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World J Microbiol Biottlchnol (20 I0) 26: 1125-1133 Journal
I.F =1.214
CHARACTERIZATION OF A SYNECHOCYSTIS SP FROM EGYPT WITH THE POTENTIAL OF BIOACTIVE COMPOUNDS PRODUCTION
Research Title
Nermin Adel El Semary & Mai Abd El Naby Authors
Department of Botany and Microbiology, Faculty of Science, Helwan University, Ain Helwan 11795, Egypt
e-mail: [email protected]; [email protected]
Affiliation
Abstract A polyphasic approach was employed to describe a unicellular coccoid cyanobacterium isolated from Terraat El Khashab, Helwan, Egypt. The cells were characterized by their small diameter (1.9-2.2 um) and lack of buoyancy. The cultures grew best at a temperature range of 20-40°C and moderate light intensity (20-50 umol photon m-2 s-I). To verify its cyanobacterial nature, sequencing of 16S rRNA gene using cyanobacterial specific primers was performed followed by a phylogenetic analysis. The sequence best-matched Synechocystis sp. PCC6803 with 90% similarity. The phylogenetic analysis placed the isolate within a major c1ade containing different Synechocystis isolates. The fatty acid composition was rich in saturated fatty acids while polyunsaturated fatty acids were scarce. The phytochemical screening revealed the presence of f1avenoids, alkaloids and saponins and absence of tannins. Vitamin C was also present in a considerable quantity. Some of the lipophilic fractions showed antimicrobial bioactivity against several pathogens. The pure bioactive compound from highest bioactive fraction was identified as oleic acid amide (M. wt.28 I) using chemical analyses including FIIIR, UV, proton H-NMR and GC-mass. The study highlights the importance of investigating the biotechnological potential of microorganisms inhabiting unusual niches.
Synechocystis, Polyphasic description' Bioactive compound, UV, FIIIR, Proton NMR, GC-mass and oleic acid amide
Key words
Faculty of Science 2010
Faculty of Science 2010
Ann Microbiol (2010) 60:181-188 Journal
I.F =0.350
INVESTIGATING FACTORS AFFECTING GROWTH AND CELLULAR MCYB TRANSCRIPTS OF MICROCY STIS AERUGINOSA PCC 7806USING REAL-TIME PCR
Research Title
Nermin Adel El Semary Authors Department of Botany and Microbiology, Faculty of Science, Helwan University,
Helwan 11795, Egypt, e-mail: [email protected] e-mail: [email protected]
Affiliation
Abstract The effects of temperature and light intensity on microcystis aeruginosa PCC 7806 growth and mcyB transcripts (as a function of microcystin biosynthesis) were investigated at the logarithmic and stationary growth phases. The expression of the mcyB gene--one of the microcystin biosynthetic genes-under different treatments was quantified using real-time PCR. The results showed that cell number increased at 25°C with all light treatments. A significant positive correlation (P=0.060) between mcyB transcripts and cell number was found, indicating the presence of coupling between toxin biosynthesis and growth. The results also revealed that both temperature and growth phase were significantly correlated with the number of mcyB transcripts (P=0.005 and 0.036, respectively), with at least 67% of the variation attributable to these two factors alone. On the other hand, the effect of the range of light intensities tested was overly non-significant. Application of high light intensity, above saturation point for a prolonged period, was inhibitory to transcription of the mcyB gene. Maximum mcyB transcript number was attained during stationary phase of a microcystis culture grown at 25°C under medium light intensity. The high level of mcyB transcription during stationary phase indicates an increase in the biosynthesis of micocystin. This possibly indicates the role of the toxin as an alarming chemical that cells would release upon lysis/death.
Microcystis aeruginosa, mcyB, Light Microcystin, QRT-PCR, Temperature Key words
Faculty of Science 2011
Annals of Microbiology, March 2012, Volume 62, Issue 1, pp 55-59 Journal
I.F =0.350
THE CHARACTERISATION OF BIOACTIVE COMPOUNDS FROM AN EGYPTIAN LEPTOLYNGBYA SP.STRAIN
Research Title
Nermin Adel El Semary Authors Department of Botany and Microbiology, Faculty of Science, Helwan University,
Helwan, Egypt 11795 e-mail: [email protected]
e-mail: [email protected]
Affiliation
Abstract An investigation into the bioactive metabolites from a benthic, mat-forming strain dominating a polluted wastewater canal in Egypt was conducted. Phytochemical screening revealed the presence of saponins, flavonoids and alkaloids; vitamin C was also found at high concentrations. The isolate was investigated as a source of antimicrobial compounds. The lipophilic fraction was extracted using chloroform/methanol and bioassays for antimicrobial compounds were performed using strains of pathogenic bacteria. The fraction that showed the highest bioactivity was purified and its structure elucidated using UV, FTIR, proton-NMR and GC-MS. The compound's molecular weight was 220 and it was identified as butylated hydroxytoluene which has both antimicrobial and antioxidant activities. On the ecological front, this compound, and the other metabolites detected, seem to enable the isolate to dominate its niche and protect it from adverse conditions. On the commercial front, this compound is used as a food additive and was recently discovered in different cyanobacteria, and can be used as a lead compound for both drug and food industries to substitute for the expensive and hazardous synthetic analogs. Therefore, this strain can be considered as a potential source of bioactive compounds that should be further explored.
