2545 Binary Gradient Module Operator’s Guide · 2007-11-21 · Gradient Module, or the 3100 Mass Detector) supports no more than two vertically oriented “accessory” Waters modules

Waters 2545 Binary Gradient Module

Operator’s Guide

71500134902 / Revision A

Copyright © Waters Corporation 2006 All rights reserved

Copyright notice

© 2006 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

TrademarksWaters is a registered trademark, and AutoPurification, FractionLynx, and MassLynx are trademarks of Waters Corporation.

Tefzel and Teflon are registered trademarks of E. I. du Pont de Nemours and Company.

Other trademarks or registered trademarks are the sole property of their respective owners.

Customer commentsWaters’ Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at [email protected].

Waters Corporation34 Maple StreetMilford, MA 01757USA

ii

Safety considerations

Some reagents and samples used with Waters instruments can pose chemical, biological, or radiological hazards. Be sure you are aware of the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization’s safety representative for guidance.

When you develop methods, follow the “Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory,” American Journal of Medical Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating procedures and the techniques necessary to validate system and method performance.

Instrument-specific safety considerations

High voltage hazard

Safety advisoriesConsult Appendix A for a comprehensive list of warning and caution advisories.

Warning: To avoid electric shock, do not remove the 2545 Binary Gradient Module’s protective panels. The components they cover are not user-serviceable.

iii

Operating this device

When operating this device, follow standard quality control procedures and the guidelines presented in this section.

Intended useWaters® designed the 2545 Binary Gradient Module to manage solvents under MassLynx™ software control in Waters AutoPurification Systems.

CalibrationTo calibrate HPLC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should cover the entire range of quality-control samples, typical specimens, and atypical specimens.

To calibrate mass spectrometers, consult the calibration section of the operator’s guide of the instrument you are calibrating.

Quality controlRoutinely run three quality-control samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that quality-control sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when quality control samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily.

Warning: Although some of the modules in the AutoPurification System might carry IVDD labels, the system as a whole is not intended for clinical use.

iv

Table of Contents

Safety considerations ......................................................................................... iii

Operating this device ......................................................................................... iv

1 Setting up the 2545 Binary Gradient Module .................................. 1-1

Features .............................................................................................................. 1-2

Installing the binary gradient module ........................................................ 1-4

Unpacking and inspecting .............................................................................. 1-6

Making power connections ............................................................................. 1-7

Making plumbing connections ...................................................................... 1-9

Making signal connections ........................................................................... 1-16

2 Preparing for operation ....................................................................... 2-1

Powering on ....................................................................................................... 2-2

Scanning connected instruments .................................................................. 2-3

Configuring 515 pumps .................................................................................... 2-6

Creating the inlet method ............................................................................... 2-8

Defining the gradient ..................................................................................... 2-10

Entering timed events ................................................................................... 2-14

Setting up analog signals .............................................................................. 2-16

Preparing solvent reservoirs ....................................................................... 2-17

3 Maintenance ............................................................................................ 3-1

Maintenance considerations .......................................................................... 3-2

Removing the pump head and seal-wash assemblies ............................... 3-4

Replacing the seals and O-ring .................................................................... 3-10

Table of Contents v

Replacing the plunger .................................................................................. 3-16

Replacing an inlet check valve cartridge ................................................. 3-20

Replacing an outlet check valve cartridge ............................................... 3-22

Replacing the vent valve rotor-seal assembly .......................................... 3-24

Diagnostics and configuration .................................................................... 3-28

Performing the leak test ............................................................................... 3-29

4 Troubleshooting ..................................................................................... 4-1

System troubleshooting .................................................................................. 4-2

Alarm reset ......................................................................................................... 4-4

Error messages .................................................................................................. 4-5

Troubleshooting hardware ............................................................................. 4-8

A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2

Caution symbol .................................................................................................. A-6

Warnings that apply to all Waters instruments ......................................... A-7

Electrical and handling symbols ................................................................. A-14

B Specifications ........................................................................................ B-1

Physical specifications ................................................................................... B-2

Environmental specifications ....................................................................... B-3

Electrical specifications ................................................................................. B-4

Solvent management system specifications .............................................. B-7

Index ..................................................................................................... Index-1

vi Table of Contents

1 Setting up the 2545 Binary Gradient Module

Contents:

Topic Page

Features 1-2

Installing the binary gradient module 1-4

Unpacking and inspecting 1-6

Making power connections 1-7

Making plumbing connections 1-9

Making signal connections 1-16

1-1

Features

The Waters® 2545 Binary Gradient Module is a high-flow, high-pressure mixing binary-gradient pump that delivers solvent for the Waters AutoPurification™ System.

Based on Waters high-performance solvent management technology, the binary gradient module provides consistently smooth, pulse-free solvent flow for both analytical and preparative flow rates. The binary gradient module is capable of pumping four solvents. Only two solvents can be programmed for any gradient.

Pump systems A and B

Each binary gradient module consists of two independent pump systems, A (top) and B (bottom). Each independent pump system contains two linear-drive actuators (left and right). Each left and right actuator pair contributes equally (in parallel) to deliver precise flows of a single solvent.

A

B

1-2 Setting up the 2545 Binary Gradient Module

The two independent pump systems combine their two solvents at a filter/tee, where the solvent mix is plumbed to the sample manager or another component of the AutoPurification System.

MassLynx™ software controls the ratio of the two solvents by varying the relative flows of pump A and pump B. The binary gradient module receives pressure data from a pressure transducer associated with each pump head. The firmware measures the individual pump head pressure during the pumping cycle and adjusts the precompression to ensure consistent solvent delivery and minimize pump-induced detector baseline disturbances.

The MassLynx interface with the binary gradient module also provides useful diagnostics and troubleshooting routines.

Leak managementAll fluid-handling areas of the binary gradient module contain leak routing capability. Spilled solvent is routed to the drain tube below the front panel.

The optional leak sensor should be installed on the leak sensor bracket in the drip tray.

See also: Waters AutoPurification System Guide and Waters System Fluidics Organizer Operator’s Guide.

Features 1-3

Installing the binary gradient module

To install the binary gradient module, you should know how to set up and operate general laboratory instruments and computer-controlled devices, and how to handle solvents.

See also: Contacting Waters Technical Service on page 3-2.

Required materials

• Flat-blade screwdriver (included in Startup Kit)

• 1/2-inch open-end wrench (included in Startup Kit)

• 5/16-inch open-end wrench (included in Startup Kit)

Site requirements

Factor Requirement

Internal cooling DC-powered fans that provide internal cooling by pulling air through the binary gradient module and exhausting it out the rear of the unit.

Airflow requirements Air enters the front of the binary gradient module so pumps and other instrumentation can be placed side by side.

Clearance At least 6 inches (15 cm) between the rear of the binary gradient module and the wall to allow for electrical connections and the ventilation fans’ exhaust.

Operating temperature 4 to 40 °C (39.2 to 104 °F)

Humidity 20% to 80%, noncondensing

Instrument-generated acoustic noise

<60 dBA at idle

Height 17 inches (43 cm)

Width 11 inches (28 cm)

Depth 31 inches (79 cm) with cables connected at the rear; includes heads and tubes at the front

Weight 125 pounds (57 kg)

Line voltage 100 to 240 Vac ±10 V


Frequency 50/60 Hz

Current requirements, fusing

10 A, 5 × 20 mm, slow-blow, IEC type

Power consumption 650 W

Warning: System configurations are designed to meet safety and performance standards. All systems must adhere to a model in which any one “base” Waters module (i.e., the System Fluidics Organizer, the 2545 Binary Gradient Module, or the 3100 Mass Detector) supports no more than two vertically oriented “accessory” Waters modules (e.g., 515 pumps, photodiode array detectors, evaporative light scattering detectors, or dual wavelength absorbance detectors). Configuring modules in such a way that fails to meet said design is neither recommended nor supported by Waters Corporation. When configurations require module stacking outside the noted parameters, the customer assumes liability.

Site requirements (Continued)

Factor Requirement

Installing the binary gradient module 1-5

Unpacking and inspecting

The binary gradient module is shipped in a single carton on a wooden pallet. Save the carton and pallet in case you transport or ship the unit later.

To unpack and inspect the binary gradient module:

1. Ensure that there is sufficient bench space for the binary gradient module.

2. Check the outside of the shipping carton for signs of damage. Document or photograph areas of damage.

3. Remove the plastic wrap, if any, and the bands securing the carton to the pallet. Remove the top and the sides of the shipping carton, the Startup Kit, and the packing material.

4. Check that the contents of the Startup Kit match the Startup Kit list.

5. Remove the binary gradient module from the bottom styrofoam cushion and the box bottom, and place it on a level, clean surface, making sure to leave room for the other system components.

6. Check that the instrument serial number (found on the rear panel near the power cord connector) corresponds to the number on the Certificate of Validation.

7. If you detect any damage to the contents of the carton, refer to Shipments, Damages, Claims, and Returns, in Waters Licenses, Warranties, and Support Services.

8. Measure the voltage at the receptacle, and verify that the voltage is between 90 and 264 Vac.

Caution: To prevent damage, lift the module by the bottom sides of the unit.

Warning: To avoid injury, ensure that at least two people lift the module from the pallet to the bench. Most of the module’s weight is at the front.

!


Making power connections

Requirements: Make sure the AC power supply is grounded and has no abrupt voltage fluctuations.

