2016.ASHI-HLA...covering HLA haploidentical transplant and permissible mismatches. 4. Novel treatment approaches of allograft rejection targeting B cells, plasma cells, and complement

FINAL PROGRAM2016.ASHI-HLA.ORG

ASHI • 2016 FINAL PROGRAM BOOK | 1

Bio-Rad Laboratories

CareDx, Inc.

Elsevier

GenDx

Histogenetics

Illumina, Inc.

Immucor

Linkage Biosciences Inc.

MLC Group, LLC

National Marrow Donor Program

Omixon

One Lambda Inc, a Thermo Fisher Scientific Brand

STEMCELL Technologies Inc.

USB Products by Affymetrix

THANK YOU TO THE 2016 ASHI CORPORATE SUPPORTERS

2 | ASHI • 2016 FINAL PROGRAM BOOK

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Program Planning Committee . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Abstract Reviewers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Board of Directors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Corporate Supporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

Exhibitor Directory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

Award Winners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Schedule at a Glance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Floorplans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

TABLE OF CONTENTS


GENERAL INFORMATIONREGISTRATION/CYBER CAFE/ DR. PAUL I. TERASAKI TRIBUTERegistration is located on the fourth level of the Hyatt Regency St. Louis at the Arch in the Grand Ballroom Prefunction. Dr. Paul I. Terasaki Tribute will be located in Sterling I on the second level.

Monday, September 26 7:00 am – 8:00 pmTuesday, September 27 7:00 am – 5:30 pmWednesday, September 28 7:30 am – 5:30 pmThursday, September 29 8:00 am – 6:00 pmFriday, September 30 7:30 am – 12:00 pm

SPEAKER READY ROOMThe Speaker Ready Room is located in Sterling 4 on the second level of the Hyatt Regency St. Louis at the Arch.

Monday, September 26 7:00 am – 7:30 pmTuesday, September 27 7:00 am – 5:00 pmWednesday, September 28 7:30 am – 6:00 pmThursday, September 29 8:00 am – 5:30 pmFriday, September 30 7:30 am – 10:30 am

EXHIBITSExhibits are located in the Grand Ballroom of the Hyatt Regency St. Louis at the Arch.

Exhibit & Poster Viewing HoursMonday, September 26 8:00 pm – 9:00 pmTuesday, September 27 9:00 am – 11:30 am 1:30 pm – 6:30 pmWednesday, September 28 10:00 am – 12:30 pm 2:30 pm – 6:00 pmThursday, September 29 10:00 am – 12:30 pm

Poster Mounting TimesMonday, September 26 12:00 pm – 7:00 pm*

Poster Dismounting TimesThursday, September 29 7:00 pm – 8:00 pm**

* All posters should be mounted by 7:00 pm on Monday, September 26. If additional time is needed, please visit the registration desk. ** Any poster still in place at 8:00 pm on Thursday, September 29, will be discarded.


CYBER CAFÉThe cyber café is located in the Grand Ballroom Prefunction. Complimentary computer stations are provided enabling you to access the internet for all your meeting needs while at the 42nd Annual Meeting. Use of the computers is limited to 15 minutes per session.

ADA COMPLIANCEASHI fully complies with the legal requirements of the Americans with Disabilities Act. If any participant is in need of special accommodations, please notify the hotel and indicate the type of assistance needed. ASHI cannot ensure the availability of appropriate assistance without advance notice.

CAMERAS AND CELL PHONESRecording or taking photographs during ASHI educational programming is prohibited. Any violation of this policy may result in the offender being removed from the meeting. As a courtesy to fellow attendees, please turn off cell phones during educational sessions.

LEARNING OBJECTIVES The ASHI 42nd Annual Meeting will provide updates on basic immunogenetics and on practice guidelines in clinical histocompatibility and immunogenetics.

The learning objectives are as follows:1. HLA ligand specificity in lymphocyte response, and description of

functional elements within HLA genes and molecules.2. Application of next generation sequencing covering technology

implementation, data standardization, utility in clinical transplantation, and the role of HLA in disease association.

3. Application of HLA genetics to hematopoietic stem cell transplantation covering HLA haploidentical transplant and permissible mismatches.

4. Novel treatment approaches of allograft rejection targeting B cells, plasma cells, and complement.

5. Optimization of clinical outcomes in lung transplantation covering donor allocation and HLA compatibility.

6. Update on the effect of red blood cell, platelet, and neutrophil antibodies in transplantation.

7. Update on the new Kidney Allocation System covering its effect on donor allocation, identification of HLA antibody specificity, HLA laboratory practices, and graft survival.

8. Updates on clinical practice and regulatory guidelines for directors and technologists.

GENERAL INFORMATION


EVALUATIONSParticipants must complete an evaluation in order to receive a certificate documenting credits earned for attending sessions. Sessions must be attended in their entirety. Partial credit is not available. Following the meeting, complete the evaluation and print your certificate by visiting 2016.ashi-hla.org and clicking on the evaluation specific icon. A username and password will be provided to you via email upon the end of the meeting. Online meeting evaluations will be available from September 26 – December 23, 2016. Certificates will no longer be available after December 23rd.

ACCREDITATION STATEMENTThis activity has been planned and implemented in accordance with the accreditation requirements and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the jointprovidership of Amedco and the American Society of Histocompatibility and Immunogenetics (ASHI). Amedco is accredited by the ACCME to provide continuing medical education for physicians.

AMA PRA STATEMENTAmedco designates this live activity for a maximum of 34.75 AMA PRA Category 1 CreditsTM. Physicians should claim only the credit commensurate with the extent of their participation in the activity.

CHT, CHS, ABHI DIPLOMATESThe American Board of Histocompatibility & Immunogenetics has approved the 42nd Annual Meeting content for a maximum of 34.5 contact hours (4 contact hours for Inspectors’ Workshop and 30.5 contact hours for the meeting) or 5.175 CECs (0.6 CECs for Inspector’s Workshop and 4.575 CECs for the meeting) of continuing education hours for completing each module.

ABSTRACT AWARDSThe following awards will be presented to the highest ranked abstracts accepted for oral presentations: ASHI Scholars, International Scholar, Best Solid Organ Case Study and Best Stem Cell Case Study. Four posters will be awarded for the following: Most Innovative, Most Clinically Relevant, President’s Choice, and People’s Choice.

GENERAL INFORMATION


PROGRAM PLANNING COMMITTEE

PROGRAM PLANNING CHAIRMalek Kamoun, MD, PhDUniversity of Pennsylvania Philadelphia, PA

PLENARY AND SYMPOSIUM SESSIONSSharon Adams, MT(ASCP), CHS(ABHI)National Institutes of HealthBethesda, MD

Medhat Askar, MD, PhD, D(ABHI)Baylor University Medical CenterDallas, TX

Marcelo Fernandez-Vina, PhD, D(ABHI)Stanford University School of MedicinePalo Alto, CA

Howard Gebel, PhD, D(ABHI)Emory University Hospital Atlanta, GA

Willie Hildebrand, PhD, D(ABHI)University of Oklahoma Health Science CenterOklahoma, OK

Toni Lyrenmann, BS, CHS, CLSUniversity of MinnesotaMinneapolis, MN

Martin Maiers, PhDNational Marrow Donor Program Minneapolis, MN

Elaine Reed, PhD, D(ABHI)UCLA Immunogenetics CenterLos Angeles, CA

Anat Tambur, DMD, PhD, D(ABHI)Northwestern UniversityChicago, IL

ABSTRACT CHAIRS

Patricia Campbell, MBChB, FRCPUniversity of AlbertaEdmonton, AB

Dimitri Monos, PhDUniversity of PennsylvaniaPhiladelphia, PA

Rajalingam Raja, PhD, D(ABHI)University of California, San FranciscoSan Francisco, CA

George Vlad, PhDColumbia UniversityNew York, NY

BASIC SCIENCE IMMUNOGENETICS CHAIR

Peter Parham, PhDStanford University School of MedicinePalo Alto, CA

WORKSHOP CHAIRS

Brett Loehmann, CHT, CHS, CTBS, BSMid-South Transplant HLA LaboratoryCordova, TN

Donna Phelan, BA, CHS, MT(HEW)Barnes-Jewish Hospital St. Louis, MO

CONTINUING MEDICAL EDUCATION ACCREDITATION MANAGER

Sheryl MorganAmedco


Margareth Afonso Torres, MT (ASCP)Hospital Israelita Albert Einstein

Moheeb Al-Awwami, Ph.D, CHT, CHSKing Faisal Hospital Specialist Hospital & Research Center

Balamurugan ArumugamUCLA School of Medicine

Fleur Aung, MDMD Anderson Cancer Center

Jorge Baluarte, MDThe Children’s Hospital of Philadelphia

Lee Ann Baxter-Lowe, PhD, D(ABHI)Childrens Hospital of Los Angeles

Noureddine Berka, PhD, D(ABHI)Calgary Laboratory Services

Maria Bettinotti, PhD, D(ABHI), FACMGJohns Hopkins University School of Medicine

Rainer Blasczyk, MD, PhDHannover Medical School

Nancy Bunin, MDThe Children’s Hospital of Philadelphia

Kevin BurnsBloodSource

Patricia Campbell, MBChB, FRCPUniversity of Alberta Hospitals

Manuel Carreno, MDOlerup, Inc.

Michael J. Cecka, PhD, D(ABHI)University of California Los Angeles

Dominique Charron, PhDHôpital Saint Louis

Rodica Ciubotariu, MDNew York Blood Center

Frans Claas, PhDLeiden University Medical Center

Beth Colombe, PhD, D(ABHI)Thomas Jefferson University Hospital

Marco Colonna, MDWashington University School of Medicine

Daniel Cook, PhDOne Lambda, Inc.

Deborah Crowe, PhD, D(ABHI)DCI Laboratory

Steven DeGoey, BS, CHSMayo Clinic

Julio Delgado, MD, MS, D(ABHI)University of Utah

Sara Dionne, PhDLabs, Inc.

Ronald Domen, MDPenn State Milton S. Hershey Medical Center Cindy Ellison

Cindy Ellison, PhD, D(ABHI) University of Manitoba

Mohamed Elrefaei, MD, PhDMayo Clinic in Florida

Hooi Sian Eng, PhDUniversity Malaya

ABSTRACT REVIEWERS


Sallyanne Fossey, PhD, D(ABHI)DCI Transplant Immunology Laboratory

Sujata Gaitonde, MDUniversity of Illinois At Chicago

Manish Gandhi, MDMayo Clinic

Xiaojiang Gao, PhDLeidos Biomedical Research, Inc/NCI

Howard Gebel, PhD, D(ABHI)Emory University Hospital

Ketevan Gendzekhadze, PhDCity of Hope

Maria Gerbase-De Lima, MD, PhDFederal University of San Paulo

Alin Girnita, MD, D(ABHI)University of Cincinnati Academic Health Center

David Gjertson, PhDUCLA Immunogenetics Center

Clara Gorodezky, PhD, DScFundacion Comparte Vida, A.C.

Lisbeth Guethlein, PhDStanford University

Amy Hahn, PhD, D(ABHI)Albany Medical College

Gregory Hale, MDAll Children’s Hospital

Anne Halpin, MScUniversity of Alberta Hospital

Vera Hauptfeld, PhD, D(ABHI)University of Alabama at Birmingham

Michelle Hickey, MD, PhdUniversity of California, Los Angeles

Luis Hidalgo, PhD, D(ABHI)University Alberta Hospitals

Nancy Higgins, MT, CHSIndiana University Health Methodist

Chak-Sum Ho, PhDGift of Life Michigan

Susan Hsu, PhDAmerican Red Cross Blood Services

Charlene Hubbell, BS, MT(ASCP), SBBSUNY Upstate Medical University

Annette Jackson, PhDJohns Hopkins University School of Medicine

Malek Kamoun, MD, PhDUniversity of Pennsylvania

Sivadasan Kanangat, PhD, D(ABHI)Rush University Medical Center

Ronald Kerman, PhDBaylor College of Medicine

Faisal Khan, PhDUniversity of Calgary

Ellen Klohe, PhD, D(ABHI)Inland Northwest Blood Center

Janette Kwok, PhDQueen Mary Hospital

ABSTRACT REVIEWERS


Peter Lalli, PhDCarolinas Health System

Sue Leffell, D. ABMLI, D(ABHI)Johns Hopkins University School of Medicine

Robert Liwski, MDDalhousie University

Andrew Lobashevsky, MD, PhD, D(ABHI)Indiana University School of Medicine

Mayra Lopez-Cepero, PhD, D(ABHI)LifeLink Foundation

Steven Mack, BA, PhDChildrens Hospital Oakland

Gabriel Maine, PhDWilliam Beaumont Hospital

Susana Marino, MD, PhD, D(ABHI)University of Chicago Hospital Medical Center

James M. Mathew, PhDNorthwestern University, Feinberg School of Medicine

Neema Mayor, PhDAnthony Nolan

Diogo MeyerInstituto de Biociências da USP

Derek Middleton, DSc, PhD, FRCPathRoyal Liverpool University Hospital

Joshua Miller, MDNorthwestern University Feinberg School of Medicine

Thalachallour Mohanakumar, PhD, D(ABHI)St. Joseph’s Hospital and Medical Center

Dimitri Monos, PhDUniv of Pennsylvania/ Children’s Hospital of Philadelphia

Gerald Morris, MD, PhDUniversity of California, San Diego

Omar Moussa, PhDFairview Medical Center, University of Minnesota, Minneapolis

Cathi Murphey, PhDSouthwest Immunodiagnostics, Inc.

Karen Nelson, PhD, D(ABHI)Bloodworks Northwest

Harriet Noreen, BS, CHSFairview University Medical Center

Allen Norin, PhD, D(ABHI)SUNY Downstate Medical Center

Carol Pancoska, PhD, D(ABHI)Einstein Medical Center

Marcelo J. Pando Rigal, PhD, D(ABHI)Mayo Clinic Arizona

Donna Phelan, BA, CHS, MT(HEW)Barnes-Jewish Hospital

Jeffrey PlattUniversity of Michigan

Michele Prod, BS, MT(ASCP), CHSRush University Medical Center

Rajalingam Raja, PhD, D(ABHI)University of California, San Francisco

ABSTRACT REVIEWERS


Daniel Ramon, PhD, D(ABHI), HCLDUniversity of Michigan Medical School

Bryan Ray, PhDGen-Probe Transplant Diagnostics

Sandra Resto-Ruiz, PhD, HCLD(ABB)LifeLink Transplant Immunology Laboratory

Lucie RichardHema-Quebec

Joseph Rossano, MD, MS, FAAP, FAACThe Children’s Hospital of Philadelphia

Susan Saidman, PhD, D(ABHI)Massachusetts General Hospital

Jennifer Schiller, PhD, D(ABHI)BloodCenter of Wisconsin

David Senitzer, PhD, D(ABHI ABMLI)City of Hope National Cancer Center

Alessandro Sette, PhDLa Jolla Institute For Allergy and Immunology

Hiroko Shike, MD, D(ABHI)Penn State Hershey Medical Center

Sadeep Shrestha, PhD, MHS, MSUniversity of Alabama at Birmingham

Ruby Siegel, MS, CHS (ABHI)Stanford Histocompatibility, Immunogenetics & Disease Profiling Laboratory

Anat Tambur, DMD, PhD, D(ABHI)Northwestern University

Rasmi Thomas, PhDMilitary HIV Research Program

Victoria Turner, PhD, D(ABHI)Laboratory Consultants of Florida

Dolly Tyan, PhD, D(ABHI)Stanford University

George Vlad, PhDColumbia University

William Ward, PhD, D(ABHI)National Institutes of Health

Qingyong Xu, PhDLondon Health Sciences Centre – University Hospital

Danny Youngs, BS, CHSBloodworks Northwest

Andrea Zachary, PhD, D(ABHI)Johns Hopkins University School of Medicine

Iwen Zhang, MD, PhDAllogen Lab, Cleveland Clinic

Xiaohai (Sam) Zhang, PhDCedars-Sinai Health System

Emmanuel Zorn, PhDColumbia University Medical Center

Yizhou Zou, MD, PhDCentre South University China

ABSTRACT REVIEWERS


PRESIDENTAnat R. Tambur, DMD, PhD, D(ABHI) (2016)Northwestern University303 E Chicago Ave11-711 Tarry BuildingChicago, IL 60811Phone: 312-503-2949Fax: [email protected]

PRESIDENT-ELECT

Michael D. Gautreaux, PhD, D(ABHI) (2017)Wake Forest Univ Health SciencesHLA/Immunogenetics Lab145 Kimel Park Dr., Suite 250Winston-Salem, NC 27103Phone: 336-716-4456Fax: [email protected]

PAST PRESIDENT

Malek Kamoun, MD, PhD (2015)University of Pennsylvania3400 Spruce St HUP7.020 Founders Pav Dept PathPhiladelphia, PA 19104-4283Phone: 215-614-1902Fax: [email protected]

VICE PRESIDENT OF OPERATIONS

Doreen A. Jezek, MS, CHS, MB(ASCP)CM (2017)[email protected]

SECRETARY

David Kiger, CHS(ABHI) (2016)Wake Forest University Health SciencesHLA/Immunogenetics Lab145 Kimel Park Drive, Suite 250Winston-Salem, NC 27103Phone: 336-716-1816Fax: [email protected]

TREASURER

Walter Herczyk, MT(ASCP), CHS(ABHI) (2018)Gift of Life MichiganLaboratory Administrative Director3861 Research Park DriveAnn Arbor, MI 48108-2217Phone : (734) [email protected]

2016 BOARD OF DIRECTORS

2016 EXECUTIVE COMMITTEE


Patricia M. Campbell, MBChB, FRCP (2016)University of Alberta HospitalsDept of Medicine 4B4.27 WMHSC8440 112 StEdmonton, AB T6G 2B7CANADAPhone: 780-407-7579Fax: [email protected]

John L. Schmitz, PhD, D(ABMLI, ABHI) (2016)UNC Chapel Hill101 Manning DriveRoom 1035 East WingChapel Hill, NC 27514Phone: (919) [email protected]

Cynthia Taves, PhD, D(ABHI) (2016)Lab CorpDNA Identity Testing1440 York Court ExtensionBurlington, NC 27215Phone: 336-436-7546Fax: [email protected]

Sharon Adams, MT(ASCP), CHS(ABHI) (2017)National Institute of Health10 Center Drive, MSC 1184Building 10, Room 1C744Bethesda, MD 20892Phone: 301-496-8852Fax: [email protected]

Medhat Z. Askar, MD, PhD, D(ABHI) (2017)Baylor University Medical Center3500 Gaston AveTransplant Immunology Dept4th Floor Y-WingDallas, TX 75246Phone: [email protected]

Brian Duffy, MA, CHS (ABHI) (2017)Barnes-Jewish HospitalHLA Lab Mail Stop 90-23-335One Barnes Hospital PlazaSt. Louis, MO 63110Phone: 314-362-5323Fax: [email protected]

Susan Saidman, PhD, D(ABHI) (2017)Massachusetts General Hospital Histocompatibility Lab55 Fruit St Rm GRJ 220Boston, MA 02114Phone: 617-724-3767FAX: [email protected]

Ruby Siegel, MS, CHS (ABHI) (2017)Stanford Histocompatibility, Immunogenetics & Disease Profiling Laboratory3373 Hillview AvePalo Alto, CAPhone: [email protected]

Linda S. Buckert, MT(ASCP), SBB, CHS (2018)Loma Linda University Medical Center11234 Anderson St., Rm 2125Loma Linda, CA 92354-2870Phone: (909) [email protected]

2016 BOARD OF DIRECTORS


DIAMOND LEVELOne Lambda Inc., A Thermo Fisher Scientific Brand

Pocket Schedule

PLATINUM LEVELIllumina, Inc.

Cyber Café

Immucor Lanyards

GOLD LEVELCareDx, Inc.

Water Bottles

Histogenetics Registration Bags

SILVER LEVELGenDx

Tulip Run

Linkage Biosciences Inc. Distinguished Service Award

Omixon Scholar Award

BRONZE LEVELBio-Rad Laboratories

Distinguished Scientist Award

Elsevier International Scholar Award

National Marrow Donor Program Outstanding Technologist Award

STEMCELL Technologies Inc. Rose Payne Award

USB Products by Affymetrix Scholar Award

FRIENDS LEVELMLC Group, LLC

Outstanding Technologist Dinner

CORPORATE SUPPORT


EXHIBITOR BOOTH NUMBERSAmerican Foundation for Donation & Transplantation . . . . . . . . . . . . . . . . . . . . . 509

BD Biosciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .518

Beckman Coulter Life Sciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220

Bio-Rad Laboratories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 308

CareDx, Inc. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 302

CEDARLANE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .516

chemagen from PerkinElmer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209

Discovery Healthcare Consulting Group . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .416

GenDx . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .210

GenTrak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .217

Histogenetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 409

HLA Data Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .419

Illumina, Inc. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .314

Immucor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .403 & 503

IntelliCyt Corporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .319

JETA Molecular . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .418

LabCorp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 202

Linkage Biosciences™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .313

MC Diagnostics Ltd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .318

Mid-America Transplant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .513

Miltenyi Biotec . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211

National Marrow Donor Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .515

Omixon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .415

One Lambda, A Thermo Fisher Scientific Brand . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303

Oxford Nanopore Technologies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .519

Path-Tec . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215

Promega Corporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 511

Protrans GmbH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204

Scisco Genetics, Inc. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317

STEMCELL Technologies Inc. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .514

SystemLink, Inc. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .412

UCLA Immunogenetics Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .219

USB Products by Affymetrix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 507

EXHIBITOR INFORMATION


EXHIBITOR COMPANY DESCRIPTIONSAmerican Foundation for Donation & Transplantation 509

8154 Forest Hill Ave #3 Richmond, VA 23235 USAPhone: 804-323-9893Email: [email protected]: www.amfdt.org

The American Foundation for Donation & Transplantation, formerly SEOPF, is the continuation of the oldest transplantation and donation professional organization in the United States. AFDT’s services include: educational courses for transplant professionals (Basic & Specialist Histocompatibility Courses), insurance for transplant professionals, procurement billing, travel awards and living kidney donor insurance program. www.amfdt.org. 1-800-KIDNEY9.

BD Sciences 518

2350 Qume DriveSan Jose, CA 95131 USAPhone: 408-421-9886Email: [email protected]: http://www.bdbiosciences.com

BD is a leading medical technology company that benefits countless lives worldwide. We advance health by improving the ways that discovery, diagnostics and delivery of care are conducted. With 45,000 employees at work in more than 50 countries, we work in close collaboration with customers and partners to develop innovate products and solutions that enhance outcomes, better manage healthcare delivery costs, increase efficiencies, improve healthcare safety, and expand access to health. Because we’ve been doing this for over 100 years, our portfolio, leadership and partnerships make a difference for global healthcare.


EXHIBITOR COMPANY DESCRIPTIONS (CONTINUED)

Beckman Coulter Life Sciences 220

5350 Lakeview Parkway S DriveIndianapolis, IN 46268 USAPhone: 800-742-2345Email: [email protected]: http://www.beckman.com

Beckman Coulter Life Sciences is dedicated to improving the health of people around the world. The company’s global leadership and world-class service and support delivers sophisticated instrument systems, reagents and services to life science researchers in academic and commercial laboratories, enabling new discoveries in biology-based research and development. A leader in centrifugation and flow cytometry, Beckman Coulter has long been an innovator in capillary electrophoresis, particle characterization and laboratory automation, and its products are used at the forefront of important areas of investigation, including genomics and proteomics. For more information, please visit www.Beckman.com.