Phytochemical screening- UV, FTIR, Proton-NMR, GC-MS, Butylated hydroxytoluene. Key words
Faculty of Science 2011
Molecules 2011,16,52-73; doi: 10.3390/moleculesI6010052 Journal
I.F =1.988
NOVEL SYNTHESIS AND ANTITUMOR EVALUATION OF POLYUNCTIONALLY SUBSTITUTED HETEROCYCLIC COMPOUNDS DERIVED FROM 2-CYANO-N- (3-CYANO -4,5,6,7-TETRAHYDROBENZO (b)THIOPHEN-2yl) - ACETAMIDE
Research Title
Hoda Z. Shams 1, Rafat M. Mohareb 2,3,* , Maher H. Helal 1 and Amira E. Mahmoud I Authors 1Department of Chemistry, Faculty of Science, Helwan University, Ain Helwan, Cairo,
Egypt 2 Department of Organic Chemistry, Faculty of Pharmacy, October University for
Modern Sciences and Arts, October City, Egypt
3 Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt * Author to whom correspondence should be addressed; E-Mail:
[email protected]; Tel.: +202-37626269 or +202-35676570.
Affiliation
Abstract The reaction of 2-amino-3-cyano-4,5,6, 7-tetrahydrobenzo[b]thiophene with ethyl cyanoacetate gave 2-cyano-N-(3-cyano-4,5,6, 7-tetrahydrobenzo[ b]thiophen-2-y I)-acetamide. The latter was used to synthesize different heterocyclic derivatives comprising thiophene, thiazole, pyrazole, pyridine, pyrimidine, and coumarin rings. The mechanistic and synthetic pathways depended on regioselective attack and/or cyclization by the cyanoacetamido moiety in the key precursor on various chemical reagents. The competition of the reaction pathways including dipolar cyclization, dinucleophilic-bielectrophilic attack, ~-attack, Gewald-type attack, and condensation reactions led to the diversity of the synthesized products. The antitumor activities of the synthesized products were studied and evaluated. Most of the compounds revealed high inhibitory effects when screened in vitro for their antiproliferative activity. Three human cancer cell lines, namely, breast adenocarcinoma (MCF-7), non-small cell lung cancer (NCI-H460) and CNS cancer (SF-268) were used in the screening tests. The simplicity of the synthetic procedures which mainly involved one-pot reactions under mild reaction conditions, the convenience of yield production and the diversity of the reactive sites in the produced systems play a valuable role for further heterocyclic transformations and further biological investigations.
4,5,6,7 –tetrahydrobenzo [ b] thiophene; thiazole; pyrazole; pyridine; antitumor Key words
The JOURNAL OF PHYSICAL CHEMISTRY C J. Phys. Chem. C, 2011, 115 (24), pp 12163–12172
Journal I.F =4.520
GROWTH AND REACTIVITY OF SILVER NANOPARTLCLES ON THE SURFACE OF TIO₂ A STOPPED-FLOW STUDY
Research Title
a Hanan H. Mohamed , b Ralf Dillert , and b Detlef W. Bahnemann Authors
a Chemistry Department, Faculty of Science, Helwan University, Helwan, Cairo, Egypt b Institut fur Technische Chemie, Leibniz Universitat Hannover, Callinstrasse 3, D-
30167, Hannover, Germany
Affiliation
Abstract: The reaction of electrons stored on TiO2 nanoparticles with silver ions in aqueous solution has been studied employing the stopped flow technique. Prior to the kinetic experiments, nanosized TiO2 particles were loaded with electrons by UV (A) photolysis in the presence of methanol. The formation of silver nanoparticles is detected by their typical surface plasmon (SP) absorbance band at 400 nm. Multiphase kinetic decay curves were observed for the electron absorbance as well as for the build-up of the plasmonic absorbance of the silver nanoparticles. This kinetic behavior is attributed to the multistep formation mechanism of the silver particles on the surface of TiO2 followed by the transfer of excess electrons to the deposited silver particles. The mechanism of the formation and growth of the silver particles on the TiO2 surface is proposed to be as following: (i) reduction of silver ions to form silver atoms which in turn form the nuclei for the metal particles, (ii) growth of the silver nuclei to form silver particles, and (iii) coalescence of the formed silver particles to form even bigger particles. Following the reduction of all silver ions present in solution, the remaining excess electrons are then transferred to the deposited silver particles resulting in a slight blue shift of the surface plasmon band. Subsequently, the stored electrons on the silver particles are used for the reduction of adsorbed H+ to produce H2 gas. The effect of Polyvinyl alcohol (PVA) as a stabilizer for the deposited metal nanoparticles as well as the effect of molecular oxygen on the unstabilized silver deposits have also been investigated. It could be shown that molecular oxygen acts as an electron acceptor, resulting in the partial oxidation of the deposited silver particles and thus a red shift and a damping of the surface plasmon absorbance band. The rate constants of the decay of the TiO2 electron absorbance as well as of the build-up of the plasmon absorbance of the silver nanoparticles have been measured.
