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4th International Symposium on

Plant Apoplastic Diffusion Barriers

28 – 30 August 2019

Bonn, Germany

PROGRAMME BOOK Local Organizer and Symposium Chairman

Dr. Rochus B. Franke Institute of Cellular and Molecular Botany, University of Bonn, Germany Scientif ic Advisory Committee

Dr. Christiane Nawrath Université de Lausanne, Switzerland Dr. Katja Arand Julius-Maximilians-Universität Würzburg, Germany Dr. Frédéric Domergue Université de Bordeaux, France Prof. José Graça Universidade de Lisboa, Portugal

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Welcome to the 4th Symposium on Plant Apoplastic Diffusion Barriers

Dear friends and colleagues, It is a great pleasure to welcome you to the 4th PADiBa Symposium, the City of Bonn and the Hotel Collegium Leoninum. Together with the Scientific Committee we hope and believe that you will enjoy the three busy days of talks, two days of poster presentations and a relaxing conference dinner. With almost 70 participants from 16 countries, the symposium highlights six invited speakers, 25 lectures and 30 posters. I am also pleased to welcome a substantial delegation of students and early career investigators that to me looks like the new generation of PADiBa scientists that will ensure the continued advancement of research in this exciting field of plant science. Please also find the time to enjoy the city and the surroundings. The riverbanks and city centre - with historic buildings, Münster, museums, beer garden and cafes - are best explored on foot. A short tram ride south takes you to the “museums mile” adjacent to the former governmental area. I would also like to take this opportunity to thank the international committee for their help in evaluating submitted abstracts and in building the strong scientific program. I am also grateful for the help and support from the local student support team. Please feel free to approach these people with any queries or issues. Great appreciation is due as well to the German Research Foundation for their financial support. I wish all of us a fruitful, meaningful and pleasant meeting. Rochus Benni Franke (Chair)

Organizer PADiBa 2019

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Organizer Local Organizer and Symposium Chairman

Dr. Rochus B. Franke Institute of Cellular and Molecular Botany, University of Bonn, Germany

Scientific Advisory Committee

Dr. Christiane Nawrath Département de Biologie Moléculaire Végétale, Université de Lausanne, Switzerland

Dr. Katja Arand Julius-von-Sachs-Institute for Biosciences, Julius-Maximilians-Universität Würzburg, Germany

Dr. Frédéric Domergue Laboratoire de Biogenèse Membranaire, Université Victor Segalen Bordeaux, France

Prof. José Graça Instituto Superior de Agronomia, Universidade de Lisboa, Portugal

Organizer PADiBa 2019

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Local Support Team

Mr Falko Ly Bachelor Candidate, Institute of Cellular and Molecular Botany, University of Bonn, Germany

Ms Lisa Zander Master Candidate, Institute of Cellular and Molecular Botany, University of Bonn, Germany

Mr Kiran Suresh Master Candidate, Institute of Cellular and Molecular Botany, University of Bonn, Germany

General Information PADiBa 2019

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General Information MEETING VENUE Hotel Collegium Leoninum Noeggerathstraße 34 D-53111 Bonn, Germany www.leoninum-bonn.de INTERNET ACCESS WiFi is provided by the hotel. WiFi token to connect to this network will be available at the registration desk. FACILITIES The toilets are located in the basement. NAME BADGES All participants should wear their name badge in the meeting area at all times. LANGUAGE Most Germans speak English and many signposts in Bonn are also in English. The official language of the symposium is English. LUNCH & COFFEE BREAKS Lunch and coffee breaks are included in the registration fee for all participants. MOBILE PHONES As a courtesy to other delegates, please ensure mobile phones are turned of or are in silent mode during all sessions and social program. DRESS CODE Dress will be informal throughout the symposium. SMOKING Smoking is not allowed in indoor public places, restaurants and pubs in Bonn. Thus the meeting venue is a no-smoking place and smoking is permitted only outside the venue. USEFUL PHONE NUMBERS Emergency number 112 Hotel Collegium Leoninum +49 228 62 98 492 Taxi +49 228 55 55 55 Getting around in Bonn Bus /Tram https://en.swb-busundbahn.de Scooter https://www.tier.app/?no_lredirect=true Bikes https://www.nextbike.de/en/bonn/


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City map

Venue map


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How to get to the conference dinner?

The MS Moby Dick - the boat where we have the dinner - is waiting on the quay in Brasser Ufer, below the Collegium Albertinum Church. Boarding for the cruise starts shortly after 18:00h. The Moby Dick departs is at 18:30h and will return at 22:30h.


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It is 15 – 20 minutes walks from the Hotel Collegium Leoninum to the Moby Dick. The walk is across the city centre, mostly in the pedestrian zone, passing the historic university main building and a small park. https://goo.gl/maps/kQeM63adEsvjzbKW7 From the Motel One it is only about 10 minutes walk along the river. First cross the main street and than turn left to the riverbanks. https://goo.gl/maps/bQWgtsW5BhY1TnF27 We will have guided walks departing 17:45h from the Hotel Collegium Leoninum and 17:50 from the Motel One.


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Food outside the symposium venue You will not suffer from hunger at the symposium site. However there might be some need for food or drinks during the evening or an extended stay in Bonn. Below is a list of nearby locations summarized in the online map linked below: https://drive.google.com/open?id=1Nrqc7dD-slECAoYLjPXHtTIH8yVS9Cwj&usp=sharing Beer Garden (outside seating, near the river, also some food) Biergarten Schänzchen – Bavarian stile Biergarten Am alten Zoll – nice spot under a giant plane tree Rheinlust – on the sunny side of the river Bahnhöfchen – on the sunny side of the river Biergarten "Zum Blauen Affen" – run by rowers Brewpubs and German food Brauhaus Bönnsch – the local version of Kölsch Bierhaus Machold – Schnitzel, Sausage, Sauerbraten, Beer Restaurant Sudhaus - Schnitzel, Sausage, Sauerbraten, Beer Restaurant Zum Treppchen – one of the oldest in town, Bacon and beans Gasthaus Im Stiefel – traditional Kölsch pub Em Höttche – traditional Kölsch pub near the old Rathaus Bonnum – Schnitzel of course International Food Tuscolo Frankenbad – since years highly ranked for best pizza in town Tuscolo Münsterblick – a newer branch of the pizza king Tacos Bonn – Mexican and cocktails (almost always happy hour) Serhat – original Turkish Der Spanier – a supermarket that turns in a bistro in the evening, get drinks from the shelf (garlic-ish) San Telmo – Spanish, informal seating Mandu – bowls and other Korean food Cala-Dor Pizza – cult pizza for late night Ice Cream EisLabor Eismanufaktur Solo Qui Bonn Just Drinking and mix in with the young Bonns Das Nyx – difficult name Bla – need one m2 of tattoos minimum to be a waiter The Quiet Man – British pub Dubliner Irish Pub Bonn Café Pawlow – inside and outside drinking for older than teens Die Wache – after midnight only when others are closed already Old Jacob – Cocktails from Bartender of the Year Sembo Amirpour Motel One – Large Gin selection Steinbeck – potentially hip


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Information for speakers Time allocated to talks The time available for each regular presentation is limited to 20 minutes, including a 5 minutes question period. The time available for PhD student presentations is limited to 15 minutes, including a 3 minutes question period. Session chairs will strictly enforce presentation times. Presentation format Presentations will be in English. Presentations should be prepared using Microsoft PowerPoint or PDF applications in 16:9 landscape formats. Please make sure that your presentation - including movies and fonts - will run correctly on a PC with Windows10 and Office 2016 installed. Presentation upload Presentations should be provided on an USB drive for uploading on the conference computer. Choose a file name beginning with your last name (e.g. “Smith_lignosuberocutax.pptx”). Please contact media staff member in the conference room to upload your presentation – either in the morning before the programme starts (all 1st day speakers) or at the beginning of the poster sessions, lunch or coffee breaks (2nd and 3rd day speakers).