Bio-Rad Laboratories 308

4000 Alfred Nobel Drive Hercules, CA 94547 USA Phone: 1-800-BIORAD Email: [email protected] Website: www.bio-rad.com

Bio-Rad offers a complete line of industry standard HLA Serology Typing Trays (Lymphotype); Serological (Lymphoscreen) and ELISA-based (AbScreen/Abldent*) Antibody Diagnostics Systems; Molecular Typing Systems (SSO and SSP) with full automation platforms; Immune Monitoring, Infectious Disease and Traditional Blood Group Serology Products to meet your laboratory needs.

*Not available in U.S.



CareDx, Inc. 302

3260 Bayshore Blvd.Brisbane, CA 94005 Phone: 1-888-255-6627Email: [email protected]: www.caredx.com CareDx, Inc., headquartered in Brisbane, California, is a global molecular diagnostics company focused on the discovery, development and commercialization of clinically differentiated, high-value diagnostic solutions for transplant patients. CareDx offers testing products across transplantation, including AlloMap® and AlloSure™ for post-transplant surveillance and Olerup SSP®, XM-ONE®, QTYPE®, Gamma-Type™ and SBT Resolver™ in pre-transplant testing. Additional information is available at www.caredx.com.

CEDARLANE 516

1210 Turrentine Street Burlington, NC 27215 USA Phone: 800-721-1644Email: [email protected] Website: www.cedarlanelabs.com CEDARLANE is a leading distributor and manufacturer of quality reagents to the transplant and research communities. We specialize in producing Complement for tissue typing and bactericidal assays. Other manufactured products include Lympholyte cell separation media and Antibodies including our B and T cell Positive Control Antisera and antibodies to complement components and TdT.



chemagen from PerkinElmer 209

710 Bridgeport Ave Shelton, CT 06484 USA Phone: 202-402-1994 Website: www.chemagen.com

chemagen a leading supplier of automation and reagents for fast and reliable magnetic bead based DNA and RNA extraction for sample volumes from 10 ul to 10 ml for blood, tissues, saliva, bacteria, food, PCR products. All functions can be performed on the one instrument. Advantages of this unique system are fast processing, unmatched sample volume range and robust chemistry.

Discovery Healthcare Consulting Group 416

909 E. 18th Street Plano, TX 75074 USA Phone: 972-312-9102 Email: [email protected] Website: DHCG.com Transplant Solutions LLC and Discovery Healthcare Consulting Group have formed a unique affiliation to provide consulting services to the transplant community. We offer a full spectrum of Organ Procurement Organization/Histocompatibility Laboratory Cost Reporting consulting services designed to ensure full reimbursement and compliance. Our services include cost report compilation and analysis, compliance reviews, CMS audit support, cost report data accumulation procedures review and special projects related to OPO/Histocompatibility Lab cost reports. For hospital based transplant centers we provide consulting services on all matters related to government reimbursement and CMS requirements including Conditions of Participation.



GenDx 210

1448 South Rolling Road Baltimore, MD 21227 USA Phone: 443-543-5254 Email: [email protected]: www.gendx.com

The HLA Sequencing Experts

Expertise in workflows for HLA typing and sequencing data analysis characterise GenDx as innovator and market leader in the HLA typing field. Developing new solutions and offering excellent support and education to HLA labs are our primary objectives. Find out more about products and services:

• NGSgo® & NGSengine®

• SBTexcellerator®, GenDx-AlleleSEQR® & SBTengine®

• qPCR Chimerism monitoring with KMRengine®

For more info contact our local US office in Baltimore, MD:www.gendx.comphone: 443 543 [email protected]

GenTrak 217

PO Box 1290 Liberty, NC 27298 USA Phone: 336-622-5266 Emai: [email protected] Website: www.gentrakinc.com

GenTrak, Inc. manufactures a quality line of classical HLA serology trays and Frozen Cell trays. HLA serology testing provides quick, cost effective results as well as useful information for molecular typing. Please come see our SSP products, including BAG Cycler check, ABO Typing and Wipetest. We also represent TBG Biotechnology products for ABCDRDQDP DNA Sequencing.



Histogenetics 409

300 Executive Blvd Ossining, NY 10562 USA Phone: 914-762-0300 Email: [email protected]: www.histogenetics.com

Histogenetics, based in New York, USA, is an international leader in DNA sequence-based tissue typing (SBT) services. Histogenetics mastered Next Generation Sequencing (NGS) and Third generation sequencing technologies (PacBio) for routine HLA typing for ultimate resolution and quality at high volume. Histogenetics has been working with the world’s largest donor centers, National Marrow Donor Program (NMDP), German Donor Center (DKMS), Office of Naval research (ONR)and many other registries.

HLA Data Systems 419

12335 Kingsride Lane #411 Houston, TX 77024 USA Phone: 832-699-5253 Email: [email protected] Website: www.mtilda.com HLA Data Systems delivers data solutions for the histocompatibility and immunogenetic community. Our product suite includes “mTilda,” a robust and comprehensive Laboratory Information System, “NextGen Connect” an integrated NGS data pipeline solution, “VXmatch,” a visually-rich virtual crossmatch web application appropriate for lab and clinic personnel, as well as “3Ring” a centralized, digital tracker for competency of personnel, proficiency testing, and validation. We provide interfaces with all our products, and subscribe to the highest standards of interoperability.



Illumina, Inc. 314

5200 Illumina Way San Diego, CA 92122 USA Phone: 858-202-4500 Email: [email protected] Website: www.illumina.com

Illumina is improving human health by unlocking the power of the genome. Our focus on innovation has established us as the global leader in DNA sequencing and array-based technologies, serving customers in the research, clinical and applied markets. Our products are used for applications in the life sciences, oncology, reproductive health, agriculture and other emerging segments. To learn more, visit www.illumina.com and follow @illumina.

Immucor 403 & 503

3130 Gateway Drive Norcross, GA 30071 USA Phone: 855-466-8267 Email: [email protected]: www.immucor.com

For more than 30 years, Immucor has focused on improving patient outcomes by bringing together best-in-class solutions developed to improve donor selection and lower the probability of rejection. In addition to offering market-leading assays for typing and screening blood used in life-saving transfusions, we offer the most comprehensive — and ever-expanding — portfolio of products and technologies to support transplant teams across the continuum of care. Our transplant diagnostics division provides molecular and antibody-based assays for HLA compatibility between donors and recipients as well novel assays for post-transplant surveillance.



IntelliCyt Corporation 319

9620 San Mateo Blvd NEAlbuquerque, NM 87113 USAPhone: 505-345-9075Email: [email protected]: www.intellicyt.com

IntelliCyt’s iQue Screener platform is an integrated instrument, software, and reagent solution that offers rapid, multiplexed analysis of cell and bead-based samples in 96 well plates. The iQue Screener’s proprietary sampling technology uniquely enables rapid assessment of cells in suspension by miniaturizing the assay format, which conserves precious samples for further analysis. The powerful ForeCyt Software-assisted automation, analysis, and experiment-level visualization tools reveal deep insight into complex biology. Our easy-to-use integrated solution delivers more insight from less sample, in the least amount of time, providing a cost-effective means for making better decisions.

JETA Molecular 418

Krommewetering 101CUtrecht 3543AN The NetherlandsPhone: +31654136697Email: [email protected]: jetabv.com

JETA creates innovative reagent and software tools for personalized stem cell and solid organ transplant monitoring.



LabCorp 202

1440 York Court Burlington, NC 27215 USA Phone: 336-436-7418 Email: [email protected] Website: www.labcorp.com

Laboratory Corporation of America® Holdings, an S&P 500 company, is the world’s leading healthcare diagnostics company, providing comprehensive clinical laboratory services through LabCorp Diagnostics, and end-to-end drug development support through Covance Drug Development. LabCorp is a pioneer in commercializing new diagnostic technologies and is improving people’s health by delivering the combination of world-class diagnostics, drug development and knowledge services. LabCorp provides leading-edge medical laboratory services through a sophisticated network of primary clinical and specialty testing laboratories. LabCorp consist of 48,000 employees worldwide, providing scientific expertise in esoteric testing, genomics and clinical and anatomic pathology. LabCorp’s Identity Testing Division offers comprehensive HLA Next Generation Sequencing (NGS) technology, HLA Antibody Analysis, Pre/Post Engraftment Monitoring, and Bio-sample Processing services in support of international donor registries, transplant centers, research and pharmaceutical companies.

Linkage Biosciences™ 313

890 Dubuque Avenue South San Francisco, CA 94080 USA Phone: 415-346-5262 Email: [email protected] Website: www.linkagebio.com

Linkage Biosciences™ is a molecular diagnostics company developing and marketing products that dramatically improve and expedite complex genetic testing. LinkSēq™ is the company’s Human Leukocyte Antigen (HLA) testing product line. HLA testing is used in solid organ and hematopoietic stem cell transplantation as well as pharmacogenomic and autoimmune disease testing and research. Linkage Biosciences is headquartered in South San Francisco, California.



MC Diagnostics Ltd 318

Unit 5OpTICFordd William MorganSt. Aspah, Denbighshire UK LL17 0JDPhone: +44 1745 535225Email: [email protected]: www.mcdiagnostics.co.uk

The company has developed a fully automated platform for molecular diagnostics. A full range of HLA typing kits are available and a new product will be introduced, which uses a single test, specifically designed to identify 23 different HLA alleles linked to Adverse Drug Reactions.

Mid-America Transplant 513

1110 Highlands Plaza DrE #100St. Louis, MO 63110 USAPhone: 314-735-8200Email: [email protected]: https://www.midamericatransplant.org/

Mid-America Transplant changes lives for the better. Since 1974, Mid-America Transplant has facilitated the organ and tissue donation process for a service area of 4.7 million people in eastern Missouri, southern Illinois, and northeast Arkansas. With a mission to save lives through excellence in organ and tissue donation, Mid-America Transplant compassionately provides care for donors, transplant recipients and their families. To drive the organization’s mission, we build strong partnerships with 122 hospitals, four transplant centers, seven tissue specialists and multiple community organizations. Committed to an innovative approach, Mid-America Transplant is the first OPO in the nation to utilize an in-house operating room model for organ recovery and the first to operate license offices to increase community support through donor registry enrollment. Throughout the organization’s history, the workforce has relentlessly pursued its core values of compassion, quality, teamwork, integrity, and innovation to pursue a vision that organs and tissues are always available to those in need. Located in St. Louis, MO, Mid-America Transplant is a not-for-profit organization and is one of 58 federally designated organizations of its kind in the United States.



Miltenyi Biotec 211

Friedrich-Ebert-Strasse 68 Bergisch Gladbach NRW 51429 Germany Phone: 4.92205E+13 Email: [email protected] Website: www.miltenyibiotec.com

Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. Used by scientists and clinicians around the world, our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, and cell culture. Our 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer. Today, Miltenyi Biotec has more than 1,500 employees in 25 countries – all dedicated to helping researchers and clinicians make a greater impact on science and health.

National Marrow Donor Program 515

500 N 5th St. Minneapolis, MN 55401 USA Email: [email protected] Website: https://bethematch.org The National Marrow Donor Program® (NMDP)/Be The Match® is a global leader in providing a cure to patients with life-threatening blood and marrow cancers such as leukemia and lymphoma, as well as other diseases. We match patients with donors, educate health care professionals and conduct research through the CIBMTR, our research program, so more lives can be saved. We offer services in HLA and KIR data analysis and integration. Visit our booth to learn more about how we can partner to achieve your goals.



Omixon 415

Budapest, 1117 Hungary Phone: 3620-3557223 Email: [email protected] Website: omxion.com

Omixon is a global molecular diagnostics company, empowering clinical labs with technologies to improve patient outcomes by commercializing disruptive innovations specializing in targeted applications for Next Generation Sequencing (NGS). The Omixon Holotype HLA™ product combines a targeted HLA Assay and the Omixon HLA Twin™ software to deliver the most accurate high-resolution HLA genotyping available. Omixon maintains an active grant-funded research program and assists scientists and clinicians to analyze the most challenging genomic regions including HLA, KIR and ABO.

One Lambda, A Thermo Fisher Scientific Brand 303

21001 Kittridge Street Canoga Park, CA 91303 USA Phone: 818-702-0042 Email: [email protected] Website: www.onelambda.com

One Lambda, Inc., a Thermo Fisher Scientific brand, is a global leader in transplant diagnostics offering a full range of HLA Typing and antibody monitoring products to support clinicians and laboratories in the management of transplant patients. Visit www.onelambda.com to discover how we can help you improve patient outcomes.

Contact: Ricardo OrdonezAddress: 21001 Kittridge StreetTelephone: 1.818.702.0042Email: [email protected]: www.onelambda.comCanoga Park, CA 91303



Oxford Nanopore Technologies 519

Edmund Cartwright House4 Robert Robinson Avenue, Oxford Science ParkOxford, OX4 4GA EnglandPhone: 0845 034 7900 ext: 1926Email: [email protected]: www.nanoporetech.com

Oxford Nanopore Technologies™ is developing a new generation of nanopore-based electronic systems for analysis of single molecules, including DNA, RNA and proteins. The MinION™ device, the PromethION™ and GridION™ systems are designed to provide novel qualities in molecular sensing such as real-time data streaming, improved simplicity, efficiency and scalability of workflows and direct analysis of the molecule of interest. The devices may be used in scientific research, personalised medicine, crop science, security and defence and environmental applications. Products and services are priced for low total-cost-of-ownership DNA sequencing (or other analyses). www.nanoporetech.com

Path-Tec 215

5700 Old Brim Road Midland, GA 31820 USA Phone: 706-507-1514 Email: [email protected] Website: www.Path-Tec.com

Path-Tec has been delivering innovative specimen and client management solutions since 2005, and we understand the challenges that laboratories encounter with their pre-analytical processes. Our client support solutions include supply and kit management, inventory management, specimen and supply logistics, and client management technology.When partnering with Path-Tec, laboratories benefit from our specimen management expertise to reduce costs, improve client service levels, and improve overall operational efficiencies. We are committed to helping your laboratory thrive in today’s changing healthcare landscape without sacrificing quality or service.



Promega Corporation 511

2800 Woods Hollow Road Madison, WI 53711 USAPhone: 608-274-4330 Email: [email protected] Website: www.promega.com Contributing to Science, Discovery and More. Founded in 1978, what started as the production of enzymes for researchers has evolved to over 3,000 products for a broad array of applications including basic research, drug discovery, forensics and paternity testing and molecular diagnostics. Our portfolio includes IVD and RUO products, as well as instruments and reagents for DNA and RNA extraction, PCR, STR analysis, genetic analysis and mutation detection.

Protrans GmbH 204

Ketschau 2 Hockenheim B-W 68766 Germany Phone: 49-6205292990 Email: [email protected]

Protrans medizinische diagnostische Produkte GmbH, Hockenheim Germany: Unique Allele-Specific Sequencing Strategies. Highest Resolution Results in one day. You are welcome at our booth 204.

Scisco Genetics, Inc. 317

PO Box 9245 Seattle, WA 98109 USA Phone: 206-890-2304 Email: [email protected] Website: sciscogenetics.com

Scisco Genetics is dedicated to providing immunogenetics laboratories with next-generation sequencing (NGS) solutions that will simultaneously expand testing capabilities and simplify laboratory, data analysis, and reporting procedures. Our Integrated Genotyping System (IGS) is designed to enable clinical laboratories to make a smooth transition to NGS for all of their testing needs.



STEMCELL Technologies Inc. 514

1618 Station St Vancouver, BC V6A1B6 Canada Phone: 604-675-7785 Email: [email protected]: www.stemcell.com

STEMCELL Technologies provides easy-to-use cell isolation solutions for flow crossmatch and chimerism analysis, facilitating rapid sample processing and reliable results. EasySep™ and RosetteSep™ are fast, gentle on cells and stable at room temperature. To save technician time, RoboSep™ fully automates EasySep™ isolation and, SepMate™, registered as an IVD device, isolates PBMC in just 15 minutes.

SystemLink, Inc. 412

1939 Roland Clarke Place Suite 340 Reson, VA 20191 USA Phone: 703-651-5700 Email: [email protected]: www.HistoTrac.com Visit the SystemLink booth to learn about building a complete, customizable HLA laboratory management system to meet the needs of your laboratory. HistoTrac is a seamless system with flexible design, providing functionality to make data entry and data access quick and easy. Come learn more about:• HistoTrac on the Web - Web-based access to patient data• Paired Kidney Exchange module• TIEDI module• NGS interfaces



UCLA Immunogenetics Center 219

1000 Veteran Avenue Room 1-520 Los Angeles, CA 90095 USA Phone: 310-825-7651 Email: [email protected]: HLA.UCLA.EDU

The UCLA Immunogenetics Center (UIC) provides comprehensive testing for organ and tissue transplantation. Transplant testing has a long history at UCLA. HLA typing was pioneered here in the 1960’s. The development of the microcytoxicity test in 1964 marked the beginning of international testing and standardization of HLA typing. The UCLA Immunogenetics Center has retained its leadership position in HLA research, and in the development of accompanying diagnostic testing. In the intervening years, laboratory and scientific personnel, from the US and abroad, have learned HLA typing through courses and workshops at the UCLA Immunogenetics Center Laboratory. The center provides Immunogenetics and Histocompatibility testing services, as well as other testing services, to a wide range of physicians, patients, pharmaceutical companies and other medical facilities. The laboratory is licensed by the States of California, New York, Florida, Maryland, Pennsylvania and Rhode Island, is CMS certified, and accredited by the American Society for Histocompatibility and Immunogenetics.

USB Products by Affymetrix 507

26111 Miles Road Cleveland, OH 44128 USA Phone: 800-321-9322 Email: [email protected] Website: www.usb.affymetrix.com

Affymetrix|USB has helped hospital labs, universities, biotech, and pharmaceutical companies excel for more than 40 years. Our molecular biology reagents, enzymes and biochemicals deliver reliable and repeatable results. Stop by to learn how to fast track your sequencing results with ExoSAP-IT Express reagent, our new 5 minute enzymatic PCR cleanup method.


Monday, September 26, 2016

8:30 am – 4:30 pm GenDx NGS Teaching Session

Sterling 3, Level Two 9:30 am – 11:00 am Omixon: Introduction to

NGS-based HLA-typing with HLA Twin

Mills 4, Level Four

11:30 am – 1:00 pm Omixon: Resolving Complex Cases of NGS-based HLA-typing with HLA Twin

Mills 4, Level Four

2:00 pm – 4:30 pm GenDx Chimerism Teaching Session

Sterling 6, Level Two

AFFILIATE AND USER GROUP MEETINGSThere are a number of affiliate and user group meetings taking place during the ASHI 42nd Annual meeting. These are open to all attendees. Although not directly affiliate with ASHI, we urge you to attend and support these additional educational opportunities.

Tuesday, September 27, 2016

11:30 am – 1:30 pm Steering the Patient Journey Toward Improved Outcomes Via an Evolving Spectrum of Pre- and Post-Transplant Diagnostic Solutions

Supported by Immucor Regency Ballroom F

Real World Experiences with Real-Time PCR HLA Typing and Transplantation of Highly Sensitized Patients and the Importance of SABRTM (Single Antigen Bead Resolution) Typing Kits

Linkage Biosciences Regency Ballroom E

Comprehensive Monitoring of Anti-HLA Antibody and its Clinical Impact on Rejection and Graft Outcome

Supported by One Lambda A Thermo Fisher Scientific Brand

Park View

Wednesday, September 28, 2016

12:30 pm – 2:30 pm CareDx Lunch Symposium: Advancing the Pre- and Post-Transplant Continuum

Supported by CareDx, Inc. Regency Ballroom E

Looking to the Future of Immunogenetics with NGS

Supported by Illumina Regency Ballroom F

One Lambda NXType Assay for your Clinical NGS Application

Supported by One Lambda A Thermo Fisher Scientific Brand

Park View

Thursday, September 29, 2016

12:30 pm – 2:30 pm HLA Typing and Beyond – NGS & Chimerism

Supported by GenDx Regency Ballroom E

NGS Accreditation with ASHI Standards

Supported by Omixon Regency Ballroom F

New and Innovative Tools to Improve Your Flow Crossmatch Assay (FCXM) Workflow

Supported by STEMCELL Technologies

Park View

AFFILIATE MEETINGS

USER GROUP MEETINGS


The Paul I. Terasaki Clinical Science Award was established in 2003 to honor an individual, group, or institution in recognition of significant accomplishments and/or contributions to the fields of clinical transplantation, histocompatibility and immunogenetics. This award was made possible by a grant from the Paul I. Terasaki Foundation.

The winner of the 2016 Paul I. Terasaki Clinical Science Award is:

Peter Nickerson, MD, FRCPCUniversity of Manitoba

Dr. Peter Nickerson is a Distinguished Professor of Internal Medicine and Immunology and the Vice Dean (Research), Faculty of Health Sciences at the University of Manitoba. He is the Medical Director of Transplant Manitoba, Medical Advisor, Organ Donation and Transplantation Division,

Canadian Blood Services (CBS), and Medical Consultant for Diagnostic Services of Manitoba – Transplant Immunology Laboratory.

Dr. Nickerson holds the Flynn Family Chair in Renal Transplantation at the University of Manitoba. Funded by the CIHR and NIH, his research program focuses on mechanisms underlying acute and chronic transplant rejection; developing non-invasive techniques for the diagnosis of renal allograft rejection; and health care system design to enhance access to transplant.

THE PAUL I. TERASAKI CLINICAL SCIENCE AWARD


The Rose Payne Award was established in 1984 to honor a great scientist and to recognize her longstanding contributions to the field of immunogenetics. As a founding member of ASHI, Dr. Rose Payne was always willing to share her knowledge and assist others in their endeavors. For more than 30 years, Dr. Payne made significant contributions in areas related to HLA. The Rose Payne Award was established as a tangible recognition of the high regard in which she was held.

The winner of the 2016 Rose Payne Award is:

Rene J. Duquesnoy, PhDUniversity of Pittsburgh Medical Center

Rene J. Duquesnoy was born in the Netherlands where he obtained a graduate degree in chemical engineering from the Technological University of Delft. He received a Ph.D. degree in Pathology from the University of Tennessee in Memphis and did a post-doctoral fellowship with Robert

A. Good at the University of Minnesota. After his affiliation with the Medical College of Wisconsin and the Blood Center of Wisconsin, he became a tenured Professor of Pathology, with joint appointments in Immunology and Surgery at the University of Pittsburgh Medical Center. Since 2009 he is Professor Emeritus of Pathology.

Dr. Duquesnoy’s research deals with HLA and transplantation immunology. His curriculum vitae have more than 400 publications and 500 scientific presentations at national and international meetings. Milestones include cross-reactive HLA matching in platelet transfusions, the discovery of the MB system (now called HLA-DQ), alloreactivity of graft-infiltrating T-cells, the dualistic role of HLA in liver transplantation, role of stress proteins in transplant immunity, antibody analysis of highly sensitized patients and more recently, HLA epitopes and the computer algorithm called HLAMatchmaker.

Dr. Duquesnoy was ASHI president in 1986-87, served on numerous ASHI committees and directed the ASHI PT program in 1984-2007. He serves on the editorial boards of Human Immunology, Transplantation and several other professional journals.

THE ROSE PAYNE AWARD


The ASHI Distinguished Scientist Award was established in 2001 to honor a distinguished scientist who is an ASHI member. This individual must have contributed significantly to the field of immunogenetics and/or transplant immunobiology.