Faculty of Science 2011
Faculty of Science 2011
The JOURNAL OF PHYSICAL CHEMISTRY A Journal
I.F =2.732
KINETIC AND MECHANISTIC INVESTIGATIONS OF MULTIELECTRON TRANSFER REACTIONS INDUCED BY STORED ELECTRONS IN TIO₂ NANOPARTICLES:A
STOPPED FLOW STUDY
Research Title
a Hanan H. Mohamed, b Cecilia B. Mendive, b Ralf Dillert, and c Detlef W. Bahnemann Authors a Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo,
Egypt b Institut fur Technische Chemie, Leibniz Universitat Hannover, Callinstrasse 3, D-
30167 Hannover, Germany c Departamento de Quimica, Facultad de Ciencias Exactas y Naturales, Universidad
Nacional de Mar del Plata, Dean Funes 3350, 7600 Mar del Plata, Argentina
Affiliation
Abstract: The kinetics and the mechanism of various multielectron transfer reactions initiated by stored electrons in TiO2 nanoparticles have been investigated employing the stopped flow technique. Moreover, the optical properties of the stored electrons in the TiO2 nanoparticles have been studied in detail following the UV (A) photolysis of deaerated aqueous suspensions of TiO2 nanoparticles in the presence of methanol. The reduction of common electron acceptors that are often present in photocatalytic systems such as O2, H2O2, and NO3− has been investigated. The experimental results clearly show that the stored electrons reduce O2 and H2O2 to water by multielectron transfer processes. Moreover, NO3− is reduced via the transfer of eight electrons evincing the formation of ammonia. On the other hand, the reduction of toxic metal ions, such as Cu(II), has been studied mixing their respective anoxic aqueous solutions with those containing the electrons stored in the TiO2 particles. A two-electron transfer is found to occur, indicating the reduction of the copper metal ion into its non toxic metallic form. Other metal ions, such as Zn(II) and Mn(II), could not be reduced by TiO2 electrons, which is readily explained on the bases of their respective redox potentials. The underlying reaction mechanisms are discussed in detail.
Faculty of Science 2011
Journal of Photochemistry and Photobiology A: Chemistry 217 (2011) 271-274 Journal
I.F =2.243
REACTION DYNAMICS OF THE TRANSFER OF STORED ELECTRONS ON TIO₂ NANOPARICLES A STOPPED FLOW STUDY
Research Title
a Mohamed Hanan H., b Dillert Ralf, and b Bahnemann Detlef W. Authors a Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo,
Egypt b Institut fur Technische Chemie, Leibniz Universitat Hannover, Callinstrasse 3, D-
30167 Hannover, Germany
Affiliation
Abstract The dynamics of the transfer of electrons from TiO2 nanoparticles to a variety of electron acceptors have been investigated employing a simple and facile stopped flow technique. Prior to the kinetic experiments nanosized Ti02 particles are loaded with electrons by UV (A) photolysis in the presence of methanol as a hole scavenger. As a model for possible electron transfer reactions the reduction of dissolved 02 and H2O2 by stored TiO2 electrons has been successfully studied.