Information for poster presenters Poster sessions Poster sessions take place in the “KAPITELSAAL” on the second floor. Poster sessions are held on Wednesday 28 August 16:40h – 19:00h and on Thursday 29 August during coffee breaks (10:10h -10:40h & 15:05h -16:00h). Authors are expected to be present at their poster during the poster session. Poster mounting and removal Posters can be mounted from Wednesday 28 August, from 08:30h and should be installed by the first coffee break at the latest. Posters must be removed after the last coffee break /poster session on Thursday 29 August.

Keynote Speaker PADiBa 2019

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Invited Speaker

Gabriel Castril lo

University of Nottingham, United Kingdom

Gwyneth Ingram

CNRS/UMR5667 ENS de Lyon, France

Concepcion Manzano

University of California Davis, USA

Yuree Lee

Seoul National University, Korea

Katja Arand

University of Würzburg, Germany

Yann Boursiac

BPMP, INRA, Montpellier, France

Programme Tuesday, 27 August 2019 PADiBa 2019

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Programme

Tuesday, 27 August 2019

18:30 – 21:00 Opening Mixer at Bonn University Botanic Garden Meet your collaborators, old and new colleagues and friends for an informal opening mixer held in the Mediterranean Greenhouse of the Bonn University Botanic Garden. For this welcome reception enter the Botanic Garden through the entrance in Meckenheimer Allee. Included in the registration fee. The Botanic Garden is about a 15-20 minutes walk from your hotel. For a nice walk along the Poppelsdorfer Allee start from the railway underpass. https://goo.gl/maps/T2bz9bYAdejq5SCE7

Scientific Programme Wednesday, 28 August 2019 PADiBa 2019

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Scientific Programme

Wednesday, 28 August 2019

08:30 – 10:00 Registration, Welcome Coffee (Vestibül) Postersetup (Kapitelsaal)

10:00 Welcome Address Rochus Benni Franke

Intitute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany

10:10 Introduction to Diffusion Barriers of the Apoplast in Plants Christiane Nawrath

DBMV, University of Lausanne, Lausanne, Switzerland

10:30 – 12:10 Block I (Old Church) – Chair Frédéric Domergue

10:30 – 11:10 Keynote presentation To the exodermis and beyond: what secrets does it hide?

Concepcion Manzano University of California Davis, USA

SESSION 1 – Biosynthesis, assembly and deposition of biomacromolecules in dermal tissues

11:10 Suberin and lignin - Where is the border? Tonni Andersen


11:30 A moss phenolic pathway controls cuticle formation and organ growth Hugues Renault

Institute of Plant Molecular Biology, CNRS, University of Strasbourg, France

11:50 Suberizing Potato Tubers when Time or Enzymes are in Short Supply: Biological and Bio-inspired Strategies as Viewed by Chemists Ruth E. Stark

CUNY City College of New York, New York, NY, USA

12:10 – 13:30 Lunch (Vestibül and Leo’s Bistro)

Scientific Programme Wednesday, 28 August 2019 PADiBa 2019

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13:30 – 14:50 Block II (Old Church) – Chair Bénédicte Bakan

13:30 – 14:10 Keynote presentation Cellular Architectures Controlling Organ Separation

Yuree Lee Seoul National University, Korea

SESSION 1 continuation

14:10 Cuticle Formation Requires The ABCG32 Transporter In Arabidopsis And Tomato Carolina Elejalde-Palmett


14:30 Elucidating the Role of BODYGUARD in Cuticle Formation Using Tomato (Solanum lycopersicum) Fruit as a Model Glenn Philippe

Cornell University, Ithaca, New York

14:50 – 15:20 Coffee Break (Vestibül)

15:20 – 14:50 Block III (Old Church) – Chair Marie Barberon

15:20 – 16:40 Keynote presentation Physiological Roles of Casparian Strips and Endodermal

Suberin in Salt Tolerance and Transport of Water and Solutes Yann Boursiac

BPMP, INRA, Montpellier, France

SESSION 2 – Structure and properties of cutinized, suberized and lignified cell walls

16:00 The role of the plant cuticle during apical hook development Sara Raggi

Umeå Plant Science Centre, Umeå, Sweden

16:20 The Role of Suberized Exodermis in Plant Nutrition and Growth Olga Serra

Universitat de Girona, Girona, Spain

16:40 – 19:00 Poster Session, Drinks & Snacks (Kapitelsaal)

Scientific Programme Thursday, 29 August 2019 PADiBa 2019

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Thursday, 29 August 2019

08:30 – 10:10 Block IV (Old Church) – Chair Katja Arand

08:30 – 09:10 Keynote presentation Subtilase mediated activation of a novel GSO1/GSO2 ligand

provides spatial information during embryonic cuticle integrity monitoring Gwyneth Ingram

CNRS/UMR5667 ENS de Lyon, France

SESSION 2 continuation

09:10 Root Cap Cuticle: Structure and Functions Alice Berhin


09:30 Cross-talk between the cutin polyester and cell wall polysaccharides Bénédicte BAKAN

INRA - Biopolymers Interactions Assemblies, Nantes, France

09:50 Aromatic Compounds in Cuticular Waxes: Biosynthesis, Localization, and Potential Function Reinhard Jetter

University of British Columbia, Vancouver, Canada

10:10 – 10:40 Conference Photo (Old Church) Coffee Break & Poster (Kapitelsaal)

10:40 – 12:20 Block V (Old Church) – Chair Tonni Grube Andersen

SESSION 3 – Regulation and development of cutinized, suberized and lignified cell walls

10:40 Grain to Hull Adhesion in Barley Sarah M McKim

University of Dundee, Dundee, Scotland

11:00 Suberin plasticity and function for nutrient transport Marie Barberon

University of Geneva, Geneva, Switzerland

11:20 Identification of Regulators for Increased Suberin Production Wolfgang Busch

Salk Institute for Biological Studies, La Jolla, USA

11:40 Modulating suberin deposition in the periderm: a MYB story Episode I Laura Ragni

ZMBP, University of Tübingen, Tübingen, Germany

12:00 SUBERMAN is a component of the regulatory network establishing endodermis suberization of the Arabidopsis root Hagai Cohen

Weizmann Institute of Science, Rehovot, Israel

Scientific Programme Thursday, 29 August 2019 PADiBa 2019

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13:40 – 15:05 Block VI (Old Church) – Chair Shahid Siddique

13:40 – 14:20 Keynote presentation Plant Microbiota Coordinate Root Diffusion Barriers and Ion

Homeostasis Gabriel Castrillo

University of Nottingham, United Kingdom

SESSION 4 Student Presentations

14:20 Modulating suberin deposition in the periderm: a MYB story Episode II David S. Molina Garcés

ZMBP, University of Tübingen, Tübingen, Germany

14:35 Genetic and histological studies reveal a close association between flavonoids and an endosperm cuticle in Arabidopsis seeds Lena Hyvärinen

University of Geneva, Geneva, Switzerland

14:50 Two kinases regulated by MYB36 are essential for correct localization of lignin in Casparian strip domain Qi Wu

The University of Tokyo, Tokyo, Japan

15:05 – 16:00 Coffee Break & Poster (Kapitelsaal)

16:00 – 16:40 Block VII (Old Church) – Chair Rochus Benni Franke

SESSION 5 – Mechanism and molecular factors underlying apoplastic barrier formation

