69528-101 m1000 Service Training Guidegss.pmh.com.br:9010/moleculardiagnostics_cd/69528101.pdf · Page 2 m1000™ Service Training Guide 69528-101—September 2003 Proprietary Information

Service TrainingGuide

Abbott LaboratoriesAbbott Park, IL 6006469528-101—September 2003

©2003 Abbott Laboratoriesm1000 is a Trademark of Abbott

Laboratories

Page 2m1000™ Service Training Guide69528-101—September 2003

Proprietary InformationConfidential Material

Not for Customer Distribution

Revision HistoryDocument Control Number(s) Revision Date Section(s) Revised Pages Revised and Added

69528-101 September 2003 Original Issue Original Issue




StatementsProprietary StatementCopyright 2003, Abbott Laboratories Diagnostics Division. All rights reserved.

the information, documents, and related graphics published herein (the “Information”) are the sole property of Abbott Laboratories. Permission to use the Information is granted, provided that:the copyright notice appears on all copiesuse of the Information is for the operation of Abbott products by Abbott trained personnel or informational use only;the Information is not modified in any way; andno graphics are used separate from accompanying text.

Each person assumes full responsibility and all risks arising from use of the Information. The Information is presented “as is” andmay include technical inaccuracies or typographical errors. Abbott Laboratories reserves the right to make additions, deletions, or modifications to the Information at any time without prior notification.This guide was developed and produced by Worldwide Customer Service and Medical Writing in Irving, TX.

Trademark StatementsAll Abbott Laboratories product names and trademarks are owned by or licensed to Abbott Laboratories, its subsidiaries oraffiliates. No use of any Abbott trademark, trade name, trade dress, or product name may be made without the prior written authorization of Abbott Laboratories, except to identify the product or services of Abbott Laboratories. All other trademarks, brands, product names, and trade names are the property of theirrespective companies. All rights reserved. Except as permitted above, no license or right, express or implied, is granted to any person under any patent, trademark, or other proprietary right of Abbott Laboratories.m1000 is a trademark of Abbott Laboratories.




Disclaimers

Pictorial DisclaimerAll samples (printouts, graphics, displays, screens, etc.) are for information and illustration purposes only, and shall not be used for clinical or maintenance evaluations. Data shown in sample printouts and screens do not reflect actual patient names or test results.

Master Table of Contents/Index DisclaimerIncremental manual updates may cause the Master Table of Contents or Master Index page numbering to change.

Field Service Manuals DisclaimerInformation contained in this document was developed for use by trained Abbott Laboratories Field Service personnel, or by other persons knowledgeable or experienced with operation and service of the m1000. The information should be used under direct supervision and cooperation with Abbott Laboratories technical sales and service representatives.

In no event shall Abbott Laboratories or its affiliates be liable for any damages or losses incurred in connection with, or arising from use of information in this document by persons not fully trained by Abbott Laboratories as technical sales and service representatives. This limitation shall not apply to those persons knowledgeable or experienced with operation and service of the m1000 system, who use this information under direct supervision of Abbott Laboratories technical sales and service representatives.




Table of ContentsPage6 Training Objectives7-10 m1000 Product Safety11-13 Instrument Overview14-15 Worktable16-19 Liquid Handling Arm (LiHa)20-23 Robotic Manipulator Arm (RoMa) 24-28 PosID (Positive Identification)29-31 1 ml Subsystem32-33 SMIO/SMIO-SAFY34 Sensors35 Fast Wash (FaWa) Pump Assembly36-37 OPTIBO38 Power Supply39-40 Liquid System41 Addresses42 System Communication43-46 Technical Data and Requirements47-48 Cabinet and Instrument Installation49-89 Software Installation and Information90-97 S&S – System Devices/LiHa98-106 S&S – System Devices/LiHa/Information

Page107 S&S – System Devices/RoMa/Pages (Tabs)108-109 S&S – System Devices/RoMa/Contents Page110-115 S&S – System Devices/RoMa/Setup Page116-117 Liquid System – DiTi118-125 Liquid System – DiTi/Lower DiTi Eject Test126-127 Liquid System – FaWa128-131 Liquid System/FaWa Pump Test

Configuration132 Options Menu133-135 Options/Access Status Options136-141 Options/Incubation Device142-143 Options/SMIO for NAT144-145 SMIO Panel146-147 Options/SMIO for NAT148 Labeling149-150 NAT Software Installation and Registration151-156 Calibration157 Move Plate Test158-175 Troubleshooting176-177 Preventative Maintenance190-192 CAN-BUS Resistance Test




Training ObjectivesUpon completion of the m1000 training class, the FSR should possess the following skills, abilities, and knowledge:

Perform pre-site inspection and installation of the m1000Understand basic theory of operation, power, wiring, fluidics, and sample progression of the systemLocate and describe the function of various subassemblies and partsPerform field service maintenance proceduresTroubleshoot the system in a timely manner using available service tools and manualsPossess ability to align and adjust all robotic devicesAchieve familiarity with Setup & Service (S&S) softwareAchieve familiarity with NAT software




m1000 Product Safety

Instrument-related Hazards and Safety MeasuresESD

Observe precautions for handling electrostatic discharge sensitive devices.Wear a wrist ground strap.Discharge static electricity from your body before touching the instrument.

Pinch PointThe instrument requires accurate positioning of system modules, and carriers and racks on worktables. While working with the instrument, it could be necessary to run tests with the instrument’s safety panels open.Keep away from the worktable surface during these procedures.Fast moving components could cause injuries.





Electrical ShockPossible danger from electrical shock. Switch off the main power and disconnect the main power connection of the instrument before replacing fuses, printed circuit boards, major sub-assemblies, etc. Replace only fuses that are externally accessible and labeled. Use only replacement fuses of the specified type and electrical rating.

Laser HazardIdentifies a Class II laser product that contains an embedded low power visible Class II laser diode barcode scanner. Warns against direct viewing into the barcode laser beam or reflections of the beam.

Hot SurfaceIdentifies an area where the operator or representative could be exposed to hot surfacese.g., heater zone 1 and heater zone 2.





Potentially InfectiousIndicates an area where the operator or representative could be exposed to potentially infectious human-sourced material. Contamination hazard from parts of the instrument that are not completely decontaminated. Appropriate personal protective equipment such as gloves, lab coat, and protective eye wear should be worn.

Chemical HazardThe operator or representative could be exposed to hazardous chemicals when handling Abbott sample preparation reagents. Exposure to hazardous chemicals is minimized by following instructions provided in product-specific labels and product-specific Material Safety Data Sheets (MSDS). Appropriate personal protective equipment such as gloves, lab coat, and protective eye wear should be worn.Do not mix any oxidizing agents such as sodium hypochlorite (household bleach) with the Lysis, Wash 1, and the Microparticle reagents in the Abbott sample preparation reagent pack. Do not mix any oxidizing agents such as sodium hypochlorite with the liquid waste of the m1000, toxic vapors can be generated from these mixtures. Pressure can build in the closed container of the mixtures. Rinse or clean the surface first with water or a weak detergent before cleaning with sodium hypochlorite.





Sharp HazardBroken glass is considered a sharp hazard.Take appropriate action when handling.Appropriate personnel protective equipment such as gloves, lab coat, and protective eye wear should be worn.




Instrument Overview

• Fully automated system to perform sample preparation.• Automates nucleic acid sample preparation through utilization of general

purpose reagents and automated liquid handling platform.• Operator controls the system via a PC, equipped with Setup and Service (S&S)

and operating (NAT) software.




Hardware Overview• Worktable, Grids, Frogs, Wash/Waste Station• X-Drives• LiHa• Lower DiTi Eject• RoMa• PosID• 1 ml Subsystem• Eight Diluters, Valves, and Syringes, VCC-Dilback Board• Door Locks (SMIO-SAFY)• FaWa, MPO Board, Pressure Relief Valve• Sensors (SMIO)• OPTIBO, Fuses, CU board, PosID CU, Servo Board• Two 1:4 Distributors • Power Supply• SMIO/SMIO-SAFY Boards




System Wiring




Worktable• A grid position defines the minimal width of carriers.• On the worktable, 45 evenly spaced guides ensure proper positioning of all carriers.