Reaction Dynamic, Electron transfer, TiO2 nanoparticles, stopped flow Key words
Faculty of Science 2011
Journal of Hazardous Materials 186 (2011) 1527-1540
journal homepage: www:elsevier.comllocate/jhazmat
Journal I.F =2.054
EFFECT OF SOME CEMENT COMPONENTS ON ION CONTENTS IN DIFFERENT BRAIN AREAS OF ADULT MALE ALBINO MICE
Research Title
Abd el-Rahman M, el-Khadragy MF, Abd-el Hay H, Gab-Allah DM. Authors
Zoology Department, Faculty of Science, Helwan University, Cairo, Egypt. Affiliation
Abstract The aim of this study is to investigate the chronic effect of some cement components on the content of ions in different brain areas in adult male albino mice. It is clear that chronic intraperitoneal administration of 0.0013 mg/g aluminum ion caused a significant increase in aluminum. calcium and sodium ions and significant decrease in iron ions the chronic intraperitoneal administration of 0.00065 mg/g iron caused a significant increase in iron calcium and sodium ions but No significant change in potassium and aluminum ions. Chronic intraperitoneal administration of 0.0013 mg/g silicon caused no significant change in calcium potassium sodium aluminum and iron. Chronic intraperitoneal administration of 0.0013 rngjg aluminum. 0.0013 ttvglg silicon and 0.00065 mg/g iron respectively using separating time interval 30 min between each - caused a higher elevation in calcium sodium aluminum and iron concentrations than the elevation in other groups and no significant change in potassium ions. This may be due to the elevation in glutamate which leads to increase in the intracellular of calcium concentration and the inhibition of membrane-bound Na", K+. Ca2+ ATPase activity which lead to cellular alterations and may be death. So long-term exposure to cement components as environmental pollutants may lead to neurodegenerative diseases.
Aluminum Silicon Iron Cement Brain Ion contents Key words
Faculty of Science 2011
Molecules 2011, 16,6271-6305; doi: 10.3390/moleculesl 6086271 Journal
I.F =1.988
DESIGN AND SYNTHESIS OF NOVEL ANTIMICOBIL ACYCLIC AND HETEROCYCLIC DYES AND THEIR PRECURSORS FOR DYEING AND/OR TEXtile finishing based on
2-N-ACYLAMINO-4,5,6,7-TETRAHYDROBENZO b)THIOPHENE SYSTEMF
Research Title
Hoda Zaki Shams 1, Rafat Milad Mohareb 2,3,*, Maher Helmy Helal 1 and Amira EI-Sayed Mahmoud 1
Authors
1 Department of Chemistry, Faculty of Science, Helwan University, Ain Helwan, Cairo, P.O. 11790, Egypt
2 Department of Organic Chemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts, October City, P.O. 12613, Egypt
3 Department of Chemistry, Faculty of Science, Cairo University, Giza, P.O. 12311, Egypt
[email protected]; Tel.: +202-37626269 or +202-35676570.
Affiliation
Abstract A series of novel polyfunctionalized acyclic and heterocyclic dye precursors and their respective azo (hydrazone) counterpart dyes and dye precursors based on conjugate enaminones and/or enaminonitrile moieties were synthesized. The dyes and their precursors are based on 2-cyano-N-(3-cyano-4,5,6, 7-tetrahydrobenzo[b ]thiophen-2-yl)-acetamide, 2-ethoxycarbony I-N-(3-cyano-4,5,6, 7-tetrahydrobenzo[b]thiophen-2-y I)-acetamide or 2-pheny Icarbamoy I-N-(3-cyano-4,5,6, 7-tetrahydrobenzo[ b]thiophen-2-y I)-acetamide systems as precursors. The latter compounds were used to synthesize poly functional thiophene-, thiazole-, pyrazole, pyridine-, pyrimidine-, oxazine-, as well as acyclic moieties. The dyes and dye precursors were characterized by elemental analysis and spectral methods. All dyes and their precursors were screened in vitro and evaluated for both their antibacterial and antifungal activities. MIC data of the novel dye systems and their respective precursors showed significant antimicrobial activity against most tested organisms. Some compounds exhibited comparable or even higher efficiency than selected standards. Dyes were applied at 5% depth for disperse dyeing of nylon, acetate and polyester fabrics. Their spectral characteristics and fastness properties were measured and evaluated.
heterocyclic; dyes; antimicrobial activity; textile finish; fastness Key words
Faculty of Science 2011
Sensors 2011, 11, 1-x manuscripts; doi:10.3390/s110xOOOOx Journal
I.F =1.771
WEARABLE AND IMPLANTABLE WIRELESS SENSOR NETWORK SOLUTIONS EOR HEALTHCARE MONITORING
Research Title
AshrafDarwish1,* and Aboul Ella Hassanieu2 Authors 1 Faculty of Science, Helwan University, Cairo, Egypt
2 Faculty of Computers and Information, Cairo University, Cairo, Egypt E-Mails:[email protected];[email protected]
* Corresponding author. Tel.: +20105645222; fax: +20225552468
Affiliation
Abstract Wireless sensor network technologies are considered as one of the key research areas in computer science and healthcare application industries can be used for improving the quality of life. The purpose of this paper is to give a snapshot on current development and future direction of evolution in wearable and implantable body area network system for continuous monitoring for patients elsewhere. However, this paper explains the important role of body sensor networks in medicine which minimize the need for caregivers and help the chronically ill and elderly people to survive an independent life, besides provides quality care for people. The paper provides several state of the art examples together with the design considerations like unobtrusiveness, scalability, energy efficiency, security and also provides a comprehensive analysis of the benefits and drawbacks of these systems. Although having significant benefits, the area of wearable and implantable body sensor network has still major challenges and open research problems which are investigated and covered with proposed solutions in this paper.