The binary gradient module automatically adjusts for AC input voltages of 100 to 240 V, 50/60 Hz.

To connect the binary gradient module to the power source:

1. Ensure that the power switch on the front of the binary gradient module is in the Off position.

2. Connect the female end of the power cord to the power entry module on the rear of the binary gradient module.

Caution: Do not power on the binary gradient module until you have completed all plumbing and signal connections.

Warning: To avoid electrical shock and possible injury, remove the power cord from the rear panel of the instrument before you perform this procedure.

!

Making power connections 1-7

Rear panel of 2545 Binary Gradient Module

3. Insert the other end of the power cord into a grounded power receptacle.

Key

Number Name

1 Power entry module

2 Fuse holder

3 Ethernet RJ45 port

4 Connector A

5 Connector B

3

4

5

2

1


Making plumbing connections

Installing solvent inlet tubes

Required materials

• Solvent inlet tubes assembly (part number 430000573)

• 1/2-inch wrench

Recommendation: Perform a dry prime before making the initial plumbing connections (see page 2-18).

To make the initial plumbing connections:

1. Using a 1/2-inch wrench, loosen the solvent inlet fitting on the manifold.

2. Slide the nut and ferrule retainer, followed by the ferrule (small end toward the end of the tube) onto the solvent tube.

3. Press the tube all the way into the fitting and tighten the nut using the 1/2-inch wrench. Tighten the fitting 3/4 of a turn beyond finger tight.

4. Repeat steps 1 to 3 for the remaining solvent tubes.

Caution: Check all fittings and tighten if necessary. The fittings can loosen during shipment, particularly if the module has undergone temperature extremes.!


Plumbing connections for seal wash

Key

Number Name

1 Seal-wash pump A

2 Seal-wash A inlet tube

15

12

3

2

1

10

13

11

8

4

9

14

5

6 7

1 2

1 2


If you plan to run only two solvents, make sure to plumb one solvent into each manifold and connect the tube to a port in each manifold.

You can shorten the solvent inlet tubes to accommodate the solvent container locations.

5. Insert the filter ends of the solvent tubes into the appropriate solvent container.

3 Filter

4 Seal-wash B outlet tube

5 Drip tray

6 Drain tube

7 Inlet manifold B

8 Seal-wash B connection tube

9 Seal-wash B inlet tube

10 Vent valve

11 Seal-wash pump B

12 Waste outlet at tee fitting

13 Seal-wash A outlet tube

14 Seal-wash A connection tube

15 Inlet manifold A

Caution: The binary gradient module does not require pressurized solvent containers for reliable operation. If using pressurized containers for blanketing large containers of solvent, do not exceed 5 psig. Pressures above 5 psi can force solvent through the internal components of the solvent selection valve (SSV), possibly causing a spillover into the solvent waste reservoir or an adverse mixture with other solvents.

Key (Continued)

Number Name

!


Installing seal-wash solvent supply tubesEach seal-wash solvent supply tube is pre-assembled with a free-sliding PEEK compression screw, stainless steel collar, and yellow ferrule. Be careful not to lose these parts when working with these tubes.

Required material

Seal-wash inlet tube assembly (part number 430000576)

To install the seal-wash solvent supply tubes:

1. Insert the seal-wash solvent supply tube into the top port of the pump A seal-wash pump. Ensure the tube is pressed into the pump before tightening the compression screw to set the ferrule.

Caution: Do not re-use the solvent inlet tube ferrule more than two times. Re-use may result in slow leaks. Cut the end of the tube squarely and use a new ferrule. The nut can be re-used.

Warning: To avoid possible harmful vapors, position solvent containers in a safe place, such as in a fume hood or under a counter vented to the outside.

!


Installing the seal-wash solvent supply tubes

2. Install a second tube to the pump B seal-wash pump.

Installing the seal-wash waste tube

Required material

Seal-wash waste tube assembly (part number 430000575)

To install the seal-wash waste tube:

1. Remove the black cap from the end of the tube and install the tube into the seal-wash outlet port on the right side of the right pump A head.

Caution: To properly drain the waste fluid, ensure that the waste tube has no crimps or bends. A crimped or bent drain tube may prevent adequate flow to the waste container.!


Installing the seal-wash waste tube

2. Repeat step 1, but install the compression screw into the left side of the left pump B head.

Installing the vent valve waste tube

Required materials

• Waste tube assembly (part number 430000574)

• 5/16-inch open-end wrench

To install the vent valve waste tube:

1. Install the waste tube to the waste outlet at the T-fitting (to the right of the vent valve).

2. Install the free end of the tube in the waste container.

Installing the drip trayThe drip tray is mounted at the bottom of the binary gradient module bezel.

The optional leak sensor should be installed on the leak sensor bracket in the drip tray.

See also: Waters AutoPurification System Guide.

Caution: To properly drain the waste fluid, be sure to secure the end of the waste tube in the waste container.!


Required materials

• Drip tray (part number 700001514)

• Waste tube, 1/4-inch

• Leak sensor (optional; with leak detection module)

To install the drip tray on the binary gradient module:

1. Clip the drip tray into the bezel.

2. Slide the 1/4-inch ID waste tube over the barbed drain fitting.

3. Place the free end of the drain tube in an appropriate waste container, and ensure that the drain tube has no crimps or bends.

Installing the system outlet tube

Required materials

• System outlet tube assembly (part number 430000574)

• 5/16-inch wrench

To install the system outlet tube:

1. Insert the system outlet tube assembly into the system outlet and tighten with a 5/16-inch wrench.

Installing the system outlet tube

2. For a binary gradient module used as a chromatographic pump, connect the system outlet tube between the inline filter and the appropriate port on the sample manager.


Making signal connections

The rear panel of the binary gradient module provides connection terminals and communication ports for operation with external devices. The signal connections you need to make to the binary gradient module depend on the instruments that constitute your system.

This section describes the input/output (I/O) and digital signal connections that you can make from the terminal strips and communications connectors.

To make the signal connections on the binary gradient module:

1. Connect one end of the Ethernet cable to the RJ-45 connector on the rear panel of the binary gradient module. Connect the other end of the cable to an empty socket on the Ethernet switch.

2. Connect the event input and event output cables from the customer-defined external device (for example, column-switching or fraction collector) to the appropriate connector on the rear panel of the binary gradient module.

3. For mass spectrometer-based systems, connect one end of a signal cable to the stop flow terminals of the connector strip on the mass detector. On connector B at the rear of the binary gradient module, remove the jumper and connect the other end of the cable to terminals 6 and 7 on connector B.

To install optional leak detection, the Waters field service engineer:

• Removes the jumper installed across the terminals 6 and 7 on connector B, Leak Detector Input.

• At the rear of the system fluidics organizer, connects three individual wires at one end of the sensor module cable to SFO LEAK DET Out (+) and the LEAK DET (-) on the terminal strip.

• Connects the other end of the cable to the 2545 binary gradient module LEAK DET (+) and (-) terminals (terminals 6 and 7 on connector B on the rear panel of the binary gradient module).

I/O signal connectionsThe rear panel includes two removable connectors that hold the screw terminals for I/O signals. These connectors are keyed so that they can be inserted only one way.


The terms “Connector A” and “Connector B” are generic and do not refer to pumps A and B.

I/O signal connectors

I/O signals

Signal Description

Switch 1 to Switch 4(A1, A2, A3, A4, B1, B2, B3, and B4)

See Event switches on page 1-18.

Ground (A5, A10, B5, and B10)

Connected to signal ground and used as reference for outputs.

Gradient Start (A6 and A7)

An input that initiates the pumps to begin gradient operation by either contact closure input or 0 volt input.Connect the positive input wire to the start gradient (+) terminal and the negative input wire to the start gradient (–) terminal.

1 Switch 1 (Out) 2 Switch 1 (Out) 3 Switch 2 (Out) 4 Switch 2 (Out) 5 Ground 6 Gradient Start + (In) 7 Gradient Start – (In) 8 Stop Flow + (In) 9 Stop Flow – (In) 10 Ground 11 Chart Out 1 + (Out) 12 Chart Out 1 – (Out)

1 Switch 3 (Out) 2 Switch 3 (Out) 3 Switch 4 (Out) 4 Switch 4 (Out) 5 Ground 6 Leak Detector + (In) 7 Leak Detector – (In) 8 Auxiliary + (In) 9 Auxiliary – (In) 10 Ground 11 Chart Out 2 + (Out) 12 Chart Out 2 – (Out)

Connector A (top) Connector B (bottom)

Jumper


Event switchesYou can use the eight terminals (switches 1 through 4 on connectors A and B) as contact-closure switches to control column-switching valves, fraction collectors, or similar external devices. Each pair of switch terminals can be connected to an external device. You can operate the switches:

• manually from the Direct Functions screen.

• automatically in a separation method using a timed table (I/O screen).

Stop Flow(A8 and A9)

An input that enables other LC components to stop the solvent flow from the binary gradient module. You can specify the signal conditions (using the Events In screen key in the Configuration screen) that stop the flow. Connect the positive input wire to the Stop Flow+ terminal and the negative input wire to the Stop Flow– terminal.