Guide pinsStop pinsGuide reference pinsCarrier lock pinsCarrier lock reference pins

• The guides enable sliding of carriers/racks in the Y-direction, needed for replacement (loading/unloading) of carriers/racks during operation and for identification of tubes, troughs, etc. on carriers by the PosID.

• Input sample carriers grids 1-6• 1 ml Subsystem grids 7-17• Wash/waste station grid 18• Reagents grids 19, 20• Carrier #1 grids 21-26• Carrier #2 grids 27-32• Reagents grids 33, 34• Output sample carriers grids 35-40







Liquid Handling Arm (LiHa)

• Has eight sampling tips arranged on one arm.• Moves left and right (X-range) driven by a Servo motor.• Each sampling tip is raised or lowered by a Servo motor within the arm.• Two additional Servo motors inside the LiHa arm drive the tips forward and

backward, and control the Y-spacing of the tips.• LiHa has independent movement in the Z-direction and has equidistant

movement in the Y-direction from 9 to 38 mm (0.31 to 1.5 in). The resolution is 0.1 mm in all three axes.

• Disposable, filtered, conductive Eppendorf 200 µL and 1,000 µL pipette tips are used.

• Integral detectors on the LiHa arm ensure tip attachment.• While using disposable tips, the electronics for the tip presence check can

control each Z-axis individually and independent of the presence or absence of a disposable tip.




LiHa




LiHa – Side

1 Support tubing guide block 2 ILID cable Freedom 3 ILID board 4 Tip adapter 5 DiTi spare set 6 Low DiTi eject option 7 Austausch ILID chip 8 Cable, ILID Freedom 9 Round cable, LiHa Freedom 10 LiHa/8 Freedom replacement 11 Support tubing




LiHa – End




Robotic Manipulator (RoMa) Arm

• RoMa arm uses grippers (eccentric fingers) to transport anything up to 400 g maximum weight.

• Resolution is 0.1 mm in all four axes (X, Y, Z, G) and 0.1 mm for rotation (R).• Rotational angle is 270.• Gripper range is 58 to 140 mm.• Precision is below ± 0.4 mm in all directions.• RoMa arm moves independently and simultaneously with respect to the LiHa.

The RoMa is mounted on the right side of the instrument, reducing the accessible work area of the LiHa on the right side.




RoMa




RoMa – Side

1 RoMa XZ Freedom replacement 2 RoMa 2 Backplane 3 DC Servo board 4 RoMa bremse (brake) 5 Riemen Y RoMa (Y-belt) 6 Eccentric finger 7 Flexcable, RoMa long 8 Flex RoMa Y-axis




RoMa – Back

1 RoMa XZ Freedom Backplane 3 Board DC Servo power 2 Device CU board 4 Flex RoMa Y-axis (flexcable)




PosID (Positive Identification)

• PosID module reads both horizontal and vertical barcodes for positive identification and positioning of carriers, racks, and containers.

• PosID uses its gripper to pull carriers toward the rear of the instrument for barcode identification on tubes, then pushes the carrier back into operating position.

• Code 2/5 Interleaved, Code 39 Standard, CODABAR, and Code 128 barcodes symbologies are read.

• Barcode density varies from 5 to 15 mil.• PosID moves in the X-direction along the worktable with a speed of

40 cm/sec.• Wavelength is 670 nm.• Laser is a Class II laser.




PosID BlockOPTIBO

Board

PosID CUBoard

DCServo

PosIDADA

Board

Y/B

DSPDecoding Board

Servo

Scanner

X Mtr

No Tube Sensor Cable

B-Motor Cable

Y-Motor Cable




PosID 2




PosID 2 Interior View

1 Z-motor fuer Genesis (PosID Y-motor) 7 Y/B board 2 DC Servo board 8 Greifer (rack gripper) 3 PosID 2 (entire assembly) 9 DSP decoding board 4 Scanner kabel (cable) 10 Tube sensor 5 Scanner motor 11 PosID module (w/o base and X-drive) 6 Laser scanner




PosID 2 – Left Service Door

1 PosID CU board 2 PosID X Drv Servo board




1 ml Subsystem – Top View

1 Heat block cover 2 Subsystem carrier 3 Subsystem carrier cover 4 Mag block and cover 5 Heatmag Subsystem (1 ml Subsystem) 6 Cable HE/MA Subsystem 7 RT block cover

1 ml Subsystem Labels




1 ml Subsystem – Bottom View

1 Heat block 2 Cable heat block 3 MBS print, complete




Diluters

1 Diluter XP3000 Plus 2 Valve, 3-way XP3000 3 Syringe, 1.0 ml




SMIO/SMIO-SAFY




SMIO/SMIO-SAFY

SMIO (Sensors) SMIO-SAFY (Locks)

J2 CAN-BUS to J3 SMIO-SAFY J2 CAN-BUS to OPTIBOJ3 CAN-BUS to J2 CAN-BUS SMIO

J9 Jumper J9 JumperJ18 System LiquidJ19 Liquid Waste J24/J5 Left Door LockJ21 Solid Waste J23/J6 Right Door LockSW1 Address 2 SW1 Address 1




Sensors




Fast Wash (FaWa) Pump Assembly

1 FaWa option (complete assay) 2 Membranpumpe KNF FaWA (Fast Wash pump) 3 Membranpumpe KNF FaWa (Fast Wash pump) 4 Check valve PP 1.55 5 MPO board 6 Verbind-Kabel 7 Konstakstreifen 12 cm (copper grounding strip)




OPTIBO Board

1 Host RS-232 2 CAN Opt 1-FaWa 3 CAN Opt 2-CU PosID 4 CAN Opt 3-1 ml Subsystem 5 CAN Opt 4-1 ml Subsystem 6 Option 6-J18 SMIO 7 LiHa J5-LiHa 8 Arm R1 J15-RoMa

6

7

8

1

2

3

4

5




OPTIBO Fuses

Fuse Assembly Amperage

1 CU Board 6.3 A6 Option 1 FaWa 87 Option 2 PosID 88 1 ml Subsystem 89 1 ml Subsystem 84 Diluters 83 RoMa 82 LiHa 85 Option 5 810 Arm L1, L2 8




Power Supply

1 Power supply




Liquid System

• The liquid system is a central component of the instrument.• Flexible tubing connects the containers, pumps, valves, and tips.• Precision diluters (1 ml syringes) ensure accurate aspiration and dispensing of liquids.• Air gaps are used to separate samples and reagents.• System liquid (water) is aspirated from the system liquid container, through aspiration tubing, via

liquid distributors to diluters.• From the diluters, liquid is channeled through the pipetting tubing to the tips. • Each tip is connected to an Integrated Liquid Detection (ILID) module.• Each tip can detect the surface of a conductive liquid by measurement of change in capacitance.• When a tip touches the liquid surface, the ILID detects the change in capacitance and triggers a

detection signal.• The wash/waste station allows for washing of the tip ends with system liquid, flushing, and for

discarding waste. • Surplus liquids are discarded into the waste container via the waste tubing.• A Fast Wash (FaWa) membrane pump for an acceleration of liquid distribution through the system

and an improved reduction of carryover.




