Embed Size (px)
Citation preview
BD Falcon™ Cell Culture Multi-FlaskMultiply your cell growth. Multiply your productivity.
Features
• Evendistributionofmediaacrossalllayersforhomogeneouscellgrowth.
• AbilitytomixcellsandreagentsintheBDFalconMulti-Flasksavestimeandreducesriskofcontamination.
• Flexibledesignletsyoupouroraspirate/recovercellsusingapipet.
• Consistentsurfacetreatmentsforpredictablescale-up.
• Lotnumberisprintedoneachflaskfortraceability.
• ManufacturedincompliancetocGMPstandards.
Designed to Fit Your Protocol
Simplify your scale-up. New BD Falcon Multi-Flasks offer the same footprint and the same reagent volumes and cell seeding densities per unit area as 175 cm² flasks. Plus, you’ll be using the same proven surface treatment as all other BD Falcon flasks.
Improves Your Cell Culture Productivity
BD Falcon Multi-Flasks deliver a thoughtful design that simplifies your workflow. You can eliminate multiple steps and reduce the risk of contamination.
• Pipet access allows you to aspirate to replenish media and recover cells without pouring.
• The mixing port enables rapid mixing inside the vessel and allows you to add your cell suspension, transfection, or other reagents directly to the flask. The port also enables uniform distribution of media and cells to facilitate homogeneous cell growth on all layers.
More Consistent Cell Growth
BD Falcon Multi-Flasks’ even distribution of media, proven vacuum-gas tissue culture surface treatment, and effective gas exchange all combine to provide an optimal cell culture environment. The result is high cell yield and a homogeneous cell population.
Visit bdbiosciences.com/multiflask for more information.
BDFalcon™CellCultureMulti-Flasksenableyoutogrowmorecellsfasterandeasier,therebymakingyourcellcultureworkflowmoreproductive.
Tissueculture-treatedBDFalconMulti-Flasksareavailablein3-or5-layerformatsandprovide525cm2or875cm2cellgrowthsurfacearea.Theycanbeusedwithawiderangeofliquidvolumes(upto50ml/layer).
EcoPack is a trademark of Clontech Laboratories, Inc. MSCGM is a trademark of Lonza Cologne GmbH.BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2011 BDBN0823114
BD Falcon Cell Culture Multi-FlaskMultiplyyourcellgrowth.Multiplyyourproductivity.
3-LAYER TISSUE CULTURE-TREATED, 525 CM2 2 12 1482695
5-LAYER TISSUE CULTURE-TREATED, 875 CM2 1 8 1482696
ORDER INFORMATIONBDFalconCellCultureMulti-Flask
ACCESSORIES
For technical assistance, contact BD Biosciences Technical Support at:
tel: 877.232.8995 or 978.901.7389
Description Qty./Pack Qty./Case Cat. No.
Description Qty./Pack Qty./Case Cat. No.
BD Falcon Pipets
BD Falcon Tubes
BD Falcon Flasks
ASPIRATING PIPET 2 ML INDIVIDUALLY WRAPPED
50 ML POLYPROPYLENE CONICAL TUBE
To see the complete line of BD Falcon Flasks, visit www.bdbiosciences.com
175 ML POLYPROPYLENE CONICAL TUBE
225 ML POLYPROPYLENE CONICAL TUBE
ASPIRATING PIPET 5 ML INDIVIDUALLY WRAPPED
10 ML INDIVIDUALLY WRAPPED PAPER-PLASTIC
10 ML INDIVIDUALLY WRAPPED PLASTIC-PLASTIC
SEROLOGICAL PIPET 25 ML INDIVIDUALLY WRAPPED
SEROLOGICAL PIPET 100 ML INDIVIDUALLY WRAPPED
50 200 13-675-16
50 200 13-675-10CC
50 200 13-675-20
50 200 13-675-49
50 200 13-675-50
5 50 13-675-73
25 500 14-432-22
8 48 05-538-62
8 48 05-538-61
PRODUCT SPECIFICATIONS>5 ml per layer for dissociating
<50 ml per layer for cell expansion
0 to 50 ml per layer in 10 ml increments
10%
0.2 μm hydrophobic membrane
Tissue culture-treated, optically clear
WORKING VOLUME RANGE
MOLDED-IN GRADUATIONS
GRADUATION ACCURACY
CAP VENT MEMBRANE
CELL GROWTH SURFACE
USAForcustomerservice,call1-800-766-7000Tofaxanorder,use1-800-926-1166Toorderonline:www.fishercsci.com
CANADAForcustomerservice,call1-800-234-7437Tofaxanorder,use1-800-463-2996Toorderonline:www.fishercsci.ca©2011 Thermo Fisher Scientific Inc. All rights reserved.