The winner of the 2016 ASHI Distinguished Scientist Award is:

Malek Kamoun, MD, PhDUniversity of Pennsylvania

Dr. Kamoun received his training in human immunogenetics in Dr. Jean Dausset’s laboratory in Paris, France. He received his Medical Degree at the Université de Pierre et Marie Curie in Paris, France. He is a Professor of Pathology and Laboratory Medicine at the University Of

Pennsylvania School Of Medicine. He is Board-certified in Clinical Pathology and Immunopathology. He is the Director of the Immunogenetics and Clinical Immunology and Histocompatibility Laboratories at PENN. He has dedicated his research career to investigations focused on human immunogenetics, lymphocyte biology and clinical transplantation research. He has made numerous significant contributions to these areas during the last 37 years and has co-authored over 150 peer reviewed scientific papers. In addition, he served as PI or Co-PI on several previous and current NIH-funded grants focused on the immunobiology of transplantation and autoimmune disease. His current research interest is to understand the biological relevance of amino acid polymorphism in HLA class I and class II molecules. Computational and biostatical methods are used to evaluate the significance of HLA amino acid polymorphism in clinical transplantation and autoimmunity. Another interest is to better understand the impact of B cell alloresponse and anti-HLA antibodies on allograft outcomes in clinical transplantation including islet cell transplantation. Clinical trials are used to assess the effectiveness of B cell depleting agents on alloantibody levels and allograft survival in transplant recipients.

ASHI DISTINGUISHED SCIENTIST AWARD


The ASHI Distinguished Service Award was established in 1999 to honor colleagues who have contributed significantly to serving ASHI.

The winner of the 2016 Distinguished Service Award is:

Amy B. Hahn, PhD, D(ABHI)Albany Medical College Transplant Immunology Laboratory

Amy Hahn has been the director of the Albany Medical College Transplant Immunology Laboratory since 1997 and is a Professor in the Department of Surgery. She is also the offsite director of the Tissue Typing Laboratory at

Upstate Medical University Hospital in Syracuse. Her career in HLA began when she was a college student at Carnegie-Mellon University and worked as a summer research student in the University of Pittsburgh Immunopathology Laboratory which was then directed by Bruce Rabin. After doing her PhD thesis at Johns Hopkins on the mouse non-classical MHC molecule Qa-2, she was lucky enough to secure a spot as a post-doc and director trainee at the Johns Hopkins Immunogenetics Laboratory. While there she had the opportunity to be trained by and work with some of the pillars of the histocompatibility and immunogenetics community, including Wilma Bias, Susie Leffell, Andrea Zachary, John Hart, Donna Lucas and Susan Hsu. It was through their influence that Amy joined ASHI. After leaving Hopkins to become a lab director in Charleston SC, Amy became a member of the ASHI Publications Committee in 1995. This began a commitment to serving ASHI that has lasted more than 20 years and has included terms as chair of the publications, external affairs, national affairs, and program planning committees; service as an ARB inspector and commissioner; terms on council and the board of directors; and serving as president. She has also served ASHI as editor-in-chief of the ASHI Quarterly, co-editor of the 4th edition of the laboratory manual, co-editor of the 25th anniversary commemorative book, and recently assumed the position of editor-in-chief of Human Immunology after many years as a reviewer, editorial board member and section editor. In addition, she has served on multiple committees and the boards of ABHI and UNOS. Amy is thrilled and honored to have been chosen for this award and thanks those who nominated her and wrote letters of support. She also thanks the many other ASHI volunteers she’s worked with and the ASHI office staff for all their help. Most importantly, she’d like to thank her lab staff and family who have supported her work for ASHI and tolerated her occasional absences and divided attention.

ASHI DISTINGUISHED SERVICE AWARD


The Outstanding Technologist Award has honored some of the most active and creative technologists in the field of HLA. Candidates must have made significant and sustained contributions to ASHI.

The winner of the 2016 Distinguished Service Award is:

Benita K. Book, MS, CHS (ABHI), MT (ASCP), CCRC (ACRP)

Benita (Bennie) K. Book began her career in histocompatibility in the laboratory of Dr. Angenita Biegel at Indiana University. There I learned the basics from my supervisor Bob Bray. You would know him as Dr. Robert Bray, ASHI past president. During this time I was a member

of AACHT that then became AHSI. The opportunity to move to the Transplant Immunology Laboratory in the Department of Surgery at IU became available. There my real education began. Drs. Stephen Leapman and Ronald Filo taught me about transplantation from their clinical point of view. They were wonderful mentors who encouraged me to go to graduate school and expand my thinking. I thought I had meet the two smartest men in the world and then Dr. Mark Pescovitz joined us with his brilliant mind. While I learned more about research, new techniques and transplantation, I continued involvement in ASHI. First I was co-editor of the ASHI Quarterly with my dear friend Joan Bray. There are those who still run when they see me afraid I will request an article for the Quarterly from them. I was also on the ABHI exam committee and then went on to become president of ABHI. I can proudly say during that time we moved the ABHI exams from pencil and paper to electronic format. Rarely have I missed an ASHI meeting and continue to learn from its wonderful members many of whom are also my dear friends.

ASHI OUTSTANDING TECHNOLOGIST AWARD


The ASHI Rising Star Award (formerly the ASHI/AFDT J. Marilyn MacQueen Award) recognizes an outstanding HLA technologist who has worked in the field for fewer than three years and desires topursue a career in HLA.

The winner of the 2016 Rising Star Award is:

Tia LaBarge, BS, MTUniversity of Rochester Medical Center

Tia LaBarge is a Medical Technologist Specialist in the HLA laboratory at the University of Rochester Medical Center in Rochester, NY. She has been employed by the URMC Department of Pathology and Clinical Medicine for 12 years. Tia earned her Bachelor’s degree in Biology

from Ithaca College in Ithaca, NY in 2004 and is a licensed New York State Medical Technologist. She is an active member of the URMC “ICare” and Patient Experience committees and has been frequently recognized by her colleagues for her commitment to this cause. Tia enjoys reading, traveling and spending time with her family. She currently resides in Rochester, NY with her husband and three children.

ASHI RISING STAR AWARD


The best abstracts submitted for the 2016 Annual Meeting will be recognized during the Awards Symposium and the submitters will give an oral presentation during the Special Abstract Scholar Session. These abstracts received the highest rating by the reviewers, and the awards are providedto recognize individuals who made a significant advance in either clinical or basic research areas. The authors were selected from more than 200 submitted abstracts. Recipients receive a monetary award and certificate of recognition for their research.

SPECIAL ABSTRACT SESSION: SCHOLAR AWARDS

Hidetoshi Inoko, PhD – ASHI International ScholarGenoDive Pharma Inc., Kanagawa, Japan

OR15NEW NGS HLA TYPING BY TARGETED ENRICHMENT PROCEDURE, CAPTURE METHODHidetoshi Inoko1, Yuko Okudaira1, Anri Masuya1, Atsushi Tajima2, Kazuyoshi Hosomichi2, 1GenoDive Pharma Inc., Honatusgi, Kanagawa, JAPAN, 2Kanazawa University, Kanazawa, Ishikawa, JAPAN.

Muthukumar Gunasekaran, PhD – ASHI ScholarSt. Joseph’s Hospital & Medical Center, Norton Thoracic Institute, Phoenix, AZ, USA

OR16ANTIBODIES TO DONOR HUMAN LEUKOCYTE ANTIGEN INDUCE EXOSOMES CONTAINING TISSUE-RESTRICTED SELF-ANTIGENS IN ACUTE AND CHRONIC REJECTIONMuthukumar Gunasekaran1, Monal Sharma1, Ramsey Hachem2, Rajat Walia1, Surendra Shenoy3, Michael Smith1, Ross Bremner1, Thalachallour Mohanakumar1, 1St. Joseph’s Hospital & Medical Center, Norton Thoracic Institute, Phoenix, AZ, 2Washington University School of Medicine, Medicine, St. Louis, MO, 3Washington University School of Medicine, Surgery, St. Louis, MO.

ASHI SCHOLARS & INTERNATIONAL SCHOLAR AWARDS


Nilesh S. Chitnis, PhD – ASHI ScholarChildren’s Hospital of Philadelphia, Philadelphia, PA, USA

OR17HLA-B INTRON DERIVED MICRORNA REGULATES THE POSTTRANSCRIPTIONAL EXPRESSION OF THE HEAVY CHAIN OF THE IMMUNOGLOBULIN ANilesh S. Chitnis1, Peter M. Clark1, Bradley Johnson2, Catherine Stolle1, Malek Kamoun2, Dimitri Monos1,2, 1Children’s Hospital of Philadelphia, Philadelphia, PA, 2University of Pennsylvania, Philadelphia, PA.

Cynthia Vierra-Green, MS – ASHI ScholarGeorgetown University Hospital, Immunobiology Research, Washington, DC, USA

OR18FULL GENE SEQUENCING REVEALS VERY LIMITED VARIATION IN THE REGIONS OUTSIDE OF THE ANTIGEN RECOGNITION DOMAINS (ARD) OF 360 UNRELATED HEMATOPOIETIC STEM CELL TRANSPLANT DONOR-RECIPIENT PAIRS MATCHED FOR 10/10 AT HIGH RESOLUTIONCarolyn Hurley1, Lihua Hou2, Cynthia Vierra-Green3, Colleen Brady3, Mike Haagenson3, Stephen Spellman3, 1Georgetown University Hospital, Immunobiology Research, Washington, DC, 2Georgetown University School of Medicine, Immunobiology Research, Rockville, MD, 3CIBMTR, Immunobiology Research, Minneapolis, MN.


PROGRAMMONDAY, SEPTEMBER 26, 2016

7:00 am – 8:00 pm Registration Open Grand Ballroom Prefunction, Level Four

8:00 am – 6:00 pm Immunogenetics Basic Science Symposium – separate registration fee

Regency Ballroom F, Level Two

Moderators: Megan Cooper, MD, PhD, Washington University in St. Louis, St. Louis, MO, USA and Peter Parham, PhD, Stanford University, Stanford, CA, USA

8:00 am – 8:30 am Innate Lymphoid Cells in Immunity Marco Colonna, MD Washington University School of Medicine St. Louis, MO, USA

8:30 am – 9:00 am The Ly49 NK Cell Receptors Wayne Yokoyama, MD Washington University in St. Louis St. Louis, MO, USA

9:00 am – 9:30 am Regulation of Natural Killer Cell Effector Functions by Metabolism

Megan Cooper, MD, PhD Washington University in St. Louis St. Louis, MO, USA

9:30 am – 10:00 am Novel Strategies to Activate NK Cells May Overcome Inhibitory Checkpoints to Enhance their Anti-Tumor Efficacy

Jeffrey Miller, MD University of Minnesota Minneapolis, MN, USA

10:00 am – 10:20 am Coffee Break


PROGRAMModerators: Mary Carrington, PhD, Leidos Biomedical Research Inc., Frederick, MD, USA and Raja Rajalingam, Ph.D, D(ABHI), University of California, San Francisco, San Francisco, CA, USA

10:20 am – 10:50 am The Math of KIR: Multiplication of Loci and Division of Function

Lisbeth Gutehlein, PhD Stanford University Stanford, CA, USA

10:50 am – 11:20 am Immunogenetics of Human NK Cells Peter Parham, PhD Stanford University Stanford, CA, USA

11:20 am – 11:50 am Functional Diversification of the Human NK Cell Repertoire

Karl-Johan Malmberg, MD, PhD Oslo University Hospital Oslo, Norway

11:50 am – 1:00 pm Lunch Break

Moderators: Peter Cresswell, PhD, FRS, Yale University Medical School, New Haven, CT, USA and William Hildebrand PhD, D(ABHI), University of Oklahoma Health Science Center, Oklahoma, OK

1:00 pm – 1:30 pm Unraveling the Mechanisms of Cross-Presentation Peter Cresswell, PhD, FRS Yale University Medical School New Haven, CT, USA

1:30 pm – 2:00 pm Importance of NADPH Oxidase in B Lymphocyte Function

Janice Blum, PhD Indiana University School of Medicine Indianapolis, IN, USA

2:00 pm – 2:30 pm Generalists and Specialists: A New View of How Class I Molecules Fight Disease

Jim Kaufman, PhD University of Cambridge Cambridge, United Kingdom


PROGRAM2:30 pm – 3:00 pm Immunogenetic Variation that Impacts Human Health

and Disease: A Tricky Balancing Act Mary Carrington, PhD Leidos Biomedical Research Inc., Frederick National

Laboratory for Cancer Research Frederick, MD, USA

3:00 pm – 3:20 pm Coffee Break

Moderators: Peter Parham, PhD, Stanford University, Stanford, CA, USA and Elaine Reed, PhD, D(ABHI), University of California Los Angeles, Los Angeles, CA, USA

3:20 pm – 3:50 pm Interactions Between Maternal NK cells and Fetal HLA-C Influence the Success of Pregnancy

Andrew Sharkey, PhD University of Cambridge Cambridge, United Kingdom

3:50 pm – 4:20 pm New Means for the Characterization of the MHC Dimitri Monos, PhD Univ of Pennsylvania/Children’s Hospital of

Philadelphia Philadelphia, PA, USA

4:20 pm – 4:50 pm Surprise at the Synapse: An Unexpected Role for MHCI in Neurons

Carla Schatz, PhD Stanford University Stanford, CA, USA

4:50 pm – 5:20 pm Interplay between HLA, Integrins and TLRs Mediate Endothelial Cell Activation and Chronic Inflammation in the Setting of Antibody-Mediated Rejection

Elaine Reed, PhD, D(ABHI) University of California Los Angeles Los Angeles, CA, USA

5:20 pm – 6:00 pm Open Discussion

12:00 pm – 4:30 pm Inspectors Training Workshop Regency Ballroom E, Level Two


PROGRAM5:30 pm – 6:30 pm First Time Attendee & New Members Reception Gateway Terrace, Level Eighteen

7:00 pm – 8:00 pm Scientific Award Presentation: Rose Payne Awardee Regency Ballroom ABCD, Level Two

Moderator: Malek Kamoun, MD, PhD, University of Pennsylvania, Philadelphia, PA, USA Reflections on HLA Epitope-Based Matching for

Transplantation Rene J. Duquesnoy, PhD 8:00 pm – 9:00 pm Welcome Reception with Exhibits Grand Ballroom, Level Four

TUESDAY, SEPTEMBER 27, 2016


8:00 am – 9:00 am Welcome & Keynote Address Regency Ballroom ABCD, Level Two

8:00 am – 8:05 am Welcome Malek Kamoun, MD, PhD University of Pennsylvania Philadelphia, PA

8:05 am – 9:00 am Keynote Address: Immunogenetics and Functions of MHC Molecules

John Trowsdale, PhD University of Cambridge Cambridge, Cambridgeshire, United Kingdom

9:00 am – 9:30 am Coffee Break in Exhibit Hall Grand Ballroom, Level Four

9:30 am – 11:00 am Plenary I: HLA Ligand Specificity and Lymphocyte Response

Regency Ballroom ABCD, Level Two


PROGRAMModerators: William Hildebrand PhD, D(ABHI), University of Oklahoma Health Science Center, Oklahoma, OK and Lee Ann Baxter-Lowe, PhD, D(ABHI), USC and CHLA, Los Angeles, CA, USA

9:30 am – 10:00 am Update on the Characterization of HLA Peptide Ligands Tony Purcell, PhD Monash University Clayton, Victoria, Australia

10:00 am – 10:30 am Update on the Characterization of HLA Ligands – Beyond Peptides

Erin Adams, PhD University of Chicago Chicago, IL, USA

10:30 am – 11:00 am HLA and Melanoma Vaccine Beatriz Carreno, PhD Washington University School of Medicine St. Louis, MO, USA


11:30 am – 1:30 pm User Group Meetings (page 31)

1:30 pm – 3:00 pm Abstract Session 1: Solid Organ Pre-Transplant

Assessments (page 56) Regency Ballroom ABCD, Level Two

Moderators: John Lunz, PhD, D(ABHI), Gift of Hope Organ & Tissue Donor Network, Itasca, IL, USA and Annette Jackson, PhD, D(ABHI), Johns Hopkins University, Baltimore, MD, USA

1:30 pm – 3:00 pm Abstract Session 2: Peptide Presentation and Cell Signaling (page 57)

Regency Ballroom E, Level Two

Moderators: George Vlad, PhD, D(ABHI), Columbia University Medical Center, New York, NY, USA and Marcelo Pando, PhD, Mayo Clinic, Phoenix, AZ, USA


PROGRAM1:30 pm – 3:00 pm Workshop 1: Round Table Discussions Gateway East, Level Eighteen Moderators: Donna Phelan, BA, CHS, MT, Barnes-

Jewish Hospital, St. Louis, MO, USA, Harriet Noreen, BS, CHS(ABHI), University of Minnesota Medical Center, Eagan, MN, USA, Brett Loehmann, CHS, CHT, CTBS, BS, Mid-South Transplant HLA Laboratory, Memphis, TN, USA, Lynn Klingman, MT(ASCP), CHS(ABHI), Baylor University Medical Center, Dallas, TX, USA, Sharon Adams, CHS(ABHI), MT(ASCP), National Institutes of Health, Bethesda, MD, USA, Lynn Kilheeney-Nichol, CHS, ABHI, UPMC Presbyterian Shadyside, Pittsburgh, PA, USA, Ruby Siegel, MS, CHS(ABHI), Stanford Blood Center, Palo Alto, CA, USA, Donna Lucas, MS, CHS, Johns Hopkins School of Medicine, Baltimore, MD, USA, Nancy Higgins, CHS, IU Health, Indianapolis, IN, USA, Karl Schillinger, CHS, Johns Hopkins University, Baltimore, MD, USA, Dr. Chang Liu, MD, PhD, D(ABHI), Washington University in St. Louis, St. Louis, MO, USA, Dr. Olga Timofeeva, PhD, D(ABHI), Temple University, Philadelphia, PA, USA, Anne Halpin, MSc, CHS, University of Alberta Hospital, Edmonton, AB, CAN and Gerald Morris, MD, PhD, University of California San Diego, La Jolla, CA, USA

3:00 pm – 3:30 pm Coffee Break in Exhibit Hall Grand Ballroom, Level Four

3:30 pm – 5:00 pm Special Abstract Session: Scholar Awards (page 58) Regency Ballroom ABCD, Level Two

Moderators: James Matthew, Northwestern University, Chicago, IL, USA and Paul Norman, PhD, Stanford University, Stanford, CA, USA

5:00 pm – 6:30 pm Poster Reception with Exhibit Hall Grand Ballroom, Level Four

6:30 pm – 8:30 pm ARB Inspectors and International Reception (Invitation Only)

Gateway Terrace, Level Eighteen


PROGRAMWEDNESDAY, SEPTEMBER 28, 2016

6:30 am – 8:00 am The Tulip Run – separate registration fee Transportation starts at 5:30 am from Hyatt Regency

St. Louis to Tower Grove.


8:30 am – 10:00 am Plenary II: Functional Elements within the HLA Genes and Proteins; Beyond the ARS


Moderators: Marcelo Fernandez Vina, PhD, Stanford University School of Medicine, Palo Alto, CA, USA and Amy Hahn, PhD, D(ABHI), Albany Medical College, Albany, NY, USA

8:30 am – 9:00 am The Contribution of STRs to the Genetic Architecture of Quantitative Human Traits

Melissa Gymrek, PhD Broad Institute of MIT and Harvard Cambridge, MA, USA

9:00 am – 9:30 am The Impact of the HLA Class II Membrane Proximal Region on Molecular Structure and Function

James Drake, PhD Albany Medical College Albany, NY, USA

9:30 am – 10:00 am Clonal Evolution in Acquired Aplastic Anemia Involving the HLA Locus

Timothy Olson, MD, PhD Children’s Hospital of Philadelphia Philadelphia, PA, USA


10:30 am – 12:30 pm Symposium I: NGS (Including Data Standards and Informatics)


Moderators: Dimitri Monos, PhD, University of Pennsylvania and Children’s Hospital of Philadelphia, Philadelphia, PA, USA and Martin Maiers, MS, National Marrow Donor Program, Minneapolis, MN, USA


PROGRAM10:30 am – 11:15 am Analysis of HLA Polymorphism by Next Generation

Sequencing: Strategies, Issues, and Clinical Applications

Henry Erlich, PhD Children’s Hospital & Research Institute Oakland Oakland, CA, USA

11:15 am – 11:55 am Large Scale Whole Gene and Long Range Sequencing of HLA Class I and Class II Genes: The landscape beyond ARS (Antigen Recognition Sites)

Nezih Cereb, MD Histogenetics Ossining, NY, USA

11:55 am – 12:15 pm ACES NIGH: Analysis, Collection, Exchange and Storage of NGS ImmunoGenomic and Histocompatibility Data

Steve Mack, PhD Children’s Hospital Oakland Research Institute Oakland, CA, USA

12:15 pm – 12:30 pm HLA Nomenclature in the NGS Era Steven GE Marsh, BSc, PhD, ARCS Anthony Nolan Research Institute London, United Kingdom

12:30 pm – 2:30 pm User Group Meetings (page 31)

2:30 pm – 4:00 pm Abstract Session 3: Solid Organ Post-Transplant Assessments (page 59)


Moderators: Peter Nickerson, MD, FRCPC, University of Manitoba, Winnipeg, MD, Canada and Daniel Ramon, PhD, D(ABHI), HCLD(ABB), University of Michigan, Ann Arbor, MI, USA

2:30 pm – 4:00 pm Abstract Session 4: New Applications of NGS and New Assays (page 61)


Moderators: Daniel Geraghty, PhD, Scisco Genetics, Inc., Seattle, WA, USA and Robert Bray, PhD, Emory University, Atlanta, GA, USA


PROGRAM2:30 pm – 4:00 pm Workshop 2: BMT: Permissible Mismatches in Donor

Selection; Chimerism and Engraftment Regency Ballroom F, Level Two

Moderators: Sharon Adams, CHS(ABHI), MT(ASCP), National Institutes of Health, Bethesda, MD, USA and Ruby Siegel, MS, CHS(ABHI), Stanford Blood Center, Palo Alto, CA, USA

2:30 pm – 3:15 pm Permissible Mismatches and Donor Selection Rizwan Romee, MD Washington University St. Louis, MO, USA

3:15 pm – 4:00 pm Chimerism Testing and Engraftment Monitoring Raja, Rajalingam, PhD, D(ABHI) University of California, San Francisco San Francisco, CA, USA

Denice Kong, BS University of California, San Francisco San Francisco, CA, USA 2:30 pm – 4:00 pm ASHI-AABB Joint Symposium:

Do You Really Know ABO? Parkview, Level Two

Moderator: Carol Pancoska, PhD, D(ABHI), Einstein Healthcare Network, Philadelphia, PA, USA

2:30 pm – 2:45 pm New Standards and Regulations and Why We Need Them

Patricia Kopko, MD University of California San Diego, CA, USA

2:45 pm – 3:00 pm Current Status of ABO Testing and Transplant Applications

Paul Warner, PhD Bloodworks Northwest Seattle, WA, USA


PROGRAM3:00 pm – 3:45 pm A New Generation in ABO Antibody Diagnostics Lori West, MD University of Alberta Edmonton, Alberta, Canada

3:45 pm – 4:00 pm Q&A

4:15 pm – 5:45 pm Workshop 3: Becoming A Citizen Of The NGS World: All Considered


