308 PATENT ABSTRACTS

Flavobacterium heparinum (ATCC 13125) or mutants thereof having activity to reduce the coagulation activity of factor X while not ef- fecting the coagulation activity of thrombin.

4396713

R E S T R I C T I O N E N D O N U C L E A S E F I N G E R P R I N T I N G O F

K I N E T O P L A S T D N A M I N I C I R C L E S

Larry P Simpson, Carlos M Morel assigned to The Regents of the University of Calif

Hemoflagellates are characterized by restriction endonuclease digestion of the mitochondrial DNA to provide for substantial cleavage of the kDNA network. The resulting electrophoretic profile of the digest can be used as a restriction fingerprint for distinguishing organisms and specific strains. The kDNA is found to be suffic- iently stable through numerous passages of the organism to provide a reliable fingerprint.

~ 9 6 ~ 2

S T R A I N S O F Y E A S T F O R B R E A D - M A K I N G A N D N O V E L S T R A I N S

O F Y E A S T T H U S P R E P A R E D

Philippe Clement, born Hennette Annie Loiez, Roubaix. France assigned to Lesaffreet Cie

A complete and reproducible process for pro- ducing novel strains of yeast, comprises making a first SCreening test and at least one other screening test selected from a group of screening tests which do not resort to any measurement of gas release, selecting by means of said first and at least one other said screening test the desired strains from a group of diploid strains prepared previously either by hybridation, or by mutation of existing strains. The tests are as follows: A first test consists of measuring the average multiplica- tion coefficient of a given strain by following the optical density variation of a standard medium seeded by a suspension of cells obtained from

this strain. A second test consists of measuring in the same manner the average multiplication coefficient of the said strain in the presence of an inhibitor acid added to the standard medium. A third test consists of measuring the maltose adaptation of said strain in the presence of glucose by determining the amount of maltose subsisting in a standard medium after a known amount of glucose added to this medium has been completely consumed. A fourth test con- sists of measuring the invertase content of said strain. A fifth test consists of measuring the la- tent time of said strain. The hybridation can con- sist of systematic haploid crossings derived from quick Saccharomyces Cerevisiae strains adapted to maltose and haploids derived from very slow strains not adapted to maltose, but well adapted to sweet doughs and sometimes also to acid doughs.

4396609

A M I N O C Y C L I T O L A N T I B I O T I C S , P H A R M A C E U T I C A L

C O M P O S I T I O N S A N D M E T H O D O F U S I N G S A M E

Sol J Daum, Robert L Clorke assigned to Ster- ling Drug Inc

Aminocyclitol analogs ofgentamicin CI, C2 and C la and the corresponding compounds acylated on the I-, 3- and 2'-amino groups with an omega- amino- alpha-hydroxy-lower-alkanoyl group are prepared by culturing a nutrient medium containing carbohydrates, a source of as- similable nitrogen, essential salts and an added aminocyclitol with a mutant of Micro- monospora purpurea and acylating the product with an ester of an omega-(N- benzyloxycarbonyl)amino- alpha-hydroxy- Iower-alkanoic acid followed by catalytic hydrogenolysis of the benzyloxycarbonyl group.