Instrumentation, Assay and Equipment Design

4732860

P R O C E S S F O R D E T E R M I N I N G T H E P E R K A L L I K R E I N C O N T E N T

A N D T H E P A R T I A L T H R O M B O P L A S T I N T I M E O F A

P L A S M A S A M P L E

Knut Bartl, Helmu Lill, Wilzhofen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

The present invention provides a process for the photometric determination of prekallikrein in plasma, wherein plasma is incubated with a sur- face activator, the extinction obtained is measured, a chromogenic thrombin substrate is then added thereto, the optically determinable group liberated therefrom is measured at short time intervals or continuously and the gradient of the linear part of the curve obtained by plot- ting the time against the extinction is determined as a measure of the prekallikrein content. The partial thromboplastin time of plasma is simul- taneously determined by carrying out the in- cubation in the presence of calcium ions and a phospholipid and measuring the time it takes the extinction to reach a predetermined level.

4735697

M E T H O D A N D A P P A R A T U S F O R S E P A R A T I N G C O M P L E X

M I X T U R E S O F B I O - O R G A N I C M A T E R I A L S

William G Burton assigned to Phoresis Transfer Systems Inc

A method and apparatus is described for separating complex mixtures of components, particularly bio-organic molecules, preferably

on the basis of two independent physical charac- teristics. A sample initially is resolved in a first stage to yield a fluid stream containing the com- ponents of the sample longitudinally separated. The fluid stream then is aligned incident to the inlet face of a gel slab positioned in a electric field, having a field strength sufficient to force the separated and charged components from the flowing stream into the gel slab substantially simultaneously with exposure of said charged components tO the electric field. Gel elec- trophoresis of the sample can then proceed in a known manner.

4735792

R A D I O I O D I N A T E D M A L E I M I D E S A N D U S E A S A G E N T S F O R

R A D I O L A B E L I N G A N T I B O D I E S

Prem C Srivastava assigned to The United States of America as represented by the United States Department of Energy

A radiopharmaceutical for antibody labeling comprising an iodophenyl maleimide that at- taches thiol sites of proteins thereby maintaining bioreactivity largely located at the nitrogen sites.

4735898

M O N O C L O N A L A N T I B O D I E S A N D M E T H O D O F I D E N T I F Y I N G

S P E C I E S U S I N G T H E S A M E

John C Herr, David C Benjamin assigned to The University of Virginia Alumini Patents Founda- tion

The new method an products recognize human serum albumin, differentiating blood and tissue

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