Moderators: Harriet Noreen, BS, CHS(ABHI), University of Minnesota Medical Center, Eagan, MN, USA and Lynn Klingman, MT(ASCP), CHS(ABHI), Baylor University Medical Center, Dallas, TX, USA

4:15 pm – 4:35 pm Making the Most of High Throughput Medhat Askar, MD, PhD, D(ABHI) Baylor University Medical Center Dallas, TX

4:35 pm – 4:55 pm Tailoring NGS for Smaller Volume Labs Lee Ann Baxter-Lowe, PhD, D(ABHI) Childrens Hospital of Los Angeles Los Angeles, CA, USA

4:55 pm – 5:10 pm Using Quality Metrics Lee Ann Baxter-Lowe, PhD, D(ABHI) Childrens Hospital of Los Angeles Los Angeles, CA, USA

5:10 pm – 5:25 pm Validating NGS Methods Medhat Askar, MD, PhD, D(ABHI) Baylor University Medical Center Dallas, TX

5:25 pm – 5:45 pm Q&A 4:15 pm – 5:45 pm Workshop 4: Abstract Case Studies for BMT and Solid

Organ Transplantation (page 62) Regency Ballroom F, Level Two

Moderators: Nancy Higgins, CHS, IU Health, Indianapolis, IN, USA


PROGRAM5:45 pm – 6:45 pm Directors’ Forum Regency Ballroom E

5:45 pm – 6:45 pm Technologists’ Forum Regency Ballroom F

THURSDAY, SEPTEMBER 29, 2016

7:15 am – 8:15 am Women in Transplantation Breakfast Regency Ballroom E, Level Two


8:30 am – 10:00 am Plenary III: Scientific Award Session Regency Ballroom ABCD, Level Two

Moderators: Anat Tambur, DMD, PhD, Northwestern University, Chicago, IL, USA and Michael Gautreaux, PhD, Wake Forest School of Medicine, Clemmons, NC, USA

8:30 am – 9:15 am Distinguished Scientists Awardee: HLA Amino Acid Polymorphisms and Kidney Allograft Outcomes

Malek Kamoun, MD, PhD University of Pennsylvania Philadelphia, PA, USA

9:15 am – 10:00 am Paul I. Terasaki Clinical Science Awardee: Back the the Future: HLA Matching to Improve Outcomes

Peter Nickerson, MD, FRCPC University of Manitoba Winnipeg, Manitoba, Canada


10:30 am – 12:30 pm Symposium II: Targeting B, Plasma Cells and Complement for the Treatment of Allograft Rejections


Moderators: Howie Gebel, PhD, Emory University Hospital, Atlanta, GA, USA and Elaine Reed, PhD, D(ABHI), University of California Los Angeles, Los Angeles, CA, USA


PROGRAM10:30 am – 11:00 am B Cell Memory Subsets Michael Cancro, PhD University of Pennsylvania Philadelphia, PA, USA

11:00 am – 11:30 am Non Human Primate Trials Targeting B Cells and Complement

Ali Naji, MD, PhD University of Pennsylvania Philadelphia, PA, USA

11:30 am – 12:00 pm CAR T cells Targeting Plasma Cells in Clinical Trials of Multiple Myeloma

Michael Milone, MD, PhD University of Pennsylvania Philadelphia, PA, USA

12:00 pm – 12:30 pm Autoimmunity in Transplantation and its Clinical Relevance

Marie Josee Hebert, MD, FRCPC CR CHUM Montreal, QC, Canada

12:30 pm – 2:30 pm User Group Meetings (page 31)

2:30 pm – 4:00 pm Abstract Session 5: New Insights of NGS (page 64) Regency Ballroom ABCD, Level Two

Moderators: Medhat Askar, MD, PhD, FRCPath, Baylor University Medical Center, Dallas, TX, USA and Rajalingham Raja, PhD, University of California San Francisco, San Francisco, CA, USA

2:30 pm – 4:00 pm Abstract Session 6: Hematopoietic Stem Cell Transplantation (page 65)


Moderators: Victoria Turner, PhD, D(ABHI), Laboratory Consultants of Florida, Memphis, TN, USA and Faisal Khan, PhD, D(ABHI), University of Calgary, Calgary, AB, Canada


PROGRAM2:30 pm – 4:00 pm Workshop 5: Report on the New KAS – Its Impact on

Allocation, Graft Survival and Laboratory Practice Regency Ballroom F, Level Two

Moderators: Donna Phelan, BA, CHS, MT, Barnes-Jewish Hospital, St. Louis, MO, USA and Karl Schillinger, MS, CHS, Johns Hopkins University, Baltimore, MD, USA 2:30 pm – 3:00 pm Impact on Laboratory Practice (UNOS perspective) Robert Bray, PhD Emory University Atlanta, GA, USA

3:00 pm – 3:30 pm Antibody Identification and Entry in UNET Nicole Turgeon, MD Emory University Atlanta, GA, USA

3:30 pm – 4:00 pm The Effect of KAS on Laboratory Practices Lynn Klingman, MT(ASCP),CHS(ABHI) Baylor University Medical Center Dallas, TX, USA

Karl Schillinger, CHS, (ABHI) Johns Hopkins Immunogenetics Laboratory Baltimore, MD, USA

4:00 pm – 4:30 pm Coffee Break Regency Ballroom Prefunction, Level Two

4:30 pm – 6:00 pm Symposium III: Awards, Business Meeting & Updates Regency Ballroom ABCD, Level Two

6:00 pm – 7:00 pm Networking Poster Reception Grand Ballroom FGH, Level Four


PROGRAMFRIDAY, SEPTEMBER 30, 2016


8:30 am – 10:00 am Plenary IV: HSCT: Haplo vs. URD Regency Ballroom ABCD, Level Two

Moderators: Maria Bettinotti, PhD, Johns Hopkins University, Baltimore, MD, USA and John Schmitz, PhD, UNC Chapel Hill, Chapel Hill, NC, USA

8:30 am – 9:00 am Immunogenicity of HLA mismatches and Criteria for MUD Prioritization

Marcelo Fernandez Vina, PhD Stanford Blood Center Palo Alto, CA, USA

9:00 am – 9:30 am Development of Protocols and Transplant Outcomes with Haploidentical Donors

Stefan Ciurea, MD MD Anderson Cancer Center Houston, TX, USA

9:30 am – 10:00 am Global Application of Transplants with Haploidentical Donors Criteria for Donor Selection

Ephraim Fuchs, MD Sidney Kimmel Comprehensive Cancer Center at Johns

Hopkins Baltimore, MD, USA

10:00 am – 10:30 am Coffee Break Regency Ballroom Prefunction, Level Two

10:30 am – 12:00 pm Plenary V: Take a Deep Breath – Optimizing Outcomes in Lung Transplantation


Moderators: Thalachallour Mohanakumar, PhD, D(ABHI), St. Joseph’s Hospital and Medical Center, Phoenix, AZ, USA

10:30 am – 11:00 am Desensitization in Lung Transplantation Christopher Ensor, PharmD, BCPS University of Pittsburgh Pittsburgh, PA, USA


PROGRAM11:00 am – 11:30 am Ethics and Allocation in Lung Transplantation Stuart Sweet, MD, PhD Washington University St. Louis, MO, USA

11:30 am – 12:00 pm Ex Vivo Lung Perfusion – Opportunities to Expand the Donor Pool

Marcelo Cypel, MD, MSc Toronto General Hospital Toronto, ON, CAN


ABSTRACTSTuesday, September 27, 2016

1:30 pm – 3:00 pmAbstract Session 1: Solid Organ Pre-transplant Assessments

OR1MAPPING ANTI-HLA ANTIBODY EPITOPES BY PAINTING THE HLA BLUECurtis McMurtrey1, Virginie Sjoelund2, Rico Buchli3, William Hildebrand1, 1University of Oklahoma Health Sciences Center, Microbiology and Immunology, Oklahoma City, OK, 2University of Oklahoma Health Sciences Center, OUHSC Core Labs, Oklahoma City, OK, 3Pure Protein LLC, Austin, TX, United States.

OR2VARIABLE EXPRESSION OF HLA-C IMPACTS T VERSUS B CELL CROSSMATCH OUTCOMESDonna P. Lucas, Renato M. Vega, Annette M. Jackson, The Johns Hopkins School of Medicine, Medicine, Baltimore, MD.

OR3THE STRENGTH OF POSITIVE LINEAR CORRELATIONS BETWEEN DONOR-SPECIFIC HLA ANTIBODIES AND FLOW-CYTOMETRY CROSSMATCH IS LOCUS-DEPENDENTKelly Cunniffe, Jack Lai, Raja Rajalingam, Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA.

OR4POSSIBLE PARAMETERS FOR DETERMINATION OF UNACCEPTABLE DP ANTIGENS FOR TRANSPLANTATIONDeborah O. Crowe1, Christina M. Bishop2, 1DCI Laboratory, Transplant Immunology, Nashville, TN, 2DCI Laboratory, Transplant Laboratory, Knoxville, TN.

OR5CAN YOU PREDICT ACCURATE CROSSMATCH RESULTS WITH HLA-DP DSA?Renato M. Vega, Annette Jackson, Johns Hopkins University, Medicine, Baltimore, MD.

OR6THE POSITIVE VIRTUAL CROSSMATCH. CORRELATION BETWEEN HLA DSA AND FLOW CYTOMETRY CROSSMATCH RESULTS.


ABSTRACTSJuliana Montagner1, Heloisa Tarasconi1, Joao Wurdig1, Elizete Keitel2, Robert Liwski3, Jorge Neumann1, 1Laboratory of Transplantation Immunology, Santa Casa Hospital, Porto Alegre, BRAZIL, 2Kidney Transplantation Unit, Santa Casa Hospital, Porto Alegre, BRAZIL, 3Dalhousie University, Halifax, NS, CANADA.

OR7THE UCLA VIRTUAL CROSSMATCH EXCHANGEArlene F. Locke, Qiuheng Zhang, Elaine F. Reed, David W. Gjertson, UCLA Immunogenetics Center, Pathology and Lab Medicine, Los Angeles, CA.

Tuesday, September 27, 20161:30 pm – 3:00 pm

Abstract Session 2: Peptide Presentation and Cell Signaling

OR8QUANTITATIVE ASSESSMENT OF HLA CLASS I PRESENTATION IN ICT-107, A DENDRITIC CELL-BASED IMMUNOTHERAPYSaghar Kaabinejadian1, Rico Buchli2, Steven J. Swanson3, Anthony J. Gringeri3, Jon A. Weidanz4, William H. Hildebrand1, 1University of Oklahoma Health Sciences Center, Microbiology and Immunology, Oklahoma, OK, 2Pure MHC LLC., Oklahoma City, OK, 3ImmunoCellular Therapeutics, Ltd., Calabasas, CA, 4Texas Tech University Health Siences Center, Immunotherapeutics and Biotechnology, Abilene, TX.

OR9A SINGLE AMINO ACID RESIDUE WITHIN THE HLA-B*35 HEAVY CHAIN DETERMINES THE IMMUNE DOMINANCE OF PEPTIDES DURING HCMV INFECTIONWiebke C. Abels, Heike Kunze-Schumacher, Trishna Manandhar, Rainer Blasczyk, Christina Bade-Doeding, Hannover Medical School, Transfusion Medicine, Hannover, GERMANY.

OR10TUMOR ANTIGENS PRESENTED BY THE HLA-A*02:01 OF BREAST CANCER PATIENT-DERIVED XENOGRAFTSChrista DeVette1, Saghar Kaabinejadian1, Maihi Fujita2, William Hildebrand1, Alana Welm2, 1University of Oklahoma Health Sciences Center, Microbiology & Immunology, Oklahoma City, OK, 2Huntsman Cancer Institute, Oncological Sciences, Salt Lake City, UT.


ABSTRACTSOR11HLA LINKED PHARMACOGENOMICS: CARBAMAZEPINE ALTERATION OF THE HLA-A*31:01 PEPTIDE BINDING GROOVEHeike Kunze-Schumacher, Lea Beth, Alexander A. Celik, Trevor Huyton, Rainer Blasczyk, Christina Bade-Doeding, Hannover Medical School, Transfusion Medicine, Hannover, GERMANY.

OR12INTEGRIN Β1 PARTNERS WITH HLA CLASS II MOLECULES TO MEDIATE ENDOTHELIAL CELL (EC) FUNCTIONSFang Li, Elaine F. Reed, UCLA, Pathology and Lab Medicine, Los Angeles, CA.

OR13MITOGEN-ACTIVATED PROTEIN KINASE P38 AND C-JUN NH2-TERMINAL KINASE REGULATE HLA CLASS II ANTIBODY-STIMULATED ACTIVATION OF HUMAN AORTIC ENDOTHELIAL CELLSYi-Ping Jin, Elaine F. Reed, University of California Los Angeles, Pathology and Laboratory Medicine, Los Angeles, CA.

OR14HLA CLASS I ANTIBODIES INCREASED WNT/Β-CATENIN PATHWAY COMPONENT PROTEIN LYMPHOID-BINDING FACTOR 1 (LEF1) IN ENDOTHELIAL CELLS IN FOXO1 PROTEIN DEPENDENT FASHION Katherine Twombley, Minoo Kavarana, Andrew Savage, Sheree Waslaske, Omar Moussa, Medical University of South Carolina, Charleston, SC.

Tuesday, September 27, 20163:30 PM - 5:00 PM

Special Abstract Session: Scholar Awards

OR15NEW NGS HLA TYPING BY TARGETED ENRICHMENT PROCEDURE, CAPTURE METHODHidetoshi Inoko1, Yuko Okudaira1, Anri Masuya1, Atsushi Tajima2, Kazuyoshi Hosomichi2, 1GenoDive Pharma Inc., Honatusgi, Kanagawa, JAPAN, 2Kanazawa University, Kanazawa, Ishikawa, JAPAN.


ABSTRACTSOR16ANTIBODIES TO DONOR HUMAN LEUKOCYTE ANTIGEN INDUCE EXOSOMES CONTAINING TISSUE-RESTRICTED SELF-ANTIGENS IN ACUTE AND CHRONIC REJECTIONMuthukumar Gunasekaran1, Monal Sharma1, Ramsey Hachem2, Rajat Walia1, Surendra Shenoy3, Michael Smith1, Ross Bremner1, Thalachallour Mohanakumar1, 1St. Joseph’s Hospital & Medical Center, Norton Thoracic Institute, Phoenix, AZ, 2Washington University School of Medicine, Medicine, St. Louis, MO, 3Washington University School of Medicine, Surgery, St. Louis, MO.

OR17HLA-B INTRON DERIVED MICRORNA REGULATES THE POSTTRANSCRIPTIONAL EXPRESSION OF THE HEAVY CHAIN OF THE IMMUNOGLOBULIN ANilesh S. Chitnis1, Peter M. Clark1, Bradley Johnson2, Catherine Stolle1, Malek Kamoun2, Dimitri Monos1,2, 1Children’s Hospital of Philadelphia, Philadelphia, PA, 2University of Pennsylvania, Philadelphia, PA.

OR18FULL GENE SEQUENCING REVEALS VERY LIMITED VARIATION IN THE REGIONS OUTSIDE OF THE ANTIGEN RECOGNITION DOMAINS (ARD) OF 360 UNRELATED HEMATOPOIETIC STEM CELL TRANSPLANT DONOR-RECIPIENT PAIRS MATCHED FOR 10/10 AT HIGH RESOLUTIONCarolyn Hurley1, Lihua Hou2, Cynthia Vierra-Green3, Colleen Brady3, Mike Haagenson3, Stephen Spellman3, 1Georgetown University Hospital, Immunobiology Research, Washington, DC, 2Georgetown University School of Medicine, Immunobiology Research, Rockville, MD, 3CIBMTR, Immunobiology Research, Minneapolis, MN.

Wednesday, September 28, 20162:30 PM - 4:00 PM

Abstract Session 3: Solid Organ Post-transplant Assessments

OR19QUANTIFICATION OF PERIPHERAL B- CELL SUBSETS IN ACUTE ALLOGRAFT REJECTION IN RECIPIENTS WITH RENAL TRANSPLANTATIONRavi Dhital1, Mukut Minz1, Ranjana W. Minz2, Ashish Sharma1, Ritambhara Nada3, Sarbpreet Singh1, Deepesh Kenwar1, 1Post Graduate Institution of Medical Education and Research, Transplant Surgery, Chandigarh, INDIA, 2Post Graduate Institution of Medical Education and Research, Immunopathology, Chandigarh, INDIA, 3Post Graduate Institution of Medical Education and Research, Histopathology, Chandigarh, INDIA.


ABSTRACTSOR20CHARACTERIZATION OF DE NOVO DONOR-SPECIFIC HLA ANTIBODIES AND CORRELATION WITH C4D+ ANTIBODY-MEDIATED REJECTION OF KIDNEY TRANSPLANTSDessislava Kopchaliiska1, Owen Buenaventura1, Stephen Tomlanovich2, Ivan Balazs3, Raja Rajalingam1, 1Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA, 2Kidney Transplant Service, Department of Medicine, University of California San Francisco, San Francisco, CA, 3Immucor Transplant Diagnostics Inc, Stamford, CT.

OR21CLINICAL SIGNIFICANCE OF C3D BINDING DONOR SPECIFIC ANTI-HLA ANTIBODIES DETECTED BY SINGLE ANTIGEN BEADS AFTER KIDNEY TRANSPLANTATIONPaula Steller1, Ronald P. Pelletier2, Ivan Balazs3, Nicholas DiPaola4, Patrick W. Adams1, Alejandro Diez5, Mitchell Henry2, 1The Ohio State University Wexner Medical Center, Tissue Typing, Columbus, OH, 2The Ohio State University Wexner Medical Center, Surgery/Transplant, Columbus, OH, 3Immucor, Inc., Discovery Research, Stamford, CT, 4Houston Methodist Hospital, Houston, TX, 5The Ohio State University Wexner Medical Center, Nephrology, Columbus, OH.

OR22IDENTIFICATION AND VALIDATION OF NON-HLA ANTIBODIES IN ALLOGRAFT RECIPIENTSCarrie Butler1, Ping Rao1, Ning Jiang2, Qiuheng Zhang3, Martin Cadeiras4, Mario Deng4, Meghan Pearl5, Eileen Tsai5, Ivan Balazs2, Elaine F. Reed3,1, 1UCLA, Pathology, Los Angeles, CA, 2Immucor, Inc., Stamford, CT, 3UCLA, Immunogenetics Center, Los Angeles, CA, 4UCLA, Cardiology, Los Angeles, CA, 5UCLA, Pediatric Nephrology, Los Angeles, CA.

OR23ANTIBODIES TO MICA, AT1R AND ENDOTHELIAL CELLS OCCUR INDEPENDENTLY IN TRANSPLANT PATIENTS AND IN SERANicole M. Valenzuela, Jennifer Zhang, Michelle Hickey, Elaine F. Reed, UCLA, UCLA Immunogenetics Center, Los Angeles, CA.

OR24RECIPIENT FCGR3A-158V HOMOZYGOUS GENOTYPE IS ASSOCIATED WITH AN INCREASED RISK OF CHRONIC LUNG ALLOGRAFT DYSFUNCTIONHaibo Sun1, John R. Greenland2,3, Dessislava Kopchaliiska1, David D. Gae1, Jonathan P. Singer2, Jeffrey A. Golden2, Steven R. Hays2, Daniel Calabrese2, Jasleen Kukreja4, Raja Rajalingam1, 1Immunogenetics and Transplantation Laboratory, Department of


ABSTRACTSSurgery, University of California San Francisco, San Francisco, CA, 2Department of Medicine, University of California San Francisco, San Francisco, CA, 3Medical Service, San Francisco VA Medical Center, San Francisco, CA, 4Division of Cardiothoracic Surgery, Department of Surgery, University of California San Francisco, San Francisco, CA.

OR25IMMUNOTHERAPY INDUCED RAPID REDUCTION OF PREFORMED DSA IN HIGHLY SENSITIZED RECIPIENTS OF VISCERAL TRANSPLANTATIONAiwen Zhang1, Ajai Khanna2, Yuchu Sun3, Derek Good1, Kathy Zimmerman1, Gabriela Hayward-Lara1, Karen Seifarth1, Galal El-Gazzaz4, Masato Fujiki2, Koji Hashimoto2, Guilherme Costa5, Kareem Abu-Elmagd2, 1Cleveland Clinic, Allogen Laboratories, Cleveland, OH, 2Cleveland Clinic, DIgestive Disease Institute, Cleveland, OH, 3Avalon Medical University, Youngstown, OH, 4Cleveland Clinic, Cleveland, OH, 5Cleveland Clinic, Transplantation Center, Cleveland, OH.


Abstract Session 4: New Applications of NGS and New Assays

OR26TAKING THE HLA-SPECIFIC MEMORY B CELL ELISPOT TO THE NEXT LEVEL: ASSAYING THE FULL DONOR HLA REPERTOIREGonca E. Karahan, Yvonne de Vaal, Juliette Krop, Dave Roelen, Frans Claas, Sebastiaan Heidt, Leiden University Medical Center, Immunohematology and Blood Transfusion, Leiden, NETHERLANDS.

OR27MICRORNAS IN SERUM AS NONINVASIVE BIOMARKERS OF ACUTE CELLULAR REJECTION IN HEART ALLOGRAFT PATIENTSSophey Y. Ho1, Chih-Chao Chang1, Zheng Xu1, Eric K. Ho1, Charles C. Marboe1, Maryjane A. Farr2, Nicole Suciu-Foca1, Elena-Rodica M. Vasilescu1, George Vlad1, 1Columbia University, Pathology and Cell Biology, New York, NY, 2Columbia University, Medicine, New York, NY.

OR28ADVANCES IN MOLECULAR TYPING OF ABO/RHD AND CCR5Δ32 MUTATIONYuxin Yin1, James Lan2, Ping Takemura1, Gail Flickinger3, Edgar Schreiber4, Jar-How Lee5, Elaine F. Reed1, Qiuheng Zhang1, 1UCLA Immunogenetics Center, Pathology & Laboratory Medicine, LA, CA, 2University of British Columbia, Clinician Investigator


ABSTRACTSProgram, Vancouver, BC, CANADA, 3National Marrow Donor Program, Minneapolis, MN, 4Genetic Systems Applications, Thermo Fisher Scientific, South San Francisco, CA, 5One Lambda, Thermo Fisher Scientific, Canoga Park, CA.

OR29PREDICTION OF ABO RH SEROTYPES BY MOLECULAR TYPING OF ABO RH GENES IS HIGHLY CONCORDANT WITH SEROLOGICAL TYPING: EXPERIENCE WITH TYPING 1000000 SAMPLESNezih Cereb1, Sang Yeol Seo1, Amaralingeswara Rao1, Gail Flickinger2, Jangyoung Kwon1, JeongOk Jeon1, DongYong Kim1, HwaRan Kim1, Romy Kronstein3, Torsten Tonn3, Soo Young Yang1, 1Histogenetics, Ossining, NY, 2National Marrow Donor Program/Be The Match, Minneapolis, MN, 3Technical University Dresden, Dresden, GERMANY.

OR30DUAL REDUNDANT REFERENCE SEQUENCING (DR2S): A COMPUTATIONAL APPROACH FOR PHASE-DEFINED FULL-LENGTH HLA-GENE CHARACTERIZATIONGerhard Schöfl1, Kathrin Lang1, Alexander H. Schmidt2, Vinzenz Lange1, 1DKMS Life Science Lab, Dresden, GERMANY, 2DKMS, Tübingen, GERMANY.

