298 PATENT ABSTRACTS

Hybrid cells transformed or transformable by a cloned DNA sequence containing the sequence coding for a determined polypeptide, for ex- ample an immunogene polypeptide having vac- cination properties to hepatitis B. They are characterized in that they contain on one hand at least a portion of the genotype of a primary cell lineage which are naturally favorable to the ex- pression of said DNA sequence and on the other hand a genetic marker which enable them to grow in a selective medium or containing an ac- tive principle which is normally lethal for the cells from which the hybrid is issued, but unac- tivable by the polypeptide expressed by said genetic marker.

8503947

T - C E L L R E C E P T O R - S P E C I F I C F O R A N T I G E N P O L Y P E P T I D E S

A N D R E L A T E D P O L Y N U C L E O T I D E S

Mark M DAVIS, Stephen M HEDRICK as- signed to THE BOARD OF TRUSTEES OF THE LELAND STANFORD JR U

Olignnucleotide sequences are provided coding for T-cell-specific antigen receptors or fragments thereof. The oligonucleotide sequences can be used as probes for detecting helper and cytotoxic T-cells, preparing and isolating DNA sequences encoding for the receptor polypeptide, and in constructions for expression of receptor poly- peptides or fragments thereof. In addition, pro- cessing signals from the receptor subunits can be employed in conjuction with modified wild type oligonucleotide sequences or non-wild type olignucleotide sequences.

8 ~ 3 ~ 9

H O S T S A N D M E T H O D S F O R P R O D U C I N G R E C O M B I N A N T P R O D U C T S IN H I G H Y I E L D S

Alfred L GOLDBERG, Stephen A GOFF, Lawrence P CASSON assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Host organisms and methods for producing recombinant products in high yields. More par- ticularly, the present invention relates to cell strains carrying specific mutations within their DNA sequences which cause the cells to exhibit a reduced capacity for degrading foreign products due to the reduced expression of cellular pro-

teases and to the use of these strains to produce increased yields of genetically engineered foreign proteins, polypeptides and other products. The methods disclosed in this invention advan- tageously permit the production, in high yields, of foreign recombinant proteins, polypeptides or other products in hosts which do not usually pro- duce such products.

8504187

P R O C E S S F O R P R E P A R I N G 5 ' - G U A N Y L I C A C I D

Tatsuro FUJIO, Akihiko MARUYAMA, Tat- sunari NISHI, Akio OZAKI, Seiga ITO, Atsuko OZAKI, 6-29-1, Sagamidai, Sagamihara-shi, Kanagawa 228, Japan assigned to KYOWA HAKKO KOGYO CO LTD;

GMP can be prepared in a good yield by con- verting XMP, ammonia and/or L-glutamine in an aqueous solution using a culture product, cells or their treated product of E. coli having GMP synthetase activity and the ability of con- verting AMP to ATP in the presence of an energy source other than phosphorus oxides, in the pre- sence of the energy source. GMP can also be pre- pared in a good yield by converting XMP, ammonia and/or L-glutamine to GMP in an aqueous medium in the presence of ATP using a culture product, cells or their treated product of transformant obtained by using a recombinant DNA ofa DNA fragment containing GMP syn- thetase gene and a vector DNA fragment. The process of using transformant having ATP- reproducing ability is extremely advantageous.

8504188

R E C O M B I N A N T D N A M O L E C U L E S

Ashley Roger DUNN, Nicholas Martin GOUGH, Donald METCALF, 81 Panoramic Road, North Balwyn, VIC, Australia assigned to RESEARCH CORPORATION

DNA sequence coding for a mammalian granulocyte macrophage colony stimulating fac- tor (GM-CSF), a method of obtaining same, vec- tors and hosts harboring same. The sequence is useful as a probe for identifying related sequences, selecting GM-CSF encoding mRNA from a mixture of mRNAs containing same, and a source of GM-CSF DNA for expression in an appropriate expression vector. The GM-CSF