16:00 Candidate genes involved in cork cambium activity and phellem differentiation highlighted by transcriptomic analysis Celia M Miguel

Faculdade de Ciencias da Universidade de Lisboa, Lisboa, Portugal

16:20 Suberin dynamics during lateral root formation Robertas Ursache


18:10 – 18:30 Meeting at the gate and boarding “Moby Dick”

18:30 – 22:30 Cruise and Conference Dinner on the MS Moby Dick

Scientific Programme Friday, 30 August 2019 PADiBa 2019

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Friday, 30 August 2019 09:00 – 10:20 Block VIII (Old Church) – Chair Penny von Wettstein-Knowles

09:00 – 09:40 Keynote presentation Ecophysiology Adaptations in Plant Cuticles - How do Cuticular

Waxes Contribute to Cuticular Barrier Properties? Katja Arand

Julius-Maximilians-Universität, Würzburg, Germany

SESSION 5 contiued

09:40 Walking a Waxy Path: Molecular Characterisation of Barley Eceriferum-yy Chiara Campoli

University of Dundee, Dundee, Scotland 10:00 Elucidating Exodermis - Suberization

Kaisa Kajala Utrecht University, Utrecht, The Netherlands

10:20 – 10:50 Coffee Break (Vestibül)

10:50 – 12:05 Block IX (Old Church) – Chair Christiane Nawrath

SESSION 6 – Diffusion barriers in biotic and abiotic plant-environment interactions

10:50 Cuticular wax esters contribute to drought stress in Arabidopsis thaliana Payal Patwari

Institute of Molecular Physiology and Biotechnology of Plants, Bonn, Germany

11:10 Root apoplastic barriers contribute to defence against plant-parasitic cyst and root-knot nematodes Shahid Siddique

University of California Davis, Davis, USA

11:30 Indoors and Open Air Analysis of a Dozen Genome Edited Tree Tobacco Lines Unveils the Significance of Cuticular Lipids in Physiology and Fitness Boaz Negin

Weizmann Institute of Science, Rehovot, Israel

11:50 Final Remarks, Evaluation, Closing Rochus Benni Franke

Intitute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany


PADiBa 2019

Mini Abstracts

Keynote Lectures

Keynote Lectures PADiBa 2019

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To the exodermis and beyond: what secrets does it hide? Concepción Manzanoa, Kaisa Kajalab, Lidor Shaar-Moshea, Alex Masona, Alex

Canto-Pastora, Mona Gourana, Robertas Ursachec, Carlos Del Pozod, Niko Geldnerc,

Siobhan M. Bradya aUniversity of California at Davis,Davis,USA; bUtrecht University,Utrecht,Netherlands; cLausanne University, Lausanne, Switzerland; dCBGP-INIA, Madrid,Spain.

Plant roots have developed mechanisms to control the entrance of solutes and water.

One of these mechanisms is the modification of the cell wall in specific root cell types

with polymers of lignin and suberin. We have aimed to investigate whether the

molecular mechanism controlling the exodermis differentiation are similar or distinct to

the endodermis using tomato as a model system.


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Cellular Architectures Controlling Organ Separation Yuree Lee School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

I will present our findings to show the cellular mechanism of localized cell wall

patterning in abscission zone discussing the relationship between cell type specificity

and cellular architecture. Understanding the coordination between the two cell types will

give a new insight to the abscission processes, which will be further applied to

understanding general mechanisms of cell wall processing.


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Physiological roles of Casparian strips and endodermal suberin in salt tolerance and transport of water and solutes

Monica Calvo-Polanco1, Zoe Ribeyre2, Myriam Dauzat2, Guilhem Reyt3, Christopher

Hidalgo-Shrestha4, Patrick Diehl4, Marc Frenger4, Rochus B Franke4, David E Salt3,

Thierry Simoneau2, Bertrand Muller2, Christophe Maurel1, Yann Boursiac1 1 BPMP, Univ Montpellier, CNRS, INRA, SupAgro, Montpellier, France. 2 LEPSE, Univ Montpellier, INRA, SupAgro, Montpellier, France. 3 Future Food Beacon of Excellence and the School of Biosciences, University of Nottingham, LE12 5RD, UK. 4 Institute of Cellular and Molecular Botany, University of Bonn, 53115 Bonn, Germany

We use a combination of physiology, molecular biology and modelling to understand the

role of the apoplastic barriers in the transport of water and solutes through roots.


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Subtilase mediated activation of a novel GSO1/GSO2 ligand provides spatial information during embryonic cuticle integrity monitoring

N. Doll1, S. Royek2, S. Fujita3, S. Okuda4, A. Stintzi2, M. Hothorn4, A. Schaller2,

N. Geldner3, Gwyneth Ingram1 1 Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS de Lyon, UCB Lyon, CNRS, INRA, F-69342, Lyon, France. 2 Institute of Plant Physiology and Biotechnology, University of Hohenheim, Stuttgart, Germany. 3 Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland. 4 Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland.

Plants in the terrestrial environment must effectively limit water loss. In Arabidopsis the

first organs to emerge upon germination, the cotyledons, are covered by a functional

cuticle. We study the molecular dialog between the embryo and surrounding endosperm

that allows the establishment of a continuous embryonic cuticle during seed

development, prior to its deployment upon germination


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Characterisation of the regulatory interplay between the plant microbiome and root endodermal cell wall diffusion barriers

Guilhem Reyta, Isai Salas-Gonzalezb, Paulina Flisa, Valeria Custodioc, David E Salta and

Gabriel Castrilloa. a Future Food Beacon of Excellence and the School of Biosciences, University of Nottingham, Sutton -Bonington, United Kingdom; b Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America; c Instituto de Tecnologia Quimica e Biologica/Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, Portugal

Plant-associated bacteria are crucial in host adaptation to environmental stresses.

Beneficial microbes increase nutrient accumulation in the host, improving plant health. It

is unknown how the plant microbiota and the ions diffusion barrier at the root coordinate

to control the flow of nutrients. Our work provides evidence of this coordination in the

control of the leaf ionome.


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Ecophysiology Adaptations in Plant Cuticles – How do Cuticular Waxes Contribute to Cuticular Barrier Properties?

Katja Aranda, Amauri Buenoa, Ann-Christin Deiningera, Jana Leidea, Pascal Seuferta,

Simona Staigera and Markus Riederera aJulius von Sachs Institute of Biological Sciences, Chair of Botany II, University of Würzburg, Würzburg, Germany

The plant cuticle is physico-chemically designed to minimize transpiration. Contrary to expectations, the barrier properties are not correlated to the amount of waxes. With respect to the chain length and functional groups, we showed that the composition of the very-long-chain aliphatics coincides with different plant life strategies to cope with environmental factors like drought and temperature.

Kickoff Lecture PADiBa 2019

Introduction to Diffusion Barriers of the Apoplast in Plants Christiane Nawrath Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland

Introduction to the main diffusion barriers of the apoplast: cuticle, suberin lamellae and

Casparian strip. Overview of their extracellular localization, structure, composition and

function. The key steps of the biosynthetic pathways of the components: cutin, cuticular

wax, suberin and lignin are given.

SESSION 1

Biosynthesis, assembly and deposition

of biomacromolecules in dermal tissues

Biosynthesis, assembly and deposition of biomacromolecules in dermal tissues PADiBa 2019

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Suberin and lignin - Where is the border? Tonni G. Andersenab Laura Ragnic and Niko Geldnera aUniversity of Lausanne, Lausanne, Switzerland; bMax Planck Institute for Plant Breeding Research, Cologne, Germany; cUniversity of Tübingen, Tübingen, Germany.