OR31THE ECONOMICS OF NEXT GENERATION SEQUENCINGPeter Meintjes1, Nora Nagy2, Efi Melista2, Tim Hague2, Krisztina Rigó2, 1Omixon Inc., Cambridge, MA, 2Omixon Biocomputing Kft., Budapest, HUNGARY.

OR32POTENTIAL ROLE OF LOCI IN LINKAGE DISEQUILIBRIUM WITH DRB1 IN THE PATHOGENESIS OF RHEUMATOID ARTHRITISKirsten M. Anderson, Christina Roark, Michael Aubrey, Brian Freed, University of Colorado Denver, Immunology, Aurora, CO.


Workshop 4: Abstract Case Studies for BMT and Solid Organ

OR47ALLELE-SPECIFIC ANTIBODY TO HLA-DQ ALPHA CHAIN IN A CASE OF CHRONIC ANTIBODY-MEDIATED REJECTIONQingyong Xu1, Elly Johnson2, Twyla Pearce2, Robert Liwski3, 1London Health Science


ABSTRACTScenter, London, ON, CANADA, 2Saskatoon Health Region, Saskatoon, SK, CANADA, 3Dalhousie University, halifax, NS, CANADA.

OR48RESOLUTION OF CONFLICTING HLA ASSIGNMENT DUE TO LOSS OF HETEROZYGOSITY IN THE HLA REGION BY NGS TYPING Eszter Lazar-Molnar1, Erica F. Andersen1, Tracie Profaizer2, Ann Pole3, Michelle R. Taylor3, Julio C. Delgado1, 1University of Utah School of Medicine, Pathology, Salt Lake City, UT, 2ARUP Laboratories, Salt Lake City, UT, 3University of Utah Healthcare, Histocompatibility & Immunogenetics Laboratory, Salt Lake City, UT.

OR49DETECTING CHIMERISM FOLLOWING LUNG TRANSPLANT WITH GRAFT VS. HOST DISEASELauren Clark, Nick Woolley, Angela Hoover, Amit D. Parulekar, Goutham Dronavalli, Ronald Kerman, Peter Jindra, Baylor College of Medicine, Surgery, Houston, TX.

OR50POTENTIAL NEW MOLECULAR MARKERS TO SELECT DONORS FOR BONE MARROW/HEMATOPOEITIC STEM CELL TRANSPLANTATIONGopal Patel1, Ina Kurbegovic-Skaljic1, Sylvia Piggott1, Antonio Jimenez2, Rachel Mitchell2, Piyanuch Kongtim2, Deborah Katz2, Sunita Nathan2, John Maciejewski2, Gorgun Akpek2, Siva Kanangat1, 1Rush University Medical Center, Pathology, Chicago, IL, 2Rush University Medical Center, Hematology and Cell therapy, Chicago, IL.

OR51NOT ALL STR MARKERS ARE CREATED EQUAL: LOSS OF HETEROZYGOSITY RENDERS INFORMATIVE MARKERS TO NONINFORMATIVE IN CHIMERISM TESTINGXiaohai (Sam) Zhang, Qi Wang, Nancy Reinsmoen, Cedars-Sinai Health Systems, Los Angeles, CA.

OR52HLA TYPING FROM A DECEASED DONOR WHO RECEIVED HEMATOPOIETIC STEM CELL TRANSPLANTQingyong Xu, Steve Leckie, London Health Science Center, London, ON, CANADA.


ABSTRACTSOR53THREE CASE REPORTS: NGS-BASED HIGH RESOLUTION HLA TYPING PERMITS BETTER ASSESSMENT OF DONOR-RECIPIENT COMPATIBILITY IN SOLID ORGAN TRANSPLANTYanping Huang, Allison Gasiewski, Hilary Mehler, Steven Heron, Deborah Ferriola, Dimitri Monos, Children’s Hospital of Philadelphia, Philadelphia, PA.

Thursday, September 29, 20162:30 PM - 4:00 PM

Abstract Session 5: New Insights of NGS

OR33ALLELIC DIVERSITY AND HAPLOTYPE STRUCTURE OF HLA-DPB1 BASED ON FULL-LENGTH CHARACTERIZATION OF MOST CWD ALLELESGerhard Schöfl1, Kathrin Lang1, Marie Günther1, Grit Schober1, Carolin Massalski1, Alexander H. Schmidt2, Vinzenz Lange1, 1DKMS Life Science Lab, Dresden, GERMANY, 2DKMS, Dresden, GERMANY.

OR34EXAMINATION OF HLA DP INTON/EXON VARIATION IDENTIFIES TWO DPB1 EVOLUTIONARY GROUPS INCLUDING INTRON 2 STR VARIANTS THAT MAY REGULATE DPB1 EXPRESSION LEVELSKonstantinos Barsakis, Farbod Babrzadeh, Anjo Chi, Michael N. Mindrinos, Marcelo A. Fernandez Vinã, Stanford Blood Center, School of Medicine, Palo Alto, CA.

OR35WHOLE GENE CLASS I SEQUENCING IN A LARGE SAMPLE VOLUME REVEALS EXTENSIVE VARIATION IN INTRONIC REGIONSNezih Cereb, HwaRan Kim, Jaejun Ryu, Eunsil Kim, Vikas Rai, Seho Choi, Hyeon Jin Park, Jangyoung Kwon, Soo Young Yang, Histogenetics, Ossining, NY.

OR36DEFINING KIR AND HLA CLASS I ALLELES AT HIGHEST RESOLUTION USING NEXT GENERATION SEQUENCINGNeda Nemat-Gorgani1, Wesley M. Marin2, Jill A. Hollenbach2, Jorge Oksenberg2, Paul J. Norman1, Peter Parham1, 1Stanford University, Structural Biology, Stanford, CA, 2UCSF, Neurology, San Fransisco, CA.


ABSTRACTSOR37HAPLOTYPE ANALYSES OF CLASSICAL HLA GENES FROM QUARTET FAMILIESKazutoyo Osoegawa1, Lisa Creary2, Kalyan Mallempati1, Sridevi Gangavarapu1, Marcelo A. Fernandez Vina2, 1Stanford Blood Center, Palo Alto, CA, 2Stanford University, Palo Alto, CA.

OR38WHOLE GENE HLA CLASS I GENOTYPING WITH THE MINION NANOPORE SEQUENCERKathrin Lang1, Philipp Quenzel1, Bianca Schöne1, Gerhard Schöfl1, Alexander H. Schmidt2, Vinzenz Lange1, 1DKMS Life Science Lab, Dresden, GERMANY, 2DKMS, Tübingen, GERMANY.

OR39THE POWER OF SINGLE MOLECULE SEQUENCING IN HLA DIAGNOSTICSMathijs Groeneweg, Christina E. Voorter, Ben M. Matern, Thuur W. Slangen, Marcel G. Tilanus, Maastricht University Medical Center, Transplantation Immunology, Maastricht, NETHERLANDS.

Thursday, September 29, 20162:30 PM - 4:00 PM

Abstract Session 6: Hematopoietic Stem Cell Transplantation

OR40HIGH CELL FREE DNA (CFDNA) RECIPIENT CHIMERISM DURING EARLY POST-TRANSPLANT PERIOD PREDICTS CLINICALLY SIGNIFICANT GRAFT VERSUS HOST DISEASEGaurav Tripathi1, Poonam D. Khan1, Rehan M. Faridi1, Gary Sinclair2, Noureddine Berka2, Jan Storek3, Faisal M. Khan4, 1University of Calgary, Department of Pathology and Laboratory Medicine, Calgary, AB, CANADA, 2University of Calgary, Pathology and Laboratory Medicine, Calgary Laboratory Services, Calgary, AB, CANADA, 3University of Calgary, Blood and Bone Marrow Transplant Program, Tom Baker Cancer Center, Calgary, AB, CANADA, 4University of Calgary, Pathology and Laboratory Medicine, Pediatrics, Calgary Laboratory Services, Calgary, AB, CANADA.

OR41TRANSCRIPTOME ANALYSIS OF IMMUNITY RELATED GENES AS EARLY PREDICTOR OF GRAFT VERSUS HOST DISEASE


ABSTRACTSPoonam Dharmani-Khan1, Rehan M. Faridi1, Ariz Akhter1, Noureddine Berka1, Jan Storek1, Faisal M. Khan2, 1University of Calgary, Medicine, Calgary, AB, CANADA, 2University of Calgary, Pathology and Laboratory Medicine, Calgary, AB, CANADA.

OR42IMPUTATION OF DPB1 FOR DONOR SELECTION IN THE MAJOR US RACE GROUPSMichael S. Halagan1, Loren Gragert2, Carolyn K. Hurley3, Carly Masaberg3, Martin Maiers1, 1National Marrow Donor Program, Bioinformatics Research, Minneapolis, MN, 2Tulane University School of Medicine, Department of Pathology and Laboratory Medicine, New Orleans, LA, 3Georgetown University Medical Center, Department of Oncology, Washington, DC.

OR43FREQUENCY OF HLA DPA1 AND DPB1 MISMATCHING IN A POPULATION OF 1199 PAIRS OF PRESUMED HLA IDENTICAL SIBLING TRANSPLANT PAIRSCynthia Vierra-Green1, MIke Haagenson1, Mary Eapen2, Colleen Brady1, Nezih Cereb3, Marcelo Fernandez-Vina4, Bronwen Shaw5, Katharina Fleischhauer6, Stephen Spellman1, 1CIBMTR, Immunobiology Research, Minneapolis, MN, 2Medical College of Wisconsin, CIBMTR - Milwaukee, Immunobiology Research, Millwaukee, WI, 3Histogenetics, Immunobiology Research, Ossining, NY, 4Stanford Healthcare, Immunobiology Research, Standford, CA, 5Medical College of Wisconsin, CIBMTR - Milwaukee, Immunobiology Research, Milwaukee, WI, 6Universitatsklinikum Essen KMT, Immunobiology Research, Essen, D-45122, GERMANY.

OR44ASSESSING THE DYNAMICS BETWEEN TWO CORD BLOOD UNITS IN ADULT DOUBLE CORD TRANSPLANTATIONMatthew Cusick, Olga Frakfurt, Yelena Barabanova, Anat Tambur, Northwestern, Surgery, Chicago, IL.

OR45RNA SEQUENCING OF TWO LYMPHOBLASTOID CELL LINES REVEALS NOVEL MICRORNA TRANSCRIPTS OF THE MHCPeter M. Clark1, Nilesh Chitnis1, Brad Johnson2, Catherine Stolle1, Malek Kamoun2, Dimitri Monos1,2, 1The Children’s Hospital of Philadelphia, Pathology & Laboratory Medicine, Philadelphia, PA, 2University of Pennsylvania Perelman School of Medicine, Pathology & Laboratory Medicine, Philadelphia, PA.

OR46DETECTING LOW LEVEL CLONAL SOMATIC MUTATION IN HLA GENES USING NEXT-GENERATION SEQUENCING IN THE


ABSTRACTSPRESENCE OF APLASTIC ANEMIAJamie L. Duke1, Daria V. Babushok2,3, Hongbo M. Xie4, Hilary Mehler1, Yanping Huang1, Timothy S. Olson2, Dimitri S. Monos1,5, 1The Children’s Hospital of Philadelphia, Department of Pathology and Laboratory Medicine, Philadelphia, PA, 2The Children’s Hospital of Philadelphia, Department of Pediatrics, Division of Hematology, Comprehensive Bone Marrow Failure Center, Philadelphia, PA, 3Hospital of the University of Pennsylvania, Division of Hematology-Oncology, Department of Medicine, Philadelphia, PA, 4The Children’s Hospital of Philadelphia, Department of Biomedical and Health Informatics, Philadelphia, PA, 5University of Pennsylvania, Perelman School of Medicine, Department of Pathology and Laboratory Medicine, Philadelphia, PA.

Tuesday, September 26, 20165:30 pm – 7:00 pm

Poster Session

P001ON THE OCCURRENCE OF NON-CWD ALLELES IN THE GERMAN DKMS DONOR DATABASEDaniel M. Baier, Jan A. Hofmann, Julia Pingel, Alexander H. Schmidt, Jürgen Sauter, DKMS, Tübingen, GERMANY.

P002THE CONSISTENT INCONSISTENCY IN HLA ANTIBODY TESTING PRACTICES: A COLLABORATION WITH THE BANFF WORKGROUPD Dadhania1, A Jackson2, P Campbell3, L Bow4, K Almeshari5, C Schinstock6, L Cornell6, S Bagnasco2, E Kraus7, E Cozzi8, M Askar9, 1Weill Cornell Medicine -NYP, Transplantation Medicine, New York, NY, 2Johns Hopkins Medicine, Transplantation Medicine, Baltimore, MD, 3University of Alberta, Transplantation Medicine, Edmonton, AB, CANADA, 4Yale University, Transplantation Medicine, New Haven, CT, 5King Faisal Specialist Hospital and Research Center, Transplantation Medicine, Riyadh, SAUDI ARABIA, 6Mayo Clinic, Transplantation Medicine, Rochester, MN, 7Johns Hopkins Medicine, Transplantation Medicine, Baltimore, MD, 8University of Padua, Transplantation Medicine, Padova, ITALY, 9Baylor University, Transplantation Medicine, Dallas, TX.

P003IMPACT OF THE NEW KIDNEY ALLOCATION SYSTEM ON THE TRANSPLANT RATES OF HIGHLY SENSITIZED PATIENTS. A SINGLE CENTER EXPERIENCEShirley Chan, Christina Savchik, Peter Masiakos, Maria Ajaimy, Juan Rocca, Enver Akalin, Adriana Colovai, Montefiore Medical Center, Bronx, NY.


ABSTRACTSP004THE CHALLENGE OF VIRTUAL CROSSMATCHING IN THE PRESENCE OF WEAK DONOR SPECIFIC ANTIBODIESIdoia Gimferrer, Rhonda Loken, Paul Warner, BloodworksNW, Seattle, WA.

P005HIGH TITER OF ANGIOTENSIN II RECEPTOR I ANTIBODIES MAY CAUSE PERSISTENT WEAK POSITIVE B CELL REACTIVITY IN FLOW CYTOMETRY CROSSMATCH IN THE ABSENCE OF HLA ANTIBODIESValia Bravo-Egana, Dimitri Monos, Children’s Hospital of Philadelphia, Philadelphia, PA.

P006ASSESSING THE FUNCTIONAL SIGNIFICANCE OF HLA ANTIBODIES IN RENAL ALLOGRAFTMichael Conciatori1, Michael Prod1, Maria Oppermann1, Maribeth Flaws1, Demetra C. Castillo2, Siva Kanangat1, 1Rush University Medical Center, Pathology, Chicago, IL, 2Rush University Medical Center, Medical Lab Sciences, Chicago, IL.

P007UTILITY OF AUTOLOGOUS CROSSMATCHES IN RESOLVING UNEXPECTED POSITIVE FLOW CYTOMETRY CROSSMATCHESDeanna Manna1, Jenny Elphick2, Anne Halpin1, Patricia Campbell1, Luis Hidalgo1, 1Alberta Health Services, Edmonton, AB, CANADA, 2University of Alberta, Edmonton, AB, CANADA.

P008CASE SCENARIO, PERFORMANCE DIFFERENCES OF THE TWO COMMERCIALLY AVAILABLE SOLID PHASE SINGLE ANTIGEN REAGENTSFadi Al Zayer, Amal Al-Gharably, Amro Adnan, Moheeb Al-Awwami, King Faisal Specialist Hospital and Research Center, Riyadh, SAUDI ARABIA.

P009IMPLEMENTATION OF ROBOTIC METHODOLOGY AND INSTRUMENTATION FOR THE DETECTION OF HLA ANTIBODIES IN SINGLE ANTIGEN BEADS ASSAYSCasiana Fernandez-Bango, Alina Gutierrez, Ana Hernandez, Emilio Margolles-Clark, Phillip Ruiz, University of Miami, Surgery. Transplant Laboratory, Miami, FL.


ABSTRACTSP010COMPLEX CONSTELLATION OF HLA ANTIBODIES CONTRIBUTES TO DECREASING TREND OF KIDNEY TRANSPLANT RATE IN CANDIDATES WITH 100% CPRARaja Rajalingam1, David D. Gae1, Dessislava Kopchaliiska1, Owen Buenaventura1, Kelly Cunniffe1, Gil Da Gente1, John Roberts2, 1Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA, 2Transplant Services, Department of Surgery, University of California San Francisco, San Francisco, CA.

P011THE HSA-MIR-150-3P MICRORNA IS UP-REGULATED IN GESTATIONAL DIABETES AND TARGETS THE HLA-G GENEEduardo A. Donadi1, Nathalia J. Cézar2, Jessica R. Plaça3, Juliana D. Massaro1, Luciana C. Veiga-Castelli1, Erick C. Castelli4, Claudia D. Polli1, Wilson A. Silva-Jr3, Elayne C. Moisés5, Geraldo Duarte5, Celso T. Mendes-Junior6, 1Faculty of Medicine of Ribeirão Preto - University of São Paulo, Department of Clinical Medicine, Ribeirao Preto, BRAZIL, 2Faculty of Medicine of Ribeirão Preto - University of São Paulo, Department of Genetics, Ribeirao Preto, BRAZIL, 3Faculty of Medicine of Ribeirão Preto - University of São Paulo, Regional Blood Center, Ribeirao Preto, BRAZIL, 4School of Medicine - UNESP São Paulo State University, Department of Pathology, Botucatu, BRAZIL, 5Faculty of Medicine of Ribeirão Preto - University of São Paulo, Department of Gynecology and Obstetrics, Ribeirao Preto, BRAZIL, 6Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Department of Chemistry, Ribeirao Preto, BRAZIL.

P012HUMAN IVIG PREPARATIONS CONTAIN ANTIBODY TO NATIVE AND DENATURED HLAMarilyn Marrari1, Massimo Mangiola1, Mel Berger2, Martin Spycher3, Adriana Zeevi1, 1University of Pittsburgh, Pittsburgh, PA, 2CSL Behring, LLC, King of Prussia, PA, 3CSL Behring AG, 3000 Bern 22, SWITZERLAND.

P013DEVELOPMENT OF THE IPD-MHC DATABASEJames Robinson1, Giuseppe Maccari2, Ronald E. Bontrop3, Chak-Sum (Sam) Ho4, Unni Grimholt5, Jim Kaufman6, Lisbeth A. Guethlein7, Keith Ballingall8, Steven G. Marsh1, John A. Hammond2, 1Anthony Nolan Research Institute & UCL Cancer Institute, London, UNITED KINGDOM, 2The Pirbright Institute, Guildford, UNITED KINGDOM, 3Biomedical Primate Research Centre, Rijswijk, NETHERLANDS, 4Gift of Life Michigan, Ann Arbor, MI, 5Norwegian Veterinary Institute, Oslo, NORWAY, 6Cambridge University, Cambridge, UNITED KINGDOM, 7Stanford University, Stanford, CA, 8The Moredun Research Institute, Edinburgh, UNITED KINGDOM.


ABSTRACTSP014A SIMPLE AND FAST METHOD FOR ENRICHMENT OF LYMPHOCYTE SUBSETS FOR COMPLEMENT DEPENDENT CYTOTOXICITY ASSAYSAdriana Salomon1, Phillip Rutsch1, Matthias Könn1, Annette von Borstel2, Vanessa Ditt2, Gregor Winkels1, 1Miltenyi Biotec GmbH, Bergisch Gladbach, GERMANY, 2Institute for Clinical Transfusion Medicine Merheim Medical Center, Cologne General Hospital, Transplant Center Cologne, Cologne, GERMANY.

P015IMPACT OF THE NEW KIDNEY ALLOCATION SYSTEM ON HIGHLY SENSITIZED RECIPIENT TRANSPLANT RATES: THE UTAH EXPERIENCEAnn Pole1, Julio C. Delgado2, Eszter Lazar-Molnar2, 1University of Utah Healthcare, Histocompatibility & Immunogenetics, Salt Lake City, UT, 2University of Utah, Pathology, Salt Lake City, UT.

P016OVERCOMING ELECTRONIC MEDICAL RECORD INTEROPERABILITY CHALLENGES WITH A COMBINED DISCRETE DATA AND ENCAPSULATED BINARY TEST RESULTS INTERFACECollin D. Brack1, Catherine Gebhart2, 1Belhaven University, Houston, TX, 2University of Nebraska, Pathology and Microbiology, Omaha, NE.

P017AUTOMATION OF HLA-TYPING USING NEXT GENERATION SEQUENCING WITH OMIXON’S HOLOTYPE HLA X2 KIT AND BECKMAN-COULTER’S BIOMEK LIQUID HANDLING SYSTEM.Robert Pollok, Omixon, Cambridge, MA.

P018EVALUATION OF LABTYPE CWD AND XR ASSAYS UTILIZING LABSCAN3D FOR HIGH THROUGHPUT/RESOLUTION HLA TYPINGKimberly Y. Levy1, Kathleen C. Barracchini2, Sharon D. Adams2, Willy A. Flegel2, 1National Institutes of Health, Bethesda, MD, 2National Institutes of Health, Department of Transfusion Medicine, Bethesda, MD.


ABSTRACTSP019COLLECTION OF MAJOR HLA ALLELE SEQUENCES IN JAPANESE POPULATION TOWARDS PRECISE NGS BASED HLA DNA TYPING AT THE FIELD 4 LEVEL Shingo Suzuki1, John Harting2, Primo Baybayan2, Ken Osaki3, Miwako Kitazume3, Junichi Sunaga3, Swati Ranade2, Takashi Shiina1, 1Tokai University School of Medicine, Molecular Life Science, Kanagawa, JAPAN, 2Pacific Biosciences, Molecular Biology Applications, Menlo Park, CA, 3Tomy Digital Biology, Pacific Biosciences Division, Tokyo, JAPAN.

P020EVALUATION OF 5-COLOR MULTIPLEX SHORT TANDEM REPEAT METHOD FOR CHIMERISM/ENGRAFTMENT STUDYLorie H. Kumer, Carolyn Fisher, Justine L. Gaspari, Ronald E. Domen, Hiroko Shike, Penn State Hershey Med Center, Pathology, Hershey, PA.

P021DEVELOPMENT OF LABORATORY-DEVELOPED CHIMERISM ANALYSIS ASSAY TARGETING T LYMPHOCYTES IN HEMATOPOIETIC STEM CELL TRANSPLANTATIONYun Ji Hong1,2, Mi Sun Lee2, Sun Young Kim3, Sang Mee Hwang1,2, Jeong Su Park1,2, Eun Young Song1, Kyoung Un Park1,2, Junghan Song1,2, 1Seoul National University College of Medicine, Laboratory Medicine, Seoul, KOREA, REPUBLIC OF, 2Seoul National University Bundang Hospital, Laboratory Medicine, Seongnam, KOREA, REPUBLIC OF, 3Chungnam National University College of Medicine, Laboratory Medicine, Daejeon, KOREA, REPUBLIC OF.

P022HLA-DRB1 AND HLA-DQA1/B1 ALLELES IN ASSOCIATION WITH LEPROSY COMPARED TO HOUSEHOLD CONTACTS.Hugo V. Alves1, Eliane P.A. P. Albuquerque2, Priscila S. Mazini2, Julimary S. Aquino2, André L. Lima2, Jeane E. Visentainer2, 1Universidade Estadual de Maringa, Ciencias Basicas da Saude, Maringá, BRAZIL, 2Universidade Estadual de Maringa, Ciencias Basicas da Saude, Maringa, BRAZIL.