Leak Detector (B6 and B7)

Two terminals (+, –) that allow other devices to immediately stop solvent flow. You can stop flow on the high or low signal.Input voltage range: ±30 Vdc.Logic high = >3.0 Vdc ±10%. Logic low = <1.9 Vdc ±10%.Minimum pulse width = 10 msec.The binary gradient module is shipped with a jumper installed across the terminals of the Leak Detector Input. The binary gradient module cannot start flow if this jumper is missing, unless a properly functioning Waters Leak Detector is connected.

Auxiliary Input (B8 and B9)

Reserved for future use.

I/O signals (Continued)

Signal Description


Event switch positions and functions

Position Function

On Closes the switch.

Off Opens the switch.

Toggle Changes the current state of the switch.

Pulse Closes/opens the switch for a user-defined period.

No Change Leaves the switch in its current state.



2 Preparing for operation

MassLynx software controls the components of the AutoPurification System. This chapter explains how to configure the binary gradient module as a chromatographic (system) pump in the AutoPurification System.

Contents:

Topic Page

Powering on 2-2

Scanning connected instruments 2-3

Configuring 515 pumps 2-6

Creating the inlet method 2-8

Defining the gradient 2-10

Entering timed events 2-14

Setting up analog signals 2-16

Preparing solvent reservoirs 2-17

2-1

Powering on

When you have completed all electrical, plumbing, and signal connections, you are ready to power on the 2545 Binary Gradient Module.

To power on the binary gradient module, move the power switch (at the upper-right corner of the front panel) to the I (On) position.

At startup, the binary gradient module runs through a series of diagnostic tests that home each actuator, check instrument connections, and verify signals from each transducer.

Example: Front panel of binary gradient module

If the system includes one or more 515 pumps, ensure that all electrical, plumbing, and signal connections are complete, and then power on the 515 pumps.

See also: 515 HPLC Pump Operator’s Guide.

Caution: Do not power on the binary gradient module until you have completed all electrical, plumbing, and signal connections. !

2-2 Preparing for operation

Scanning connected instruments

The pump control module in the system fluidics organizer controls any 515 pumps in the system. Scanning the instruments in the system retrieves serial numbers and ensures communication with MassLynx.

See also: System Fluidics Organizer Operator’s Guide.

To scan the instruments:

1. From the main menu, click Start > Programs > MassLynx > MassLynx V4.1, or double-click the MassLynx icon on the desktop.

Opening MassLynx

2. In the Instrument tab, click (Inlet Method).

Opening the inlet method

3. Click (Inlet).

See also: “Setting up instruments” in FractionLynx Help.


Viewing the inlet method

4. Click , and then select Configure Waters Pump Control Modules.

See also: “Configuring Waters Pump Control Modules” in FractionLynx Help.


Waters Pump Control Configuration dialog box

5. Click Scan.

If scanning does not complete in a few minutes, ensure that:

• The cables are correctly and securely attached.

• The on/off switches are set to On.

• Communication settings are correctly specified.

See also: “Setting up instruments” in FractionLynx Help.

6. After all devices complete the scanning process, click Close.

7. For Pump (1), the chromatographic pump, select the 2545 Binary Gradient Module serial number in the “Associate with an instrument” list.

8. Click OK to save the scanned information.

9. If the system does not have any 515 pumps, select LC > Reset Communications, continue with Creating the inlet method on page 2-8.

If the system has one or more 515 pumps, continue with Configuring 515 pumps on page 2-6.


Configuring 515 pumps

To configure a system that has one or more 515 pumps:

1. Check Enable for 515 (A), 515 (B), and/or 515 (C). They can function either as contributors to solvent flow A or B, or as non-contributors.

2. Select the GPIB address (for the pump control module) in the “Associate with an instrument” list.

Tip: You can change the pump names in the Module Name column.

3. Under Configure, click the ellipsis “...” (Browse) in the 515 (A) row.

Selecting 515 pump heads and flow contributions

4. For each 515 pump, select the size of the installed pump head.

Values: 50, 100, or 225 µL. The default is 100 µL.

5. Select the chromatographic flow contribution for each 515 pump.

Values: None (a makeup pump), %A (contributes to flow from BGM pump A), or %B (contributes to flow from BGM pump B).

6. Click OK.

7. Select LC > Reset Communications, and then click Close.

8. Click Inlet Method > (515 Pumps).


Defining 515 pump parameters

9. For each 515 pump, enter the initial flow rate, and select the solvent name. The default flow rate is 1.00 mL/min.

Tip: You can change the low and high pressure limits for each 515 pump.

10. Click OK.

Requirement: For each 515 pump that is not a contributor to the system flow (makeup pump), in the Events table, you must program the flow rate, start flow, and stop flow events.

Configuring 515 pumps 2-7

Creating the inlet method

To create the inlet method for the binary gradient module:

1. In the Modify Instrument Method screen, enter a value in Run Time. Run Time represents the total time of the experiment: the time from the injection of a sample into the system until all chromatography and data acquisition for that sample are complete.

Recommendations: The run time you enter here applies only to the 2545 Binary Gradient Module. You must specify a run time for each module in the AutoPurification System. Run times are typically the same for all modules and are determined from your Standard Operating Procedures (SOP) or method development.

Modifying an instrument method

2. In the Solvent Selections area, select the solvents you plan to run (A1 or A2, and B1 or B2).


3. In the Solvent Names area, enter names for Solvent A and Solvent B. To change solvent names, enter a name for solvent A in the label A box and enter a name for Solvent B in the label B box.

Examples: acetonitrile, methanol, water.

4. In the Pressure Limits area, enter the low-pressure limit for the system in the Low field. If the system pressure falls below this limit, the flow stops and the LC Status error light turns red.

Range: 0 to 41,400 kPa (414 bar, 6000 psi). The default is 0 psi.

5. Enter the high-pressure limit for the system in the High field. If the system exceeds this limit, the flow stops and the LC Status error light turns red.

Range: 0 to 41,400 kPa (414 bar, 6000 psi). The default is 6000 psi.

6. In the Seal Wash Period field, enter the time interval between seal-wash pump pulses. The seal wash lubricates the plunger and flushes away solvent or precipitated salts forced past the plunger seal from the high-pressure side of the solvent piston chamber.

Range: 0.5 to 9999 minutes. The default is 5 minutes.

Creating the inlet method 2-9

Defining the gradient

The gradient table controls solvent flow and composition during each sample run. The Curve column indicates the gradient curve’s profile.

Example: The curve specified in row 2 runs in the interval between gradient event 1’s initial conditions and gradient event 2’s initial conditions.

To define the gradient:

1. In the Modify Instrument Method > Chromatographic Pump > Mobile Phase tab, enter the gradient event 1 information in the table in the lower portion of the Modify Instrument Method screen.

2. In the first row of the Flow column, enter the solvent flow rate at the start of the run.

3. You can enter either %A or %B. In the %A or %B column, enter the percent solvent at the start of the run.

Range: 0 to 100% in 0.1% increments.

The software automatically calculates the other % composition so that %A plus %B equals 100%.

4. In the Curve column, enter the gradient curve profile. Curve profiles can indicate a change in conditions at start (1), a convex curve (2 through 5), linear (6), a concave curve (7 through 10), or a change to final conditions at end (11).

Gradient curve profiles

Final conditions

Initial conditions

Start time End time


5. In the next row of the Time column, enter the run time at which you want gradient event 2 to start.

6. In the Flow column, enter the solvent flow rate you want the pump to reach by the start of gradient event 3.

7. In the %A or %B column, enter the percent solvent composition you want the pump to reach by the start of gradient event 3.

8. In the Curve column, enter the curve profile for gradient event 2 to follow while reaching the start of gradient event 3.

9. Repeat steps 5 through 8 for all gradient events your application requires. You can define up to 50 gradient events.

10. If the system has 515 pumps as contributors and you want to know the calculated 2545 BGM flow rate without 515 pumps, select Pump Only.

Result: The flow rate of the 2545 BGM pump appears in the Flow column. This estimates the flow rate on the 2545 BGM’s front panel. The %A and %B composition of the gradient are also calculated, based on the contributions of the 515 pumps.

Example:

Entering the 515 flow rates is shown in Defining 515 pump parameters on page 2-7.

• The flow rate for 515 (A) pump is 1.00 mL/min, and it does not contribute to solvent flow.

• The flow rate for the 515 (B) pump, At-Column Dilution, is 1.25 mL/min, and it contributes to solvent flow B.

• The total flow rate for the System is 25.00 mL/min.


Entering the total system flow rate


• To display the flow rate of the BGM alone, click Pump Only. In this example, the system calculates the flow rate of the 2545 BGM as 23.75 mL/min.

Calculating the flow rate of the 2545 BGM (Pump Only)

11. When you are finished setting up the method, click OK.


Entering timed events

To enter the timed events:

1. In the Modify Instrument Method screen, click the Events tab.

Entering timed events

2. In the Initial Switch States area, set the Switch 1 state. You can set the four event switches to the states you want at the start of each run.

Valid switch states

State Description

No Change Keeps the switch at its current state.

Toggle Changes the switch to the opposite state.

Pulse Transmits a single toggle pulse. The contact closure is maintained for the number of minutes that you set in the Param field. The range is 0.01 to 100.00 minutes.


3. Select Run Events.

4. From the Vent Valve list, select System. The vent valve facilitates priming and purging of solvent lines and changing solvents.

5. In the Events table, in the first row of the Time column, enter the run time at which you want the first timed event to occur. Enter or select the switch that you want to execute the event.