We here show that lignification and suberization in the root barriers occur cell autonomously. Intriguingly, we find that deployment of suberin is highly dynamic and dependent on local synthesis of phenylpropanoids. Our findings highlight importance of local production of compounds in barrier formation and provides genetic tools applicable to manipulate this in a variety of tissues.


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A moss phenolic pathway controls cuticle formation and organ growth

Lucie Kriegshausera, Samuel Knospa, Desirée Gütleb, Trevor H. Yeatsc, Laurence

Herrgotta, Matthieu Erhardt a, Jocelyn K.C. Rose c, Ralf Reskib,d, Danièle Werck a and

Hugues Renaulta a Institute of Plant Molecular Biology, CNRS, University of Strasbourg, France; b Plant Biotechnology, Faculty of Biology, University of Freiburg, Germany; c Department of Plant Biology, Cornell University, Ithaca, USA; d BIOSS Centre for Biological Signaling Studies, University of Freiburg, Germany

Land colonization by plants was a major evolutionary event that paved the way for the

establishment of rich terrestrial ecosystems. Yet, the key early adaptations that allowed

plants to adapt to lands remain unclarified. In this context, we question the ancestral

functions of the plant-specific phenylpropanoid pathway that produces apoplastic

biopolymer precursors using bryophyte models.


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Suberizing Potato Tubers when Time or Enzymes are in Short Supply: Biological and Bio-inspired Strategies as Viewed by Chemists

Keyvan Dastmalchia, Mathiu Perez Rodrigueza, Arina Kligmana,b, Janni Lina,

Stephan Smitha, Barney Yooc, Liqing Jina,b, Qing Caia,b, Wenlin Huanga,

Mercè Figuerasd, Olga Serrad, Ruth E. Starka,b a Department of Chemistry and Biochemistry, CUNY City College of New York and Institute for Macromolecular Assemblies, New York, NY 10031, USA; b Ph.D. Programs in Chemistry and Biochemistry, CUNY Graduate Center, New York, NY 10016, USA; c Department of Chemistry, CUNY Hunter College, New York, NY 10065, USA; d Laboratori del Suro, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, E-17071, SPAIN

The protections offered by potato tuber suberization require formation of a

macromolecular composite embedded within the polysaccharide cell wall. These

essential defenses typically develop during tuber growth or alternatively after wounding

of the skin. What work-arounds are available if a rapid response to wounding is

required or enzymes involved in biosynthesis are inactive or unavailable?


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Cuticle Formation Requires The ABCG32 Transporter In Arabidopsis And Tomato

Elejalde, Caro1, Geisler, M3, Mazurek, S4, de Bellis, D2, Mucciolo, A2, Clausen, MH5,

Aharoni, A6, Schreiber, L7, Weder, C8, Bakan, B9, Stark, R10 and Nawrath, C1 1 University of Lausanne, DBMV, Lausanne, Switzerland; 2 University of Lausanne, EMF, Lausanne, Switzerland; 3 University of Fribourg, Department of Biology, Fribourg, Switzerland; 4 University of Wroclaw, Department of Chemistry, Wroclaw, Poland; 5 Technical University of Denmark, Department of Chemistry, Lyngby, Denmark; 6 Weizmann Institute, Department of Plant Science, Rehovot, Israel; 7 University of Bonn, IZBM, Bonn, Germany; 8 University of Fribourg, Adolphe Merkle Institute, Fribourg, Switzerland; 9 INRA-Research Unit Biopolymers Interactions Assemblies, Nantes, France; 10 The City College of New York, Institute for Macromolecular Assemblies New York, USA.

Despite a lot of progress in the elucidation of the cutin biosynthetic pathway, little

is known about the molecular mechanisms how cutin precursors are transferred

from the inside of the cell to the location of polymerization in the cuticle.

Following in vitro and in vivo approaches, we elucidate the role of the ABCG32

transporter in the formation of the cutin matrix.


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Elucidating the Role of BODYGUARD in Cuticle Formation Using Tomato (Solanum lycopersicum) Fruit as a Model

Glenn Philippe a, Trevor H. Yeats a, Mayank Kaashyap a, David S. Domozych b and

Jocelyn K.C. Rose a aPlant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853; b

Department of Biology, Skidmore College, Saratoga Springs, New York 12866

BODYGUARD is a protein implicated in establishing cuticle architecture, however its

specific enzymatic activity and mode of action remain unknown. To investigate its

function, we have focused our research on the tomato homolog SLBDG2 and

characterized the effects of the gene knock out on fruit cuticle composition, organization

and properties.

SESSION 2

Structure and properties of cutinized,

suberized and lignified cell walls

Structure and properties of cutinized, suberized and lignified cell walls PADiBa 2019

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The role of the plant cuticle during apical hook development Sara Raggia, Sijia Liua, Stéphanie Roberta a Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden

The apical hook is a transient structure established by dicotyledonous plants in order to

protect their meristematic cells in the early stage of their development right after

germination. In our study we identified a link between an extracellular polyester layer

named cutin and phytohormones in the regulation of apical hook development.


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The Role of Suberized Exodermis in Plant Nutrition and Growth Dolors Company-Arumía, Mercè Figuerasa, Mònica Iglesiasb, Eva Marguíb, Katarina

Vogel-Mikusc, Enriqueta Anticób and Olga Serraa a Laboratori del Suro, Departament de Biologia, Universitat de Girona, 17003 Girona, SPAIN; b Departament de Química, Universitat de Girona, 17003 Girona, SPAIN; c Department of Biology, University of Ljubljana, Ljubljana, SLOVENIA

Little is known about the function of suberin exodermis in plant nutrition. Potato plants

grown in hydroponics presented the root suberin mostly restricted to the exodermis. We

used these conditions to grow wild-type and a suberin-deficient mutant plants to study

the consequences of the reduced exodermal suberin in metal ion distribution and plant

growth.


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Root Cap Cuticle: Structure and Functions Alice Berhina, Damien de Bellisa, Rochus B. Frankeb, Rafael A. Buonoc,d,

Moritz K. Nowackc,d and Christiane Nawratha a University of Lausanne, Lausanne, Switzerland b University of Bonn, Bonn, Germany c Ghent University, Ghent, Belgium d VIB Center for Plant Systems Biology, Ghent, Belgium

Alice Berhin

The root cap is thought to protect the root meristem and plays an important role in

sensing the environment. Already formed during embryogenesis it might play a role

during the establishment of the seedling after germination. An intriguing expression of

genes required for cutin biosynthesis in the outermost root cap cells lead to the

discovery of a root cap cuticle in young Arabidopsis seedlings.


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Cross-talk between the cutin polyester and cell wall polysaccharides Bénédicte Bakana, G. Philippea, N. Geneixa, J. Petitb, F. Guillona, C. Sandtc, C. Rothanb,

M. Lahayea, D. Mariona. a Unité Biopolymères, Interactions, Assemblages, INRA, BP71627 44316 Nantes Cedex 3 b UMR 1332 BFP, INRA, Univ. Bordeaux, 33140 Villenave d’Ornon, France. c Synchrotron SOLEIL, Ligne SMIS, L'Orme des Merisiers, 91192 Gif-sur-Yvette, France.

Structural characterization of cutinized polysaccharides either from WT tomato fruits or

from mutants affected in cutin polymerization highlights a cross-talk between the cutin

polyester and cell wall polysaccharides. New models for the formation of these complex

lipopolysaccharide edifices are proposed.


38

Aromatic Compounds in Cuticular Waxes: Biosynthesis, Localization, and Potential Function

Yulin SUNa, Alvaro LUNA-CORTESa, Reinhard JETTERa,b a Department of Botany, University of British Columbia, BC; b Department of Chemistry, University of British Columbia, BC

The waxy cuticle coats epidermis cells to protect plants against stress. Significant

amounts of aromatic compounds are present in the cuticular wax of many species, and

surface tissues of cereal species are known to accumulate alkyl-substituted resorcinols

in particular. Here, we identified alkylresorcinol synthases from select grass species and

tested their involvement in cuticle formation.