CONSISTENT CELL PHYSIOLOGY
CELL CULTURE VESSEL
T-175
5.0
4.0
3.0
2.0
1.0
0.0BD Falcon Multi-Flask (3-layer)
Cal
ciu
m M
ob
iliza
tio
nFo
ld In
crea
se O
ver
Co
ntr
ol
(mea
n ±
SD
)
CHO-M1cellsweregrowninaT-175anda3-layerBDFalconMulti-Flaskfor72hours.Cellswereharvestedandre-seededon96-wellPDLplatesforGPCRCalciummobilizationassay.CellswerechallengedwithM1agonist,carbachol(300µM)andresponseswererecorded1minutefollowingcompoundaddition.Dataisexpressedasfold-increaseincalciumfluxcomparedtocontrolcellschallengedwithassaybuffer(nodrug).Nosignificantdifferenceinagonistresponsewasob-servedbetweencellsharvestedfromeithervesseltype.Datashownisrepresentativeofthreeindependentexperiments.
PREDICTABLE SCALE-UP
Expected Yield Actual Yield T-175 BD Falcon Multi-Flask (3-layer) BD Falcon Multi-Flask (5-layer)
CELL CULTURE VESSEL CELL LINE
Cel
l Yie
ld (
x 10
6 ; mea
n ±
SD
)
Cel
ls p
er u
nit
are
a (x
106 ; c
ells
/cm
2 ) 0.5
0.4
0.3
0.2
0.1
0.0
300
250
200
150
100
50
0BD Falcon T-175 Flask
BHK-21 LnCap Hep-G2 EcoPack 2-293
BD Falcon Multi-Flask(3-layer)
BD Falcon Multi-Flask(5-layer)
ThreeandfivetimesthenumberofBHK-21cellsweregrownandrecoveredfrom3-and5-layerBDFalconMulti-FlaskscomparedtoT-175flasks.Expectedyield(leftpanel)wasdeterminedusingmeancellyieldfromcontrolT-175flasksmultipliedbythreeandfivetimesforthe3-and5-layerBDFalconMulti-Flasks,respectively.Cellyieldpercm2(rightpanel)wasequivalentin3-and5-layerBDMulti-FlasksandT-175flasksforBHK-21,LnCap,Hep-G2andEcoPack™2-293cells.
SIMPLIFIED WORKFLOW
• Pipetaccesseliminatestheneedforpouring.Italsofacilitatestheuseofawiderangeofliquidvolumes(upto50ml/layer);efficientrecoveryofvaluablecellsandreagents;andminimizestheriskofcontamination.
• Mixingporteliminatesmixingoutsidethevessel,savingyoutimeandeffort.Thisuniquein-vesselmixingallowsrapidanduniformdistributionofcellsandreagentsinalllayersoftheflasks.
• CompatibilitywithsimilarvolumesofreagentusageandcellseedingdensitiesasT-175flasksmakesscalingupafamiliarandsimpleprocess.
CONSISTENT CELL GROWTH
IDEAL FOR MULTIPOTENT STEM CELLS
BD T-175 (control)Top Middle Bottom
ThisfigureillustratesuniformcellgrowthbetweenlayersofBDFalconMulti-Flasks.BHK-21cellsgrownto>80%confluencein3-layerBDFalconMulti-FlasksandcontrolT-175flaskswerefixedandstainedwithcrystalviolet.ControlflasksandindividuallayersoftheBDFalconMulti-Flaskswerecutandscanned.