6. In the Action column, enter or select the action that you want the switch to perform.

7. For each pulse (a single toggle), in the Param column, enter the number of minutes you want to maintain the contact closure.

8. Repeat steps 5 through 7 for the remaining timed events in your application. You can define up to 50 timed events.

On Turns on a contact closure that triggers an external or internal event. With this function, the contact closure remains closed until an Off function is sent by the software.

Off Turns off the contact closure for the event. With this function, the contact closure is broken.

Valid switch states (Continued)

State Description

Entering timed events 2-15

Setting up analog signals

You can set up output analog signals to record system information.

Requirement: Analog signals require a Waters eSAT/IN Module in the system.

See also: Waters e-SAT/IN Module Installation Guide.

To enter analog information:

1. In the Modify Instrument Method screen, click the Analog tab.

2. Select the optional analog signals from the Chart Out 1 and Chart Out 2 drop-down lists, and then click OK. Valid signals are %A, %B, Pressure, or Flow Rate.

Tip: The full scale output voltage is 2.0.


Preparing solvent reservoirs

Tip: When operating at high flow rates, and/or with viscous solvents, you can remove the solvent reservoir filters if you need to reduce flow restriction.

Priming the plunger-seal-wash pumpThe plunger-seal-wash solvent lubricates the binary gradient module plungers and flushes away solvent or precipitated salts forced past the plunger seal from the high-pressure side of the solvent piston chamber.

Required materials

• Tube adapter (see startup kit)

• 30-mL syringe (see startup kit)

• Seal-wash solution

Ensure the plunger-seal-wash supply tube is in the plunger-seal-wash solvent container and that the plunger-seal-wash waste tube is in an appropriate waste container.

To prime the plunger-seal-wash:

1. Connect the tube adapter to the syringe.

2. Fill the syringe with plunger-seal-wash solution, then connect the syringe assembly to the plunger-seal-wash inlet tube.

3. In the MassLynx inlet status page, select the Additional Status tab, and then select the Chroma button.

Warning: The binary gradient module does not require pressurized solvent containers for reliable operation. If using pressurized containers for blanketing large containers of solvent, do not exceed 5 psig. Pressures above 5 psi can force solvent through the internal components of the solvent selection valve (SSV), possibly causing a spillover into the solvent waste reservoir or an adverse mixture with other solvents.


Opening the Diagnostics Chromatographic Pump dialog box

4. Select Diagnostics > Seal-Wash Prime. The seal-wash pump starts priming.

5. Press the plunger on the syringe to dispense the seal-wash solution.

6. To stop the priming procedure, select Diagnostics > Seal-Wash Prime.

7. Remove the syringe and adapter, and then place the plunger-seal-wash inlet tube into the purge/plunger-seal-wash solvent reservoir.

Performing a dry primeAt startup, or if the check valves and seals dry out and are sticking, perform a dry prime.

Required materials

• Syringe adapter

• 30-mL syringe (see Startup Kit)

• Priming solution


To perform a dry prime:

1. Fill the syringe with priming solution.

2. Remove the inlet tube and nut from the inlet manifold.

3. Screw the nut from the priming syringe into the port that you want to prime.

4. Inject the solvent into the manifold.

5. Remove the syringe and reinstall the inlet tube.

Priming the binary gradient module

To prime the binary gradient module:

1. On the front panel, press the Prime button. This action pulls solvent through the four pump heads, primes the A1 and B1 solvent inlet tubes, and then primes the pump.

2. To prime other solvent inlet tubes or modify solvent flow rates, in the MassLynx inlet status page, select the Additional Status tab, and then select the Chroma button.

3. In the Diagnostics Chromatographic Pump dialog box, select Diagnostics > Wet Prime.


Selecting the solvents for priming

4. Select the solvents you plan to run. Choose solvent A1 or A2, and then choose solvent B1 or B2.

5. In the Flow Rate (mL/min) field, enter the solvent flow rate for the priming run.

6. In the Time (min) field, enter the number of minutes for priming.

7. Click Prime. The values you enter in the Flow Rate (mL/min) and Time (min) fields become the defaults for the binary gradient module.

8. To stop priming, press the Stop button on the front panel.

Priming continues until you press the Stop button.


3 Maintenance

Contents:

Topic Page

Maintenance considerations 3-2

Removing the pump head and seal-wash assemblies 3-4

Replacing the seals and O-ring 3-10

Replacing the plunger 3-16

Replacing an inlet check valve cartridge 3-20

Replacing an outlet check valve cartridge 3-22

Replacing the vent valve rotor-seal assembly 3-24

Diagnostics and configuration 3-28

Performing the leak test 3-29

3-1

Maintenance considerations

Safety and handlingObserve this warning when you perform maintenance procedures on your binary gradient module.

Proper operating proceduresTo keep your binary gradient module performing properly, follow the operating procedures and guidelines.

Spare partsWaters recommends that you replace only parts mentioned in this document. For spare parts details, see the Waters Quality Parts Locator on the Waters web site's Services/Support page.

Contacting Waters Technical ServiceIf you are in the USA or Canada, contact Waters Technical Service (800 252-4752). If you are not in the USA or Canada, visit http://www.waters.com and click About Waters > Worldwide Offices, or phone your local Waters subsidiary or Waters’ corporate headquarters at 34 Maple Street, Milford, MA 01757, USA.

When contacting Waters, have the binary gradient module serial number available.

Warning: To prevent injury, always observe good laboratory practices when you handle solvents, change tubes, or operate the binary gradient module. Know the physical and chemical properties of the solvents you use. Refer to the Material Safety Data Sheets for the solvents in use.

3-2 Maintenance

Seal lifeSeal life of three to six months or longer is possible, but is highly dependent on the following:

• Mobile phases and modifiers used

• Flow rate

• Use of seal-wash

• Back pressure

• Cleanliness of the mobile phases

• Usage

When to perform maintenancePerform maintenance procedures when you discover a problem with a specific component in the binary gradient module. For information about isolating problems in the binary gradient module, see Troubleshooting.


Binary gradient module components

Component Function

Vent valve Allows priming and venting.

Seal-wash pumps Provides solvent to wash the plungers and plunger seals.

Pressure transducer Senses back pressure developed by resistance to the solvent flow.

Inline filter Provides solvent filtering between the binary gradient module and the sample manager.

Maintenance considerations 3-3

Removing the pump head and seal-wash assemblies

Remove the pump head and seal-wash assemblies whenever you need to:

• Replace the plunger seals and O-ring

• Clean or replace a plunger

Piston chamber components

3

2

8

1

9

7

6

4

5

10

11

12

3-4 Maintenance

Required materials

• Squeeze bottle with methanol

• Seal insertion/extraction tool

• 1/2-inch, 5/8-inch, and 5/16-inch open-end wrenches

• T27 TORX driver

To remove the pump head and seal-wash assemblies:

1. Flush the binary gradient module with an appropriate solvent.

2. In the MassLynx Main window, select Diagnostics > Manual Plunger Control.

Key

Number Name

1 Seal-wash housing

2 Transducer inlet tube assembly

3 Transducer assembly

4 Inlet check valve holder

5 Inlet check valve cartridge

6 Pump head

7 O-ring

8 Plunger seal

9 Plunger wash seal

10 Head support plate

11 Plunger retaining nut

12 Plunger


Controlling the plunger

3. Select the pump you plan to perform maintenance on (A or B).

4. Select the head you plan to perform maintenance on (left or right).

5. Click Move Plunger Back. The plunger moves back.

6. Disconnect the pressure transducer cable from the front bezel.

Disconnecting the cable and inlet check valve

7. Using the 1/2-inch open-end wrench, disconnect the inlet check valve fitting from the pump head. Keep the check valve cartridge inside the check valve fitting.

8. Using a 5/16-inch wrench, disconnect the transducer outlet tube connection at the manifold fitting.

3-6 Maintenance

Disconnecting the transducer outlet tube

9. Disconnect the two seal-wash tubes from the sides of the pump head.

10. While supporting the pump head’s weight, use a T27 TORX driver to remove the four screws in the support plate.

Removing the head assembly from the housing

11. Gently separate the pump head assembly from the housing.

12. Place the transducer face of the head assembly on a flat work surface.

Caution: Keep the pump head aligned with the plunger to avoid damaging the plunger. !


13. Use a T27 TORX driver to remove the two screws securing the pump head assembly to the support plate, and then remove the support plate.

Removing the pump head assembly

Caution: When you are placing the head assembly on a flat surface, take care not to damage the cable that protrudes from the transducer face. !

Remove the screws that secure the pump head assembly to the support plate.

Remove the support plate.

3-8 Maintenance

14. Remove the seal-wash housing.

Removing the seal-wash housing

15. In the Manual Plunger Control dialog box, click Move Plunger Forward. The plunger moves forward.


Replacing the seals and O-ring

This procedure involves replacing the:

• Plunger seal

• O-ring

• Plunger wash seal

Required materials

• Plastic tweezers (part number 605000106)

• Replacement seal kit (part number 700001494)

• Seal insertion/extraction tool (part number 405001667)

• Squeeze bottle with methanol (or an appropriate miscible solvent)

To replace the plunger seal:

1. Remove the head (see page 3-4).

2. Insert the seal insertion/extraction tool into the head and use a rocking motion to lift the seal from the head.

Removing the plunger seal

Caution: To avoid damaging the sealing surfaces, use the seal insertion/extraction tool. Do not use a sharp or metallic tool to remove or install seals. !