P023DIRECT TRANSMISSION OF HLA REPORT TO ELECTRONIC MEDICAL RECORD VIA COLD FEED INTERFACEHiroko Shike, Justine Gaspari, Luann Newcomer, Ronald E. Domen, Tony Douventzidis, Penn State Hershey Med Center, Pathology, Hershey, PA.

P024AUTOMATED SOLID-PHASE HLA ANTIBODY ASSAY USING THE BIOTEK ELX50 MICROPLATE WASHER


ABSTRACTSKatarzyna Szewczyk, Yvonne M. Desmarteau, Andrés Jaramillo, Marcelo J. Pando, Mayo Clinic Hospital, Department of Laboratory Medicine and Pathology, Phoenix, AZ.

P025HLA-C AND HLA-P ANTIBODY IN RENAL TRANSPLANTED PATIENTS IN HONG KONGAlan Li, Karen Kwong, Jenny Ho, Janette Kwok, Queen Mary Hospital, Hong Kong, HONG KONG.

P026FOUR UNRELATED INDIVIDUALS SHARING A HAPLOTYPE WITH A REAR ALLELE DQB1*06:123Amal Al Gharably, Fadi Al Zayer, sahar Sandooqah, Maha Al-Harbi, Moheeb Al-Awwami, King Faisal Specialist Hospital and Research Center, Riyadh, SAUDI ARABIA.

P027AUTOMATION OF NGS-BASED HLA TYPING ASSAYS ON THE BECKMAN COULTER BIOMEK SERIES OF AUTOMATED LQUID HANDLERSZachary Smith, Shilpa Parakh, Beckman Coulter, Inc., Indianapolis, IN.

P028UNCOVERING THE INVISIBLE; PRE-TREATMENT OF SERA WITH EDTA RELIEVES PROZONE EFFECTWalter Herczyk, Mary Jackowski, Taba Kheradmand, Sam Ho, Gift of Life Michigan, Laboratory, Ann Arbor, MI.

P029ALGORITHM FOR HAPLOIDENTICAL HEMATOPOIETIC CELL TRANSPLANT IN PANAMAAlejandro A. Vernaza1, Ricardo Aguilar2, Ninoshka Mendoza2, Lineth Lopez2, Fernando Diaz2, Dimas Quiel1, Gabrielle Britton3, Alcibiades Villarreal4, German Espino2, Cesar Cuero5, Juan Moscoso1, Luis Ortiz1, Elena Blake1, Yina Gutierrez1, 1Caja de Seguro Social, Transplant Laboratory, Panama, PANAMA, 2Caja de Seguro Social, Hematology Service, Panama, PANAMA, 3INDICASAT, Panama, PANAMA, 4Caja de Seguro Social, INDICASAT, Panama, PANAMA, 5Caja de Seguro Social, Transplant Organization Panama, Panama, PANAMA.

P030HLA TOOL FOR TEACHINGLinda S. Buckert, Loma Linda University Medical Center, HLA, Loma Linda, CA.


ABSTRACTSP031LABORATORY ALGORITHM FOR THE FIRST CARDIAC TRANSPLANT IN PANAMA.Alejandro A. Vernaza1, Temistocles Diaz2, Manuel Ochoa3, Miguel Guerra3, Cesar Cuero4, Juan Moscoso1, Luis Ortiz1, Elena Blake1, Yina Gutierrez1, 1Caja de Seguro Social, Transplant Laboratory, Panama, PANAMA, 2Punta Pacifica Hospital Panama, Cardiologist, Panama, PANAMA, 3Caja de Seguro Social, Cardiologist, Panama, PANAMA, 4Caja de Seguro Social, Transplant Organization Panama, Panama, PANAMA.

P032DETERMINING THE MINIMUM EDTA CONCENTRATION NECESSARY TO ELIMINATE PROZONE EFFECT IN THE SINGLE ANTIGEN BEAD (SAB) ASSAY.Robert Liwski, Geoffrey Peladeau, Jennifer Peladeau, Kelly Heinstein, Dalhousie University, Halifax, NS, CANADA.

P033QUANTIFICATION OF CMV-SPECIFIC T CELL RESPONSES IN IMMUNOSUPPRESSED RENAL TRANSPLANT RECIPIENTSMaura Rossetti1, Yael Korin1, Tiffany Sidwell1, Gemalene Sunga1, Nicholas Harre1, Tara Sigdel2, Suphamai Bunnapradist3, Flavio Vincenti4, Minnie Sarwal2, Elaine Reed1, 1UCLA, Pathology and Laboratory Medicine, Los Angeles, CA, 2UCSF, Surgery, San Francisco, CA, 3UCLA, Medicine, Los Angeles, CA, 4UCSF, Medicine, San Francisco, CA.

P034DETECTION OF SOLUBLE HLA ANTIGENS (SHLA) IN BLOOD FOR EARLY DETECTION OF NON-SMALL CELL LUNG CARCINOMA (NSCLC)Smriti Kanangat1, Cristina Fhied2, Melissa Pergande1, Jeffrey Borgia1, Robert DeCresce3, Michele H. Prod3, 1Rush University Medical Center, Pathology/Biochemistry, Chicago, IL, 2Rush University Medical Center, Biochemistry, Chicago, IL, 3Rush University Medical Center, Pathology, Chicago, IL.

P035A METHOD FOR LARGE-SCALE ANALYSIS OF HLA GENETIC VARIATIONWei Wang1, Yung-Tsi Bolon1,2, Hu Huang1,2, Craig Malmberg1, Caleb Kennedy1,2, Martin Maiers1, 1National Marrow Donor Program, Minneapolis, MN, 2University of Minnesota, Minneapolis, MN.


ABSTRACTSP036WHOLE GENE SEQUENCING OF KIR-3DL1 WITH PACBIO RSII AND THE DISTRIBUTION OF ALLELEIC VARIANTS IN DIFFERENT ETHIC GROUPSNezih Cereb1, HwaRan KIM1, Jaejun Ryu1, Eunsil Kim1, Vikas Rai1, Seho Choi1, Cynthia Vierra-Green2, Stephen Spellman2, JeongOk Jeon1, Jangyoung Kwon1, Soo Young Yang1, 1Histogenetics, Ossining, NY, 2Center for International Blood and Marrow Transplant Research, Minneapolis, MN.

P037GENOTYPING OF SIX CLASSICAL HLA LOCI USING NEXT GENERATION SEQUENCINGPhilip Ehrenberg, Aviva Geretz, Ravi Kumar Sindhu, Rasmi Thomas, Walter Reed Army Institute of Research, US Military HIV Research Program, Silver Spring, MD.

P038“DONOR”-SPECIFIC REGULATORY T CELLS FOR CLINICAL TRANSPLANT TOLERANCE: OPTIMIZATION OF EX VIVO EXPANSION AND CHARACTERIZATIONJames M. Mathew1, Jessica L. Heinrichs1, Scott T. McEwen1,2, Iwona M. Konieczna1, Xuemei Huang1, Jie He1, Richard S. Kornbluth3, Joshua Miller1, Joseph R. Leventhal1, 1Northwestern University, Surgery - Comprehensive Transplant Center, Chicago, IL, 2Case Western Reserve University, Pediatrics, Cleveland, OH, 3Multimeric Biotherapeutics, Inc, La Jolla, CA.

P039NEW PRIORITIES: ANALYSIS OF THE NEW KIDNEY ALLOCATION SYSTEM ON UCLA PATIENTS TRANSPLANTED FROM THE DECEASED DONOR WAITLISTMichelle J. Hickey1, Ying Zheng1, Carolyn Krystal2, Nicole Valenzuela1, Eileen Tsai3, Qiuheng Zhang1, Jeffrey Veale4, David Gjertson1, Michael Cecka1, Elaine F. Reed1, 1UCLA Immunogenetics Center, Pathology and Laboratory Medicine, Los Angeles, CA, 2UCLA, Integrative Biology and Physiology, Los Angeles, CA, 3UCLA, Pediatrics-Nephrology, Los Angeles, CA, 4UCLA David Geffen School of Medicine, Urology, Los Angeles, CA.

P040BINDING OF CARBAMYLATED PEPTIDES TO ALLELES ASSOCIATED WITH SUSCEPTIBILITY AND RESISTANCE TO RHEUMATOID ARTHRITISKirsten M. Anderson1, Tiana Stastny2, Christina Roark1, Michael Aubrey1, Brian Freed1, 1University of Colorado Denver, Immunology, Aurora, CO, 2University of Colorado Denver, Aurora, CO.


ABSTRACTSP041NEXT GENERATION SEQUENCING REVEALS HLA GENOMIC AND HAPLOTYPE DIVERSITY IN U.S. POPULATIONS OF EUROPEAN AND AFRICAN ANCESTRY.Lisa E. Creary1, Sujatha Krishnakumar2, Chunlin Wang2, Ming Li2, Alicia Sanchez-Mazas3, Jose M. Nunes3, Douglas F. Levinson4, Michael N. Mindrinos2, Marcelo A. Fernandez-Vina1, 1Stanford University School of Medicine, Pathology, Palo Alto, CA, 2Sirona Genomics Inc, Mountain View, CA, 3Anthropology Unit and Institute of Genetics and Genomics in Geneva, Genetics and Evolution, Geneva, SWITZERLAND, 4Stanford University School of Medicine, Psychiatry, Palo Alto, CA.

P042IMPACT OF MINOR HLA ALLELIC MISMATCHES ON OUTCOMES OF HEMATOPOIETIC CELL TRANSPLANTSAndrew L. Lobashevsky, Sherif S. Farag, Rafat Abonour, Robert P. Nelson, Paul R. Haut, Kent A. Robertson, Jennifer E. Schwartz, David C. Delgado, Jodi L. Skiles, Michael J. Robertson, Scott W. Goebel, IU, Medicine, Indianapolis, IN.

P043HLA-DP DONOR SPECIFIC ANTIBODIES ASSOCIATED WITH ANTIBODY MEDIATED REJECTION AND POOR ALLOGRAFT SURVIVAL FOLLOWING LUNG TRANSPLANTAiwen Zhang1, Paul Kawczak1, Yuchu Sun2, Dawn Thomas1, Nathan Stopczynski1, John McMichael1, Kenneth McCurry3, Maria Budev4, 1Cleveland Clinic, Allogen Laboratories, Cleveland, OH, 2Avalon Medical University, Youngstown, OH, 3Cleveland Clinic, Thoracic and Cardiovascular Surgery, Cleveland, OH, 4Cleveland Clinic, Pulmonary, Allergy and Critical Care Medicine, Cleveland, OH.

P044C1Q STATUS AND TITER OF DE NOVO DONOR SPECIFIC ANTIBODIES ARE NOT PREDICTORS OF ALLOGRAFT SURVIVALChris Wiebe1,2, Alison Gareau1, Denise Pochinco2, Ian Gibson1,2, Julie Ho1, Patricia Birk1, Tom Blydt-Hansen3, Martin Karpinski1, Aviva Goldberg1, Leroy Storsley1, David Rush1, Peter Nickerson1,2, 1University of Manitoba, Winnipeg, MB, CANADA, 2Diagnostic Services of Manitoba, Winnipeg, MB, CANADA, 3University of British Columbia, Vancouver, BC, CANADA.

P045VALIDATION OF BLDTYPE FOR ABO/RHD/CCR5 TYPING OF REGISTRY DONORSWei Dong1, Sam Ho2, Christina Tan3, Elise MacLeod2, Robert Bresler2, Margaret Keller3, Susan Hsu1, 1American Red Cross Biomedical Services, Penn-Jersey Region,


ABSTRACTSHistocompatibility/Molecular Genetics, Philadelphia, PA, 2Mid-America Transplant, St. Louis, MO, 3American Red Cross Biomedical Services, Penn-Jersey Region, National Molecular Laboratory, Philadelphia, PA.

P046DECONSTRUCTING HLA-C MISMATCH IN HEMATOPOIETIC STEM CELL TRANSPLANTATIONChrysanthi Tsamadou1,2, Daniel Fürst1,2, Dietger Niederwieser3, Donald Bunjes4, Christine Neuchel1,2, Martin Gramatzki5, Renate Arnold6, Eva Wagner7, Hermann Einsele8, Hubert Schrezenmeier1,2, Joannis Mytilineos1,2,9, 1Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, Transplantation Immunology, Ulm, GERMANY, 2Institute of Transfusion Medicine, University of Ulm, Ulm, GERMANY, 3University of Leipzig, Department of Hematology/Oncology, Leipzig, GERMANY, 4University of Ulm, Department of Internal Medicine III, Ulm, GERMANY, 5University of Kiel, Division of Stem Cell Transplantation and Immunotherapy, 2nd Department of Medicine, Kiel, GERMANY, 6Charité Campus Virchow Berlin, Hematology/Oncology Department, Berlin, GERMANY, 7Johannes Gutenberg-University Mainz, Department of Internal Medicine III, Mainz, GERMANY, 8University Hospital Würzburg, Department of Internal Medicine II, Würzburg, GERMANY, 9DRST – German Registry for Stem Cell Transplantation, Ulm, GERMANY.

P047ANALYSIS OF 25,000 CAUCASIAN SAMPLES REVEALS TIGHT LINKAGE BETWEEN SNP RS9277534 AND HIGH RESOLUTION TYPING OF HLA-DPB1Bianca Schöne1, Kathrin Lang1, Sabine Bergmann1, Lisa Hedrich1, Johanna M. Andreas1, Alexander H. Schmidt1,2, Vinzenz Lange1, Effie W. Petersdorf3, 1DKMS Life Science Lab GmbH, Dresden, GERMANY, 2DKMS, Tübingen, GERMANY, 3Fred Hutchinson Cancer Research Center, Seattle, WA.

P048GENE EXPRESSION PROFILING OF TRANSPLANT FORMALIN-FIXED PARAFFIN-EMBEDDED BIOPSIES: COMPARISON OF A CUSTOM TAQMAN® LOW DENSITY ARRAY AND QUANTITATIVE NUCLEASE-PROTECTION ASSAYEmilio Margolles-Clark Margolles-Clark, Phillip Ruiz, University of Miami, Surgery. Transplant Laboratory, Miami, FL.

P049HUMAN METABOLITES BIND TO THE P4 POCKET OF HLA-DRB1*15:01, WITH IMPLICATIONS FOR MULTIPLE SCLEROSIS SUSCEPTIBILITYManeesh K. Misra, Jill A. Hollenbach, University of California San Francisco, Department of Neurology, San Francisco, CA.


ABSTRACTSP050DSA-FXM DISTINGUISH AUTO-AB FROM ALLO-AB IN FXM AND DETECT TRUE DONOR SPECIFIC HLA-ABSGe Chen, Lingling Lin, Dolly Tyan, Stanford, Palo Alto, CA.

P051THE EVOLUTIONARY AGE OF ANTI-HLA ANTIBODY EPITOPESPedro Cano, Moffit Cancer Center, Tampa, FL.

P052IN SEARCH OF A GOLD STANDARD FOR A LOW IMMUNE RISK KIDNEY TRANSPLANT. FLOW CYTOMETRY CROSSMATCH OR SINGLE ANTIGEN BEADS?Juliana Montagner1, Aline Gasparotto1, Elizete Keitel2, Valter Garcia2, Jorge Neumann1, 1Santa Casa Hospital, Lab of Transplantation Immunology, Porto Alegre, BRAZIL, 2Santa Casa Hospital, Renal Transplant Unit, Porto Alegre, BRAZIL.

P053PRE-TREATMENT DSA TITER PREDICTS THE EFFECTIVENESS OF CARFILZOMIB-BASED THERAPY FOR ANTIBODY MEDIATED REJECTION IN LUNG TRANSPLANT RECIPIENTSAdriana Zeevi, Marilyn Marrari, Massimo Mangiola, Matthew R. Morrell, Samuel A. Yousem, Joseph M. Pilewski, Jonathan D’Cunha, John F. McDyer, Christopher R. Ensor, University of Pittsburgh, Pittsburgh, PA.

P054EDTA TREATMENT OF SERUM ELIMINATES INTERFERENCE IN FLOW CYTOMETRIC CROSSMATCHESMichael J. Rewinski, Julio Ortiz, Tod Alberghini, Laurine Bow, Joe Devivo, Pam Doyle, Trish Michalski, Hartford Hospital, Transplant Immunology, Hartford, CT.

P055DEVELOPMENT OF IMMUNE RESPONSES TO TISSUE-RESTRICTED SELF-ANTIGENS IN SIMULTANEOUS KIDNEY-PANCREAS TRANSPLANT RECIPIENTS WITH ACUTE REJECTIONMuthukumar Gunasekaran1, Neeta Vachharajani2, Joseph Gaut3, Donna Phelan4, Thin Thin Maw5, Rowena Delos Santos5, Surendra Shenoy6, William Chapman6, Jason Wellen6, Thalachallour Mohanakumar1, 1St. Joseph’s Hospital & Medical Center, Norton Thoracic Institute, Phoenix, AZ, 2Washington University School of Medicine, Surgery, St Louis, MO, 3Washington University School of Medicine, Pathology, St. Louis, MO, 4Barnes-Jewish Hospital, HLA Laboratory, St. Louis, MO, 5Washington University School of Medicine, Medicine, St. Louis, MO, 6Washington University School of Medicine, Surgery,


ABSTRACTSSt. Louis, MO.

P056SIMULTANEOUS HLA CLASS I AND II SEQUENCING BY LONG READ-INDEPENDENT CIRCULAR CONSENSUS SMRT TECHNOLOGYChee Loong Saw1, Haig Djambazian2, Ioannis Ragoussis2, 1McGill University Health Centre, HLA Lab, Montreal, QC, CANADA, 2McGill University Genome Quebec and Innovation Centre, Montreal, QC, CANADA.

P057MEASURING LINKAGE DISEQUILIBRIUM WITH NEXT-GENERATION SEQUENCING HLA TYPING DATAPedro Cano, Moffit Cancer Center, Tampa, FL.

P058VALIDATION OF ANTI-HUMAN ANTIBODIES USED IN FLOW CYTOMETRIC CROSSMATCHINGPaul Warner1, Linda Kapp2, Heather Howie2, James Zimring2, 1Bloodworks Northwest, Immunogenetics/HLA, Seattle, WA, 2Bloodworks Northwest, Research Institute, Seattle, WA.

P059ANALYSIS OF THE ONE YEAR TRANSPLANT TREND IN MICHIGAN’S HIGHLY SENSITIZED PATIENT COHORT POST NEW OPTN KIDNEY ALLOCATION SYSTEMTaba Kheradmand, Sam Ho, Gift of Life Michigan, Ann Arbor, MI.

P060ANTIBODY-MEDIATED AND CELLULAR REJECTION: HOW CAN THEY SEEM SO SIMILAR?Michael Parkes1, Phil F. Halloran1, Luis G. Hidalgo2, 1University of Alberta, Medicine, Edmonton, AB, CANADA, 2University of Alberta, Lab. Med. and Pathology, Edmonton, AB, CANADA.

P061DONOR-SPECIFIC ANTI-HUMAN LEUKOCYTE ANTIBODIES OF IMMUNOGLOBULIN G3 SUBTYPE ARE ASSOCIATED WITH HIGH-GRADE REJECTION IN PEDIATRIC HEART TRANSPLANTATIONAlin Girnita1, Elizabeth Portwood2, Paul Brailey3, Sairah Khan4, Andrei Tica5, Clifford Chin6, 1University of Cincinnati COM, Transplant Immunology Division, Cincinnati, OH, 2Hoxworth Blood Center, Transplant Immunology Division, Cincinnati, OH,


ABSTRACTS3Hoxwworth Blood Center, Transplant Immunology Division, Cincinnati, OH, 4Childrens Hospital, Cardiology, Cincinnati, OH, 5University of Medicine and Pharmacy Craiova, Pharmacology, Craiova, ROMANIA, 6Cincinnati Children’s Hospital Medical Center, The Heart Institute, Cincinnati, OH.

P062EVALUATION OF HLA-DP T-CELL EPITOPE (TCE) MATCHING ALGORITHM FOR SELECTING DONORS BMT/HSCTAnish Laul, Gopal Patel, Ina Kurbegovic -Skaljic, Sylvia Piggott, Siva Kanangat, Rush University Medical Center, Pathology, Chicago, IL.

P063WHAT’S NEW ON ALLELEFREQUENCIES.NET (AFND)Derek Middleton1, Steven J. Mack2, 1Royal Liverpool Hospital, H&I, Liverpool, UNITED KINGDOM, 2Children’s Hospital Oakland Research Institute, Oakland, CA.

P064SERUM TREATMENT IN A FLOW CROSSMATCHDonna Lucas, Annette Jackson, Mary Carmelle Philogene, Johns Hopkins University School of Medicine, Immunogenetics, Baltimore, MD.

P065INFORMATION THEORY-BASED ANALYSIS OF CLASSICAL HLA GENESHu Huang1,2, Wei Wang2, Yung-Tsi Bolon2,1, Craig Malmberg2, Caleb Kennedy2,1, Martin Maiers2, 1University of Minnesota, Bioinformatics and Computational Biology, Minneapolis, MN, 2National Marrow Donor Program, Bioinformatics Research, Minneapolis, MN.

P066NEXT-GENERATION SEQUENCING (NGS) HLA TYPING: BEYOND ALLELE ASSIGNMENTPedro Cano1, Ming Li2, 1Moffit Cancer Center, Tampa, FL, 2IMMUCOR, Mountain View, CA.

P067HOW DIFFERENT IS GAMMA BLOCK IN HLA MATCHED (10/10) UNRELATED PAIRS DETECTED BY NEXT GENERATION SEQUENCING (NGS)?Ketevan Gendzekhadze1, Hayley Hogan2, Carla Wirtz2, Jean Garcia-Gomez1, David Sayer2, David Senitzer1, 1City of Hope, HLA laboratory, Duarte, CA, 2Illumina, Inc, San Diego, CA.


ABSTRACTSP068TRANSPLANT TOLERANCE IN HEART TRANSPLANT RECIPIENTS: IT IS ALL IN THE GENESPrakash Rao1, David Baran2, Dayanand Deo1, Khurram Liaquat2, Janice Pieretti2, Claudia Gidea2, Mrudula Munagala2, Mark Zucker2, 1NJ Sharing Network, Transplant Laboratory, New Providence, NJ, 2Newark Beth Israel Medical Center, Newark, NJ.

P069NEXT GENERATION SEQUENCING BASED POLYMORPHISM AND GENE EXPRESSION ANALYSIS OF UMBILICAL CORD BLOOD HUMAN LEUKOCYTE ANTIGENFiona F. Yamamoto1,2, Dolly B. Tyan3,4, Marcelo Fernandez-Viña1,5, Shingo Suzuki6, Takashi Shiina7, Shunichi Kato8, Minoru Kimura7, 1Stanford Healthe Care, HIDPL, Stanford Blood Center, Palo ALto, CA, 2Tokai University School of Medicine, Department of Molecular Life Science, Kanagawa, JAPAN, 3Stanford University, Pathology, Palo ALto, CA, 4Stanford Health Carre, HIDPL, Stanford Blood Center, Palo Alto, CA, 5Stanford University, Department of Pathology, Palo Alto, CA, 6Tokai University School of Medicine, Department of Molecular Life Science, Kanagawa, JAPAN, 7Tokai University School of Medicine, Department of Molecular Life Science, Isehara, JAPAN, 8Tokai University School of Medicine, Department of Cell Transplantation and Regenerative Medicine, Isehara, JAPAN.

