PATENT ABSTRACTS 397

to splenocyte-donating mice. Also provided is a method for the biochemical purification of cyto- plasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto. + RE

4670394

I S O L A T I O N A N D C U L T U R E O F A D R E N A L M E D U L L A R Y E N D O T H E L I A L C E L L S

P R O D U C I N G B L O O D C L O T T I N G F A C T O R VI I I :C

4670384

D I A G N O S T I C R E A G E N T F O R S W I N E T R I C H I N O S I S

Howard R Gamble, Kenneth D Murrell as- signed to The United States of America as represented by the Secretary of Agriculture

A hybrid cell formed by the fusion of a mouse spleen cell with a mouse myeloma cell secretes an lgM class antibody. The antibody is useful as a preparative reagent in the isolation of three pro- teins, molecular weights 45,000, 49,000 and 53,000, from the parasite Trichinella spiralis and is also useful as a direct diagnostic reagent is a competitive serodiagnostic test.

Harvey B Pollard, Richar Ornberg, Dipak Banerjee, Moussa Youdim, Peter Lelkes, Eli Heldman assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services

The present invention discloses a new line of endothelial cell of adrenal medullary origin capable of producing blood clotting Factor VIII:C. A method of isolating and culturing said cell line has also been disclosed. Factor VIII:C is useful in treating hemophilia.

4672030

T E S T K I T F O R T H E D E T E R M I N A T I O N O F

A C T I V A T E D T H R O M B O P L A S T I N T I M E ( P T T ) W I T H I N C R E A S E D

S E N S I T I V I T Y T O H E P A R I N

4670386

C A N C E R T E S T S U S I N G T U M O R A N T I G E N G E N E R A T E D

L Y M P H O K I N E S A N D C O M P O S I T I O N S

Stephen Sugaar

The present invention provides strongly anti- genic tumor-specific substances (also cellular material containing said strongly antigenic tumor-specific substances) prepared by radiofre- quency or microwave electromagnetic radiation, acting on cancer tissue or cancer cells of known pathohistologic character. Said substances and also cellular material containing said substances are characterized by such sensitizing antigenic qualities that when they are contacted with a composition of naturally cancer-sensitized lymphocytes taken from a host having patho- histologically related cancer, then said lympho- cytes produce and exude lymphokine. The invention further provides diagnostic tests for cancer and compositions which contain or utilize said strongly-antigenic cancer-specific sub- stances.

Pete Witt, D 7844 Neuenburg, Federal Republic Of Germany

Partial thromboplastin time (PTT) is deter- mined for monitoring and controlling heparin therapy with a test kit having increased sensitivity to heparin. Increased sensitivity is ob- tained by including with reagents in the test kit an organic sulfuric acid or salt thereof such as dodecylbenzenesulfuric acid£13 sodium salt and/or an organic sulfuric acid ester or salt thereof such as +PG,2 sodium laurylsul- fate. + RE

4672053

I M M U N O A F F I N I T Y P U R I F I C A T I O N O F

P H Y T O L A C C I N P R O T E I N S A N D T H E I R U S E IN T R E A T I N G

H E R P E S S I M P L E X V I R U S T Y P E II

Tom G Obrig

Antiviral proteins phytolaccinl and phyto- laccin2 were isolated from the higher plant Phytolaccin americana using the technique of