Liquid System

30-05

30-04

9

8

30-02

3230-0730-06

8 Tips30-03

30-01

35

4

30-01 Aspiration tubing 30-07 Distributor 1:430-02 Interconnecting tubing 4 Diluter 30-03 Pipetting tubing 8 LiHa 30-04 Wash station 9 DiTi Adapter 30-05 Waste tube 32 Dilback 30-06 Y-connector 35 MPO/FaWa




Addresses




System Communication

System CAN Device Priority Jumper

Locn Terminated Address

CU (M1) D J1 No OPTIBO C SW1-1 No, switched to OFF (right) Diluters (D1-8) B J10 Yes, DILBACK board 0-7 LiHa Backplane (A1) A J14 Yes Servo 0-4

Servo Pwr 5 Option CAN Device Priority Jumper

Locn Terminated Address

CU (M1) 7 J22 No OPTIBO 6 SW1-2 No, switched to OFF (right) PosID CU (P1) 5 J6 Yes Servo 0 (X mtr) PosID CU (local CAN) PosID

J8 No Servo 2 (Y/B mtr)

MPO (01) 4 J2 No 0 SMIO-SAFY (02) 3 J9 No, jumper on inner two pins 1 SMIO (03) 2 J9 Yes, jumper on outer two

pins 2

CC Mags (04) (50°C) No 3 CC Mags (05) (75°C) No 4 RoMa Freedom BP (C1) 1 SW2-1 Yes, switched to ON (right) 4 (Device CU – X mtr) RoMa Freedom (local CAN) RoMa 2 BP

SW2-2 Yes, switched to ON (right) Servo 6 (Y/Z mtr) Servo 7 (R/G mtr)




Technical Data

• Wiring Diagram• Cable Connections• Addresses• Jumpers• CAN-BUS Resistance Test• Fuses• Motors, Servo, and Servo Power Boards• Error States




Technical Data & Requirements• Instrument

Length 142.9 cm/56.3 inLength (door opened) 176.5 cm/69.5 inDepth 76.8 cm/30.3 inDepth (front lid open) 124.5 cm/49.0 inHeight (RoMa extended) 127.0 cm/50.0 inWeight 178.3 kg/393.0 lbClearance 1 m (39 in) around the instrument

4 in minimum rear clearance• Cabinet

Length 142.9 cm/56.3 inDepth 79.4 cm/31.3 inHeight 76.2 cm/30.0 inWeight 103.4 kg/228.0 lb




Electrical Requirements

• Line Voltage• Frequency• Power• Equipment and Safety Class• Circuit Breaker• Electrical Outlets Required• UPS

100 to 240 VAC ± 10%50 to 60 Hz1200 VA115 AMinimum of fourON3600Abbott Labs 3600 VA/2520 Watts/16 Amp LN 7G52-20




Delivery Requirements

• Loading dock• Load capacity of elevator• Minimum size of elevator

• Door and entryway openings• Passageway corners

• Personnel

• Tool requirements

PresentMinimum 400.0 kg (882 lb)Refer to cabinet and instrument dimensionsLarger than 81.5 cm (32.0 in)Combined total width of both passageways around corners must be greater than 279.4 cm (110.0 in)Four people are required to lift the m1000Lifting device (four handles, two pipes, and four safety pins)




Cabinet Installation

• Unpacking and Positioning • Cabinet Installation• Cabinet Leveling

(Reference Service Manual, Chapter 4)




Instrument Installation

• Lifting instrument• Instrument setup• OPTIBO board• Syringe, valves, tubing, and 1:4 distributors• FaWa tubing routing• 1 ml Subsystem cables (bottom two on OPTIBO right side, grids #10 and 15)• Sensor cables (three)• System liquid and waste containers• Unpack and install the computer stand, PC, monitor, printer, keyboard, and mouse• Connect cables to the rear of the PC• Connect the RS-232 cable between the PC and instrument• Connect the power cords• Switch on power




Genesis (S&S) Software Installation

• Run Setup.exe in D:\Disk1 directory• Choose destination location• Select components• Select program folder• Password• Main window• Move pages




CD Menu




Disk 1




Welcome Install Shield




Software Release Notes




Software Destination Location




Software Select Components




Select Components Menu View 1




Select Components Menu View 2




Software – Select Program Folder




Software – Start Copying Files




Install Shield Wizard Complete




S&S – Programs Menu




S&S – Main ScreenMenu Bar

Tool Bar

Worktable

Status Bar




S&S – Menu Tool Bar




S&S – Expanded Menus




S&S – System Menu

• Passwordsmart

• COM port• Initialize Instrument• Load Worktable

NAT MBS Worktable• Test Configuration• Print Setup• Print• About

Software version




NAT Worktable




S&S – View Menu

• WorktableCarriers, Racks, Zoom Active

• Tool Bar• Status Bar




S&S – Instrument Menu




S&S – Instrument Menu/Basic Setup

• Basic SetupSerial Number SettingsFirmware DownloadEEPROM Backup/RestoreWorktableSet X Drive PropertiesSet Machine SizeInformationPrintingTest Report




Basic SetupSerial Number Settings




Basic SetupSerial Number Settings Tab




Basic SetupFirmware Download Checked




Basic SetupFirmware Download Tab




Basic SetupEEPROM Backup/Restore




Basic SetupEEPROM Tab




Basic SetupWorktable Tab




Basic SetupInformation Tab




S&S – Instrument Menu/Command Tool

• Command ToolBatch File ExecutionSingle Commands




Command ToolSingle Line Commands Example




S&S – Instrument Menu/Information

• InformationResultsInformationVersion Printing




Information PanelResults Tab




Information PanelInformation Tab

Tree view of the device ormodules from which info

can be retrieved

Displays info about selected item in the upper list




Information Panel Version Tab




System Devices Menu




S&S – System Devices/PosID

• Mechanical Adjustment• Setup• Barcode Types• Test• Movement




PosID Setup Menu




PosID Barcode Settings




PosID Test Configuration




PosID Test – [MORE]Advanced Configuration




S&S – System Devices/LiHa

• LiHaSet Default ParametersDetermine RangeCalibrate Lower DiTi EjectDetermine Reference PositionsDetermine SpacingIndividual-ZArm Position AccuracyTip AdapterInformationPrintingTest Report




LiHa Contents Tab




S&S – System Devices/LiHa/Channel Page

• Channel PageTip ConfigurationLength of user defined tipsSyringe sizeLow Volume Option




LiHa Panel Channel Tab




S&S – System Devices/LiHa/Setup Page

• Setup Page—provides controls to set parametersfor various setup procedures

Determine Reference Positions– automatically– manually

Reference Positions– Use for Y– Grid

4/8 Reference Tips AvailableUse Y Backlash




LiHa Panel Setup Tab




S&S – System Devices/LiHa/Diluter Tool

• Diluter Tool—for checking diluters and associated tubing

Aspirating/dispensingFlushing diluters sequentiallyFilling/emptying system




LiHa Panel Diluter Tab




S&S – System Devices/LiHa/Information

• Information—summarizes device parameters and diagnostics data

Device Parameters– Axes (movement axis X, Y, Ys, Z1-Z8)– Offset (for each axis in mm)– Displacement (for each axis)– Range (for each axis)– Scale (factor)– Acceleration (for each axis [mm/sec/sec])– Speed (high speed [mm/sec])– Move Speed (working speed)




S&S – System Devices/LiHa/Information (Continued)

• InformationDiagnostics Data

– Axes– Moves (# of overall moves/axis)– Moves (# of clearable moves/axis)– Distance (total moved distance [in meters/axis])– No-Loads (crashes)– Fetched DiTis– Piercings– Status (ready, initialization errors, not implemented, not initialized,

not loaded, unknown)




LiHa Panel – Information Tab




LiHa Panel – Move LiHa Tab




LiHa Panel – Tip Adapter TestTest Configuration Tab




LiHa Panel – Individual-Z AlignAlignment Tab




S&S – System Devices/Move Test

• Move Test—verifies correct functioning of the LiHa, RoMa, PosID, and Diluters drives

Range TestRandom Move Test—equivalent to Burn-In




Move Test – Test Results Tab




Move Test – Random Test Configuration Tab




S&S – System Devices/RoMa/Pages (Tabs)

• Contents• Setup• Test Configuration• Printing• Move RoMa• Information




S&S – System Devices/RoMa/Contents Page

• Available Devices• Setup

Set Default Parameters (factory set!)Determine RangeSet TypeDetermine Reference PositionsAdjust Rotator

• TestReference Plate




RoMa – Contents Tab




S&S – System Devices/RoMa/Setup Page

• Setup page—provides controls to set parameters for various setup procedures

Rotator TypeGripper TypeZ-rangeReference Positions

– Grid– Use for Y

Ref. Plate Pos. GridInitialize Position X




RoMa – Setup Tab




S&S – System Devices/RoMa/Test Configuration Page

• Test Configuration—page displays when ‘Reference Plate Test’ (Contents page) is selected

Ref. Plate Pos. GridCycles




RoMa – Information Tab




Move RoMa Tab




Liquid System – Menu

• DiTi• Liquid Detection• Gravimetric Test—not used• FaWa• 6-way valve—not used• Licos (MPO)—not used• Licos (Supervisor)• Balance—not used




Liquid System – DiTi

• DiTi Eject TestNot used on the m1000

• Lower DiTi Eject TestTests the putback and disposal ejection heights for the lower DiTi eject. The calibration function must be performed before the lower DiTi eject test is used.




