4_Razzaque

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Curriculum Vitae

Provide a brief CV showing education and research training, including any prizes or awards: (no

more than one page)

Education:

MSc in Biotechnology; CGPA 3.93 out of 4; Academic Standing: Highest Distinction

Research Experiences:

1. MS Fellow, Bangladesh Academy of Sciences - United States Department of

Agricultural funded project for salinity tolerant tomato production employing transgenic

approach.

Accomplishment: MS thesis entitled “Cloning of an antiporter gene from PCR

amplicons into a recombination competent vector containing a constitutive prompter

(CaMV35S)”

Duration: November 2010 – October 2011

2. Research Associate, Plant Biotechnology Lab, Department of MNS, BRAC University,

Dhaka, Bangladesh

Responsibilities: Biological data analysis applying novel and existed bioinformatics

tools; Agrobacterium mediated Transformation, Cloning; Lab management, Presentation

on the work progress, Guide on the other MS students’ MS thesis work, Lab

procurements etc.

Duration: November 2011 to till to date

Training and Conferences:

1. 5th

International Conference on Microbiology of Food, Health and Environment:

Problems and Prospect in Developing Countries, Organized by Microbiology

Department, University of Dhaka.

2. Five day long workshop in Immunology and High Throughput Techniques at

Immunology Lab, ICDDR,B, Dhaka

3. Training Program in General Laboratory Safety, Safe Laboratory Design, Chemical

Safety, Waste Management and Bio-safety, ICDDR,B, Dhaka

4. Conference of the Bangladesh Society for Biochemistry and Molecular Biology, 2012

Organized by: Bangladesh Society for Biochemistry and Molecular Biology

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5. 3rd

National Congress on Biotechnology and its prospect, Organized by Young

Biotechnologists Bangladesh.

6. Novartis Biocamp-2011

7. Participation as a surveyor in a base line survey on ‘Situation analysis of Medical

Biotechnology in Bangladesh’. Directed by Centre for Medical Biotechnology, Govt. of

Bangladesh.

Awards and Achievements:

1. Performance based tuition fees waiver at BRAC University during MS

2. Full tuition Fees Waiver at Higher Secondary Level from A.M.R. College

3. Board Stipend after Secondary School Certificate Examination

4. Junior Scholarship

5. Primary Scholarship

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List of Publications in International Journals*

Publications:

1. Amitav Das, Alak K Dutta, S. Razzaque, B.Saha, Partha S. Gope (2011), ‘Analgesic

and Antidiarrheal Properties of the Latex of Calotropis Procera’, International

Journal of Pharmaceutical & Biological Archives, Page 521-525

2. Md.Abu shoyeb, Samsad Razzaque, Aubhishek Zaman, Mohammed Rahamatulla

(2012), ‘Assaying Antihyperglycemic Effects of Crude Methanol Extract of

Amaranthus spinosus in Swiss Albino Mice’, International Journal of Research in

Phytochemistry and Pharmacology. Accepted

3. Abstract Publication on ‘Designing an in-silico protein-protein interaction profile

and gene regulatory network for Kaposi’s Sarcoma-associated Harpes Virus’ in an

ICGEB, New Delhi Symposium on ‘Anti-virals for the Developing Worlds’.

4. Samsad Razzaque, Naiyyum Choudhury (2012), ‘Techniques of Proteomics and its

Application in Health and Disease Control’, Bangladesh journal of Medical Sciences.

Submitted

5. Abstract Publication on ‘Proteomics in Health and Disease Control’, Published in 5th

International Conference on Microbiology of Food, Health and Environment: Problems

and Prospect in Developing Countries, Organized by the Department of Microbiology,

University of Dhaka.

6. Abstract publication on ‘Molecular cloning of the Na+/H+ exchanger gene from

Arabidopsis thaliana and Oryza sativa for tomato (Lycopersicon esculentum Miller)

transformation and conferring salt tolerance’ in Biochemistry and Molecular Biology

Conference, Rajshahi University, Bangladesh.

7. Manzur –E- Mohsina Ferdous, Samsad Razzaque, Debashis Chakrobarty, Zeba Islam

Seraj, Aparna Islam (2012), ‘Cloning of Na+

/H+ antiporter gene and establishment of

in vitro regeneration protocol in tomato (Lycopersicon esculentum Miller) to develop

salinity stress tolerant variety’, International Conference on Biotechnology and Food

Engineering. Accepted

8. Taslima Haque, Samsad Razzaque, Zeba Islam Seraj (2012) ‘In silico prediction of

MicroRNA and their targets in Plasmodium Falciperum’, Manuscript in Progress.

9. Samsad Razzaque, ‘Cloning of an Antiporter gene from PCR amplicons into a

recombination competent vector containing a constitutive promoter’. MS thesis.

BRAC University online library.