SESSION 3

Regulation and development of cutinized,

suberized and lignified cell walls

Regulation and development of cutinized, suberized and lignified cell walls PADiBa 2019

40

Grain to Hull Adhesion in Barley Chiara Campolia, Linsan Liua, Trisha McAllistera, Micha Bayerb, Luke Ramsayb, Robbie

Waugha,b and Sarah M. McKim a aDivision of Plant Sciences, University of Dundee at; bThe James Hutton Institute, Dundee, UK

In barley, the grain pericarp secretes a cementing layer which firmly adheres to the hull.

We study mutants showing partial hull shed, or grain ‘skinning’, to reveal the genetic,

chemical and developmental mechanisms underlying variation in grain to hull adhesion

in barley.


41

Suberin plasticity and function for nutrient transport Marie Barberona a BIVEG, Science III, University of Geneva, Geneva, Switzerland

Suberization responds to a wide range of nutritional stresses. We are investigating the

gene regulatory network controlling suberization and are developing screens to identify

regulators of suberin deposition and plasticity in roots that will uncover suberin function

in plant adaptation.


42

Identification of Regulators for Increased Suberin Production Joanne Chorya,b,c, Julie Lawa,b, Joe Noela,c,d, Wolfgang Buscha,b aSalk Institute for Biological Studies, Harnessing Plants Initiative, La Jolla, USA bSalk Institute for Biological Studies, Plant Molecular and Cellular Biology Laboratory, La Jolla, USA cJack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, USA dHoward Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, USA

We are identifying pathways by which suberin can be increased in roots. In particular,

we are conducting quantitative, high-throughput phenotyping of roots for altered

periderm development and suberin content, making use of automated microscopy and

deep learning approaches to identify genes and their variants that are involved in

periderm formation and suberin deposition and assembly.


43

Modulating suberin deposition in the periderm: a MYB story Episode I David Molinaa, Rebecca Richtera, Anna Wunderlinga, Tonni G. Andersenbc and

Laura Ragnia a ZMBP- Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany; bMax Planck Institute for Plant Breeding Research, Cologne, Germany; cUniversity of Lausanne, Lausanne, Switzerland.

During secondary growth, the periderm is the outer tissue that covers and protects the vasculature. The periderm is formed by the phellogen a bifacial meristem, which produces inwards the phelloderm and outwards the cork. Our work highlights the importance of a set of MYB transcription factors during cork differentiation.


44

SUBERMAN is a component of the regulatory network establishing endodermis suberization of the Arabidopsis root

Hagai Cohena, Vadim Fedyuka, Chunhua Wangb, Shuang Wub, Asaph Aharonia a Department of Plant and Environmental Science, Weizmann Institute of Science, Rehovot, Israel; b FAFU-UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou China

The regulatory mechanism underlying root endodermis suberization is still elusive.

Here, we discovered and characterized the Arabidopsis SUBERMAN transcription factor

as a key mediator of suberin deposition in the root endodermis.

SESSION 4

Student Presentations

Student presentations PADiBa 2019

46

Modulating suberin deposition in the periderm: a MYB story Episode II

David Molina-Garcésa, Rebecca Richtera, Anna Wunderlinga, Tonni G. Andersenbc and


The periderm is the outer protective layer in secondary growth. It acts as a natural

barrier against pathogens, controlling at the same time gas exchange and water

equilibrium. Despite its importance, how the phellem differentiates and maturates is still

largely unknown. We will exploit the tools and the resources available in Arabidopsis to

elucidate the phellem transcriptional network.


47

Genetic and histological studies reveal a close association between flavonoids and an endosperm cuticle in Arabidopsis seeds

Lara Demonsaisa, Anne Utz-Pugina, Lena Hyvärinena, Sylvain Loubérya and

Luis Lopez-Molinaa a University of Geneva (UNIGE), Geneva, Switzerland

The seed is composed of an embryo surrounded by endosperm and the testa. Recently,

we characterized an endosperm-associated cuticle in mature seeds that contributes to

the seed’s biophysical properties. In this project, we seek to get insight into the

structural and biochemical organization and biophysical properties of this apoplastic

barrier and the role of flavonoids therein.


48

Two kinases regulated by MYB36 are essential for correct localization of lignin in Casparian strip domain

Qi Wu, Toru Fujiwara, Takehiro Kamiya Graduate School of Agricultural and Life Sciences, The University of Tokyo,Tokyo, Japan

Casparian strip, one of the cell wall modification made of lignin,functionsas a root

apoplastic barrier. Our study focuses on the underlying molecular mechanismsoflignin

depositionat thecorrect site.

SESSION 5

Mechanism and molecular factors

underlying apoplastic barrier formation

Mechanism and molecular factors underlying apoplastic barrier formation PADiBa 2019

50

Candidate genes involved in cork cambium activity and phellem differentiation highlighted by transcriptomic analysis

Susana T. Lopesa,b,c, Daniel Sobrald, Bruno Costaa,b,c, Pedro Perdigueroa,b,

Inês Chavesa,b,c, Augusta Costae, Célia M. Miguela,b,c a Instituto de Biologia Experimental e Tecnológica (iBET), Oeiras, Portugal b Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal c Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa (FCUL), Lisboa, Portugal d Instituto Gulbenkian de Ciência (IGC), Oeiras, Portugal e Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Oeiras, Portugal

Transcriptomic analysis of the secondary growth tissues cork cambium/phellem versus

vascular cambium/xylem highlighted candidate genes specifically involved in the cork

formation process. Apart from candidate regulators of suberin biosynthesis and

deposition, several transcription factors were identified that are putatively associated to

meristematic activity of the cork cambium.


51

Suberin dynamics during lateral root formation Robertas Ursachea, Cristovao Vieirab, Tonni Grube Andersena, Joop Vermeerb,

Niko Geldnera a Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland; b Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland

Plants roots take up nutrients and block out unwanted compounds from the soil by using

a selective barrier known as the endodermis. The endodermal suberin-based barrier

must be remodeled to allow lateral roots to emerge. Using various genetic tools, we

have identified several auxin-induced and endodermis-enriched candidate proteins

important for suberin remodeling during lateral root formation.


52

Walking a Waxy Path: Molecular Characterisation of Barley Eceriferum-yy

Chiara Campolia, Miriam Schreiberb, Zenith Tandukarc, Gary Muehlbauerc,

Robbie Waugha,b a Division of Plant Sciences at the James Hutton Institute, University of Dundee, Invergowrie, Dundee, UK; b Cell and Molecular Sciences Group, The James Hutton Institute, Invergowrie, Dundee, UK; c Department of Agronomy and Plant Genetics, University of Minnesota, St.Paul, MN, USA

The identification of genes regulating wax biosynthesis is a key step towards the

development of resilient varieties adapted to future agricultural scenarios. I will describe

my characterisation of barley mutant Cer-yy, impaired in wax biosynthesis. I used

chemical, genetic and transcriptomic approaches to support identifying Cer-yy and

understanding the role of this widely-distributed mutation.


53

Elucidating Exodermis: Suberization Kaisa Kajalaa, Alex Cantó Pastorb, Niba Nirmalb, Joel Rodriguez-Medinab, Concepción

Manzano Fernándezb, Lidor Shaar-Mosheb, Alex Masonb, Mariana A. Silva Artura,

Sharon Grayb, Siobhan Bradyb

a Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, Utrecht, The Netherlands b Plant Biology and Genome Center, University of California Davis, USA

Tomato exodermis is a suberized root cell layer that protects the root against drought

and salinity. We have generated tomato root cell type translatome and identified the

exodermal suberin biosynthesis pathway and its regulators.