Wireless sensor networks, body area networks, wearable sensors, implantable sensors, healthcare applications, biosensors, nanotechnology, privacy, security
Key words
Faculty of Science 2010
ACS Nano VOl. 4, NO.6, 3039-3044, 2010 Journal
I.F =9.855
AN OPTICAL NANOCAVITY INVORPORATING A FLUORESCENT ORGANIC DYE HAVING A HIGH QUALITY FACTOR
Research Title
Ali M.Adawt·* Mohamed M. Mursidyt.·§ PaulW. Fry,± and David G. Lidzeyt·* Authors department of Physics and Astronomy,The University of Sheffield, Hicks Building,
HounsfieldRoad,Sheffield53 7RH,United Kingdom, ±Nanoscience and Technology Centre, University of
Sheffield,NorthCampus,BroadLane,Sheffield,53 7HQ,UnitedKingdom, and
§Department of Physics ,Faculty of Science ,Helwan University, Helwan, Egypt
Affiliation
Abstract We have fabricated an l3 optical nanocavity operating at visible wavelengths that is coated with a thin-film of a fluorescent molecular-dye. The cavity was directly fabricated into a pre-etched, free-standing silicon-nitride (SiN) membrane and had a quality factor of Q = 2650. TIls relatively high Q-factor approaches the, theoretical limit that can be expected from an L3 nanocavity using silicon nitride asa dielectric material and is achieved as a result of the solvent-free cavity-fabrication protocol that we have developed .We show that the fluorescence from a red-emitting fluorescent dye coated onto the cavity surface undergoes strong emission intensity enhancement at a seriesof discrete wavelengths corresponding to the cavity modes .Three dimensional finite difference time domain (FDTD) calculations are used to predict the mode structure of the cavities with excellent agreement demonstrated between theory and experiment.
Optical nanocavity, photonic crystal, organic-semiconductor, fluorescent molecular-dye, finite difference time domain calculations
Key words
Faculty of Science 2010
Vol. 27, No 21, February 2010/J. Opt. Soc. Am. B Journal
I.F =2.095
THE OPTICAL PROPERTIES OF HYBRID ORGANIC-INORGANIC L3 NANOCAVITIES Research
Title
Mohamed M. Murshidy,1,3 Ali M. Adawi,l·4 Paul W. Fry,2 David M. Whittaker,1 and David G. Lidzey1,5
Authors
1Deportment of Physics and Astronomy, The University of Sheffield, Hicks Building, Hounsfield Road,
Sheffield S3 7RH, United Kingdom 2Nanoscience and Technology Centre, University of Sheffield, North Campus, Broad
Lane, Sheffield, S3 7HQ, United Kingdom
3Department of Physics, Faculty of Science, Helwan University, Helwan Egypt 4Email: [email protected] 5Email: [email protected]
Affiliation
Abstract We report the fabrication of L3 nanocavities defined into a free-standing silicon nitride (SiN) membrane coated with a thin (10 nm) film of fluorescent red-emitting conjugated polymer. We find that structures both with and without the conjugated polymers are characterized by a number of different confined optical modes with quality factors ranging between 450 and 1200. We characterize the polarization of the modes and discuss the enhancement of emission intensity from both the SiN and the polymer using spectral imaging.
Faculty of Science 2010
APPLIED PHYSICS LEITERS 97, 153303 (2010) Journal
I.F =3.820
A ONE DIMENSIONAL PHOTONIC CRYSTAL NANOCAVITY INCORPORATING FLUORESCENT MOLECULAR DYE
Research Title
Mohamed M. Murshidy,1,2 AIi M. Adawi,1.3,a Paul W. Fry,4 and David G. Lidzeyl,b Authors IDepartment of Physics and Astronomy, The University of Sheffield. Hicks Building,
Hounsfield Road, Sheffield S3 7RH, United Kingdom 2Department of Physics, Faculty of Science, Helwan University, P'O.B. 11795, Helwan,
Egypt 3Departmenl of Physics, University of Hull, Cottingham Road, Hull HU6 7RX, United
Kingdom 4Nanoscience and Technology Centre, University of Sheffield, North Campus, Broad
Lane, Sheffield, S3 7HQ, United Kingdom
Affiliation
Abstract We model the optical properties of a SiN based one-dimensional waveguide photonje crystal nanocavity with three tapered air holes as a function of the photonic crystal mirror fill factor using finite difference time domain calculations. We show that the Q-factor of this type of cavity has a strong dependence on the mirror fill factor, with a Q-factor as high as 35000 predicted. Experimental studies on a range of representative structures whose surface is coated with a layer of a fluorescent dye con film our modeling and reveal that such structures are characterized by a single optical mode having a Q-factor of 2000.
