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金萬林公司2011年季末GE 蛋白質純化產品促銷
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Protein PurificationProtein Purification
0800-009-695
/////12841 :0800-0 09-695Mail:[email protected] TEL:02-2790-2222 FAX:02-27929986
5
-- HiScaleHiScale ColumnColumnXK
Simple manual purification of antibodiesSimple manual purification of antibodiesrProteinrProtein A/G A/G GraviTrapGraviTrap TMTM
Cubee 2~1.5ml tube x 8 / 0.5ml tube x 8 / 0.2ml tube x 167200rpm
HisHis--tagged protein purificationtagged protein purification
Ni Sepharose
XK or Spin column 5 /25/100ml ~ 40 mg/ml 90 Ni Sepharose 6 FF 17-5318-06 /-01/-02
XK, Tricorn or Spin column 25 / 100 ml ~ 40 mg/ml 34 Ni Sepharose HP 17-5268-01/-02
Quantity Binding capacity Particalesize (um) Product Code number
Metal Chelate Affinity
XK or Spin column 50 ml ~ 40 mg/ml 90 Chelating Sepharose 6 FF 17-0575-01
XK or Spin column 25/100 ml ~ 40 mg/ml 90 IMAC Sepharose 6 FF 17-0921-07/-08
XK, Tricorn or Spin column 25/100 ml ~ 40 mg/ml 34 IMAC Sepharose HP 17-0920-06 /-07
Quantity Binding capacity Particalesize (um) Product Code number
Pre-packed Columns
Anti-His Antibody
5x1/5x5ml23 umol Cu2+/ml medium34HisTrap Chelating HP17-0408-01/-0409-03
5x1/5x5ml> 40/200 mg His-tagged protein34HisTrap HP17-5247-01/-5248-02
5x1/5x5ml> 40/200 mg His-tagged protein34HisTrap IMAC HP17-0920-03/-0920-05
5x1/5x5ml> 40/200 mg His-tagged protein90HisTrap IMAC FF17-0921-02/-0921-04
5x1/5x5ml~ 40/200 mg His-tagged protein90HisTrap FF crude11-0004-58/-5286-01
170ul (2.6ug/ul)Anti-His Antiboddy27-4710-01
5x1/5x5ml ~ 10 / 50 recombinant GST 90 HisTrap FF17-5319-01/-5255-01
20 ml 800 mg His-tagged protein90 HisPrep FF 16/10 28-9365-51
10x 1 ml ~ 40 mg His-tagged protein 90 His GraviTrap11-0033-99
50x100 ul~750g His-tagged protein 34 His SpinTrap28-4013-53
Quantity Binding capacity Particalesize (um)
Product Code number
MBPMBP--& Strep (& Strep (llll))--taggedtagged
Dextrin Sepharose / StrepTactin Sepharose
Dextrin Sepharose
XK, Tricorn or Spin column 10 ml ~ 6 mg/ml 34 StrepTactin Sepharose HP 28-9355-99
XK, Tricorn or Spin column 25 ml 7~16 mg/ml 34 Dextrin Sepharose HP 28-9355-97
Quantity Binding capacity
Particalesize (um)
Product Code number
Strep Tactin Sepharose
Pre-packed Columns
HiTap column5x1/5x5ml~6/30 mg Strep(II)-tagged protein34StrepTrap HP28-9075-46/-9075-48
HiTap column 5x1/5x5ml~10/50 mg Strep(II)-tagged protein34 MBPTrap HP 28-9187-78/-9187-80
Quantity Binding capacity Particalesize (um) Product Code number
>tagged-protein Transition metals : Ni2+, Cu2+, Zn2+,Co2+, Fe2+, Fe3+, Cd2+and Hg2+
1.2.3.4.5.: : His GraviTrap/: HisTrap
Strep(ll) His-TagDual-tagMBPfolding
Cu2+ ,Zn2+, Co2+,Ni2+
GSTGST--tagged protein Systemtagged protein System
Cleavage enzymes Cleavage enzymes
Factor Xa protease: Mr 28~30 kD
Thrombin protease: Mr 37 kD
