its outstanding sensitivity, reliability, non-invasivenessandfriendlinesstoourenvironment,detectionof targetmoleculesbyusingfluorescencehasbeenacommontechnologyforvarioustypesofbiochemicaland biomedical studies. This session attempts to introduce to students differentfluorescencetechniquesincludingtheuseoffluorometer,fluorescenceplatereader,confocalmicroscope,andflowcytometerforanumberofbiochemicalanalyses.CrystallizationofLysozyme:TheX-raycrystallographyisaveryimportanttechniqueto study the three-dimensional structure of proteins. A good X-ray diffractionpattern,however, requiresa largeprotein crystal that is freeof contaminantsandaggregation.Inthisexperiment,studentswillacquirethebasicskillsofproducingacrystal of lysozyme. In addition, the effects of different protein and saltconcentrationsonlysozymecrystallizationwillalsobeinvestigated.Separation of Sub-cellular Components by Differential Centrifugation: Differentialcentrifugation will be used to separate sub-cellular components for biochemicalanalysis. Different sub-cellular components, such as the nucleus and the cellmembrane, have different sedimentation properties and therefore, they can bedifferentially sedimentedto thebottomof thecentrifuge tube. In thisexperiment,we will attempt to separate key cellular components (the nucleus, themitochondria/membrane and the cytosol fractions) using a simple centrifuge. Theexperimentwasdesignedtoshowthatbyvaryingtheparametersofcentrifugation,one could selectively separate cellular components into fractions with distinctivebiochemicalcharacteristics.Ion Exchange Chromatography: Chromatography is a techniquewhich depends onthedistributionofamoleculebetweentwodifferentphases,astationaryphaseandamobilephase.Dependingonthestationaryphaseused,therearedifferentkindsofchromatographywhichallowforseparationofmoleculeswithdifferentproperties.

36

In the experiment, ion exchange chromatography will be used to separate threedifferentproteins.SodiumDodecylSulfate-PolyacrylamideDiscontinuousGelElectrophoresis:SDSisananionicdetergentwhichbindsstronglytoproteinataconstantratiosuchthatalltheSDS-treated proteins are negatively charged. Besides having the same charge tomassratio,theSDS-treatedproteinsalsohavethesameshape.Consequently,whenelectrophoresedinthemolecularsievingpolyacrylamidegel,theywillbeseparatedaccording to sizeand theirmolecularweight canbedeterminedwith reference tothestandards.

CourseScheduleDate Experiment Content

11Jan. Checkin

25Jan.&8Feb.

1 TheuseofFura-2formeasurementoffreecalciumionconcentrationDemonstration:Celldeathanalysisbyflowcytometryandfluorescencemicroscopy

1Mar. 2 Crystallizationoflysozyme

22Mar. 3 Separationofsub-cellularcomponentsbydifferentialcentrifugation

29Mar. 4 Ionexchangechromatography

5Apr. 5 Sodiumdodecylsulfatepolyacrylamidegelelectrophoresis(SDS-PAGE)

12Apr. Lab.discussion

19Apr. Lab.examination

AssessmentSchemeReport: 50%Pre-labquiz: 10%Lab-log: 10%Performance: 5%Exam: 25%RequiredReadingsThe students will be given a laboratory manual with details, including the basicprinciplesandtheproceduresoftheexperiments.EnrollmentRequirementsCo-requisite:BCHE3030;NotforstudentswhohavetakenBCHE3630.

37

BCHE4030ClinicalBiochemistry(2016-2017,2ndTerm)

CourseCode: BCHE4030(3units) Period: M5(12:30pm–1:15pm);W1-2(8:30am-10:15am)Venue: SCL4MediumofInstruction: EnglishTeachers:FHLo MMWBRm507B,Tel:3943-5019,e-mail:lofaihang@cuhk.edu.hkSKKong(Coordinator) MMWBRm609,Tel:3943-6799,e-mail:skkong@cuhk.edu.hkObjectives:This course presents the basic principles in clinical biochemistry and its methodology. Test offunctions, biochemical profiles involved in pathogenesis, diagnosis and management ofsomediseaseswillbedescribed.LearningOutcomes:

Aftercompletingthecourse,studentsshouldbeableto:•Acquirecoreknowledgeonclinicalbiochemistry,•Understandtherelationshipofbiochemistrytoclinicalproblems;•Appreciatetheapplicationsofbiochemistrytomodernmedicine;•Applyknowledgeofclinicalchemistryfordiseasesdiagnosis;•Developgenericskillssuchascriticalthinking,writing&life-longlearningskillsand

teamspirit.

Wk Date/2017 Hr. Topics TeachereAdd-Drop:16–22Jan2017

1 Jan9(M) 1 •IntroductiontoClinicalBiochemistry•RevisionofAnalyticalMethods•BiochemicalEndocrinology•PrinciplesofEndocrineDisorders•LaboratoryInvestigationsofEndocrineDisorders•MetabolicAspectsofMalignantDiseases

LFH(25) Jan11(W) 22 Jan16(M) 1 Jan18(W) 23 Jan23(M) 1 Jan25(W) 24 Jan30(M) No-Class/ChineseNewYearHoliday Feb1(W) 5 Feb6(M) 1 •LiverFunctions

•LiverDiseasesandBiochemicalInvestigations•BloodCells,PlasmaProteinsandEnzymesI•Bloodcells,PlasmaProteinsandEnzymesII•HaemostasisandThrombosis•BloodDisordersandTests

Feb8(W) 26 Feb13(M) 1 Feb15(W) 27 Feb20(M) 1 Feb22(W) 28 Feb27(M) 1 Tutorial Mar1(W)* 2 Mid-termTestI(Venuetobeconfirmed)9 Mar6(M) 1 •RenalFunctionsandBoneProfile

•IronProfile,LipidProfile,andCardiacMarkers Mar8(W) 210 Mar13(M) 1 Tutorial Mar15(W)* 2 Mid-TermTestII(Venuetobeconfirmed)11 Mar20(M) 1 •ElectrolytesandWaterBalance SKK(14) Mar22(W) 2 •ElectrolytesandWaterBalance

38

12 Mar27(M) 1 •DisordersofElectrolytesandWaterBalance Mar29(W)* 2 QuizI

•Acid-BaseRegulation13 Apr3(M) 1 •Acid-BaseRegulation Apr5(W) 2 •DisordersofAcid-Base14 Apr10(M) 1 •DisordersofAcid-Base Apr12(W)* 2 QuizII

•DevelopmentofNewClinicalBiochemistryAssays15 Apr17(M) Noclass/EsterHoliday April19(W) 2 •DevelopmentofNewClinicalBiochemistryAssays

CourseEvaluation(10:00am–10:15am)*:Quiz

References:GeorgeJ.Netto,RanaD.Saad,PeterA.Dysert,II.Diagnosticmolecularpathology:currenttechniquesandclinicalapplications,partI.Proc(BaylUnivMedCent)2003October;16(4):379–383.

(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214554/)Otherreferencematerialswillbeprovidedbyteachers.

Textbooks:(AllarereservedintheLibrary)" HerbertWaldmann&PetraJanning.Conceptsandcasestudiesinchemicalbiology.(online)" JohnWBaynes&MarekHDominiczak.MedicalBiochemistry,4thed.(online)" Lela Buckingham & Maribeth L. Flaws. Molecular Diagnostics: Fundamentals, Methods, &

ClinicalApplications.(online)" MichaelLiebermanetal.Marks'basicmedicalbiochemistry:aclinicalapproach,4thed.(online)" VictorHoffbrand&PaulMoss.EssentialHaematology,6thed.(online)" WilliamJMarshall&StephenKBangert.ClinicalChemistry,6thed.(online)" MichaelLaposata.LaboratoryMedicineTheDiagnosisofDiseaseintheClinicalLaboratory,2nd

ed.(ULQY25.L2852014)• NessarAhmed.ClinicalBiochemistry.(QU4.C552011)(MedicalLibrary)• PhilipD.Mayne.ClinicalChemistryinDiagnosisandTreatment,6thed.(QY90.Z541994)• Shauna C. Anderson & Susan Cockayne. Clinical Chemistry: Concepts and Application. (UL

OversizeQY90.C64232007)Assessment: Mid-termTestI: ShortQuestions(close-bookformat)30%finalmarkMid-termTestII: MCQ(close-bookformat)20%finalmarkQuizI: 10%finalmark(SKKmaterials)(Close-bookformat)QuizII: 10%finalmark(SKKmaterials)(Close-bookformat)Finalexamination: 30% final mark (SKK 30%, Open-notes exam: Two A4 pages all you canwrite.)