Faculty of Science 2011
International Journal of Geometric Methods in Modern Physics
Volume 08, Issue 01, February 2011
Journal I.F =2.054
ON THE SOLUTION SETS OF FOUR-POINT BOUNDARY VALUE PROBLEMS FOR NONCONVEX DIFFERENTIAL INCLUSIONS
Research Title
Adel Mahmoud Gomaa Authors
Mathematics Department, Faculty of Science, Helwan University, Cairo, Egypt Affiliation
Abstract:
We consider the multivalued problem under four boundary conditions u(0) = x0, u(η) = u(θ) = u(T) where 0 < η < θ < T and for F is a multifunctions from [0, T] × ℝn × ℝn to the nonempty compact subsets of ℝn not necessary convex. We give a lemma which is useful in the study of four boundary problems for the differential equations and the differential inclusions. Further we have results that improve earlier theorems.
Differential inclusions, four-point boundary value problems, solution sets, Creen functions, Second derivatives.
Key words
Faculty of Science 2010
International Journal of Geometric Methods in Modern Physics
Volume 07, Issue 03, May 2010 Journal
I.F =0.757
ON BOUNDED WEAK A D PSEUDO-SOLUTIONS OF NO LI EAR DIFFERENTIAL EQUATIONS HAVING TRICHOTOMY WITH AND WITHOUT DELAY IN BA ACH
SPACES
Research Title
Adel Mahmoud Gomaa Authors Mathematics Department, Faculty of Science, Helwan University, Cairo, Egypt Affiliation
Abstract In the present work we consider E is a Banach space, E* is its dual space and L(E) is the space of continuous linear operators from E to itself. A function x: ℝ → E is said to be a pseudo-solution of the
equation where A:ℝ → L(E) is strongly measurable and Bochner integrable function on every finite subinterval of ℝ with f:ℝ × E → E is only assumed to be weakly
weakly sequentially continuous or Pettis-integrable and the linear equation has a trichotomy with constants α ≥ 1 and σ > 0, if x is absolutely continuous function and for each x* ∈ E* there exists a negligible set ℵx* such that for each t ∉ ℵx*, then we have
We give an existence theorem for bounded weak and pseudo-
solutions of the nonlinear differential equations Let T, r, d > 0, Br = {x > E: ‖ x‖ ≤ r} and CE([-d,0]) be the Banach space of continuous functions from [-d,0] into E. Finally we prove an existence result for the differential equation with delay
where fd : [a,b] × CE([-d,0]) → E is weakly weakly sequentially
continuous function, is strongly measurable and Bochner integrable operator on [a,b] and θtx(s) = x(t + s) for all s ∈ [-d,0].
Nonlinear differential equations; weak solutions; pseudo-solutions; trichotomy; delay Key words
Faculty of Science 2010
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume 91, June 2012, Pages 11–17
Journal I.F =1.770
PREPARATION, SPECTROSCOPIC AND THERMAL CHARACTERIZATION OF NEW METAL COMPLEXES OF VERLIPRIDE DRUG. IN VITRO BIOLOGICAL ACTIVITY
STUDIES
Research Title
M.H. Soliman a, Gehad C Mohamed b Authors a Chemistry Department, Faculty of Science, Helwan University, Helwan, Egypt b Chemistry Department. Faculty of Science, Cairo University, 12613 Giza, Egypt
Affiliation
Abstract: Metal complexes of the general formula [M(VER)2Cl2(H2O)2]·yH2O and [Cr(VER)2Cl2(H2O)2]Cl·H2O (where VER = verlipride, M = Mn(II) (y = 2), Co(II) (y = 2), Ni(II) (y = 2), Cu(II) (y = 1) and Zn(II) (y = 0)) are prepared and characterized based on elemental analyses, IR, 1H NMR, magnetic moment, molar conductance, and thermal analyses (TG and DTA) techniques. From the elemental analyses data, the complexes are formed in 1:2 [Metal]:[VER] ratio. The molar conductance data reveal that all the metal chelates are non-electrolytes except Cr(III) complex, it is 1:1 electrolyte. IR spectra show that VER is coordinated to the metal ions in a neutral monodentate manner with O donor site of the carbonyl O atom. On the basis of spectral studies and magnetic moment measurements an octahedral geometry has been assigned for the complexes. The thermal behavior of these chelates is studied using thermogravimetric analysis technique. The results obtained show that the complexes lose hydrated water, HCl and coordinated water molecules followed immediately by decomposition of the ligand molecules in the successive unseparate steps. The VER drug, in comparison to its metal complexes is also screened for its biological activity against Gram positive bacterial (Staphylococcus aureus) and Gram negative bacteria (Escherichia coli) and fungi (Candida albicans and Aspergillus flavus) in vitro. The activity data show that most of the metal complexes have antibacterial activity like or higher than that of the parent VER drug against one or more species.
