95026Lin_yuan_yun

1-1

1-2

1-3

1-4

2-1 HA

2-1-1

2-1-2

2-1-3

2-2 Oligo chitosan

2-2-1

2-2-2

2-2-3

2-3NSP

2-3-1

2-3-2

2-4 MAG: Fe2O3 naonparticle (FluidMAG-CT)

2-4-1

2-4-2

3-1

3-2

4-1 Oligo chitosan

4-2 OM

4-3 (HA)(OM)

4-4

4-4-1 (FESEM)

4-4-2

4-4-3 AFM

4-5

4-5-1 /

4-5-2

4-5-3 MTT test

5-1

5-1-1 TEM (Transmission Electron Microscope)

5-1-2 AFM(Atomic Force Microscopy)

5-1-3 NSP()

5-2

5-2-1

5-2-2

5-2-3 FESEM(1)

5-2-4 (MTT assay)

1-1

(hyaluronic acidHA

(anti-adhesion)

(nanometer silica powderNSP)

(MAG)

1-2

(Oligo chitosanMAG OM)

(NSP)

NSP

1-3

(HA)(NSP)

1-4

1.(MAG)(Oligo chitosan)

2.

3.NSP HA ?

2-1 HA

2-1-1

(Hyaluronic Acid HA) 1

-N-

Dalton

2-1-2

Hyaluronic Acids

Meyer

(:)

2-1-3

100

(Osteoarthritis)

1

(Synovial fluid)

(Anti-Adhesion)

93%

55 - 100%

2-2 Oligo chitosan

2-2-1

Oligo

Chitosan(2)

(Chitin)(Chitosan

)

1811

1980

1997

2-2-2

(Chitin)

(HCl)

(NaOH)

(Chitin)

(Chitosan)

2-2-3

2-3 NSP

2-3-1

2

(320nm)

NSPPolyamine

NSP(3)

2-3-2

NSP 100nmNSP

( 4)

3 NSP

2-4 MAG: Fe2O3 naonparticle (FluidMAG-CT)

2-4-1

Ultra-small Super Paramagnetic Iron Oxide

USPIO

12.5nm

USPIO

USPIO

USPIO

MRI

MRI

4 (NSP)

USPIO

USPIO6nmPCR

80 210-2

1 610-3

2-4-2

5

3-1

3-2

(MAG)(NSP)

4-1 Oligo chitosan

Oligo chitosanMAG( 6)

Oligo chitosanMAG

30 ddH20

35 OM

OM

4-2 OM

OMNSP 15mm

6 MAG

38

OM NSP 30

30 38

2~3

500l

1.OM250lNSP 0.9%250l

2.OM50lNSP 0.9%450l

3.OM25lNSP 0.9%475l

4.OM

5.Oligo chitosan

(OM)NSP

1.357ppm

2.100ppm

3.50ppm

4-3 (HA)(OM)

OMNSPHA

15mm 38

0.5 HA0.1

1HA2OM3NSP 30

38 2~3

1500l

1.HA1425l+ NSP1%75l+OM80l



4.HA1425l+ NSP1%75l

5.HA1500l

6.Control TCP ()

(OM)

1.500 ppm

2.100ppm

3.50ppm

4-4

4-4-1 (FESEM)

FESEM(Field Emulsion Scanning Electron Microscope)

(FESEM)

1.0 nm(15 kV)

2.2 nm(1 kV) ( 0.5 kV )

30SEM

4-4-2

/

-

/

CCD

PLGA Static contact angle

meter

2

4-4-3 AFM

AFMAtomic force microscope 7

STM

Z

10-6 10-10NNewton AFM

AFM 1

AFM 2 AFM

AFM 50nm

10-9m

AFM 3 AFM

24

4-5

4-5-1 /

L929 NCTC Clone 929 10 LDMEM

24 24-well 375

CO2 MTT test

4-5-2

4-5-3 MTT test

MTT3-(4,5)-dimethylthiahiazo

7 AFM

(-z-y1)-3,5-di- phenytetrazoliumromide, MTT

MTT

Ctetrazolium

formazanformazan

MTTformazan50%

N,N-20%pH 4.7MTT

490 nmODDMSO

MTT

1. MTT

2. DMSO

3. ELISA reader

1. MTT 20ul 3

2.

3. 150ul DMSO

4. 525nm ELISA reader

5-1

5-1-1TEM (Transmission Electron Microscope)

125ppm

10nm

MAG + Oligo chitoan 1%

100nm

100nm

100nm 100nm

50nm

5-1-2 AFM(Atomic Force Microscopy)

(MAG)(Oligo chitosan)

(SMF) MAG

(SMF, static magnetic field, 180mT )

5-1-3 NSP()

NSP

NSP

NSP(OM-NSP)OM-NSP

OM NSPOM-NSP

OM

NSPNSPHA

HA HA - NSP

HA-NSP-OM50 HA-NSP-OM 500

HA-NSP-OM 1000 HA-NSP-OM 1000

100x100x

100x100x

100x 100x

5-2

5-2-1

L929 ,

5-2-2

1(100X)

HA HA-NSP


HA-NSP-OM 1000

5-2-3 FESEM(1)

L929

FESEM

NSPNSP

250x

HA HA-NSP

HA-NSP-OM 50 HA -NSP-OM 500

HA-NSP-OM 1000

1000x

NSPHA

OMNSP

HA HA-NSP


HA-NSP-OM 1000

5-2-4 (MTT assay)

(tcp)

NSP

NSP

HA

NSP

NSP-OM 50OM-NSP

HA(* P

Sufiyarov I.F. Experimental validation for the use of a film on the basis

of modified hyaluronic acid for prevention of postoperative peritoneal

adhesions. Bull Exp Biol Med. 2007 Aug;144(2):269-71.

Cohen Z. et al. Prevention of postoperative abdominal adhesions by a novel,

glycerol/sodium hyaluronate/carboxymethylcellulose-based bioresorbable

membrane: a prospective, randomized, evaluator-blinded multicenter

study.

Dis Colon Rectum. 2005 Jun;48(6):1130-9.

1 HA /

2

http://www.fn.pu.edu.tw/epaper/27/27-2_1.htm

3 NSP

4

5 Chemicell.com, Germany.

6 MAG Chemicell.com, Germany

7 AFM

http://elearning.stut.edu.tw/caster/3/no6/6-2.htm

NSP () .

ppt

-

e-mail

2009.2

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95026Lin_yuan_yun