39

BCHE4040AspectsofNeuroscience(2016-17,1stTerm)

DescriptionThis course will discuss the structure and components of nervous tissue inrelation to its functions. Aspects ofmetabolism special to the nerve and brainwill be examined. These include energy metabolism, water and electrolytebalance, exchanges between blood and brain, and between brain andcerebrospinal fluid, neurotransmitters and modulators, nutrition anddevelopmentof thenervous system,neuroendocrinologyand thebiochemistryofmentaldiseases.StudentsareadvisedtotakeBCHE2030orequivalentbeforetakingthiscourse.Contents/FundamentalConcepts1. Structureandfunctionofthecentralnervoussystem.2. Cellsofthenervoussystem3. Myelin;

a.Structureandfunctionb.Biosynthesisandgenesregulatingitsbiosynthesisc.Diseasesrelatedtomyelindysfunction,eg,multiplesclerosis

4. Biochemistry,physiologyandpharmacologyoftransportsystem:a.Blood-brain-CSFbarriersb.Cellular(Membrane)transportc.Axoplasmictransport

5. Ionchannels,potentialsandsignalconduction6. Metabolism:

a.Briefreviewofbrainmetabolismb.Someinheritedneurologicaldiseasesrelatedtometabolicdisordersc.Nutritionandbrainfunction

7. Biochemistry,physiologyandpharmacologyofsynaptictransmission:a.Classificationofneurotransmittersb.Biosynthesisoftransmittersandtheirregulationc.Terminationofneurotransmissiond.Propertiesandmolecularbiologyofreceptorse.2ndmessengersandsignaltransductionf.Modulationofsignaltransmission

8. Biochemistryandmolecularbiologyofneurologicalandpsychiatricdisordersa.Myastheniagravisb.Parkinson’sdiseasec.Alzheimer'sdiseased.Huntington’sdiseasee.Affectivedisordersandschizophrenia

40

LearningOutcomesAfterreadingthiscourse,studentsareexpectedbeableto:• Outlinethespecialfeaturesofthenervoussystemanditscomponents.• Describethefunctionsofthenervoussystembothatthecellularandmolecularlevels.

• Explainhowneuronscommunicatewitheachother incellularandmolecularterms.

• Explainthepathophysiologyofcertainneurologicalandneuropsychiatricdisorders,andthebiochemicalbasisoftreatingthem.

AssessmentSchemeMid-termExam45%FinalExam55% Recommended Readings • Neuroscience : exploring the brain / Mark F. Bear, Barry W. Connors,

Michael A. Paradiso (WL300 .B425 2007) • Neuroscience / edited by Dale Purves ... [et al.]. (WL102 .N487 2008) • Neuroscience at a glance / Roger A. Barker, Stephen Barasi ; and

neuropharmacology by Michael J. Neal. (WL102 .B326 2008) • Articles recommended by teachers.

Lecturers Prof. HY Edwin Chan MMW509B tel: 39434021 email: hyechan@cuhk.edu.hk Prof. KF Lau (Course Coordinator) SC 291 tel: 39431106 email: kflau@.cuhk.edu.hk

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Course Schedule BCHE4040 Aspects of Neuroscience (2016-17, Term 1) T3-4 (10:30 a.m. – 12:15 p.m.) MMW 710 and H4 (11:30 a.m. – 12:15 p.m.) SC L5

Wk. Date Hr. Teacher

1 Sept. 6 (T) 2 • Structure and function of the central nervous system Sept. 8 (H) 1 • Cells of the nervous system: neuron, Schwann cells and

astrocytes

2 Sept. 13 (T)

2 • Myelin

Sept. 15 (H)

1 • Biochemistry, physiology and pharmacology of transport system:

3 Sept. 20 (T)

2 a. Blood-brain-CSF barriers Prof. HY Chan

Sept. 22 (H)

1 b. Cellular (Membrane) transport

4 Sept. 27 (T)

2 c. Axoplasmic transport

Sept 29 (H)

1 • Ion channels, potentials and signal conduction

5 Oct. 4 (T) 2 Prof. KF Lau Oct. 6 (H) 1

6 Oct. 11 (T) 2 Mid-term Exam Prof. HY Chan Oct. 13(H) 1

7 Oct. 18 (T) 2 • Biochemistry, physiology and pharmacology of synaptic transmission

Oct. 20 (H) 1 • Classification of neurotransmitters 8 Oct. 25 (T) 2 • Biosynthesis of transmitters and their regulation Oct. 27 (H) 1 • Termination of neurotransmission

9 Nov. 1 (T) 2 • Properties and molecular biology of receptors Nov. 3 (H) 1 • Second messenger and signal transductioin Prof. KF Lau

10 Nov. 8 (T) 2 • Modulation of signal transmission Nov. 10

(H) 1 • Biochemistry and molecular biology of neurological and

11 Nov. 15#

(T) 2 • Psychiatric disorders

Nov. 17* (H)

0

12 Nov. 22 (T) 2 Nov. 24

(H) 1

13 Nov. 29 (T) Dec. 1 (H)

2 1

Total: 38 #Specialclassarrangement*Classsuspension(duetothe81stCongregationfortheConfermentofBachelor’sDegreesandMaster’sDegrees)

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BCHE4060 BASIC AND APPLIED IMMUNOLOGY 2016 – 2017, Term 1

Course Code: BCHE4060 (3 Units, First Term) Period: W3-4 (10:30 am - 12:15 pm);F3 (10:30 am - 11:15 am) Venue: SCL2 Medium of Instruction: English Objectives: This course aims at providing students with the essential concepts of basic and applied immunology. Topics to be covered include architecture and development of the immune system, antigens and antibodies, immunoglobulin-genes and -diversity, the major histocompatibility system and T cell receptors, tolerance and autoimmunity, and integration of immune responses. The effector mechanisms of the immune system will be introduced, with special emphasis on the role of innate and acquired immunity in health and disease. The relationship of immunology to clinical problems of infectious disease, immunodeficiency, hypersensitivity, transplantation and cancer will be discussed. The applications of immunology to modern medicine and biotechnology, including vaccination, monoclonal antibody production and antibody engineering will also be covered. Learning Outcomes: After completing the course, students should be able to:

• have an in-depth knowledge of the key features of the immune system; • comprehend the integrated nature of the immune system; • understand the molecular basis for generation of antibody diversity, immune response, self-tolerance

and disorders in the immune system; • appreciate the relevance of the immune system in our daily lives & the applications in biotechnology; • have developed competence in accessing & reviewing scientific literature, and critical appraisal of information. • Develop generic skills such as critical thinking, writing and lifelong learning skills. Lecturers: SK Kong MMWB Rm 609, Tel: 3943-6799, e-mail: skkong@cuhk.edu.hk Iris Pang SC 138, Tel: 3943-1393, email: irispang@cuhk.edu.hk Textbooks: (Some are reserved in the University Library) (QW504 .K83 2013) 1) Owen, Punt & Stranford (2013) Kuby Immunology (7th Ed) Macmillan

Kindt, Goldsby & Osborne (2007) Kuby Immunology (6th Ed) Freeman (W504.K53 2007) 2) Murphy (2012) Janeway’s Immunobiology (7th Ed) Garland Science. (QW504.J37 2012) 3) Abbas, Lichtman, and Pillai (2015) Cellular and Molecular Immunology (8th Ed) Saunders. (electronic resource at CUHK library: http://library.cuhk.edu.hk/record=b5884950~S15) 4) Doan, Melvold, Viselli & Waltenbaugh (2013) Immunology (2nd Ed) Lippincott Williams & Wilkins

(QW518.2.I55 2013) 5) Coico & Sunshine (2009) Immunology: A Short Course (6th Ed) Wiley-Blackwell (QW504.B35 2009) 6) Male, Brostoff, Roth & Roitt (2013) Immunology, (8th Ed) Mosby. (QW504.R65 2013)

43

e-Learning Materials: Biochemistry is a practical science. Learning the laboratory techniques and skills is an important element in the biochemistry curriculum. Videos (5-10 minutes each) for laboratory techniques (e.g. ELISA), skills for data presentation and self-study skills are available at http://www.bch.cuhk.edu.hk/learnbiochem/).