HumanMesenchymalStemCells(hMSCs)wereculturedinMSCGM™medium(Lonza)andseededat4,000to5,000cells/cm2inT-175flasksand3-layerBDFalconMulti-Flasks.Cellswerecultureduntiltheyreached80%confluence(~6days).Cellswereharvested,counted,andcumulativepopulationdoublingsofhMSCsfromeachvesseltypewerecalculatedoverfiveconsecutivepassages.ImmunophenotypicpropertiesofhMSCsgrowninBDFalconMulti-Flasksasdeterminedbyflowcytometricanalysis(BDFACS™)confirmedthathMSCswerenegativeforsurfacemarkersCD14,CD34,CD45,CD79a,andHLA-DRandpositiveforexpressionofsurfacemarkersCD73,CD90,andCD105.Respectiveisotypecontrolsforeachmarkerantibodyareshowningrey.
COMPATIBLE WITH YOUR CELL LINES
BD Falcon T-175 Flask BD Falcon Multi-Flask (3-layer)
CELL LINES
Cel
l Yie
ld (
x 10
6±
SD
)
250
200
150
100
50
0BHK-21 HUVEC-2 LnCap Hep-G2 EcoPack
2-293MSC
(+ Serum)MSC
(- Serum)CHO-M1
Diversecelllinesandprimarycultures(withandwithoutserum)canbegrownandefficientlyrecoveredfromBDFalconMulti-Flaskcellculturevessels.ThisgraphillustratestheincreasedcellyieldonecanobtainfromBDFalconMulti-FlasksascomparedtocontrolT-175flasksforvariouscelllines.
0
1
2
3
4
5
6
7
8
9
0 5 10 15 20 25 30 35
Me
an
Po
pu
lati
on
Do
ub
lin
gs
NUMBER OF DAYS IN CULTURE
T-175
BD 3-layer
CONSISTENT CELL PHYSIOLOGY
CELL CULTURE VESSEL
T-175
5.0
4.0
3.0
2.0
1.0
0.0BD Falcon Multi-Flask (3-layer)
Cal
ciu
m M
ob
iliza
tio
nFo
ld In
crea
se O
ver
Co
ntr
ol
(mea
n ±
SD
)
CHO-M1cellsweregrowninaT-175anda3-layerBDFalconMulti-Flaskfor72hours.Cellswereharvestedandre-seededon96-wellPDLplatesforGPCRCalciummobilizationassay.CellswerechallengedwithM1agonist,carbachol(300µM)andresponseswererecorded1minutefollowingcompoundaddition.Dataisexpressedasfold-increaseincalciumfluxcomparedtocontrolcellschallengedwithassaybuffer(nodrug).Nosignificantdifferenceinagonistresponsewasob-servedbetweencellsharvestedfromeithervesseltype.Datashownisrepresentativeofthreeindependentexperiments.
PREDICTABLE SCALE-UP
Expected Yield Actual Yield T-175 BD Falcon Multi-Flask (3-layer) BD Falcon Multi-Flask (5-layer)
CELL CULTURE VESSEL CELL LINE
Cel
l Yie
ld (
x 10
6 ; mea
n ±
SD
)
Cel
ls p
er u
nit
are
a (x
106 ; c
ells
/cm
2 ) 0.5
0.4
0.3
0.2
0.1
0.0
300
250
200
150
100
50
0BD Falcon T-175 Flask
BHK-21 LnCap Hep-G2 EcoPack 2-293
BD Falcon Multi-Flask(3-layer)
BD Falcon Multi-Flask(5-layer)
ThreeandfivetimesthenumberofBHK-21cellsweregrownandrecoveredfrom3-and5-layerBDFalconMulti-FlaskscomparedtoT-175flasks.Expectedyield(leftpanel)wasdeterminedusingmeancellyieldfromcontrolT-175flasksmultipliedbythreeandfivetimesforthe3-and5-layerBDFalconMulti-Flasks,respectively.Cellyieldpercm2(rightpanel)wasequivalentin3-and5-layerBDMulti-FlasksandT-175flasksforBHK-21,LnCap,Hep-G2andEcoPack™2-293cells.