SESSION 6

Diffusion barriers in biotic and abiotic

plant-environment interactions

Diffusion barriers in biotic and abiotic plant-environment interactions PADiBa 2019

55

Cuticular wax esters contribute to drought tolerance in Arabidopsis thaliana

Payal Patwari1, Peter Dörmann1* 1Institute for Molecular Physiology and Biotechnology of Plants, University of Bonn, Germany. *Corresponding author(s): [email protected]

Plants are covered with a microcrystalline layer containing cuticular waxes which forms

a protective barrier against water loss and other environmental stresses. Cuticular wax

is a complex mixture of very long chain fatty acids (VLCFAs) and derivatives such as

alkanes, alcohols, aldehydes, ketones and wax esters. The role of individual wax

components under drought stress is largely unknown.


56

Root apoplastic barriers contribute to defence against plant-parasitic cyst and root-knot nematodes

Julia Holbein1, Rochus B. Franke2, Peter Marhavy3, Satoshi Fujita3, Mirosława

Górecka4, Miroslaw Sobczak4, Niko Geldner3, Lukas Schreiber2,

Florian M.W. Grundler1, Shahid Siddique1,5 1Rheinische Friedrich-Wilhelms-University of Bonn, INRES – Molecular Phytomedicine, Karlrobert-Kreiten-Straße 13, D-53115 Bonn, Germany 2Rheinische Friedrich-Wilhelms-University of Bonn, IZMB – Ecophysiology, Kirschallee 1, D-53115 Bonn, Germany 3University of Lausanne, Department of Plant Molecular Biology, 1015 Lausanne, Switzerland 4Department of Botany, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-787 Warsaw, Poland 5Department of Entomology and Nematology, University of California, Davis, One Shields Ave., Davis, CA 95616, USA

Plant-parasitic nematodes induce specialized long-term feeding structures in root

vasculature from which they obtain their nutrients. By using microscopic, genetic, and

biochemical assays, we showed that the apoplastic barriers plays a dual role in

nematode parasitism, a barrier to nematode entrance and a regulator of nutrient

acquisition for nematodes from their feeding sites in roots.


57

Indoors and Open Air Analysis of a Dozen Genome Edited Tree Tobacco Lines Unveils the Significance of Cuticular Lipids in

Physiology and Fitness Boaz Negina, Shelly Hen-Avivia and Asaph Aharonia aDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

Despite decades of extensive research, the role of cuticular lipids in sustaining plant

fitness is not clear cut. To examine this topic, we created a tree tobacco (Nicotiana

glauca) population mutated in 17 different cuticular lipid related genes using the

CRISPR Cas9 system, and studied how alterations in their load and composition

affected both abiotic and plant – herbivore interactions


58

Posters

Poster - Biosynthesis, assembly and deposition of biomacromolecules in dermal tissuesPADiBa 2019

POSTER – 01

Modulating suberin deposition in the periderm: a MYB story Episode II

David Molina-Garcésa, Rebecca Richtera, Anna Wunderlinga, Tonni G. Andersenbc and


The periderm is the outer protective layer in secondary growth. It acts as a natural

barrier against pathogens, controlling at the same time gas exchange and water

equilibrium. Despite its importance, how the phellem differentiates and maturates is still

largely unknown. We will exploit the tools and the resources available in Arabidopsis to

elucidate the phellem transcriptional network.


POSTER – 02

Characterizing the developmental and water deficit-induced suberization of grapevine fine roots

A Li Zhanga, Pierre-François Berta, Gregory A. Gambettaa and Frédéric Domergueb a EGFV, Bordeaux-Sciences Agro, INRA, Univ. Bordeaux, ISVV, 33882 Villenave d'Ornon, France; b,LBM, CNRS, Univ. Bordeaux, UMR5200, 33882 Villenave d'Ornon, France.

This study provides a detailed characterization of the developmental and water deficit

induced suberization of grapevine fine roots and identifies important orthologous genes.


62

POSTER – 03

Characterization of ABCG32 homologues in tomato Carolina Elejaldea, Aurore Gueraulta, Damien de Bellisb, Antonio Mucciolob,

Bénédict Bakanc and Christiane Nawratha a University of Lausanne, DBMV, Lausanne, Switzerland; b University of Lausanne, EMF, Lausanne, Switzerland; c INRA-Research Unit Biopolymers Interactions Assemblies, Nantes, France

During the last decade many aspects of the cutin biosynthetic pathway have been

elucidated. However, questions about the transfer of cutin precursors outside of the cell

still need to be addressed. By using the tomato cv MicroTom plant model and the

CRISPR Cas9 approach we plan on finding some keys to open the black box

surrounding this particular step.


POSTER – 04

Involvement of Arabidopsis ELT-Enzymes in Wax Ester Biosynthesis Nina Hoppea, Regina Wehlera, Peter Dörmanna a Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53119 Bonn, Germany

Fatty acid phytyl esters in Arabidopsis are synthesized by PES1 and PES2, enzymes of

the ELT acyltransferase family. We studied the substrate specificities and localization of

the phytyl esters after expression in Nicotiana benthamiana.


64

POSTER – 05

Tomato mutants for deciphering fruit cutin biosynthesis and regulation

Petit Ja, Bres Ca, Mauxion J-Pa, Domergue Fb, Rose JKc, Rothan Ca a INRA and University of Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, INRA, 33140 Villenave d’Ornon, France ; b Laboratoire de Biogénèse Membranaire, Université de Bordeaux, UMR5200, F-33000 Bordeaux, France ; c Department of Plant Biology, Cornell University, Ithaca, NY, 14853, USA

Combining phenotypic diversity available in our tomato EMS mutant collection with mapping-by-sequencing strategy allowed us to identify several mutants involved in overall cutin formation. This set of cutin-deficient mutants disrupted at different steps provided an excellent resource for investigating cutin formation.


POSTER – 06

Broad Implication of Glycerol-3-Phosphate Acyltransferases in Endodermal Suberin Formation

Alice Berhina, Nasim Farahani Zayasa, Rochus B. Frankeb, Christiane Nawratha

a Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland; b Institute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany

The glycerol-3-phosphate acyltransferase (GPAT) family is involved in polyester

formation. The implication of the GPAT5/GPAT7 as well as the GPAT4/GPAT6/GPAT8

branch of GPATs in endodermal suberin formation will be presented.


66

POSTER – 07

Investigations in the Cuticular Wax Esters of A. thaliana Flower Organs

Yannic Müllera, Veronika Salewskia, Lukas Schreiberb, Peter Dörmanna a Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Germany b Institute of Cellular and Molecular Botany, University of Bonn, Germany

Wax esters are important components of the cuticular wax fraction. Acyltransferases of

the WS/DGAT family, in particular WSD1, was previously shown to be crucial for stem

wax ester synthesis in Arabidopsis. Here, we present our analyses of wax ester

accumulation on flower organs of different Arabidopsis wsd mutants.

Poster - Structure and properties of cutinized, suberized and lignified cell walls PADiBa 2019

POSTER – 08

Luminescence of isolated tomato fruit cuticles Ana González Moreno1, Eva Domínguez2 and Antonio Heredia1

1IHSM La Mayora, Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, E-29071 Málaga, Spain 2IHSM La Mayora, Departamento de Mejora Genética y Biotecnología, Consejo Superior de Investigaciones Científicas, E-29750 Algarrobo-Costa, Málaga, Spain

This work is based on the study of the luminiscence properties of isolated tomato fruit

cuticles. The green fluorescence that takes place in the ripe stage of development.after

blue excitation is explained by the presence of naringenin chalcone. It could affect water

and chemical transport and mechanical behaviour of the tomato fruit cuticle.


