3D Printed Tracheal Graft: Early Results in a Porcine Model

Faiz Bhora, MD, FACSChief Thoracic Surgery

Co-Director Airway Center

Mount Sinai St. Luke’s and Roosevelt Hospitals

Associate Professor of Thoracic Surgery

Department of Thoracic Surgery

Icahn School of Medicine at Mount Sinai

Disclosures

• Royalties: Merit Endoteck• Consultant: Boston Scientific, CSA Medical• Research Materials: TEI Biosciences

Background

• Increasing application of 3-D printing and 3-D bioprinting

in Medicine

• The ability to produce a customized organ derived from

biocompatible material or from patient derived stem cells

is the new frontier in biomedical engineering

• We aim to bioengineer a customized tracheal graft that

would reliably repair an anterior tracheal defect

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Model

• Graft composed of Extracellular Matrix (ECM), Bioplastic and Mesenchymal Stem Cells

• Bioplastic: Polycaprolactone (PCL). FDA approved for human implant. Hydrolyzed over time.

• Stem Cells: Human Mesenchymal Stem Cells, primed to differentiate into chondrocytes in a bioreactor

• 3D Printing: Used for graft customization

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Model

3D Printer

HMSCs

Defect 3 cm

How We Do It

* *

6.2 cm

The Graft

3D Printed Graft Incubated Grafts with Stem Cells

How We Do It

Bronchoscopy 3 Months Post-Op

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Summary

• n =3, 100% survival• Patent airway, no malacia• Minimal granulation tissue• Appears to be well vascularized• Good mucosal coverage• Graft supports animal growth

Mount Sinai / Presentation Slide / October 27, 2014 11

Future

• Biomaterials• Stem cell •Longer, more complex grafts

Acknowledgments

Mount Sinai Department of Thoracic Surgery Reseach Lab

Faiz Bhora, MD - PI

Raja Flores, MD - Chairman

Adnan Al-Ayoubi, MD PhD

Sadiq Rehmani, MD

Craig Forleiter, MD

Ahmad Altaweel, MD

Michael Barsky, BA

ENT

Robert Lebovics, MD

Catherine Sinclair, MD

Animal Care Facility and Department of Comparative Medicine

This work was supported by MSSL Associate Trustees Fund to FYB and AMA, and the Hussein Family Fund to FYB

DAPI DEAD LIVE

PCL - Biocompatible for HMSCs Growth

Chondrogenesis Is a Complex Orchestrated Process

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