Assessment Scheme: There are 4 quizzes for this course (5% final mark each). The quizzes are part of the learning process. The questions are set to help you think about the important issues raised by the course materials and help you pin down the key items to be learned. Answers will be discussed when the quiz is marked. This feedback arrangement hopefully can correct the misunderstanding of the concept as early as possible. Quizzes: 4 (close-book) 20% final mark Mid-term examination (open-book examination): 30% final mark Term paper assignment 10% final mark Final examination (close-book): 40% final mark Posting of Course Contents and Announcements: Course materials including lectures slides, notes are all provided in the course Blackboard (https://elearn.cuhk.edu.hk/). Students can download the course materials from Blackboard using their student username and password. Feedback and Evaluation: Students are welcome to express feedbacks on course contents and learning experience through e-mail to the course teachers. A standard course evaluation questionnaire will be used to collect feedbacks from students at the end of the course. Course Outline: (* Quiz, 5% final mark each; Format: MCQ, T/F, Short Qs etc.) Week Date Hrs Topics Teacher 1 Sept 7 (Wed) 2 Overview of Our Immune System (2 L)

Prof. SK Kong

Sept 9 (Fri) 1 Nature of Antigens & Immunogens (1 L) 2 Sept 14 (Wed) 2 Lymphoid Tissues & Immunocytes (2 L)

Sept 16 (Fri) Holiday (Day after Mid-Autumn Festival) 3 Sept 21 (Wed) 2 Immunoglobulin Structure & Functions (2 L)

Sept 23 (Fri) 1 Antibody Diversity (1 L)

44

4 Sept 28 (Wed)* 2 Antibody Diversity (1 L) The Major Histocompatibility Complex (1 L)

(19 Lectures)

Sept 30 (Fri) 1 The Major Histocompatibility Complex (1 L) 5 Oct 5 (Wed) 2 T Cells & T Cell Receptors (2 L)

Oct 7 (Fri) 1 Integration of Immune Responses (1 L) 6 Oct 12 (Wed)* 2 Integration of Immune Responses (2 L)

Oct 14 (Fri) 1 Immunological Tolerance (1L) 7 Oct 19 (Wed) 2 Immunological Tolerance (1L)

Oct 21 (Fri) 1 Cytokines in Health & Diseases (1 L)

Dr. Iris Pang (17 Lectures)

8 Oct 26 (Wed) 2 Mid-term Examination Oct 28 (Fri) 1 Cytokines in Health & Diseases (1 L)

9 Nov 2 (Wed) 2 Host defense Part 1 & the Complement System (2 L)

Nov 4 (Fri) 1 Host Defense Part 2 (1 L) 10 Nov 9 (Wed)* 2 Emerging Infectious Diseases & Vaccine

Strategies (2 L) Nov 11 (Fri) 1 Allergy & Hypersensitivity Reactions (1 L)

11 Nov 16 (Wed) 2 Allergy & Hypersensitivity Reactions (2 L) Nov 18 (Fri) 1 Immunodeficiency & AIDS (1 L)

12 Nov 23 (Wed)* 2 Immunodeficiency & AIDS (2 L) Nov 25 (Fri) 1 Transplant Immunology (1 L)

13 Nov 30 (Wed) 2 Immunity to tumors (2 L) Dec 2 (Fri) 1 Immunity to tumors (1 L)

Tutorials: Tutorial 1 & 2 (optional): Time & Venue to be announced

Term Paper Assignment:

Write a term paper on the following (10% of the final grade): • To apply immunology concepts as tools for understanding real-world phenomena. • What you need to do is to use the knowledge of immunology to explain 2 pieces of experience in our daily life. Guidelines:

• Four students in one group will prepare 2 phenomena in 2 slides (in .ppt or .pptx format). • Below is one example showing how histamine from mast cells gives the Facial Flushing after drinking alcohol. •Ona3rdslide,putdownyourname&studentIDandaddonestatementforacademichonesty.

•Uploadyourtermpaper(3slides)toBlackboard(https://elearn.cuhk.edu.hk/).

•Deadline:5:00pm,2Nov2016(Latesubmission:markdeduction:1%ofthefinalgrade/day.)

Academic Honesty: Attention is drawn to University policy and regulations on honesty in academic work, and to the disciplinary guidelines and procedures applicable to breaches of such policy and regulations. Details may be found at http://www.cuhk.edu.hk/policy/academichonesty/. With each assignment, students will be required to submit a statement that they are aware of these policies, regulations, guidelines and procedures.

45

BCHE4070ManagementandAccreditationofBiochemicalLaboratory(2016-17,2ndTerm)

Time: Saturday,2:30pm-5:30pmClassroom: LT7,YasumotoInternationalAcademicPark(YIALT7)

MediumofInstruction: English

Unit: 3

MinimalPassingGrade:DCourseCoordination: Prof.SKKong,MMWBRm609,Tel:3943-6799,e-mail:skkong@cuhk.edu.hkCourseOutline:The aims of this course are to introduce basic concepts and adequate skills of laboratorymanagement,safetyandqualityassuranceinbiochemical laboratories. Specialtopicssuchas biochemical testing and manufacturing process, good laboratory practice, laboratoryaccreditation,genetictestingandexperimentalprotocolsandmethodvalidationetc,willbediscussed. The class will be taught with MSc students under different course code(BBMS6300)andassessmentscheme.TimeTable:

Week Date Lecture Teacher TitleoftheLecture

1 14Jan 1 Dr.KenYEUNG1 GoodLaboratoryPractice(GLP)

2 21Jan 2 Dr.KenYEUNG1 GoodLaboratoryPractice(GLP)

3 28Jan ----- Noclass(publicholiday)

4 4Feb 3 Dr.KenYEUNG1 GoodManufacturingPractice(GMP)

5 11Feb 4 Dr.AliceWONG2 IntellectualPropertyRightsRelevanttoBiotechnology

6 18Feb 5 Dr.AliceWONG2 TechnologyTransfer,LicensingandEnforcementofIPRs

7 25Feb ----- Noclass(studyweek)

8 4Mar TestI(2hours)Classroom:Rm702,MongManWaiBuilding

9 11Mar 6 Dr.JohnHO3

Whatisaccreditation?Howlaboratoryqualitymanagementsystemcanensurethequalityofresults?

46

10 18Mar 7 Dr.WilliamCHO4 ClinicalLaboratorySciencesandPractice

11 25Mar 8 Dr.WilliamCHO4 QualityAssuranceinClinicalLaboratory

12 1Apr 9 Dr.HenryCHEUNG QualityProgramsforForensicDNALaboratory

13 8Apr 10 Dr.BensonYEUNG5AccreditationProgramofAAALACInternationalforCareandUseofLaboratoryAnimals(1)

14 15Apr ----- Noclass(publicholiday)

15 22Apr 11 Dr.BensonYEUNG5AccreditationProgramofAAALACInternationalforCareandUseofLaboratoryAnimals(2)

16 29Apr CourseEvaluationTestII(2hours)Classroom:Rm705,MongManWaiBuilding

1 ChineseUniversityInstituteofBiotechnology,CUHK2 IntellectualPropertySpecialistatAlbertWai-KitChanIntellectualPropertyLimited3 HongKongAccreditationService,HKSAR4 QueenElizabethHospital5 ResearchandDevelopmentManageratVanwayPharmaceuticalHoldingsLtd.Assessments:Termpaper : 20%TestI : 30%(Lectures1-5)TestII : 50%(Lectures6-11)References:Toberecommendedbyindividualteachers

47

Termpapersguidelines:• WriteatermpaperonatopictaughtinBCHE4070.• Notmorethan4pagesincludingfigures,references,font-size10,single-linespacing.• Deadline:PleasesubmitthetermpaperviaCUeLearningSystemonorbefore11:55pm,

14May2017(Sunday).• Attention is drawn toUniversity policy and regulations on honesty in academicwork,

andtothedisciplinaryguidelines&proceduresapplicabletobreachesofsuchpolicy&regulations. Details may be found athttp://www.cuhk.edu.hk/policy/academichonesty/.