SIMPLIFIED WORKFLOW
• Pipetaccesseliminatestheneedforpouring.Italsofacilitatestheuseofawiderangeofliquidvolumes(upto50ml/layer);efficientrecoveryofvaluablecellsandreagents;andminimizestheriskofcontamination.
• Mixingporteliminatesmixingoutsidethevessel,savingyoutimeandeffort.Thisuniquein-vesselmixingallowsrapidanduniformdistributionofcellsandreagentsinalllayersoftheflasks.
• CompatibilitywithsimilarvolumesofreagentusageandcellseedingdensitiesasT-175flasksmakesscalingupafamiliarandsimpleprocess.
CONSISTENT CELL GROWTH
IDEAL FOR MULTIPOTENT STEM CELLS
BD T-175 (control)Top Middle Bottom
ThisfigureillustratesuniformcellgrowthbetweenlayersofBDFalconMulti-Flasks.BHK-21cellsgrownto>80%confluencein3-layerBDFalconMulti-FlasksandcontrolT-175flaskswerefixedandstainedwithcrystalviolet.ControlflasksandindividuallayersoftheBDFalconMulti-Flaskswerecutandscanned.
HumanMesenchymalStemCells(hMSCs)wereculturedinMSCGM™medium(Lonza)andseededat4,000to5,000cells/cm2inT-175flasksand3-layerBDFalconMulti-Flasks.Cellswerecultureduntiltheyreached80%confluence(~6days).Cellswereharvested,counted,andcumulativepopulationdoublingsofhMSCsfromeachvesseltypewerecalculatedoverfiveconsecutivepassages.ImmunophenotypicpropertiesofhMSCsgrowninBDFalconMulti-Flasksasdeterminedbyflowcytometricanalysis(BDFACS™)confirmedthathMSCswerenegativeforsurfacemarkersCD14,CD34,CD45,CD79a,andHLA-DRandpositiveforexpressionofsurfacemarkersCD73,CD90,andCD105.Respectiveisotypecontrolsforeachmarkerantibodyareshowningrey.
COMPATIBLE WITH YOUR CELL LINES
BD Falcon T-175 Flask BD Falcon Multi-Flask (3-layer)
CELL LINES
Cel
l Yie
ld (
x 10
6±
SD
)
250
200
150
100
50
0BHK-21 HUVEC-2 LnCap Hep-G2 EcoPack
2-293MSC
(+ Serum)MSC
(- Serum)CHO-M1
Diversecelllinesandprimarycultures(withandwithoutserum)canbegrownandefficientlyrecoveredfromBDFalconMulti-Flaskcellculturevessels.ThisgraphillustratestheincreasedcellyieldonecanobtainfromBDFalconMulti-FlasksascomparedtocontrolT-175flasksforvariouscelllines.
0
1
2
3
4
5
6
7
8
9
0 5 10 15 20 25 30 35
Me
an
Po
pu
lati
on
Do
ub
lin
gs
NUMBER OF DAYS IN CULTURE
T-175
BD 3-layer
BD Falcon™ Cell Culture Multi-FlaskMultiply your cell growth. Multiply your productivity.
Features
• Evendistributionofmediaacrossalllayersforhomogeneouscellgrowth.
• AbilitytomixcellsandreagentsintheBDFalconMulti-Flasksavestimeandreducesriskofcontamination.
• Flexibledesignletsyoupouroraspirate/recovercellsusingapipet.
• Consistentsurfacetreatmentsforpredictablescale-up.
• Lotnumberisprintedoneachflaskfortraceability.
• ManufacturedincompliancetocGMPstandards.
