PATENT ABSTRACTS 301

4661454

1 Y E A S T P R O M O T E R L I N K E D T O N O N G A L A C T O K I N A S E G E N E

David Botstein, Ronald W Davis. Gerald R Fink, Aliso Taunton-Rigby, Robert G Knowl- ton, Jen-i Mao, Donald Moir, Christopher G Goff assigned to Collaborative Research lnc

A DNA segment containing a GALl promoter linked to a gene other than the galactokinase gene for directing the expression of the gene within a yeast cell.

4663015

E L E C T R O P H O R E S I S A P P A R A T U S F O R N U C L E I C A C I D

S E Q U E N C I N G

Donald D Sleeter. Raymond D von Alven, George Fernwood assigned to Bio-Rad Laboratories lnc

An integrated plate structure provides a novel component for use in an electrophoretic sequencing cell. The integrated plate structure consists of a glass plate and a plastic plate bon- ded together along a raised edge of the latter, the edge extending along the sides and bottom of the plastic plate. The plates thus form a permanent enclosure for an upper buffer solution, open at the top for filling. The glass plate further serves as one of the two glass plates forming the gel enclosure.

4663280

E X P R E S S I O N A N D S E C R E T I O N V E C T O R S A N D M E T H O D O F C O N S T R U C T I N G V E C T O R S

Alan Sloma assigned to Public Health Research Institute of the City of New York

The present invention relates to an expression and secretion vector which comprises the signal and promoter sequence of the Bacillus cereus (herein £37 + I B. cereus + 38 + L ) gene which codes for penicillinase and to the construction of vectors and the use of the vectors in the expres- sion and secretion of one or more exogenous polypeptides in microorganisms for example, +I B. subtilis.

4663281

E N H A N C E D P R O D U C T I O N O F P R O T E I N A C E O U S M A T E R I A L S

IN E U C A R Y O T I C C E L L S

Stephen D Gillies, Susumu Tonegawa assigned to Mass Institute of Technology

Disclosed is a method of enhancing expression of recombinant DNA in eucaryotic ceils. A tissue specific enhancer element obtained from the genome of an organism and active in a selected tissue type is combined with a transcriptionally competent transcription unit comprising a pro- moter and exons encoding for the proteinaceous material of interest (or its precarsor). This recombinant DNA is transfected into cells derived from the same tissue as the tissue in which the enhancer element normally functions to enhance expression of endogeneous DNA. The resulting transformants express the exons of the transcription unit at high levels as the en- hancer element increases the copy number of mRNA. The enhancer element operates to in- crease transcription independent of its orientation and position provided it is Iocatcd within an active region on the DNA, generally between about 1£14 10 kilobases (kb) from the 3+40 +0 or 5+40 +0 end of the transcription unit.

4663283

M E T H O D O F A L T E R I N G D O U B L E - S T R A N D E D D N A

Dennis Kleid. Daniel G Yansura, Herbert L Heyneker, Giuseppe F Miozzari assigned to Genentech lnc

A method of altering double stranded DNA comprising converting double stranded DNA to single stranded DNA in a region surrounding a selected cleavage site, hybridizing a compli- mentary primer to the single stranded region for- med in the previous step, and restoring enzymatically the second strand which had been eliminated.

4663290

P R O D U C T I O N O F R E V E R S E T R A N S C R I P T A S E

John Weis, John Salstrom assigned to Molecular Genetics lnc