PATENT ABSTRACTS 287

5 3 5 4 6 6 8

M E T H O D S F O R T H E I S O T H E R M A L

A M P L I F I C A T I O N O F N U C L E I C A C I D M O L E C U L E S

Jeffrey I Auerbach

lagen chain in solutions and other human tissue specimens using immunolo)~'c~l methods com- prised of antibodies specxfic for satd protein. Along this line, the invention relates to the use of the specific antibodies to examine the presence or absence of the alpha5(IV) collagen chain in the tissues, e.g. skin or kidneys, etc. of patients with renal failure, possibly due to Alpart's syndrome.

Methods for amplifying a nucleic acid molecule which employs a single primer, and in which the ampfification is performed under isothermal conditions. The invention also includes kits containing reagents for conducting the method.

5 3 5 4 6 7 9

M I C R O O R G A N I S M I M M O B I L I Z A T I O N I N A B E T A -

C H I T I N C A R R I E R

Eiji Ohashi, Tokyo, Japan assigned to Nippon Snisan Kalsha Ltd

A suspension of a microorganism or a plurality of different microorganisms is added to a dispersion of beta-chitin gel, and the resultant dispersion is molded and dried to produce an immobilized microorganism such as a film or membrane containing the microorganism. The beta-chitin gel dispersion is prepared by iso- lating and grinding beta-chitin from a source such as cuttlefish pen or squid pen, dispersing the ground beta-chitin in water and forming a gel dispersion. In forming the gel dispersion, the dispersion of ground beta-chitin is gelled and the gel is dispersed in water. Freeze-thawing may be used in gelling the dispersion of ground betachitin. A film of the immobilized micro- organism can be formed on an electrode to prepare a microorganism electrode.

5354846

S T R E P T O L Y S I N O A N T I G E N D E R I V A T I V E S , I T S

P R O D U C T I O N A N D U S E S

Michael Kehoe, Ebchester, County Durham, United Kingdom

A non-toxic and non-cytolytic derivative of streptolysin 0 which retains at least one

referably immunodominant) epitope is pro- ced, and can be used especially in diagnostic

tests for detecting of the presence of antibodies to Streptococcus pyogenes in a sample.

5354852

P O L Y S A C C H A R I D E D E R I V A T I V E , P R O C E S S F O R

P R O D U C I N G T H E S A M E , A N D S E P A R A T I N G A G E N T

Hirokaz Ikeda, Hyogo, Japan assigned to Daicel Chemical Industries Ltd

5354690

I M M U N O L O G I C A L M E T H O D S F O R T H E D E T E C T I O N O F T H E H U M A N T Y P E I V C O L L A G E N

A L P H A 5 C H A I N

Kar Tryggvason, Sirkka Hostikka, Hoyhtya- Mat t i , SF 90570 Oulu, Finland

The present invention provides for a method for preparing antibodies that specifically detect the .type .IV collagen alpha5 chain that is defective m various basement membrane disorders such as in X chromosome~linked Alport's syndrome. The invention further provides for the use of such antibodies to detect the alpha5(IV) col-

A particulate aromatic or araliphatic carbamate of a polysaccharide having a mean particle di~tmeter of I to 200 mum and a specific surface area of 0.5 to 300 m2/g is prepared by first dissolving an aromatic or araliphatic carbamate of a polysaccharlde in an organic solvent, adding a hydrocarbon having 4 to 22 carbon atoms in an amount of 0 to 0.5 time by volume of that of the organic solvent, gradually adding the resultant solution to a sufficiently agitating aqueous surfactant solution, removing the organic solvent while continuing the agitation, isolating solid particles, and washing and drying the sofid particles. The particulate aromatic or araliphatic carbamate of a polysaccharide has an excellent capability of resolution and, as such, can be used as a stationary phase of ~ . r ~ t i v e liquid chromatography without

supported on a support.