Popular Science article for GNOBB (www.gnobb.org)

1. Evidence Adduced for Lateral Gene transfer in Eukaryotes, published online at GNOBB

website on March 6, 2012

2. Cross kingdom transfer of functionally active miRNAs, published online at GNOBB

website on March 22nd

, 2012

3. Novel Mechanism of Influenza Virus against Human Antiviral Response, published

online at GNOBB website on March 31st , 2012

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4. The sequencing of tomato genome: Hopes and Prospects, published online at GNOBB

website on June 13 , 2012

5. Comparative Epigenomics - A classified approach to functionally annotate genomes,

published online at GNOBB website on June 17 , 2012

6. Influenza virus: Transmission possibility between mammals, published online at GNOBB

on June 27, 2012

7. Genome sequencing of bonobo: A comparative study on evolutionary relationship,

published online at GNOBB on July 1, 2012

8. A computational model proposed to elucidate mutation scenario, published online at

GNOBB on July 4, 2012

9. Impact of the mechanical pressure during cell division, published online at GNOBB on

July 8, 2012

10. Salt cress genome provides new clues for adaptive evolution, published online at


11. A Unique Computer Model Proposed for Mycoplasma genitalium, published online at


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12. Abstract

Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB

Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract)

Designing an In-silico protein–protein interaction profile and Gene regulatory network for

Kaposi’s sarcoma associated Herpes Virus

Aubhishek Zaman1, 4, Md. Habibur Rahaman1, 3, 4, Samsad Razzaque2, 3, 4

1. Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-

1000, Bangladesh

2. Biotechnology Program, Department of MNS, BRAC University, Dhaka-1212, Bangladesh

3. Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University

of Dhaka, Dhaka- 1000, Bangladesh

4. Contributed equally

ABSTRACT:

Herpes Simplex Virus 8 (HSV8) is the causative agent for a cancerous tumor of connective tissue which is

widely known as Kaposi's sarcoma. The cancer involves skin, lungs, gastrointestinal tract, and other organs

and is often associated with AIDS. Inspired by an urge to design an effective drug against the virus through a

systems approach, here we have tried to establish a regulatory network involving key host virus

interactions. The network comprises uncharacterized ORFs of the virus as well as interacting host proteins.

We collected sequence information from public databases and structurally characterized the protein

products of the ORFs using online servers- PSIPRED, CPHmodels- 3.0 and I-TASSER. For functional analysis

of the individual ORFs MotifFinder tool was used. Consequently partner profile for each ORFs was predicted

using String 9.0. Data mining tool Ali Baba further helped us to integrate predicted networks and

interactions. SAGE and Microarray data, signifying gene expression temporally, was useful to quantify

association between the nodes.To compare our interaction topology with standard known ones, PathBLAST

and NetworkBLAST were used. Moreover, MicroRNAs were predicted applying online tools and databases

like RNAFold, MatureBayes, Mipred etc. Targets of the predicted MicroRNAs were also analyzed applying

Miranda in the host and Human. Finally, with the help of Cytoskape and CentiBin bioinformatic platform the

results were represented as networks. Our study establishes both a protein-protein interaction profile and

a gene regulatory network. The topology of the protein-protein interaction profile helped us to predict a

pathway whereas the gene regulatory network revealed binary relationships among the genes. This study

needs to be further investigated and validated in vitro. The network we propose here can be used to design

antivirals in the future against HSV8 by targeting the network.

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Personal Statement

Indicate briefly here your Research Interests and Career Goals, why you are interested to

participate in the YSP Program (including what you will bring to the YSP and what you hope to

gain from it): (no more than one page)

Science has always been a passion to me since high school days. A basic and fundamental difference

with the glimpse of epigenomics, the whole structure of life is a mystery to me. I started finding the

answer through the available source, and then I decided to pursue my Undergrad in Zoology and then did

my MS in Biotechnology with a thesis in Molecular Biology. I am still on my way searching the answer

and the beauty of nature in creating such ways to transform the characters into a multi tasking approach

still amaze me.

To build up a greater or significant community in science it is very important to establish an interacting

environment to do the sharing and thoughts. I believe interacting with researchers around the globe will

surely enhance my ideas and thoughts towards science more precisely. This opportunity will apparently

help me in understanding the trends and challenges in the expansion of different applied and classical

biological sectors like Cell biology, Genetics, Human biology, Microbiology, and newly developed

biological techniques. This knowledge will not only allow me in better understanding biological and

natural principles but will also allow me to learn all the breakthrough of new discoveries and the trends

& challenges in the applied biology sector. This conference will also give a chance to interact with key

experts and leaders from all around the world. Therefore, I believe attending in this conference of

FAOBMB will provide the necessary background for me to be to anticipate and to react to ever-changing

environments using the experience of the experts.

In this regard I would like to mention that during my undergraduate and graduate studies, emphasis was

given entirely to understand the fundamental biological principles that govern modern day of Biological

advancements. I also learned about other topics such as bio-safety, laboratory management and

recombinant DNA technology. I am highly interested to deal with novel Biological techniques to follow

up the regulation and corresponding genetic pathways that lead to specific functional characteristics and

want to find out specific regulatory promoters and specific signaling pathways from both the indoor and

outdoor influences. My aim in life is to explore and to see the inner beauties of genetic mechanisms

through all possible ways. I am very much interested in Molecular Biology, Cell Biology, Drug

Designing, Neural Biochemistry and Bioinformatics. Being a part of the program, I will get a more

expand area on my thoughts and beliefs. I would really love to be a part of the event and waiting like to

see a horizon through it.