• AllassignmentsmustFIRSTbesubmittedtoVeriGuide(https://academic.veriguide.org/academic/login_CUHK.jspx)forcheckingofplagiarism.AVeriGuidereceiptwillbeissuedbytheVeriGuidesystemuponstudents'uploadingofthesoftcopyoftheassignmentonline.

• Please attach the SIGNED VeriGuide receipt (sign electronically or scan the signedhardcopy) on the first page of your assignment and submit it through CU eLearningSystem(https://elearn.cuhk.edu.hk)formarking.

• SubmissionthroughothermeansorassignmentwithoutVeriGuidereceiptwillnotbemarked.

• Termpaperssubmittedafterthedesignateddeadlinewillbepenalizedaccordingly;one-daydelaywillbepenalizedfor5%oftotalmarksofthetermpaper,two-daydelaywillbepenalizedfor10%ofthetotalmarks,andsoon.

• Generalorganization:Thefollowingsectionsshouldbeincludedinyourtermpaper.(a) Title:Giveaninformativeandconcisetitleforthecase.(b) Summary:Giveyourfindingsandasummaryofthecase.(c) Introduction:Givebackgroundinformationandrelatetheproblem(s)youfound

withthecurrentinformation.(d) Main body: Present your ideas in a systematic and concise manner. When

necessary,supplyfigures,diagramsandtableswithlegends.(e) Discussionandconclusion:Giveasummaryofyourideas,andprovidethoughtful

discussionabouttheimplicationsofyoursuggestions.(f) References:Makesureyouprovideaccuratecitationswiththefollowingformat.List

referencesalphabetically.Journal:YoshidaH,KawaneK,KoikeM,MoriY,UchiyamaY,NagataS.Phosphatidylserine-dependentengulfmentbymacrophagesofnucleifromerythroidprecursorcells.Nature,2005,437:754-8.

Testformat:• SectionA:Close-notestest(MCQ/T/F) SectionB:Open-notestest(Essay/shortquestions)• Students need toreturn answer sheet/book for Section A before they can answer

Section B open-notes testquestions. Studentscannotrequestto review their answersaftertheyhavehandedintheiranswersheet/bookofSectionA.ForSectionB,studentswill ONLYbe allowed to bring in one piece of A4 paper to the examination hall.Studentsmaywritedown/printoutinformationonbothsidesofthepaper.

B.Sc.AttributesTable

GraduateSkills Teaching&Learning Practice Assessment

Effectivecommunication(written,oral,interpersonal) ! ! !

Coreknowledgeliteracy ! ! !

48

Problemsolving ! ! !

Criticalthinkingandevaluation ! ! !

Workautonomously ! ! "

Workinteams(withclassmate,supervisor&RAetc) " " !

Creativityandinnovation ! ! !

Informationtechnology(IT)literacy ! ! !

Lifelonglearningskills ! ! !Ethical behaviours in social/professional/workenvironment

" ! !

Responsible,effectivecitizenship ! ! !

49

BCHE4080BiochemistryforForensicSciences

(2016-17,1stTerm)CourseDescriptionThis course aims at introducing various biochemical principles and techniques forforensicanalysistothestudents.Studentswilllearnhowtoobserve,collect,analyzeand evaluate evidence found at crime scenes. The first part addresses theidentificationofbiologicalfluidssuchasblood,semen,andsalivafromcrimescene.Theemphasiswillbeonthebiochemicaltechnologiesusedinthepresumptiveandconfirmatory tests. The second part coversDNA analysis using RFLP and PCR-STRtypingmethods,interpretationofDNAtypingresults,andcourtpresentationofsuchbiologicalevidence. MitochondrialDNAtypingandYchromosomeDNAtypingwillalso be discussed. It is anticipated that students will apply the biochemistryknowledgetheyhaveacquiredinjunioryearsinthecourse.LearningOutcomesAfterfinishingthecourse,studentswillbeableto:1)Developanunderstandingoftheconceptandtheoryofforensicanalysis.2)Understandtheanalyticaltechniquesinvolved.3)Discusstheapplicationofforensicsciencetocrimedetection.AssessmentSchemeMid-termexamination: 12October(Wednesday) 30%FinalExamination: 9December(Friday)**Tobeconfirmed** 70%LearningResources(1) Forensic Science: From the Crime Scene to the Crime Lab. Richard Saferstein.

Pearson2013[HV8073S214](2) ForensicScience: An Introduction. RichardSaferstein. PrenticeHall2011 [HV

8073S214](3) ForensicBiology.RichardLi,CRCpress,TaylorandFrancisGroup2008[QH313.5

F67L5](4) AdvancedTopicsinForensicDNATyping:Methodology.JohnM.Butler.Elsevier

AcademicPress2012[electronicresource](5) FundamentalsofForensicDNATyping.JohnM.Butler.AcademicPress2010(6) ForensicDNATyping:Biology,Technology,andGeneticsofSTRMarkers.JohnM.

Butler.AcademicPress2005[electronicresource]

50

CourseSchedule:Wednesday 2:30pm-4:15pm (Weeks1-6) ScienceCentre(SC)LG23Friday 6:30pm-9:30pm (Weeks8-13) Yasumoto InternationalAcademicPark(YIA)LT7Week Date Hour(s

) Topic Teacher

1 7Sep(Wed)

2 IntroductiontoForensicSciences KFLau

2 14Sep(Wed)

2 ForensicSerology&BloodAnalysis KFLau

3 21Sep(Wed)

2 DetectionofSaliva,Semen&OtherBodilyFluids

KFLau

4 28Sep(Wed)

2 ForensicToxicology&…. KFLau

5 5Oct(Wed)

0 Noclass

6 12Oct(Wed)

1 Mid-TermExamination KFLau

7 21Oct(Fri) 0 Classsuspendedduetotropicalcyclonewarningsignalno.8

8* 28Oct(Fri) 3 1. ForensicsciencesandCSI#2. DevelopmentofDNAtyping

methods,DNAextractionandDNAquantitation

3. ForensicgeneticsandRFLPtyping4. PCR-STRtyping5. Resultsinterpretationandforensic

issues6. EvaluationofDNAevidenceand

presentationofsuchevidenceatcourts

7. YchromosomeDNAtyping,mitochondrialDNAtypingandaspecialtopic

HenryCheung

9* 4Nov(Fri) 3

10* 11Nov(Fri)

3

11* 18Nov(Fri)

3

12* 25Nov(Fri)

3

13* 2Dec(Fri) 3

Total: 27 #Optionalastheteachingmaterialwillbesimilartolecturesinthefirstfourweeks.*Students in theM.Sc. programme (BBMS5120BiochemicalGenetics and ForensicSciences)willjointheclasstogetherinthelecture.Teachers:

51

ProfessorLAUKwokFaiSC29139431106 kflau@cuhk.edu.hkDr.HenryKYCheungAFacilityforPostingCourseAnnouncements:Course announcements and materials will be posted on the Blackboard coursewebsite. The powerpoint slides used will be posted on the Blackboard coursewebsitebeforethelecture.NOhardcopieswillbedistributedintheclass.

52

BCHE4090BiochemistryforSportandExercise(2016-17,Term2)

Coursecode: BCHE4090Coursetitle: BiochemistryforSportandExerciseVenue: Y.C.LiangHallG04Unit: 2Term: 2Period: 9:30–11:15,Friday Instructor: FHLoOffice: MMW507BEmail: lofaihang@cuhk.edu.hkTelephone: 39435019 CourseObjectives:This course aims at introducing various biochemical aspects of sport and physicalactivities to the students. The course is designed to cover some basic anatomy,energy utilization, cardiac functions, injury mechanisms, sports therapeuticprinciples, endocrine effects on physical activities, nutrition for sports, physicalactivities inextremeconditions,andphysicalactivities forthediseasedpopulation.The course welcomes students with basic knowledge in biological science, wherevariousin-classactivitiesareintegratedsoastoletthestudentsapply,analyze,andevaluate theacademicknowledge indaily life.Thetrainingofstudents’ creativity,question asking, critical thinking, goal-setting, self-learning, and decision makingskills will also be emphasized: students are encouraged to participate in the self-reflectiveactivities,workshop,andgroupactivities.Throughoutthelearningprocess,itisanticipatedthatthestudentswillnotonlyacquiretheacademicknowledge,theywill also consolidate their learning and acquire useful skills for both personal andprofessionalaspects.LearningOutcomes:• Master the fundamental knowledge of physiology and anatomy of the

skeletomuscularsystem;• Comprehendthebasicknowledgeoftheselectedtopicsofsportsbiochemistry;• Experience and apply the knowledge of sports biochemistry in every-day-life

scenarios;• Identify,analyze,andcommentontherelationshipbetweenbiochemistryand

physicalactivities;• Develop practical skills in goal-setting, problem-solving, team-work, and

communicationskills;• Gain learning methodologies that promote life-long learning in biochemistry

andotherrelateddisciplines.

