162

trophoretic mobility within a predetermined range to the total number of the cells to be ex- amined, with the aid of an automatic elec- trophoretic apparatus which is operated in a medium of the physiological conditions and by reversing the electric field, and comparing the obtained pattern of the electrophoretic property of the cells to be examined with the pattern of the electrophoretic property of normal host-cells, in order to diagnose cancer.

4632780

N - T E R M I N A L F R A G M E N T O F H U M A N P R O -

O P I O M E L A N O C O R T I N A N D P R O C E S S T H E R E F O R

Nabil G Seidah, Michel Chretien, lie des Soeurs, Verdun, Quebec, Canada

There are disclosed the N-terminal fragment of human pro-opiomelanocortin, a glycopeptide composed of 76 amino acid residues, and a pro- cess for preparing same from human pituitary glands. The glycopeptide is useful in potentiating the effects of ACTH on steroidogenesis, in stimulating the production of aldosterone, as a diagnostic tool, as well as a reagent for deter- mining its presence in biological fluids and tis- sues by immunochemical means.

4632902

M E T H O D F O R D E T E C T I N G B I O L O G I C A L A C T I V I T Y IN A

B O D Y F L U I D

John Waters. Rodne Broman assigned to Becton Dickinson and Company

A method of detections biological activity is dis- closed. A nutrient growth medium which isolates antibiotics and other microbial growth in- hibitors during culturing of a microorganism. The growth medium includes an aqueous disper- sion of nutrient materials and an effective amount of an isolating substance or substances capable of isolating antimicrobial materials during culturing of a microorganism. The isolating substances are selected from ion ex- change resins and non-functional adsorbent resins.

PATENT ABSTRACTS

4634663

M E T H O D A N D R E A G E N T F O R Q U A N T I T A T I V E

D E T E R M I N A T I O N O F N- A C E T Y L H E X O S A M I N E

Tatsuo Horiuchi, Noda, Japan assigned to Noda Institute for Scientific Research

A method for quantitative determination of N- acetylhexosamine which comprises (a) allowing an N-acetylhexosamine oxidase to act upon an N-acetylhexosamine-containing liquid in the presence of oxygen and then (b) determining the amount of oxygen consumed in the oxidation reaction or the amount of hydrogen peroxide or N-acetylhexaminic acid formed in the oxidation reaction. Further, a reagent for quantitative determination of N-acetylhexosamine, charac- terized by containing an N-acetylhexosamine oxidase.

4634675

A G I T A T O R F O R A F E R M E N T A T I O N A N D T I S S U E

C U L T U R I N G V E S S E L

David Freedman, Zheng Zhenbin, Highland Pk, N J, United Statee assigned to New Brunswick Scientific Co Inc

An improved agitator for use with a fermenta- tion and tissue culturing vessel acting as a micro- carrier culture vessel is provided. The agitator includes a hollow body assembly having an open end and a closed end and at least one tubulation communicating with the hollow body assembly at a location spaced from the open end thereof, the tubulation having an exit opening oriented such that movement of the agitator and the tubulation causes a suction or draft at the exit opening to cause flow of fluid from the open end of the hollow body assembly through the hollow body assembly and out through the tubulation exit opening. In this way. a microcarrier cell cul- ture may be stirred in a smooth and non-erratic way thereby minimizing the detachment of the rounded growth cells from the microcarriers.

4634676

R E P L I C A P L A T I N G D E V I C E

Bruno V Sapatino assigned to Becton Dickinson and Company

PATENT

A replica plating device for use with an open- ended container which holds a plurality of cells on a culture medium therein comprises a sub- stantially planar pressing member. A side wall extends upwardly from the pressing member and surrounds same. A skirt is connected to the side wall and extends downwardly from its connec- tion point in the direction toward said pressing member along the exterior surface of said side wall in spaced relation thereto. As a result, the side wall and skirt form a hook for maintaining the device in position on the container to allow limited, controlled movement of the device for replica plating of cells within the container. A method of using a device substantially as described above for obtaining print-replicas of cells or the like is also within the purview of the present invention.

4636463

A N T I B O D I E S T O H U M A N I N T E R L E U K I N - 2 I N D U C E D B Y S Y N T H E T I C P O L Y P E P T I D E S

Amnon Altman, Argyrios N Theofilopoulos, Richard A Lerner, Frank J Dixon assigned to Scripps Clinic and Research Foundation

Chemically synthesized polypeptides containing about 6 to about 40 amino acid residues having amino acid residue sequences that substantially correspond to the amino acid residue sequences of antigenic determinants of interleukin-2, when administered alone or as polymers or as con- jugates bound to carriers, induce the production of antibodies of predetermined specificities. The polypeptides and the antibodies produced thereto can be used in diagnostic systems to measure the presence and amount ofinterleukin- 2 and interleukin-2 cell surface receptors or bi- nding sites in an assayed sample.

4636464

P Y R A N O S E O X I D A S E , M E T H O D O F M A K I N G A N D M E T H O D O F

U S E

Toru Nakanishi, Yozo Machida, Atsugi, Japan assigned to Kyowa Hakko Kogyo Co Ltd

A novel pyranose oxidase exhibiting the strong specific activity only on glucose is produced by fermentation of a microorganism of the genus Coriolus, Daedaleopsis, Gloeophyllum or Pleurotus. The enzyme is useful for the deter- mination of glucose.

ABSTRACTS

4636465

M E T H O D A N D C O M P O S I T I O N F O R D E T E R M I N A T I O N O F

G L Y C E R O L

Nobuya ltoh, Kuniyoshi Matsunaga, Suzuka, Japan assigned to Amano Pharmaceutical Co Ltd

A method for determination of glycerol by using a reagent system comprising glycerol dehy- drogenase and a pyridine nucleotide coenzyme, characterized in that an enzyme selected from the group consisting of triokinase and dihydrox- yacetone kinase is incorporated into the reagent system to eliminate the formed dihydrox- yacetone or D-glyceraldehyde in the assay sys- tem.

163

4 6 3 ~ 7 2

D I S P O S A B L E S T E R I L I Z A T I O N B I O L O G I C A L T E S T P A C K

Loran H Bruso assigned to Warner-Lambert Company

A disposable sterilization test pack for deter- mining the el~cacy of sterilizing equipment com- prises a porous base pad having a cavity for receiving the sterilization indicator. Porous pads are disposed on opposite sides of the base pad to retain the sterilization indfcator within the cavity, and an impermeable layer is disposed against one of the porous pads to confine the in- troduction and evacuation of air from the test pack to the edges of the test pack and the ex- posed porous pad which is not covered by the impermeable layer.

4636473

M E M B R A N E A N D S T A T I C M I X E R - M O D E R A T E D

B I O R E A C T O R W I T H A N T I - F O U L I N G D E V I C E

Clement Kleinstreuer assigned to Rensselaer Polytechnic Institute

A bioreactor arrangement comprises a housing defining a closed space having a longitudinal axis with at least two spaced apart microporous membranes fixed therein dividing the space into a first chamber on one side of one of the mem- brane, a second chamber on one side of the other membrane, and a third chamber between op- posite sides of the one and other membrane. The housing includes at least one port corn-