Verlipride Meral complexes IR Molar conductance Magnetic moment H NMR Thermal analyses Biological activity
Key words
Faculty of Science 2010
Z Naturforsch C. 2010 Sep-Oct; 65(9-10):577-87. Journal
I.F =2.054
A NEW CLASS OF ANTIMETABLITES PYIDINE THIOGLYCOSIDESAS POTENTIAL ANTICACER AGENTS
Research Title
Elgemeie GH, Mahdy EM, Elgawish MA, Ahmed MM, Shousha WG, Eldin ME. Authors Faculty of Science, Chemistry Department, Helwan University, Helwan, Cairo, Egypt.
[email protected] Affiliation
Abstract: The present study was designed for highlighting and focusing on the cytotoxic activity of a new class of antimetabolites both on human cell lines, namely liver carcinoma cell line (Hepg2), lung carcinoma cell line (H460), breast carcinoma cell line (MCF7), brain carcinoma cell line (U251), and animal cell line EAC (Ehrlich ascites carcinoma cells). The results revealed that some of these modified deazapyrimidine thioglycosides have significant cytotoxic activity against EAC cells with growth inhibition percentage ranged between 80% to 90%. The possible inhibitory mechanism of the pyridine thioglycosides was explored by studying the cell cycle perturbation of thioglycosides against human cell lines (in vitro) as well as the most suitable time for maximum compound cytotoxic activity after 6, 18, and 24 h of incubation. To confirm the cytotoxic activity of these compounds, they have been tested for their apoptotic and antiproliferative activity in vivo against solid Ehrlich tumours using five groups of Swiss albino mice for 37 days from inoculation and three treatments, 250, 500 and 1000 microg/kg body weight. There was significant reduction in Ehrlich tumour size in case of the 500 and 1000 microg/kg body weight group but mild significant tumour reduction in the 250 microg/kg body weight group. Histograms of DNA per cell for each treatment group indicated that there was a dose-dependent increase in the preG1 phase with a corresponding complete arrest of cells from entering the G2/M phase compared to the untreated EAC group. In conclusion, pyridine thioglycosides have proven good cytotoxic effects against EAC cells and also significant cytotoxic activity against the four tested human cell lines. Flow cytometric DNA ploidy analysis of pyridine thioglcyosides against the Hepg2 and U251 cell lines revealed that the postulated mechanism of action of pyridine thioglcyosides is cell cycle arrest in the S phase. This is similar to antimetabolites and cell cycle arrest in the G2/M phase (M phase) in the same way as microtubule inhibitors like pyridine thioglycosides are cell-cycle-specific in the S phase and the M phase (in case of human cell lines) and have apoptotic effects (in case of animal cell line).
Anticancer agents, antimetabolities, Pyridine Thioglycosides Key words
Faculty of Science 2011
Journal of Gastroenterology and Hepatology
Volume 26, Issue 5, pages 843–850, May 2010
Journal I.F =2.410
SINGLE NUCLEOTIDE POLYMERPBTSM AT EXON 7 SPLIC ACCEPTOR SITE OF OAS1 GENE DETER MINES RESPONSE OF HEPATITS C VIRUS PATIENTS TO
INTERAPY
Research Title
Mostafa K El Awady,* Mohamed A Anany,* Gamal Esmat,† Naglaa Zayed,† Ashraf A Tabll,*
Amr Helmy,‡ Abdel Rahman El Zayady,• Mohga S Abdalla, Hayat M Sharada, Maissa El Raziky,†Wafaa El Akel,† Shadia Abdalla** and Noha G Bader El Din*
Authors
*Department of Microbial Biotechnology, National Research Center, and †Department of Tropical Medicine Gastroenterology and Liver Diseases, Kasr El Aini, and §Cairo
Liver Center, and **Department of Clinical Pathology, El Sahel Teaching Hospital, Cairo, and ‡National Liver Institute, Shebeen El Kom, EL Monufea, and ¶Faculty of Sciences
Helwan University, Helwan, Egypt
Affiliation
Abstract: Background and Aim: Response to interferon therapy and disease progression in hepatitis C virus (HCV) infected patients differs among individuals, suggesting a possibility of a contribution of host genetic factors. 2′-5′-oligoadenylate synthetase 1 (OAS1), an important component of the innate immune system with a proven antiviral function, may therefore have a relationship with the response to interferon therapy and clinical course of HCV disease. Our aim was to determine the frequency of single nucleotide polymorphism (SNP) at exon 7 splice acceptor site (SAS) of the OAS1 gene in relation to the interferon response and status of HCV infection. Methods: A 203 bp fragment containing exon 7 SAS was amplified in 70 HCV chronic patients and 50 healthy controls. SNP was examined using restriction fragment length polymorphism (RFLP) genotyping method. Correlations of SNP genotypes with response to interferon and clinical status of patients were statistically analyzed. Results: There was an increasing trend of response from AA to AG to GG genotypes (P = 0.007). Genotype AA was associated with non-response to interferon and higher degree of liver fibrosis (P = 0.05). Multivariate analysis showed this SNP as independent and a significant determinant of the outcome of interferon therapy (odds ratio 4.913 [95% confidence interval 1.365–8.2], P = 0.006). Conclusions: This is the first study to show a significant association between the functional SNP at exon 7 SAS of OAS1 gene and the viral response to interferon in chronic HCV patients. Patients with AA genotype were associated with progressive HCV disease and viral resistance to interferon therapy. This OAS SNP is a potential bio-marker to predict IFN response in chronic hepatitis C patients.