53

Textbooks:• ACSM'sadvancedexercisephysiology(Farrelletal);[WE103.A832012]• ACSM'sguidelinesforexercisetestingandprescription(Thompsonetal);[WE103.A452010]• Biologicalpsychology(JamesWKalat);[WL102.K332013]• Essentialhaematology(Hoffbrand&Moss);[availableonline]• Exercisephysiology:nutrition,energy,andhumanperformance(McArdleetal);[QT260.M375

2010]• Managingsportsinjuries(ChristopherMNorris);[availableonline]• Marks'basicmedicalbiochemistry:aclinicalapproach(Liebermanetal);[availableonline]• Medicalbiochemistry(Baynes&Dominiczak);[availableonline]• Principlesofanatomy&physiology(Tortora&Derrickson);[QS4.T672012]• Principlesofathletictraining(Arnheim&Prentice);[QT260.A761995]• RobbinsandCotranpathologicbasisofdisease(Robbinsetal);[availableonline]• Sportsandexercisenutrition(McArdleetal);[TX361.A8M382013]• Wildernessmedicine(PaulSAuerbach);[availableonline]

CourseAssessment

Classwork 20%Mind-map* 10%In-classactivities** 10%Writtenassignment 30%Groupwrittenassignment+ 25%Individualwrittenassignment# 5%Quizandexam 50%Quiz 10%Finalexam(T/F,MCQ,andSQ) 40%Total 100%*Themind-mapofeachstudentisassessedbythewholeclassintermsofthecreativity;**AnyFIVEoftheclassactivitiesItoVIIaccountfor10%ofthetotalmarksofthecourse(2%each);+Groupwrittenassignmentrequireseachgrouptodiscussandreflectonthegroupactivityineitherweek13or14.Eachgroup,withatleastthreestudents,isrequiredtopresent1)whatdidyoulearnfromthecourse(5%),2)whatwasthemostinterestingacademicknowledgetoyou(5%),3)howdidyouintegratewhatyoulearntinthecoursetoanalyzethesituationpresentedtoyouduringthegroupactivity(5%),4)whatjudgmentdidyoumakeinthesituation(5%),and5)whatdidyouplantodointhesituation(5%)inthewrittenassignment;#Individualwrittenassignmentrequireseachstudenttoreflectonwhatdidtheylearnfromalltheclassactivities.

54

ScheduleWeek Date Contents

1 13Jan - Courseintroduction- Skeletalsystem

2 20Jan - Muscularandnervoussystem- ClassactivityI:bodyposture

27Jan - ChineseNewYear

3 3Feb

- Neuromuscularcontrol- Musclecontractionandsynthesis- Bonesynthesisandsofttissuebiochemistry- ClassactivityII:balancingthebody

4 10Feb- Energyutilizationatrestandduringphysical

activities- ClassactivityIII:estimationofbasalmetabolicrate

5 17Feb

- Haematology- Cardiacfunctionsforphysicalactivities- Cardiovascularsystemduringphysicalactivities- ClassactivitiesIV:bloodpressuremeasurement

6 24Feb

- Quiz:skeletomuscularsystem- Molecularmechanismofcellularinjuryandrecovery- Ischemia,inflammation,andspasm- Fatigueandrest

7 3Mar - Adaptationmechanismstoextremeenvironments- ClassactivityV:mind-mapdrawing

8 10Mar- Endocrineeffectonphysicalactivities- Biochemicalprinciplesoftraininganddoping- ClassactivityVI:howstressedyouare?

9 17Mar

- Nutritionforsports- Fatcellbiology- ClassactivityVII:measurementofbodyfat

composition

10 24Mar

- Physicalactivitiesinextremeconditions- Biochemicalprinciplesofsportstherapeutics- ClassactivityVIII:measurementofbloodO2

saturation

11 31Mar - Creativeworkshop- Critiqueonmind-map

12 7Apr - Groupactivity

12 14Apr - Easter13 21Apr - Groupactivity

Creativeself-reflectionSelf-reflective activity will be conducted in the form of mind-map drawing; eachstudent will assign a specific topic for him/herself during the preparation of themind-map,accordingtotheguidelinesprovidedinClassActivityV.Thestudentswillbriefly introduce the ideasof theirmaps to the classduring theCritiqueonmind-

55

map.Theassessmentcriterionofthemind-mapwillbebasedonthecreativityofthemind-map,whichwillbeassessedcollectivelybythewholeclassduringthecritique.GroupactivityforlearningintegrationGroupactivitywillbeheldattheendofthecourse;wherestudentswillbedividedintofourlargegroups;twogroupsoneachday(studentswillbefurtherdividedintosmall groups according to their plan and judgment). Before the group activity,studentswillberemindedtorevisethematerialscoveredbythecourseandtodoextraself-studyabout(wilderness)emergencyrescue.Duringeach45-minutegroupactivity,studentswillbebriefedwithacaseofdisaster:eachlargegroupwillactasthepatients;whiletheotherlargegroupwillactastherescuers.Therescuergroupwillhavetoapplytheirskillstocomprehendandanalyzethesituation,toapplytheirknowledgeandcreativity,andtomakejudgmenttoactasateam.Therescuergroupwill be allowed to debrief among themselves after the activity. The students willrotatetheirrolesoncetheactivityhasbeencompleted.Theactivitywilltakeplacewithintheclassroomandeachstudentwillhavetomakeuseoftheirimagination.AcademicHonestyAttentionisdrawntoUniversitypolicyandregulationsonhonestyinacademicwork,and to the disciplinary guidelines and procedures applicable to breaches of suchpolicy and regulations. Details may be found athttp://www.cuhk.edu.hk/policy/academichonesty/. For ALL written assignment,students will have to submit a statement that they are aware of these policies,regulations,guidelinesandprocedures.

56

BCHE4130MolecularEndocrinology

(2016-17,2ndTerm)This coursedescribes the chemical structuresandbiological functionsofhormones invertebrateswithemphasisbutnotfocusonlyonhumansubjects.Theorganizationandoperationofdifferenthypothalamus-pituitary-peripheralendocrineglandaxeswillbediscussed. Themolecular aspects of endocrine action through hormone secretion andinteractionswiththeirspecificreceptorsanddownstreamsignalingpathwaysintargetcells to elicit specific functions will be explored. Current advances in molecularendocrinologytechniqueswillalsobediscussed.Textbooks:Hadley,ME&Levine,JE(2007).Endocrinology,6thEd.UpperSaddleRiver,N.J.,500p.