DiTiLower DiTi Eject Test




Liquid System – DiTi/Lower DiTi Eject Test

• Configure the Lower DiTi Eject TestDiTi Type: 1000F EDiTiSource Rack: DiTi.RRFirst DiTi Position: 1Destination Rack: DiTi.RFPut back DiTi on lower position: leave blankWaste Rack: W1.DiTiWasteOperator Name:[Select Tips]




Lower DiTi Eject Test Screen




Liquid System – DiTi/Liquid Detection

• Setup• Normal Test• Extended Test




Liquid Detection – Setup




Liquid System – DiTi/Liquid Detection/Normal Test

• Cycles: 15• Submerge: 10• Is the Liquid Source a Rack?: • Source Rack: T1.RF• Flush Rack: W1.Waste,RM• Destination Rack: C4.R1• First Dest. Position: 1• DiTi:

DiTi Type: 1000F EDiTiDiTi Rack: DiTi.RRDiTi Waste Rack: W1.DiTiWasteFirst DiTi Position: 1




Liquid Detection Test[Select Tips]




Liquid Detector Test –Advanced Configuration




Liquid System – DiTi/Liquid Detection/Normal Test/Fill/Flush

• Select [Fill/Flush]. ‘Fill Liquid System’ screen displaysUse Fast Wash Pump: checkedFlush Station: W1.WASTE,RMWash Station: not highlightedDiTi Waste Rack: W1.DiTiWasteSystem Liquid Aspirate Speed: 1000 µL/sFill Speed: 1000 µL/sFill Liquid Volume: 30000 µL

• Select [Fill System]




Liquid System – FaWa

• Setup• Test




Fast Wash Pump Setup




Liquid System/FaWa Pump Test Configuration

• Prefill Volume: 600 µL• Destination Rack: C3.R1, Grid 4• Flush Rack: W1.Waste,RM• DiTi Type: 1000F EDiTi• DiTi Rack: DiTi.RR, Grid 21• DiTi Waste Rack: W1.DiTiWaste• First DiTi Position: 1




Liquid System/FaWa Pump Test Configuration/[More]

• Select [More]. ‘Advanced Configuration’ screen displaysUser Defined: checkedName of: name the testDuration: 30 (typically)Minimal Throughput: 1600 µL/s (typically)Test settings: current test settings[Selected Tip Type]: current selected tipNumber of used Tips:Duration:Minimal Throughput to pass Test:




Liquid System/FaWa Pump Test Configuration/Fill/Flush

• Select ‘Liquid System’/‘FaWa’/‘Test’• Select 1000F EDiTi as the DiTi Type• Select [Fill/Flush]. The ‘Fill Liquid System’ screen displays

Use Fast Wash Pump: selectedFlush Station: W1.WASTE,RMDiTi Waste Rack: W1.DiTiWasteSystem Liquid Aspirate Speed: 1000 µL/sFill Speed: 1000 µL/sFill Liquid Volume: 30000 µL

• Select [Fill System] to initiate filling of the dispense system




FaWa Pump[Fill/Flush]




Options Menu

• Easy Option—not used on the m1000• Heated Incubator—not used on the m1000• Plate Washer—not used on the m1000• Access Status Options• Incubation Device• SMIO for NAT




Options/Access Status Options

• Contents PageSelect ‘Access Status’ from ‘Available Devices’Select ‘Device Test’ under ‘Test’

• Configuration & ToolsInformal testing

– Select ‘Door Lock 1 (left)’ and ‘Door Lock 2 (right)’– Select [Lock] then [Unlock] to check the door lock function

Formal testing– Select [Start]– Follow prompts to test the left and right door locks




Access Status Contents Tab




Access/StatusConfiguration & Tools Tab




Options/Incubation Device• Available Devices• Setup

Install Plates/Create or Edit Custom Plates• Test

Burn-In of HeatingHeating Validation with TEMPO110 (optional)




Options/Incubation Device/“Install Plates/Create or Edit Custom

Plates”• “Plate Installation” tab displays

Select NAT 50/75°C plate from “Available Plates”Select “TEMPO110” option under “Heating Block Calibration by” menu[APPLY][START]




Plate Installation Tab




Options/Incubation Device/Burn-In of Heating

• Used when a new heating block is installedSelect “ Burn-In of Heating”Select the “Test Configuration” tab[START]




Test Configuration




Options/Incubation Device/ Heating Validation with TEMPO110

(Optional)• Used to verify heater block performance using the

TEMPO110 measuring deviceSelect ‘Heating Validation with TEMPO110’ under “Test”Switch to “Test Configuration” tab

– Choose either 50 or 75°C (50°C is the default, double click on 50 to edit to 75°C)

– Input name in the ‘Operator Alias’ field– Select [APPLY], [START]– Pass: 50 ± 1.5 75 ± 2.3




Options/SMIO for NAT

• Setup: DiTi Waste Sensor CalibrationCalibration of DiTi Waste Sensor

• Test: SensorsSystem Liquid and Liquid Waste Sensor Test




Options/SMIO for NAT/DiTi Waste Sensor Calibration

• Contents page: Select ‘DiTi Waste Sensor Calibration’• Select ‘Tools & Configuration’ tab

Verify Device Type is NATSelect only ‘DiTi Waste Full’ under ConfigurationFollow onscreen prompts




SMIO Panel Contents Tab




SMIO PanelTools & Configuration Tab




Options/SMIO for NAT/Rapid Sensor Test

• Select the ‘Tools & Configuration’ tab• Check the box(es) of sensor(s) to be tested

System Bottle Empty– Remove the system bottle. The panel displays ‘yes’ and the box is shaded.– Place your hand in front of the sensor. Panel changes to ‘no’.

Wash Bottle Full– Remove the wash bottle then place your hand in front of the sensor.– Panel changes from ‘no’ to ‘yes’ and the box is shaded.

DiTi Waste Full– Place your hand just below the sensor. Panel changes from ‘no’ to ‘yes’ and

the box is shaded.




Options/SMIO for NAT/Accurate Sensor Verification

• System BottleSelect the ‘Sensors’ Test box on the Contents pageSelect the ‘Tools & Configuration’ tab then check the ‘System Bottle Empty’ boxSelect [START]Follow onscreen prompts

• Liquid Waste BottleSelect the ‘Sensors’ Test box on the Contents pageSelect the ‘Tools & Configuration’ tab then check the ‘Wash Bottle Full’ boxSelect [START]Follow onscreen prompts

• DiTi Waste Sensor VerificationSelect the ‘Sensors’ Test box on the Contents pageSelect the ‘Tools & Configuration’ tab then check the ‘DiTi Waste Full’ boxSelect [START]Follow onscreen prompts




Labeling

• Follow instructions for labelingWorktable surface1 ml SubsystemInput and Output carriers and racks

– Depends on ‘closed’ or ‘open’ mode

Reagent trough carriers and troughs




NAT Software Installation

• Insert the Installation CD into the CD-ROM drive.• Select the CD-ROM drive from ‘My Computer’.• Initiate NAT software installation and follow the prompts.• Select ‘Abbott Magnetic Bead Separation’ worktable.• Continue following the prompts.• When prompted to reboot the computer, select [NO]. • Installation takes approximately 15 minutes.• Select [OK] when prompted to create an administrator user and

password.• Create a User Name and temporary password. Select [OK].• Select [Finish>]. Reboot the computer.




Register NAT Software• Start ‘NAT Wizard’ software.• The ‘NAT Software Evaluation License’ dialog window displays.• Select ‘Register your installation now’ then select [OK].• Select ‘Order NAT Software to obtain a valid license.’• Select ‘No’ if the NAT software serial number is available on the CD package.• Select ‘Yes’ if the NAT software needs to be ordered.• Complete the ‘NAT MBS’ form.• Type the serial number in the field ‘Used Tecan Instruments:’.• Select [Next>], [I agree], then [Next>] to continue.• Send the license agreement to Tecan.• After the License ID is received from Tecan, restart ‘NAT Wizard’.• Enter the SN next to the ‘Enter the NAT MBS serial number’ option.• Enter the License ID next to the ‘Enter the NAT MBS license number’ option.• When the License ID is registered, a confirmation screen displays.• Select [Finish].