PreScission Protease: Mr 46 kD
XK or Spin column 10 ml > 5 mg/ml 90 Glutathione Sepharose 4B 17-0756-01
XK or Spin column 25 / 100ml ~ 10 mg/ml 90 Glutathione Sepharose 4 FF 17-5132-01 /-02
XK, Tricorn or Spin column 25 /100 ml ~ 10 mg/ml 34 Glutathione Sepharose HP 17-5279-01 /-02
Quantity Binding capacity
Particalesize (um)
Product Code number
pGEX Vectors products
Glutathione Sepharose
GST- Pre-packed Columns
Glutathione Sepharose
Cleavage site
Detection Anti-GST Antibody
0.5ml ,50 dectectionsAnti-GST antibody27-4577-01
HiTrap column5x1/5x5ml~ 10 / 50 recombinant GST34GSTrap HP17-5281-01/-5282-02
75ulAnti-GST HRP ConjugateRPN1236
HiTrap column5x1/5x5ml ~ 10 / 50 recombinant GST 90 GSTrap 4B28-4017-45/-4017-48
HiTrap column5x1/5x5ml ~ 10 / 50 recombinant GST 90 GSTrap FF 17-5130-01/-5131-02
HiPrep column 20 ml 200 recombinant GST 90 GSTPrep FF16/10 28-9365-50
Spin column 50 columns 400g GST-tagged protein 90 GST SpinTrap purification Modules 28-9523-59
Quantity Binding capacity Particalesize (um)Product Code number
New ! HiScaleXKadaptor)
XK columnadaptor) Pressure limit -- XK 16, XK 26 5 bar
C columnPressure limit 1 bar (14.5 psi, 0.1 MPa)
RC 10/16 reservoir
AC10/16/26 adaptor
C 26/100 column
C 26/70 column
C 26/40 column
C 16/70 column
C 16/40 column
C 16/20 column
C 10/40 column
C 10/20 column
C 10/10 column
Product
18-8793-01
Packing equipment
18-8778-01/-8766-01
Flow adaptor
28-9889-53
18-8770-01
28-9889-50
18-8768-01
28-9889-48
18-8776-01
28-9889-46
18-8774-01
18-8773-01
Code number
RK 16/26 reservoir
AK 16/26 adaptor
XK 50/30 column
XK 26/100 column
XK 26/70 column
XK 26/40 column
XK 26/20 column
XK 16/100 column
XK 16/70 column
XK 16/40 column
XK 16/20 column
Product
28-9645-03
Pack connector
28-9663-83/-84/-85
Flow adaptor
28-9644-44
28-9644-45
28-9645-13
28-9645-14
28-9644-24
28-9644-41
Code number
HiScale 16/26/50 adaptor
HiScale 50/40
HiScale 50/20
HiScale 26/40
HiScale 26/20
HiScale 16/40
HiScale 16/20
Product
19-5102-01
19-5103-01
19-5201-01
19-5202-01
19-5007-01 /-5110-01
Packing reservoir
19-5006-01 /-5109-01 /-5207-01
Flow adaptor
19-5103-01
19-5101-01
19-5003-01
19-5002-01
19-5001-01
Code number
10 LabMate PD-10 buffer reservoir 18-3216-03
50 Empty PD-10 columns 17-0435-01
Quantity Product Code number
Quantity Product Code number
25 ugpGEX-6P-1 28-9546-48
500 units PreScission Protease27-0843-01
25 ugpGEX-4T-1 28-9545-49
500 unitsThrombin protease27-0846-01
25 ugpGEX-5X-1 28-9545-53
400 unitsFactor Xa Protease27-0849-01
PD-10 & reservoir
XK column
HiScale column
Empty ColumnsEmpty Columns
Antibody purificationAntibody purificationSpecificity Guide
HiTrap columns
XK or Spin column 5/25 ml > 20 mg/ml 90 Protein G Sepharose 4 FF (for IP) 17-0618-01/-02
XK or Spin column 5/25 ml > 35 mg/ml 90 rmp Protein A Sepharose FF 17-5138-01/-02
XK or Spin column 25 ml ~ 20 mg/ml 90 Protein A Sepharose CL-4B 17-0963-03