(WK100.H172007;ULreserved)Molina,PE(2013).EndocrinePhysiology,4thEd.,McGrawHill

(http://accessmedicine.mhmedical.com/book.aspx?bookid=507)Nussey,SS&Whitehead,SA(2001).Endocrinology,AnIntegratedApproach,BIOS,

359p.http://www.ncbi.nlm.nih.gov/books/NBK22/(NCBITextbookOn-line)OutlineandTeachingSchedule:Time:T3-4(10:30amto12:15pm)andH6(1:30pmto2:15pm);Venue:SCL4Week

Date Topic(TextChaptersinHadleyandLevine,2007)

Teachers

1 Jan10(2h)Jan12(1h)

1.MechanismsofHormoneAction(1,2,3) KMChan(Coordinator)SC184Tel:3943-4420Email:kingchan@cuhk.edu.hk

2 Jan17(2h)Jan19(1h)

2.EndocrineMethodologiesandSignalTransductions(4)

3 Jan24(2h)Jan26(1h)

3.HypothalamicandPituitaryHormones(5,6,12)

4 Feb2(1h)Feb7(2h)

4.PosteriorPituitaryHormones&POMC(7,8)

5 Feb9(1h)Feb14(2h)

5.PancreaticHormonesandMetabolicRegulation(11)

6 Feb16(1h)Feb21(2h)

6.Hypothalamic-Pituitary-IGFAxisandGrowthControl(12)

7 Feb23(1h)Feb28(2h)

7.Hypothalamic-Pituitary-ThyroidAxis(13,14)

8 Mar2(1h)Mar7(2h)

8.Hypothalamic-Pituitary-GonadsAxis(17-19)

9 Mar9(1h)Mar14(2h)Mar16(1h)

9.Presentationonhormonetechniques$

10 Mar21(2h)Mar23(1h)

Studyweekande-learning:on-linevideosonhormonalcontrolofsugar,lipid,andcalciummetabolisms

11 Mar28(2h)Mar30(1h)

10.CalciumHomeostasisandBoneMetabolism(9)

FHLoMMW507BTel:3943-5019Email:lofaihang@cuhk.edu.hk

12 Apr6(1h)Apr11(2h)

11.Reproduction,SexHormonesandContraceptives

Apr13(1h)Apr18(2h)

12.Steroidhormonesandtheiractions(15-16)

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13 Apr20(1h)Apr25(2h)

13.Gastrointestinal(GI)Hormones(10)

Evaluations:1. $Presentation(group)onHormoneTechniques(15%):5%peer-assessment;

10%basedonoralpresentationandslidessubmittedasgroupproject.Pleaseincludeonemultiple-choicequestioninyourpresentation.

2. ^Assignment(25%coveringweek3-8):studentsshouldanswerany2questionspostedonlecturenotes,studentsarerequiredtowriteresearchproposalordesignexperimentstotesthypothesisordiscusscasestudy.Allstudentsareencouragedtoapplycriticalthinking,creativity,andproblem-solvingskillstosolvearesearchproblem.Warning:notwoassignmentscouldhavesimilarcontentsandthatwouldberegardedasplagiarism(DEADLINE:APRIL2,2016).

3. #Poster(group)Presentation(20%):coveringallendocrinologyresearchtopics(DEADLINE:May1,2015).

4. *FINALEXAMINATION(40%):coveringalltopicstaughtfromWeek1to13,datetobeconfirmed.

$OralPresentationonHormoneTechniques:studentsinagroupof3-4studentsshouldselectandpresentin10minutesaspecialandadvancedtechniquetodetecthormonesorstudytheeffectsofhormonesinanimals.Powerpointslides(<12)shouldbesubmittedonedayaheadofpresentation.Pleaseincludeonemultiple-choicequestioninyourpresentation.^Assignments:eachstudentshouldsubmitawrittenassignmentdescribingtheiroriginalapproachtosolvearesearchproblem.Intheassignment,1)aresearchproblemrelevanttothecourseshouldbeclearlyidentified;2)thehypothesisshouldbeclearlydefined;3)themethodfollowedbythehypothesisshouldbedescribed;4)howthedatashouldbeanalyzedinordertogetconclusionshouldbementioned;5)thereferenceofscientificinformationshouldbecited;6)studentfullnameandstudentIDshouldbeincluded.(Pagelimits:10;maxwordcounts:6,000)#Posterpresentation:studentsinagroupof2-3studentswouldselectaresearchpaperwithinlast5years(2011-16)andwriteanoverview(commentary)onthemajorfindingsofthepaper(s),rationalesandmeritofthestudy,anddiscusstheperspectiveorfutureexperimentsthatcanbeperformedtoextendthestudyandtheunderstandingofthesubjectmatter.Allpostersshouldbefinishedwithinonepage(maximumwordcountis2000,with3-4figuresortables)inapdffiletobeuploadedtoblackboardwiththesignedVeriGuidestatement(onpage2).Contentsoftheposterinclude[1]thetitleoftheposter,[2]studentnames(id),[3]fullname(s)ofthearticle(s)reviewed(withlinksanddownloadeddates),[4]abstracts,[5]keywords,[6]majorfindingswith2-3figuresortables,[5]critiques,[6]discussions(e.g.accuracyoftechniquesinvolved),[7]conclusions,[8]perspectives,and[9]references.*FinalExamination:thisisanopennoteexaminationandstudentsmaytakeonlyoneA4paperwiththeirownnotestofinishthisexamconsistsofmainlymultiplechoiceorshort-answerquestionsonconceptsofendocrinology.ExpectedlearningoutcomesAftercompletingthecourse,studentsshouldbeableto:1.Understandthehomeostaticcontrols in our body using the endocrine system; 2. Understand how polypeptide hormones and lipid soluble hormones act on their target cells to

activate various intracellular signaling pathways;

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3. Understand the concepts of hormonal control via feedback mechanisms; 4. Understand the etiology and diagnosis of diseases from hormone defects, such as gigantism and

Cushing Syndrome. By the end of the course, students will be able to appreciate and find information about the complexes of hormonal control of growth, metabolism, reproduction and development.

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BCHE4760ImmunologyandHaematologyLaboratory

(2016-17,Term1)Coursecode: BCHE4760Coursetitle: ImmunologyandHaematologylaboratoryVenue: Courseintroductionandexamination:LadyShawBuilding

LT6Oralpresentation:LeeShauKeeBuildingLT3

Practicalsessions:ScienceCentreRoom188/190Unit: 2Term 1Period: 2:30–6:15pm,ThursdayMaximum classsize:

30

CourseObjectives:This course is designed to focus on the practical work in the fields ofimmunologyandhaeamtology;wherestudentswillparticipate in fivepracticalsessionsspecializedinspecificthemesofimmunologyandhaeamtology,suchasinnateimmunity,humoral immunity,haematologicalspecimens,redbloodcells(RBCs), white blood cells (WBCs), and immunological assays. The practice of‘flippedclassroom’willbe implemented in this laboratorycourse: studentsarerequiredtodoself-studyofthee-learningcoursematerialsbeforehand;studentsareexpected toacquireadequateknowledge toattend the laboratory. Inotherwords, the laboratory is a place for the students to practise, to experience, tosolveproblems,and todiscuss.At theendofeachpractical session,discussiontime will be scheduled for the written assignment. In the meantime, there iscollaborationwith ILC to cover technical skills of scientific communication.Onthe other hand, important generic skills, such as team building skills, will becoveredby the course.The trainingof students’ self-learning,problem-solving,teamwork,andscientificcommunicationskillswillalsobeemphasized:studentsare encouraged to give presentations with topics in immunology they areinterested in; they will be fully supported and provided with the optimallearning environment to reflect on the topics they select. Throughout thelearning process, it is anticipated that the students will not only acquire theacademicknowledgeandlaboratoryskillsinimmunologyandhaematology,theywill also consolidate their learning and acquire other skills of self-learning,problem-solving, teamwork,andcommunication for futurecareerdevelopmentaswellasevery-day-lifeapplication.

60

LearningOutcomes:• Comprehend the core knowledge and relevant laboratory skills inimmunologyandhaematology;

• Develop generic skills in scientific communication and working in a teamthroughdiversetypesoflearningactivities;

• Gain learningmethodologies thatpromote life-long learning in immunology,haeamtology,andotherlifescience-relateddiscipline.

CourseAssessmentPerformance1&participation2

10%

Writtenassignment 25%Write-upform3,4Data5

15%10%

Presentation 25%Preparation 4%Coordinators 10%Demonstrators 5%Peers6 6%LabExam 40%Total 100%1Oneperformancemarkwillbeawardedtogroupsfinishingtheirpracticalworkbefore5:30pm;2TheparticipationmarkwillbedeductedforstudentswhoislatefororfailtoattendILCWorkshop;3Thewrite-upformofeachpracticalsessionaccountsfor3%ofthetotalmarksofthecourse;4Eachwrite-upformwillhavetobesubmittedontoBlackboardwithinoneweekofthecorrespondingpracticalsession;5Thedataofeachpracticalsessionaccountsfor2%ofthetotalmarksofthecourse;1%isallocatedfortheaccuracyoftherawdataand1%isforthescientificpresentationoftherawdata.6Fivemarkswillbeallocatedforthequalityofthepresentationsandamaximumof1markwillbeawardedtogoodteamsnominatedbypeers.

