484 PATENT ABSTRACTS

reaction takes place between a selected oxidant, an enzyme for catalyzing oxidation reactions of that oxidant, and a substrate for that enzyme, resulting in an intermediate product which is a sufficiently-powerful oxidizing agent to bring about luminescent oxidation of a chemilumines- cent DPD. In the second step, that intermediate compound is reacted with DPD resulting in a brief and relatively intense emission of light. An assay kit to facilitate the carrying out of this as- say procedure.

5115099

SUBSTRATES FOR DETERMINATION OF ENZYME

ACTIVITY AND INTERMEDIATES FOR SYNTHESIS OF THE

SUBSTRATES AS WELL AS PROCESS FOR P R O D U C I N G THE

INTERMEDIATES

Katsumas Kuroiwa, Hitoshi Matsuura, Kat- suhiro Katayama, Shuichi Nakatsuyama, Takeshi Nagasawa, Koji Endo, Koriyama, Japan assigned to Nitto Boseki Co Ltd

5114846

PROCESS FOR P R O D U C I N G STREPTOVARICIN

Kaname Inoue, Motohide Yamazaki, Richard W Armentrout, Kawasaki, Japan assigned to Shin-Etsu Bio lnc

A method for selecting hyper-producing strains of streptovaricin C by culturing streptomyces spectabilis and separating the asporogenous colonies. The asporogenous colonies are then separately cultured and tested for streptovaricin productivity. Those colonies having the highest productivity may then be easily selected.

Novel compounds represented by the following formula: See Patent for Chemical Structure wherein A represents a specific amino acid residue are excellent as substrates for determina- tion of enzyme activity such as trypsin, etc. The compounds can be synthesized from novel arginyl-3-tert- alkyloxycarb onyl-4- nitroanilides by a novel process comprising a selective deprotection s t e p whereby the pro- tecting group on the alpha-amine group of ar- ginine is r c m o v e d in the presence of a hydrochloric acid, acetic acid and dimethylfor- mamide m i x t u r e , but a tert-alkyl protecting group on the -COOH group of the nitroaniti de ring is not removed by this step.

5116728

REAGENTS FOR CO2 DETECTION

Fredric H Crowther, Wai T Law assigned to Em Diagnostic Systems Incorporated

5114858

CELLULAR C O M P O N E N T EXTRACTION PROCESS IN A

DISPOSABLE FILTRATION VESSEL

John G Williams, Louis Rosanio assigned to E 1 Du Pont de Nemours and Company

A stabilizer tbr maintaining a constant level of CO2 in coenzyme containing enzymatic CO2 diagnostic reagents is disclosed, comprising rate- limiting amounts of (a) the diagnostic reagents and (b) coenzyme-regenerating reagents, e.g., wherein {a) is malate dehydrogenase, phosphoenolpyruvate carboxylase and NADH, and (b) is glucose dehydrogenase and glucose, as are methods of use and kits containing a dia- gnostic reagent and stabilizer.

A method of extracting cellular components, e.g., nucleic acids, particularly DNA, from bio- logical, expecially solid tissue, samples, par- ticularly from plants is provided, whereby all of the steps can be performed in one vessel, and the entire process can be automated. A vessel suitable for this process is provided, as well as a system for the automated performance of the process steps.

5116729

STABILIZATION OF OXIDASE ENZYME-BASED TEST STRIPS

Ibrahim lsmail, Wen H Wu assigned to Miles Inc

A method and composition for the enzymatic as-

PATENT ABSTRACTS 485

say of liquid test sample, such as urine or serum, for a specific analyte, such as glucose. The method utilizes a dry phase test strip comprising a carrier matrix impregnated with a stabilized enzyme reagent composition including an ox- idase enzyme, such as glucose oxidase, that is capable of interacting with the analyte of inter- est; a chromogen that changes color in response to the analyte-oxidase enzyme interaction to reveal the presence and/or concentration of the analyte in the test sample; and a stabilizer, such as a hydrazide, like GIRARD'S Reagent T, or an amino acid, like arginine, or an amino acid derivative, like the tris(hydrox- ymethyl)aminomethane salt of glutamic acid, to improve the heat stability and shelf life of the dry phase test strip.

formed by oxidation ofa chromogenic substrate in the presence of the catalyst, together with two or more co-precipitated metals. A strong signal is formed with which to detect an oxidation catalyst which is localized to a target molecule. Target molecules may be nucleic acids, anti- bodies or cell surface antigens.

5116747

I M M O B I L I Z A T I O N O F B I O L O G I C A L L Y A C T I V E

M A T E R I A L I N C A P S U L E S P R E P A R E D F R O M A WATER-

SOLUBLE P O L Y M E R A N D C H I T O S A N A C E T A T E

5116730

A M I N O A C I D S U B S T I T U T E D 4 - A M I N O P H E N A Z O N E S F O R

M E A S U R I N G E N Z Y M E A C T I V I T Y

Joseph Artiss, Jill M Bensie, Benni Zak, Daniel Raden, Windsor, Ontario, MI, Canada assigned to Board of Governors of Wayne State Univer- sity

Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4- aminophenazone reacts with the enzyme to pro- duce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is par- ticularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-bydroxy-3, 5- dichlorobenzenesulfoaate, to form a red chro mogen in the presence of an oxidizing agent, such as bilirubin oxidase.

5116734

H I G H L Y S E N S I T I V E M E T H O D F O R D E T E C T I N G P E R O X I D A S E

Thomas W Higgs, Floyd Taub assigned to Digene Diagnostics Inc

The present invention is directed to a composi- tion of matter and a process for detecting the pre- sence of an oxidative catalyst in a biological sample. The composition comprises a precipitate

Murray Moo-Young, Niels C Bols, Sandra E Overgaard, Jeno Scharer, Wa terloo, Canada as- signed to University of Waterloo

Biologically-active material, particularly hybridoma cells, is immobilized for mass culture in bioreactors to produce biological products, particularly monoclonai antibodies, by ionically=interacting polycationic groups on chitosan with polyanionic groups on a poly- anionic water-soluble polymer. Preferably, the chitosan is chitosan acetate and the polyanionic polymer is sodium alginate. In one embodiment, droplets of a suspension of the biologically- active material and water-soluble polymer are gelled by forming a calcium salt of the water- soluble polymer to form temporary capsules, and a sequestering agent for the calcium ions is added to effect ionotropic gelation of the water- soluble polymer and chitosan to form a semi- permeable membrane around each temporary capsule. In another embodiment, porous beads are formed by exposing droplets of a suspension of the biologically active material and water- soluble polymer to an aqueous solution of chitosan and multiple cations. In a further em- bodiment, chitosan is added to a suspension of the biologically active material and water- soluble polymer and the resulting suspension is extruded into a solution of multi-valent anions to form a fibrous mass.

5116751

P R O C E S S F O R P R O D U C I N G P E R O X I D A S E

Yoshifumi Shinmen, Sumio Asami, Norihide Amano, Teruo Amachi, Hajime Yoshizumi, Kyoto, Japan assigned to Suntory Limited