68

POSTER – 09

Genetic and histological studies reveal a close association between flavonoids and an endosperm cuticle in Arabidopsis seeds

Lara Demonsaisa, Anne Utz-Pugina, Lena Hyvärinena, Sylvain Loubérya and

Luis Lopez-Molinaa a University of Geneva (UNIGE), Geneva, Switzerland

The seed is composed of an embryo surrounded by endosperm and the testa. Recently,

we characterized an endosperm-associated cuticle in mature seeds that contributes to

the seed’s biophysical properties. In this project, we seek to get insight into the

structural and biochemical organization and biophysical properties of this apoplastic

barrier and the role of flavonoids therein.


POSTER – 10

How to Affix a Lipid in the Endodermal Cell Wall? A Model Involves Peroxidase Catalyzed Cross-Linking

Mathias Brands, Christopher Hidalgo-Shrestha, Katharina Markus, Lukas Schreiber,

Rochus Benni Franke Institute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany

We investigated the contribution of individual aromatic monomers to the formation and

structure of Casparian strips and suberin lamellae in the endodermis. Here we present a

peroxidase that is involved in the cross-linking of phenylpropanoids in suberin.

Corresponding mutants show a disturbed suberin ultrastructure and a delay in the onset

of continuous endodermal suberisation.

Poster - Regulation and development of cutinized, suberized and lignified cell wallsPADiBa 2019

POSTER – 11

Outer bark transcriptome of cork oak, holm oak and their natural hybrids

Iker Armendariza, Unai López de Herediab, Álvaro Sotob, Olga Serraa, Mercè Figuerasa a Laboratori del Suro, Universitat de Girona, Campus Montilivi, 17003, Girona, Spain; b Sistemas Naturales e Historia Forestal, Universidad Politécnica de Madrid, 28040, Madrid, Spain

Cork oak and holm oak are closely related species that produces a very different outer

bark: the cork and rythidome, respectively. Interestingly, their natural hybrids produce

outer bark in between cork and rhytidome. Transcriptomic analysis of the outer barks

pretends to deep on mechanisms and genes controlling the development of these types

of outer bark.


POSTER – 12

Development and function of the hypocotyl apoplastic barriers in Arabidopsis

Inês CR Barbosaa and Niko Geldnera a Department de Biologie Molecular Vegetal, Université de Lausanne, Switzerland

The root endodermal cell wall is impregnated by two polymers that prevent apoplastic

leakage: localized lignin at the Casparian Strip (CS), and suberin, as suberin lamellae.

The hypocotyl connects root and aerial organs in young seedlings. Its endodermis bears

a CS, and epidermis deposits cutin. We investigate the development of the hypocotyl

CS and its function for seedling establishment.


72

POSTER – 13

Understanding the Molecular Regulation of Periderm Suberization in Root Secondary Growth

Ana R. Leal a,b, P.M. Barrosa, B. Parizotb, J. Beloa, N. Vangheluweb, T Beeckmanb &

M.M. Oliveiraa a Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA) & iBET, GPlantS, Av. da República, 2780-157 Oeiras, Portugal; b Ghent University, Department of Plant Biotechnology and Bioinformatics, and VIB Center for Plant Systems Biology, (Technologiepark 71) 9052 Ghent, Belgium

Development and suberization of periderm cells are a tightly controlled processes,

however their regulation is still poorly characterized. In this work, we performed a

histological study and specific transcriptomic analyses targeting periderm development

in roots from Arabidopsis and cork oak. Integration of results from both species reveals

conserved mechanisms of this development process.


POSTER – 14

Integrative analysis of two closely-related russeted and non-russeted apple varieties during fruit development provides new insights on

triterpene composition and the cuticle/suberin interplay. Sylvain Legaya, Gea Guerrieroa, Jean-Francois Hausmana, Céline Leclerqa, Kjell

Sergeanta, Jenny Renauta, Christelle Andréa. a Luxembourg Institute of Science and Technology, Esch sur Alzette, Luxembourg)

Russeting, due to the deposition of suberin in the inner part of epidermal cell walls, is a

major concern for the apple fruit production leading to an important price devaluation for

a part of the production. In the present work, the fruit growth development of two closely

related russeted and non russeted varieties was investigated combining transcriptomics,

proteomics and metabolomics.


74

POSTER – 15

PttHB6, HD-Zip III gene, has novel functions in regulation of wood chemistry in Populus

Ana Milhinhosa,#, Lorenz Gerber b, Andras Gorzasb,c, Leonardo Gomez d, Gaia Geiss b,

Simon McQueen-Masone, Magnus Hertzberg d, Björn Sundbergd, Hannele Tuominen a a Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden; b Swedish University of Agricultural Sciences, Umeå;, Sweden, c Dept of Chemistry, Umeå University, Umeå, Sweden, d SweTree Technologies, Umeå, Sweden, e University of York, York, United Kingdom, # present address: ITQB-NOVA, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal, contact e-mail: [email protected].

Populus Class III Homeodomain-Leucine Zipper (HD-Zip III) transcription factor

CORONA/AtHB15 (CNA) is implicated in plant vascular development, yet, knowledge of

their role in wood formation is still very scarce. We present work demonstrating a novel

role for HD-Zip III genes in regulation of wood chemistry in Populus.


POSTER – 16

Understanding the Significance of Adaptive Suberin Plasticity Jian-Pu Han, Vinay Shukla, Marie Barberon Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland

Endodermal suberin lamella, a highly plastic barrier, fine tuning radial transport of

nutrients. We are developing a high-throughput screening of 250 natural variations of

Arabidopsis in order to decipher suberin function in plant adaptation. Combined with

genome-wide association studies these analyses will gain insight into the specific

molecular factors driving adaptive and plastic suberization.


76

POSTER – 17

Characterization of unsuberized passage-cells at the exodermis in annual wild species of Echinochloa

Katsuhiro Shionoa, Masato Ejiria a Graduate School of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji, Japan

Not only endodermis it is also known passage cells in the outer part of roots, which lack

suberin lamellae and lignin deposits, are found in a wetland plant. However, it is

unknown that development of passage cells at the exodermis. Here, we characterize a

development of passage cells in an annual wild species of Echinochloa.


POSTER – 18

Drought Regulation of Tomato Root Exodermis Suberization Mariana A. Silva Artura, Niba Nirmalb, Concepción Manzano Fernándezb, Lidor Shaar-

Mosheb, Alex Cantó Pastorb, Siobhan Bradyb, Kaisa Kajalaa a Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, Utrecht, The Netherlands b Plant Biology and Genome Center, University of California Davis, USA

The exodermis is a specialized root cell layer responsive to drought. Suberization is one

of the major characteristics of exodermis, however, gene regulatory network controlling

suberization is still unknown. We have identified transcription factors associated with

exodermis differentiation and suberin biosynthesis in tomato, and we are testing their

role under osmotic stresses in hairy roots.


78

POSTER – 19

An apoplastic barrier-mediated tissue separation in anthers allows correct pollen development through small peptide-receptor like

kinase interactions Jekaterina Truskinaa, Satoshi Fujitab, Nicolas Dolla, Niko Geldnerb, Gwyneth Ingrama a Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France; b Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland

Correct pollen development in anthers requires communication between the maternal

sporophytic tissues and the developing gametophytic pollen. A new apoplastic barrier in

anthers separates the sporophytic tissues from the zone of active pollen development.

The interaction between these zones is achieved through small mobile peptides and the

Leucine Rich Repeat receptor-like kinases.


