PATENT ABSTRACTS

base pair recognition site. EDTA-distamycin- Fe(II)(ED.Fe(III)) contains EDTA attached to the carboxy terminus of the groove binder tripeptide, tris-N-methylpyrrolecarboxamide. ED.Fe(II) cleaves DNA contiguous to a five base pair A+30 T rich sequence. +PG,2 Penta- N-methylpyrrolecarboxamide- EDTA.Fe(II)(P5E.Fe(II)) cleaves DNA on opposite strand at the six base pair recognition level in a catalytic reaction. This is the first designed synthetic molecule that approximates the double strand sequence specific cleavage of DNA(4+14 6 bp recognition level) by the natural substance restriction enzymes, tools which make possible recombinant DNA man- ipulations. P5E.Fe(II) cuts DNA at sequences not available by the naturally occurring restric- tion enzymes. The dimers, bis(EDTA- distamycin.Fe(II) and EDTA- bisdistamycin.Fe(ll) w hich double strand cleave DNA at the eight base pair recognition level (A+30 T rich). + REpair A+30 T rich sequence. + PG,2 Pent a-N- methylpyrrolecarboxamide- EDTA.Fe(II)(P5E.Fe(II)) cleaves DNA on opposite strand at the six base pair recognition level in a catalytic reaction. This is the first designed synthetic molecule that approximates the double strand sequence specific cleavage of DNA(4+14 6 bp recognition level) by the natural substance restriction enzymes, tools which make possible recombinant DNA man- ipulations. P5E.Fe(II) cuts DNA at sequences not available by the naturally occurring restric- tion enzymes. The dimers, bis(EDTA- distamycin.Fe(lI) and EDTA-bisdistamycin. Fe(ll) which double strand cleave DNA at the eight base pair recognition level (A + 30 T rich).

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4666829

POLYPEPTIDE MARKER FOR A L Z H E I M E R £ 3 ° S D I S E A S E A N D

I T S U S E F O R D I A G N O S I S

George G Glenner, Caine Wong assigned to University of California

A novel polypeptide, Alzheimer£3 s Amyloid Polypeptide (AAP), is provided in substantially purified form which is isolated from amyloid deposits in patients with Alzbeimer+3 s Dis- ease. The polypeptide has the following amino acid sequence: + R I +TL, I H + H D 2 + L N+13 ASP+ 13 A L A + 13 G L U + 13 PHE+13 A R G + I 3 HIS+I3 ASP+I3 +0 ~ T C +13 SER+13 GLY+13 TYR+13 G L N + 1 3 V A L + I 3 HIS+I3 HIS+13 G L N + I 3 + 0 +13 LYS+13 L E U + I 3 VAL+13 P H E + I 3 PHE+13 ALA+13 G L U + I 3 ASP+I3 +0 + T R +13 V A L + I 3 G L Y + I 3 S E R + I 3 ASN+ 13 LYS+ 13 COOH. + RE+PS + R E + P A The polypeptide, or fragments thereof, may be used to produce antibodies use- ful in a diagnostic test for Alzheimer+ 3 s Dis- ease. Nucleotide probes corresponding to portions of the polypeptide are also useful for diagnostic purposes.

4666836

N O V E L C L O N I N G V E H I C L E S F O R P O L Y P E P T I D E E X P R E S S I O N

IN M I C R O B I A L H O S T S

4666828

T E S T F O R H U N T I N G T O N £ 3 S D I S E A S E

James F Gusella assigned to The General Hospital Corporation

A method for detecting the presence of the gene for Huntington£3 s disease in a subject which comprises analyzing the human chromosome 4 of the subject for a DNA polymorphism, pre- ferably a restriction fragment length poly- morphism (RFLP), linked to Huntington+3 s Disease.

Masayori lnouye, Kenzo Nakamura assigned to The Research Foundation of State University of New York

Methods and compositions are provided for ex- pression of polypeptides in transformed bac- terial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram- negative bacterium, and also linked with an ad- ditional promoter sequence in certain cases. The methods utilize such plasmids to introduce gene- tic capability into microorganisms for the pro- duction of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates there- for.