3-10 Maintenance

3. Remove the O-ring using plastic tweezers.

Removing the O-ring

4. Wet the seal cavity and new plunger seal with methanol.

Wetting the seal cavity and new plunger seal

5. Using the seal insertion/extraction tool, insert the new plunger seal into the pump head.

Installing the plunger seal


6. Insert the new O-ring into the O-ring groove in the pump head.

Installing the O-ring

7. Remove the seal-wash assembly as described on page 3-4.

8. Remove the existing plunger wash seal using plastic tweezers.

Removing the existing plunger wash seal

9. Wet the new plunger wash seal with methanol and insert it into the seal-wash housing.

Installing the new plunger wash seal

3-12 Maintenance

10. Insert the seal-wash assembly into the pump assembly and align the screw hole in both assemblies.

11. Reassemble the pump head, seal-wash assembly, and support plates and secure them with screws using the T27 TORX driver. Ensure that the drain fitting and check valve housing line up.

Securing the support plates

12. If you are replacing the plunger assembly, proceed to Replacing the plunger on page 3-16.

If you are not replacing the plunger assembly, proceed to Replacing an inlet check valve cartridge on page 3-20.

13. After you have replaced the seal, attach the head support plate, and then attach the seal-wash housing.

14. Use the T27 TORX driver to attach the two screws securing the pump head assembly to the support plate. Tighten the two screws 1/8 of a turn beyond finger-tight.

15. Wet both the seal cavity and the plunger before reinstalling the pump head.


Wetting the seal cavity

Wetting the plunger

16. Carefully align the pump head with the plunger and gently push the pump head onto the plunger, until the four screw holes align with the holes in the support plate.

17. Support the pump head’s weight and attach the four T27 TORX screws to the support plate. Tighten the four screws 1/8 of a turn beyond finger-tight.

18. Connect the two seal-wash tubes to the pump head.

Caution: To prevent damaging the plunger, ensure the plunger is retracted before performing the next step.!

3-14 Maintenance

Connecting seal-wash tubes

19. If the binary gradient module does not have solvent in the tubes, perform a wet prime to draw solvent into the plunger cavity.

Key

Number Name

1 Seal-wash A connection tube

2 Seal-wash A inlet tube

3 Seal-wash B outlet tube

4 Seal-wash B connection tube

5 Seal-wash B inlet tube

6 Seal-wash A outlet tube

2

6

4

3

5

1


Replacing the plunger

Required materials

• Grease pack

• Plunger assembly, 0.280-inch diameter (part number 700001532)

• Squeeze bottle with methanol


To replace the plunger:

1. In the MassLynx Main window, select Diagnostics.

2. Select Diagnostics > Manual Plunger Control.

3. In the Pump area, select the pump you plan to perform maintenance on.

4. In the Head area, select the head you plan to perform maintenance on.

5. Click Move Plunger Forward. The plunger moves forward.

6. Carefully slide a 5/8-inch open-end wrench over the plunger, and loosen the plunger-retaining nut. Remove the plunger.

Removing the plunger retaining nut

3-16 Maintenance

7. Using the grease pack supplied with the plunger, apply grease to the ball-end of the new plunger.

Greasing the plunger

8. Install the e-clip, flat washer, and three wave washers in that order. Make sure the wave washers are bowed toward the plunger tip.

Installing the e-clip and washers

Key

Number Name

1 E-clip

2 Plunger tip

3 Flat washer

3

4

1

2

5


9. Insert the new plunger into the actuator piston, and tighten the plunger-retaining nut with a 5/8-inch open-end wrench. Be careful not to damage the plunger when sliding the open-end wrench over it.

10. Align the new plunger so that it points straight out from the piston, to make it easier to install the pump head without damaging the plunger.

Reinstalling the pump head

To reinstall the pump head:

1. Lubricate the plunger seals (inside the pump head) with methanol just before reinstalling the pump head.

2. Orient the pump head so that the inlet check valve is on the bottom.

3. Carefully align the pump head with the plunger, and gently push the pump head onto the plunger, until the four screw holes align with the holes in the support plate.

4. While supporting the weight of the pump head, use the T27 TORX driver to tighten the four screws in the support plate 1/8 of a turn beyond finger-tight.

5. Reattach the two seal-wash tubes to their fittings at the sides of the pump head. Make sure the ferrules are in place.

6. Connect the solvent inlet tube to the inlet check valve on the bottom of the pump head.

7. Reconnect the transducer outlet tube to the manifold, using a 5/16-inch open-end wrench. The outlet is the top port on the side of the manifold.

4 Wave washers (3)

5 Plunger retaining nut

Caution: To avoid damaging the plunger, keep the pump head aligned with the plunger when performing the next step. Also, ensure the plunger is in the retracted state.

Key (Continued)

Number Name

!

3-18 Maintenance

8. Reconnect the transducer cable to the appropriate connector on the front panel, making sure it clicks into place.

9. Prime the binary gradient module.


Replacing an inlet check valve cartridge

The binary gradient module has one inlet check valve per pump head. The check valves are made of synthetic ruby and sapphire contained in easy-to-replace cartridges.

Required materials


• Replacement check valve cartridge (part number 700001493)


• 30-mL syringe (part number WAT271053, included in Startup Kit)


To replace an inlet check valve:

1. Remove the fitting at the inlet check valve holder.

2. Use the 1/2-inch wrench to remove the check valve holder from the head.

3. Invert the inlet valve cartridge holder to remove the old cartridge. Use plastic tweezers if the cartridge is stuck.

Removing the inlet check valve cartridge

4. Inspect the check valve holder and clean it if necessary. Rinse the components with methanol or an appropriate solvent.

5. Wet the new check valve cartridge with an appropriately miscible solvent.

3-20 Maintenance

Wetting the new inlet check valve cartridge

6. Insert the replacement cartridge into the holder with the arrow pointed away from the hex nut.

Inserting the inlet check valve cartridge

7. Insert the cartridge holder in the head and finger tighten the fitting.

8. Use the 1/2-inch wrench to tighten the check valve holder 1/8 of a turn beyond finger-tight.

9. Reattach the fitting from the solvent inlet tube to the check valve holder.

10. If the binary gradient module does not have solvent in the tubes, perform a dry prime before you perform a wet prime or start delivery of solvents (see page 2-18).

Replacing an inlet check valve cartridge 3-21

Replacing an outlet check valve cartridge

The binary gradient module path contains two outlet check valves per pump manifold. The check valves are made of synthetic ruby and sapphire in easy-to-replace cartridges.

Required materials


• Replacement check valve cartridge (part number 700001493)


• 30-mL syringe (part number WAT271053, included in Startup Kit)


To replace an outlet check valve:

1. Remove the fitting at the outlet check valve holder.

2. Use the 1/2-inch wrench to remove the check valve holder from the manifold.

Disconnecting the outlet check valve holder

3-22 Maintenance

3. Use plastic tweezers to remove the existing cartridge from inside the manifold.

Removing the existing outlet check valve cartridge

4. Inspect the check valve holder and clean it if necessary. Rinse the components with methanol or an appropriate solvent.

5. Wet the replacement check valve cartridge with an appropriately miscible solvent.

6. Insert the replacement cartridge into the holder with the arrow pointed upward.

7. Insert the cartridge holder in the manifold and finger tighten the fitting.

8. Use the 1/2-inch wrench to tighten the check valve holder 1/8 of a turn beyond finger-tight.

9. If the binary gradient module does not have solvent in the tubes, perform a dry prime to push solvent into the pump head (see page 2-18).

10. Perform a wet prime or start delivery of solvents.

Caution: To avoid damaging the check valve holder, always use plastic tweezers when performing the next step. !

Replacing an outlet check valve cartridge 3-23

Replacing the vent valve rotor-seal assembly

Required materials

• Seal insertion/extraction tool (part number 405001667)

• Vent valve rotor-seal (part number 700001483)


To replace the vent valve rotor-seal assembly:

1. Using the 5/8-inch open-end wrench, loosen the preload assembly at the front of the vent valve, and then remove it.

Removing the preload assembly

2. Remove the rotor-seal assembly from inside the valve using the seal insertion/extraction tool.

Tip: If the rotor is stuck inside the valve, it may be necessary to change the valve state several times.

Removing the rotor-seal assembly

3-24 Maintenance

3. Place the new rotor-seal into the valve, aligning the key with the slot in the actuator. The rotor orientation does not matter, as long as the key aligns with the slot.

4. Replace the preload assembly onto the front of the valve and finger tighten it until it stops turning. When the preload assembly stops turning, the rotor-seal pressure or preload is correct.

5. Prime the binary gradient module.

Installing or replacing the fusesThe binary gradient module is shipped with two IEC-type fuses installed. The startup kit includes two spare fuses.

Required materials

• Flat-blade screwdriver

• Fuse, 10 A, 250 Vac, 5 × 20 mm, slow-blow, IEC type (part number 330000102)

To install or replace the power supply fuses:

1. Ensure that the power switch is in the Off position, then remove the power cord from the rear panel.

Warning: To avoid electrical shock and possible injury, remove the power cord from the rear panel of the instrument before you perform this procedure.