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StaffCorrespondence

Name Office Telephonenumber Emailaddress

Courselecturers KONG,SiuKai MMW609 3943-6799 skkong@cuhk.edu.hkPANG,Iris SC138 3943-1393 irispang@cuhk.edu.hkCoursecoordinators

KONG,Ada MMW525 3943-6255 adakong@cuhk.edu.hkLO,FaiHang MMW507B 3943-4359 lofaihang@cuhk.edu.hkDemonstrators CHENG,Lilian SC193 3943-6118 hiufucheng@yahoo.com.hkLAM,Thomas MMW610 3943-8034 thomaslam_521@yahoo.com.hkLAW,Iris MMW610 3943-8034 lokgi14@gmail.comWONG,Margaret MMW610 3943-8034 catbone_margaret_wong@yahoo.co

m.hkYEUNG,Jacky MMW610 3943-8034 yeungkawing223@yahoo.com.hkZHANG,Huawei SC193 3943-6118 zhw2508@gmail.comTextbooks:• Refer to lecture course (BCHE4060 Basic and Applied Immunology and

BCHE4030ClinicalBiochemistry)SupplementaryReferences:• PracticalImmunology(Hay&Westwood)

[availableonline];• LaboratoryHematologyPractice(Kottke-Marchant&Davis)

[availableonline];• Laboratory Diagnosis of Infectious Diseases: Essentials of Diagnostic

Microbiology(Engelkirk&Duben-Engelkirk)QW25.E54(2008);

• DiagnosticTechniquesinHematologicalMalignancies(ErberWN,ed)WH525.D53(2010);

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ScheduleWeek Date Experiment Venue Staff

1 8Sept

- LabCheck-In/studentconsultation

- Grouping/presentationtopicselection

LSBLT6 LFH

2 15Sept - Studyweek 3 22Sept - Studyweek

4 29Sept - AssayforInnateImmunity- AssayforHumoralImmunity(I)

SC188/190

Ada,Jacky,&others

5 6Oct - AssayforHumoralImmunity(II) SC188/190

Ada,Huawei,Lilian,&others

6 13Oct - Studyweek

7 20Oct - AssaysofHaematologicalSpecimens

SC188/190

Ada,Margaret,&others

8 27Oct - AssaysofRedBloodCells(RBCs) SC188/190

Ada,Thomas,&others

9 3Nov - ILCWorkshopILC

expertteam

ILC

10 10Nov - FlowCytometricAnalysisandImmunologicalAssay

SC188/190

Ada,Iris,&others

11 17Nov - UniversityCeremony 12 24Nov - Labexamination LSBLT6 All

13 1Dec

- GroupPresentationonImmunologicalTechniques

- Laboratorydiscussionandgroupsharing

LSKLT3 All

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BCHE4830MedicalBiochemistryLaboratory(2016-17,Term2)

Coursecode: BCHE4830Coursetitle: MedicalbiochemistrylaboratoryVenue: Classrooms:ScienceCentreLG23 Laboratory:ScienceCentreEastBlockRoom402Unit: 2Term: 2Period: 2:30–6:15pm,Monday CourseObjectives:Inthiscourse,studentswillbeintroducedtoaseriesofpracticalsessionsspecializedin representative themes, such as the analysis of diabetesmellitus, liver function,endocrine function, tumour marker, which supplements the knowledge acquiredfrom BCHE4030 Clinical Biochemistry/BCHE4130 Molecular Endocrinology andprovidesanexperiential learningopportunitiesof thepracticalskills. Inadditiontotheacademicknowledgeandlaboratorytechniques,otherlearningactivitiesarealsointegrated into thecourse toequipour students for theprofessionalanddaily lifescenarios theymay encounter in the future; the holistic training of students’self-learning,problem-solving,teamwork,andcommunicationskillswillbeemphasized:all students are required to work on self-reflection about the impact of medicalbiochemistryonoursociety.Thestudentswillparticipateclassactivity together toapply their knowledge in real life situations. Theywill alsobeencouraged to formgroupsandexplorespecifictopicsinterestingtothem,aswellastovisitsitesoutofthe campus. Throughout the diversified learning experiences in the course, it isanticipated that the students will not only acquire the academic knowledge andlaboratorytechniquesinmedicalbiochemistry,butalsodevelopusefulgenericskills,apply their learning in real life scenarios, and equip themselves for their furtherstudiesandcareerdevelopment.LearningOutcomes:• Comprehend and apply the basic knowledge and laboratory skills in medical

biochemistry;• Recognize,analyze,andcommentontheimportance,aswellasthescientificand

socialissuesofmedicalbiochemistryinclinicalpractice;• Develop practical skills in goal-setting, self-learning, critical thinking, problem-

solving, team-work, and communication skills through the various types oflearningactivitiesofferedbythecourse;

• Gain learning methodologies that promote life-long learning in medicalbiochemistryandotherlifescience-relateddiscipline.

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Textbook:• ClinicalChemistry(WilliamJMarshall&StephenKBangert)

(availableonline)• MedicalBiochemistry(JohnWBaynes&MarekHDominiczak)

(availableonline)• EssentialHaematology(VictorHoffbrand)

(availableonline)

References:• Tietztextbookofclinicalchemistryandmoleculardiagnostics(CarlA.Burtisetal)

(availableonline); ScheduleWeek Date Activity/Experiment Venue Staff

1 9Jan - LabCheck-In/Grouping- Courseintroduction SCLG23 All

2 16Jan - Add/Dropperiod

3 23Jan - PartOne(a&b) SCE402 AnitaandDemonstrators

4 30Feb - ChineseNewYear 5 6Feb - Studyweek 6 13Feb - PartTwo(a&b)

SCE402 AnitaandDemonstrators7 20Feb - PartTwo(b&c)

8 27Feb - Studyweek

9 6Mar - PartThree(a&b) SCE402 AnitaandDemonstrators

10 13Mar - Workshop:problem-solving SCLG23 LFH11 20Mar - Studyweek

1227Mar - Park4

SCE402 AnitaandDemonstrators28Mar - Park4follow-up

13 3Apr - Studyweek

14 10Apr - Labexam TBC LFH15 17Apr - EasterHoliday

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StaffCorrespondence

Name Office Telephonenumber Emailaddress

Courselecturers ProfCHAN,KingMing SC184 3943-4420 kingchan@cuhk.edu.hkProfKONG,SiuKai MMW609 3943-6799 skkong@cuhk.edu.hkCoursecoordinators LO,FaiHang MMW507B 3943-5019 lofaihang@cuhk.edu.hkYIU,Anita SCE412A 3943-6881 anita-garcia@cuhk.edu.hk

CourseAssessmentPerformance&participation 10%Data 20%Rawdata1 10%Presentabledata2 10%Writtenassignment 30%Write-upform3 20%Reflectivejournal 10%Labexam 40%Total 100%1.Studentswillhavetoprovidetheirrawattheendofeachpracticalsession;therawdataofeachpracticalsessionaccountsfor2%.Groupswithdeviateddatareceivenomark.2.Thepresentabledataofeachpracticalsessionaccountsfor2%.3.Thewrite-upformofeachpracticalsessionaccountsfor4%.

