F/IOBMBAPPLICATION FORM

YSP-Travel Fellowships for l3th FAOBMB Congress, Bangkok,23-29 November, 2012.

Name of Applicant: Dr, Wiraya Srisimarat

Position: Postdoctoral Fellow (career stage)

Address ofApplicant:Department of Biochemistry, Faculty of Science, Chulalongkom University254 Phayathai Road, Pathumwan, Bangkok Thailand. 10330Tel: 662-218-5428

Email address: wiraya.s@gmail.com

Membership of FAOBMB Constituent SocietylBiocherristry and Molecular Biology Section, Science Society ofThailandhttp://www.scisoc.or.th/

Date of Birth: 2 June 1982

Applicants must be no more than 35 years ofage at the closing date for application:(31 .ruly,2012).

ProofofAge: (Certification by the Head of your Institution or Department is required)

I ceftify on the basis ofofficial documentation which I have seen (e.g. passpofi, birthcertificate or driver's licence) that the date ofbidh ofthe applicant Dr. Wiraya S simaratis 2'"' June 1982, as shown above.

. . -Signed: ( i . /acsz.^-Z-L '

Position: Head of Depa(ment

Have you previously received an FAOBMB Travel Fellowship? Ifyes, please indicatewhen.

- I have never received an FAOBMB Tmvel Fellowship.

Page 1 of 6

Curriculum VitaeNdme I Wiraya SrisimaratBon r 2 June 1982; Bangkok, ThailandDegrce i20ll Ph.D.(Biochemistry),ChulalongkomUniversity,Thailand2006 M.Sc.(Biochemistry),ChulalongkomUniversity,Thailand2003 B.Sc. (Biotechnology), King Mongkut's Institute ofTechnology

Ladkrabang, ThailandActieities :Participant of FAOBMB Micro-Syhposium 2012, June 1, 2012, Chulalongkom

Univercity, Bangkok, Thailand.Conduct expe ment "Crystal structure of carbohydrate modifying enzymes"

(Proposal No. 20],2-2-064-I), May 19-21,2012, National Synchroton RadiationResearch Center. Hsinchu. Taiwan. R.O.C.

Participant of 6'' ,4siak Cyclodexttitx Confercnce, AnEtJst 28-31,201 1, The AustralianNational University, Canbelra, Austalia, (Poster presentation)

Participant of RGJ-Ph.D. Congress XII, April l-3, 2011, Chonbu , Thailand (Oralpresentatlon).

Participant of The 1"' Joint Symposium CU-.VUI December 23-24, 2010,Chulalongkorn University, Thailand. (Poster presentation)

Participant of The 15'' International Cyclodextrin Symposium, May 9-12, 2010,Vienna, Austria. (Poster presentation)

Research visit, January 12 - July.1,2010. Leipzig University, Leipzig, Germany.Staff and chair person of the l4tk Biological Sciences Graduate Congress, December

l0-12, 2009. Chulalongkom University, Bangkok, Thailand.Participant of Kick-offworkshop: Novel amylomaltase for the slnthesis of large-ring

cyclodextrin, October 13-15, 2008. Leipzig University, Leipzig, cermany. (Oralpresentation)

Research visit, October 12 - November 9,2008. Leipzig University, Leipzig,Germany.

Participant of the l"' Biochemistry and molecular biologt conference: Biochemistryand molecular biology for the Integration o;f life, April 26-27, 2007.Chulalongkom University, Bangkok, Thailand. (Poster presentation)

Staffof rie 5" JSPS-NRCT Joint Seminar on Microbial Resources, November 7-10,2006. Pattaya, Thailand.

Grunls, Fellotoships and Awaruls :2011-present Postdoctoral Fellowship under the Ratchadaphiseksomphot

Endowment Fund, Chulalongkom University2011 Best Poster Presentation - Gold Award ftom 6' Asian Cyclodextrin

Conference, August 28-31, 2011, The Australian National Unive$ity,Canbena. Austalia.

