PATENT ABSTRACTS 77

~ 8 M 1 2 4783524

H Y B R I D D N A S Y N T H E S I S O F E P I D E R M A L G R O W T H F A C T O R

G R O W T H F A C T O R S

Graeme I Bell assigned to Chiron Corporation

DNA sequences and methods of obtaining DNA sequences which include a sequence encoding for mammalian epidermal growth fac- tor are provided. The DNA sequences may be used in cloning and expression vectors for pro- duction of DNA and RNA and for producing polypeptides including mammalian epidermal growth factor. E. coli strains HB 101 (pmegfl) and HB 101 (pmegfl0b), and bacteriophage lam- bdahEGF34 were deposited at the A.T.C.C. on May 10, 1983 and given Accession Nos. 39357, 39358 and 40070, respectively.

Brent R Larsen, Ned Siegel, Claire E Kotts, Michael F McGrath, Sharon D Ogden, Gwen Krivi, John Minnerly assigned to Monsanto Company

This invention relates to novel peptides having utility for promotion of growth and/or lactation in animals, to processes and DNA useful in pro- duction of such peptides, and to methods utilizing such peptides to promote growth or lac- tation in animals. In some embodiments, the in- vention is directed to peptides having bovine IGF-II biological activity, to production of such peptides, and to their use in effecting prolifera- tion of certain cells (e.g. mammary epithelial or muscle) or in enhancing lactation in cattle or other animals.

4783415

G E N E C O D I N G F O R S I G N A L P E P T I D E S A N D U T I L I Z A T I O N

T H E R E O F

Shiger Hoshiko, Osamu Makabe, Shunzo Fukatsu, Kozo Nagaoka, Yokohama, Japan as- signed to Meiji Seika Kabushiki Kaisha

A DNA segment of a gene coding for a signal peptide is disclosed the segment is to be used in a host-vector system employing a bacterium belonging to the genus Streptomyces as a host by incorporation in the vector DNA. The segment comprises a DNA having a base sequence coding for the peptide having the amino acid sequence from X to Y in the annexed FIG. 1. A vector plasmid to be used for transformation of bac- teria belonging to the genus Streptomyces is also disclosed. The plasmid comprises a DNA seg- ment with a base sequence coding for an extra- cellular alpha-amylase derived from Streptomyces hygroscopicus SF-1084 strain or a mutant thereof the DNA segment incorporated into a vector plasmid is to be used in a host- vector system employing a bacterium belonging to the genus Streptomyces as a host and also having a DNA segment necessary for expression of the gene information of the DNA segment. The present invention may be applied to the pro- duction of a desired protein which will be secreted outside the host microorganism..

4753798

M E T H O D S A N D C O M P O S I T I O N S F O R C O N T R O L L I N G

P R O T O Z O A L I N F E C T I O N S I N W A R M - B L O O D E D A N I M A L S

Sidne Kantor, Robert L Kennett assigned to American Cyanamid Company

This invention relates to methods and composi- tions for the control of protozoal infections in warm-blooded animals by administering to said animals a protozoacidally-effective amount of a new antibiotic designated LL-E19020 alpha, LL- E19020 beta or a pharmaceutically and pro- phylactically acceptable salt thereof. The new antibiotics are produced by microbiological fer- mentation under controlled conditions using a new strain of Streptomyces lydicus subspecies tanzanius or mutants thereof.