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Attachments:

• Letters of recommendation from two referees.

Submission methods: You can apply in either of two ways:

• by sending an email with scanned attachments to the Chair of the FAOBMB Fellowship

Committee, Prof. Piamsook Pongsawasdi:

[email protected] or [email protected]

This is the preferred method. If using this method, please assemble the Application Form and the

Attachments into a single PDF file.

• by sending a hard copy of the application form and supporting documents to:

Professor Piamsook Pongsawasdi,

Department of Biochemistry, Faculty of Science,

Chulalongkorn University,

Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7 Hours).

Applicants will be notified by email of the decision of the Committee by no later than 15 September,

2012.

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BRAC UNIVERSITY To whom it may concern

It is a great pleasure to spend a moment writing this letter of recommendation for Mr. Samsad

Razzaque whom I have known for more than 2 years as he has taken the following courses which

I teach:, Environmental Biotechnology, Fermentation and Industrial Biotechnology and

Enzymology.

I had the opportunity to work with Samsad in most of the faculty’s activities. He showed true

leadership characters when working with people, utilizing diplomatic approach. As a result of his

manners, his friends always supported him.

In class, Samsad showed his intelligence, creativeness, and “high responsibility” attitude. His

grade is the indicator of intelligent and academic integrity. As per his performance records, he

can be on the overall in the top of the class. He is intelligent, inquisitive and yearns to gain an in-

depth knowledge. With his determination and hard work I have no doubt that he will succeed in

all his endeavors.

With the combination of his leadership character, knowledge and intelligence, talent, and much

contribution to fellow students and faculty activities, he was one of my outstanding students.

I strongly recommend him with pride and confidence without any reservations and wish him

further success.

Sincerely,

Dr. Naiyyum Choudhury, Professor

Coordinator, Biotechnology Program

Department of Mathematics and Natural sciences

BRAC University

66 Mohakhali, Dhaka-1212

Bangladesh

Mobile No +88-01712-680-879

E-mail: [email protected]

Website: http://www.bas.org.bd/fellowship/list-of-fellows-/userprofile/naiyyum.html

66 Mohakhali, Dhaka-1212, Bangladesh Tel: +880 2 882 4051 Fax +880 2 8810383 e-mail: [email protected] www.bracuniversity.ac.bd

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BRAC UNIVERSITY

To Whom It May Concern

I know Mr. Samsad Razzaque since January 2010 in the capacity of his course teacher, guide for his

research programme and PI of the project he is working at the moment. His performance was outstanding

as a student and also as a researcher. Mr. Razzaque has kin aptitude for science specially in the field of

Molecular Biology and Bioinformatics and have an open mind to learn more and more. He actually

believes that there is no end of learning.

Mr. Razzaque possesses good general knowledge and analyzing capacity related to research issues with

productiveness. As his project supervisor, I had the occasion to evaluate his working attitude and trouble

shooting of any research hurdle which really amazed me. His presentation skill is equally appreciable.

This I witnessed when he offered a seminar on his project “Cloning of an Antiporter Gene from PCR

amplicons into a recombination competent vector containing a constitutive promoter”. His mode of

presentation using the audio visual aid was really impressive.

Mr. Razzaque has the aptitude, thorough knowledge and management skills in carrying out any given

assignment with perfection and within time. I found him excellent as group worker and his potential as a

group leader is really highly recommendable.

A candidate with good reading, writing and communication skills, I trust Mr. Razzaque will fulfill the

academic expectations of your esteemed faculty. I am sure that with the guidance and expertise of your

renowned graduate school, he will be able to develop independent school of thought and judgment

towards the life science research.

He has maintained a consistent academic record of CGPA 3.93 out of 4.00 and in my opinion I rank him

among the top 5% of the students I have come across. He is a candidate with reading and writing skills

and I am sure that he will fulfill the academic expectations of your University. His conduct and character

are very good and I very strongly recommend him for admission in your University. In one word I am

proud to be his teacher and guide. I wish him all success in his future academic feat.

Sincerely,

Aparna Islam, PhD

Editor, Journal of Plant Tissue Culture & Biotechnology (PTC&B)

Website of PTC&B: http://www.baptcb.org/

Assistant Professor, Biotechnology Program,

Department of Mathematics and Natural Sciences, BRAC University, Dhaka-1212, BD Ph: 00-

88-8824051-4, extn 4153

Ph: 00-88-02-8954487; 00-88-01817114304 (m), Skype:aparnai20

66 Mohakhali, Dhaka-1212, Bangladesh Tel: +880 2 882 4051 Fax +880 2 8810383 e-mail: [email protected] www.bracuniversity.ac.bd

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4_Razzaque