Rear panel of the 2545 Binary Gradient Module

Key

Number Name

1 Power entry module

2 Fuse holder

3 Connector B

4 Connector A

5 Ethernet RJ45 port

5

4

3

2

1

3-26 Maintenance

2. Use a flat-blade screwdriver to remove the fuse holder, which is located below the power entry module.

Removing fuses

3. Replace both fuses with fuses of the appropriate type, and reinsert them into the holder.

4. Reinstall the fuse holder in its receptacle in the rear panel.

5. Connect the power cord to the power entry module.

Caution: For continued protection against fire hazard, replace fuses with the appropriate type and rating. !


Diagnostics and configuration

The MassLynx software provides a number of diagnostics, including the ability to test the chromatographic pump and the ability to identify which binary gradient module is currently communicating with the diagnostics interface.

Diagnostics menu options

Menu option Description

Manual Control Provides direct control to the pump components (valves, event switches, etc.).

Leak Test Performs the leak test on the pump heads.

Life Counters Displays life counters.

Manual Plunger Control Moves the plunger back and forward (for plunger and seal replacement).

Pressure Transducers Offsets Displays pressure transducers offsets.

Seal Wash Prime Starts/stops pulsing the seal-wash pump.

Wet Prime Sets pump prime parameters and primes the pump.

Reset Pump Resets the pump.

Configuration > System Information

Displays firmware release information.

3-28 Maintenance

Performing the leak test

The leak test performs a pressure ramp up, and then monitors the pressure decay in the binary gradient module to determine if the inlet check valves, tubes, fittings, or plunger seals are faulty. Perform the leak test whenever you:

• Suspect leakage in the inlet check valves or plunger seals

• Perform maintenance on the binary gradient module

• Replace fittings

Tip: This procedure does not test the optional leak sensor module.

To perform the leak test:

1. Make sure that all fittings on the binary gradient module are tight.

2. Perform a wet prime using methanol at 10 mL/min for at least 3 minutes.

• Use solvent A to prime pump A.

• Use solvent B to prime pump B.

3. In the MassLynx inlet status page, select the Additional Status tab, and then select the Chroma button.

4. In the Diagnostics Chromatographic Pump dialog box, select Diagnostics > Leak Test.

Starting the leak test

5. In the Test Configuration area, select a pump from the Select Pump list.

6. From the Select Head list, select a pump head.


7. In the Test Pressure text box, enter the pressure limit for which you will test the pump.

8. Click Start Leak Test. The status of the leak test appears in the Test Progress area. When the leak test is finished, the Test Results area displays the results.

Typical leak test results

Results: The test pass criteria is a decay rate of 300 psi/min for each head. This is a fairly stringent criterion based on the analytical flow rate capability of the binary gradient module.

The other data that is displayed is for information only. If the leak test fails the test criteria, messages in the Diagnosis area of the Test Results section can help diagnose a leak.

3-30 Maintenance

Resolving leak problems

To resolve leak problems:

1. Inspect all fittings, tubing, and the vent valve rotor.

2. If leaks cannot be resolved by checking fittings and tubing, inspect the check valves and pump seals.

3. If both the right and left heads of either pump A or B fail with about the same decay rate, the leak is likely in the areas that are common to both pump heads, such as:

• Tubing between the outlet check valve and vent valve

• Fittings between the outlet check valve and vent valve

• Vent valve rotor


3-32 Maintenance

4 Troubleshooting

Contents:

Topic Page

System troubleshooting 4-2

Alarm reset 4-4

Error messages 4-5

Troubleshooting hardware 4-8

4-1

System troubleshooting

Follow these basic steps to perform system troubleshooting:

1. Examine the system, checking the simple things first. Is something obvious causing the problem? For example, is an instrument unplugged or improperly connected?

2. Compare the current system operation with the way the system operated before the problem started. To help you identify normal operating conditions:

• Record a map of your LC system (tubes and power connections).

• Keep a daily log.

• Run test chromatograms regularly.

Recommendation: Keep track of system parameters and the results of your chromatography during normal operation. Troubleshooting is easier if you know the typical conditions when the system is operating correctly.

Example: If your system usually runs at nnn psi with a certain method, is the system pressure currently in the same range, or drastically higher (possibly caused by a clog) or lower (possibly caused by a leak)? Are pressure fluctuations in the same pressure range as during normal operation?

When your system is installed, and each time you develop a new method, record the system conditions during normal operation.

3. Identify in the following order the symptom that varies from normal system operation:

• System pressure (high, low, or erratic)

• Baseline (plumbing-related or detector-electronics-related)

• Changes in peak retention time


4-2 Troubleshooting

• Loss of peak resolution

• Abnormal peak shape (smaller than expected, broad, tailing, etc.)

• Incorrect qualitative and/or quantitative results

4. For each isolated symptom, identify a list of suspected causes.

5. Run the performance tests for each chromatographic instrument to quickly determine if a problem exists with a particular instrument.

6. Read the troubleshooting information in Troubleshooting hardware on page 4-8. This table is organized according to the parameters in step 3 and allows you to narrow the possible causes of a symptom and find suggested corrective actions.

If you determine that there is a problem related to another component, refer to the appropriate operator's guide.

When to call Waters Technical Service

Many problems with a binary gradient module can be easily corrected. However, if you cannot correct a condition, you can contact Waters Technical Service. If you are in the USA or Canada, contact Waters Technical Service (800 252-4752). If you are not in the USA or Canada, visit http://www.waters.com and click About Waters > Worldwide Offices, or phone your local Waters subsidiary or Waters’ corporate headquarters at 34 Maple Street, Milford, MA 01757, USA. When you call, have the following information available:

• Completed normal operation checklist for the method you are using

• Nature of the symptom

• Waters Binary Gradient Module serial number

• Flow rate

• Operating pressure

• Mobile phase(s)

• Detector settings

• Type and serial number of column(s)

• Sample type

• Control mode (MassLynx, FractionLynx, No interaction, or other)

• Software versions and serial numbers

System troubleshooting 4-3

Alarm reset

When an error occurs, the binary gradient module displays an alarm message and emits three short beeps.

To acknowledge an alarm, press the Stop Flow button. The binary gradient module displays the main screen or the next alarm message, or resets the pump. Alarms sometimes require resetting the pump. If the pump requires a reset, it resets when the last alarm is acknowledged. When MassLynx downloads a method or resets communications, the binary gradient module acknowledges all outstanding alarms and resets the pump, if required.

4-4 Troubleshooting

Error messages

Explanation of error message :

Error message Explanation

Battery Backed Memory HW Failure

The battery backed memory failed its checksum verification test. The likely cause is a dead battery.

Fan Fail HW Detected The fan is not working properly. If this message persists, call Waters Technical Service.

General Failure A nonspecific error. If this message persists, call Waters Technical Service.

Host Communications HW Failure

The pump turned off flow after 1 minute with no host communications.

Inter Pump Communications HW Failure

A successful communications link could not be established between the pump A and B electronics. If this message persists, call Waters Technical Service.

Leak Detect Input Active If the system has the optional leak detection module, this message is normal. If not, connect a jumper between pins 6 and 7 on connector B. If this message persists, call Waters Technical Service.

Pump Homing Error: Excessive Restriction

A transducer pressure exceeding 6900 kPa (69 bar, 1000 psi) was encountered during homing. Check the outlet tubes, vent valve, and other fluid-handling interfaces that could have a blockage. If this message persists, call Waters Technical Service.

Pump Homing Error: Travel Range Failure

The pump head could not travel the full stroke distance after it detected the home position. If this message persists, call Waters Technical Service.

Pump HW Over Pressure The electronic hardware detected that the over-pressure limit was exceeded.

Error messages 4-5

Pump Motor Driver HW Fault

The electronics detected an over-current condition on the pump motors. If this message persists, call Waters Technical Service.

Pump Motor Lost Synchronization

A requested pump motor movement failed its encode feedback check. This is usually caused by pressures exceeding 34,500 kPa (345 bar, 5000 psi) at flow rates over 100 mL/min.

Solvent Select Valve HW Failure

The electronics detected an over-current condition in the manifold circuitry. If this message persists, call Waters Technical Service.

Stop Flow Input Activated The pump was stopped through the Stop Flow input on the rear panel.

Stop Flow Key pressed The pump was stopped from the Stop Flow button on the instrument.

System Over Pressure The system pressure exceeded the method- defined high-pressure limit. This may be caused by:• a bad transducer or transducer connection• a stoppage in the system such as a clogged

injector, plugged column, bent tubing, etc.

System Under Pressure The system pressure was below the method defined low-pressure limit for over 30 seconds. Possible causes include a leak, loss of flow, check valve malfunction, or setting the low-pressure limit too high.

Explanation of error message (Continued):


4-6 Troubleshooting

Transducer Range Error The pump detected an excessive offset voltage that was out of specification. This is likely an electronics failure but could also result from a failure where the system pressure was not vented successfully on power-up. If this message persists, call Waters Technical Service.

Vent Valve Fail HW Detected

The system/vent valve detected a movement error. If this message persists, call Waters Technical Service.

Explanation of error message (Continued):


Error messages 4-7

Troubleshooting hardware

This table contains suggestions for resolving hardware problems in the binary gradient module. If the suggested solutions do not solve the problem, call Waters Technical Service.

Troubleshooting the hardware :

Symptom Possible Cause Corrective Action

Display is blank Unit not powered on Power on unit.

Startup diagnostics failure

Power off and on again. If failure persists, call Waters Technical Service.