P070EXTRACTING HISTOTRAC DATA TO EXCEL REDUCES THE TIME REQUIRED TO PERFORM VIRTUAL MATCHES BY SEMI-AUTOMATIONGizem Tumer, Randall Thorson, David H. Maurer, Lael Gatewood, Michael Lougee, Claudia S. Cohn, Univeristy of Minnesota, Minneapolis, MN.

P071CONTAMINATING DNA IS VARIABLY DETECTED AT EACH HLA GENE LOCUS BY NEXGEN SEQUENCING AND CAN RESULT IN INCORRECT HLA TYPE ASSIGNMENTSBobbie Rhodes-Clark, Soumya Pandey, Terry Harville, University of Arkansas for Medical Sciences, Little Rock, AR.

P072DE NOVO ANTI-HLA-CLASS II DONOR SPECIFIC ANTIBODIES ARE ASSOCIATED WITH PATIENT MORTALITY AFTER LUNG TRANSPLANT FAILUREBurak Bahar1, Daniel F. Dilling2, Zeying Du1, 1Loyola University Medical Center, Pathology, Maywood, IL, 2Loyola University Medical Center, Pulmonary and Critical Care Medicine, Maywood, IL.


ABSTRACTSP073NEXT GENERATION SEQUENCING OF ALL CLASSICAL HLA-CLASS I AND II GENESNathaniel T. Smith1, Vinh Ngo1, Yudith Carmazzi1, Sujatha Krishnakumar2, Ming Li2, Chunlin Wang2, Edward Guerrero1, Michael Mindrinos2, Kai Cao1, 1UT MD Anderson Cancer Center, Laboratory Medicine - HLA Typing Laboratory, Houston, TX, 2Immucor Sirona Genomics, Mountain View, CA.

P074DONOR SPECIFIC ANTI-HLA ANTIBODIES ARE ASSOCIATED WITH SUBCLINICAL ALLOGRAFT INJURY IN HEART TRANSPLANT RECIPIENTSHugo Kaneku Nagahama, Cesar Y. Guerrero-Miranda, Peter Masiakos, James Pullman, Ulrich Jorde, Adriana Colovai, Montefiore Medical Center, Bronx, NY.

P075GENERATION OF AN ALGORITHM TO STANDARDIZE HLA TYPING INFORMATION FOR UNOSNicholas K. Brown, Rebecca L. Upchurch, Jerome G. Weidner, Susana R. Marino, University of Chicago, Pathology, Chicago, IL.

P076KIR ALLELE LEVEL TYPING BY A NOVEL NEXTYPE ALGORITHMJens Pruschke1, Ines Wagner2, Christine Gnahm1, Daniel Schefzyk1, Bianca Schöne2, Kathrin Lang2, Paul J. Norman3, Jill A. Hollenbach4, Jan A. Hofmann1, Jürgen Sauter1, Vinzenz Lange2, Julia Pingel1, Alexander H. Schmidt1, 1DKMS, Tübingen, GERMANY, 2DKMS Life Science Lab, Dresden, GERMANY, 3Stanford University School of Medicine, Stanford, CA, 4University of California, San Francisco School of Medicine, Department of Neurology, San Francisco, CA.

P077ANTI-A TITERS IN ABO INCOMPATIBLE TRANSPLANTATION - AN OPO’S EXPERIENCEMaria Aguilucho1, Donna King1, Misty Marchioni1, Bridget Figueiredo1, Ijeoma Okere1, Adena J. Osband2, Prakash Rao1, 1NJ Sharing Network, New Providence, NJ, 2Robert Wood Johnson University Hospital, New Brunswick, NJ.


ABSTRACTSP078THE RELATIONSHIP BETWEEN MIHAS COMPATIBILITY AND HLA MATCHING DEGREE OF RELATED DONOR AND RECIPIENT PAIRS IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATIONYuying Sun, Hongyan Wang, Yongzhi Xi, Beijing 307 Hospital, Beijing, CHINA.

P079EXPERIENCE USING THE ILLUMINA HLA TRUSIGHT NGS KITS: VERSION 1 VS. VERSION 2Tracie Profaizer, Eszter Lazar-Molnar, Julio C. Delgado, Attila Kumánovics, ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT.

P080HLA DISEASE ASSOCIATION ASSIGNMENT BY MULTIPLEXED NGS ASSAYNienke Westerink, Inge van Rooy, Michelle Van Heck, Claudia Rebel, Erik H. Rozemuller, Maarten T. Penning, GenDx, R&D, Utrecht, NETHERLANDS.

P081THE FEATURE SERVICE: A COMMUNITY RESOURCE FOR AUTOMATED ANNOTATION OF HLA & KIR SEQUENCE VARIANTSMartin Maiers1, Erik Pearson1, Pradeep Bashyal1, Caleb Kennedy1, Michael Heuer2, Robert P. Milius1, Steven J. Mack3, 1National Marrow Donor Program, Bioinformatics Research, Minneapolis, MN, 2University of California, AMP Lab, Berkeley, CA, 3Children’s Hospital Oakland Research Institute, Center for Genetics, Oakland, CA.

P082THE ROBUST NGSGO WORKFLOW FOR HIGH-RESOLUTION HLA TYPINGS ALSO FULLY COMPATIBLE WITH THE ION S5 PLATFORM Evelien Bouwmans1, Tina Hartvig2, Thomas B. Rasmussen2, Erik H. Rozemuller1, Hans O. Madsen2, Nienke Westerink1, Maarten T. Penning1, 1GenDx, R&D, Utrecht, NETHERLANDS, 2Rigshospitalet, Department of Clinical Immunology, Copenhagen, DENMARK.


ABSTRACTSP083TOOLS OF THE TRADE: HOW ANTIGEN DENSITY STACKS UP ON SINGLE ANTIGEN BEADS, A COMPARISON OF TWO VENDORSAnne Halpin, Luis Hidalgo, Christine Yamniuk, Deanna Manna, Patricia Campbell, University of Alberta Hospital, Histocompatibility, Edmonton, AB, CANADA.

P084POST TRANSPLANT DSA IN A COHORT OF KIDNEY RECIPIENTS 8 TO 16 YEARS AFTER TRANSPLANTATIONAllen J. Norin1, Laura Gutierrez Quiceno2, David Hochman3, Fasika Tedla4, Devon John5, Nabil Sumrani5, Moro Salifu4, Ballabh Das3, 1SUNY Downstate Medical Center, Brooklyn, NY, 2SUNY Downstate Medical Center, Trasnplant Laboratory, Brooklyn, NY, 3SUNY Downstate Medical Center, Pathology, Transplant Laboratory, Brooklyn, NY, 4SUNY Downstate Medical Center, Medicine, Brooklyn, NY, 5SUNY Downstate Medical Center, Surgery, Brooklyn, NY.

P085HEAT MAP OF HLA ANTIBODY PROFILES IN 5281 KIDNEY WAIT LIST CANDIDATES REVEALED LOCUS/CREG/BEAD-SPECIFIC PATTERNS ASSOCIATED WITH MODE OF SENSITIZATION.David D. Gae1, Dessislava Kopchaliiska1, Haibo Sun1, Owen Buenaventura1, Kelly Cunniffe1, Gil Da Gente1, John Roberts2, Raja Rajalingam1, 1Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA, 2Transplant Services, Department of Surgery, University of California San Francisco, San Francisco, CA.

P086DISTRIBUTION ANALYSIS OF DPB1*104:01 - UNEXPECTED RESULTS FOR A MEMBER OF THE DPB1*03:01:01G AMBIGUOUS ALLELE GROUPZachary Antovich1, Komal Singh1, Roland Russnak1, Wayne Shumway2, Sam Ho3, Renata Santos4, Angelica DeOliviveira4, 1Linkage Biosciences, Research and Development, South San Francisco, CA, 2LifeLink Foundation, Tampa, FL, 3Gift of Life Michigan, Ann Arbor, MI, 4Duke University Health Systems, Durham, NC.

P087HLA-DPB1 ALLELE TYPING FREQUENCY FROM A SINGLE CENTER STUDYPaul Kawczak1, Dawn Thomas1, Heather Eilrich1, Raymond Jurcago1, David Plunkett1, John McMichael1, Yuchu Sun2, Aiwen Zhang1, 1Cleveland Clinic, Allogen Laboratories, Cleveland, OH, 2Avalon Medical University, Youngstown, OH.


ABSTRACTSP088MULTIDIMENSIONAL IDENTIFICATION OF ANTI-HLA ANTIBODIESPedro Cano1, Stephanie Souza2, Ian Scott3, 1Moffit Cancer Center, Tampa, FL, 2Phoenix Childrens Hospital, Phoenix, AZ, 3Moffit Cancer Center, Tempe, AZ.

P089ENHANCED RESOLUTION IN RAPID HLA TYPING OF DECEASED DONORS: A SINGLE CENTER EXPERIENCE USING A NEW TAQMAN-BASED QPCR APPROACHJohn Lunz1, Marilyn Wetmore2, Ben Passey3, David Freedom1, Gordon Hill2, 1Gift of Hope Organ & Tissue Donor Network, Itasca, IL, 2Olerup, Inc, West Chester, PA, 3Olerup SSP AB, Stockholm, SWEDEN.

P090NGS CHARACTERIZATION OF HLA HAPLOTYPES IN MULTI-GENERATION FAMILIES OF KUWAITI DESCENTReem Ameen1, Salem Al-Shemmari2, Shawna Kennedy3, Medhat Askar3, 1Kuwait University, Medical Laboratory Sciences Department, Health Sciences Center, Jabriya, KUWAIT, 2Kuwait University, Department of Medicine, Health Sciences Center, Jabriya, KUWAIT, 3Baylor University Medical Center, Transplant Immunology, Pathology, Dallas, TX.

P091COMPARISON OF FOUR HLA NEXT-GENERATION SEQUENCING TYPING METHODSTracie Profaizer, Eszter Lazar-Molnar, Julio C. Delgado, Attila Kumánovics, ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT.

P092APOL1 GENOTYPING: CAN IT AID IN DONOR SELECTION?Elaine F. O’Shields1, Nicholette D. Palmer2, Barry I. Freeman3, David F. Kiger1, Michael D. Gautreaux1, 1Wake Forest School of Medicine, Pathology and Laboratory Medicine, Winston-Salem, NC, 2Wake Forest School of Medicine, Biohemistry, Winston-Salem, NC, 3Wake Forest School of Medicine, Internal Medicine, Winston-Salem, NC.

P093INCREASING NUMBER OF HIGHLY SENSITIZED PATIENTS WAITING IN KIDNEY PAIRED DONATION PROGRAMStanislaw Stepkowski1, Beata Mierzejewska2, Dulat Bekbolsynov1, Michael Rees1, 1University of Toledo, Medical Microbiology and Immunology, Toledo, OH, 2University of Toledo, Urology, Toledo, OH.


ABSTRACTSP094HUMAN LEUKOCYTE ANTIGEN CHARACTERIZATION OF A KUMEYAAY ETHNIC GROUP COMMUNITY IN BAJA CALIFORNIA, MEXICOBrenda L. Alvarado1, Karol Carrillo2, Horacio Almanza1, Hilario Flores3, Carmen Alaez4, 1Autonomous University of Baja California, Medicine and Psychology, Tijuana, MEXICO, 2National Institute of Genomic Medicine, Traductional Medicine, Mexico, MEXICO, 3Diagnostic and Epidemiologic Reference National Institute, Immunology, México, D.F., MEXICO, 4National Institute of Genomic Medicine, Traductional Medicine, México, D.F., MEXICO.

P095HLA CLASS I VARIATION IN IRANIAN LUR AND KURD POPULATIONS: HIGH ALLELE AND HAPLOTYPE DIVERSITY WITH AN ABUNDANCE OF KIR LIGANDSElham Ashouri1,2, Paul J Norman1, Lisbeth A Guethlein1, Alex Seungmo Han1, Neda Nemat-Gorgani1, Abbas Ghaderi3, Peter Parham1, 1Stanford University School of Medicine, Structural Biology, Stanford, CA, 2Shiraz University of Medical Sciences, Endocrinology and Metabolism Research Center, Shiraz, IRAN, ISLAMIC REPUBLIC OF, 3Shiraz University of Medical Sciences, Shiraz Institute for Cancer Research, Shiraz, IRAN, ISLAMIC REPUBLIC OF.

P096HLA-DPB1 LEVEL OF EXPRESSION, DETERMINED BY SNP ANALYSIS, AND SEROLOGIC EPITOPESPedro Cano, Moffit Cancer Center, Tampa, FL.

P097HIGH PREVALENCE OF ANTI-ANGIOTENSIN II TYPE 1 RECEPTOR ANTIBODY IN HLA-SENSITIZED TRANSPLANT CANDIDATESAlin Girnita1, Elizabeth Portwood1, Paul Brailey1, Rita Alloway2, ES Woodle3, 1University of Cincinnati COM, Transplant Immunology Division, Cincinnati, OH, 2University of Cincinnati COM, Internal Medicine, Cincinnati, OH, 3University of Cincinnati COM, Transplant Surgery, Cincinnati, OH.

P098HLA HAPLOTYPE DIVERSITY IN CAPE TOWN, SOUTH AFRICAYvonne Thorstenson1, Lisa Creary2, Huang Huang3, Virginie Rozot4, Chunlin Wang1, Ming Li1, Sandeep Kancharla5, Marilyn Fukushima1, Raquel Kuehn1, Sujatha Krishnakumar1, Michael Mindrinos1, Thomas J. Scriba4, Mark M. Davis6, 1Immucor, Sirona Genomics, Mountain View, CA, 2Stanford University, Histocompatibility Immunogenetics and Disease Profiling Laboratory, Palo Alto, CA, 3Stanford University, Stanford, CA, 4South


ABSTRACTSAfrican Tuberculosis Vaccine Initiative, Cape Town, SOUTH AFRICA, 5Sirona Genomics, Mountain View, CA, 6Stanford University, Howard Hughes Medical Institute, Stanford, CA.

P099THE IMPACT OF LYMPHOCYTE PURITY ON FLOW CYTOMETRY CROSSMATCH (FCXM) ASSAY. IT’S NOT PURELY THEORETICAL.Robert Liwski, Geoffrey Adams, Geoffrey Peladeau, Kelly Heinstein, Dalhousie University, Halifax, NS, CANADA.

P100RESISTANCE TO BK VIREMIA IN RENAL TRANSPLANT PATIENTS IS ASSOCIATED WITH HLA-DQ5 AND HLA-DQ6.Christina Roark1, Pratik Shah2, Alexander Wiseman2, Kirsten Anderson2, Michael Aubrey1, Brian Freed1, 1ClinImmune Labs, Aurora, CO, 2University of Colorado Anschutz Medical Campus, Aurora, CO.

P101HOW TO DO MORE WITH LESS IN THE MOLECULAR HLA LABORATORY?Jennifer McCue, Cassiana Fernandez-Bango, Raquel Cabanas, Eduardo Pozas, Maureen Harte, Ana Hernandez, Phillip Ruiz, University of Miami, Surgery. Transplant Laboratory, Miami, FL.

P102PREVALENCE OF ANTI HLA ANTIBODIES IN ADULT HEMATOPOIETIC STEM CELL TRANSPLANT RECIPIENTSMoheeb Al-Awwami1, Hana Al Khabbaz2, Mahmoud Aljurf3, Fadi Alzayer1, Feras Alfraih3, Walid Rasheed3, Naeem Chaudhri3, Fahad Almohareb3, Hazzaa Alzahrani3, Ghuzayel Aldawsari3, 1King Faisal Specialist Hospital and Research Center, Histocompatibility and Immunogenetics, Riyadh, SAUDI ARABIA, 2Riyadh Colleges for Dentistry and Pharmacy, Pharmacy and Allied Medical Sciences, Riyadh, SAUDI ARABIA, 3King Faisal Specialist Hospital and Research Center, Adult Hematology/Oncology and Stem Cell Transplantation, Riyadh, SAUDI ARABIA.

P103CROSSLINKING IMMUNOGENETICS TO PHARMACOGENETICS: FCΓRIIIA POLYMORPHISM PREDICTS RESPONSE TO RITUXIMAB THERAPY IN MANTLE CELL LYMPHOMA Ariz Akhter1, Taylor J. Kemp1, Gaurav Tripathi1, Rehan M. Faridi1, Douglas A. Stewart2, Adnan Mansoor1, Faisal M. Khan1, 1University of Calgary, Pathology and Laboratory


ABSTRACTSMedicine, Calgary, AB, CANADA, 2University of Calgary, Oncology and Medicine, Calgary, AB, CANADA.

P104OPTIMIZING AN NGS ASSAY FOR HLA TYPING TO ENSURE EVEN AMPLICON AND SAMPLE REPRESENTATIONNicholas K. Brown, Bradley C. Long, Brenda M. Issangya, Tenisha A. West, Rebecca L. Upchurch, Jerome G. Weidner, Jeremy P. Segal, Susana R. Marino, University of Chicago, Pathology, Chicago, IL.

P105LABORATORY STAFFING RETENTION: A RETROSPECTIVE STUDYKevin Chesterton, Karl Schillinger, Maria Bettinotti, Annette Jackson, Brian Iglehart, Johns Hopkins University, Baltimore, MD.

P106KILLER IMMUNOGLOBILUN-LIKE RECEPTORS (KIR) BB HAPLOTYPE CONFERS SUSCEPTIBILITY TO ACUTE CHILDHOOD LYMPHOBLASTIC LEUKEMIA (ALL) AND 2DS2, 2DS3 AND 2DS4 ACTIVATING GENES ARE OF LOW RISK IN MEXICAN CHILDRENBetsy A. Gonzalez1,2, Felicitas Manzanares1, Aida Delgado2, Andrea Munguia1, Hilario Flores-A1,2, Jaime Dulon1, David Senitzer3, Clara Gorodezky1,2, 1Instituto de Diagnóstico y Referencia Epidemiológicos, Mexico City, Mexico, MEXICO, 2Fundación Comparte Vida A.C., Mexico City, MEXICO, 3City of Hope National Cancer Center, Histocompatibility Laboratory, Duarte, CA.

P107NEXT GENERATION SEQUENCING (NGS) OF THE MHC, IN MESTIZOS FROM OAXACA, BELONGING TO THE MEXICAN UNRELATED DONOR REGISTRY-DONORMOAndrea Munguía1, Lisa E. Creary2, Hilario Flores-Aguilar1,3, Betsy A. Gonzalez1,3, Sridevi Gangavarapu4, Michael Mindrino5, Marcelo Fernandez-Vina4, Clara Gorodezky1,3, 1Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health., Department of Immunology and Immunogenetics, CDMX, México, MEXICO, 2Stanford University, School of Medicine, Department of Pthology, Palo Alto, CA, 3Fundación Comparte Vida, A.C., CDMX, México, MEXICO, 4Stanford University, School of Medicine, Department of Pathology, Palo Alto, CA, 5Sirona Genomics, Inc., Mountain View, CA.


ABSTRACTSP108PLATFORM-INDEPENDENT SOFTWARE AND REAGENTS FOR QPCR-BASED MICROCHIMERISM ANALYSISMark Reardon, Doug Bost, Jeta Molecular, Utrecht, NETHERLANDS.

P109VALIDATION OF GENOTYPING METHOD FOR HUMAN LEUKOCYTE ANTIGEN BASED ON NEXT GENERATION SEQUENCING TECHNOLOGYMing Li1, Michael Mindrinos1, Chunlin Wang1, Elaine Drainas1, Farbod Babrzadeh1, Marcelo Fernandez Vina2, Emmanuel Mignot2, Hanna Ollila2, Douglas Levinson2, 1Immucor, Mountain View, CA, 2Stanford University School of Medicine, Palo Alto, CA.

P110RESOLUTION POTENTIAL OF KIR TYPING BASED ON HIGH-THROUGHPUT NGS GENOTYPING DATAInes Wagner1, Gerhard Schoefl1, Bianca Schoene1, Alexander H. Schmidt2,1, Vinzenz Lange1, 1DKMS Life Science Lab GmbH, Dresden, GERMANY, 2DKMS German Bone Marrow Donor Center, Tübingen, GERMANY.

P111FIVE LOCUS HIGH RESOLUTION EXTENDED HAPLOTYPES (EH) IN HEALTHY MEXICAN MESTIZOS FROM THE HIGHLANDS OF MEXICOHilario Flores-A1,2, Andrea Munguía1, Araceli Rodríguez-Gómez1, Aida Delgado1,2, Betsy González1,2, Enrique Bonilla-Galán1, Miriam Valencia1, Felicitas Manzanarez1, Clara Gorodezky1,2, 1Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Department of Immunology and Immunogenetics, CDMX, México, MEXICO, 2Fundacion Comparte Vida A. C, Mexico City, MEXICO.

P112ANALYSIS OF KAS IMPACT ON A SINGLE CENTERAndrew J. Eckstein, Karl P. Schillinger, Julie A. Houp, Annette M. Jackson, Johns Hopkins University, Medicine, Baltimore, MD.

P113PREDICTIVE VALUE OF C1Q DONOR SPECIFIC ANTIBODY IN IDENTIFYING PATHOLOGIC FEATURES OF AMRElizabeth H. Field1,2, Sarat Kuppachi1, Diana Zepada-Orozco3, Danniele G. Holanda4, 1University of Iowa, Internal Medicine, Iowa City, IA, 2Veteran Affairs Medical Center, Iowa City, IA, 3University of Iowa, Pediatrics, Iowa City, IA, 4University of Iowa, Pathology, Iowa City, IA.


ABSTRACTSP114VALIDATION OF HIGH RESOLUTION HLA TYPING USING NXTYPETM ION-TORRENT NEXT GENERATION SEQUENCING. THE UNIVERSITY OF MIAMI EXPERIENCEDania Mateu, Emilio Margolles-Clark, Ana Hernandez, Phillip Ruiz, University of Miami, Surgery. Transplant Laboratory, Miami, FL.

P115FREQUENCIES OF RECOMBINATION IN THE HLA CLASS I & II REGIONS IN HEMATOPOIETIC STEM CELL TRANSPLANT PATIENTS AND RELATED DONORSHemantkumar Patel1, Yudith Carmazzi1, Titus Barnes1, Pedro Cano2, Marcelo Fernandez-Vina3, Kai Cao1, 1UT MD Anderson Cancer Center, Laboratory Medicine - HLA Typing Laboratory, Houston, TX, 2Moffitt Cancer Center, Tampa, FL, 3Stanford Medical School Blood Center, Palo Alto, CA.

P116IMMUNE RECONSTITUTION IN HEMATOPOIETIC STEM CELL TRANSPLANTED PATIENTS (HSCT): ASSESSING IMMUNE CELL SUBSETS AND CHIMERISMS AT DIFFERENT PERIODS POST-TRANSPLANTElizabeth García-G1, Alejandra Vázquez-A2, Luis M. Valero-S3, Brenda Acosta-M3, Araceli Rodriguez-G2, Eucario León-R4, Alberto Olaya-V5, Clara Gorodezky2,1, 1Fundación Comparte Vida, A.C., CDMX, México, MEXICO, 2Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Department of Immunology & Immunogenetics, CDMX, MEXICO, 3Instituto Nacional de Cancerología, Secretary of Health, Unit of Hematopoietic Stem Cell Transplantation, CDMX, MEXICO, 4Instituto Nacional de Ciencias Médicas y Nutrición, Secretary of Health, Hematopoietic Stem Cell Program, CDMX, MEXICO, 5Instituto Nacional de Pediatría, Secretary of Health, Unit of Hematopoietic Stem Cell Transplantation, CDMX, MEXICO.