PresentabledataEachgroupwillhavetoworktogethertopresenttheirrawdatainanappropriatemanner.Thepresentabledatawillthenbeprintedoutandsubmitwiththeirwrite-upform.ReflectivejournalEachstudentwillhavetowriteanindividualreflectivejournaltitled‘TheImpactofMedicalBiochemistryontheSociety likeHongKong’. Inthereflective journal, thestudent has to demonstrate his/her understanding of the topics by analyzing andcommending the selected fromdifferent perspectives.The reflective journal (lessthan 2,000 words) willhave to be submitted ontoBlackboard with aVeriGuidestatementbythelastdayon22April(Saturday).FlippedclassroomIn this laboratory course, students are requested to view the pre-lab videosbroadcasted on Blackboard and get thoroughly prepared before they attend thelaboratory.When the laboratory course starts, the demonstrator in charge of thedaywillprovidebasicguidanceoftheexperiment(s)involved;thenthestudentsareexpected towork independently, withminimal interference by the demonstrator

66

team,wherefrom5:30to6:15pmisadedicateddiscussionsessionheldatSC297orMMW622conferencerooms.Week Date Discussionvenue4 29Sept MMW6225 6Oct SC2977 20Oct SC2978 27Oct SC29710 10Nov SC297

Multi-dimensionaldevelopmentmodules(optional)ModuleA:sitevisits(Bonus:5marks)Studentsmaywishtoformgroupsandvisittheapprovedsitesaccordingtothecourserequirement.TheproofofthevisitwillhavetobesubmittedontoBlackboardby7May(Sunday).Approvedsites#EducationalactivitiesorganizedbyCancerFund

Website:http://www.cancer-fund.org/en/howwehelp_download.html$EducationalactivitiesorganizedbyHongKongCollegeofCardiology

Website:http://www.hkcchk.com/index.php%EducationalactivitiesorganizedbyStrokeFund

Website:http://www.strokefund.org/eng/joinus_part3.php&HongKongMuseumofMedicalSciences(recommended)

Website:http://www.hkmms.org.hk/English/main.htm'HongKongScienceMuseum

Website:http://hk.science.museum/eindex.phpOthersiteswiththeapprovalofthecoursecoordinatorModuleB:Informationtechnologyandtransferableskillstraining(Bonus:5marks)Studentsmaywishtoparticipatethetrainingcourse/workshopofferedbytheUniversityLibraryorInformationTechnologyServicesCentrerelatedtoITskills,suchasinformationsearch,computersoftwaretraining,etc.OthereventsofferedbytheCareerPlanningandDevelopmentofCUHK,orpublicseminarsofferedbyotherorganizationstopromotetransferableskillsinspecifictopicswillalsobeaccepted.TheproofofparticipationwillhavetobesubmittedontoBlackboardwithaby7May(Sunday).Usefulwebsites#CUHKLibraryWorkshopWebsite:http://www.lib.cuhk.edu.hk/en/learning/workshops/$CareerPlanningandDevelopmentCentre

Website:http://cpdc.osa.cuhk.edu.hk/%HongKongPublicLibrary

Website:http://www.hkpl.gov.hk/

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&HongKongScienceParkCorporationWebsite:https://www.hkstp.org/hkstp_web/en/Events-calendar/

'ProductivityTrainingInstituteWebsite:https://www.hktrainingonline.com/

OthertrainingwiththeapprovalofthecoursecoordinatorModuleC:IndependentLearningCentreLanguageImprovementWorkshop(Bonus:5marks)StudentswillhavetoparticipatetheworkshopofferedbyIndependentLearningCentrerelatedtoscientificwritingorotherrelatedgenericandcommunicationskillsapprovedbythecoursecoordinators.TheproofofparticipationwillhavetobesubmittedontoBlackboardwithaby7May(Sunday).ModuleD:VideoPresentationon‘howtoimprovethecommunicationandsocialskillsofshyandintrovertedstudents’(Bonus:5marks)Studentswillhavetoformagroupoffivestudentsandproduceavideopresentationtitled‘howtoimprovethecommunicationandsocialskillsofshyandintrovertedstudents?’.ThevideoshouldNOTexceed5minutesandthefacesofallthepresentershavetobeclearlyseenforidentification.Eachstudentisrequiredtohaveevenshareoftheairtimeofthevideo(~1minuteperstudent).ThevideowillhavetobesubmittedontoBlackboardby7May(Sunday).

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BCHE4901SeniorExperimentalProjectI(2016-17)

CourseDescriptionIn this course, students carry out an independent laboratory research projecteitherprovidedby thesupervisoror jointlydecidedwith thestudent.To fulfillthe course requirement, students are required to discuss their progress withtheirsupervisorsregularlyandsubmitaresearchproposal.LearningOutcomeStudentstakenthiscourseareexpectedtodevelopthefollowingabilities:1.Integratetheknowledgeandskillslearntpreviouslyinothercourses2.Surveyscientificliteraturerelevanttotheirresearchproject3.Readscientificliteraturecriticallytoidentifythescientificquestionsorproblems4.Designindependentresearchprojecttoaddressthescientificquestions5.Acquirehands-onresearchtechniquesinconductingexperiments6.Developproblem-solvingabilities7.Improveskillsinwritingscientificproposal8.DevelopasenseofresponsibilityandtermspiritCourseSyllabusThe students should take the initiative to discuss with their supervisors andcompromiseatopicfortheindependentresearchproject.Thestudentsshouldfirstsurveythebackgroundoftheirresearchprojectandidentifythescientificquestionstheywanttoaddress.Thestudentsthendesignandperformtheexperimentsforthisprojectundertheguidanceofthesupervisorsandsubmitaresearchproposalinthetermend.AssessmentSchemeEssays 40%Other 60%RequiredandRecommendedReadingsRelevantreferencematerialswillbeprovidedbythesupervisor.EnrollmentRequirementsPrerequisite:BCHE3090

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BCHE4902SeniorExperimentalProjectII(2016-17)

CourseDescriptionStudentsarerequiredtodiscusstheirprogresswiththeirsupervisor(s)regularlyandsubmitaprogressreportattheendoftheterm.ForstudentwhowillnotbeenrolledinBCHE4903,afinalreportintheformofamanuscriptandanoralpresentationareassessedattheendoftermforperformance.LearningOutcomeStudentstakenthiscourseareexpectedtodevelopthefollowingabilities:1.Integratetheknowledgeandskillslearntpreviouslyinothercourses2.Surveyscientificliteraturerelevanttotheirresearchproject3.Readscientificliteraturecriticallytoidentifythescientificquestionsorproblems4.Designindependentresearchprojecttoaddressthescientificquestions5.Acquirehands-onresearchtechniquesinconductingexperiments6.Developproblem-solvingabilities7.Developdataanalysisandinterpretationskillsinlogicalandscientificways8.Improveskillsinscientificwritingandoralpresentation9.DevelopasenseofresponsibilityandtermspiritCourseSyllabusThestudentsshouldcontinuetoworkontheresearchprojectfromBCHE4901.Studentsshoulddiscusstheprogresswiththeirsupervisorsandsubmitaprogressreportinthetermend.IfthestudentswillnotcontinuetoworkontheresearchprojectinBCHE4903,theyshouldsubmitafinalreportintheformofmanuscriptandpresentorallyinthetermend.AssessmentSchemeEssays 40% Other 60% RequiredandRecommendedReadingsRelevantreferencematerialswillbeprovidedbythesupervisor.EnrollmentRequirementsPre-requisite:BCHE3090

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BCHE4903SeniorExperimentalProjectIII(2016-17)

CourseDescriptionInthiscourse,studentscontinuetoworkonandfinishuptheresearchprojectfromBCHE4902.Relevantdataanalysisandconclusionformulationareperformedafterdiscussionwithsupervisors.Afinalreportintheformofamanuscriptandanoralpresentationareassessedattheendoftermforperformance.LearningOutcomeStudentstakenthiscourseareexpectedtodevelopthefollowingabilities:1.Integratetheknowledgeandskillslearntpreviouslyinothercourses2.Surveyscientificliteraturerelevanttotheirresearchproject3.Readscientificliteraturecriticallytoidentifythescientificquestionsorproblems4.Designindependentresearchprojecttoaddressthescientificquestions5.Acquirehands-onresearchtechniquesinconductingexperiments6.Developproblem-solvingabilities7.Developdataanalysisandinterpretationskillsinalogicalandscientificways8.Improveskillsinscientificwritingandoralpresentation9.DevelopasenseofresponsibilityandtermspiritCourseSyllabusThestudentsshouldcontinuetoworkontheresearchprojectfromBCHE4902.Studentsshoulddiscusstheprogresswiththeirsupervisorsandsubmitafinalreportintheformofmanuscriptandpresentorallyinthetermend.AssessmentSchemeEssays 40% Presentation 40% Other 20% RequiredandRecommendedReadingsRelevantreferencematerialswillbeprovidedbythesupervisor.EnrollmentRequirementsPre-requisite(s): BCHE3090&BCHE4902.

- End–201708version