XK or Spin column 1.5 g ~ 20 mg/ml 90 Protein A Sepharose CL-4B 17-0780-01
XK or Spin column 5 /25ml ~ 6 mg/ml 90 nProtein A Sepharose 4 FF (for IP) 17-5280-01/-04
XK or Spin column 5 /25ml ~ 6 mg/ml 90 rProtein A Sepharose FF 17-1279-01/-02
Spin column 2 x 2 ml Immunoprecipitation Starter Pack (for IP) 17-6002-35
Quantity Binding capacity Particalesize (um) Product Code number
Pre-packed Columns
GraviTrap coiumn10x1 ml~ 50 mg human IgG90rProtein A GravitTrap28-9852-54
GraviTrap coiumn10x1 ml~ 20 mg human IgG90Protein G GraviTrap28-9852-55
Spin column16 x 100l>2.5 mg human igG34Protein G HP SpinTrap28-9031-34
Spin column16 x 100l ~2 mg human igG34ProteinA HP SpinTrap28-9031-32
HiTrap column5x1/5x5ml25/125 mg human IgG34HiTrap Protein G HP17-0404-01/-0405-03
HiTrap column5x1ml5 mg human IgM34HiTrap IgM Punification HP17-5110-01
HiTrap column1x5ml100 mg IgY34HiTrap IgY Punification HP17-5111-01
HiTrap column5x1/5x5ml 20/100 mg human IgG34 HiTrap Protein A HP 17-0402-01/-0403-03
HiTrap column5x1/5x5ml50/250 mg human IgG90 HiTrap rProtein A FF 17-5079-01/-5080-02
Spin column 50x100l>1 mg IgG/ column 34 Ab SpinTrap28-4083-47
Quantity Binding capacity
Particalesize (um)
Product Code number
Protein A / Protein G Sepharose
IP
Hot
Hot
Mag SepharoseTM Xtra--
His buffer kit11-0034-00
Mag Rack 6 (28-9489-64
TiO2 Mag Sepharose28-9440-10/28-9513-771x0.5 /4x0.5 ml
NHS Mag Sepharose28-9440-09/28-9513-80
10x1 mlHis Mag Sepharose Ni28-9799-17
2x1 /5x1ml~ 50 mg histidine-tagged protein /ml
His Mag Sepharose Ni28-9673-88/-90
2x1 /5x1ml> 27 mg human IgG/ml gelProtein G Mag Sepharose Xtra28-9670-66/-70
2x1 /5x1ml > 27 mg human IgG/ml gelProtein A Mag Sepharose Xtra28-9670-56/-62
Quantity Binding capacity Product Code number
Capacity target protein non-adherentsmearingcapture target protein (g ~ mg)
Hot
IP
FPLC
HisTrapHisTrap ColumnColumn
NEWNEW
Affinity purificationAffinity purification
Coupling gel
XK or Spin column 15 g 90 Activated Thiol Sepharose 4B 17-0640-01
XK or Spin column 15 g 90 Activated CH Sepharose 4B 17-0490-01
XK or Spin column 15 g 90 CNBr-activated Sepharose 4B 17-0430-01
XK or Spin column 10 g 90 CNBr-activated Sepharose 4 FF 17-0981-01
XK or Spin column 25 ml 90 NHS-activated Sepharose 4 FF 17-0906-01
Quantity Particalesize (um)
Product Code number
spacer arm
Ligand spacer arm - (ligand) (Mr < 5,000) spacer arm
Group specific gel
NA
NA
6 mg/ml
>18 mg/ml
NA
NA
>35 mg/ml
Binding capacity
XK or Spin column 5/100ml90Con A Sepharose 4B17-0440-03/-01
XK or Spin column 25 ml90Lentil Lectin Sepharose 4B17-0444-01
XK or Spin column 50 ml90Blue Sepharose 6 FF17-0948-01
XK or Spin column Tricorn5 ml34Stretavidin Sepharose HP17-5113-01
XK or Spin column 10 ml90 IgG Sepharose 6 FF17-0969-01
XK or Spin column 10 ml 90 Calmodulin Sepharose 4B17-0529-01