Designed to Fit Your Protocol
Simplify your scale-up. New BD Falcon Multi-Flasks offer the same footprint and the same reagent volumes and cell seeding densities per unit area as 175 cm² flasks. Plus, you’ll be using the same proven surface treatment as all other BD Falcon flasks.
Improves Your Cell Culture Productivity
BD Falcon Multi-Flasks deliver a thoughtful design that simplifies your workflow. You can eliminate multiple steps and reduce the risk of contamination.
• Pipet access allows you to aspirate to replenish media and recover cells without pouring.
• The mixing port enables rapid mixing inside the vessel and allows you to add your cell suspension, transfection, or other reagents directly to the flask. The port also enables uniform distribution of media and cells to facilitate homogeneous cell growth on all layers.
More Consistent Cell Growth
BD Falcon Multi-Flasks’ even distribution of media, proven vacuum-gas tissue culture surface treatment, and effective gas exchange all combine to provide an optimal cell culture environment. The result is high cell yield and a homogeneous cell population.
Visit bdbiosciences.com/multiflask for more information.
BDFalcon™CellCultureMulti-Flasksenableyoutogrowmorecellsfasterandeasier,therebymakingyourcellcultureworkflowmoreproductive.
Tissueculture-treatedBDFalconMulti-Flasksareavailablein3-or5-layerformatsandprovide525cm2or875cm2cellgrowthsurfacearea.Theycanbeusedwithawiderangeofliquidvolumes(upto50ml/layer).
EcoPack is a trademark of Clontech Laboratories, Inc. MSCGM is a trademark of Lonza Cologne GmbH.BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2011 BDBN0823114
BD Falcon Cell Culture Multi-FlaskMultiplyyourcellgrowth.Multiplyyourproductivity.
3-LAYER TISSUE CULTURE-TREATED, 525 CM2 2 12 1482695
5-LAYER TISSUE CULTURE-TREATED, 875 CM2 1 8 1482696
ORDER INFORMATIONBDFalconCellCultureMulti-Flask
ACCESSORIES
For technical assistance, contact BD Biosciences Technical Support at:
tel: 877.232.8995 or 978.901.7389
Description Qty./Pack Qty./Case Cat. No.
Description Qty./Pack Qty./Case Cat. No.
BD Falcon Pipets
BD Falcon Tubes
BD Falcon Flasks
ASPIRATING PIPET 2 ML INDIVIDUALLY WRAPPED
50 ML POLYPROPYLENE CONICAL TUBE
To see the complete line of BD Falcon Flasks, visit www.bdbiosciences.com
175 ML POLYPROPYLENE CONICAL TUBE
225 ML POLYPROPYLENE CONICAL TUBE
ASPIRATING PIPET 5 ML INDIVIDUALLY WRAPPED
10 ML INDIVIDUALLY WRAPPED PAPER-PLASTIC
10 ML INDIVIDUALLY WRAPPED PLASTIC-PLASTIC
SEROLOGICAL PIPET 25 ML INDIVIDUALLY WRAPPED
SEROLOGICAL PIPET 100 ML INDIVIDUALLY WRAPPED
50 200 13-675-16
50 200 13-675-10CC
50 200 13-675-20
50 200 13-675-49
50 200 13-675-50
5 50 13-675-73
25 500 14-432-22
8 48 05-538-62
8 48 05-538-61
PRODUCT SPECIFICATIONS>5 ml per layer for dissociating
<50 ml per layer for cell expansion
0 to 50 ml per layer in 10 ml increments
10%
0.2 μm hydrophobic membrane
Tissue culture-treated, optically clear
WORKING VOLUME RANGE
MOLDED-IN GRADUATIONS
GRADUATION ACCURACY
CAP VENT MEMBRANE
CELL GROWTH SURFACE
USAForcustomerservice,call1-800-766-7000Tofaxanorder,use1-800-926-1166Toorderonline:www.fishercsci.com
CANADAForcustomerservice,call1-800-234-7437Tofaxanorder,use1-800-463-2996Toorderonline:www.fishercsci.ca©2011 Thermo Fisher Scientific Inc. All rights reserved.