Hepatitis C virus; iver fibrosis; OAS1; response to interferon therapy; single nucleotide polymorphism
Key words
Faculty of Science 2011
J Nutr Biochem. 2012 Jan;23(1):39-50. Epub 2011 Mar 16. Journal
I.F =4.538
ACUTE AND CHTONIC SATURATED FATTY ACID TREATMENT AS A KEY INSTIGATOR OF THE TLR-MEDIATED INFAMMATORY RESPONSE IN HUMAN
ADIPOSE TISSUE,IN VITRO
Research Title
Elham M. Youssef-Elabd,a Kirsty C. McGee,b Gyanendra Tripathi,b Nasser Aldaghri,f Mohga S. Abdalla,c Hayat M. Sharada,c Esmat Ashour,a Ashraf I. Amin,d Antonio
Ceriello,e Joseph P. O'Hare,b Sudhesh Kumar,b Philip G. McTernan,b and Alison L. Harteb,
Authors
aBiochemistry Department, National Research Centre, Dokki, Giza, Egypt 12622 bUniversity of Warwick, Unit for Diabetes and Metabolism, Warwick Medical School, Clinical
Sciences Research Institute, UHCW, Coventry, UK CV2 2DX cChemistry Department, Faculty of Science, Helwan University, Egypt 11795
dClinical Pathology Department, National Institute of Diabetes and Endocrinology, Cairo, Egypt 11562
eInsititut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Calle Mallorca 183, Piso P01, 08036 Barcelona, Spain
fKing Saud University, College of Science Biochemistry Department, Riyadh 11451, Saudi Arabia
Alison L. Harte: [email protected] Corresponding author. Tel.: +44 24 76968645; fax: +44 24 76968653 ; Email:
Affiliation
Abstract A post-prandial increase in saturated fatty acids (SFAs) and glucose (Glc) activates an inflammatory response, which may be prolonged following restoration of physiological SFAs and Glc levels--a finding referred to as 'metabolic memory'. This study examined chronic and oscillating SFAs and Glc on the inflammatory signalling pathway in human adipose tissue (AT) and adipocytes (Ads) and determined whether Ads are subject to "metabolic memory." Abdominal (Abd) subcutaneous (Sc) explants and Ads were treated with chronic low glucose (L-Glc): 5.6 mM and high glucose (H-Glc): 17.5 mM, with low (0.2 mM) and high (2 mM) SFA for 48 h. Abd Sc explants and Ads were also exposed to the aforementioned treatment regimen for 12-h periods, with alternating rest periods of 12 h in L-Glc. Chronic treatment with L-Glc and high SFAs, H-Glc and high SFAs up-regulated key factors of the nuclear factor-κB (NFκB) pathway in Abd Sc AT and Ads (TLR4, NFκB; P<.05), whilst down-regulating MyD88. Oscillating Glc and SFA concentrations increased TLR4, NFκB, IKKβ (P<.05) in explants and Ads and up-regulated MyD88 expression (P<.05). Both tumor necrosis factor α and interleukin 6 (P<.05) secretion were markedly increased in chronically treated Abd Sc explants and Ads whilst, with oscillating treatments, a sustained inflammatory effect was noted in absence of treatment. Therefore, SFAs may act as key instigators of the inflammatory response in human AT via NFκB activation, which suggests that short-term exposure of cells to uncontrolled levels of SFAs and Glc leads to a longer-term inflammatory insult within the Ad, which may have important implications
for patients with obesity and Type 2 diabetes.
