3520

Relationship of characteristics of enhanced-ultrasonography andbiology of hepatocellular carcinomaLin L, Lin X-Y, Xue E-S, He Y-M, Gao S-D, Yu L-Y, Lin X-D, UnionHospital of Fujian Medical University, China

Objectives: To probe the relationship between the characteristics ofenhanced-ultrasonography and biology of hepatocellular carcinoma(HCC).Methods: Clinically proved 26 HCC and eight focal nodule hyperpla-sia (FNH) were examined by double real-time gray scale contrast-enhanced ultrasound with an intravenous administration of SonoVue.All the cases were taken aspiration biopsy for pathological histologicalexamination and FCM DNA analysis. According to the show of con-trast enhancement, the cases of HCC were divided into two groupswhich were typical group with enhancement beginning time �20' andfadeaway beginning time �50' and nontypical group with fadeawaybeginning time �50'. The results of enhanced-ultrasonography werecompared with those of DNA analysis.Results: Twenty-six HCC included 18 cases which were vested intypical group and eight in nontypical group. Eight FNH were all vestedin nontypical group. DNA analysis showed S period and G2/M ratios ofHCC which were vested in typical group were 37.6% � 7.3% and14.0% � 2.1%, respectively, markedly higher than those of HCC,which were vested in nontypical group (23.4% � 6.7% and 8.4% �1.7%) (p � 0.05) and those of the latter were also higher than those ofFNH (2.3% � .5% and 1.6% � 0.4%) (p � 0.01). Aneuploid peakappeared in 30% (6/18) HCC which were vested in typical group anddidn’t appeared in nontypical group and FNH. One HCC in typicalgroup with diameter 5.2 cm showed only a nodule with diameter 0.9 cmappeared “quick enhancement and quick fadeaway” and the other partdid not appeared enhancement. These two parts were all HCC inpathology examination, while the nodule part appeared aneuploid peakand the other only showed diploid in DNA analysis.Conclusions: DNA analysis shows high proliferation state in HCCwhich are vested in typical group and low or no proliferation state innontypical group and FNH, which indicated ultrasonic contrast showsnot only reflect the hemodynamics change of HCC but also relates to itscharacteristics, which deserved further study.

3521

Contrast-enhanced sonography for evaluation of solid-organinjury with active bleeding in blunt abdominal traumaZhou X, Li Y, Luo W, Zheng M, Ren X, Zhang J, Fourth MilitaryMedical University, Xijing Hospital, China

Objectives: To evaluate contrast-enhanced sonography (CES) in thediagnosis of solid-organ injuries with active bleeding in blunt abdom-inal trauma.Methods: Self-made miniature impactor was used to make models ofsolid-organ injury with active bleeding in blunt abdominal trauma in 10pigs and 20 dogs. Conventional 2D-sonography(2DS) and CES wereused to detect local echo changes of the livers, spleens or kidneysbefore and instantly after impacting. After experiments the organs weretaken out for gross pathologic examination and HE staining.Results: The sonographic pattern of lesions in the animals’ organs at2DS was hypoechoic in various degrees or anechoic against normalparenchyma with poorly-defined margins. CES showed the radiateenhancement of vessels were interrupted in the lesion areas in arterialand portal phase in liver. The microbubbles flowed from the ruptures toblood accumulation like “pouring ppring” or “spurting”. During thedelayed phase the enhanced echogenicity of the normal parenchymabegan to weaken, whereas that of lesions weakened slowly with clear

borders against normal parenchyma due to contrast agent. Gross patho-logic findings were consistent with the CES results (p � 0.05).Conclusions: CES can observe solid-organ injury with active bleedingin blunt abdominal trauma in real time, which is valuable to furtherclinical treatment.

3522

Preliminary study of SonoVue in contrast-enhanced sonographyLiao S-R, Wu W, Dai Y, Yang W, Chen M-H, Oncology School ofPeking University, China

Objectives: To observe perfusion characteristic of contrast agentSonoVue, and investigate the role of SonoVue in differentiating benignand malignant tumors.Methods: Thirty-three ovary tumors (16 benign, 17 malignant) wereexamined with contrast agent SonoVue and contrast tuned imaging(CnTI) real time gray scale ultrasound technique. Perfusion patterns oftumors, arrival time, peak time and the duration of contrast agent effectwere recorded and analyzed.Results: After contrast agent administration, for benign tumors, twocases with no enhancement, 13 cases with slight enhancement incapsule, septum or interior parts. Solid parts in all malignant tumorsand one benign tumor enhanced obviously. The enhancement peakintensity was higher (36.8 versus18.4, p � 0.001), the arrival time wasshorter (13.5 seconds versus 17.5 seconds, p � 0.023), and the durationof contrast agent effect was longer (254.7 seconds versus 208.5 sec-onds, p � 0.04 in malignant tumors than they were in benign tumors.There was no significant difference in peak time between malignantand benign tumors.Conclusions: SonoVue can assess precisely vascular pattern of ovarytumors. There are significant differences in the arrival time, enhance-ment peak intensity, and the duration of contrast agent effect.

3524

Intrahepatic collateral and hemodynamic changes in chronicBudd Chiari syndrome: Doppler and echo-enhanced sonographyfindingsMarcelino A, Pinho M, Vianna P, de Oliveira A, Saito O, Caiado A,Chammas M, Bacchella T, Machado M, Cerri G, Institute ofRadiology, Clinical Hospital, University of Sao Paulo, Brazil;Department of Surgery and Liver Transplantation, Clinical Hospital-University of Sao Paulo, Brazil

The aim of this study is to review the morphologic findings and thehemodynamic changes in chronic Budd Chiari Syndrome (CBCS) byDoppler sonography and echo enhanced contrast agents. Althoughvenography is the gold standard for BCS, sonography is an importantimaging tool for diagnosis and follow-up. Echo-enhanced contrastagents are available and have different applications on liver studies.Mechanisms of hemodynamic compensation include increase in arterialflow, redistribution of portal flow from areas where outflow is impairedtoward areas where outflow is preserved, and development of small orlarge venous collaterals bypassing the obstructed veins. Chronic Budd-Chiari is more difficult to diagnose because the clinical findings arevariable, depending on the number of hepatic veins affected and theability of the liver to develop collaterals. Echo-enhanced contrastagents are a promising tool that may assist B-mode and Dopplersonography on diagnosing Budd-Chiari syndrome and its complica-tions.

Abstracts P281