PATENT ABSTRACTS 299

4952707

E N Z Y M A T I C A L L Y - C L E A V A B L E C H E M I L U M I N E S C E N T F U S E D

POLYCYCLIC R I N G - C O N T A I N I N G 1 , 2 - D I O X E T A N E S

Brooks Edwards, Irena Y Bronstein, Alison A Laird, John Voyta assigned to Tropix Inc

Chemiluminescent 1,2-dioxetane compounds are disclosed in which the molecule is stabilized at the 3-position on the dioxetane ring against decomposition prior to the molecule's coming in contact with a labile group-removing substance (e.g., an enzyme that will cleave the labile group to cause the molecule to decompose to form at least one light-emitting fluorophore) and sub- stituted at the 4-position on the dioxetane ring with a fused polycyclic ring-containing fluorophore moiety bearing a labile ring sub- stituent whose point of attachment to the fused polycyclic ring, in relation to this ring's point(s) of attachment to the dioxetane ring, is such that the total number of ring atoms separating these points of attachment, including the ring atoms at the points of attachment, is an odd whole num- ber. These odd pattern substituted compounds decompose to emit light of greater intensity and of a different wavelength than that emitted by the corresponding even pattern substituted isomers. They are useful in detecting the pre- sence or determining the concentration of chem- ical or biological substances in immunoassays, chemical assays and nucleic acid probe assays, and in chemical/physical probe procedures for studying the microstructures of macro- molecules. Two or more of them can also be used in combination, or one or more of them can be used together with other chemiluminescent com- pounds, in multi-channel assays to detect two or more different analytes simultaneously. Novel intermediates used in the preparation of these odd pattern substituted compounds are also dis- closed.

the presence of other detergent components, espacially strong bleaching agents. The coating is usually carried out in a fluid bed. Also coatings with antioxidants and acid coatings enhance the enzymatic stability.

4973550

P R O C E S S F O R T H E E L E C T R O C H E M I C A L

R E G E N E R A T I O N O F C O - E N Z Y M E S

Frederick G Drakesmith, Clwyd, United King- dom assigned to The Electricity Council

A process for the electrochemical regeneration of a co-enzyme, which process comprises the steps of (i) electrochemically oxidizing a medium containing oxidized co-enzyme and bio- chemically inactive isomers of the co-enzyme to oxidize the biochemically inactive isomers, (ii) generating a mercury amalgam in an elec- trochemical cell; (iii) contacting in a reaction ves- sel the mercury amalgam and the medium containing the oxidize co-enzyme from step (i), with one of the reactants selected from the mer- cury amalgam and the oxidized co-enzyme being in the form of a plurality of droplets.

4975278

A N T I B O D Y - E N Z Y M E C O N J U G A T E S I N C O M B I N A T I O N

W I T H P R O D R U G S F O R T H E D E L I V E R Y O F C Y T O T O X I C A G E N T S T O T U M O R C E L L S

Peter D Senter, Mark G Saulnier, Joseph P Brown, David Kerr assigned to Bristol-Myers Company

4975453

4973417

E N T E R I C C O A T E D D E T E R G E N T E N Z Y M E S

Per Falholt, Gentofte, Denmark assigned to Novo Industri A/S

The granulate detergent enzyme product com- prises a core of a microbial enzyme containing material with an enteric coating. Due to the coating the enzymatic stability is enhanced, in

C O M B I N A T I O N O F A N G I O T E N S I N C O N V E R T I N G E N Z Y M E I N H I B I T O R S W I T H

P O T A S S I U M C H A N N E L M O D U L A T O R S A N D U S E

T H E R E O F I N P H A R M A C E U T I C A L S

Reinhard Becker, Rainer Henning, Hansjor Ur- bach, Volker Teetz, Heinrich C Englert, Dieter Mania, Wiesbaden, Federal Republic Of Ger- many assigned to Hoechst Aktiengesellschaft