Upload
juan
View
233
Download
0
Embed Size (px)
Citation preview
8/11/2019 4-9 Mitochondrial DNA.pdf
1/16
DNA in the nucleus
DNA
Contains our genetic information
8/11/2019 4-9 Mitochondrial DNA.pdf
2/16
Two kinds of DNA within our cell
DNA
Within the nucleus
Within the mitochondria
1
2
8/11/2019 4-9 Mitochondrial DNA.pdf
3/16
Circular genome
Mitochondrial DNA (mtDNA)
16 569 bases
37 genes
Non-coding regions known as D-loop
1 to 3% variation betweenunrelated individuals
8/11/2019 4-9 Mitochondrial DNA.pdf
4/16
High copy number per cell
Mitochondrial DNA (mtDNA)
More resistant to sample degradation
More robust than nuclear DNA
8/11/2019 4-9 Mitochondrial DNA.pdf
5/16
Mitochondrial DNA (mtDNA)
mtDNA inherited strictly from the mother
Not unique to an individual
Faster mutation than nuclear DNA
8/11/2019 4-9 Mitochondrial DNA.pdf
6/16
The Roma
8/11/2019 4-9 Mitochondrial DNA.pdf
7/16
Russias imperial family
The Romanovs
1917 Russian revolution
1918
Romanov family murdered / executed
8/11/2019 4-9 Mitochondrial DNA.pdf
8/16
The Romanovs
Remains burned and buried
No nuclear DNA
mtDNA extracted
8/11/2019 4-9 Mitochondrial DNA.pdf
9/16
Romanov family tree
8/11/2019 4-9 Mitochondrial DNA.pdf
10/16
The Romanovs
Intact maternal line
Same mtDNA as Tsar Nicholas
One set of bones identified
8/11/2019 4-9 Mitochondrial DNA.pdf
11/16
The Romanovs
Remains of the Tsars children
Same mtDNA as the Tsarina
8/11/2019 4-9 Mitochondrial DNA.pdf
12/16
Romanov family tree
8/11/2019 4-9 Mitochondrial DNA.pdf
13/16
The Romanovs
Remains were that of the Romanov fa
Prince Philip should have thesame mtDNA as the Tsarina
mtDNA matched the remains
8/11/2019 4-9 Mitochondrial DNA.pdf
14/16
The Romanovs
Rumors
Did the Grand Duchess
Anastasia survive?
Grand Duchess An
8/11/2019 4-9 Mitochondrial DNA.pdf
15/16
8/11/2019 4-9 Mitochondrial DNA.pdf
16/16
DNA analysis
Tissue from a surgical sample
DNA extracted
Match to Franziska Schanzkowska