2010 TeachingAssistantFellowship2008 Research visit grant for I month from Research Institution Pannership Crant

from Alexander von Humboldt Foundation200'l-2011 The Royal Golden Jubilee Ph.D. Fellowship ffom Thailand Research Fund2006 Graduate Thesis Grant from Graduate School, Chulalongkorn University2005 TeachingAssistantFellowship2003 The Best Activist Award from Faculty ofScience, King Mongkut's

Institute of Technology Ladkrabang2002 Tokai-KMITL lnternship crant from Tokai University and King Mongkut's

Institute of Technology Ladkrabang

Page 2 of 6

List of Publications in International Journals** Nunber all publications in intematiohal journals ol book chapters, \)ith earliest first andmost receht qt lhe end. Protide the Impact Factot (lF) ofthe journal and indicate yourcontribution the vork described and the publication. Use as many pages as required. Followthe style in these exdmples:

Publication :

2.

3 .

4.

5.

1 . W. Srisimarat and P. Pongsawasdi. Effect of cyclodext n on oligosaccharidesynthetic activity of beta-galactosidase. ln Proceedings of the First Biochemistryand Molecular Biology (BMB) Conference: Biochemistry and Molecular Biologtfor I he integrutioh of life , April 26-27 , Thailand. pp. 1 22- I 3 0. (2007)WS's contributioh to this wo* was iwestigating efect of cyclodextrin on aci,,)ityof B-galactos idase and prepared I he manuscript.

W. Srisimarat and P. Pongsawasdi. Enhancement of the oligosacchaddesynthetic activity of B-galactosidase in organic solvents by cyclodextrin. Ehzymeand Microbial technologt 43,436-441 (2008) OF 2.367)WS's contribution to this work was in.t)estigatihg efect of cyclodextrin on activityoJ p-galactosidase and ptepared the manuscript.

W. Srisimarat, A. Powviriyakul, J. Kaulpiboon, K. Krusong, W. Zimmermannand P. Pongsawasdi. A novel amylomaltase ftom Corynebactetiun glutamicumand analysis of the large-ring cyclodextrin prodtcts" Journal of InclusionPhenomena and Macrocyclic Chemistry70, 369-375 (2011) 0F 1.886)WS's cohtribution to this work was cloning, expression and characterization ofamylomaltase including yoduction of large-ring cyclodextrin. lls preparcd themanuscript.

W. Srisimarat, J. Kaulpiboon, K. Krusong, W. Zimmermann ald p.Pongsawasdi. Mutation at Tyr-l'12 in the amylomaltase from Corynebacteriumglutamicum leads to a change in large-ring cyclodextrin products ptofile, AppliedEwirohhetal Mcrobiolog) (2012) (Acaepted) OF 3.829)II.S's conlribution to this work was mutatioh, expression and characterization ofatnylotualtase and mutant including produclion of large-ring cyclodextik. llspr e par e d t he m anuscript.

S. Saehu, W, Srisimarat, M.H. Prousoontom and P. Pongsawasdi.Transglucosylation reaction of amylomaltase for the slnthesis of anticariogenicoligosaccha des. Journal of Molecular Catalysis B: Enzymatic (2012)(submitted) (IF 2,735)lfs's contribution to this wotk was analysis oligosaccharide product andsuggestion some the experimental setup.

Page 3 of 5

AbstractIndicate below the abstract that is submitted by you for presentation at the ysp and theFAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text oftheabstract)

CRYSTALLIZATION OF AMYLOMALTASE FROMCo 4u e bacl e fi um glutamic um

Srisimarat, W.r, Krusong, K.r and Pongsawasdi. p.r' Depaftment of Biochemistry, Faculty of Science, Chulalongkom University,Bangkok, Thailand