P117SWAPPING THE SWAB FOR BMT CONFIRMATORY TYPINGLaurie Wakefield, Cynthia Kroning, Nicole Henderson, Brittany Schneider, Justin Kreuter, M Gandhi, Mayo Clinic, Department of Labortory Medicine & Pathology, Rochester, MN.

P118ASSOCIATION BETWEEN THE FREQUENCY OF HUMAN LEUKOCYTE ANTIGEN CLASS I AND CLASS II GENES IN SAUDI POPULATION AND RELATIVE RISK OF DEVELOPING NASOPHARYNGEAL CARCINOMAAziz A. Chentoufi1, Khalid A. AlZahrani1, Eman A. Mulia1, Latifah AlMakoshi2, Raneem


ABSTRACTSAlshareef2, Maria R. Paz1, Mehmet Dorak3, Nourhan Salloum1, Basmah AlGharbi1, Saleh AlDhahri2, 1King Fahad Medical City, Immunology, riyadh, SAUDI ARABIA, 2King Fahad Medical City, Head and Neck Department, riyadh, SAUDI ARABIA, 3Liverpool Hope University, School of Health Sciences, Liverpool, UNITED KINGDOM.

P119SINGLE-CENTER EXPERIENCE IN ROUTINE POST-TRANSPLANT ANTIBODY TESTING OF KIDNEY TRANSPLANT RECIPIENTS WITHOUT PRE-EXISTING DONOR SPECIFIC ANTI-HLA ANTIBODIESJ. Ryan Peña1, Francesca Cardarelli2, Eliyahu Khankin2, Martha Pavlakis2, 1Beth Israel Deaconess Medical Center, Pathology, Boston, MA, 2Beth Israel Deaconess Medical Center, Medicine, Boston, MA.

P120CORRELATION BETWEEN FLOWPRA® AND LABSCREEN CLASS II SINGLE ANTIGEN BEADS FOR THE DETECTION OF HLA DQB ANTIBODIES: NOT ALL ASSAYS ARE CREATED EQUAL.Patricia Brannon, Nathaniel Sutherland, Nalaja Marcus-Freeman, Howard Gebel, Robert Bray, Emory University Hospital, Atlanta, GA.

P121DRMohammed AlBalwi1, Mariam Ballow2, Abdulrahman AlAsiri2, Abdulkareem AlAbdulrahman2, Mohammed Aldrees2, Ibrahim AlAbdulkareem2, Ali H. Hajeer3, 1KAMC, Pathology & Lab Medicine, Riyadh, SAUDI ARABIA, 2King Abdullah International Medical Research Center, Medical Genomics Research Department, Riyadh, SAUDI ARABIA, 3King Abdulaziz Medical City, Pathology & Lab Medicine, Riyadh, SAUDI ARABIA.

P122RAPID ISOLATION OF CD15 POSITIVELY SELECTED CELLS FROM BLOOD FOLLOWING USE OF THE NEW EASYSEPTM RBC DEPLETION REAGENTKarina L. McQueen1, Jason W. Dixon1, Taylor N. Lee1, Carrie E. Peters1, Allen C. Eaves2,1, Terry E. Thomas1, 1STEMCELL Technologies, Research and Development, Vanncouver, BC, CANADA, 2BC Cancer Agency, Terry Fox Laboratory, Vanncouver, BC, CANADA.

P123HPA TYPING WITH LINKSĒQ™, A REAL-TIME PCR DETECTION SYSTEMZachary Antovich1, Thierry Viard1, Roland Russnak1, Queenie Ko1, Komal Singh1, Jonathan Downing2, Hans O. Madsen3, Gayle Teramura4, Sue Bowman5, Purvesh Patel6, 1Linkage Biosciences, Research and Development, South San Francisco,


ABSTRACTSCA, 2New Zealand Blood Service, Auckland, NEW ZEALAND, 3Rigshospitalet, Dept. Clin. Immunology, Copenhagen, DENMARK, 4Bloodworks Northwest, Specialty Diagnostics Labs, Seattle, WA, 5Australian Red Cross Blood Service, Transplantation & Immunogenetics Service, Melbourne, AUSTRALIA, 6Australian Red Cross Blood Service, Transplantation & Immunogenetics Service, Sydney, AUSTRALIA.

P124LINKSĒQ™ WIPE TEST, A REAL-TIME PCR DETECTION SYSTEM FOR AMPLIFICATION PRODUCTS CONTAMINATIONZachary Antovich1, Ngoc Ly2, Thierry Viard1, Komal Singh1, 1Linkage Biosciences, Research and Development, South San Francisco, CA, 2Linkage Biosciences, Manufacturing Operations, South San Francisco, CA.

P125HOW CAN WE DEVELOP THE BEST TEST ALGORITHM TO MONITOR HLA ANTIBODIES FOR RENAL RECIPIENTS? - AN EVALUATION OF SOLID PHASE ANTIBODY ASSAYSEileen S. MacCann1, Lorita M. Rebellato2, 1Vidant Medical Center, Pathology & Laboratory Medicine, Greenville, NC, 2The Brody School of Medicine at ECU, Pathology & Laboratory Medicine, Greenville, NC.

P126EXRESSION OF KILLER CELL IMMUNOGLOBULIN LIKE RECEPTORS AMONG ARABS FROM THE KUWAIT NATIONAL STEM CELL REGISTYReem Ameen1, Salem Al Shemmari1, Steven Marsh2, 1Health Sciences Center Kuwait University, Jabriya, KUWAIT, 2Antony Nolan Research, Institute and University College London Cancer Institute, London, UK, UNITED KINGDOM.

P127DETECTION OF MICA ANTIBODIES: COMPARISON BETWEEN MICA SCREENING AND SINGLE ANTIGEN TESTSXiaohai (Sam) Zhang1, Nancy Reinsmoen2, 1Cedars-Sinai Medical Center, Los Angeles, CA, 2Cedars-Sinai Health Systems, Los Angeles, CA.

P128TRANSPLANT PATIENTS WITH HLA ALLELE ANTIBODIES NEED DONORS TYPED AT THAT LEVELSteven S. Geier, Temple University Hospital and Lewis Katz scool of Medicine, Pathology and Laboratory Medicine, Philadelphia, PA.


ABSTRACTSP129MISSING THE FOREST FOR THE TREES: USING SHARED EPITOPE ANALYSIS TOOLS TO UNDERSTAND CROSSMATCH RESULTSAn T. Thai, Dong Li, Olga Timofeeva, Sandra Rosen-Bronson, Georgetown University Hospital, Histocompatibility, Washington, DC.

P130MELONTM GEL IGG SPIN PURIFICATION KIT: A ROBUST AND RAPID METHOD TO REMOVE ALL INTERFERING FACTORS FROM SERUMDenise E. Kielek, Renato M. Vega, Donna P. Lucas, Annette Jackson, Johns Hopkins University, Medicine, Baltimore, MD.

P131LIMITATIONS OF VIRTUAL CROSSMATCH FOR DECEASED KIDNEY TRANSPLANTATION IN HIGHLY SENSITIZED PATIENTSVivek Jani1, Barry Falch2, Chunlong Yang2, Gerald Morris2, 1University of California San Diego, San Diego, CA, 2University of California San Diego, Department of Pathology, San Diego, CA.

P132COMPLETE NUCLEOTIDE SEQUENCE CHARACTERIZATION OF DRB5 ALLELES INDICATE A HOMOGENEOUS ALLELE GROUP WHICH IS DISTINCT FROM OTHER DRB GENESKonstantinos Barsakis, Farbod Babrzadeh, Anjo Chi, Michael N. Mindrinos, Marcelo A. Fernandez Vinã, Stanford Blood Center, School of Medicine, Palo Alto, CA.

P133C1Q SINGLE ANTIGEN BEAD TESTING: A CASE WITH QUESTIONABLE SIGNIFICANCE?Kevin Burns1,2,3, Tammi Whitted1, James C. Cicciarelli1,4,5,6, Nathan A. Lemp4,5, Ellen Klohe7, Youngil Chang4,5,8, 1BloodSource, Mather, CA, 2UCDavis School of Medicine, Sacramento, CA, 3CNSU College of Medicine, Elk Grove, CA, 4ViraCor-IBT Laboratories, Los Angeles, CA, 5MNIT Foundation, Los Angeles, CA, 6USC Keck School of Medicine, Los Angeles, CA, 7Inland Northwest Blood Center, Spokane, WA, 8Western University of Health Sciences, Pamona, CA.

P134FAST DE NOVO DONOR SPECIFIC ANTIBODY DEVELOPMENT IN LUNG TRANSPLANT RECIPIENTAndrew L. Lobashevsky1, Kevin M. Rosner1, John May1, David Roe2, Michael Duncan2,


ABSTRACTSChadi Hage2, Erin Lushin3, Eve Anderson3, 1Indiana University Health, Methodist Hospital, Histocompatibility Laboratory, Imdianapolis, IN, 2Indiana University Health, Thoracic Transplant, Imdianapolis, IN, 3Indiana University Health, Thoracic Transplant Pharmacy, Imdianapolis, IN.

P135DETERMINATION OF THE WHOLE HLA-DQB1*06:37 SEQUENCE BY THE COMBINATION OF LONG RANGE POLYMERASE CHAIN REACTION, NEXT GENERATION SEQUENCING AND PHASINGMarco Schäfer1, Wolfgang Peter1, Anna-Lena Hansen1, Uwe Kordes2, Hartmut Kabisch2, Peter Kühnl3, Thomas H. Eiermann3, Reinhard Kelsch4, Thomas M. Binder3, 1Stefan-Morsch-Foundation, HLA Laboratory, Birkenfeld, GERMANY, 2University Medical Center Hamburg-Eppendorf, Paediatric Haematology and Oncology, Hamburg, GERMANY, 3University Medical Center Hamburg-Eppendorf, HLA Laboratory, Hamburg, GERMANY, 4University Clinics of Münster, HLA Laboratory, Münster, GERMANY.

P136DE NOVO HLA ANTIBODIES WITH SIMILAR SPECIFICITIES IN THREE RECIPIENTS FROM THE SAME DECEASED ORGAN DONORMelanny Hidajat1, Robin Vos2, Dirk Kuypers3, Maarten Naesens3, Jacques Pirenne4, Liesbeth Daniel1, Marie-Paule Emonds1, 1Red Cross Flanders Belgium, HILA, Laboratory for Histocompatibility & Immunogenetics, Mechelen, BELGIUM, 2University Hospital Leuven, Lung Transplantation Unit, Leuven, BELGIUM, 3University Hospital Leuven, Nephrology and Renal Transplantation, Leuven, BELGIUM, 4University Hospital Leuven, Transplantation Surgery, Leuven, BELGIUM.

P137THE IMPORTANCE OF RE-EVALUATING THE HLA ANTIBODY PROFILE AND AUTO-ANTIBODY STATUS WHEN THE FLOW CYTOMETRY CROSSMATCH RESULT IS NOT AS EXPECTEDBlanca Ponce-Ngo1, Jiali Shen1, Marzanna Krolik1, Scott Hodgson1, Angelo N. Arnold1,2, 1Westchester Medical Center, Transplant Immunogenetics Laboratory, Valhalla, NY, 2New York Medical College School of Medicine, Pathology, Valhalla, NY.

P138MODULATION OF CD20 EXPRESSION ON B CELLS BY ANTI-CD20 ANTIBODIESElaine C. Bellintani, Renato de Marco, Renata Fantini, Tiago Valim, Denise Macedo, Yuriko Miyamoto, Julia Temin, Maria Gerbase-DeLima, AFIP, Immunogenetics, São Paulo, BRAZIL.


ABSTRACTSP139FALSE HOMOZYGOSITY OBSERVED FOR ONE SAMPLE’S HLA GENOTYPING ON TWO INDEPENDENT NEXT GENERATION SEQUENCING PLATFORMSZahra Kashi, Meagan Barner, Jenefer Dekoning, Gabriel Caceres, RaeAnna Neville, Russ Martin, Kashi Clinical Laboratories, Inc., HLA, Portland, OR.

P140SUCCESSFUL KIDNEY TRANSPLANTATION OF A HIGHLY SENSITIZED PATIENT (HSP) WITH ALLELE-SPECIFIC ANTIBODY (ASA) AGAINST DR52 AFTER SEVERAL REJECTED NATIONAL OFFERS: A CASE REPORTAbubaker M. Sidahmed, Noureddine Berka, University of Calgary, Department of Pathology and Laboratory Medicine and Calgary Laboratory Service, Calgary, AB, CANADA.

P141CDC-T-AHG POSITIVE, FLOW NEGATIVE CROSSMATCH DUE TO HLA-B57 SPECIFIC IGM ANTIBODY: CASE STUDYDana L. McLendon, Olga Menninger, Mengyang Lei, Gansuvd Balgansuren, Seattle Cancer Care Alliance, Clinical Immunogenetics Laboratory, Seattle, WA.

P142HYPERACUTE REJECTION IN A KIDNEY TRANSPLANT RECIPIENT WITH NO HLA ANTIBODIESNathan A. Lemp1,2, James C. Cicciarelli1,2,3, Michael N. Koss1,2,3, Tariq Shah4,5, Michiko Taniguchi6, Kevin M. Burns7,8,9, Philip M. Carpenter3, Noriyuki Kasahara1,2, Robert Naraghi4,5, 1Viracor-IBT Laboratories, Los Angeles, CA, 2MNIT Foundation, Los Angeles, CA, 3USC Keck School of Medicine, Los Angeles, CA, 4St. Vincent Medical Center, Multi-Organ Transplant Center, Los Angeles, CA, 5Transplant Research Institute, Los Angeles, CA, 6City of Hope National Medical Center, Duarte, CA, 7BloodSource, Mather, CA, 8UC Davis School of Medicine, Sacramento, CA, 9CNSU College of Medicine, Elk Grove, CA.

P143DETERMINING THE SEROLOGIC EQUIVALENT OF A RELATIVELY RARE COMMON ALLELE DRB1*11:17Elise MacLeod1, Robert Bresler1, Taba Kheradmand2, Sharon Skorupski3, John Gerlach4,1, Sam Ho2,1, 1Mid-America Transplant, St. Louis, MO, 2Gift of Life Michigan, Ann Arbor, MI, 3Henry Ford Hospital, Transplant Immunology Laboratory, Detroit, MI, 4Michigan State University, Immunohematology and Serology Laboratory, East Lansing, MI.


ABSTRACTSP144THINK OUTSIDE THE ‘THREE’: A CASE OF ALLO-SENSITIZATION FROM AN ALTERNATE ROUTEDeanna Manna, Luis Hidalgo, Patricia Campbell, Alberta Health Services, Edmonton, AB, CANADA.

P145THE PRESENCE OF HLA-IGM DONOR SPECIFIC ANTIBODIES AND POSSIBLE ASSOCIATION WITH ANTIBODY MEDIATED REJECTIONEmma Carreiro1, Narelle Watson1, Lyanne Weston1, Frederika Abou-Daher1, Rhonda Holdsworth2, 1Australian Red Cross Blood Service, Transplantation & Immunogenetics, Sydney, AUSTRALIA, 2Australian Red Cross Blood Service, Transplantation & Immunogenetics, Melbourne, AUSTRALIA.

P146THE USE OF SURROGATE CROSSMATCHING ALLOWS FOR THE DETECTION OF A POSSIBLE DONOR SPECIFIC ANTIBODY TO AN HLA-DQ HETERODIMERRebecca L. Upchurch, Sana A. Ramahi, James R. Meade, Nicholas K. Brown, Jerome G. Weidner, Susana Marino, University of Chicago Medicine, Transplant Immunology and Immunogenetics Laboratory, Chicago, IL.

P147UNEXPECTED THINGS FLOW CROSSMATCH CAN REVEALTaba Kheradmand, Kyle Putnam, Katrina Tanner, Sam Ho, Gift of Life Michigan, Ann Arbor, MI.

P148UNCOVERING ANTIBODIES TO CRYPTIC EPITOPES: USE OF FLOW CYTOMETRY CROSSMATCH TO DIFFERENTIATE BETWEEN FUNCTIONAL AND DENATURED EPITOPES.Massimo Mangiola1, Jon Lomago1, Marrari Marilyn2, Lynn Nichol1, Dwayne Zern1, Kim McGowan3, Doreen Sese3, Laurine Bow3, Adriana Zeevi1, 1UPMC, Division of Transplant Pathology, Pittsburgh, PA, 2University of Pittsburgh, Department of Pathology, Pittsburgh, PA, 3Yale University, New Haven, CT.

P149SUCCESSFUL PEDIATRIC HEART TRANSPLANT ACROSS LOW TITER-C1Q-NEGATIVE DSA AND A DISCORDANT FLOW/CDC CROSSMATCH RESULTS AFTER BORTEZOMIB THERAPY.Massimo Mangiola1, Brian Feingold2, Lomago John1, Betty Hunter1, Carol Curry1, Larry Jelinek1, Lynn Nichol1, Susan Miller2, Matt Zinn2, Shawn West2, Michael A. McCulloch3,


ABSTRACTSAdriana Zeevi1, 1UPMC, Division of Transplant Pathology, Pittsburgh, PA, 2CHP, Cardiology, Pittsburgh, PA, 3Nemours/Alfred I. duPont Hospital for Children, Cardiology, Willmington, DE.

P150A CASE OF A SHARED EPITOPEReut Hod Dvorai, Nancy D. Herrera, Ashley M. Ruiz, Anat R. Tambur, Northwestern University, Comprehensive Transplant Center, Chicago, IL.

P151ACUTE ANTIBODY-MEDIATED KIDNEY REJECTION ASSOCIATED WITH ANTI-BW6 ORIGINATED FROM ALLOSENSITIZATION TO HLA-C14 DURING PREGNANCY: A CASE REPORTStephen P. Persaud1, Thalachallour Mohanakumar2, Rowena Delos Santos3, Chang Liu1, 1Washington University School of Medicine, Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, St. Louis, MO, 2Washington University School of Medicine, Department of Surgery, Saint Louis, MO, 3Washington University School of Medicine, Department of Medicine, Division of Nephrology, Saint Louis, MO.

P152PROZONE PHENOMENA MAY SKEW THE STRENGTH OF DONOR SPECIFIC ANTIBODY (DSA)Fadi Al Zayer, Maha Al Harbi, Sahar Sandooqah, Tariq Ali, Ammar Abdulbaki, Hassan AlEid, Moheeb Al-Awwami, King Faisal Specialist Hospital and Research Center, Riyadh, SAUDI ARABIA.

P153DONOR SPECIFIC ANTI-HLA ANTIBODIES IN HAPLO-TRANSPLANT- WHAT TO TELL THE CLINICIAN?Manish J. Gandhi, Laurie Wakefield, Ajoy Dias, Cory Blixt, Theresa Jacobsen, Micah Zuccarelli, Rachel Barnes, Kristen Hogarth, Justin Kreuter, Mayo Clinic, Rochester, MN.

P154THE QUEST FOR RESOLVING A MYSTERYEdward Guerrero, Nathaniel Smith, Kai Cao, UT MD Anderson Cancer Center, Laboratory Medicine - HLA Typing Laboratory, Houston, TX.

P155CHALLENGES IDENTIFYING ANTIBODIES USING SOLID PHASE METHODS WHEN CONTROLS FAILElizabeth A. Portwood, Mark Ireton, Paul A. Brailey, Alin L. Girnita, Hoxworth Blood Center, Transplantation Immunology Division, Cincinnati, OH.


ABSTRACTSP156AN UNEXPECTED DISCOVERY COMPLICATES AN OTHERWISE UNEVENTFUL HLA IDENTICAL HEMAPOETIC STEM CELL TRANSPLANTJennifer L. Metz, Suraya Berger, Renato M. Vega, Christopher Avergas, Annette Jackson, Maria Bettinotti, Johns Hopkins University, Medicine, Baltimore, MD.

P157ACCELERATED GRAFT LOSS DUE TO NON-COMPLEMENT FIXING DONOR-SPECIFIC ANTIBODY AGAINST A SINGLE DP EPITOPEAmber Carriker1, Nicholas Dyson1, Mimi Cathers1, John Lunz2, Bradford West3, Marc Garfinkel3, Sam Ho1,4, 1Memorial Medical Center, Transplant Laboratory, Springfield, IL, 2Gift of Hope Organ & Tissue Donor Network, Histocompatibility & Immunogenetics Laboratory, Itasca, IL, 3Memorial Medical Center, The Alan G. Birtch, MD, Center for Transplant Services, Springfield, IL, 4Gift of Life Michigan, Ann Arbor, MI.

P158COULD CHIMERISM TESTING ON ORTHOTOPIC LIVER TRANSPLANT PATIENTS LEAD TO EARLIER GRAFT VERSUS HOST DISEASE INTERVENTION AND IN TURN MORE EFFECTIVE OUTCOMES?Annette Rearick1, Iyore James2, Sylvester Black2, Hemant Parekh1, 1The Ohio State University Wexner Medical Center, Tissue Typing Lab, Columbus, OH, 2The Ohio State University Wexner Medical Center, Surgery/Transplant, Columbus, OH.

P159HIGH-RESOLUTION HLA-TYPING CAN CONFIRM DIAGNOSIS OF GRAFT VERSUS HOST DISEASE AFTER ORTHOTOPIC LIVER-TRANSPLANTATIONSoumya Pandey, Bobbie Rhodes-Clark, Daniel Borja-Cacho, Yogesh Jethava, Terry Harville, University of Arkansas for Medical Sciences, Little Rock, AR.

P160IDENTIFICATION OF A NOVEL HLA DRB1*11 ALLELE AND HAPLOTYPE LACKING THE HLA-DRB3 GENENatasha M. Howard, Cindy Faust, John L. Schmitz, Eric T. Weimer, UNC Healthcare, Histocompatibility Lab, Chapel Hill, NC.


ABSTRACTSP161THE ROAD LESS TRAVELED: CALLING A NOVEL HLA-DPB1 ALLELE AGAINST THE ASHI PROFICIENCY TESTING CONSENSUSAmanda Willis, Shawna Kennedy, Jenifer Williams, Seung Kang, Lesley Kresie, Geoffrey Land, Medhat Askar, Baylor University Medical Center, Dallas, TX.

P162UNEXPECTED POSITIVE FLOW CROSSMATCH WITH A DECEASED DONOR ORGAN: TIMING OF AUTOLOGOUS FLOW CROSSMATCH?Justin Kreuter, Lisa Hallaway, Laurie Wakefield, Manish Gandhi, Mayo Clinic, Rochester, MN.

P163HLA TYPING FOR A RARE CASE WITH DISSEMINATED NONTUBERCULOUS MYCOBACTERIAL INFECTIONSJaishree Patel, Brian Nortan, Christine Braun, Zeying Du, Loyola University Medical Center, Pathology, Maywood, IL.

P164“REVERSE” TRANSFUSION RELATED ACUTE LUNG INJURY?: A COMPLICATED GRANULOCYTE TRANSFUSION CASE REPORTKevin Burns1,2,3, Grace Monis1,2, Sarah Barnhard2, Hanne Jensen2, Mehrdad Abedi2, Smaroula Dilioglou4, 1BloodSource, Mather, CA, 2UC Davis School of Medicine, Sacramento, CA, 3CNSU College of Medicine, Elk Grove, CA, 4Sanford Health, Fargo, ND.


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2016.ASHI-HLA...covering HLA haploidentical transplant and permissible mismatches. 4. Novel treatment approaches of allograft rejection targeting B cells, plasma cells, and complement