POSTER – 20

Some shall pass: Suberin plasticity for nutrient acquisition Vinay Shuklaa, Linnka Legendre Lefebvrea, Marie Barberona aDepartment of Botany and Plant Biology, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland

Suberin coating of endodermal cells controls nutrients uptake and is regulated by

nutrient availability. However, the signaling cascade involved remains largely unknown.

Our study introduces novel regulators of nutrient barrier controlling the root suberization.

Our work also demonstrates the physiological relevance of the ectopic suberization in

response to nutrient stress.

Poster - Mechanism and molecular factors underlying apoplastic barrier formation PADiBa 2019

POSTER – 21

Understanding the Role of Barley Mutant cer-g During Grain to Hull Adhesion

Linsan Liua, Chiara Campolia, Micha Bayerb, Robbie Waugha,b and Sarah M. McKima aDivision of Plant Sciences, University of Dundee at: bThe James Hutton Institute, Dundee, Scotland

In barley, the hull normally firmly sticks to the grain. Partial hull shed or ‘skinning’ of the

grain is an undesirable trait reflecting a compromised quality of grain hull adhesion.

Here, I use a wax-deficient barley mutant showing skinning to investigate the underlying

structural, chemical and genetic basis of grain to hull adhesion in barley.


POSTER – 22

Get a Grip – Mechanisms Underlying Variation in Barley Grain Hull Adhesion

Trisha McAllistera, Chiara Campolia, Robbie Waugha,b, Sarah M. McKima a Division of Plant Sciences, University of Dundee at: b The James Hutton Institute, Dundee, Scotland

Barley grain is unique among cereals in being enclosed by an adherent hull. We know

that hull adhesion occurs via production of an adhesive layer which is deposited onto

and may modify the grain cuticle. I aim to further elucidate the mechanisms behind hull

adhesion by studying grain skinning – a grain quality defect which results in partial hull

shed in barley.


82

POSTER – 23

How cork cells differentiate: the role of Programmed Cell Death and proanthocyanidin deposition in cork oak periderms

Vera Inácioa, Carolina Lobatoa, José Graçab, Leonor Morais-Cecílioa a Linking Landscape, Environment, Agriculture and Food - LEAF, Institute of Agronomy, Lisbon, Portugal; b Forest Research Center, Institute of Agronomy, Lisbon, Portugal

Vascular plants with secondary growth cope with environmental hostile conditions by

developing periderms mostly composed of dead suberized cells. We use cork oak as a

model to study its exceptional periderm formed by a long-lived phellogen. With this

work, we intend to disclose the periderm ontogenic program by studying the

differentiation steps of original and traumatic phellogen derived cells.


POSTER – 24

Role of the HAESA signalling pathway and LACS in cell separation and sealing in the abscission process in Arabidopsis Thaliana.

Sebastian Augustin, Julia Santiago Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland

The receptor HAESA and the peptide IDA regulate abscission in Arabidopsis. We want

to dissect in mechanistic terms how activation of this membrane signaling pathway is

translated into structural changes of the cell wall.


84

POSTER – 25

Two kinases regulated by MYB36 are essential for correct localization of lignin in Casparian strip domain

Qi Wu, Toru Fujiwara, Takehiro Kamiya Graduate School of Agricultural and Life Sciences, The University of Tokyo,Tokyo, Japan

Casparian strip, one of the cell wall modification made of lignin,functionsas a root

apoplastic barrier. Our study focuses on the underlying molecular mechanismsoflignin

depositionat thecorrect site.

Poster - Diffusion barriers in biotic and abiotic plant-environment interactions PADiBa 2019

POSTER – 26

Exodermis differentiation under nutrient deficiency Jiří Namyslova, Zuzana Blascheováa, Jana Janouškováa, Aleš Soukupa, Edita Tylováa a Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic

Exodermis differentiation under nutrient deficiency. Exodermis is the non-obligatory root

apoplastic barrier that functions as the protective layer. In our anatomical study, we

focused on the relationship between exodermis maturation and nutrient availability

using maize and barley as the model species. Key-words: Exodermis, nutrient

availability, maize


86

POSTER – 27

Cuticular wax coverage and its transpiration barrier properties in Quercus coccifera L. leaves: does the environment matter?

Amauri Buenoa, Domingo Sancho-Knapikb, Eustaquio Gil-Pelegrínb, Jana Leidea,

José Javier Peguero-Pinab, Markus Burghardta, and Markus Riederera a University of Würzburg, Julius von Sachs Institute of Biological Sciences, Chair of Botany II – Ecophysiology and Vegetation Ecology, Julius-von-Sachs-Platz 3, 97082 Würzburg, Germany; b Unidad de Recursos Forestales, Centro de Investigación y Tecnología Agroalimentaria de Aragón, Gobierno de Aragón, Avda. Montañana 930, 50059 Zaragoza, Spain.

Drought-stressed plants close stomata to reduce water loss and, therefore, the

remaining transpiration only occurs through the cuticle. During heatwaves, excessive

cuticular water loss may lead to sudden xylem cavitation. Thus, efficient control of

cuticular water loss is of fundamental importance for maintaining xylem hydraulic safety,

thereby ensuring plant reproductive fitness and survival.


POSTER – 28

Ecophysiology with barley eceriferum (cer) mutants

Penny von Wettstein-Knowles Biology Department, Copenhagen University, Ole Maaloees Vej 5, DK-2200 Copenhagen N, Denmark

In addition to preventing water loss, plant cuticles must also regulate nutrient loss,

leaching. Pertinent barley mutants were characterized for water loss rate, reaction to

toluidine blue and simulated rain delivered during vegetative growth or the maturation

phase. Appropriate controls were included. Nutrients and water going through the soil

as well as nine phenotypic traits were measured.


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POSTER – 29

Cuticular barrier of wild and cultivated Solanum species Aline X. de Souza, Jana Leide and Markus Riederer Chair of Botany II – Ecophysiology and Vegetation Ecology, Julius-von-Sachs-Institute for Biosciences, University of Würzburg, Würzburg, Germany

This study compares the efficiency of the cuticular transpiration barrier among different

Solanum species. Cuticular function and chemistry were assessed for fruits and leaves

of fourteen selected plant species. We investigate intra- and interspecific cuticular

properties among cultivated plant species in relation to their wild relatives.


POSTER – 30

The impact of endodermal diffusion barriers on root to shoot transport of potassium in A.thaliana

Andreas Kolbeck Université de Lausanne, Lausanne, Switzerland

Plant roots rely on formation of diffusion barriers to achieve selective uptake of solutes

and to efficiently transport them to the shoot. Ionomic profiles of shoot tissues from

mutants in Casparian Strip development show significant differences for the highly

mobile macro-nutrient potassium. Our work tries to elucidate direct and indirect impacts

of barrier formation on potassium transport.


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POSTER – 31

Genomic, chemical and functional analysis of adult leaf cuticle development in maize

Richard Bourgaulta, Pengfei Qiaob, Susanne Matschic, Miguel Vasquezc, Annika

Sonntaga, Caleb Charleboisa, Marc Mohammadia, Michael Goreb, Laurie G. Smithc,

Michael J. Scanlonb, and Isabel Molinaa, aDepartment of Biology, Algoma University, Sault Ste. Marie, ON P6A 2G4, Canada; bPlant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA; cSection of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA

The plant cuticle establishes a vital interaction interface with the environment. The

functional contributions of the maize cuticle and its components to its barrier property in

the adult plant, agronomically the most important growth phase, are unknown. We

utilized the expanding adult leaf as a model system to characterize the cuticle

maturation along the leaf developmental gradient.

Sponsor We greatly appreciate financial support by the:

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