PATENT ABSTRACTS 119

5043276

D N A S T R A N D C O D I N G F O R A L P H A - A C E T O L A C T A T E

D E C A R B O X Y L A S E A N D Y E A S T T R A N S F O R M E D W I T H T H E D N A

S T R A N D

a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

Shigeyuk Yamano, Junichi Tanaka, Takashi In- oue, Takasaki, Japan assigned to Kirin Beer Kabushiki Kaisha

DNA strand having an ability in bio- technological production of alpha-acetolactate decarboxylase is disclosed. The DNA strand is characterized in that it has a nucleotide sequence coding for a polypeptide whose amino acid sequence is substantially from A to B of FIG. 1 and which has alpha-acetolactate deca- rboxcylase activity. Also disclosed is a yeast which belongs a Saccharomyces cerevisiae and which has been transformed by the DNA strand. The yeast is characterized by the fact that its alpha-acetolactate producing ability is reduced, and wilt thus produce an alcoholic liquor such as beer which contains no or little diacetyls which have come from their precursor, namely alpha- acetolactate.

5043279

D N A E N C O D I N G A B A C I L L U S C R E A T I N A S E

Hitoshi Sagai, Harumi Masujima, Shigeru Ikuta, Koji Suzuki, Mishima, Japan assigned to Toyo Jozo Company Ltd

The disclosure is made on a poly- deoxyribonucleic acid having a base sequence encoding an amino acid sequence of a poly- peptide which is a creatinase derived from a creatinase-producing microorganism belonging to the genus of Bacillus. The bsae sequence of the creatinase gene DNA and amino acid sequence of the creatinase have been clarified. The trans- formant having this polydeoxydbonucleic acid as well as a process for preparing the creatinase at a high productivity with the application of genetic engineering technique are disclosed.

5043277

M E T H O D O F R E G U L A T I N G E X P R E S S I O N O F A F O R E I G N

G E N E BY C O N T R O L L I N G C U L T U R E T E M P E R A T U R E A N D A

P R O C E S S O F P R O D U C I N G A F O R E I G N G E N E P R O D U C T

T H E R E B Y

Nobuhiro Fukuhara, Setsuo Yoshino, Kaoru Yamamoto, Satori Sone, Maki Suzuki, Yoshiyuk Nakajima, Ohmuta, Japan assigned to Mitsui Toatsu Chemicals Incorporated

Eseherichia colt carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40 degrees C. or over so that the expression of said desired foreign gene was suppressed. This E. colt (i.e., transformant) was cultured at 40 degrees C. or over in a first process to suppress the expres- sion of the foreign gene and to support sufficient cell growth and thereafter below 40 degrees C. in

5045313

V A C C I N E F O R I M M U N I Z I N G C A T S A G A I N S T T O X O P L A S M A

O O C Y S T S H E D D I N G

Jacob K Frenkel, Elmer R Pfefferkorn assigned to The University of Kansas; Darmouth Colle

A new mutant strain vaccine, and a correspon- ding method of immunization, against Tox- oplasma in cats is provided which makes use of a reproductively deficient strain of T.gondii, designated T-263. Bradyzoites in tissue cysts from laboratory animals infected with the mutant were fed to cats, which developed im- munity against subsequent T.gondii challenge without concomitant shedding of infectious oocysts. The new vaccine eliminates the need for chemoprophylaxis subsequent to primary infec- tion.