Calibrate 1 ml Subsystem for LiHa• Mount the reference tip to DiTi #3.• The following positions from the ‘Current Rack’ field are calibrated one location at a

time:1 ml Heater Zone 1 Calibration Point1 ml Heater Zone 2 Calibration Point1 ml Mag Calibration Point1 ml RT Calibration Point1 ml Mag Rest Calibration Point (align flush with surface, not in the groove)

• Select ‘Travel Home’ to home the LiHa.• Select ‘Travel with the LiHa to Location on Carrier’.• Use the movement keys to align the reference tip with the centered indentation located on

each of the locations. Touch the surface.• When aligned, select ‘Save LiHa Calibration’.• Verify alignment by selecting ‘Travel Home’ then ‘Travel with the LiHa to Location on

Carrier’.• Repeat alignment, if necessary.• Calibrate the next position.• Remove the reference tip after all positions are calibrated.




Calibrate 1 ml Subsystem for RoMa

• The following positions from the ‘Current Location’ field are calibrated one location at a time (carrier with cover in place at each location):

1 ml Subsystem Loc Heater Zone 11 ml Subsystem Loc Heater Zone 21 ml Subsystem Loc Mag Low1 ml Subsystem Loc RT1 ml Subsystem Loc Rest

• Select ‘Travel with the RoMa to the Location’.• Move the gripper arms so they are just below the lip on the sides of the carrier

and centered on both sides of the carrier. The gripper pins on the RoMa should fit in the indentations on the side of the carrier.

• When aligned, select ‘Save RoMa Calibration’.• Verify alignment by selecting ‘Travel Home’ then ‘Travel with the RoMa to

the Location’. If not aligned, repeat the procedure.




Calibrate DiTi Rack Carriers for RoMa

• Select a carrier from the ‘Current Location’ field:Plate Carrier 2, Loc 1Plate Carrier 2, Loc 2Plate Carrier 2, Loc 3

• Position the DiTi rack being calibrated then select ‘Travel with the RoMa to the Location’.

• The RoMa gripper arms should be centered on both sides of the DiTi rack and approximately 1 mm (10 steps) above the surface of the DiTi rack carrier.

• Touch the DiTi rack with the gripper pins then move the gripper arm 1 mm (10 steps) to the right in the X-direction.

• When aligned, select ‘Save RoMa Calibration’.• Verify alignment. Select ‘Travel Home’ then ‘Travel with the RoMa to the

Location’. Repeat alignment if necessary.• Calibrate other positions.




Calibrate DiTi Rack Shelves for RoMa

• Select a location from the ‘Current Location’ field:Select Shelf Loc 1 through Shelf Loc 8

• Select ‘Travel with the RoMa to the Location’.• The RoMa gripper arms should be just touching on the surface of the DiTi

rack shelf and centered on both sides of the rack.• When the RoMa gripper pins are touching the rack, back off the gripper arms

1 mm (10 steps). For Shelf Loc 1 only—move the arms 2 mm (20 steps) to the left.

• Select ‘Save RoMa Calibration’.• Verify alignment. Select ‘Travel Home’ then ‘Travel with the RoMa to the

Location’. If alignment is acceptable, select ‘Travel Home’.• Calibrate other shelf locations.




Check DiTi Locations

• From the Main Menu, select ‘Select Protocol’.• Select ‘Check DiTi Locations’ protocol and ‘>’.• At the sample screen, add a sample rack with 8 barcode labeled tubes at grid 4,

5, or 6.• Select ‘Scan Rack’ and ‘>’ to continue.• Select ‘>’ to continue.• Select ‘Using Full DiTi Racks’ at the Load DiTis prompt.• Add full 1,000 µL DiTi Racks to Plate Carrier 1, Loc 1, 2, and 3 and Plate

Carrier 2, Loc 1 and 2.• Add a full 200 µL DiTi rack to Plate Carrier 2, Loc 3.• Proceed with the prompts.• Select ‘Use Full DiTi Boxes only’.• Determine if the DiTi cones are centered over the DiTis as they are picked up.

Verify all six locations.




Calibrate DiTi Rack Carriers for LiHa

• Remove the DiTi rack carriers.• Select ‘Calibrate LiHa’.• Select any or all of the Plate Carrier locations from the

‘Current Rack’ field if alignment was off when the Check DiTi Locations protocol was performed.

• Adjust only in the X- and Y- directions, except Plate Carrier 1, Loc 3 (DiTi Reuse Rack). For only this carrier, raise the Z-direction carrier 28 steps.

• After each location is adjusted, select ‘Save LiHa Calibration’.




Move Plate Test

• From the Main Menu, select ‘Select Protocol’.• Select ‘Move Plate Test’ then follow the prompts.• At the ‘Sample’ screen, add a sample rack with eight barcode labeled tubes to

grid # 1, 2, 3, 4, 5, or 6.• Follow the prompts then select ‘Using Full DiTi Racks’. Follow the prompts.• The RoMa moves the 1 ml Subsystem carrier to different locations in the

1 ml Subsystem.• When prompted, place a full 1,000 µL DiTi rack in Plate Carrier 2, Loc 1 and

a full 200 µL DiTi rack in Plate Carrier 2, Loc 3.• Ensure all other carrier and shelf locations are empty. Follow the prompts.• The RoMa moves racks back and forth from carrier locations to shelf

locations.• If necessary, repeat the NAT Teach Worktable racks and locations.




Troubleshooting• Sensor Failures• Door Lock Failure• 1 ml Subsystem Heater Failure• Fast Wash Pump Failure• PC Not Communicating with m1000 Instrument• Power Supply• RoMa (rotator, gripper, Y-, Z-, X- Direction)• LiHa (X-, Y-, Z- Direction, DiTi Ejection)• PosID (X-, Y-, Tube Sensing, Barcode Reading)• Level Sense Failures• Precision Test Failed• FaWa Pump Test Failed




TroubleshootingSingle Line Commands

• A1SAX9999A = Device1 = AddressSAX = Command (typically 3 letters)9999 = Parameters/argument





• A1 = LiHa• P1 = PosID• M1 = Machine• D1-8 = Diluters• C1= RoMa





• CommandsS = Set M1SMA (set machine all Axes)P = Position C1P1Z (initialize Z-Axis)R= Report M1RFV (report firmware)





• M1PIS – Position Init SystemInitializes the LiHa, RoMa, and PosIDResponse: @’, 1, or 5Errors:

– (1) initialization failed– (5) device not implemented





• M1REE – Report Extended Error Code LiHa, PosID, and RoMa

Response: A@, P@, and R@Errors: none





LiHa A1 Initialization CommandsA1PIA

Position Initialization X/Y/Z- AxesResponse: @’, 1, or 5Errors:

– (1) initialization failed– (5) device not implemented





LiHa A1 (Position Commands)

• Device: A1• Command: PAA (X, Y, Ys, Z1, Z2, Z3, Z4, Z5, Z6, Z7, Z8)• Explanation: Position Absolute for All Axes• Example: #A1PAA1000,100,500,500,500• Explanation: LiHa moves in X, Ys, Z1, Z2, and Z3 to specified coordinates• Errors:

(3) invalid operand(7) device not init(10) drive no load(13) arm collision avoided with PosID(17) arm collision avoided with RoMa






• Device: A1• Command: PAX• Explanation: Position Absolute for X-Axis• Example: • Explanation: • Errors:

(3) invalid operand(7) device not init(10) drive no load(13) arm collision avoided with PosID(17) arm collision avoided with RoMa






• Device: A1• Command: PAY Y*, Ys*• Explanation: Position Absolute for Y-Axis• Example: A1PAY500,200• Explanation: Absolute move in Y-direction to 50 mm and a tip space of

20 mm• Response/Errors:

@(3) invalid operand(7) device not init(10) drive no load(13) arm collision avoided with PosID






