4483920

I M M O B I L I Z A T I O N O F M E S S A G E R N A D I R E C T L Y F R O M C E L L S

O N T O F I L T E R M A T E R I A L

David Gillespie, Isadore Brodsky, Joel Bresser assigned to Hahnemann University

Message RNA is immobilized directly from cells onto filter material. Immobilization is carried out by solubilizing cellular components with a chaotropic salt, passing the resultant solubilized cellular components through a filter which selec- tively binds message RNA and baking the filter containing bound message RNA. The chaotro- pic salt is preferably sodium iodide, potassium iodide or sodium perchlorate. Prior to solubilizing, the cells may be washed and lysed. The bound message RNA can be hybridized to a labeled probe and the amount of message RNA measured. Prior to baking, the filter containing bound RNA may be incubated in a solution which acelylates basic protein and other molecules which might interfere with molecular hybridization.

4486533

F I L A M E N T O U S F U N G I F U N C T I O N A L R E P L I C A T I N G

E X T R A C H R O M O S O M A L E L E M E N T

Alan M Lambowitz assigned to St Louis Univer- si ty

A functional extrachromosomal element capable of replication in filamentous fungi is provided. The extrachromosomal element em- ploys (1) a mitochondrial replicating element or (2) a lower organism replication sequence recognized by the fungus, in combination with foreign DNA to provide replication, transcrip- tion, and translation of foreign regulatory ele- ments and genes. The extrachromosomal element is exemplified by a mitochondrial replicating system from Neurospora. The cell strain E. coli HBI01 containing the plasmid pALS-1-1 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39157. The cell strain E. coli HB101 containing the plasmid pALS-2 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39158.

4487835

P L A S M I D H A V I N G T E M P E R A T U R E D E P E N D E N T

P L A S M I D C O P Y N U M B E R

PATENT ABSTRACTS 87

Bernt Uhlin, Kurt Nordstrom, Soeren Molin, Petter Gustaffson, Odense, Denmark assigned to A/S Alfred Benzon

Gene products of plasmid DNA, such as pro- teins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a con- trolled constant plasmid copy number at one temperature and a much higher or totally uncon- trolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy num- ber pattern.

4489160

P L A S M I D P C G 2

Ryoich Katsumata, Tetsuo Oka, Akira Furuya, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd

~ K y r I

Restriction Number of enzyme cleavage sites

Hindnl 3 Kpnl 2 SamHl 1 Pstl 1 Hpal 1 EcoRI 0 Sail 0 BglU 0

Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.

~ 9 ~ 7 2

S E N S I T I V E T E S T S F O R M A L I G N A N C I E S B A S E D O N D N A

D E T E C T I O N

A Arthur Gottlieb assigned to Imreg Inc

An improved, more convenient, more sensitive test for detection of certain malignancies in human and animal subjects is disclosed. Sera from test subjects is mixed with labeled DNA in the presence of an enzyme-conjugated resin. Sera from normal and cancerous subjects react dif- ferently with the resin, permitting a diagnosis of the subject.