Contrast set too dark Press contrast button.

Erratic flow rate or pressures

Air bubble in pump head

Prime the binary gradient module.

Dirty inlet check valves

Clean/replace inlet check valves.

Prime/vent valve open or leaking

Close or rebuild prime/vent valve.

Plugged solvent reservoir filter

Clean or replace filter.

Plunger seal leaking Replace plunger seal assembly.

Front panel software “lock up”

Software problem or power line problem


Manifold leaking Defective solvent select valve

Call Waters Technical Service.

4-8 Troubleshooting

Priming problems; Will not start flow

Solvent connect tubes may be leaking

Check solvent connect tubes.Perform the leak test.

Pump heads not primed

Prime pump heads.

Solvent tube may be defective

Switch to opposite solvent tube (if you are using tube A1, switch to tube A2).

Defective check valve Replace the check valve cartridge.

Startup diagnostics fail Internal problem with controller board, solvent management system, or sample management system


Unit does not power on Power cord not connected

Check power cord.

No power at outlet Check line voltage.

Power supply fuse blown or missing

Replace power supply fuses.

Troubleshooting the hardware (Continued):

Symptom Possible Cause Corrective Action

Troubleshooting hardware 4-9

4-10 Troubleshooting

A Safety Advisories

Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This appendix presents all the safety symbols and statements that apply to the entire line of Waters products.

Contents

Topic Page

Warning symbols A-2

Caution symbol A-6

Warnings that apply to all Waters instruments A-7

Electrical and handling symbols A-14

A-1

Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

Task-specific hazard warningsThe following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others.

When the following symbols appear in a manual’s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of needle puncture.)

Warning: (Risk of injury caused by moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

A-2 Safety Advisories

Warnings that apply to particular instruments, instrument components, and sample types

The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts.

Burst warning

This warning applies to Waters instruments fitted with nonmetallic tubing.

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)

Warning: Pressurized nonmetallic, or polymer, tubing can burst. Observe these precautions when working around such tubing:• Wear eye protection.• Extinguish all nearby flames.• Do not use tubing that is, or has been, stressed or kinked.• Do not expose nonmetallic tubing to incompatible compounds like

tetrahydrofuran (THF) and nitric or sulfuric acids.• Be aware that some compounds, like methylene chloride and

dimethyl sulfoxide, can cause nonmetallic tubing to swell, which significantly reduces the pressure at which the tubing can rupture.

Warning symbols A-3

Mass spectrometer flammable solvents warning

This warning applies to instruments operated with flammable solvents.

Mass spectrometer shock hazard

This warning applies to all Waters mass spectrometers.

This warning applies to certain instruments when they are in Operate mode.

Warning: Where significant quantities of flammable solvents are involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 400 kPa (4 bar, 58 psi) during an analysis in which flammable solvents are used. Also ensure a gas-fail connection is connected to the HPLC system so that the LC solvent flow stops if the nitrogen supply fails.

Warning: To avoid electric shock, do not remove the mass spectrometer’s protective panels. The components they cover are not user-serviceable.

Warning: High voltages can be present at certain external surfaces of the mass spectrometer when the instrument is in Operate mode. To avoid non-lethal electric shock, make sure the instrument is in Standby mode before touching areas marked with this high voltage warning symbol.


Biohazard warning

This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

Chemical hazard warning

This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material.

Warning: Waters’ instruments and software can be used to analyze or process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials. To avoid infection with these agents, assume that all biological fluids are infectious, observe good laboratory practices, and consult your organization’s biohazard safety representative regarding their proper use and handling. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).

Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety representative regarding proper use and handling. Guidelines are provided in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals.

Warning symbols A-5

Caution symbol

The caution symbol signifies that an instrument’s use or misuse can damage the instrument or compromise a sample’s integrity. The following symbol and its associated statement are typical of the kind that alert you to the risk of damaging the instrument or sample.

Caution: To prevent static charges and resultant circuit damage, do not remove the covers that protect integrated circuit chips.


Warnings that apply to all Waters instruments

When operating this device, follow standard quality control procedures and the the equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktion-stüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: eventuali modifiche o alterazioni apportate a questa unità e non espressamente approvate da un ente responsabile per la conformità annulleranno l’autorità dell’utente ad operare l’apparecchiatura.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.


Warning: Use caution when working with any polymer tubing under pressure:• Always wear eye protection when near pressurized polymer tubing.• Extinguish all nearby flames.• Do not use tubing that has been severely stressed or kinked.• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated

nitric or sulfuric acids.• Be aware that methylene chloride and dimethyl sulfoxide cause

nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing.

Attention: Manipulez les tubes en polymère sous pression avec precaution:• Portez systématiquement des lunettes de protection lorsque vous vous

trouvez à proximité de tubes en polymère pressurisés.• Eteignez toute flamme se trouvant à proximité de l’instrument.• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane

(THF) ou de l'acide sulfurique ou nitrique concentré.• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le

gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht:• In der Nähe von unter Druck stehenden Polymerschläuchen stets

Schutzbrille tragen.• Alle offenen Flammen in der Nähe löschen.• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht

sind.• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder

konzentrierte Salpeter- oder Schwefelsäure verwenden.• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische

Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.


Attenzione: prestare attenzione durante l’utilizzo dei tubi di polimero pressurizzati:• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero

pressurizzati.• Estinguere ogni fonte di ignizione circostante.• Non utilizzare tubi soggetti che hanno subito sollecitazioni eccessive o son

stati incurvati.• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido

solforico o nitrico concentrato.• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano

rigonfiamento nei tubi non metallici, riducendo notevolmente la resistenza alla rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión:• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de

tubos de polímero sometidos a presión.• Si hubiera alguna llama las proximidades.• No se debe trabajar con tubos que se hayan doblado o sometido a altas

presiones.• Es necesario utilizar tubos de metal cuando se trabaje con

tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados.• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de

dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.



Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsach-gemäßer Verwenddung des Gerätes unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: l’utente deve essere al corrente del fatto che, se l’apparecchia-tura viene usta in un modo specificato dal produttore, la protezione fornita dall’apparecchiatura potrà essere invalidata.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.


Warning: To protect against fire hazard, replace fuses with those of the same type and rating.

Attention: Remplacez toujours les fusibles par d’autres du même type et de la même puissance afin d’éviter tout risque d’incendie.

Vorsicht: Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen.

Attenzione: per una buona protezione contro i rischi di incendio, sostituire i fusibili con altri dello stesso tipo e amperaggio.

Advertencia: sustituya los fusibles por otros del mismo tipo y características para evitar el riesgo de incendio.


Warning: To avoid possible electrical shock, disconnect the power cord before servicing the instrument.

Attention: Afin d’éviter toute possibilité de commotion électrique, débranchez le cordon d’alimentation de la prise avant d’effectuer la mainte-nance de l’instrument.

Vorsicht: Zur Vermeidung von Stromschlägen sollte das Gerät vor der Wartung vom Netz getrennt werden.

Attenzione: per evitare il rischio di scossa elettrica, scollegare il cavo di alimentazione prima di svolgere la manutenzione dello strumento.

Precaución: para evitar descargas eléctricas, desenchufe el cable de alimen-tación del instrumento antes de realizar cualquier reparación.


Electrical and handling symbols

Electrical symbolsThese can appear in instrument user manuals and on the instrument’s front or rear panels.

Electrical power on

Electrical power off

Standby

Direct current

Alternating current

Protective conductor terminal

Frame, or chassis, terminal

Fuse

Recycle symbol: Do not dispose in municipal waste.


Handling symbolsThese handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments.

Keep upright!

Keep dry!

Fragile!

Use no hooks!

Electrical and handling symbols A-15


B Specifications

Contents:

Topic Page

Physical specifications B-2

Environmental specifications B-3

Electrical specifications B-4

Solvent management system specifications B-7

B-1

Physical specifications

Physical specifications

Item Specification

Height 17 inches (43 cm)

Depth (without cables connected at rear; includes heads and tubes at front)

25 inches without drip tray (63.5 cm)

Width 11 inches (28 cm)

Weight 125 pounds (57 kg)

B-2 Specifications

Environmental specifications

Environmental specifications

Item Specification

Operating temperature 4 to 40 °C

Humidity 20 to 80%, noncondensing

Acoustic noise (instrument-generated)

<60 dBA at idle

Solvent compatibility Solvents consistent with materials of construction. Salts and buffers can reduce seal life, especially at pressures in excess of 20,700 kPa (207 bar, 3000 psi).

Internal cooling DC-powered fans provide internal cooling by pulling air through the binary gradient module and exhausting it out the rear of the unit.

Airflow requirements Air enters the front of the binary gradient module so pumps can be placed side by side.

Clearance Allow at least 6 inches (15 cm) between the rear of the binary gradient module and the wall for power connections and the ventilation fans’ exhaust.

Environmental specifications B-3

Electrical specifications

Electrical specifications

Item Specification

Protection classa Class I

Overvoltage categoryb II

Pollution degreec 2

Moisture protectiond Normal (IPXO)

Line voltages, nominal

Grounded AC

Current requirements 6.5 A

Line voltage 100 to 240 Vac ±10%

Frequency 50/60 Hz

Fuse 10 A, 5 × 20 mm, slow-blow, IEC type

Power consumption 650 W

Time or user-controllable switch closures S1 to S4

Four controllable contact closures (two terminals per closure).Maximum allowable current = 0.5 A per contact. Maximum allowable voltage = 30 Vdc.Contact resistance = 0.2 ohms. Outputs can be controlled from the I/O Events table or directly from the front panel.Two modes available from front panel and I/O Events table:On = contact closed. Off = contact open.Additional modes in the I/O Events table: Pulse = single contact closure for programmable period.Toggle = changes the current state.

