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4675295

PATENT ABSTRACTS

P R O C E S S F O R P R O D U C I N G S U B C U L T U R A B L E

L Y M P H O K I N E - P R O D U C I N G H U M A N T C E L L H Y B R I D O M A S

Toshiaki Osawa, Yoshiro Kobayashi, Makoto Asada, Masahiro Higuchi, Tokyo. Japan as- signed to Denki Kagaku Kogyo Kabushiki Kaisha

A process for preparing human T cell hybridomas which are subculturable and pro- duce lymphokines comprising the steps of: (1) treating a human acute leukemia cell with a pro- tein and/or RNA synthesis inhibitor: (2) inde- pendently activating a human T cell with a mitogen or antigen; (3) fusing the thus-treated human acute leukemia cell with the thus- activated human T cell in the presence of a fusion accelerator: and (4) isolating the thus-formed hybridoma. An in vivo process for producing lymphokines using the hybridomas is also described.

4675297

G E N E S E N C O D I N G B O V I N E P R O L A C T I N

John D Baxter, Walter L Miller, Joseph A Mar- tial assigned to The Regents of the University of California

A DNA sequence encoding bovine prolactin and optionally including condons for the preceding 10 amino acids is used to construct expression systems to obtain recombinant production of these proteins.

4677054

M E T H O D F O R S I M P L E A N A L Y S I S O F R E L A T I V E

N U C L E I C A C I D L E V E L S IN M U L T I P L E S M A L L S A M P L E S BY

C Y T O P L A S M I C D O T H Y B R I D I Z A T I O N

logical specimens is described. The technique in- volves denaturation of cytoplasmic preparations, followed by dotting of up to 96 samples onto a single sheet of nitrocellulose, hybridization with a 32P-labeled cDNA plas- mid, autoradiography, and scanning. By analy- zing cytoplasmic preparations instead of purified RNA. manipulations of multiple sam- pies prior to analysis are minimized. Experi- ments with a clonal line of rat pituitary tumor (GH3) cells show that this technique can be em- ployed to follow the induction by Ca2£30 +0 + L of prolactin mRNA sequences, employing cytoplasm prepared from as little as 2.5+33 10+ HU 4 + L cells. The specificity of the techni- que for prolactin mRNA is shown by employing GC cells, a GH + HD 3 + L cell variant lacking detectable prolactin mRNA sequences. Experi- ments with cultured rat hemipituitaries show that the prolactin mRNA present in cytoplasm corresponding to as little as 1/100 of a pituitary can be readily detected. This technique is quite simple, can be quantified, and permits the +PG,2 simultaneous analysis of multiple sam- ples while requiring very small amounts of material for analysis. Hence, it should be quite useful for example for studies with various ex- perimental systems of the regulation of specific mRNA levels.

4678751

H Y B R I D H U M A N L E U K O C Y T E I N T E R F E R O N S

David Goeddel assigned to Genentech lnc

Disclosed herein are methods and means of microbially preparing novel human hybrid leukocyte interferons, useful in the treatment of viral and neoplastic diseases, by DNA recom- bination of parental interferon genes, taking ad- vantage of common restriction endonuclease cleavage sites therein and in carrier expression plasmids.

4680176

D E L E T I O N M U T A N T O F A H E R P E S V I R U S A N D V A C C I N E

C O N T A I N I N G S A I D V I R U S

Bruce A White, F Carter Bancroft assigne0 to Sloan-Kettering Institute for Cancer Research

A simple technique for the simultaneous measurement of relative levels of a specific mRNA in numberous small samples of bio-

Antonius J Berns, Arnold L Gielkens, Grave, Netherlands assigned to Centraal Dier- geneeskundig Instituut

The invention relates to live deletion mutants of a herpesvirus, especially of Pseudorabies virus.