• Device: A1• Command: PAZZ1*, Z2*, Z3*, Z4*, Z5*, Z6*, Z7*, Z8*• Explanation: Position Absolute for Z-Axis• Example: • Explanation: • Response/Errors:

@(3) invalid operand(7) device not init(10) drive no load





RoMa C1 (Initialization Commands)

• Device: C1• Command: PIA• Default Speed:• Range:• Explanation: Position Initialization X/Y/X/R/G- Axes:• Response: @, 1, or 5• Errors:

(1) initialization failed(5) device not implemented






• Device: C1• Command: PIX• Default Speed: 400• Range: 5 to 1000• Explanation: Position Initialization X-Axis:• Response: @, 1, 3, or 5• Errors:

(1) initialization failed(3) invalid operand(5) device not implemented






• Device: C1• Command: PIY• Default Speed: 350• Range: 5 to 1500• Explanation: Position Initialization Y-Axis:• Response: @, 1, 3, or 5• Errors:







• Device: C1• Command: PIR• Default Speed: 500• Range: 5 to 1500• Explanation: Position Initialization R-Axis (Rotator):• Response: @, 1, 3, or 5• Errors:







• Device: C1• Command: PIG• Default Speed: 300• Range: 5 to 1500• Explanation: Position Initialization G-Axis (Gripper):• Response: @, 1, 3, or 5• Errors:







• Device: C1• Command: PAX, PAY, PAZ, PAR, or PAG• Explanation: Position Absolute for X-, Y-, Z-, R-, or G- Axis• Response: @’, 1, 3, 5, 7, 10, 17• Errors:

(1) initialization failed(3) invalid operand(5) device not implemented(7) device not initialized(10) drive no load(17) arm collision avoided with LiHa





• Device: C1• Command: RPX, RPY, RPR, RPZ, or RPG• Argument:

0 current position in 1/10 mm1 acceleration in 1/10 mm/J2

2 end speed in 1/10 mm/s3 init speed in 1/10 mm/s4 offset in 1/10 mm5 machine range in 1/10 mm

• Explanation: Report Current Parameter for X-, Y-, R-, Z-, or G- Axis• Response: value or 3• Errors:

(3) invalid operand




Preventative Maintenance

• Perform every six monthsReplace DiTi cones, tubing extensions, ringsClean partsPerform tests

• Perform every 12 monthsPerform six month PMReplace: ILID cables; syringes; valves; aspiration, pipetting, waste, and FaWa tubing; clamps; Y-distributor for tubing; liquid waste assembly; system liquid assembly




Spare Parts

• Service Manual – Chapter 11• FRUD




R&R

• Worktable




R&R – System Electronics

• OPTIBO• FUSES• CU Board• SMIO/SMIO-SAFY Boards• Power Supply




R&R – X-Drive Assemblyfor LiHa and RoMa

• X-Drive Assembly• X-Slide• X-Drive Belt




R&R – LiHa• Complete LiHa• Backplane• DC-Servo Board• Servo Power Board• ILID Board• ILID Chip• ILID Board Flat Cable• ILID Cable• Y-Belt• Y-Motor• DiTi Adapter (DiTi Spare)• Lower DiTi Eject • Solenoid• Tip Adapter




R&R – RoMa

• Complete RoMa• Grippers (eccentric fingers)• Gripper Board• Rotator Motor• Y-Flex Cable• Gripper/Rotator Flex Cables• Y/R- and Z/G- DC-Servo Motor Boards• RoMa 2 Backplane• Y-Belt• Y-Motor• Z-Motor• Z-Brake• RoMa Freedom Backplane• Device CU• X-DC-Servo Power Board




R&R – PosID

• Complete PosID Assembly• X-Drive Assembly (belt, motor, flex cable)• PosID CU Board• X-DC-Servo Motor Board• Y/B Board• Y/B-DC-Servo Motor Board• DSP Decoding Board• Scanner Assembly

B-MotorScanner HeadScanner Cable

• Y-Drive Assembly (belt, gripper assembly, motor)• No Tube Sensor




R&R – Tubing System

• Aspiration Tubing• Interconnecting Tubing• Pipetting Tubing• Wash/Waste Station• Waste Tubing• Y-connector• 1:4 Distributor




R&R – Monitored Pump Option/Fast Wash Option

• MPO/FWO Complete Assembly• Fast Wash Pump• Pressure Relief Valve• MPO/FWO Tubing• MPO Board




R&R – Diluters

• Diluter XP 3000 Plus• 3-way Valve• Syringe• VCC-Dilback




R&R – Sensors and Locks

• Level SensorsInstallationCalibration of solid waste sensorAdjustment of liquid level sensor potentiometersVerification

• Door LocksInstallationVerification




R&R – 1 ml Subsystem

• Installation• Verify heater zones 1 and 2• 1 ml Subsystem heater block• 1 ml Subsystem heater boards• 1 ml Subsystem internal cables• 1 ml Subsystem cables, from OPTIBO board




COM Port SettingExample

• Network Dial-up• Advanced• Network Identification• System Properties• Hardware• Device Manager• Communication Port• Port Settings• Advanced




CAN-BUS Resistance Test

• SYSTEM CANDILBACKLiHa BackplaneCU BoardOPTIBO

• OPTION CANCU BoardPosID CU BoardOPTIBOCC MAGSCC MAGSMPORoMa BackplaneSMIOSMIO-SAFY




CAN-BUS Resistance Test

CAN-BUS Check Points Resistance InterpretationSystem CAN-BUS CAN_L/CAN_H 50 to 60 Ohms Correct # of jumpers

~ 40 1 jumper too many~ 120 1 jumper too few

Option CAN-BUS CANO_L/CANO_H 50 to 60 Ohms Correct # of jumpers~ 40 1 jumper too many~ 120 1 jumper too few




System and Option CAN-BUSJumper Settings

Jumper LocationCAN-BUS Present

SYSTEM CANDILBACK Yes J10 (not labeled)LiHa Backplane Yes J14CU Board No J1OPTIBO No SW1 Off

OPTION CANCU Board No J22PosID 2 Yes J6

No J8OPTIBO No SW1-2 OffCC MAGS #3 No no jumperCC MAGS #4 No no jumperMPO No J2RoMa Backplane Yes SW2 OnSMIO Yes J9 (outer two pins)*SMIO-SAFY No J9 (inner two pins)** Jumper location must be on the inner two pins on one board, and on outer two pins on the other board

m1000™ Service Training Guide Supplement-169528-101—September 2003

m1000 Instrument Service Training—Review and Test Questions

Name: _________________________________________

Date: ___________________________________________


1. What boards and motors are shared by the RoMa, LiHa, and PosID?

2. How does the m1000 detect which board controls a particular function?

3. How many different types of grids are on the worktable?

4. Where are the reference grids located?

5. Which board monitors the door locks?

6. Which board monitors the system liquid, liquid waste, and solid waste sensors?

7. List the two amperage ratings of fuses used on the m1000.

8. Where is the CU board located?

Supplement-2 m1000™ Service Training Guide69528-101—September 2003

9. What size fuse is used for the PosID?

10. To what address should the address switch (SW1) on the SMIO-SAFY board be set?

11. The CAN-BUS cable that connects the SMIO to the SMIO-SAFY board goes from J__ on the SMIO board to J__ on the SMIO-SAFY board.

12. Can the jumpers on J9 on the SMIO and SMIO-SAFY boards be set the same?

13. What is a quick indicator that the instrument is functioning properly?

14. How many motors are used in the LiHa?

15. What is the principal visible component difference between the SERVO and SERVO POWER boards?

16. When a RoMa is replaced, what are some critical considerations to review?

17. Which barcodes are read by the PosID?

18. List the primary components of liquid level sensing.



19. The m1000 performs which of the following?

a) Sample/specimen preparationb) Nucleic acid amplificationc) Automated amplified nucleic acid detection

20. What size syringes does the m1000 use?

a) 250 µLb) 500 µLc) 1,000 µLd) 1 mle) 5 ml

21. The PosID uses a _______________ _______________ to pull carriers past the barcode reader.

22. The PosID uses a _____________ _________________ to detect the presence or absence of tubes.

23. Two voltages used in the m1000 are _________ Volts and ________ Volts.

24. List the address settings for the following boards:

LiHa DC Servo board (position 2)LiHa DC Servo Power boardRoMa Freedom Backplane Device CU boardRoMa Freedom Backplane Power boardFaWa MPO board

25. What assemblies are located on the system CAN-BUS line?


26. What assemblies are located on the Option BUS line?

27. What do fast blinking red and green lights on the CU board indicate?

28. To what connection on the OPTIBO board should the cable from the 50°C heating block of the 1 ml Subsystem be plugged in?