B-4 Specifications

Stop flow (input) TTL signal or contact closure.Input voltage range: ±30 Vdc.Logic high = >3.0 Vdc ±10%. Logic low = <1.9 Vdc ±10%. Minimum pulse width = 100 msec. Screw terminal connector.

Start gradient (input) TTL signal or contact closure.Input voltage range: ±30 Vdc. Logic high = >3.0 Vdc ±10%.Logic low = <1.9 Vdc ±10%.Minimum pulse width = 100 msec.Screw terminal connector.

Chart output (1) Two terminals (+, –) for recording the following user-selectable outputs:Programmed flow rate (2 V output = 150 mL/min). System pressure (2 V output = 6000 psi).Programmed composition (%A, %B)(2 V output = 100% composition).

Chart output (2) Two terminals (+, –) for recording the following user-selectable outputs:Programmed flow rate (2 V output = 150 mL/min). System pressure (2 V output = 6000 psi).Programmed composition (%A, %B)(2 V output = 100% composition).

Leak detector input TTL signal or contact closure.Input voltage range: ±30 Vdc. Logic high = >3.0 Vdc ±10%.Logic low = <1.9 Vdc ±10%.Minimum pulse width = 100 msec.Screw terminal connector.

Electrical specifications (Continued)

Item Specification

Electrical specifications B-5

Auxiliary input 2 TTL signal or contact closure.Input voltage range: ±30 Vdc. Logic high = >3.0 Vdc ±10%.Logic low = <1.9 Vdc ±10%.Minimum pulse width = 100 msec.Screw terminal connector.

Ground terminals Connected to signal ground and used as reference for outputs.

a. Protection Class I – The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system. In turn, this grounding system is connected to the third pin (ground pin) on the electrical power cord plug.

b. Overvoltage Category II – Pertains to instruments that receive their electrical power from a local level such as an electrical wall outlet.

c. Pollution Degree 2 – A measure of pollution on electrical circuits, which may produce a reduction of dielectric strength or surface resistivity. Degree 2 refers only to normally nonconductive pollution. Occasionally, however, expect a temporary conductivity caused by condensation.

d. Moisture Protection – Normal (IPXO) – IPXO means that no Ingress Protection against any type of dripping or sprayed water exists. The X is a placeholder that identifies protection against dust, if applicable.

Electrical specifications (Continued)

Item Specification

B-6 Specifications

Solvent management system specifications

Solvent management system specifications

Item Specification

General High pressure mixing, binary gradient.

Priming Ability to self-prime from a solvent container on the floor with wet pump heads and dry tubes (no intervention from the operator such as a syringe).

Number of solvents Up to four (4): A1, A2, B1, and B2. User must choose A1 or A2 and B1 or B2 before the start of the gradient.

Solvent and seal-wash containers

User-selectable.

Primary wetted materials 316 stainless steel, UHMWPE, Sapphire, Ruby, Tefzel (ETFE), Teflon (FEP, PTFE, PFA), Nitronic 60, Valcon H, Chemraz, and PEEK™.

Pump seal-wash (for two seal-wash pumps)

Pump and seals required to wash the rear of the high-pressure seal (pulsed type flow, about 20 µL per pulse).

Flow rate, pressure operating envelope

See page B-9.

Flow rate increment 0.01 mL/min.

Delay volume Delay volume of the binary gradient module is effectively zero because the two pumps are connected together at the outlet filter/tee. System delay volume depends on the tubes and/or mixers and fittings between the outlet of the binary gradient module and the column inlet.

Flow accuracy ±1.0% of required flow or 10 mL/min, whichever is greater. Flow rate 1.00 to 150.00 mL/min, using water or MeOH, back pressure approximately 4140 kPa (41 bar, 600 psi).


Composition accuracy ±1.0% absolute (full scale) from 5 to 95% from 1.0 to 130 mL/min using water/water or MeOH/MeOH spiked with dye or marker, back pressure approximately 4140 kPa (41 bar, 600 psi).

Composition precision Retention time variation less than 0.3% RSD or 0.1 min SD, whichever is greater. Standard tests at 1.00, 20.00, 50.00 mL/min, test mix Uracil/Caffeine, Xterra columns 4.6 × 50, 19 × 50, 30 × 50, injection volumes 6, 100, 500 µL. Ambient temperature fluctuations may affect results.

Gradient composition curves

Eleven gradient curves (including linear, step [2], concave [4], and convex [4]).

Solvent management system specifications (Continued)

Item Specification

B-8 Specifications

Flow envelope

Instrument control and communication specifications

Item Specification

Ethernet Control from external PC.

RS-232 Reserved for future use.

Pressure (psi)

6000

5000

100 150

Flow rate (mL/min)


B-10 Specifications

Index

Numerics2545 Binary Gradient Module. See

Binary Gradient Module

Aaccessories, unpacking 1-6acoustic noise 1-4airflow requirements 1-4alarm message 4-4alarm, reset 4-4analog data, entering 2-16auxiliary 2 terminals 1-18

BBinary Gradient Module

configuring 2-1overview 1-2powering on 2-2unpacking 1-6

biohazard warning A-5burst warning A-3

Ccalibration guidelines 1-ivcaution symbol A-6check valve cartridges, replacing 3-22chemical hazard warning A-5clearance requirements 1-4communication specifications B-9configuring Binary Gradient Module

2-1contacting Waters Technical Service

4-3current requirements, fusing 1-5

Ddepth requirement 1-4drip tray, installing 1-14

dry prime, performing 2-18

Eelectrical specifications B-4electrical symbols A-14entering analog information 2-16event switches 1-18

Fflammable solvents A-4flow envelope B-9frequency requirement 1-5fuses

installing 3-25replacing 3-25

Ggradient start 1-17ground terminals 1-17guidelines, calibration 1-iv

Hhandling symbols A-15hardware troubleshooting 4-8height requirement 1-4humidity requirement 1-4

II/O event switches 1-18inlet method, creating 2-8installing

drip tray 1-14fuses 3-25seal-wash waste tube 1-13system outlet tube 1-15vent valve waste tube 1-14

instrument control specifications B-9

Index-1

intended use 1-ivinternal cooling requirement 1-4

Lleak detector signals 1-18leak problems, resolving 3-31leak test, performing 3-29line voltage requirement 1-4

Mmaintenance

safety and handling 3-2mass spectrometer shock hazard A-4

Ooperating procedures, safety 3-2operating temperature requirement

1-4O-ring, removing 3-11outlet check valve cartridges, replacing

3-22overview, Binary Gradient Module 1-2

Pphysical specifications B-2plunger seal, removing 3-10plunger, reinstalling pump head 3-18plunger-seal-wash pump, priming 2-17power consumption requirement 1-5power supply fuse 3-25powering on, Binary Gradient Module

2-2priming

Binary Gradient Module 2-19dry prime 2-18plunger-seal-wash pump 2-17

pump headreinstalling 3-18removing 3-4

Rremoving

O-ring 3-11plunger seal 3-10pump head 3-4seal-wash assembly 3-4

replacingfuses 3-25outlet check valve cartridges 3-22pump plunger 3-16rotor-seal assembly 3-24

requirementsairflow 1-4clearance 1-4depth 1-4frequency 1-5fusing current 1-5height 1-4humidity 1-4internal cooling 1-4line voltage 1-4power consumption 1-5weight 1-4width 1-4

resolving leak problems 3-31

Ssafety advisories A-1seal life 3-3seals, replacing 3-10seal-wash assembly

removing 3-4replacing seals 3-12

seal-wash waste tube 1-13serial number, location 1-6signal connections

I/O 1-16signals, leak detector 1-18solvent reservoirs 2-17

Index-2

specificationselectrical B-4environmental B-3instrument control and

communication B-9physical B-2solvent management system B-7

start gradient, terminals 1-17startup kit contents 1-6stop flow, terminals 1-18support plates, securing 3-13switch states 2-14switch terminals 1-17symbols

caution A-6electrical A-14handling A-15warning A-2

system outlet tube 1-15system troubleshooting 4-2

Ttemperature requirement 1-4terminals

auxiliary 2 1-18ground 1-17start gradient 1-17stop flow 1-18switch 1-17

timed events, entering information 2-14

trademarks 1-iitroubleshooting

error messages 4-5hardware 4-8system 4-2

tubesseal-wash waste 1-13vent valve waste 1-14

I

Uunpacking the Binary Gradient Module

1-6

Vvent valve rotor-seal assembly,

replacing 3-24vent valve waste tube 1-14voltage, line requirement 1-4

Wwarning symbols A-2, A-7waste tube

seal-wash 1-13vent valve 1-14

Waters Technical Service, contacting 4-3

weight requirement 1-4width requirement 1-4

Index-3

Index-4

Documents

2545 Binary Gradient Module Operator’s Guide · 2007-11-21 · Gradient Module, or the 3100 Mass Detector) supports no more than two vertically oriented “accessory” Waters modules