XK or Spin column 25 ml 90 Benzamidine Sepharose 4 FF (high sub)
17-5123-10
Quantity Particalesize (um)
Product Code number
Pre-packed Columns
HiTrap column5x1 ml>300nmol biotin34HiTrap Streptavidin HP17-5112-01
Spin column16 x 100l >0.6 mg biotinylated BSA34Streptavidin HP SpinTrap28-9031-30
HiTrap column5x1/1x5mlApplication dependent34HiTrap NHS-activated HP17-0716-01/-0717-01
HiTrap column5x1/1x5ml20/>100mg HSA34HiTrap Blue HP17-0412-01/-0413-01
HiTrap column5x1/1x5ml>35/175 mg trypsin90HiTrap Benzamidine FF17-5143-01/-5144-01
HiTrap column5x1/5x5ml 3 / 15 mg antithrombin III34 HiTrap Heparin HP17-0406-01/-0407-03
HiPrep20 ml 40 mg antithrombin III90HiPrep 16/10 Heparin FF17-5189-01
Spin column2.5 mlFor phosphorylated peptide90 Phos SpinTrap Fe28-9298-81
Spin column 5 ml34 NHS HP SpinTrap28-9031-28
Quantity Binding capacity
Particalesize (um)
Product Code number
DesaltingDesalting Easy, small batch purification
Pre-packed Columns
Hot
Spin column50 x 500l 70~130ul85~260PD SpinTrap G-2528-9180-04
HiPrep column1x53/4x53ml
Ion Exchange protein purificationIon Exchange protein purification
Sepharose
Pre-packed Columns
HiPrep column20 ml2,200 mg HSA90HiPrep 16/10 DEAE FF17-5090-01
HiPrep column20 ml860 mg BSA90HiPrep 16/10 ANX FF(high sub)17-5191-01
HiPrep column20 ml2,400 mg HAS90HiPrep 16/10 Q FF17-5190-01
Tricorn column1 ml< 50 mg10Mono S 5/50 GL17-5168-01
RESOURCE column1 ml< 25 mg15RESOURCE S 1ml17-1178-01
RESOURCE column1 ml < 25 mg15 RESOURCE Q 1ml17-1177-01
HiLoad column20 ml 160 mg/ml 90 SP Sepharose XL 17-5073-01
XK column 300 ml >130 mg/ml 90 Q Sepharose XL 17-5072-01
XK column 25/500 ml 5 mg/ml 90 ANX Sepharose 4 FF (high sub) 17-1287-10 /-01
XK column 25/500 ml 50 mg/ml 90 CM Sepharose FF 17-0719-10/-01
XK column 25/300 ml 70 mg/ml 90 SP Sepharose FF 17-0729-10/-01
XK column 25/500 ml 110 mg/ml 90 DEAE Sepharose FF 17-0709-10/-01
XK column 25/300 ml 120 mg/ml 90 Q Sepharose FF 17-0510-10/-01
XK or Tricorn column 25/100 ml NA 75 Capto adhere NEW 17-5444-10/-01
XK or Tricorn column 25/100 ml >45 mg/ml 75 Capto MMC 17-5317-10/-02
XK or Tricorn column 25/100 ml >100 mg/ml 90 Capto ViralQ28-9032-30/-31
XK or Tricorn column 25/100 ml >120 mg/ml 90 Capto S 17-5441-10/-01
XK or Tricorn column 25/100 ml >90 mg/ml 90 Capto DEAE NEW 17-5443-10/-01
XK or Tricorn column 25/100 ml >100 mg/ml 90 Capto Q 17-5316-10/-02
XK or Tricorn column 50 ml NA 30 SOURCE 30S 17-1273-01
Tricorn column 10/50 ml NA 15 SOURCE 15S 17-0944-10/-01
XK or Tricorn column 50 ml NA 30 SOURCE 30Q 17-1275-01
Tricorn column 10/50 ml NA 15 SOURCE 15Q 17-0947-20/-01
Quantity Binding capacity Particalesize (um) Product Code number
Hot
Hydrophobic interaction protein purificationHydrophobic interaction protein purification
Pre-packed Columns
XK column 25/200 ml 27 mg/ml 90 Octyl Sepharose 4 FF 17-0946-10/-02
XK column 25/100 ml 7 mg/ml 90 Butyl Sepharose 4 FF 17-0980-10/-01