29. When the S&S software is being installed, what options should be selected in the Select Components screen?

30. What is the field service password for the S&S software?

31. Which worktable is used for the m1000?

32. Which two buttons in the S&S software are used the same way to initialize the instrument?

33. During the Tip Adapter Test for the LiHa, the FSR observes the progress bar which indicates the tip was detected but the test fails. Why?

34. Why is the LiHa right reference position 40 and not 45?

35. Why is the RoMa left reference position 7, not 1?



36. What rotator type, gripper type, and Z-range are used on the m1000 RoMa?

37. When testing the system CAN-BUS line to determine if the correct number of jumpers are installed, the resistance should be approximately _________ Ohms.

38. How is the functionality of diluter #6 verified?

39. How is the connection of diluter #2 to LiHa axis #2 confirmed?

40. There are three ways to fill/flush the liquid system. List two of the three ways.

1)2)

41. How should a backup of the EEPROM parameters of the LiHa be performed?

42. What should be checked if a liquid bottle sensor is failing? If multiple sensors are failing?

If a single sensor is failing, check:••••

If multiple sensors are failing, check:•••


43. One heater on the 1 ml subsystem has failed. What should be checked?

The following should be checked:•••••

44. On the pressure relief valve, to what level should the pressure be adjusted?

45. An FSR replaced a failed diluter and the diluter still does not work. What is a possible reason for the failure?

46. An FSR replaced a RoMa and now it rotates CCW instead of CW. What could be wrong?

47. Two LiHa axes, Z7 and Z2, are not functioning. Which Servo board controls these two boards?

48. List the order of insertion of the black O-ring and white separator ring, when the two rings on the DiTi Adapter are replaced.

49. To what connector on the Tip Adapter board should the ILID cable be connected?

50. What functions do the RoMa Freedom Backplane and the RoMa 2 Backplane control?

51. Where is the EEPROM for the RoMa located?



52. Outline the flow/route of liquid from the system liquid bottle to the DiTis.

� � � � � � � � � � � � � � � � .

53. To what connector(s) on the SMIO-SAFY board is the left door lock connected?

54. What is the protocol to verify that heater zone 1 is heating to 50°C ± 2.5°C?

55. How is firmware downloaded?

56. If the machine size of the m1000 is not set to 150, what should be done?

57. If the instrument type is not Freedom, what should be done?

58. What is the change position of the PosID?

59. What test is performed to determine if tips are picked up and disposed of correctly?


60. What test is performed to verify that liquid is sensed and detected consistently and accurately?

61. An FSR replaced a FaWa pump assembly and the pump does not work. What is the problem?


m1000 Instrument Service Training—Review and Test Questions with Answers

Name: _________________________________________

Date: ___________________________________________


1. What boards and motors are shared by the RoMa, LiHa, and PosID?

Servo PCB (619303) and Motor (619323). (Ref. p3-24)

2. How does the m1000 detect which board controls a particular function?

The m1000 uses the CAN-BUS network together with the addresses on the boards to identify its function.

3. How many different types of grids are on the worktable?

There are five different types of grids on the worktable:• guide• guide reference• carrier lock• stop pins• carrier lock reference

4. Where are the reference grids located?

Reference grids are located at grids 1, 7, 12, 35, 40, and 45.

5. Which board monitors the door locks?

Door locks are monitored by the SMIO-SAFY board. (Ref. Chapter 9)

6. Which board monitors the system liquid, liquid waste, and solid waste sensors?

System liquid, liquid waste, and solid waste sensors are monitored by the SMIO board. (Ref. Chapter 9)

7. List the two amperage ratings of fuses used on the m1000.

Fuses are either 6.3 or 8 Amps. (Ref. Chapters 3 and 9)

8. Where is the CU board located?

The CU board is attached to the solder side of the OPTIBO board. (Ref. Chapter 9)


9. What size fuse is used for the PosID?

The PosID fuse is 8 Amps. (Ref. Chapter 3)

10. To what address should the address switch (SW1) on the SMIO-SAFY board be set?

The address should be set to 1 for the SMIO-SAFY board (2 for the SMIO). (Ref. Chapter 9)

11. The CAN-BUS cable that connects the SMIO to the SMIO-SAFY board goes from J__ on the SMIO board to J__ on the SMIO-SAFY board.

The CAN-BUS cable goes from J2 on the SMIO board to J3 on the SMIO-SAFY board. (Ref. Chapters 3 and 9)

12. Can the jumpers on J9 on the SMIO and SMIO-SAFY boards be set the same?

No.

13. What is a quick indicator that the instrument is functioning properly?

A blinking green light indicates that everything on the CU board is running as expected. (A board or motor failure is not indicated by the LED.) (Ref. Chapter 3)

14. How many motors are used in the LiHa?

Eleven motors (8 Z, 2 Y, and 1 X) are used. There are three different types of motors used. (Ref. Chapter 3)

15. What is the principal visible component difference between the SERVO and SERVO POWER boards?

The SERVO board has a green resistor. The SERVO POWER board has a brown resistor. (Ref. Chapter 9)

16. When a RoMa is replaced, what are some critical considerations to review?

When a RoMa is replaced, verify it is set up properly in the software [Rotator Type: CW, Gripper Type: Eccentric, Z-range: Extended (long)]. In addition, the RoMa must be aligned with the guide pins on the worktable. (Ref. Chapters 9.7.4 and 13.15.4)

17. Which barcodes are read by the PosID?

The PosID barcode reader can read up to 22 different barcodes stored as an .ini file in the Setup & Service software, which needs to be updated and sent before the test. The NAT software is configured to read Code 2/5 Interleaved, Code 39 Standard, CODABAR, and Code 128). (Ref. Chapters 4.4.8 and 13.12.4)

18. List the primary components of liquid level sensing.

LiHa, ILID board, ILID chip, DiTi Adapter, Tip Adapter, ILID cable, Worktable DiTis. (Ref. Chapter 9.9.2)



19. The m1000 performs which of the following?

a) Sample/specimen preparationb) Nucleic acid amplificationc) Automated amplified nucleic acid detection

X a) Sample/specimen preparation.

20. What size syringes does the m1000 use?

a) 250 µLb) 500 µLc) 1,000 µLd) 1 mle) 5 ml

X c) and d) (1,000 µL and 1 ml are the same)

21. The PosID uses a _______________ _______________ to pull carriers past the barcode reader.

The PosID uses a carrier gripper to pull carriers past the barcode reader. (Ref. Chapter 13.12.2)

22. The PosID uses a _____________ _________________ to detect the presence or absence of tubes.

The PosID uses a tube sensor to detect the presence or absence of tubes. (Ref. Chapter 13.12.3)

23. Two voltages used in the m1000 are _________ Volts and ________ Volts.

Two voltages used in the m1000 are 24 Volts and 5 Volts. (Ref. Chapter 3)

24. List the address settings for the following boards:

LiHa DC Servo board (position 2) 3LiHa DC Servo Power board 5RoMa Freedom Backplane Device CU board 4RoMa Freedom Backplane Power board 0FaWa MPO board 0(Ref. Chapters 3 and 9)

25. What assemblies are located on the system CAN-BUS line?

The CU board, OPTIBO board, diluters, and LiHa are on the system CAN-BUS line. (Ref. Chapters 3 and 9)


26. What assemblies are located on the Option BUS line?

The CU board, OPTIBO board, PosID, RoMa, MPO, SMIO board, SMIO-SAFY board, and CC Mags boards #3 and #4 are on the Option BUS line. (Ref. Chapters 3 and 9)

27. What do fast blinking red and green lights on the CU board indicate?

Fast blinking red and green lights on the CU board indicate a firmware download is required. (Ref. Chapter 3)

28. To what connection on the OPTIBO board should the cable from the 50°C heating block of the 1 ml Subsystem be plugged in?