Email address: wiraya.s@grnail.com

Amylomaltase (EC 2.4.1.25) ftom Corynebacterium glutanicum is a gl kDa proteinwhich belongs to the 4-o-glucanotransferase (4ocTase) family. This enzymecatalyzes the tansfer of o-1,4-D-glucan units via an intemolecular andintramolecular transglucosylation reaction to produce longer chain oligosaccharidesand cycloamyloses, tespectively. In order to understand the mechanisms of Cglutdmicum amylomaltase (CgAM) at molecular level. the three-dimensional strucluleanalysis is essential. The CgAM has been cloned and exprcssed in Escherichia ColiBL21(DE3)'. However, the amino acid sequence of C.gAM is 28oZ and 33oZ simlar rothose of amylonaltases ftom Thermus aquaticus, rlird, Solanum tube osums . of whicbtheir crystal structutes have already been solved. Crystallization of CqAM has beenobtained by the hanging-drop vapour-diflusion nretbod. preLiminary d;1a shows thatthe crystal diffiacts to 2.5 A at synchrotron source INSRRC. Taiwan). However, themolecular-replacement method does not provide corect solution for strucruredetermination. Therefore, tlte selenomethionine (Se-Met) substituted protein crystalsare rcquired. The C'gAM was expressed in E colt 8834(DEj) that is a MethionineAuxotroph strain. We found that this enz),rne displays normal disporportionauonactivity on maltotriose substate. The maxjmunr specific acri\,ity is oblain;d when .lerecombinant cell was cultured at 37 oC for 4 hours after induced with 0.4 mM IPTG.Pudfication of Se-Met CgAM is now in progress.

Kelrvords: Amylomaltase, Corynebacterium, structure detemination. MethronmeAuxotroph StrainReferences1 Srisimarat, W., Kaulpiboon, J., Krusong, K., ZiDrmermanr W., pongsawasdi, p.

(2012) Mutation at Tyr-172 in the amylomaltase from Corwebacternmglutamicum leads to a change in large-ring cyclodextrin products profile, Appl.Environ. Micro. (Accepted)

2. Przylas, I., Tomoo. K., Terada, Y., Takaha, T., Fujii, K., Saenger, W., Stater, N.(2000) Crystal structure of anylomaltase from Thermus aquaticur, aglycosyltransferase catalyzing the production of large cyclic glucans, J. Mol.B io l .296,873-886.

3. Imamura, K., Matsuura, T., Takaha, T., Fujii, K., Nakagawa, A." Kusunoki, M.,Nitta, Y. (2004) Structure detemination and refinement at 1.g A resolution ofDispropoftiomting enzyme for potato. [Online]. Available trom:http://w$v.rcsb.org/pdb/explore/explore.do?structureld:lX1N [2012, May 30]

Page 4 o f6

Personal StatementIndicate briefly here your Research Interests and Career Coals, why you are interestedto participate in the YSP Program (including what you will bring to the YSP and whatyou hope to gain from it): (no more than q49 page)

The biochemistry, enzymology and molecular biology are interesting andimportant fields for life science development today. The scope of these fields alsooffers significant intellectual stimulation and challenge. These concems have mademe as a bachelor in Biotechnology Aom King Mongkut's Institute of TechnologyLadkabang choose the department of Biochemisty, Faculty of Science,Chulalongkom University for my gmduate studies. I have joined the "Starch andCyclodextrin Research Unit" Laboralory which is specialized in the reseaxch area ofstarch metabolism, cyclodextrin producing and carbohydmte-modifying enzymes.

My Ph.D. rcsearch focuses on the molecular studies of bacterialamylomaltase, a large- ng cyclodextrin producing enzyme. Cloning, characterizationof the enzyme and site-directed mutagenesis for identifying residue involved inenzyme catalysis were performed. In my 1" year of Postdocteral training, expressionof the enzyme in yeast which is a GRAS organism for applicable use in food hasbeen focused. Due to the potential advantage of amylomaltase in industry, mypresent work involves stuctural determination ofthe enzyme by x-ray crystallizationto disclose basic knowledge in this enzyme which will lead to more understanding ofits mechanism. To proficient in an)'thing, it requires extensive study and endlesspractice, sometimes even life-long.

In my opinion, the Youlg Scientist Progran is a meaningful program foryoung researcher in Biochemistry and Molecular biology areas. It is a geatopportunity for many ofthe young PhD students or Postdocs to present their researchworks and discuss about the new findings, sharing their lab experiences andknowledge to each other. Not ody an academic program, the participants will have achance to have activities together which might provide collaboration and networkingin the future. Finally, I believe the YSP is the right specialty for me and I am excitedto participate in this program.