XK column 25/200 ml NA 90 Butyl-S Sepharose 6 FF 17-0978-10/-02
XK column 25/200 ml 36 mg/ml 90 Phenyl Sepharose 6 FF (high sub) 17-0973-10/-05
XK column 25/200 ml 24 mg/ml 90 Phenyl Sepharose 6 FF (low sub) 17-0965-10/-05
XK or Tricorn column 25/200 ml NA 34 Butyl Sepharose HP 17-5432-01/-02
XK or Tricorn column 75 ml 20 mg/ml 34 Phenyl Sepharose HP 17-1082-01
Quantity Binding capacity Particalesize (um) Product
Code number
Hydrophobicity : Butyl-S < Butyl < Octyl < Phenyl
HiTrap column5x1/1x5ml>90/450 mg ovalbumin90HiTrap Capto DEAE28-9165-37/-40
HiTrap column5x1/1x5ml75HiTrap Capto adhere 28-4058-44/-46
HiTrap column5x1/1x5ml>45/225 mg BSA at 30 mS/cm75HiTrap Capto MMC11-0032-73/-75
HiTrap column5x1/1x5ml>500 mg BSA90HiTrap Capto viralQ28-9078-09
HiTrap column5x1/1x5ml>120/600 mg lysozyme90HiTrap Capto S 11-5441-22/-23
HiTrap column5x1/1x5ml70/350 mg HSA34HiTrap Q HP17-1153-01/-1154-01
HiTrap column5x1/1x5ml55/275 mg ribonuclease A34HiTrap SP HP17-1151-01/-1152-01
HiTrap column5 x1 mlDepends on medium75 / 90HiTrap Capto IEX selection kit28-9343-88
HiTrap column5x1/1x5ml>100/500 mg BSA90HiTrap Capto Q 11-0013-02/-03
HiTrap column5x1/1x5ml160/900 mg lysozyme90HiTrap SP XL17-5160-01/-5161-01
HiTrap column5x1/1x5ml120/600 mg HSA90HiTrap Q FF17-5053-01/-5156-01
HiTrap column5x1/1x5ml130 /165 mg BSA90HiTrap Q XL17-5158-01/-5159-01
HiTrap column5x1/1x5ml50/250mg ribonuclease A90HiTrap CM FF17-5056-01/-5155-01
HiTrap column5x1/1x5ml43/215 mg BSA90HiTrap ANX FF(high sub)17-5162-01/-5163-01
HiTrap column5x1/1x5ml120/600 mg HSA90HiTrap DEAE FF17-5055-01/-5154-01
HiTrap column5x1/1x5ml70/350mg ribonuclease A90HiTrap SP FF17-5054-01/-5157-01
HiTrap column7 x1 mlDepends on medium34 or 90HiTrap IEX selection kit17-6002-33
Quantity Binding capacity
Particalesize (um)
Product Code number
Pre-packed Columns (Ion Exchange continue)
Sepharose
HiTrap column5x1/1x5mlLigand density:40umol/ml34HiTrap Phenyl HP17-1351-01/-5195-01
HiTrap column5x1/1x5mlLigand density:40umol/ml34HiTrap Butyl HP28-4110-01/-05
HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Octyl FF17-1359-01/-5196-01
RESOURCE column1 ml > 25 mg15 RESOURCE PHE17-1186-01
HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Butyl-S FF17-0978-13/-14
HiTrap column5x1/1x5mlLigand density:25umol/ml90HiTrap Phenyl FF (low sub)17-1353-01/-5194-01
HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Butyl FF17-1357-01/5197-01
HiTrap column5x1/1x5mlLigand density:40umol/ml90HiTrap Phenyl FF (high sub)17-1355-01/-5193-01
HiTrap column7 x1 mlDepends on medium34 or 90HiTrap HIC selection kit28-4110-07
RESOURCE column1 ml > 25 mg15 RESOURCE ETH17-1184-01
Quantity Binding capacity
Particalesize (um)
Product Code number
Capture
IntermediatePurification
Polishing
Res
olut
ion
Gel filtrationGel filtration Sepharose
Pre-packed Columns
HiLoad240 ml