The cable is plugged in to either CAN OPT 3 or 4 on the OPTIBO board.(Ref. Chapters 9.2.1 and 9.12.5)

29. When the S&S software is being installed, what options should be selected in the Select Components screen?

Worktable Editor, Instrument, System Devices (Move Test, RoMa, LiHa), and NAT software should be selected. (Ref. Chapters 4 and 13.1.1)

30. What is the field service password for the S&S software?

The field service password is “smart”.

31. Which worktable is used for the m1000?

The NAT MBS Worktable is used for the m1000. (Ref. Chapter 13.5.5)

32. Which two buttons in the S&S software are used the same way to initialize the instrument?

The instrument is initialized using menu ‘System/Initialize Instrument’ or using the INIT Quick access button on the toolbar. (Ref. Chapters 13.4.3 and 13.5)

33. During the Tip Adapter Test for the LiHa, the FSR observes the progress bar which indicates the tip was detected but the test fails. Why?

The Tip Adapter was not held up in position when the [Next] key was pressed.

34. Why is the LiHa right reference position 40 and not 45?

The LiHa cannot move past the RoMa.

35. Why is the RoMa left reference position 7, not 1?

The RoMa cannot move past the LiHa.



36. What rotator type, gripper type, and Z-range are used on the m1000 RoMa?

The RoMa uses CW rotator type, Eccentric gripper type and Extended (long) Z-range. (Ref. Chapter 4)

37. When testing the system CAN-BUS line to determine if the correct number of jumpers are installed, the resistance should be approximately _________ Ohms.

50 to 65 Ohms should be detected. (Ref. Chapters 4 and 9)

38. How is the functionality of diluter #6 verified?

Use the ‘LiHa/Diluter’ tab. Select Slider 6, select the [Init] key, then lower the slider down to a desired volume. (Ref. Chapter 13.13.13)

39. How is the connection of diluter #2 to LiHa axis #2 confirmed?

Use the ‘LiHa/Diluter’ tab. Select Slider 2, select the [Init] key, then lower the slider down to a desired volume. Verify diluter #2 syringe is moving and liquid is being dispensed from LiHa axis #2. (Ref. Chapter 13.13.13)

40. There are three ways to fill/flush the liquid system. List two of the three ways.

1) Use the LiHa Diluter tab, Liquid Detection menu/[Fill/Flush] key2) Use the FaWa [Fill/Flush] key3)

41. How should a backup of the EEPROM parameters of the LiHa be performed?

To perform backup of EEPROM parameters: select ‘Basic Setup’/‘Contents’ tab. Select the ‘EEPROM Backup/Restore’ menu item.Go to the ‘EEPROM’ tab. Select the M1 (where settings of LiHa are stored) device(s) to be restored then select [Start]. (Ref. Chapter 13.7.4)

42. What should be checked if a liquid bottle sensor is failing? If multiple sensors are failing?

If a single sensor is failing, check:• liquid is not present on the walls of the bottle• bottle is flush against sensor• sensor is connected at the SMIO board• sensor is functioning, by using the S&S software

If multiple sensors are failing, check:• fuse F1• cables running to the SMIO-SAFY and OPTIBO boards• address and J9 jumper are set correctly(Ref. Chapters 7, 9, 12, and 14)


43. One heater on the 1 ml subsystem has failed. What should be checked?

The following should be checked:• Fuses FU8 and FU9• Cable connections OPT 3 and 4 on the OPTIBO board• Cable connections at the CC Mags boards• Cable connections at the heater block• Use single line commands to determine if the heater warms up to temperature(Ref. Chapters 3, 7, 9, and 14)

44. On the pressure relief valve, to what level should the pressure be adjusted?

The pressure on the pressure relief valve must never be adjusted.

45. An FSR replaced a failed diluter and the diluter still does not work. What is a possible reason for the failure?

There is a high probability the address is set wrong or the diluter is not seated into the VCC Dilback board.

46. An FSR replaced a RoMa and now it rotates CCW instead of CW. What could be wrong?

Two areas that should be inspected include the set screws on the RoMa rotator and the configuration defined in the RoMa/Setup page. (Ref. Chapters 3, 4, and 13)

47. Two LiHa axes, Z7 and Z2, are not functioning. Which Servo board controls these two boards?

The DC Servo board located in position 3 (from the top), with the address set to 2. (Ref. Chapter 9)

48. List the order of insertion of the black O-ring and white separator ring, when the two rings on the DiTi Adapter are replaced.

The white plastic O-ring is inserted first, followed by the black rubber O-ring. (Ref. Chapter 9)

49. To what connector on the Tip Adapter board should the ILID cable be connected?

Connector J1 on the Tip Adapter board. (Ref. Chapter 9.6.3)

50. What functions do the RoMa Freedom Backplane and the RoMa 2 Backplane control?

The two boards control the X, Y, Z, R, and G movements of the RoMa. (Ref. Chapter 9)

51. Where is the EEPROM for the RoMa located?

The EEPROM for the RoMa is located on the Device CU board which is plugged into the RoMa Freedom Backplane board. (Ref. Chapter 9.7.17)



52. Outline the flow/route of liquid from the system liquid bottle to the DiTis.

System liquid bottle � Aspiration tubing � FaWa pump �Y-connector (that goes back to either waste or system bottle) � Aspiration tubing �Y-connector � 1:4 Distributor � Interconnecting tubing �Diluters � 3-way valve � Syringes �Valves � Pipetting tubing � DiTi Adapter �DiTis � Wash/waste station � Liquid waste. (Ref. Chapter 9.9.2)

53. To what connector(s) on the SMIO-SAFY board is the left door lock connected?

The left door lock is connected to J5 and J24 on the SMIO-SAFY board. (Ref. Chapter 12)

54. What is the protocol to verify that heater zone 1 is heating to 50°C ± 2.5°C?

Turn on the heater zone 1 using the single line command O4STT500 in the command line. The answer should be 04@. Wait 20 minutes for the heater block to equilibrate. Type 04RAT in the command line and select [Transmit] to read the actual temperature. The values should be between 475 and 525. (Ref. Chapter 9.12.1)

55. How is firmware downloaded?

From the ‘Basic Setup’ panel select ‘Firmware Download”. Change to the Firmware Download page. Select the corresponding ‘Do for’ checkbox of the device firmware to be downloaded, then select [Start]. (Ref. Chapter 13.7.3)

56. If the machine size of the m1000 is not set to 150, what should be done?

On the ‘Contents’ page of the ‘Instrument Basic Setup’ panel, select ‘Set Machine Size’ then press [Start]. (Ref. Chapter 13.7.7)

57. If the instrument type is not Freedom, what should be done?

On the ‘Contents’ page of the ‘Instrument Basic Setup’ panel, select ‘Set X-Drive Properties’ then press [Start]. All arm setups should be done after this step. (Ref. Chapter 13.7.6)

58. What is the change position of the PosID?

The change position is the transition position in which the scanner head goes over from moving around its vertical B’-axis to moving around its horizontal X’-axis or vice versa. (Ref. Chapter 13.12.2)

59. What test is performed to determine if tips are picked up and disposed of correctly?

The ‘Lower DiTi Eject Test’ is used to determine if tips are picked up and disposed of correctly. (Ref. Chapters 4 and 13.18.1)


60. What test is performed to verify that liquid is sensed and detected consistently and accurately?

The ‘Liquid Detection Test’ is used to verify liquid is sensed and detected consistently and accurately. (Ref. Chapters 4.5 and 13.19)

61. An FSR replaced a FaWa pump assembly and the pump does not work. What is the problem?

FaWa Setup was not performed. The number of pumps was not set to 1 and the information was not stored on EEPROM. (Ref. Chapters 4.5 and 13.20.1)

Documents

69528-101 m1000 Service Training Guidegss.pmh.com.br:9010/moleculardiagnostics_cd/69528101.pdf · Page 2 m1000™ Service Training Guide 69528-101—September 2003 Proprietary Information