Page 5 of 6

sf

AlEshrsslE.

. Letters of recommendation from lgq referees.

Submission methods: You cal apply in either oftwo ways:

. by sending an email with scanned attachments to the Chair ofthe FAOBMBFellowship Committee, Prof. Piamsook Pongsawasdi:piarnsook.p@chula.ac.th or-pi4qlqsk p@glqailqqq

This is the prefened method. lf using this method, please assemble the ApplicationFoIm and the Attachments into a litglq PDF file.

. by sending a hard copy ofthe application fo1m and supporting documents to:

Professor Piamsook Pongsawasdi,Department of Biochemistry, Faculty of Science,Chulalongkorn University,Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7Hours). Applicants will be notified by email ofthe decision ofthe Committee by no laterthan 15 September, 2012.

Page 6 o f6

Department of BiochemistryFaculty ofScience

Chulalongkorn UniversityBangkok 10330

30 htly,2012

To whom it may concem,

It gives me great pleasure to recommend Dr. Wiriya Srisimarat as a candidatetoioin the FAOBMB Young Scientist Program (YSP) which will be held during thel3'" FAOBMB Congress in Bangkok in Novembe\2012.

Dr. Wiiya Srisimarat received her Master and Ph.D in Biochemistry fromDepadment of Biochemistry, Faculty of Science, Chulalongkorn University. Thus, Ihave known her for nearly 7 years. She was one of out top students with highmotivation. I also found her ar industdous young researcher with high responsibility.After her Ph.D study, she has received a postdoctoral Fellowship under theRatchadaphiseksomphot Endownment Fund from ow university to continue herrcsearch under the supervision ofProf. Piamsook Pongsawasdi,

Besides her strong academic background and research experience, Dr. WiriyaSrisimarat speaks fluelt English and has ftiendly personality that instantly attractsncw friends. She also exhibits temendous enthusiasm in leaming new things as sheactively participates in different student activities in our depaxtment. I confident thatDr. Wiriya Sdsimarat will make a good contribution to the success of the YSP andjoining the Program will give her a great opportunity to meet renowned scientists aswell as young scientists of her age from different countdes that could promoteresearch collaborations and will be a great beDefit for her future career-

Yours Sincerely,

I . '2,t*-z-'

Professor Dr. Alchalee TassanakajonHead of DepartmentE-mail: anchalee.k@chula.ac.th

Department of BiochemistryFaculty of ScienceChulalongkom UniversityBangkok 10330, Thailand.

July 28,2012

TO WHOM IT MAY CONCERN

- . As the supervisor of Dr.Wiraya Srisimar-a! I am writing to support herintention to participate in the FAOBMB young Scientist program 2012. iitronglybelieve she will perform very well and lake a good role during the program.

Dr.Wiraya Srisimarat is presently a postdoctoral researchir oithe Starch andCyclodextrin Research Unit at the Department of Biochemistry, Faculty of Sorence,Chulalongkom University. She had enrolled in our graduate program of

'Biochemistry

under my guidance and received the M.Sc. in 2006 and ph.D. in 201l. Dudne herPh.D. study, she received the scholarship liom the Thaitand Research Fund, shi hadspent half a year doing research at the University of Leipzig, Germany. She haspresented her work at many Conferences, both national and intemational. In 201 l, shereceived the Best Poster Presentation Gold Award from the 6th Asian CyclodextrinConference in Canberra, Aushalia.

. Dr.- Srisimarat is a very active young researcher with high capability andpotential. for research work. During the last few years, she his worked quiteeffectively and has helped a lot in training younger and newcomer gmduate and alsoundergraduate students in my laboratory, She really works hard

-and shows great

rntention to develop expertise in new techniques that she has just leamed. She has agood nature with leadership and always willing to help. It is tirerefore with my grearpleasure to highly recommend her for the ySp fellowship.

Sincerely Yours,

P. h"*-/-Piamsook Pongsawasdi, Ph.D.Professor in Biochemistry