(hyaluronic acidHA)

(anti-adhesion)

(nanometersilica

powder, NSP)/

(Oligo-chitosan)

(Fe2O3 magnetic nanobeads, MAG)

(0, 50, 500,1000 ppm)

50 nm100 nm

(L929 cell line)

()

(Fe2O3 magnetic nanobeads, MAG)

1. TEM (Transmission Electron Microscope) (1)(125ppm)25-50 nm ( 1A,

1B)(MAG + Oligo chitoan)(1%)100nm

2. AFM(Atomic Force Microscopy) ( 2)

(MAG)(Oligo chitosan)(SMF)MAG(SMF, static magnetic field, 180mT )

3. () (3)(OM)

OMHA(NSP)

6.(MTT assay) (6)

1.2.3.

NSP

Sufiyarov I.F. Experimental validation for the use of a film on the basisof modified hyaluronic acid for prevention of postoperative peritoneal adhesions. Bull Exp Biol Med. 2007 Aug;144(2):269-71.

Cohen Z. et al. Prevention of postoperative abdominal adhesions by a novel, glycerol/sodium hyaluronate/carboxymethylcellulose-based bioresorbable membrane: a prospective, randomized, evaluator-blinded multicenter study.Dis Colon Rectum. 2005 Jun;48(6):1130-9.

1.2.3.4. AFM (atomic force microscopy)5. FESEM (field-emission scanning electron microscope) 6. Contact Angle System 7. ELISA (enzyme-linked immunosobent assay) reader 8. TEM( transmission Electron Microscope )

4. FESEM (1) L929 ( 4, 5)

()( 4, 250X ; 5, 1000X )

1

2

3

4 5

6